CN117512127A - SNP locus affecting body length and chest circumference of yaks and application thereof - Google Patents

SNP locus affecting body length and chest circumference of yaks and application thereof Download PDF

Info

Publication number
CN117512127A
CN117512127A CN202311539806.3A CN202311539806A CN117512127A CN 117512127 A CN117512127 A CN 117512127A CN 202311539806 A CN202311539806 A CN 202311539806A CN 117512127 A CN117512127 A CN 117512127A
Authority
CN
China
Prior art keywords
yak
genotype
body length
chest circumference
snp molecular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202311539806.3A
Other languages
Chinese (zh)
Other versions
CN117512127B (en
Inventor
梁春年
张娟香
喇永富
吴晓云
马晓明
阎萍
褚敏
包鹏甲
郭宪
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Original Assignee
Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS filed Critical Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Priority to CN202311539806.3A priority Critical patent/CN117512127B/en
Publication of CN117512127A publication Critical patent/CN117512127A/en
Application granted granted Critical
Publication of CN117512127B publication Critical patent/CN117512127B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/172—Haplotypes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention belongs to the technical field of molecular biotechnology and molecular marker, and particularly relates to SNP loci influencing the body length and chest circumference of yaks and application thereof. The SNP locus is positioned at 55999531 th site of a yak reference genome Bosgu_v3.0 version European Nucleotide Archive accession number GCA_005887515.1, and a mutant base is C or T. The invention also discloses a detection method of the SNP locus and application of the SNP locus in correlation analysis of yak characters, the SNP locus can be applied to auxiliary selective breeding of yak growth characters, and the detection method is quick and accurate; the genotype of the SNP locus is screened to accelerate the breeding progress of the growth character related to the yaks, save the breeding cost and improve the economic benefit of yak breeding.

