CN117678605A - Application of Gu Weijun element in regulating plant growth and application method thereof - Google Patents
Application of Gu Weijun element in regulating plant growth and application method thereof Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 230000008635 plant growth Effects 0.000 title claims abstract description 11
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 8
- 235000013399 edible fruits Nutrition 0.000 claims description 46
- 239000011248 coating agent Substances 0.000 claims description 19
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 18
- 230000035784 germination Effects 0.000 claims description 17
- 230000001737 promoting effect Effects 0.000 claims description 17
- 230000012010 growth Effects 0.000 claims description 15
- 238000007598 dipping method Methods 0.000 claims description 10
- 238000000576 coating method Methods 0.000 claims description 9
- 230000033228 biological regulation Effects 0.000 claims description 6
- 230000002786 root growth Effects 0.000 claims description 5
- 230000014759 maintenance of location Effects 0.000 claims description 4
- 230000008121 plant development Effects 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
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- 238000002791 soaking Methods 0.000 claims description 3
- 230000007226 seed germination Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 16
- 239000005648 plant growth regulator Substances 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 55
- 235000013311 vegetables Nutrition 0.000 description 31
- 240000003768 Solanum lycopersicum Species 0.000 description 23
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 14
- 240000007124 Brassica oleracea Species 0.000 description 10
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 10
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 10
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 10
- 240000007594 Oryza sativa Species 0.000 description 9
- 235000007164 Oryza sativa Nutrition 0.000 description 9
- 235000021028 berry Nutrition 0.000 description 9
- 235000013339 cereals Nutrition 0.000 description 9
- 235000009566 rice Nutrition 0.000 description 9
- 241000219198 Brassica Species 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 235000011331 Brassica Nutrition 0.000 description 6
- 241000219112 Cucumis Species 0.000 description 6
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 6
- 244000061458 Solanum melongena Species 0.000 description 6
- 235000002597 Solanum melongena Nutrition 0.000 description 6
- 241000209140 Triticum Species 0.000 description 6
- 235000021307 Triticum Nutrition 0.000 description 6
- 240000008042 Zea mays Species 0.000 description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 6
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- 235000020971 citrus fruits Nutrition 0.000 description 6
- 239000004575 stone Substances 0.000 description 6
- 241001593968 Vitis palmata Species 0.000 description 5
- 238000011161 development Methods 0.000 description 5
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- 235000005822 corn Nutrition 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
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- 239000000243 solution Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 239000002270 dispersing agent Substances 0.000 description 3
- 239000002562 thickening agent Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000000080 wetting agent Substances 0.000 description 3
- 235000011305 Capsella bursa pastoris Nutrition 0.000 description 2
- 240000008867 Capsella bursa-pastoris Species 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
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- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 108010073254 Colicins Proteins 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 244000203593 Piper nigrum Species 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Life Sciences & Earth Sciences (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The application of Gu Weijun element in regulating plant growth and the application method thereof provided by the application provides that the application of Gu Weijun element, which is a novel plant growth regulator, digs various effects, and expands the application scene of Gu Weijun element.
Description
Technical Field
The invention belongs to the technical field of plant protection, and particularly relates to application of Gu Weijun in regulating plant growth and a using method thereof.
Background
Gu Weijun (also known as wugufensu, WGFs, chemical name (2R, 3R, 4S) -2- (6-amino-9H-purin-9-yl) -2- (hydroxymethyl) -5-methylenetetrahydrofuran-3, 4-diol, molecular formula:
C 11 H 13 N 5 O 4， the structural formula is shown as formula I), and is a novel nucleoside compound obtained by separating an agricultural microbial pesticide innovation team of northeast agricultural university from fermentation liquor of actinomycete streptomyces darkling ash mould NEAU 6.
At present, gu Weijun element has the functions of promoting germination, rooting and the like of rice and has good plant growth regulating effect. However, there are few reports of application to other crops, and different application periods, application concentrations, application sites, etc. are also closely related to physiological actions and biological activities exerted by the same type or type of crops. Therefore, research and development of application technology and specific application effects (i.e., uses) of the oryzanol in various crops are needed.
Disclosure of Invention
In order to solve the technical problems, the application of Gu Weijun element in crop growth regulation is provided.
In particular, one aspect of the invention resides in the use of Gu Weijun in improving plant leaf quality, including leaf weight and leaf number.
