CN117677702A - 根据寡核苷酸池的基因组装 - Google Patents

根据寡核苷酸池的基因组装 Download PDF

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Publication number
CN117677702A
CN117677702A CN202280048699.1A CN202280048699A CN117677702A CN 117677702 A CN117677702 A CN 117677702A CN 202280048699 A CN202280048699 A CN 202280048699A CN 117677702 A CN117677702 A CN 117677702A
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CN
China
Prior art keywords
sequence
nucleic acid
acid molecules
iisre
adamer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202280048699.1A
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English (en)
Chinese (zh)
Inventor
D·L·斯坦普
S·哈维
N·贝尔
J·艾伦
E·斯塔尔
N·法塔拉
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Kamena Biotechnology Co ltd
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Kamena Biotechnology Co ltd
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Publication date
Application filed by Kamena Biotechnology Co ltd filed Critical Kamena Biotechnology Co ltd
Publication of CN117677702A publication Critical patent/CN117677702A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN202280048699.1A 2021-05-11 2022-05-11 根据寡核苷酸池的基因组装 Pending CN117677702A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163186871P 2021-05-11 2021-05-11
US63/186871 2021-05-11
PCT/US2022/028775 WO2022240991A1 (en) 2021-05-11 2022-05-11 Gene assembly from oligonucleotide pools

Publications (1)

Publication Number Publication Date
CN117677702A true CN117677702A (zh) 2024-03-08

Family

ID=82016194

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280048699.1A Pending CN117677702A (zh) 2021-05-11 2022-05-11 根据寡核苷酸池的基因组装

Country Status (8)

Country Link
US (1) US20240247256A1 (https=)
EP (1) EP4337775A1 (https=)
JP (1) JP2024520927A (https=)
KR (1) KR20240005893A (https=)
CN (1) CN117677702A (https=)
AU (1) AU2022274239A1 (https=)
CA (1) CA3218247A1 (https=)
WO (1) WO2022240991A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025120370A1 (en) * 2023-12-08 2025-06-12 Camena Bioscience Limited Compositions and methods for solution-phase, phosphoramidite-free synthesis of nucleic acids

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3737755B1 (en) * 2018-01-12 2024-08-28 Camena Bioscience Limited Method for template-free geometric enzymatic nucleic acid synthesis
WO2020150143A2 (en) * 2019-01-14 2020-07-23 Camena Bioscience Limited Compositions and methods for template-free geometric enzymatic nucleic acid synthesis
WO2021055962A1 (en) 2019-09-19 2021-03-25 Camena Bioscience Limited Compositions and methods for template-free double stranded geometric enzymatic nucleic acid synthesis

Also Published As

Publication number Publication date
JP2024520927A (ja) 2024-05-27
CA3218247A1 (en) 2022-11-17
US20240247256A1 (en) 2024-07-25
WO2022240991A1 (en) 2022-11-17
EP4337775A1 (en) 2024-03-20
AU2022274239A1 (en) 2023-11-23
KR20240005893A (ko) 2024-01-12

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