CN117563034A

CN117563034A - Use of an exudate-activated battery for the preparation of an energy-supplying fabric as an electroactive wound dressing

Info

Publication number: CN117563034A
Application number: CN202311551943.9A
Authority: CN
Inventors: 武华君; 康燕堂; 鲁志松; 徐立群
Original assignee: Southwest University
Current assignee: Southwest University
Priority date: 2023-12-21
Filing date: 2023-12-21
Publication date: 2024-02-20

Abstract

The invention discloses an application of an exudate activated battery in preparing an energy supply fabric as an electric active wound dressing, wherein cotton yarn is used as a battery support, N-dimethylaminoethyl methacrylate is grafted onto the cotton yarn to obtain modified cotton yarn, which is called CPY, CYP is wrapped on magnesium wires, then carbon yarn is twisted outside to prepare the exudate activated battery, which is called CYPEAB, the CYPEAB is connected in parallel, and the electric energy supply fabric is obtained by weaving through a traditional weaving technology, which is called EFD.

Description

Use of an exudate-activated battery for the preparation of an energy-supplying fabric as an electroactive wound dressing

Technical Field

The invention belongs to the field of biological materials, and relates to an application of an exudate activated battery in preparing an energy supply fabric as an electroactive wound dressing.

Background

The skin acts as a protective barrier between the internal organs of the human body and the environment, and its function is affected after injury. Wound repair is a complex dynamic process involving four phases of hemostasis, inflammation, proliferation and remodeling. This involves the migration and action of multiple cell types that must be closely coordinated to effectively repair damaged tissue. There is evidence that the transition from the inflammatory phase to the proliferative phase is critical in the healing process. This is because inflammation serves as the main defense of the human body against pathogenic wound attack, helping to clear dead tissue. Provides for tissue regeneration. However, chronic inflammation produces a large amount of exudates due to capillary permeability. Excessive exudates can interfere with cell proliferation, disrupt extracellular matrix, provide favorable survival conditions for bacteria and pathogens, exacerbate inflammation, cause vicious circle, and ultimately lead to delayed wound healing, affecting wound healing. Currently, the main ways to enhance healing for the different phases of wound repair include management of exudates, prevention of bacterial infection and promotion of active repair of wounds.

At present, effective management of excess exudates is beneficial in maintaining a suitably moist environment at the wound site. If hydrophilic wound dressing (such as hydrogel, foam, sponge) is used to facilitate the absorption of excessive exudates, but bacteria internalized in the dressing remain viable after the exudates are absorbed, biofilm may form on the dressing surface, and the wound still faces the risk of bacterial infection. Bacterial infection can destroy the microenvironment of the wound, promote chronic inflammation and delay healing. The antimicrobial properties of the dressing are critical to effective repair of wound tissue. For bacterial infections, modification of wound dressings, the introduction of antimicrobial agents (such as poly (N, N-dimethylaminoethyl methacrylate)) is an important means of combating bacterial infections. Wound healing is aided by covering the wound site to protect the wound from bacteria or external injury. But these dressings lack the ability to actively promote wound closure and healing. Since endogenous Electric Fields (EF) are widely present in cells and tissues of microorganisms, plants and animals, and are important signals that dominate cell behavior, in recent years, electrical Stimulation (ES) has been applied in wound therapy to achieve active repair of wounds.

Disclosure of Invention

In view of the above, it is an object of the present invention to provide the use of an exudate activated battery for the preparation of an electroactive wound dressing energy delivery fabric. The invention specifically provides the following technical scheme:

the application of the exudate activated battery in preparing an energy supply fabric as an electric active wound dressing takes cotton yarn as a battery support, the modified cotton yarn is obtained by grafting N, N-dimethylaminoethyl methacrylate onto the cotton yarn, which is called CPY, the CYP is wrapped on magnesium wires, then the carbon yarn is twisted outside to prepare the exudate activated battery, which is called CYPEAB, the CYPEAB is connected in parallel, the energy supply fabric is obtained by braiding through the traditional braiding technology, which is called CYPEFD, the CYPEFD can be used as the electric active wound dressing, and the electric active wound dressing is activated to supply power after absorbing wound exudate, so that the exudate activated wound dressing has the functions of exudate management, anti-infection and wound repair promotion.

