CN117647657B - Test paper type assay device applied to staphylococcus aureus food - Google Patents

Test paper type assay device applied to staphylococcus aureus food Download PDF

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Publication number
CN117647657B
CN117647657B CN202410124761.1A CN202410124761A CN117647657B CN 117647657 B CN117647657 B CN 117647657B CN 202410124761 A CN202410124761 A CN 202410124761A CN 117647657 B CN117647657 B CN 117647657B
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China
Prior art keywords
movable plate
test paper
movable
groove
fixedly connected
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CN202410124761.1A
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Chinese (zh)
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CN117647657A (en
Inventor
于江
吴家强
丁罗刚
李贵阳
张玉玉
任素芳
刘飞
陈智
曾昊
徐敏丽
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Qingdao Insight Exbio Co ltd
Institute Animal Science and Veterinary Medicine of Shandong AAS
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Qingdao Insight Exbio Co ltd
Institute Animal Science and Veterinary Medicine of Shandong AAS
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Priority to CN202410124761.1A priority Critical patent/CN117647657B/en
Publication of CN117647657A publication Critical patent/CN117647657A/en
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Abstract

The invention discloses a test paper type testing device applied to staphylococcus aureus food, which comprises a base, wherein an installation frame is fixed at the upper end of the base, two electric push rods are installed on the inner top wall of the installation frame, the telescopic ends of the two electric push rods face downwards and are fixedly connected with the same first movable plate, a second movable plate is arranged below the first movable plate, two connecting mechanisms connected with the first movable plate are arranged at the upper end of the second movable plate, a reagent adding mechanism is arranged between the first movable plate and the second movable plate, a placing table is arranged at the upper end of the base, and a first placing groove and a second placing groove which are arranged left and right are arranged on the side wall of the placing table. The invention can realize continuous automatic operation of feeding the test paper tape and adding the reagent, does not need manual operation, saves time and labor, and greatly improves the efficiency of staphylococcus aureus food assay.

Description

Test paper type assay device applied to staphylococcus aureus food
Technical Field
The invention relates to the technical field of assay devices, in particular to a test paper type assay device applied to staphylococcus aureus food.
Background
Staphylococcus aureus is the most common food-borne pathogenic bacteria in suppurative infection of human beings, has high and low temperature resistance, high permeation resistance and strong survivability, is commonly in the environment, can accurately combine with a target gene according to the specific primer designed by the femA gene of staphylococcus aureus at present, establishes a staphylococcus aureus nucleic acid detection method, and realizes result visualization, high-speed, high-efficiency, high-sensitivity and high-specificity detection and high-efficiency test paper type assay device by adding SYBRGreenI.
The publication No. CN216117347U is a Chinese patent for a test paper type testing device applied to staphylococcus aureus food, and discloses a test paper type testing device applied to staphylococcus aureus food, which comprises a test table arranged on the ground, wherein a placing table is arranged in the middle of the top surface of the test table, a paper box with good sealing is arranged on the right side of the top of the test table, a dustbin is arranged at the bottom of the test table, and meanwhile, the dustbin is positioned under the placing table. The feeding of test paper, the adding operation of reagent still need the manual work in this patent, and the process is comparatively loaded down with trivial details, consequently can't realize the continuous automatic operation of feeding of test paper, adding of reagent, wastes time and energy, influences detection efficiency, in the great detection operation of work load, the problem of inefficiency is particularly prominent.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a test paper type testing device applied to staphylococcus aureus food.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides a be applied to test paper formula chemical examination device of staphylococcus aureus food, includes the base, the upper end of base is fixed with the mounting bracket, install two electric putter on the inner roof of mounting bracket, two electric putter's flexible end is same first movable plate down and fixedly connected with, the below of first movable plate is equipped with the second movable plate, the upper end of second movable plate is equipped with two coupling mechanism who is connected with first movable plate, be equipped with reagent adding mechanism between first movable plate and the second movable plate, the upper end of base is equipped with the stand, be equipped with first standing groove, the second standing groove that sets up about on the lateral wall of stand, all be equipped with the opening on the inner wall of first standing groove, second standing groove, the inside of first standing groove is equipped with test paper mechanism, the inside of standing table is equipped with the mounting groove that is located between first standing groove and the second standing groove, the inside of mounting groove is equipped with actuating mechanism, the upper end of standing table is equipped with two hold-down mechanisms, the inside of standing table is equipped with the movable groove that is close to the second standing groove, the inside of cutting off is equipped with movable groove.