In another aspect, the invention resides in the use of Gu Weijun in plant flower forcing and preserving, including early flowering, reduced flower fall, and increased flower yield.
In another aspect of the invention, the application of Gu Weijun in fruit retention and fruit swelling, fruit cracking prevention, sweetening and color turning of plants is provided.
In another aspect, the invention resides in the use of Gu Weijun in plant type control, including regulating plant development, increasing plant height, increasing stem thickness.
In another aspect of the invention, the Gu Weijun element is used in promoting seed bud growth, increasing fresh weight of seed, increasing fresh weight of seedling, promoting root growth, increasing germination vigor, increasing germination rate of seed and shortening transplanting time.
In another aspect of the invention, methods of modulating plant growth using Gu Weijun are provided.
Gu Weijun plants include cereal crops, potato crops, cabbage vegetables, root vegetables, eggplant vegetables, melon vegetables, green leaf vegetables, stone fruits, kernel fruits, berries, citrus fruits, nuts, and brassica.
Preferably, the plant is one or more of a solanaceous vegetable, a cereal crop, a brassica plant or a berry, more preferably, the plant is one or more of tomato, eggplant, capsicum, cabbage, rape, shepherd's purse, brassica, green vegetable, rice, maize, wheat, grape seed.
Further, the application period of Gu Weijun includes, but is not limited to, vegetative and reproductive periods, preferably a needle setting period, a post-transplanting growth period, a jointing period, a pre-flowering period, a post-transplanting seedling-reviving period, a rhizome expanding period, a jointing period, a tillering period, a seedling period, a bud setting period, a flowering period, a blushing period, a jointing period, an ear alignment period, a flowering period, a fruit expanding period, a fruit turning period, a germination period, a young fruit period.
Further, in the present invention, the Gu Weijun element treated plant parts include, but are not limited to, whole plants or plant organs such as roots, stems, leaves, flowers or fruits, or plant seeds.
In another aspect of the invention there is provided a method of regulating plant growth or seed development with Gu Weijun comprising the steps of: gu Weijun is applied to the seeds, plant parts and/or whole plants of the plant.
Further, the application method is selected from seed soaking, dipping, seed dressing, coating, spot coating, spraying, atomizing, root dipping, flower dipping, fruit dipping, root irrigation and the like.
The invention has the beneficial effects that:
the Gu Weijun provided by the application is applied to the aspects of improving the quality of plant leaves, promoting flowers and keeping flowers, controlling plant types, promoting the growth of seed buds, improving the fresh weight of seeds, improving the fresh weight of seedlings, promoting the growth of seed roots, improving the germination vigor, improving the germination rate of seeds, shortening the transplanting time and the like, so that various different effects of the novel plant growth regulator Gu Weijun are developed, and the application scene of Gu Weijun is expanded.
Drawings
Fig. 1 is a graph showing the effect of adjusting the quality of cabbage leaves with Gu Weijun elements at different concentrations, wherein a graph A shows the change of the quantity of cabbage leaves treated with Gu Weijun elements at different concentrations, and a graph B shows the change of the average weight of cabbage leaves treated with Gu Weijun elements at different concentrations.
FIG. 2 shows development of tomato treated with Gu Weijun elements at different concentrations, wherein A-1, A-2 and A-3 are ddH respectively 2 Phenotype of tomato plants after 51 days of treatment with O, 250. Mu.M and 500. Mu.M concentrations of Gu Weijun elements; FIGS. A-4, A-5 and A-6 are ddH, respectively 2 Results at inflorescence 2 of plants treated with O, 250. Mu.M and 500. Mu.M concentrations of Gu Weijun elements; panel B shows tomato plant heights at day 22 and day 51 after treatment with different concentrations of Gu Weijun elements; panel C is the stem thickness of tomatoes at 22 and 51 days after treatment with different concentrations of Gu Weijun elements; panel D shows flowering rates of tomatoes at day 22 after treatment with different concentrations of Gu Weijun elements; panel E shows average fruit number of tomato plants on day 51 after treatment at different concentrations; panel F shows the yield of the first tomato harvest and panel G shows the average yield of the final tomato plant.
FIG. 3 shows the development of wheat seeds treated with different amounts of Gu Weijun element coating.
FIG. 4 shows the development of rice seeds treated with different amounts of Gu Weijun element coating agent.
FIG. 5 shows the development of corn seeds treated with various amounts of Gu Weijun element coating.