Description

SNP locus affecting body length and chest circumference of yaks and application thereof
Technical Field
The invention belongs to the technical field of molecular biology detection, and particularly relates to SNP loci influencing the body length and chest circumference of yaks and application thereof.
Background
The yaks are special livestock and poultry living in Qinghai-Tibet plateau and adjacent high mountain areas and sub-high mountain areas, have strong adaptability to high-cold weather, are indispensable resources for maintaining life data and economic income of herdsmen, not only provide beef, milk, fur and the like for people, but also heat the feces as fuel. However, the yak breeding is mainly based on traditional grazing, and the economic development of the yak industry is restricted by the problems of serious grassland degradation, long marketing period, low reproductive capacity of the yaks, and the like, so that the development of the protection, development and utilization of the yak genetic resources, the improvement of the production performance of the yaks and the promotion of the development of the yak industry are of great significance.
Single nucleotide polymorphisms (Single nucleotide polymorphism, SNPs) are variations in DNA sequence that result from mutation of a single nucleotide in the whole genome sequence. Such variations are mainly four types of single gene transitions, transversions, deletions and insertions, but are mostly represented by transitions (transitions) and transversions (transitions). Transitions refer to the substitution of purine bases (A and G) with pyrimidine bases (T and C), and transversions are the substitution of purines with pyrimidines. SNPs occurring in coding regions affect the function of genes resulting in alterations in biological traits and thus can be used as biomarkers associated with certain traits. The method has the characteristics of high density, stable inheritance, automatic analysis and the like, and can be used for marker-assisted breeding, linkage analysis and biodiversity research.
TXK (tyrosine-protein kinase TXK), known as tyrosine protein kinase, is a member of the TEC family, which has a long amino-terminal unique region, comprising a Pleckstrin homology domain and a TEC homology domain, plays an important role in actin cytoskeletal reorganization in response to TCR/CD28, and in addition, helps promote lymphocyte development, activation and viable cell signaling. TXK, also known as RIK, has a genome consisting of 15 exons, which are homologous to exon-intron tissues, with the exception that the amino-terminal region does not include pleckstrin homology domains or other known extended functional regions.
Disclosure of Invention
The invention aims to apply TXK as a candidate gene for molecular marker assisted selection in yak molecular marker assisted breeding so as to shorten the breeding period and improve the growth performance of yaks by breeding. The method specifically comprises the following steps:
in a first aspect, the invention provides an SNP molecular marker related to the growth traits of yak body length and chest circumference, which is characterized in that the SNP molecular marker is positioned at 55999531 th position of a yak reference genome Bosgu_v3.0 version European Nucleotide Archive accession number GCA_005887515.1, and a mutant base is C or T.
In a second aspect, the invention provides an application of a reagent for detecting the SNP molecular marker in the first aspect in detection of yak body length and chest circumference growth traits.
Preferably, according to the SNP molecular markers, the yak genotypes are divided into CC, CT and TT; the 6 month old body length of the genotype CC yak individual is obviously higher than the genotype TT; the 12 month-old chest circumference of the genotype CT yak individual is obviously higher than the genotype TT.
Preferably, the reagents comprise a primer pair for amplifying a nucleotide sequence containing the SNP molecular marker.
Preferably, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1, and the SNP molecular marker is positioned at the 112 th position.
In a third aspect, the invention provides an application of a reagent for detecting the SNP molecular marker in the first aspect in early breeding of yak growth traits.
Preferably, according to the SNP molecular markers, the yak genotypes are divided into CC, CT and TT; the 6 month old body length of the genotype CC yak individual is obviously higher than the genotype TT; the 12 month-old chest circumference of the genotype CT yak individual is obviously higher than the genotype TT.
Preferably, the reagents comprise a primer pair for amplifying a nucleotide sequence containing the SNP molecular marker.
Preferably, the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1, and the SNP molecular marker is positioned at the 112 th position.
In a fourth aspect, the invention provides the use of a specific primer pair for amplifying a nucleotide sequence containing the SNP molecular marker as set forth in the first aspect, for detecting yak body length and chest circumference growth traits; the sequence of the specific primer pair is shown as SEQ ID NO. 2-3.
Preferably, the method for realizing detection of the growth traits of the yaks comprises the following steps:
(1) Extracting yak blood genome DNA as template DNA;
(2) Carrying out PCR amplification on the genome DNA of the yak blood to be detected obtained in the step (1) by utilizing a specific primer pair to obtain a PCR amplification product;
(3) Purifying the PCR amplification product obtained in the step (2) and carrying out genotyping detection, wherein the 6 month old body length of the genotype CC yak individual is obviously higher than the genotype TT; the 12 month-old chest circumference of the genotype CT yak individual is obviously higher than the genotype TT.
In a fifth aspect, the invention provides the use of a specific primer pair for amplifying a nucleotide sequence containing the SNP molecular marker as set forth in the first aspect, for early breeding of yak body length and chest circumference growth traits; the sequence of the specific primer pair is shown as SEQ ID NO. 2-3.
Preferably, the method for realizing the early breeding of the growth traits of the yaks comprises the following steps:
(1) Extracting yak blood genome DNA as template DNA;
(2) Carrying out PCR amplification on the genome DNA of the yak blood to be detected obtained in the step (1) by utilizing a specific primer pair to obtain a PCR amplification product;
(3) Purifying the PCR amplification product obtained in the step (2) and carrying out genotyping detection, wherein the 6 month old body length of the genotype CC yak individual is obviously higher than the genotype TT; the chest circumference of the genotype CT yak individual at 12 months of age is obviously higher than the genotype TT; selecting a yak individual with genotype CC for early breeding of 6-month-old body length character, and selecting a yak individual with genotype CT for early breeding of 12-month-old chest circumference character.
Compared with the prior art, the invention has the following beneficial effects: according to the invention, through analyzing the correlation between the locus genotype and the growth character of the yaks, SNP loci related to the growth character of the yaks body length and chest circumference are found, the SNP molecular marker is positioned at 55999531 th position of a Bosgu_v3.0 version European Nucleotide Archive accession number GCA_005887515.1 of a reference genome of the yaks, and the mutation base is C or T. The length of yak individuals of the genotype CC is significantly higher than that of individuals of the TT type (P < 0.05) at 6 months of age; CT type yak individual is at 12 months of age chest circumference is obviously higher than TT type individual (P < 0.05), and can be used for quick identification of corresponding character through PCR and gene sequencing method, can be used for auxiliary breeding of yak molecular marker, and is not limited by yak variety and age, therefore, in production, CC type and CT type individual can be selected as parents to carry out large-scale breeding, the breeding process of yaks is greatly accelerated, and breeding cost of breeding enterprises can be reduced.
Drawings
FIG. 1 is a graph showing the peak sequencing of three genotypes in the examples of the present invention.
Detailed Description
The invention continuously designs a plurality of pairs of primers on a yak Tyrosine protein Kinase (TXK) gene fragment (with a sequence of SEQ ID NO. 1) to carry out PCR amplification and gene sequencing on yak DNA, wherein one SNP locus is found when a target fragment amplified by one pair of primers (SEQ ID NO.2 and SEQ ID NO. 3) is subjected to genotype analysis, three genotypes are totally found, and one SNP locus is located at 112 th base of the fragment with the sequence of SEQ ID NO.1 through MEGA 7.0 and BioEdit software analysis. Then, the correlation between the genotype of the mutation site of the yak individual and the growth character is analyzed by SPSS19.0 software, and the CC type yak individual is found that the body length of the CC type yak individual is obviously higher than that of the TT type yak individual (P is less than 0.05) at the age of 6 months; the chest circumference of CT type yak individuals at 12 months of age is significantly higher than that of TT type individuals (P < 0.05).
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail with reference to the embodiments. It should be noted that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. The reagents not specifically and individually described in the present invention are all conventional reagents and are commercially available; methods which are not specifically described in detail are all routine experimental methods and are known from the prior art. Various modifications and alterations of this invention may be made by those skilled in the art without departing from the spirit and scope of this invention.
EXAMPLE 1 identification of SNP mutation sites
(1) Sample collection of Ashdan yaks
The invention takes an archen yak variety as a detection object, and collects 232 blood samples of the archen yaks with perfect growth data materials from Datong cattle farm in Qinghai province. The growth phenotypes of the ashdenier yaks were measured at different times, including body weight, height, body length, chest circumference, respectively. EDTA anticoagulant blood collection tube is used for collecting 4ml of blood from jugular vein, and the blood is preserved at-20 ℃.
