CN117517033A - Perfusion assembly, filtering perfusion device, in-vitro diagnosis analysis instrument and perfusion method - Google Patents

Abstract

The application relates to a perfusion assembly, a filtering perfusion device, an in-vitro diagnostic analysis instrument and a perfusion method. The priming assembly includes a liquid driver, a switch, and a first container. The liquid driver is used for driving the liquid to flow. The switch has a switch inlet in communication with the liquid driver and two switch outlets, one of which is for communication with the priming inlet of the filter. The first container is provided with two liquid inlets, wherein one liquid inlet is communicated with the other switch outlet, and the other liquid inlet is used for being communicated with a filling outlet of the filter. The filling component is communicated with the filter by controlling the liquid driver, the liquid driver fills the reagent into the filter and discharges air therein, and the filling reagent is recovered through the first container, so that the requirements of no loss and no external pollution of the filling reagent are met, the reliability of the testing system is improved, and the condition of inconvenient manual filling operation is avoided.

Description

Perfusion assembly, filtering perfusion device, in-vitro diagnosis analysis instrument and perfusion method

Technical Field

The application relates to the technical field of medical equipment, in particular to a perfusion assembly, a filtering perfusion device, an in-vitro diagnosis analysis instrument and a perfusion method.

Background

In the field of In vitro diagnostics (In-Vitro Diagnostics, IVD), reagents are typically filtered In order to reduce or avoid their impact on the test system. In this case, the general filtering requirement is to filter out particles with a diameter of 0.1um to 10um, and thus the pore size of the filter screen of the selected filter is also 0.1um to 10um. However, inside the filter, air in the reagent can only stay inside the filter due to the surface tension of the reagent; over time (typically half a year), more and more reagent passes through the filter, and more air builds up inside the filter, eventually leading to failure of the reagent to pass through the filter, resulting in priming failure.

To purge the filter of air, manual priming of the filter is required periodically. When the filter is manually filled, the filter is filled with liquid through the needle cylinder until the whole filter is filled with the liquid, and the air in the filter is discharged. The filter needs to be detached separately before pouring, which is inconvenient; in addition, in the manual pouring process, the reagent may be polluted, the reagent required to be used in the subsequent test is affected, and the risk of abnormal test results exists.

Disclosure of Invention

Based on the above, it is necessary to provide a perfusion assembly, a filtering perfusion device, an in-vitro diagnostic and analytical instrument and a perfusion method for solving the problems of inconvenient operation and reagent pollution in the process of manually perfusing a filter.

A priming assembly for priming a filter, the priming assembly comprising:

a liquid driver for driving a liquid to flow;

a switch having a switch inlet in communication with the liquid driver and two switch outlets, one of the switch outlets being for communication with the priming inlet of the filter; and

the first container is provided with two liquid inlets, one liquid inlet is communicated with the other switch outlet, and the other liquid inlet is used for being communicated with a filling outlet of the filter.

In one embodiment, the infusion assembly further comprises: and the second container is provided with a liquid outlet, and the liquid outlet is communicated with the liquid driver.

In one embodiment, the liquid driver is a diaphragm pump.

In one embodiment, the switch is an electromagnetic three-way valve.

In one embodiment, the first container is barrel-shaped.

In one embodiment, the second container is barrel-shaped.

A filtration perfusion device comprising:

a perfusion assembly as described in any one of the embodiments above; and

a filter having a priming inlet in communication with one of the switch outlets of the switch and a priming outlet in communication with the other of the liquid inlets of the first container.

An in vitro diagnostic assay device comprising:

a filtration and perfusion device as described above, the filter further having a filtrate outlet; and

and the controller is electrically connected with the liquid driver and the change-over switch of the filtering and pouring device respectively and is used for controlling the liquid driver and the change-over switch.

A priming method employing a filtration priming device as described above, the priming method comprising:

and the change-over switch is controlled to be communicated with the liquid driver and the filter periodically, and the liquid driver is controlled to drive liquid to flow until the filter is filled with liquid.

In one embodiment, the perfusion method further comprises:

and controlling the change-over switch to be communicated with the liquid driver and the first container according to the actual liquid level in the first container, and controlling the liquid driver to drive the liquid to flow until the actual liquid level in the first container reaches a preset liquid level.

According to the perfusion assembly, the filtering perfusion device, the in-vitro diagnosis analysis instrument and the perfusion method, the switch is arranged, the switch inlet is communicated with the liquid driver, the two switch outlets are respectively communicated with the perfusion inlet of the filter and the first container, and then the communication between the liquid driver and the filter or the communication between the liquid driver and the first container is realized by the switching communication of the switch.