Fig. 6 shows the development of red grape fruits treated with Gu Weijun element solutions of different concentrations, wherein fig. a shows the effect of Gu Weijun element solutions of different concentrations on the length of red grape fruits, and fig. B shows the effect of Gu Weijun element solutions of different concentrations on the number of red grape fruits.
Detailed Description
For the purposes of the present invention, the terms used in this application have the following meanings, unless otherwise indicated:
the term "modulating plant growth" refers to compounds or compositions having the effect of modulating a plant development program, which is capable of causing a change in plant growth. Examples include, but are not limited to, promoting or inhibiting germination, rooting, flower bud differentiation, flowering, fruiting, defoliation, and the like.
In some embodiments, the use of Gu Weijun in improving plant leaf quality, including at least one of leaf weight or leaf number, wherein leaf weight can be manifested as an increase in leaf thickness or an increase in leaf area; the plant comprises at least one of cereal crops, tuber crops, cabbage vegetables, straight root vegetables, eggplant vegetables, melon vegetables, green leaf vegetables, stone fruits, kernel fruits, berries, citrus fruits, nuts and brassica.
Preferably, the plant is a brassica plant, including at least one of cabbage, rape, shepherd's purse, brassica napus or green vegetable.
The Gu Weijun element dosage concentration is 10-100ppm, preferably 30-70ppm or 40-60ppm and the numerical range between any two values.
In some embodiments, the use of Gu Weijun in flower-promoting and flower-preserving plants, including at least one of early flowering, reduced flower-fall, and increased flower yield, the plants used include at least one of cereal crops, yam crops, cabbage vegetables, straight root vegetables, eggplant vegetables, melon vegetables, green leaf vegetables, stone fruits, kernel fruits, berries, citrus fruits, nuts, brassica.
Preferably, the plant is a solanaceous vegetable or berry fruit.
In the application of promoting and preserving flowers of berries, the administration concentration of Gu Weijun element is 10-100ppm, more preferably 20-80ppm,50-100ppm or 30-70ppm and the numerical range between any two values; in the application of the solanaceous vegetables for promoting and preserving flowers, the administration concentration of Gu Weijun is not lower than 100 mu M, preferably not lower than 250 mu M, more preferably 100 mu M, 200 mu M, 250 mu M, 300 mu M, 400 mu M or 500 mu M and the numerical range between any two values.
In some embodiments, the Gu Weijun element is used in plant type control comprising at least one of regulating plant development, increasing plant height, increasing stem thickness, the applied plant comprising at least one of cereal crops, yam crops, cabbage vegetables, straight root vegetables, solanaceous vegetables, melon vegetables, green leaf vegetables, stone fruits, kernel fruits, berries, citrus fruits, nuts, brassica.
Preferably, the plants used comprise solanaceous vegetables or cereal crops. When the plant type control method is used for plant type control of solanaceous vegetables, the plant type control method is not lower than 250 mu M, preferably a numerical range between any two values of 250 mu M, 300 mu M, 400 mu M and 500 mu M. For plant type control of cereal crops, the Gu Weijun element is used in an amount of 0-0.03%, preferably 0-0.02%, or 0-0.01%, preferably 0.005%,0.002%, 0.001%, 0.0005%, or 0.0001% -0.01% by weight of the total mass of seeds, and a numerical range between any two values.
In some embodiments, the Gu Weijun is used to promote seed bud growth, increase fresh weight of seeds, increase fresh weight of seedlings, promote root growth of seeds, increase vigor, increase germination rate of seeds, the applied plants including at least one of cereal crops, yam crops, cabbage vegetables, straight root vegetables, solanaceous vegetables, melon vegetables, green leaf vegetables, stone fruits, kernel fruits, berries, citrus fruits, nuts, brassica. In the application of improving the germination vigor and/or the germination rate of seeds, the Gu Weijun element is used in an amount of not more than 0.02%, preferably not more than 0.01%, more preferably 0.0001% -0.01%, or preferably 0.02%, 0.01%, 0.005%,0.002%, 0.001%, 0.0005%, or a range of values between any two values of 0.0001% based on the total mass of seeds. In applications where growth of seed sprouts, root growth of seeds and/or fresh weight of seeds is to be increased, the cereal colicin is administered at a concentration of 10-100ppm, more preferably 10-50ppm, and even more preferably 10ppm, 20ppm, 30ppm, 40ppm, 50ppm, ranging between any two values. In applications where the weight of the seedling is raised or the growth of the seed root is promoted, the Gu Weijun element is used in an amount of not more than 0.005%, preferably 0.0001% to 0.005%, or preferably 0.005%, 0.004%, 0.003%, 0.002%, 0.001%, 0.0005%, or 0.0001% of any range between any two values.