Further, the using method of the electroactive wound dressing comprises the following steps: when activated by wound exudates, power is supplied to the wound. Ions in the exudates act as electrolytes to power the activation of the exudates battery and provide electrical stimulation therapy to the wound.

Further, the grafting refers to grafting an initiator on the surface of the cotton yarn, initiating an atom transfer radical polymerization reaction of N, N-dimethylaminoethyl methacrylate on the surface, and grafting the initiator on the surface of the cotton yarn.

Further, the atom transfer radical polymerization reaction uses CuBr/PMDETA as a catalytic system, and uses 2-bromoisobutyryl bromide in an aqueous medium system to prepare the cotton macroinitiator.

Further, the preparation steps of the CYPEFD are as follows:

sequentially placing cotton yarn into sodium hydroxide and sodium carbonate, heating, boiling, washing, and drying in an oven;

2) Dichloromethane and triethylamine were added to a round bottom flaskCooling in ice bath to 0-5deg.C, soaking the cotton yarn in step 1) in round bottom flask, and adding N ₂ After flushing, slowly dripping BiBB, reacting for 2-3 hours at 0-5 ℃, reacting for 18-24 hours at room temperature, washing with dichloromethane, distilled water and ethanol in sequence, and drying in a vacuum oven to obtain the cotton thread macromolecular initiator;

3) Adding the water-ethanol mixture into a round flask, adding DMAEMA, cuBr and PMDETA, soaking the cotton macromolecular initiator obtained in the step 2) into the flask, and exhausting air in the flask; after reacting for 12-18 hours, ultrasonically washing with distilled water and ethanol to obtain modified cotton yarn, namely CPY;

4) Wrapping the CPY obtained in the step 3) on magnesium wires, and then twisting the carbon yarns outside for integration to obtain an effusion activated battery called CYPEAB;

5) The CYPEAB of step 4) is connected in series and in parallel, and is woven by conventional weaving techniques to obtain an energy supplying fabric, called CYPEFD.

Further, in the step 1), the cotton yarn is washed by heating and boiling for 2 times each for 30 minutes in 10g/L sodium hydroxide and 10g/L sodium carbonate in sequence.

Further, the reaction temperature in the step 3) is 50-60 ℃.

Further, the CYPEAB obtained in the step 4) is 5-7 cm in length.

Further, the number of the CYPEAB connected in series and parallel in the step 5) is 5.

The invention has the beneficial effects that:

an electroactive dressing based on a exudate activated battery (CYPEAB) takes cotton threads as a substrate, has good liquid absorption, can absorb excessive exudate at a wound by improving the liquid absorption performance after being modified by PDMAEMA, takes the exudate as electrolyte, and can be directly activated to supply the required Electric Stimulation (ES) effect for the wound. The anode product magnesium hydroxide of the cell was co-antimicrobial with PDMAEMA during this process. In the study, the CYPEAB electroactive dressing exhibited good antibacterial properties and exudate absorption. And the raw yarn (CY) was also woven into a exudate activated battery (CYEAB), the antimicrobial properties of CYEAB and cypea were compared, and cypea electrically stimulated cell growth and biocompatibility were analyzed. The result shows that CYPEAB is a multifunctional dressing with the functions of resisting bacteria, managing exudates, promoting active repair of wounds and the like. The dressing can effectively manage wound exudates, has excellent antibacterial performance, and can simulate the endogenous Electric Field (EF) in a wound to guide migration of cells required by wound regeneration such as macrophages, fibroblasts and the like to an injured area so as to accelerate wound healing. The method has the advantages of simple operation, mild reaction conditions, safety, environmental protection, good stability and the like.

Drawings

In order to make the objects, technical solutions and advantageous effects of the present invention clearer, the present invention provides the following drawings:

FIG. 1 is a schematic representation of the preparation of a CYPEAB-powered electroactive wound dressing.

Fig. 2 is an electrical property of a simulated body fluid after activating CYPEAB.