Drawings

FIG. 1 is a block diagram of a perfusion assembly according to one embodiment of the present application.

Fig. 2 is a block diagram of a filter perfusion apparatus according to an embodiment of the present application.

FIG. 3 is a block diagram of an in vitro diagnostic and analysis instrument according to an embodiment of the present application.

Fig. 4 is a flow chart of a perfusion method according to an embodiment of the present application.

Reference numerals illustrate:

100. a perfusion assembly; 110. a liquid driver; 120. a change-over switch; 122. a switch inlet; 124. a switch outlet; 130. a first container; 132. a liquid inlet; 140. a second container; 142. a liquid outlet; 150. a liquid path pipeline;

200. a filtration perfusion device; 210. a filter; 212. a perfusion inlet; 214. a perfusion outlet; 216. a liquid inlet; 218. a filtrate outlet;

300. an in vitro diagnostic analytical instrument; 310. and a controller.

Detailed Description

In order to make the above objects, features and advantages of the present application more comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. This application is, however, susceptible of embodiment in many other forms than those described herein and similar modifications can be made by those skilled in the art without departing from the spirit of the application, and therefore the application is not to be limited to the specific embodiments disclosed below.

In the description of the present application, it should be understood that, if there are terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., these terms refer to the orientation or positional relationship based on the drawings, which are merely for convenience of description and simplification of description, and do not indicate or imply that the apparatus or element referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.

Furthermore, the terms "first," "second," and the like, if any, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present application, the terms "plurality" and "a plurality" if any, mean at least two, such as two, three, etc., unless specifically defined otherwise.

In this application, unless explicitly stated and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly. For example, the two parts can be fixedly connected, detachably connected or integrated; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art as the case may be.

In this application, unless expressly stated or limited otherwise, the meaning of a first feature being "on" or "off" a second feature, and the like, is that the first and second features are either in direct contact or in indirect contact through an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

It will be understood that if an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. If an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein, if any, are for descriptive purposes only and do not represent a unique embodiment.

Referring to fig. 1, fig. 1 shows a block diagram of a perfusion assembly according to an embodiment of the present application, which provides a perfusion assembly 100 for perfusing a filter of an in vitro diagnostic analysis instrument to exhaust air from the filter. The filter has a liquid inlet for inputting the reagent to be filtered and filtering particles in the reagent to reduce or avoid the influence of the reagent on the test system, and a filtrate outlet for outputting the filtered reagent. In order to be able to fill with reagent and to discharge air, the filter is further provided with a filling inlet for filling with reagent and with a filling outlet for discharging air from the filter by filling with reagent. The pouring outlet is used for discharging the poured reagent.

The priming assembly 100 includes a liquid driver 110, a switch 120, and a first container 130. The liquid driver 110 is used to drive the liquid flow. The switch 120 has a switch inlet 122 and two switch outlets 124, the switch inlet 122 being in communication with the liquid driver 110, one of the switch outlets 124 being for communication with the priming inlet of the filter. The first container 130 has two inlets 132, one inlet 132 communicating with the other switch outlet 124, the other inlet 132 being adapted to communicate with the priming outlet of the filter.

Through setting up change over switch 120 to communicate its switch entry 122 with liquid driver 110, two switch export 124 communicate the pouring inlet and the first container 130 of filter respectively, and then through the switch intercommunication of change over switch 120, realize the intercommunication of liquid driver 110 and filter or the intercommunication of liquid driver 110 and first container 130, when needs pouring the filter, control liquid driver 110 and filter intercommunication, liquid driver 110 pours into reagent into the filter, and the air in it is discharged, and retrieve the pouring reagent through the first container 130 that communicates with the pouring outlet of filter, the requirement that pouring reagent is lossless, external pollution does not introduce has been satisfied, thereby promote test system's reliability, avoided the inconvenient condition of manual pouring operation.