In some embodiments, the Gu Weijun element is applied to the fruit retention and swelling of plants, wherein the applied plants comprise at least one of solanaceous vegetables, melon vegetables, legume vegetables, stone fruits, kernel fruits, berries and citrus fruits; the Gu Weijun element is administered at a concentration of 10-200ppm, preferably 10-100ppm, preferably 30-70ppm, or preferably in a range between any two of 30ppm, 40ppm, 50ppm, 60ppm, 70 ppm. .
In some embodiments, the Gu Weijun element application period includes, but is not limited to, a vegetative and reproductive period, preferably a post-transplant growth period, a pre-flowering period, a post-flowering period, a tillering period, a seedling period, a bud period, a flowering period, a fruit expansion period, a fruit color transfer period, a germination period, a young fruit period.
In some embodiments, the Gu Weijun element treated plant parts include, but are not limited to, whole plants or plant organs, such as roots, stems, leaves, flowers or fruits, or plant seeds.
In some embodiments, there is provided a method of Gu Weijun element modulating plant growth or seed development comprising the steps of: gu Weijun is applied to the seeds, plant parts and/or whole plants of the plant.
In some embodiments, the method of administering the composition comprises one or more of seed soaking, seed dressing, coating, seed spraying, root dipping, flower dipping, fruit dipping, and root irrigation.
Example 1: gu Weijun Regulation of white vegetable growth
Experimental materials: chinese cabbage seedling.
Gu Weijun: 30ppm/50ppm/70ppm concentration.
The experimental method comprises the following steps: cleaning and sterilizing Chinese cabbage seeds, sowing in a small basin,spraying Gu Weijun element solution with different concentration on cabbage seedling after 21 days, wherein the dosage is about 20 mL/plant, 20 seedlings are treated each, and ddH is used 2 O is a blank control group (CK), and leaf number and fresh weight were measured 15 days after spraying.
Experimental results: the experimental results are shown in FIG. 1, wherein the average leaf number of the CK group is 9.25, and the average weight of each leaf is 12.03g; the average leaf number of the cabbage treated by the 30ppm Gu Weijun element solution is 10.0, the leaf weight is increased by 8.11 percent relative to the CK group, the average leaf weight is 16.00g, and the leaf weight is increased by 33 percent relative to the CK group; the average leaf number of the cabbage treated by the 50ppm Gu Weijun element solution is 11.5, the leaf weight is increased by 24.3 percent relative to the CK group, the average leaf weight is 30.56g, and the leaf weight is increased by 154 percent relative to the CK group; the average leaf number of cabbage treated with 70ppm Gu Weijun element solution is 10.0, 8.11% relative to CK group, and the average leaf weight per leaf is 23.85g, 99% relative to CK group. It can be seen that Gu Weijun element has a significant promoting effect on plant leaf quality, wherein the leaf quality after 50ppm concentration treatment is the highest.
Example 2: gu Weijun effect of element on tomato growth regulation
Experimental materials: tomato plants in seedling stage and flowering stage.
Gu Weijun: a solution having a concentration of 250. Mu.M/500. Mu.M.
The experimental method comprises the following steps: spraying oryzanol twice on tomato plants in seedling raising period and flowering period, wherein the seedling raising period is about 20 mL/plant and the flowering period is about 80 mL/plant, 50 plants are treated each, and ddH is used 2 O is a blank control group (CK), indexes such as plant height, stem thickness, flowering rate, fruiting rate and the like are counted on the 22 th day and the 51 th day after the first treatment, namely, the flowering period and the fruiting period of tomatoes, and the yield of the first harvested tomatoes and the average yield of the final single tomato plant are counted on the fruiting period (the 51 th day).