FIG. 3 is a graph of the number of bacteria surviving on the surface of E.coli and Staphylococcus aureus after contact with CYPEAB before and after activation.

FIG. 4 experiments on the bactericidal mechanism of E.coli and Staphylococcus aureus by CYPEAB before and after activation.

FIG. 5 leakage of proteins from E.coli and Staphylococcus aureus by CYPEAB before and after activation.

FIG. 6 is a graph of CYPEAB-induced electric field strength versus fibroblast behavior in vitro.

Figure 7 is a graph of an evaluation of a rat subcutaneous infection model for the promotion of wound repair by a CYPEAB electroactive dressing.

Figure 8 is a graph of evaluation of in vivo antimicrobial in a CYPEAB electroactive dressing from a rat subcutaneous infection model.

Description of the embodiments

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

The CYPEAB powered electroactive wound dressing is prepared by the reaction shown in figure 1, wherein a cotton yarn modified by poly (N, N-dimethylaminoethyl methacrylate) (PDMAEMA) is used as a liquid absorbing substrate of a battery, the Cotton Yarn (CYP) modified by PDMAEMA is wrapped on magnesium wires, then carbon yarns are twisted outside to prepare an exudate activated battery (CYPEAB), and a plurality of CYPEAB connected in parallel are woven into the electroactive wound dressing CYPEFD.

Examples

The preparation of the exudate activated battery CYPEAB comprises the following specific contents:

1) Sequentially placing cotton yarn into 10g/L sodium hydroxide and 10g/L sodium carbonate, heating, boiling, washing, and drying in an oven at 60 ℃;

2) 120 mL dichloromethane and 2.4 mL triethylamine are added into a 250 mL round-bottomed flask, ice bath is cooled to 0-5 ℃, and after the cotton yarn described in step 1) is soaked in the round-bottomed flask; by N ₂ After flushing, 2 mL of 2-bromoisobutyryl bromide (BiBB) is slowly dripped;

3) Reacting the material obtained in the step 2) at 0-5 ℃ for 2 hours, reacting at room temperature for 24 hours, washing with dichloromethane, distilled water and ethanol in sequence, and drying in a vacuum oven at 60 ℃;

4) Adding 40 ml water-ethanol mixture into a 100 mL round flask, adding 1.6 mL N, N-dimethylaminoethyl methacrylate (PDMAEMA), 11.5 mg CuBr and 34 mu L PMDETA, soaking the materials in the step 3) into the flask, and exhausting air in the flask; after reacting for 12 hours, washing cotton yarn by distilled water and ethanol ultrasonic wave;

5) Wrapping the cotton yarn in the step 4) of 5 cm on magnesium wires, and then twisting the carbon yarn outside with a fixed number of turns of 3 turns per 1cm for integration to obtain CYPEAB.

Examples

Preparation of a CYPEAB-powered electroactive wound dressing (CYPEFD), the specific contents are as follows:

2) 120 mL dichloromethane and 2.4 mL triethylamine were added to a 250 mL round bottom flask and cooled to 0-5 ℃ in an ice bath to cool the steps1) The cotton yarn is soaked in a round-bottom flask; by N ₂ After flushing, 2 mL of 2-bromoisobutyryl bromide (BiBB) is slowly dripped;

5) Wrapping the cotton yarn in the step 4) of 5 cm on magnesium wires, and then twisting the carbon yarn outside at a fixed circle number of 3 circles per 1cm for integration to obtain CYPEAB;

6) The energy supplying fabric, namely the electroactive wound dressing (cytofd), is obtained by braiding 5 cytoabs described in step 5) in parallel by conventional braiding techniques.

The electrical properties of the CYPEAB prepared in example 1 were evaluated using an electrochemical workstation as follows:

the main components of wound exudates are plasma, interstitial fluid, and contains a large amount of electrolytes (Na ⁺ 、K ⁺ Etc.). Can be used as CYPEAB environment-friendly electrolyte to activate CYPEAB for energy supply. The two poles of CYPEAB are respectively connected, and after the starting and the electrifying, the electrical performance test is carried out, and the test result is shown in figure 2.

As can be seen in fig. 2a, a5 cm long CYPEAB can be activated by 1 μl of simulated body fluid.