(2) Isolation, extraction and purification of genomic DNA
UsingBlood Genomic DNAKit kit genomic DNA was extracted from blood samples of Alash denier yaks. The quality and concentration of the DNA was checked using 1.0% agarose gel electrophoresis and an ultraviolet spectrophotometer (Thermo Scientific Nano Drop 2000 c).
(3) Primer design and screening
According to the yak TXK gene published by Ensemble (accession number: ENSBGRG 00000001037), a plurality of pairs of primers are designed on the DNA sequence of the yak TXK gene by using Primer design software Primer 5.0, PCR amplification is carried out on a yak DNA sample, and the result of gene sequencing is analyzed, so that a pair of primers with SNP loci are screened, wherein the Primer sequence information is as follows:
f:5'-GGAAGTGTTGATGTGGTTGGAT-3' (SEQ ID NO. 2);
r:5'-TGAAGAGAGCCAGGGAGAAAC-3' (SEQ ID NO. 3).
(4) PCR amplification of target Gene fragment
The PCR reaction system was 40. Mu.L: 2X Accurate Taq Master Mix (dye plus) 20. Mu.L, 1. Mu.L of DNA template (100 ng/. Mu.L), 1. Mu.L of each of the upstream and downstream primers (10. Mu. Mol/L), and 17. Mu.L of sterile water. PCR amplification procedure: pre-denaturation at 94 ℃ for 30s; denaturation at 98℃for 10s, annealing at 52℃for 30s, elongation at 72℃for 1min,35 cycles; extending at 72 ℃ for 2min, and cooling at 4 ℃. After amplification, the amplified products were detected by electrophoresis on a 1% agarose gel.
(5) Gene sequencing
And (3) sending the PCR reaction liquid after the detection is qualified to Sanger sequencing in two directions of Seamantadine Biotechnology Limited liability company. The amplified target sequence is shown as SEQ ID NO.1, and the SNP locus is positioned at position 112 of the sequence shown as SEQ ID NO. 1. The sequencing peaks at the mutation sites are shown in FIG. 1.
EXAMPLE 2 correlation of different genotypes and growth traits of SNP molecular marker loci
(1) Genotyping
All individuals were repeated in the steps (4) and (5) of example 1, and specific genotypes of the different individuals were determined based on the results of the gene sequencing. Three genotypes were detected in the test population, and the genotype frequencies and allele frequencies are shown in table 1.
The genotyping is carried out on 232 yak blood DNA samples by adopting PCR and gene sequencing, and three genotypes exist at SNP molecular marker loci of yak TXK genes, namely homozygous CC, heterozygous CT and homozygous TT. The three genotypes were found to have frequencies of 0.401 (CC), 0.464 (CT) and 0.134 (TT).
TABLE 1 genotype and allele frequencies of yak SNP loci
(2) SNP genotype and growth trait phenotype value correlation analysis
In order to determine whether the SNP markers prepared by the invention are related to the difference of the growth traits of the yaks, the correlation analysis of the statistical analysis of least squares is carried out on three genotypes of the SNP locus at the 112 position on the fragment of SEQ ID NO.1 and the body weight, height, body length and chest circumference trait phenotype values of the yaks in different periods respectively by using SPSS19.0 software, and the correlation of the genotype of the SNP locus and the growth traits is calculated, and the result is shown in Table 2.
The model used was as follows:
Y j ＝μ+G j +e j the method comprises the steps of carrying out a first treatment on the surface of the Wherein Y is j Representing observed growth trait values; g j A genetic effect representing genotype j; μ representing the total average for each trait; e, e j Representing the effect of the random residual error. Differences between the data sets were tested using LSD multiplex comparison and test results are expressed as mean+ -SE.
TABLE 2 correlation analysis of Gene polymorphisms and growth traits
Note that: the lower case letters of the different superscripts indicate that the difference is significant (P < 0.05), and the x indicates that the difference is significant (P < 0.05)
As can be seen from Table 2, the 55999531bp locus polymorphism of the chromosome 6 of the yaks has obvious correlation (P < 0.05) with the 6 month-old body length and the 12 month-old chest circumference of the yaks, and the body length of the yaks of the individuals with the genotype CC homozygote at the age of 6 months is obviously higher than that of the individuals with the genotype TT; CT-type yak individuals had significantly higher chest circumference at 12 months of age than TT genotype individuals (P < 0.05).
This example identifies a SNP marker that is significantly associated with the growth traits of yaks in length and chest circumference, so selection of dominant genotype individuals can be selected and will help to improve the growth traits of yaks.
By combining the results, the mutation site can be used as a potential genetic marker for improving the production performance of the yaks in the auxiliary selection of the high-yield yaks.
The foregoing is merely illustrative of specific embodiments of the present invention, and the scope of the invention is not limited thereto, but any modifications, equivalents, improvements and alternatives falling within the spirit and principles of the present invention will be apparent to those skilled in the art within the scope of the present invention.