Experimental results: as can be seen from comparison of fig. 2A-1 to 2A-3, the tomato plant after being treated with Gu Weijun elements has a higher plant type and a higher plant type expansion degree in the same growth period (day 51); as can be seen from fig. 2A-4 to fig. 2A-6, the tomato fruits treated with Gu Weijun elements are significantly larger and more than CK groups and the flowering numbers are also more than CK groups in the 2 nd inflorescence period of the plants, which indicates that Gu Weijun elements contribute to early flowering, reduced flower fall, improved flower formation rate and fruit retention and fruit swelling effects of the plants. As can be seen from FIG. 2B, the tomato plant heights after 22 days of treatment with Gu Weijun elements at 250. Mu.M and 500. Mu.M increased by 23.62% and 32.51% respectively, and the tomato plant heights after 51 days of treatment with 250. Mu.M and 500. Mu.M increased by 8.77% and 13.24% respectively, relative to the CK component. As can be seen from FIG. 2C, the tomato stem thickness after 22 days of treatment with 250. Mu.M and 500. Mu.M was increased by 25.58% and 37.21% relative to CK, respectively, and the tomato stem thickness after 51 days of treatment with 250. Mu.M and 500. Mu.M was substantially equivalent, with an increase of about 17.81% relative to CK. As can be seen from FIG. 2D, the flowering rates of the tomatoes after 22 days of treatment with 250. Mu.M and 500. Mu.M were 83.33% and 84.67%, respectively, and the control flowering rate was 61.25%. As can be seen from fig. 2E, the tomato fruits on day 51 after treatment with Gu Weijun elements at concentrations of 250 μm and 500 μm are substantially equivalent, which is improved by 50% relative to CK group; as can be seen from fig. 2F, gu Weijun is effective in improving the yield of the first harvested fruits; as can be seen from FIG. 2G, the average yield of individual tomato fruits of the 250 μm Gu Weijun element treated group increased by 13.5% and the average yield of individual tomato fruits of the 500 μm Gu Weijun element treated group increased by 44.4% relative to the final individual fruit yield of the CK group.
According to the experimental result, gu Weijun element can obviously control plant type and promote the growth of plant seedling height and stem thickness; the plant height and stem thickness can be effectively increased by adopting the concentration Gu Weijun element with 250 mu M, and meanwhile, the Gu Weijun element has obvious effects of promoting flowers and retaining flowers, promoting early fruiting and fruit swelling, wherein the concentration Gu Weijun element solution with 250 mu M can achieve ideal effects of promoting flowers and retaining flowers and promoting early fruiting, and the Gu Weijun element with high content of 500 mu M has obvious effect of promoting the final fruit yield.
Example 3 Regulation of Capsici fructus seed growth by Gu Weijun element
Experimental materials: pepper seeds.
Gu Weijun: 10ppm/30ppm/50ppm concentration.
The experimental method comprises the following steps: soaking Capsici fructus seed in Gu Weijun element solution with different concentrations, placing the seeds with different soaking times in wet culture dish, and selecting 50 granules for each treatmentSeed, ddH 2 O is a Control (CK) and bud length is recorded after 14 days.
Experimental results: as shown in table 1, the seed soaking time was short (within 2 hours), and the Gu Weijun element solution at 50ppm concentration was most favorable for seed growth; and the concentration of the Gu Weijun element solution with the concentration of 10ppm to 30ppm is better than that of the Gu Weijun element solution with the concentration of 50ppm within the seed soaking time of 6 to 12 hours, the seed development can be effectively promoted by combining the treatment time and the seed development condition, wherein the concentration of the Gu Weijun element solution with the concentration of 30ppm to 50ppm has the optimal seed development promoting effect, and the concentration of the Gu Weijun element solution with the concentration of 30ppm is 50ppm.
Table 1 Effect of Gu Weijun element on pepper seed bud Length
Example 4 Regulation of eggplant seed growth by Gu Weijun
Experimental materials: eggplant seeds.
Gu Weijun: 10ppm/30ppm/50ppm concentration.
The experimental method comprises the following steps: soaking eggplant seeds in Gu Weijun element water solutions with different concentrations, placing the seeds with different soaking times in a wet culture dish, recording bud length and fresh weight of the seeds after 14 days, and selecting 50 seeds as parallel samples in ddH (direct seeding) each group of experiments 2 O is a blank group (CK).
Experimental results: as shown in table 2, a Gu Weijun element solution at a concentration of 30ppm was most favorable for seed growth in a short time (within 2 hours); the Gu Weijun element solution with the concentration of 50ppm has better seed soaking effect than the Gu Weijun element solution with the concentration of 10ppm-30ppm in 12 hours, the comprehensive treatment time and the seed development condition can effectively promote the seed development by the Gu Weijun element with the concentration of 10ppm-50ppm, wherein the Gu Weijun element solution with the concentration of 30ppm has the optimal seed development promoting effect, and the next 10ppm.
Table 2 Effect of Gu Weijun on eggplant seed bud Length
The fresh weight test results of the seeds are shown in Table 3, the fresh weight difference of the seeds treated by Gu Weijun elements with different concentrations is obvious under different treatment time, the seed treatment time is less than 6 hours, and Gu Weijun elements with the concentration of 10ppm are most beneficial to the increase of the fresh weight of the seeds; the seed treatment time is between 12 and 24 hours, and 50ppm of Gu Weijun element is most favorable for increasing the fresh weight of the seeds.
Table 3 influence of Gu Weijun on fresh weight of eggplant seeds
Example 5 modulation of wheat seed growth by Gu Weijun element coating
Experimental materials: wheat seeds.
Gu Weijun: 2g of wetting agent, 12g of dispersing agent, 16g of thickening agent, 4g of film forming agent, 26g of antifreezing agent, 4g of pH regulator, 2g of Gu Weijun element and 134g of deionized water are prepared into the seed coating agent with the content of 1 weight percent.
The experimental method comprises the following steps: based on 100kg of seeds, 10g, 50g, 200g, 500g and 2000g of seed coating agents are respectively added into 5 groups of wheat seeds by a seed dressing mode, and the wheat seeds treated by the seed coating agent without the oryzanol are blank control groups (CK), wherein the number of the seeds in each group is 50. The treated seeds are placed in a seedling tray, and after 14 days of cultivation under the conditions of 20 ℃ and 85% RH, the average fresh weight of the seedlings is counted.
Experimental results: as shown in FIG. 3, gu Weijun has remarkable promotion effect on improving the fresh weight of young plants, and the effect difference of Gu Weijun elements with different dosages is remarkable, and the dosage of Gu Weijun elements is not more than 0.02% and the optimal dosage is 0.0001-0.005% based on the total mass of seeds.
Example 6 Regulation of growth of Rice seeds by Gu Weijun element coating
Experimental materials: a rice seed.
Gu Weijun: 2g of wetting agent, 12g of dispersing agent, 16g of thickening agent, 4g of film forming agent, 26g of antifreezing agent, 4g of pH regulator, 2g of Gu Weijun element and 134g of deionized water are prepared into the seed coating agent with the content of 1 weight percent.
The experimental method comprises the following steps: based on 100kg of seeds, 10g, 50g, 200g and 500g of seed coating agents are respectively added into 5 groups of rice seeds by a seed dressing mode, and the rice seeds treated by the seed coating agent without the oryzanol are blank control groups (CK), wherein the number of the seeds in each group is 50. The treated seeds are placed in a seedling tray, and after 14 days of cultivation under the conditions of 28 ℃ and 90% RH, the average root length of the seedlings is counted.
Experimental results: as shown in FIG. 4, gu Weijun has remarkable promotion effect on the growth of seed roots, and the effect difference of Gu Weijun elements with different dosages is remarkable, and the optimal dosage of Gu Weijun elements is 0.0001-0.005% based on the total mass of seeds.
Example 7 Regulation of maize seed growth by Gu Weijun element coating
Experimental materials: corn seeds.
Gu Weijun: 2g of wetting agent, 12g of dispersing agent, 16g of thickening agent, 4g of film forming agent, 26g of antifreezing agent, 4g of pH regulator, 2g of Gu Weijun element and 134g of deionized water are prepared into the seed coating agent with the content of 1 weight percent.
The experimental method comprises the following steps: 100kg of seeds are counted, 100g, 200g and 500g of seed coating agents are respectively added into 3 groups of corn seeds by a seed dressing mode, the corn seeds treated by the seed coating agents without the oryzanol are blank control groups (CK), the treated seeds are placed in a seedling raising tray, the quantity of each group of seeds is 50, the seeds are cultivated under the conditions of 26 ℃ and 85% RH, and the germination vigor and the germination rate of the seeds are respectively counted on the third day and the seventh day.
Experimental results: as can be seen from FIG. 5, gu Weijun can significantly promote germination vigor and germination rate of seeds, and is helpful for centralized emergence of seeds, saving management cost of breeding and seedling, and the dosage of Gu Weijun is not higher than 0.02% and the optimal dosage is 0.0001% -0.01% based on the total mass of seeds.