As can be seen from fig. 2b-c, after addition of saturated volumes of simulated body fluid, the maximum open circuit voltage and short circuit current of the cell reached 1.8V and 2mA, respectively;

fig. 2d shows that two cytoabs can be used to illuminate a small LED bulb with an operating voltage of 1.5V after being connected in series.

We speculate that CYPEAB, after absorption of the Simulated Body Fluid (SBF), can be rapidly activated, triggering the cathodic reduction and anodic oxidation reactions. Electrons generated at the anode flow through an external circuit to the cathode, while ions move between the electrodes through the electrolyte to maintain charge balance and chemical reaction progress, thereby forming a stable current output.

Taken together, the results of electrical performance testing using Simulated Body Fluid (SBF) to simulate wound exudates to explore CYEAB, which acts as an energy delivery fabric that can be successfully activated after SBF absorption.

The antibacterial properties of the surfaces of the materials before and after energy supply of the CYPEAB prepared in example 1 were evaluated by a plating counting method, and the specific contents are as follows:

culturing bacteria: the frozen tube containing the strain is taken out from the refrigerator at the temperature of minus 20 ℃ to be melted, then inoculated into TSB culture medium and shake-cultured at the temperature of 37 ℃ for standby.

2. The specific steps are (the equipment and the liquid used in the following steps are sterilized in advance):

bacterial suspensions were diluted to a concentration of 1X 10 with PBS buffer ⁷ cells/mL；

Placing cotton yarns with the specification of 2 cm CY and CYP and the specification of discharging after the two yarns are assembled into batteries respectively in 24 pore plates, adding 1 mL bacterial solution into each pore respectively, and culturing for 4 hours in a constant-temperature incubator at 37 ℃;

sucking out the bacterial solution, adding 1 mL PBS buffer solution along the hole wall to clean the surface of the cotton yarn, and repeating the steps for three times;

adding 3 mL of PBS buffer solution into a 15 mL centrifuge tube, placing the washed cotton yarn into the centrifuge tube, and performing ultrasonic treatment for 7 minutes to strip bacteria adhered to the surface;

adding 900 mu L of PBS buffer solution into a 1.5 mL centrifuge tube, sucking 100 mu L of the ultrasonic-stripped bacterial solution, shaking and uniformly mixing, and carrying out gradient dilution;

100. Mu.L of the gradient diluted solution was pipetted onto TSB agar plates, spread with a spreading bar until the bacterial solution was completely absorbed, and the agar plates were incubated at 37℃for 24 hours and counted by photographing.

FIG. 3 is E.coli（E·coli）And staphylococcus aureus（S·aureus）Graph of bacterial count surviving on the surface after exposure to CYEAB before and after activation.

As can be seen from FIG. 3, E.coli is to be used（E·coli）And staphylococcus aureus（S·aureus）After co-cultivation with CY prior to discharge, a significant reduction in CYP surface bacterial colonization compared to CY was observed. However, PDMAEMA alone does not eliminate all bacteria. CYP was prepared as CYPEAB, the cell was activated with simulated body fluid, and after discharge, co-cultured with bacteria, and almost no bacterial colonies were observed on the plates. The excellent antimicrobial properties are attributed to the simulated body fluid entering the yarn, flowing rapidly along the yarn, connecting the cathode and anode, activating the cell.

The bacterial mechanism of CYPEAB prepared in example 1 was evaluated by hydrolysis of o-nitrobenzene-beta-D-galactopyranoside (ONPG), and the specific contents are as follows:

The specific steps are (the equipment and the liquid used in the following steps are sterilized in advance):

The cotton yarn after the battery is assembled and discharged by the original cotton yarn CY and the PDMAEMA modified cotton yarn CYP with the specification of 2 cm or the two yarns is respectively placed in a 24-hole plate, 500 mu L of bacterial solution is respectively added into each hole, and the cotton yarn is cultured for 4 hours in a constant-temperature incubator at 37 ℃;

mu.L of ONPG solution (0.75M, naH) was added to each well ₂ PO ₄ Buffer solution, pH 7.0), and reacting at room temperature for 1-3 hours;

the absorbance of the yellow supernatant in each well was recorded at 405nm by an enzymatic device.

As can be seen from FIG. 4, when the cell membrane of the bacteria is damaged, ONPG is more likely to enter the inside of the bacteria and react with beta-D-galactosylglycase (beta-Gal) in the bacteria to produce yellow o-nitrophenol.

CY group and CY group before activation discharge, CY group is largeEnterobacteria @E·coli）And staphylococcus aureus @ sS·aureus）The membrane permeability of (a) is significantly increased compared with the CY group, and the increase effect is more obvious after activation.

The bactericidal mechanism of CYPEAB in example 1 against bacteria was evaluated using a biquinolinecarboxylic acid (BCA) protein assay kit, as follows:

1. culturing bacteria: the frozen tube containing the strain is taken out from the refrigerator at the temperature of minus 20 ℃ to be melted, then inoculated into TSB culture medium and shake-cultured at the temperature of 37 ℃ for standby.

1) Bacterial suspensions were diluted to a concentration of 1X 10 with PBS buffer ⁷ cells/mL；

2) Respectively assembling the original cotton yarn with the specification of 2 cm, the PDMAEMA modified cotton yarn or the two yarns into a battery, discharging the battery, placing the battery into a 24-hole plate, respectively adding 500 mu L of bacterial solution into each hole, and culturing for 4 hours in a constant-temperature incubator at 37 ℃;

3) 1 mL of BCA working solution was added to each well, and the wells were left at 60℃for 30 minutes, and absorbance at other wavelengths between A562, or 540 and 595nm, was measured using a microplate reader.

As can be seen from FIG. 5, E.coli after activation compared to before activationE·coli）And staphylococcus aureus @ sS·aureus）Is significantly increased, indicating that the surface quaternary ammonium groups (NH ²⁺ ) And magnesium hydroxide interact with the bacterial membrane to synergistically destroy the permeability of the bacterial membrane. Bacterial membrane damage can affect the normal function of bacteria by the efflux of some important substances (proteins) inside the bacteria.

The in vitro evaluation of stimulation of cell growth after energization of the cytoab of example 1 was performed using L929 cells, and is specifically described below:

1. l-929 fibroblasts were cultured in Dalberg's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum and 1% penicillin-streptomycin for use. The cell culture conditions were 37 ℃ and 5% oxidative wet incubator.

1) Diluting the L929 cell concentration to 2 ∙ 10-3 cfu/mL with the complete medium;

2) Two parallel titanium wires (15 mm x 0.2 mm) were placed at the bottom of each well of a 24-well cell culture plate, and two perpendicular titanium wires were also placed at the sides of each well to form a T-shaped electrode for ES effect. Inoculating the cells into a 24-well cell culture plate;

3) After 12 hours of incubation, electrodes were connected to both ends of the cytoab to stimulate cells. The stimulation was continued for 72 hours, 2 times per day for 2 hours. Control experiments were performed by placing electrodes in the cell wells without cotton fiber ends;

4) After 12 hours of incubation, the blank cells were incubated for a further 72 hours without any treatment.

FIG. 5 is a graph of in vitro evaluation of CYPEAB-induced electric field strength versus L929 cell behavior: fibroblasts were electrically stimulated with CYPEAB for 3 consecutive days, labeled with Calcein/4', 6-diamidino-2-phenylindole (Calcein-AM/DAPI) for observation under a fluorescence microscope.

As can be seen from fig. 5 a, L929 cells of the electro-stimulated group (With ES) administered through the cypea group showed significant proliferation of cell growth compared to the blank group and the non-electro-stimulated group (Without ES), indicating the exact role of the electric field generated after activation of the cypea by body fluid in cell chemotactic induction, helping migration and proliferation of cells to the wound site, promoting wound repair.

As can be seen in FIG. 6b, the cell counting kit (CCK-8) results are also consistent with the fluorescence image results. Indicating that CYPEAB has low toxicity to organisms.

The wound healing promoting performance of the cytoed of example 2 was evaluated using a rat subcutaneous infection model, and the specific contents are as follows:

1. and respectively manufacturing the original yarn CY and the PDMAEMA modified yarn CYP into an exudate excited battery CYEAB and CYPEAB, and then respectively braiding 5 CYEAB and CYPEAB to obtain the wound dressing CYEFD and CYPEFD.

2. SD rats (200-220 g) were used for the experiment. The experimental part was divided into 2 groups (n = 3), and CYEFD and CYPEFD were applied to the wound sites of rats, respectively.

3. The specific procedures were as follows (all animal experiments were performed according to the guidelines for animal experiments approved by the university of southwest animal use and Care Committee (UCUCUCA))

1) Partially anesthetizing the rat by operation, and shaving and sterilizing the back of the rat;

2) A full thickness circular wound (diameter 1 cm) was formed in the back region of the mouse using a hole cutter (diameter 1 cm), followed by 20. Mu.l of Staphylococcus aureus (1X 10) ⁷ CFU/ml) solution inoculation wound area;

3) The prepared CYEFD and CYPEFD powered electroactive wound dressings (1 cm x 1 cm) were placed over the wound area, respectively, and the dressings were firmly attached to the wound site using commercially available transparent film application (Tegaderm, 3m,1624 WB) films, with the wound covered by Tegaderm film alone, with the blank wound being left unaddressed;

4) Rats were sacrificed 5 d and CYEFD and CYPEFD powered electroactive wound dressings were removed;

5) Detecting immunopathological changes of heart, liver, spleen, lung, kidney and skin of the transplanted rats and healthy rats by adopting an H & E staining method;

6) The variation of collagen deposition in transplanted rats was examined using Masson staining.

From figure 7a it can be seen that the CYPEED group showed a complete epidermis around the tissue compared to the blank and CYEFD groups.

As can be seen from fig. 7b-c, on day 6, the CYPEED group wound contracted to 20.6%, while the blank and CYEFD groups were 41.9% and 34.8%, respectively; by day of treatment, the CYPEED group wounds had been successfully repaired, significantly higher than the blank and CYEFD groups, and the wound sites had grown intact epidermis.

Figure 7d shows that the dual effects of the electrical stimulation and the antimicrobial agent of the CYPEFD group allow it to observe ordered arrangement of high density collagen fibers in the dermis.

Figure 7e shows that the dual effects of the electrical stimulation and the antimicrobial agent of the CYPEFD group caused a 1.4-fold increase in collagen deposition rate in the dermis layer.

As described above, the electro-active dressing cytofd composed of cytoab is capable of repairing an infected wound by inhibiting bacterial growth by PDMAEMA and magnesium hydroxide, and then promoting angiogenesis and tissue regeneration by self-powered treatment of cytofd, thereby achieving excellent healing performance.

The in vivo antibacterial properties of CYPEFD in example 2 were evaluated using a rat subcutaneous infection model, as follows:

3) The prepared CYPEFD powered electroactive wound dressing (1 cm x 1 cm) was placed over the wound area and the dressing was firmly attached to the wound site using a commercially available Tegaderm film, the blank wound was not dressing, and the wound was covered with Tegaderm film alone;

calculating adhesion bacteria on the CYEFD and CYPEFD by adopting a plate method;

rat skin pathology tissue to which the implant was attached was examined using hematoxylin/eosin staining experiments (H & E) and Masson staining experiments (Masson).

Fig. 8 is an evaluation graph of a rat subcutaneous infection model for the promotion of wound repair by a CYPEFD electroactive dressing: after 5 d, the implant was removed and bacteria on the substrate were detected using plating. Appropriate dilutions were performed to count the number of colonies on the surface of the different treatment materials.

As can be seen from fig. 8a, a large number of bacterial aggregates appear on the material surface of the blank, the CYEFD surface bacteria are significantly reduced, while the CYPEFD surface has almost no bacterial residues;

as can be seen from fig. 8b, the survival rate of the bacteria on the surface of the blank group was 99.5%, the survival rate of the bacteria on the surface of CYEFD was 18.7%, and the survival rate of the bacteria on the surface of CYPEFD was only 0.8%.

