CN117660157B - Low-temperature storage equipment and method for thalli for culturing vibrio parahaemolyticus phage - Google Patents
Low-temperature storage equipment and method for thalli for culturing vibrio parahaemolyticus phage Download PDFInfo
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- CN117660157B CN117660157B CN202410133130.6A CN202410133130A CN117660157B CN 117660157 B CN117660157 B CN 117660157B CN 202410133130 A CN202410133130 A CN 202410133130A CN 117660157 B CN117660157 B CN 117660157B
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- vibrio parahaemolyticus
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- 238000003860 storage Methods 0.000 title claims abstract description 66
- 241000607272 Vibrio parahaemolyticus Species 0.000 title claims abstract description 24
- 241001052560 Thallis Species 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000012258 culturing Methods 0.000 title claims abstract description 10
- 230000007246 mechanism Effects 0.000 claims abstract description 20
- 230000001580 bacterial effect Effects 0.000 claims abstract description 11
- 238000013016 damping Methods 0.000 claims abstract description 5
- 239000003708 ampul Substances 0.000 claims description 21
- 238000007789 sealing Methods 0.000 claims description 19
- 238000003825 pressing Methods 0.000 claims description 10
- 230000006835 compression Effects 0.000 claims description 5
- 238000007906 compression Methods 0.000 claims description 5
- 229920001821 foam rubber Polymers 0.000 claims description 4
- 229920001971 elastomer Polymers 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000008569 process Effects 0.000 description 6
- 230000009471 action Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000008094 contradictory effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 206010047400 Vibrio infections Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of bacterial culture, in particular to bacterial low-temperature storage equipment and method for culturing vibrio parahaemolyticus phage. Comprises a base; the self-locking universal wheel is arranged at four corner positions of the lower end face of the base, a bearing plate is arranged above the base through a plurality of damping springs, a telescopic pipe is arranged between the bearing plate and the base, a box body is arranged on the bearing plate, a conveying chain plate is rotatably arranged in the box body through a roll shaft, a fixing mechanism is arranged on the conveying chain plate, and a picking and placing mechanism is arranged on the front side of the box body. According to the conveying mechanism provided by the invention, the carrying plates provided with the storage boxes can be moved to the feeding port positions one by one through the conveying chain plates so as to carry out taking and placing operations, and when taking and placing operations are carried out each time, only the feeding port at the corresponding position is required to be opened, so that the space communicated with the outside inside of the box body is greatly reduced, the loss of temperature is reduced, and the survival rate and the stability of thalli are ensured.
Description
Technical Field
The invention relates to the technical field of bacterial culture, in particular to bacterial low-temperature storage equipment and method for culturing vibrio parahaemolyticus phage.
Background
Vibrio parahaemolyticus is a gram negative polymorphic bacillus or Vibrio parahaemolyticus, is mainly distributed in seawater and aquatic products, has characteristics of halophilic acid intolerance, and can be used as a candidate medicament of novel antibiotics to develop novel vibrio resisting medicaments, prevent and treat severe marine animal vibriosis and the like due to the characteristics of the vibrio parahaemolyticus bacteriophage such as cracking property, naturalness and the like. In order to maintain the activity and stability of vibrio parahaemolyticus bacterial body, prolong the service life of vibrio parahaemolyticus phage and maintain the phage activity thereof, so as to better perform laboratory research and application, the vibrio parahaemolyticus bacterial body can be placed into a freeze-drying tube or ampoule bottle and then placed into an ultralow temperature refrigerator for storage.
However, the present thalli mainly have the following problems in the low-temperature storage process: the ultralow temperature refrigerator that uses now needs the big of opening the scope when getting to put the thallus, makes the temperature loss waste resource very fast, and causes the inside easy frosting of refrigerator to increased the frequency of refrigerator defrosting clearance, and influenced the homogeneity and the stability of freezing process, thereby indirectly influenced the survival rate and the stability of thallus.
Disclosure of Invention
In view of the above problems, embodiments of the present application provide an application name to solve the above-mentioned improved technical problems.
In order to achieve the above object, the embodiment of the present application provides the following technical solutions: a first aspect of an embodiment of the present application provides a bacterial cell low-temperature storage device for phage culture of Vibrio parahaemolyticus, comprising a base; the self-locking universal wheel is arranged at four corner positions of the lower end face of the base, a bearing plate is arranged above the base through a plurality of damping springs, a telescopic pipe is arranged between the bearing plate and the base, a box body is arranged on the bearing plate, a conveying chain plate is rotatably arranged in the box body through a roll shaft, a fixing mechanism is arranged on the conveying chain plate, and a picking and placing mechanism is arranged on the front side of the box body.
The fixing mechanism comprises a supporting plate, a plurality of groups of supporting groups are uniformly arranged on the outer surface of the conveying chain plate, each group of supporting groups comprises two supporting plates which are in front-back symmetry, a waist-shaped groove is formed in each supporting plate, a bearing plate is arranged between the two supporting plates in the same group, a shaft rod is arranged on the lower end face of each bearing plate through a supporting seat, two ends of each shaft rod rotate to penetrate through the corresponding waist-shaped groove, 匚 templates are uniformly arranged on the upper end face of each bearing plate along the length direction of each bearing plate, guide grooves are symmetrically formed in the front-back inner wall of the box body, two rollers which are in sliding fit with the guide grooves are respectively arranged on the opposite sides of the two 匚 templates on the same bearing plate, a storage box is placed in each 匚 template, and a fixing unit for fixing the storage box is arranged at the position of the left side of each bearing plate, corresponding to the 匚 templates.
As the preferred scheme, fixed unit includes torsion spring pole, the left side setting of accepting the board rotates and installs a torsion spring pole, and two clamp plates are installed to the symmetry around on the torsion spring pole, and the clamp plate is provided with the rubber pad with the terminal surface of one side that the storing box is contradicted, and the connecting rod is all installed at the front and back both ends of a torsion spring pole, is provided with the pedestal between two connecting rods, is provided with restriction clamp plate pivoted locating part on the accepting board.
As the preferred scheme, get and put the mechanism include the closing plate, the left end face upper portion of box and the position of 匚 template one-to-one on the board of accepting of a set of wherein have seted up the pay-off mouth, sliding mounting has the closing plate in the pay-off mouth, along its length direction even slidable mounting have the bracing piece on the closing plate, the bracing piece is the cavity structure, one side that the closing plate was kept away from to the bracing piece is installed jointly and is even the board, the slide is installed through the pressure spring to one side that the linking plate is close to the box, slide and backup pad sliding connection, rectangular channel has evenly been seted up along its circumference on the bracing piece, the T type post is installed to one side that the storing box is close to the closing plate and bracing piece one-to-one correspondence, be provided with in the bracing piece and be used for being connected fixed complex locking piece with T type post.
As the preferred scheme, the locking piece include the push-and-pull rod, the inside slidable mounting of bracing piece has the push-and-pull rod, installs compression spring between push-and-pull rod left end face and the bracing piece inner wall, evenly sets up the spout along its circumference on the right side inner wall of bracing piece, has the stopper through extension spring slidable mounting in the spout, the spout has been seted up at the middle part of one side that the support bar axle center was kept away from to the stopper, the slide with spout one-to-one intercommunication has been seted up to the bracing piece inner wall, slidable mounting has the driver plate in the slide, install the conflict piece of contradicting with the stopper on the driver plate, a bottom plate with push-and-pull rod fixed connection is installed jointly to the left end of driver plate.
As the preferred scheme, the locating part includes the limiting plate, and two clamp plates of same group are provided with the limiting plate in opposite sides, and the limiting plate rotates through No. two torsional spring poles and installs on the board that holds, and the limiting plate is L type structure, and the interior angle position of limiting plate is contradicted with the spliced pole, and the one end that the limiting plate kept away from the spliced pole is high Yu Cheng fishplate bar up end, and the right side of bracing piece is provided with the rectangle piece of contradicting with the limiting plate.
As a preferable scheme, the guide groove comprises two groups of vertical sections which are bilaterally symmetrical, each group of vertical sections comprises two vertical grooves, and the upper ends and the lower ends of the two groups of vertical sections are respectively communicated with the same horizontal section.
As the preferable scheme, the notch for placing the ampoule bottle is evenly arranged on the upper end face of the storage box, and a foam-rubber cushion is arranged in the notch.
The second aspect of the embodiment of the application provides a method for storing thalli for culturing vibrio parahaemolyticus phage at a low temperature, which is completed by adopting a device for storing thalli for culturing vibrio parahaemolyticus phage at a low temperature, and comprises the following steps: s1, manually opening a feeding port, pushing the storage box with the ampoule bottle placed on a bearing plate, and fixing the storage box through a fixing unit.
S2, the conveying chain plate rotates to enable the next carrying plate to rotate to the feeding port, and then the step S1 is repeated to continuously place the storage box filled with the ampoule bottles.
S3, when the ampoule bottle needs to be taken, the supporting rod is pushed to be matched with the T-shaped column, the T-shaped column is fixed through the locking piece, and the supporting rod is pulled to be taken out of the storage box so as to take thalli in the ampoule bottle.
S4, closing the feeding port through a sealing plate after the thalli are taken and placed.
The above technical solutions in the embodiments of the present invention have at least one of the following technical effects: 1. according to the conveying mechanism provided by the invention, the carrying plates provided with the storage boxes can be moved to the feeding port positions one by one through the conveying chain plates so as to carry out taking and placing operations, and when taking and placing operations are carried out each time, only the feeding port at the corresponding position is required to be opened, so that the space communicated with the outside inside of the box body is greatly reduced, the loss of temperature is reduced, the formation of frost is reduced, the influence on the stability of the temperature inside the box body is reduced, and the survival rate and the stability of thalli are ensured.
2. According to the invention, the support rod is pushed to be matched with the T-shaped plate to finish the pulling butt joint of the storage box, and the fixing unit is released to fix the storage box, so that the storage box can synchronously move along with the support rod, the opening times and the opening time of the feeding port are reduced, and the temperature change degree in the box body is further reduced.
3. The limiting unit provided by the invention keeps the pressing plate to press and fix the storage box and the 匚 template to limit the storage box through the limiting piece, so that the stability of the storage box in the moving process is ensured.
Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic perspective view of the present application.
Fig. 2 is an enlarged view of the structure at a in fig. 1.
Fig. 3 is a schematic perspective view of the case of fig. 1 with a part thereof omitted.
Fig. 4 is a schematic perspective view of a part of the fixing mechanism of the present application.
Fig. 5 is a schematic structural view of a fixing unit according to the present application.
Fig. 6 is a cross-sectional view between the limiting plate and the axle post of the present application.
Fig. 7 is a partial sectional view of the pick-and-place mechanism of the present application.
Fig. 8 is an enlarged view of the structure at B in fig. 7.
Fig. 9 is a schematic view of a guide groove structure of the present application.
Reference numerals: 10. a base; 11. self-locking universal wheels; 12. a damping spring; 13. a bearing plate; 14. a telescopic tube; 15. a case; 16. a conveying chain plate; 2. a fixing mechanism; 20. a support plate; 21. a waist-shaped groove; 22. a receiving plate; 23. a shaft lever; 24. 匚 templates; 25. a guide groove; 26. a roller; 27. a storage box; 270. a sponge cushion; 4. a fixing unit; 40. a pressing plate; 41. a connecting rod; 42. a shaft post; 43. a limiting piece; 430. a limiting plate; 431. rectangular blocks; 3. a picking and placing mechanism; 30. a sealing plate; 31. a support rod; 32. a pressure spring; 33. a slide plate; 34. a T-shaped column; 35. a locking member; 350. a push-pull rod; 351. a compression spring; 352. a chute; 353. a tension spring; 354. a limiting block; 355. a slideway; 356. a driving plate; 357. a collision block; 358. a bottom plate.
Detailed Description
In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the invention, whereby the invention is not limited to the specific embodiments disclosed below.
As shown in fig. 1 and 3, a low-temperature bacterial storage device for phage culture of vibrio parahaemolyticus comprises a base 10, wherein a self-locking universal wheel 11 is arranged at four corner positions of the lower end face of the base 10, a bearing plate 13 is arranged above the base 10 through a plurality of damping springs 12, a telescopic pipe 14 is arranged between the bearing plate 13 and the base 10, a box 15 is arranged on the bearing plate 13, a conveying chain plate 16 is rotatably arranged in the box 15 through a roll shaft, a fixing mechanism 2 is arranged on the conveying chain plate 16, and a picking and placing mechanism 3 is arranged on the front side of the box 15.
As shown in fig. 3, fig. 4 and fig. 5, the fixing mechanism 2 includes a supporting plate 20, multiple groups of supporting groups are uniformly arranged on the outer surface of the conveying chain plate 16, each group of supporting groups includes two supporting plates 20 which are symmetrical front and back, a waist-shaped groove 21 is formed in each supporting plate 20, a bearing plate 22 is arranged between the two supporting plates 20 in the same group, a shaft lever 23 is mounted on the lower end surface of each bearing plate 22 through a support, two ends of each shaft lever 23 penetrate through the corresponding waist-shaped groove 21 in a rotating manner, 匚 templates 24 are uniformly mounted on the upper end surface of each bearing plate 22 along the length direction, guide grooves 25 are symmetrically formed in the front and back inner walls of the box 15, two rollers 26 which are in sliding fit with the guide grooves 25 are respectively arranged on opposite sides of two 匚 templates 24 on the same bearing plate 22, a storage box 27 is placed in each 匚 template 24, and a fixing unit 4 for fixing the storage box 27 is arranged at the position corresponding to the 匚 templates on the left side of each bearing plate 22.
As shown in fig. 9, the guiding slot 25 includes two groups of vertical segments that are symmetrical left and right, each group of vertical segments includes two vertical slots, and the upper and lower ends of the two groups of vertical segments are respectively connected with the same horizontal segment.
As shown in fig. 2, fig. 5, fig. 7 and fig. 8, the picking and placing mechanism 3 comprises a sealing plate 30, a feeding port is formed in the upper portion of the left end face of the box body 15 at a position corresponding to 匚 templates 24 on one group of carrying plates 22, the sealing plate 30 is slidably mounted in the feeding port, a supporting rod 31 is uniformly slidably mounted on the sealing plate 30 along the length direction of the sealing plate 30, the supporting rod 31 is of a cavity structure, one side of the supporting rod 31, far away from the sealing plate 30, is jointly provided with a connecting plate, one side of the connecting plate, close to the box body 15, is provided with a sliding plate 33 through a pressure spring 32, the sliding plate 33 is slidably connected with the supporting plate 20, rectangular grooves are uniformly formed in the circumferential direction of the supporting rod 31, one side of the storage box 27, close to the sealing plate 30, is provided with T-shaped columns 34 in one-to-one correspondence with the supporting rod 31, and locking pieces 35 for fixedly matching with the T-shaped columns 34 are arranged in the supporting rod 31.
In the concrete working process, the sealing plate 30 is pulled manually through the supporting rod 31 to separate the sealing plate 30 from the feeding port, then the storage box 27 with the ampoule bottle (with thalli filled in) is pushed onto the bearing plate 22 manually, the storage box 27 is abutted against the 匚 template 24, the storage box 27 is fixed through the fixing unit 4, the feeding port is sealed through the sealing plate 30 after the fixing is finished, an external motor for driving a roll shaft is arranged on the box body 15, the external motor operates to drive the conveying chain plate 16 to rotate through the roll shaft, the conveying chain plate 16 rotates to drive the connecting plate 22 to rotate, the next bearing plate 22 rotates to the feeding port position to continuously place the storage box 27, in addition, when the bearing plate 22 moves to the corner of the conveying chain plate 16, the rolling shaft 26 moves from the vertical section of the guide groove 25 to the horizontal section, at the moment, the supporting plate 20 is positioned at the joint of the vertical section and the circular arc section of the conveying chain plate 16, along with the rotation of the conveying chain plate 16, the supporting plate 20 starts to perform circular motion, in the process of rotating the supporting plate 20, under the condition that the roller 26 is limited by the horizontal section of the guide groove 25, the shaft rod 23 slides relative to the waist-shaped groove 21, the 匚 template 24, the bearing plate 22 and the roller 26 integrally perform linear motion along the horizontal section of the guide groove 25, the 匚 template 24 is always upwards, when the supporting plate 20 rotates along the circular arc section of the conveying chain plate 16, namely, the supporting plate 20 rotates to a horizontal state again, the roller 26 is positioned at the joint of the horizontal section of the guide groove 25 and the other vertical section, and then along with the rotation of the conveying chain plate 16, the supporting plate 20 keeps the horizontal state to perform vertical motion, and the roller 26 can drive the bearing plate 22 to synchronously move along the vertical section under the limit of the vertical section of the guide groove 25, and in the whole process, the surface of the bearing plate 22 provided with the 匚 template 24 is always upwards; when the thalli need to be taken out, the supporting rod 31 is pushed to be matched with the T-shaped column 34, the limit of the fixing unit 4 on the storage box 27 is released, the sliding plate 33 is pulled to fix the T-shaped column 34 through the locking piece 35, the sliding plate 33 is pulled, the supporting rod 31 is pulled to enable the sealing plate 30 to be separated from the feeding port, the corresponding storage box 27 is moved out, thalli can be taken at the moment, and the storage box 27 is manually placed on the corresponding receiving plate 22 after taking is completed and is fixed through the fixing unit 4.
As shown in fig. 5, the upper end surface of the storage box 27 is uniformly provided with a notch for placing an ampoule bottle, and a foam-rubber cushion 270 is arranged in the notch; in particular, the foam-rubber cushion 270 can ensure that the ampoule is tightly placed in the notch, so that the stability of the ampoule can be ensured and the ampoule can be protected from the outside when the conveying chain plate 16 drives the carrying plate 22 with the storage box 27.
As shown in fig. 4 and 5, the fixing unit 4 includes a torsion spring rod, a first torsion spring rod is rotatably mounted on the left side of the receiving plate 22, two pressing plates 40 are symmetrically mounted on the first torsion spring rod, a rubber pad is disposed on an end face of one side of the pressing plate 40, which abuts against the storage box 27, connecting rods 41 are mounted on the front end and the rear end of the first torsion spring rod, a shaft column 42 is disposed between the two connecting rods 41, and a limiting member 43 for limiting the rotation of the pressing plate 40 is disposed on the receiving plate 22.
As shown in fig. 5, 6 and 7, the limiting member 43 includes a limiting plate 430, the opposite sides of the two pressing plates 40 in the same group are provided with the limiting plate 430, the limiting plate 430 is rotatably mounted on the bearing plate 22 through a second torsion spring rod, the limiting plate 430 is in an L-shaped structure, the inner angle position of the limiting plate 430 is in contact with the axle post 42, one end of the limiting plate 430, which is far away from the axle post 42, is higher than the upper end surface of the bearing plate 22, and the right side of the supporting rod 31 is provided with a rectangular block 431 in contact with the limiting plate 430.
When the ampoule bottle is taken, the rectangular block 431 is abutted against the limiting plate 430 to enable the limiting plate 430 to rotate, the limiting plate 430 rotates away from the storage box 27 under the action of the first torsion spring rod, and the storage box 27 can be pulled out of the feeding port together with the supporting rod 31 at the moment to take the thalli.