Example 8 Regulation of Rice seed growth by Gu Weijun element
Experimental materials: a rice seed.
Gu Weijun: a 50ppm concentration solution.
The experimental method comprises the following steps: soaking rice seeds in Gu Weijun aqueous solution with ddH 2 O is a blank group (CK), seeds are moved into seedling trays after 12 hours and divided into 10 groups, 20 seeds of each group are cultivated under the conditions of 28 ℃ and 90% RH, the root length, the plant height and the root length of one group of seedlings are randomly recorded every day from the 18 th day, when the average plant height of the group of seedlings is more than or equal to 14cm, the average root length is more than or equal to 12cm and the average stem thickness is more than or equal to 4mm, the seedlings are considered to reach the transplanting period (transplanting period), the related data of the same-period CK seedlings are recorded, and the experimental results are shown in table 4.
Table 4 Gu Weijun Regulation effects of element on Rice seeds
| Treatment of | Height of plant (cm) | Root length (cm) | Stem thickness (mm) |
| Control | 14.2 | 8.9 | 3.62 |
| Gu Weijun element | 14.2 | 12 | 4.08 |
| Increase rate (%) | — | 34.8 | 12.7 |
Experimental results: as can be seen from Table 4, gu Weijun can accelerate the growth of seedlings and help to shorten the transplanting time of rice, thereby saving management cost and early transplanting.
Example 9: regulation of red grape fruit growth by Gu Weijun
Experimental materials: red grape.
Gu Weijun: 50ppm/70ppm concentration.
The experimental method comprises the following steps: gu Weijun element solutions with different concentrations are sprayed to the flower ears in the sequence of the red flower, the consumption of each flower ear is about 10mL, and ddH is used 2 O is a blank control group (CK), and 5 flowers are selected in each group of experiments. The average length and the number of the fruit grains of the first side branch fruit cluster are counted in the swelling period.
Experimental results: as can be seen from FIG. 6, gu Weijun has the effect of promoting fruit growth and increasing fruit setting rate, wherein Gu Weijun element at 70ppm concentration is optimal.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (15)
1. Use of Gu Weijun in improving plant leaf quality comprising at least one of leaf weight and leaf number.
2. The use according to claim 1, wherein the Gu Weijun element is present in a concentration of 10-100ppm.
3. The application of Gu Weijun in plant flower-promoting and flower-preserving, wherein the flower-promoting and flower-preserving comprises at least one of early flowering, reduced flower drop and improved flower yield.
4. The use according to claim 3, wherein the Gu Weijun element concentration is not less than 100 μm.
5. The application of Gu Weijun in plant type control, wherein the plant type control comprises at least one of plant development regulation, plant height increase and stem thickness increase.
6. The use according to claim 5, wherein the concentration of Gu Weijun is not less than 250 μm.
7. Gu Weijun is used for promoting seed sprout growth, increasing fresh weight of seed, increasing fresh weight of seedling, promoting seed root growth, increasing germination vigor, increasing seed germination rate and shortening transplanting time.
8. The use according to claim 7, wherein the Gu Weijun element is used in an amount of not more than 0.02% by weight of the total mass of the seed in the use for increasing the germination vigor and/or germination rate of the seed.
9. The use according to any one of claims 7 or 8, wherein the Gu Weijun element is used in an amount of 0.0001% to 0.01% by total mass of the seed.
10. The use according to claim 7, wherein the concentration of Gu Weijun is 10-100ppm in the use to promote the growth of seed sprouts or to promote the growth of seed roots.
11. The use according to claim 7, wherein the concentration of Gu Weijun is 10-100ppm in the application of increasing the fresh weight of seeds.
12. The use according to claim 7, wherein Gu Weijun is used in an amount of not more than 0.005% by total mass of seeds in the application for increasing seedling weight and/or promoting seed root growth.
13. Gu Weijun in the application of plant fruit retention and swelling.
14. A method of Gu Weijun for regulating plant growth or seed development comprising the steps of: gu Weijun is applied to the seeds, plant parts and/or whole plants of the plant.
15. The method of claim 14, wherein the Gu Weijun element is applied by one or more of seed soaking, seed dressing, coating, seed spraying, root dipping, flower dipping, fruit dipping, root irrigation.
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