The above proves that the electro-active dressing based on the synergistic effect of electric stimulation and antibacterial can promote wound repair and resist wound infection.

Finally, it is noted that the above-mentioned preferred embodiments are only intended to illustrate rather than limit the invention, and that, although the invention has been described in detail by means of the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention as defined by the appended claims.

Claims

1. The application of the exudate activated battery in preparing an energy supply fabric as an electric active wound dressing is characterized in that cotton yarn is used as a battery support, N-dimethylaminoethyl methacrylate is grafted on the cotton yarn to obtain modified cotton yarn which is called CPY, CYP is wrapped on magnesium wires, then carbon yarn is twisted outside to prepare the exudate activated battery which is called CYPEAB, the CYPEAB is connected in parallel, the energy supply fabric is obtained by weaving through a traditional weaving technology, the CYPEFD can be used as the electric active wound dressing, and the electric active wound dressing is activated to supply power after absorbing wound exudate and has the functions of exudate management, anti-infection and wound repair promotion.

2. Use of an exudate activated battery for the preparation of an energy-supplying fabric as an electroactive wound dressing, characterized in that the electroactive wound dressing is used in a method of: when activated by wound exudates, power is supplied to the wound. Ions in the exudates act as electrolytes to power the activation of the exudates battery and provide electrical stimulation therapy to the wound.

3. The use of an exudate activated battery as claimed in claim 1 for the preparation of an energy supplying fabric as an electroactive wound dressing, wherein the grafting is to graft an initiator onto the surface of cotton yarn, which initiates an atom transfer radical polymerization of N, N-dimethylaminoethyl methacrylate onto the surface of cotton yarn.

4. Use of an exudate activated battery according to claim 1 for the preparation of an energy supply fabric as an electroactive wound dressing, characterized in that: the atom transfer radical polymerization reaction uses CuBr/PMDETA as a catalytic system, and uses 2-bromo isobutyryl bromide in an aqueous medium system to prepare the cotton macromolecular initiator.

5. Use of an exudate activated battery in the preparation of an energy supplying fabric as an electroactive wound dressing according to claim 1 wherein the EFD is prepared by the steps of:

1) Sequentially placing cotton yarn into sodium hydroxide and sodium carbonate, heating, boiling, washing, and drying in an oven;

2) Adding dichloromethane and triethylamine into a round-bottom flask, cooling to 0-5deg.C in ice bath, soaking cotton yarn in step 1) in the round-bottom flask, and adding N ₂ After flushing, slowly dripping BiBB, reacting for 2-3 hours at 0-5 ℃, reacting for 18-24 hours at room temperature, washing with dichloromethane, distilled water and ethanol in sequence, and drying in a vacuum oven to obtain the cotton thread macromolecular initiator;

3) Adding the water-ethanol mixture into a round flask, adding DMAEMA, cuBr and PMDETA, soaking the cotton macromolecular initiator obtained in the step 2) into the flask, and exhausting air in the flask; after reacting for 12-18 hours, washing with distilled water and ethanol by ultrasonic wave to obtain modified cotton yarn, called CPY;

5) The CYPEAB of step 4) is connected in series and parallel and woven by conventional weaving techniques to obtain an energy supplying fabric, known as EFD.

6. Use of an exudate activated battery according to claim 1 according to claim 4 for the preparation of an energy supply fabric as an electroactive wound dressing, characterized in that: in the step 1), cotton yarn is heated, boiled and washed for 2 times each for 30 minutes in 10g/L sodium hydroxide and 10g/L sodium carbonate in sequence.

7. Use of an exudate activated battery according to claim 1 according to claim 4 for the preparation of an energy supply fabric as an electroactive wound dressing, characterized in that: the reaction temperature in the step 3) is 50-60 ℃.

8. Use of an exudate activated battery as in claim 4 for the preparation of an energy delivery fabric as an electroactive wound dressing, characterized in that: the CYPEAB obtained in the step 4) has a length of 5-7 cm.

9. Use of an exudate activated battery as in claim 4 for the preparation of an energy delivery fabric as an electroactive wound dressing, characterized in that: the number of the CYPEAB connected in series and parallel in the step 5) is 5.