As a further improvement of the invention, the connecting mechanism comprises a guide post fixedly connected to the upper end of the second moving plate, the guide post penetrates through the first moving plate and is in sliding connection with the first moving plate, a first spring is sleeved on the guide post, one end of the first spring is connected to the first moving plate, and the other end of the first spring is connected to the second moving plate.
As a further improvement of the invention, the reagent adding mechanism comprises a reagent tank arranged at the upper end of the first moving plate, four piston cylinders arranged in an array are fixed at the upper end of the second moving plate, connecting pipes are connected to the side walls of the reagent tank and connected with the piston cylinders, piston rods are inserted into the upper ends of the four piston cylinders, a liquid outlet pipe is connected to the side walls of the piston cylinders, and the liquid outlet pipe penetrates through the second moving plate downwards.
As a further improvement of the invention, the test paper mechanism comprises an inserting shaft fixedly connected to the inner side wall of the first placing groove, a reel is sleeved on the inserting shaft, a test paper tape is wound on the reel, the test paper tape comprises a plastic base tape, test paper sheets are arranged on the tape surface of the plastic base tape, and two rows of tooth holes positioned on two sides of the test paper sheets are arranged on the plastic base tape.
As a further improvement of the invention, the driving mechanism comprises a rotating shaft arranged in the mounting groove, two ends of the rotating shaft are rotatably connected to the inner side wall of the mounting groove, two rotating wheels are fixedly sleeved on the rotating shaft, six shifting teeth which are arranged at equal intervals along the circumferential direction are fixedly arranged on the wheel wall of the rotating wheel, the shifting teeth correspond to tooth holes, a gear is sleeved on the rotating shaft through a one-way bearing, and a rack matched with the gear is fixedly connected to the lower end of the second moving plate.
As a further improvement of the invention, the compressing mechanism comprises an L-shaped plate fixedly connected to the upper end of the placing table, a telescopic rod is fixed on the inner top wall of the L-shaped plate, the telescopic end of the telescopic rod faces downwards and is fixedly connected with a wheel frame, a compressing wheel is rotatably arranged on the inner side of the wheel frame, a second spring is sleeved on the telescopic rod, one end of the second spring is connected with the L-shaped plate, and the other end of the second spring is connected with the wheel frame.
As a further improvement of the invention, the cutting mechanism comprises a sealing plate arranged in the movable groove, the upper end of the sealing plate is fixedly connected with a cutter, the upper end of the sealing plate is fixedly connected with two movable rods, the two movable rods are symmetrically arranged on two sides of the cutter, the movable rods penetrate through the inner top wall of the movable groove and are in sliding connection with the placing table, the movable rods penetrate through the second movable plate and are in sliding connection with the second movable plate, the upper end of the movable rods is fixedly connected with a first limiting block, the movable rods are fixedly sleeved with a second limiting block, the first limiting block and the second limiting block are respectively positioned on the upper side and the lower side of the second movable plate, rubber rings are fixedly embedded on the placing table, and the rubber rings are sleeved on the movable rods.
As a further improvement of the invention, a collecting box is arranged inside the second placing groove.
As a further improvement of the invention, a baffle is hinged on the side wall of the placing table.
The invention has the beneficial effects that:
1. through setting up coupling mechanism, reagent adding mechanism, when electric putter extension drives first movable plate and descends, can drive the first synchronous motion of second movable plate through coupling mechanism, stop the back when the second movable plate receives the stop of placing the platform, first movable plate continues to move down and extrudees the piston rod, can extrude the inside reagent of piston cylinder through the drain pipe through the piston rod, realize that the reagent is automatic to be added to the test paper piece on, need not manual operation and add the reagent, labour saving and time saving, and reagent addition is more accurate.
2. Through setting up reagent mechanism, actuating mechanism, after accomplishing reagent and adding and chemical examination, electric putter shrink can drive the second and remove the board and reciprocate, and then the second removes the board and reciprocate and to drive the rack, can drive actuating mechanism action through the rack, drives the reagent area through actuating mechanism and removes the pay-off, and then can realize the reagent area automatic feeding of next section to place the platform top, need not the manual work and operates, labour saving and time saving improves the efficiency of chemical examination.
3. Through setting up the second standing groove, collect box, shutdown mechanism, when the second movable plate is in the highest position, the second movable plate can provide holding power to first stopper for the shrouding can carry out the shutoff to the opening on the second standing groove, and then realizes the isolation to the second standing groove, and is sanitary and safe more, can collect the plastic baseband after using, test paper piece through collecting the box and deposit, and when the second movable plate moves down and be in the lowest position, the second movable plate pushes down the second stopper, can drive the shrouding through the movable rod and move down, and then can not influence the test paper area and get into the second standing groove.
4. Through setting up hold-down mechanism, provide down force to the pinch roller through the second spring, compress tightly the plastic baseband through the pinch roller, and then can guarantee the test paper tape and place the stability of platform, and then can guarantee the stability of chemical examination process.
The invention can realize continuous automatic operation of feeding the test paper tape and adding the reagent, does not need manual operation, saves time and labor, and greatly improves the efficiency of staphylococcus aureus food assay.
Drawings
FIG. 1 is a schematic diagram of a test paper type assay device for Staphylococcus aureus food according to the present invention;
FIG. 2 is an enlarged view of FIG. 1 at A;
FIG. 3 is an enlarged view of FIG. 1 at B;
fig. 4 is a schematic perspective view of a cutting mechanism of a test paper type testing device for staphylococcus aureus food according to the present invention;
fig. 5 is a schematic diagram of a top view structure of a spindle, a rotating wheel, a poking tooth and a gear of a test paper type testing device for staphylococcus aureus food according to the present invention;
fig. 6 is a schematic diagram showing a part of the structure of a reagent strip of a test paper type testing device for staphylococcus aureus food according to the present invention.
In the figure: 1 base, 2 mounting bracket, 3 electric putter, 4 reagent jar, 5 first movable plate, 6 first spring, 7 guide post, 8 second movable plate, 9 connecting pipe, 10 piston rod, 11 piston tube, 12 drain pipe, 13 rack, 14 first stopper, 15 movable rod, 16 second stopper, 17 place the platform, 18 first standing groove, 19 cartridge axle, 20 reel, 21 mounting groove, 22 runner, 23 plectrum, 24 pivot, 25 collection box, 26 baffle, 27 second standing groove, 28 telescopic link, 29 second spring, 30 wheel carrier, 31 pinch roller, 32L template, 33 movable groove, 34 cutter, 35 shrouding, 36 rubber circle, 37 gear, 38 plastic baseband, 39 test paper piece, 40 tooth aperture.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
Fig. 1-6, a test paper type testing device for staphylococcus aureus food, comprising a base 1, wherein the upper end of the base 1 is fixed with a mounting frame 2, two electric push rods 3 are installed on the inner top wall of the mounting frame 2, the telescopic ends of the two electric push rods 3 are downward and fixedly connected with a first movable plate 5, a second movable plate 8 is arranged below the first movable plate 5, the upper end of the second movable plate 8 is provided with two connecting mechanisms connected with the first movable plate 5, a reagent adding mechanism is arranged between the first movable plate 5 and the second movable plate 8, the upper end of the base 1 is provided with a placing table 17, a first placing groove 18 and a second placing groove 27 which are arranged left and right are arranged on the side wall of the placing table 17, a collecting box 25 is arranged in the second placing groove 27, and used plastic base bands 38 and test paper sheets 39 can be collected and stored through the collecting box 25, the baffle 26 is hinged on the side wall of the placing table 17, the baffle 26 which can be opened and closed is arranged, when the baffle 26 is closed, the first placing groove 18 and the second placing groove 27 can be plugged, when the baffle 26 is opened, the operation on the inside of the first placing groove 18 and the inside of the second placing groove 27 can be conveniently carried out, the inner walls of the first placing groove 18 and the second placing groove 27 are respectively provided with a through hole, the inside of the first placing groove 18 is provided with a test paper mechanism, the test paper mechanism comprises an inserting shaft 19 fixedly connected on the inner side wall of the first placing groove 18, a reel 20 is sleeved on the inserting shaft 19, a test paper tape is wound on the reel 20, the test paper tape comprises a plastic base band 38, test paper sheets 39 are arranged on the band surface of the plastic base band 38, two rows of tooth holes 40 positioned on two sides of the test paper sheets 39 are arranged on the plastic base band 38, the inside of the placing table 17 is provided with a mounting groove 21 positioned between the first placing groove 18 and the second placing groove 27, the inside of the mounting groove 21 is provided with a driving mechanism, the upper end of the placing table 17 is provided with two pressing mechanisms, the inside of the placing table 17 is provided with a movable groove 33 close to the second placing groove 27, and the inside of the movable groove 33 is provided with a cutting mechanism.