In this embodiment, the priming assembly 100 further includes a second container 140, the second container 140 having a liquid outlet 142, the liquid outlet 142 being in communication with the liquid driver 110. The second container 140 is capable of storing a reagent and replenishing the first container 130 with the reagent by the liquid actuator 110 and the switch 120, and it is apparent that the second container 140, the liquid actuator 110 and the first container 130 constitute a liquid replenishing system. When replenishing liquid, the change-over switch 120 is controlled to switch on the liquid driver 110 and the first container 130, and at this time, the liquid driver 110 works to replenish the reagent in the second container 140 into the first container 130; when the filter needs to be filled, the change-over switch 120 is controlled to be switched again to conduct the liquid driver 110 and the filter, at this time, the liquid driver 110 is started, and the reagent in the first container 130 flows into the filling inlet of the filter through the change-over switch 120 under the driving of the liquid driver 110 until the filter is filled with the reagent, and the redundant reagent flows into the first container 130 from the filling outlet of the filter, so that the filling of the filter is realized. It should be noted that, in other embodiments, the priming assembly 100 may not include the second container 140, and the reagent may be directly introduced into the liquid driver 110 through the liquid inlet line.

In this embodiment, the second container 140 is barrel-shaped, and can hold more reagents, so as to meet the requirements of fluid infusion and filter filling of the first container 130. It is understood that in other embodiments, the second container 140 may be a container of other shapes, such as a box, a bottle, a pouch, etc.

In this embodiment, the liquid driver 110 is a diaphragm pump, and the diaphragm pump has no rotating component, and has the same material sucking and protruding modes during operation, the liquid does not flow in a shearing way, the material passing performance is good, and the damage to the material is small, so that the unstable and fragile material can be conveyed. It will be appreciated that in other embodiments, the fluid driver 110 may be a peristaltic pump, a plunger pump, or a syringe, as well as drive the flow of reagents to meet the needs of fluid infusion and perfusion.

In this embodiment, the first container 130 is also barrel-shaped, and has a larger volume, so that more reagent can be replenished. It is understood that in other embodiments, the first container 130 may be a container of other shapes, such as a box, a bottle, a pouch, etc.

It should be noted that, in addition to manual priming, the conventional priming method of the filter may be a diaphragm pump priming, i.e. the diaphragm pump periodically priming to remove air from the filter. The outlet of the diaphragm pump is connected to the priming inlet of the filter, which priming outlet is connected to another container. Starting a diaphragm pump, and injecting liquid into the filter through an injection inlet; after running the membrane pump for a period of time, the filter interior is filled. Compared with manual pouring, the problems of reagent pollution and complex operation can be avoided by pouring the filter through the membrane pump, however, the membrane pump is added for pouring the filter independently and is used once in half a year, and the problems of insufficient utilization rate and cost waste of the membrane pump exist. In addition, a special container is needed for the poured liquid, so that the poured liquid is collected, and reagent waste is caused.

For the conventional diaphragm pump filling mode, in this embodiment, the change-over switch 120 is added on the basis of the diaphragm pump of the existing fluid infusion system, so as to realize the switching between the filling of the filter and the fluid infusion. Specifically, the diaphragm pump of the fluid infusion system is connected to the filter through the change-over switch 120, and the change-over switch 120 and the diaphragm pump of the fluid infusion system are periodically turned on to perfuse the filter; the poured liquid is directly recycled to the first container 130 of the fluid replacement system. Therefore, the filling assembly 100 of the present embodiment utilizes the diaphragm pump and the first container 130 in the existing fluid infusion system, and realizes the filling function of the filter without affecting the original fluid infusion flow, and recovers the filled fluid, thereby avoiding reagent waste and avoiding the need to add a special container.

In this embodiment, the switch 120 is an electromagnetic three-way valve, and the electromagnetic three-way valve has the characteristics of high control precision, high response speed, high reliability, long service life, and the like. It should be noted that, in other embodiments, the switch 120 may be another type of three-way valve, such as a pressure break valve, a screw valve, or a diaphragm valve, and may also implement switching.

In this embodiment, the perfusion module 100 further includes a plurality of liquid channels 150, and the second container 140 is communicated with the liquid driver 110, the liquid driver 110 is communicated with the switch 120, and the switch 120 is communicated with the first container 130 through the liquid channels 150, wherein the other switch outlet 124 of the switch 120 and the other liquid inlet 132 of the first container 130 are also communicated with the filter through the liquid channels 150. Obviously, the specific number of the liquid pipeline 150 can be set according to actual requirements, and different parts of the perfusion assembly 100 can be communicated through one or more than two liquid pipeline 150.

Referring to fig. 2, fig. 2 shows a block diagram of a filtration priming device in an embodiment of the present application, which provides a filtration priming device 200 comprising a priming assembly 100 and a filter 210. The specific structure of the pouring assembly 100 refers to the above embodiment, and since the filtering and pouring device 200 of this embodiment adopts all the technical solutions of all the embodiments, the filtering and pouring device also has all the beneficial effects brought by the technical solutions of the embodiments, which are not described in detail herein. Wherein the filter 210 is provided with a priming inlet 212 and a priming outlet 214, the priming inlet 212 being in communication with one switch outlet 124 of the switch 120, the priming outlet 214 being in communication with the other inlet 132 of the first container 130.

In this embodiment, the filter 210 is further provided with a liquid inlet 216 and a filtrate outlet 218, and the reagent to be filtered enters the filter 210 through the liquid inlet 216 and is filtered, and the filtered filtrate is output from the filtrate outlet 218 and enters the test system through the liquid injection tube set.

Referring to fig. 1, in this embodiment, the filtering and priming device 200 operates as follows:

when the filter 210 needs to be filled, the liquid driver 110 and the filter 210 are controlled to be turned on by the control switch 120, the liquid driver 110 is started, the reagent flows along the first flow direction f1, the liquid driver 110 injects the reagent in the first container 130 into the filter 210 through the filling inlet 212 of the filter 210 until the filter 210 is filled with the reagent, and the redundant reagent or the reagent after being filled flows into the first container 130 from the filling outlet 214 of the filter 210, so that the filling of the filter 210 is completed;

when the fluid replacement system needs fluid replacement, the switch 120 is controlled to switch on the liquid driver 110 and the first container 130, the liquid driver 110 works, the reagent flows along the second flow direction f2, and the reagent in the second container 140 is conveyed into the first container 130 to supplement the reagent in the first container 130, so as to maintain the proper liquid level of the reagent in the first container 130, and avoid insufficient reagent caused by too low liquid level.

Therefore, the filtering and priming device 200 in this embodiment utilizes the diaphragm pump and the first container 130 in the existing fluid infusion system, and implements the priming function of the filter 210 without affecting the original fluid infusion flow; after the device is applied, no loss of the perfusion reagent and no introduction of external pollution are realized, and the reliability of a test system is improved; the problems of insufficient diaphragm pump utilization rate and cost waste caused by adding a diaphragm pump for filling the filter are avoided, and a special container is not required to be added, so that the filled liquid is collected, and reagent waste is avoided.

Referring to fig. 3, fig. 3 is a block diagram illustrating an in-vitro diagnostic and analyzing apparatus according to an embodiment of the present application, and an in-vitro diagnostic and analyzing apparatus 300 provided in an embodiment of the present application includes a filtering and priming device 200 and a controller 310, wherein the controller 310 is electrically connected to the switch 120 and the liquid driver 110 of the filtering and priming device 200, respectively, and is used for controlling the switch 120 and the liquid driver 110. The specific structure of the filtering and filling device 200 refers to the above embodiment, and since the in-vitro diagnostic and analyzing apparatus 300 of this embodiment adopts all the technical solutions of all the above embodiments, the same has all the beneficial effects brought by the technical solutions of the above embodiments, and will not be described in detail herein. The controller 310 controls the switch 120 and the liquid driver 110, so that the automatic filling of the filter can be realized, manual operation is not needed, and the degree of intelligence is improved.

It should be noted that the in vitro diagnostic analysis apparatus 300 includes, but is not limited to, clinical chemistry analysis apparatus, immunochemistry analysis apparatus, blood analysis apparatus, microorganism analysis apparatus, and the like.

Referring to fig. 4, fig. 4 shows a flow chart of a perfusion method according to an embodiment of the present application, and the perfusion method according to an embodiment of the present application adopts the filtering perfusion apparatus 200 or the in-vitro diagnostic and analysis instrument 300 according to the above embodiment, and since the perfusion method according to the embodiment adopts all the technical solutions of all the embodiments, the perfusion method also has all the advantages brought by the technical solutions of the embodiments, and will not be described in detail herein.

In this embodiment, the perfusion method includes:

s100, periodically controlling the switch 120 to communicate the liquid driver 110 with the filter 210, and controlling the liquid driver 110 to drive the liquid to flow until the filter 210 is filled with the liquid, thereby completing the filling.

In step S100, the liquid driver 110 and the filter 210 may be turned on by controlling the switch 120 at intervals of half a year, four months, three months or less, and then the liquid driver 110 is controlled to operate for a period of time, such as 10min, 20min, 30min or more, until the filter 210 is filled with the reagent and the air therein is completely exhausted. The operation time of the liquid driver 110 depends on the volume in the filter 210 and the output flow rate of the liquid driver 110 in unit time; alternatively, a liquid level sensor may be disposed in filter 210, and when the liquid level sensor detects that the liquid in filter 210 is filled, a detection signal is fed back to controller 310, and controller 310 controls liquid driver 110 to stop operating.

In this embodiment, the perfusion method further includes:

s200, according to the actual liquid level in the first container 130, controlling the switch 120 to communicate the liquid driver 110 with the first container 130, and controlling the liquid driver 110 to drive the liquid to flow until the actual liquid level in the first container 130 reaches the preset liquid level.

In step 200, the actual liquid level in the first container 130 may be detected by a liquid level sensor, when the actual liquid level is lower than or equal to the alert liquid level, the liquid level sensor transmits a detected signal to the controller 310, the controller 310 controls the liquid driver 110 to replenish the first container 130 of the liquid replenishing system, and when the liquid level sensor detects that the actual liquid level reaches the preset liquid level, the feedback is sent to the controller 310, and the controller 310 controls the liquid driver 110 to stop working.

In summary, according to the perfusion assembly, the filter perfusion device, the in-vitro diagnostic analysis instrument and the perfusion method, the switch 120 is provided, the switch inlet 122 of the switch is communicated with the liquid driver 110, the two switch outlets 124 are respectively communicated with the perfusion inlet of the filter and the first container 130, and further, the communication between the liquid driver 110 and the filter or the communication between the liquid driver 110 and the first container 130 is realized by the switch communication of the switch 120, when the filter needs to be perfused, the liquid driver 110 is controlled to communicate with the filter, the liquid driver 110 perfuses the reagent into the filter, and the air in the liquid driver is discharged, and the perfused reagent is recovered through the first container 130 communicated with the perfusion outlet of the filter, so that the requirements of no loss and no external pollution of the perfused reagent are met, the reliability of the test system is improved, and the condition of inconvenience in manual perfusion operation is avoided; the membrane pump and the first container 130 in the existing fluid infusion system are utilized, and the filling function of the filter 210 is realized on the premise of not influencing the original fluid infusion flow; after the device is applied, no loss of perfusion reagent and no introduction of external pollution are realized; the problems of insufficient diaphragm pump utilization rate and cost waste caused by adding a diaphragm pump for filling the filter are avoided, and a special container is not required to be added, so that the filled liquid is collected, and reagent waste is avoided.

The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above examples only represent a few embodiments of the present application, which are described in more detail and are not to be construed as limiting the scope of the claims. It should be noted that it would be apparent to those skilled in the art that various modifications and improvements could be made without departing from the spirit of the present application, which would be within the scope of the present application. Accordingly, the scope of protection of the present application is to be determined by the claims appended hereto.

Claims (10)

1. A priming assembly for priming a filter, the priming assembly comprising:

a liquid driver for driving a liquid to flow;

a switch having a switch inlet in communication with the liquid driver and two switch outlets, one of the switch outlets being for communication with the priming inlet of the filter; and

the first container is provided with two liquid inlets, one liquid inlet is communicated with the other switch outlet, and the other liquid inlet is used for being communicated with a filling outlet of the filter.

2. The perfusion assembly of claim 1, further comprising:

and the second container is provided with a liquid outlet, and the liquid outlet is communicated with the liquid driver.

3. The perfusion assembly of claim 1 or 2, wherein the liquid driver is a diaphragm pump.

4. The perfusion assembly of claim 1 or 2, wherein the switch is an electromagnetic three-way valve.

5. The assembly according to claim 1 or 2, wherein the first container is barrel-shaped.

6. The infusion assembly according to claim 2, wherein the second container is barrel-shaped.

7. A filtration perfusion device, comprising:

a perfusion assembly according to any one of claims 1 to 6; and

a filter having a priming inlet in communication with one of the switch outlets of the switch and a priming outlet in communication with the other of the liquid inlets of the first container.

8. An in vitro diagnostic analytical apparatus comprising:

a filtration priming device of claim 7, the filter further having a filtrate outlet; and

and the controller is electrically connected with the liquid driver and the change-over switch of the filtering and pouring device respectively and is used for controlling the liquid driver and the change-over switch.

9. A priming method employing the filtered priming device of claim 7, the priming method comprising:

and the change-over switch is controlled to be communicated with the liquid driver and the filter periodically, and the liquid driver is controlled to drive liquid to flow until the filter is filled with liquid.

10. The perfusion method of claim 9, further comprising:

and controlling the change-over switch to be communicated with the liquid driver and the first container according to the actual liquid level in the first container, and controlling the liquid driver to drive the liquid to flow until the actual liquid level in the first container reaches a preset liquid level.