Claims (10)

1. A SNP molecular marker affecting the length and chest circumference of a yak, wherein said SNP molecular marker is located at position 55999531 of the yak reference genome bosgu_v3.0 version European Nucleotide Archive accession gca_005887515.1, and the mutated base is C or T.
2. Use of a reagent for detecting the SNP molecular markers of claim 1 for detecting yak body length and chest circumference growth traits.
3. The use of a reagent for detecting the SNP molecular markers as set forth in claim 1 in the early breeding of yak body length and chest circumference growth traits.
4. The use according to claim 2 or 3, wherein the yak genotypes are classified into CC, CT and TT according to the SNP molecular markers; the 6 month old body length of the genotype CC yak individual is obviously higher than the genotype TT; the 12 month-old chest circumference of the genotype CT yak individual is obviously higher than the genotype TT.
5. The use according to claim 4, wherein the reagent comprises a primer pair for amplifying a nucleotide sequence containing the SNP molecular marker.
6. The use according to claim 5, wherein the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1, and the SNP molecular marker is located at position 112.
7. Use of a specific primer pair for amplifying a nucleotide sequence containing the SNP molecular marker of claim 1 for detecting yak body length and chest circumference growth traits; the sequence of the specific primer pair is shown as SEQ ID NO. 2-3.
8. The use of claim 7 wherein the method for effecting the detection of yak body length and chest circumference growth traits comprises:
(1) Extracting yak blood genome DNA as template DNA;
(2) Carrying out PCR amplification on the genome DNA of the yak blood to be detected obtained in the step (1) by utilizing a specific primer pair to obtain a PCR amplification product;
(3) Purifying the PCR amplification product obtained in the step (2) and carrying out genotyping detection, wherein the 6 month old body length of the genotype CC yak individual is obviously higher than the genotype TT; the 12 month-old chest circumference of the genotype CT yak individual is obviously higher than the genotype TT.
9. Use of a specific primer pair for amplifying a nucleotide sequence containing the SNP molecular marker of claim 1 for early breeding of yak body length and chest circumference growth traits; the sequence of the specific primer pair is shown as SEQ ID NO. 2-3.
10. The use of claim 9 wherein the method for effecting early breeding of yak body length and chest circumference growth traits comprises:
(1) Extracting yak blood genome DNA as template DNA;
(2) Carrying out PCR amplification on the genome DNA of the yak blood to be detected obtained in the step (1) by utilizing a specific primer pair to obtain a PCR amplification product;
(3) Purifying the PCR amplification product obtained in the step (2) and carrying out genotyping detection, wherein the 6 month old body length of the genotype CC yak individual is obviously higher than the genotype TT; the chest circumference of the genotype CT yak individual at 12 months of age is obviously higher than the genotype TT; selecting a yak individual with genotype CC for early breeding of 6-month-old body length character, and selecting a yak individual with genotype CT for early breeding of 12-month-old chest circumference character.
CN202311539806.3A 2023-11-18 2023-11-18 SNP locus affecting body length and chest circumference of yaks and application thereof Active CN117512127B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311539806.3A CN117512127B (en) 2023-11-18 2023-11-18 SNP locus affecting body length and chest circumference of yaks and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311539806.3A CN117512127B (en) 2023-11-18 2023-11-18 SNP locus affecting body length and chest circumference of yaks and application thereof

Publications (2)

Publication Number Publication Date
CN117512127A true CN117512127A (en) 2024-02-06
CN117512127B CN117512127B (en) 2024-06-21

Family

ID=89747313

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202311539806.3A Active CN117512127B (en) 2023-11-18 2023-11-18 SNP locus affecting body length and chest circumference of yaks and application thereof

Country Status (1)

Country Link
CN (1) CN117512127B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104046621A (en) * 2013-03-14 2014-09-17 中国农业大学 Porcine anti-porcine reproductive and respiratory syndrome related SNP label in tyrosine protein kinase JAK2 gene and application thereof
CN105441567A (en) * 2016-01-05 2016-03-30 甘肃农业大学 Detection method of yak FOXO1 gene single nucleotide polymorphism and kit thereof
CN105441569A (en) * 2016-01-05 2016-03-30 甘肃农业大学 Detection method of yak FOXO3 gene single nucleotide polymorphism and kit thereof
CN108251540A (en) * 2018-02-07 2018-07-06 西藏自治区农牧科学院畜牧兽医研究所 The application of yak full-length genome SNP site and detection primer sets and kit