In the invention, the connecting mechanism comprises a guide post 7 fixedly connected to the upper end of a second movable plate 8, the guide post 7 penetrates through a first movable plate 5, the guide post 7 is in sliding connection with the first movable plate 5, a first spring 6 is sleeved on the guide post 7, one end of the first spring 6 is connected to the first movable plate 5, and the other end of the first spring 6 is connected to the second movable plate 8.
The specific reagent adding mechanism comprises a reagent tank 4 arranged at the upper end of a first movable plate 5, four piston cylinders 11 which are arranged in a fixed manner are fixed at the upper end of a second movable plate 8, connecting pipes 9 are connected to the side walls of the reagent tank 4, the connecting pipes 9 are connected with the piston cylinders 11, piston rods 10 are inserted into the upper ends of the four piston cylinders 11, liquid outlet pipes 12 are connected to the side walls of the piston cylinders 11, the liquid outlet pipes 12 penetrate through the second movable plate 8 downwards, and the four liquid outlet pipes 12 can simultaneously drip four test paper sheets 39.
The specific actuating mechanism is including setting up the inside pivot 24 of mounting groove 21, the both ends of pivot 24 are all rotated and are connected on the inside wall of mounting groove 21, fixed sleeve has two runners 22 on the pivot 24, be fixed with six of equidistant setting in circumference on the wheel wall of runner 22 and dial tooth 23 and tooth hole 40 and correspond, dial tooth 23 can block in tooth hole 40, and then can stir reagent area through dialling tooth 23 and remove the material loading, be equipped with gear 37 through the one-way bearing cover on the pivot 24, set up one-way bearing, make gear 37 anticlockwise rotation time, can not drive pivot 24 rotation, and gear 37 clockwise rotation time can drive pivot 24 rotation, the lower extreme fixedly connected with of second movable plate 8 and gear 37 complex rack 13, and then can not drive pivot 24 rotation when making rack 13 move down, can drive pivot 24 rotation when rack 13 moves up.
The specific hold-down mechanism comprises an L-shaped plate 32 fixedly connected to the upper end of the placement table 17, a telescopic rod 28 is fixed on the inner top wall of the L-shaped plate 32, the telescopic end of the telescopic rod 28 faces downwards and is fixedly connected with a wheel frame 30, a hold-down wheel 31 is rotatably arranged on the inner side of the wheel frame 30, a second spring 29 is sleeved on the telescopic rod 28, one end of the second spring 29 is connected with the L-shaped plate 32, and the other end of the second spring 29 is connected with the wheel frame 30.
The concrete cutting mechanism includes setting up the shrouding 35 in movable groove 33 inside, the upper end fixedly connected with cutter 34 of shrouding 35, the upper end fixedly connected with two movable rods 15 of shrouding 35, two movable rods 15 symmetry set up in the both sides of cutter 34, movable rod 15 runs through the interior roof of movable groove 33 and with place platform 17 sliding connection, movable rod 15 runs through second movable plate 8 and with second movable plate 8 sliding connection, the upper end fixedly connected with first stopper 14 of movable rod 15, the fixed second stopper 16 that has cup jointed on the movable rod 15, first stopper 14, second stopper 16 are located the upper and lower both sides of second movable plate 8 respectively, place fixedly on the platform 17 and inlay and be equipped with rubber circle 36, rubber circle 36 suit is on movable rod 15, set up rubber circle 36, can utilize the better frictional force of rubber circle 36, keep the stability of movable rod 15 state, make movable rod 15 only can move when the atress is driven.
When the test paper is used, food to be tested is placed on the test paper sheet 39 on the test paper belt, the electric push rod 3 is started to extend, when the electric push rod 3 extends to drive the first moving plate 5 to descend, the second moving plate 8 can be driven to synchronously move through the guide post 7, after the second moving plate 8 stops moving due to the blocking of the placing table 17, the first moving plate 5 continuously moves downwards and extrudes the piston rod 10, so that the reagent in the piston cylinder 11 can be extruded through the liquid outlet pipe 12 through the piston rod 10, the reagent can be automatically added to the test paper sheet 39, and judgment is carried out according to the color reaction of the test paper;
when the second moving plate 8 moves downwards to be at the lowest position, the second moving plate 8 presses down the second limiting block 16, the sealing plate 35 can be driven by the movable rod 15 to move downwards, so that the test paper tape can not be influenced to enter the second placing groove 27, after detection, the electric push rod 3 is started to shrink, the elastic force of the first spring 6 acts on the first moving plate 5, so that the first moving plate 5 moves upwards, the piston rod 10 is driven by the first moving plate 5 to move upwards, the reagent in the reagent tank 4 can be drawn into the fine piston cylinder 11 through the connecting pipe 9, the next reagent adding requirement is met, the electric push rod 3 shrinks and can drive the second moving plate 8 to move upwards, the second moving plate 8 can further drive the rack 13 to move upwards, the gear 37 is driven by the rack 13 to rotate clockwise, the rotating shaft 24 and the rotating wheel 22 are driven by the gear 37, the reagent tape can be driven by the poking tooth 23 to move and feed, and further the reagent tape of the next section can be automatically fed to the place above the placing table 17 without manual operation;
when the second movable plate 8 is at the highest position, the second movable plate 8 can provide supporting force for the first limiting block 14, so that the sealing plate 35 can seal the through hole on the second placing groove 27, the second placing groove 27 is isolated, sanitation and safety are further achieved, meanwhile, the sealing plate 35 can drive the cutter 34 to cut off the plastic base band 38, and the used plastic base band 38 and test paper 39 can be collected and stored through the collecting box 25.
The present invention is not limited to the above-mentioned embodiments, and any person skilled in the art, based on the technical solution of the present invention and the inventive concept thereof, can be replaced or changed within the scope of the present invention.

Claims (8)

1. The utility model provides a be applied to test paper formula chemical examination device of staphylococcus aureus food, includes base (1), its characterized in that, the upper end of base (1) is fixed with mounting bracket (2), install two electric putter (3) on the interior roof of mounting bracket (2), two electric putter (3) flexible end down and fixedly connected with same first movable plate (5), the below of first movable plate (5) is equipped with second movable plate (8), the upper end of second movable plate (8) is equipped with two coupling mechanism who is connected with first movable plate (5), be equipped with reagent addition mechanism between first movable plate (5) and second movable plate (8), the upper end of base (1) is equipped with standing board (17), be equipped with first standing groove (18) that set up about the lateral wall of standing board (17), second standing groove (27), all be equipped with the opening on the inner wall of first standing groove (18) and second standing groove (27), be equipped with two coupling mechanism that are connected with first movable plate (5), be equipped with between two inside mounting groove (21) and the inside of standing board (17) are equipped with the inside-out mechanism (21) that is equipped with, inside-in the standing groove (21) is equipped with on the standing board (17), the inside of placing platform (17) is equipped with movable groove (33) that is close to second standing groove (27), the inside of movable groove (33) is equipped with shutdown mechanism, shutdown mechanism is including setting up in inside shrouding (35) of movable groove (33), the upper end fixedly connected with cutter (34) of shrouding (35), the upper end fixedly connected with of shrouding (35) two movable rods (15), two movable rods (15) symmetry sets up in the both sides of cutter (34), movable rods (15) run through the interior roof of movable groove (33) and with place platform (17) sliding connection, movable rods (15) run through second movable plate (8) and with second movable plate (8) sliding connection, the upper end fixedly connected with first stopper (14) of movable rods (15), second stopper (16) have been cup jointed on movable rods (15), first stopper (14), second stopper (16) are located the upper and lower both sides of second movable plate (8) respectively, place platform (17) and inlay on fixed circle (36) rubber sleeve-mounted rubber (36).
2. The test paper type assay device for staphylococcus aureus food according to claim 1, wherein the connecting mechanism comprises a guide post (7) fixedly connected to the upper end of the second moving plate (8), the guide post (7) penetrates through the first moving plate (5), the guide post (7) is slidably connected with the first moving plate (5), a first spring (6) is sleeved on the guide post (7), one end of the first spring (6) is connected to the first moving plate (5), and the other end of the first spring (6) is connected to the second moving plate (8).
3. The test paper type assay device applied to staphylococcus aureus food according to claim 1, wherein the reagent adding mechanism comprises a reagent tank (4) arranged at the upper end of a first movable plate (5), four piston cylinders (11) arranged in a row are fixed at the upper end of a second movable plate (8), connecting pipes (9) are connected to the side walls of the reagent tank (4), the connecting pipes (9) are connected with the piston cylinders (11), piston rods (10) are inserted into the upper ends of the four piston cylinders (11), a liquid outlet pipe (12) is connected to the side walls of the piston cylinders (11), and the liquid outlet pipe (12) penetrates through the second movable plate (8) downwards.
4. The test paper type testing device for staphylococcus aureus food according to claim 1, wherein the test paper mechanism comprises an inserting shaft (19) fixedly connected to the inner side wall of the first placing groove (18), a reel (20) is sleeved on the inserting shaft (19), a test paper tape is wound on the reel (20), the test paper tape comprises a plastic base tape (38), test paper sheets (39) are arranged on the tape surface of the plastic base tape (38), and two rows of tooth holes (40) positioned on two sides of the test paper sheets (39) are formed in the plastic base tape (38).
5. The test paper type assay device applied to staphylococcus aureus food according to claim 4, wherein the driving mechanism comprises a rotating shaft (24) arranged in the mounting groove (21), two ends of the rotating shaft (24) are rotatably connected to the inner side wall of the mounting groove (21), two rotating wheels (22) are fixedly sleeved on the rotating shaft (24), six poking teeth (23) arranged at equal intervals along the circumferential direction are fixed on the wheel wall of the rotating wheel (22), the poking teeth (23) correspond to tooth holes (40), a gear (37) is sleeved on the rotating shaft (24) through a one-way bearing, and a rack (13) matched with the gear (37) is fixedly connected to the lower end of the second moving plate (8).
6. The test paper type testing device for staphylococcus aureus food according to claim 1, wherein the pressing mechanism comprises an L-shaped plate (32) fixedly connected to the upper end of the placing table (17), a telescopic rod (28) is fixed on the inner top wall of the L-shaped plate (32), the telescopic end of the telescopic rod (28) faces downwards and is fixedly connected with a wheel frame (30), a pressing wheel (31) is rotatably arranged on the inner side of the wheel frame (30), a second spring (29) is sleeved on the telescopic rod (28), one end of the second spring (29) is connected with the L-shaped plate (32), and the other end of the second spring (29) is connected with the wheel frame (30).
7. A dipstick assay device for s.aureus food according to claim 1 characterised in that the second reservoir (27) is internally provided with a collection box (25).
8. A dipstick assay device for s.aureus food according to claim 1 characterised in that a baffle (26) is hinged to the side wall of the platform (17).
CN202410124761.1A 2024-01-30 2024-01-30 Test paper type assay device applied to staphylococcus aureus food Active CN117647657B (en)

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CN214703662U (en) * 2021-05-18 2021-11-12 江西惠肽生物科技有限公司 Test paper sampling device for detecting prealbumin
CN216117347U (en) * 2021-11-03 2022-03-22 云南商测质量检验技术服务有限公司 Test paper type assay device applied to staphylococcus aureus food
CN216771528U (en) * 2021-11-23 2022-06-17 咸宁市荣大防腐技术有限责任公司 PH test paper suit for safe use
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CN115524461A (en) * 2022-10-23 2022-12-27 濮明祥 Mildew detection equipment and method for preparing thick broad-bean sauce
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