As shown in fig. 7 and 8, the locking member 35 includes a push-pull rod 350, the push-pull rod 350 is slidably mounted in the support rod 31, a compression spring 351 is mounted between the left end surface of the push-pull rod 350 and the inner wall of the support rod 31, a sliding groove 352 is uniformly formed in the right inner wall of the support rod 31 along the circumferential direction of the right inner wall, a limiting block 354 is slidably mounted in the sliding groove 352 through a tension spring 353, a chute is formed in the middle of one side of the limiting block 354 away from the axis of the support rod 31, a sliding track 355 in one-to-one correspondence with the sliding groove 352 is formed in the inner wall of the support rod 31, a driving plate 356 is slidably mounted in the sliding track 355, a collision block 357 in collision with the limiting block 354 is mounted on the driving plate 356, and a bottom plate 358 fixedly connected with the push-pull rod 350 is commonly mounted at the left end of the driving plate 356.
Specifically, during operation, the supporting rod 31 is pushed to approach the T-shaped column 34, so that the inner cavity of the supporting rod 31 covers the T-shaped column 34, then the sliding plate 33 is pulled to move towards the connecting plate, the sliding plate 33 moves to pull the driving plate 356 away from the storage box 27 through the push-pull rod 350 and the bottom plate 358, the driving plate 356 can squeeze the limiting block 354 through the abutting block 357, the limiting block 354 can move towards the axis of the T-shaped column 34 under the squeezing of the abutting block 357, the vertical section of the T-shaped column 34 is clamped between the limiting block 354 and the end part of the push-pull rod 350, then the sliding plate 33 is pulled, and the supporting rod 31 is pulled, so that the storage box 27 and the supporting rod 31 move out of the feeding port together for taking the thalli. After the thalli is taken, the storage box 27 is pushed to move to the corresponding carrying plate 22 through the supporting rod 31, then the sliding plate 33 is loosened, the push-pull rod 350 moves towards the T-shaped column 34 under the combined action of the compression spring 351 and the pressure spring 32, the push-pull rod 350 enables the abutting block 357 to be far away from the limiting block 354 through the bottom plate 358 and the driving plate 356, the limiting block 354 is far away from the T-shaped column 34 under the action of the tension spring 353, the limitation on the T-shaped column 34 is relieved, the sealing plate 30 is moved out of the feeding port, the storage box 27 is fixed through the fixing unit 4, and then the feeding port is sealed through the sealing plate 30.
In addition, the invention also provides a low-temperature bacterial cell storage device for culturing the vibrio parahaemolyticus phage, which is completed by adopting the low-temperature bacterial cell storage device for culturing the vibrio parahaemolyticus phage, and comprises the following steps: s1, the sealing plate 30 is manually moved through the supporting rod 31 to be separated from the feeding port, so that the feeding port is opened, then the storage box 27 with the ampoule bottle is manually pushed onto the bearing plate 22 through the feeding port, the storage box 27 is fixed through the fixing unit 4, and then the feeding port is sealed through the sealing plate 30.
S2, the conveying chain plate 16 rotates to enable the next receiving plate 22 to rotate to the feeding port position, and then the step S1 is repeated to continuously place the storage box 27 filled with ampoule bottles.
S3, when the ampoule bottle needs to be taken, the supporting rod 31 is pushed manually, the T-shaped column 34 is wrapped in the inner cavity of the supporting rod 31, then the locking piece 35 is pulled manually through the sliding plate 33, the T-shaped column 34 is fixed in the supporting rod 31 by the locking piece 35, then the sliding plate 33 is pulled, the connecting plate is pulled to move outwards, the sealing plate 30 is separated from the feeding port, the feeding port is opened at the moment, the supporting rod 31 is pulled continuously until the storage box 27 is moved out from the feeding port, and then the storage box 27 is placed back to the original position after the ampoule bottle with the required quantity is taken.
S4, closing the feeding port through the sealing plate 30 after the thalli are taken and placed.
In the description of the present invention, it should be understood that the azimuth or positional relationships indicated by the azimuth terms such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal", and "top, bottom", etc., are generally based on the azimuth or positional relationships shown in the drawings, merely to facilitate description of the present invention and simplify the description, and these azimuth terms do not indicate and imply that the apparatus or elements referred to must have a specific azimuth or be constructed and operated in a specific azimuth, and thus should not be construed as limiting the scope of protection of the present invention; the orientation word "inner and outer" refers to inner and outer relative to the contour of the respective component itself.
Furthermore, the terms "first," "second," "first," and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "first", "second", "first", "second" may include at least one such feature, either explicitly or implicitly. In the description of the present invention, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "connected," "mounted," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
The embodiments of the present invention are all preferred embodiments of the present invention, and are not intended to limit the scope of the present invention; all equivalent changes in structure, shape and principle of the invention should be covered in the scope of protection of the invention.
Claims (7)
1. A low-temperature storage device for bacterial cells for phage culture of Vibrio parahaemolyticus comprises a base (10); the method is characterized in that: four corner positions of the lower end face of the base (10) are provided with a self-locking universal wheel (11), a bearing plate (13) is arranged above the base (10) through a plurality of damping springs (12), a telescopic pipe (14) is arranged between the bearing plate (13) and the base (10), a box body (15) is arranged on the bearing plate (13), a conveying chain plate (16) is rotatably arranged in the box body (15) through a roll shaft, a fixing mechanism (2) is arranged on the conveying chain plate (16), and a picking and placing mechanism (3) is arranged on the front side of the box body (15); wherein:
The fixing mechanism (2) comprises a supporting plate (20), a plurality of groups of supporting groups are uniformly arranged on the outer surface of the conveying chain plate (16), each group of supporting groups comprises two supporting plates (20) which are in front-back symmetry, a waist-shaped groove (21) is formed in each supporting plate (20), a bearing plate (22) is arranged between the two supporting plates (20) of the same group, a shaft rod (23) is arranged on the lower end face of each bearing plate (22) through a support, two ends of each shaft rod (23) penetrate through the corresponding waist-shaped groove (21) in a rotating mode, 匚 templates (24) are uniformly arranged on the upper end face of each bearing plate (22) along the length direction of each bearing plate, guide grooves (25) are symmetrically formed in the front inner wall and the rear inner wall of each box body (15), two rolling shafts (26) which are in sliding fit with the guide grooves (25) are formed in the opposite sides of the two front-rear 匚 templates (24) on the same bearing plate (22), a storage box (27) is placed in each 匚 template (24), and a fixing unit (4) for fixing the storage box (27) is arranged at the position corresponding to the left side of each bearing plate (22);
Get and put mechanism (3) include closing plate (30), the left end face upper portion of box (15) has offered the pay-off mouth with the position of 匚 template (24) one-to-one on the board of accepting of wherein a set of (22), slidable mounting has closing plate (30) in the pay-off mouth, evenly slidable mounting has bracing piece (31) along its length direction on closing plate (30), bracing piece (31) are cavity structure, one side that closing plate (30) was kept away from to bracing piece (31) is installed jointly and is even the board, even board is close to one side of box (15) and is installed slide (33) through pressure spring (32), slide (33) and backup pad (20) sliding connection, rectangular channel has evenly been offered along its circumference on bracing piece (31), one side that storing box (27) is close to closing plate (30) is installed T type post (34) with bracing piece (31) one-to-one, be provided with in bracing piece (31) and be used for being connected fixed complex locking piece (35).
2. The low-temperature storage device for phage culture of Vibrio parahaemolyticus according to claim 1, wherein: the fixing unit (4) comprises torsion spring rods, a torsion spring rod is rotatably arranged on the left side of the bearing plate (22), two pressing plates (40) are symmetrically arranged on the torsion spring rod in a front-back mode, rubber pads are arranged on one side end face of the pressing plates (40) which are abutted to the storage box (27), connecting rods (41) are arranged at the front end and the rear end of the torsion spring rod, a shaft column (42) is arranged between the two connecting rods (41), and limiting pieces (43) for limiting the rotation of the pressing plates (40) are arranged on the bearing plate (22).
3. The low-temperature storage device for phage culture of Vibrio parahaemolyticus according to claim 1, wherein: the locking piece (35) include push-and-pull rod (350), push-and-pull rod (350) is installed to bracing piece (31) inside slidable mounting, install compression spring (351) between push-and-pull rod (350) left end face and bracing piece (31) inner wall, spout (352) have evenly been seted up along its circumference to the right side inner wall of bracing piece (31), have stopper (354) through extension spring (353) slidable mounting in spout (352), the chute has been seted up at one side middle part that the axle center was kept away from to stopper (354), slide (355) with spout (352) one-to-one intercommunication have been seted up to bracing piece (31) inner wall, sliding mounting has driving plate (356) in slide (355), install on driving plate (356) with the conflict piece (357) of stopper (354) conflict, bottom plate (358) of being fixedly connected with push-and-pull rod (350) are installed jointly to the left end of driving plate (356).
4. The low-temperature storage device for phage culture of Vibrio parahaemolyticus according to claim 2, wherein: the limiting piece (43) comprises a limiting plate (430), wherein the limiting plate (430) is arranged on the opposite sides of the two pressing plates (40) in the same group, the limiting plate (430) is rotatably arranged on the bearing plate (22) through a second torsion spring rod, the limiting plate (430) is of an L-shaped structure, the inner angle position of the limiting plate (430) is in conflict with the axle column (42), one end, far away from the axle column (42), of the limiting plate (430) is higher than the upper end face of the bearing plate (22), and a rectangular block (431) in conflict with the limiting plate (430) is arranged on the right side of the supporting rod (31).
5. The low-temperature storage device for phage culture of Vibrio parahaemolyticus according to claim 1, wherein: the guide groove (25) comprises two groups of vertical sections which are bilaterally symmetrical, each group of vertical sections comprises two vertical grooves, and the upper ends and the lower ends of the two groups of vertical parts are respectively communicated with the same horizontal section.
6. The low-temperature storage device for phage culture of Vibrio parahaemolyticus according to claim 1, wherein: the upper end face of the storage box (27) is uniformly provided with a notch for placing an ampoule bottle, and a foam-rubber cushion (270) is arranged in the notch.
7. A low-temperature storage method of thalli for culturing vibrio parahaemolyticus phage is characterized in that: the method for culturing the vibrio parahaemolyticus phage using the low temperature storage device according to claim 1, comprising the following steps:
S1, manually opening a feeding port, pushing a storage box (27) with ampoule bottles placed on a bearing plate (22), and fixing the storage box (27) through a fixing unit (4);
S2, rotating a conveying chain plate (16) to enable the next receiving plate (22) to rotate to the feeding port, and then repeating the step S1 to continuously place a storage box (27) filled with ampoule bottles;
S3, when the ampoule bottle needs to be taken out, pushing the supporting rod (31) to be matched with the T-shaped column (34), fixing the T-shaped column (34) through the locking piece (35), and pulling the supporting rod (31) to bring out the storage box (27) so as to take out thalli in the ampoule bottle;
s4, closing the feeding port through a sealing plate (30) after the thalli are taken and placed.
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| CN106995803A (en) * | 2017-01-23 | 2017-08-01 | 集美大学 | One plant is used to prevent and treat the sick bacteriophage of prawn vibrio parahaemolytious and its propagation method |
| CN116639294A (en) * | 2023-05-26 | 2023-08-25 | 内蒙古中润生物科技有限公司 | Method for preparing corn distillers' grains by utilizing probiotics fermentation |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101381676A (en) * | 2008-10-23 | 2009-03-11 | 上海交通大学 | Cryopreservation in situ apparatus and method for adherent cell three-dimensional culture |
| CN102524131A (en) * | 2012-01-20 | 2012-07-04 | 广东海洋大学 | Method for enriching vibrio phage and biologically preventing host bacteria |
| WO2014003538A1 (en) * | 2012-06-26 | 2014-01-03 | Biovalence Sdn. Bhd. | Rapid specific pathogen free animal |
| CN106995803A (en) * | 2017-01-23 | 2017-08-01 | 集美大学 | One plant is used to prevent and treat the sick bacteriophage of prawn vibrio parahaemolytious and its propagation method |
| CN116639294A (en) * | 2023-05-26 | 2023-08-25 | 内蒙古中润生物科技有限公司 | Method for preparing corn distillers' grains by utilizing probiotics fermentation |
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