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104046621A (en) * 2013-03-14 2014-09-17 中国农业大学 Porcine anti-porcine reproductive and respiratory syndrome related SNP label in tyrosine protein kinase JAK2 gene and application thereof
CN105441567A (en) * 2016-01-05 2016-03-30 甘肃农业大学 Detection method of yak FOXO1 gene single nucleotide polymorphism and kit thereof
CN105441569A (en) * 2016-01-05 2016-03-30 甘肃农业大学 Detection method of yak FOXO3 gene single nucleotide polymorphism and kit thereof
CN108251540A (en) * 2018-02-07 2018-07-06 西藏自治区农牧科学院畜牧兽医研究所 The application of yak full-length genome SNP site and detection primer sets and kit
WO2019153294A1 (en) * 2018-02-07 2019-08-15 西藏自治区农牧科学院畜牧兽医研究所 Application for yak whole genome snp locus and primer group and kit used for detection

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANEET KOUR等: "Genomic Diversity Profiling and Breed-Specific Evolutionary Signatures of Selection in Arunachali Yak", 《GENES》, 28 January 2022 (2022-01-28), pages 1 - 16 *
李天科等: "甘南牦牛GDF-10基因多态与生产性状的相关性分析", 《中国农业科学》, vol. 47, no. 01, 31 January 2014 (2014-01-31), pages 161 - 169 *
梁春年等: "牦牛IGF-IR基因克隆及其序列分析", 《华北农学报》, vol. 33, no. 4, 31 August 2018 (2018-08-31), pages 39 - 45 *
马士龙等: "基于GBS简化基因组测序评估3个麦洼牦牛保种群的遗传结构研究", 《草业学报》, vol. 31, no. 9, 30 September 2022 (2022-09-30), pages 183 - 194 *

Also Published As

Publication number Publication date
CN117512127B (en) 2024-06-21

Similar Documents

Publication Publication Date Title
CN111705140B (en) SNPs molecular marker related to weight traits and application thereof in Hu sheep assisted breeding
CN116837112B (en) SNP molecular marker related to yak growth traits and application thereof
KR101929391B1 (en) Novel SNP marker for discriminating increasedthe number of nipples of pigs and use thereof
KR20180077873A (en) SNP markers for selection of marker-assisted backcross in watermelon
CN117051127B (en) SNP locus related to yak growth traits and application thereof
US20170283854A1 (en) Multiplexed pcr assay for high throughput genotyping
US20060166244A1 (en) DNA markers for increased milk production in cattle
CN111334588B (en) Molecular marker related to black and brown feather characters of duck and application thereof
CN116377082B (en) Application of sheep LCORL gene single nucleotide polymorphism marker in growth trait selection
CN117512127B (en) SNP locus affecting body length and chest circumference of yaks and application thereof
KR101825497B1 (en) Kits for Detecting Equine Maternal lineage and Predicting Athletic Ability with Single Nucleotide Polymorphism and Method for Detection of Equine Maternal lineage and Prediction of Athletic Ability thereby
CN117265133B (en) SNP locus related to fat and lactose content of yak milk and application
CN117286260B (en) SNP locus related to yak dairy quality traits and application thereof
KR101985659B1 (en) Method for identification of Baekwoo breed using single nucleotide polymorphism markers
CN117187414B (en) SNP locus related to yak milk casein and protein content and application thereof
CN114107520B (en) Pig intramuscular fat SNP molecular marker and application thereof
CN115873962B (en) Method for identifying Tiger macula cloud Ling cattle variety by utilizing SETX gene mononucleotide genetic marker
CN116162714B (en) Haplotype molecular marker related to intramuscular fat traits of pigs in SYK gene and application
CN118086517B (en) SNP molecular marker combination for detecting propagation traits of Mola buffalo and application
CN116083604B (en) SNP molecular marker affecting sheep weaning weight and application thereof
CN108277286B (en) Pig IGF1R gene molecular marker and special primer thereof
KR102083675B1 (en) Method for identification of Chikso breed using single nucleotide polymorphism markers
CN117867133A (en) Application of PDGFD gene upstream SNP marker in sheep variety tail type selection
CN118166125A (en) SNP locus related to yak milk density and milk fat content and application thereof
CN117867132A (en) Application of downstream SNP marker of BMP2 gene in tail type selection of sheep variety

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant