CN117510675B - Method for extracting strawberry polysaccharide serving as strawberry active ingredient - Google Patents
Method for extracting strawberry polysaccharide serving as strawberry active ingredient Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses an extraction method of strawberry polysaccharide serving as an active ingredient of strawberries, which comprises the following steps: s1, mixing cleaned strawberries with water, crushing, and performing ultrasonic extraction to obtain a strawberry extract; s2, centrifuging the strawberry extract, collecting supernatant, and filtering to obtain strawberry filtrate; s3, adding rice bran fatty alkanol and carnauba wax into the strawberry filtrate, stirring, standing, filtering, and concentrating the filtrate to obtain a strawberry extract; the extraction method of the invention does not need to adopt high temperature and does not need to add substances such as enzyme, thus greatly retaining the original flavor and nutrition components of the strawberries, and simultaneously adding rice bran fatty alkanol and carnauba wax into the extraction solution, thereby improving the content of strawberry polysaccharide in the strawberry extract.
Description
Technical Field
The invention belongs to the field of extraction methods, and particularly relates to an extraction method of strawberry polysaccharide serving as an active ingredient of strawberries.
Background
The strawberry is perennial herb of Fragaria of Rosaceae, has bright color and unique fruit flavor. It is rich in bioactive substances such as vitamin C, polyphenol and flavone, and the content of the bioactive substances is closely related to the antioxidant capacity. The strawberry extract not only has rich nutrition and commercial value, but also has higher medicinal and medical care values.
The strawberry polysaccharide is acidic heteropolysaccharide, can obviously improve the expression level of Bcl-2 protein produced by lipopolysaccharide-induced mouse macrophages, and plays a good anti-inflammatory role by up-regulating anti-inflammatory factors (IL-10) and down-regulating pro-inflammatory factors (interleukins (IL) -1b and IL-6), and has an inhibitory effect on alpha-glucosidase and alpha-amylase.
Most of the existing methods for extracting the strawberries need to be added with chemical substances, enzymes or heated at high temperature to extract the strawberries, so that the original flavor and nutrition components of the strawberries are damaged to a certain extent, and high-content strawberry polysaccharide is difficult to obtain. Such as: CN107568520a discloses a preparation method of strawberry juice, which comprises the following steps: firstly, removing the fruit stalks and the fruit pedicles of the strawberries, and cleaning; color protection; adding drinking water and crushing; adding pectase and cellulase for enzymolysis after ultrasonic treatment; squeezing, filtering, degassing and homogenizing the strawberry pulp after enzymolysis to obtain strawberry juice; adding granulated sugar, citric acid, apple pectin, vitamin C and lactobacillus into strawberry juice; homogenizing the prepared strawberry juice, sterilizing by ultrahigh pressure homogenization, and then aseptically filling to obtain the strawberry juice product. The strawberry juice prepared by the process and the formula has the advantages of high juice yield, less nutrient loss, prominent strawberry fragrance, bright red color, mellow taste, proper sweet and sour taste, no suspension and layering phenomenon, no preservative addition and long storage time.
Therefore, the extraction method capable of extracting strawberry polysaccharide from strawberry at low temperature without adding substances such as enzyme and the like to destroy original flavor and nutrition of strawberry has certain market value.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the strawberry polysaccharide extraction method for strawberry active ingredients, which does not need to adopt high temperature and does not need to add substances such as enzymes, so that the original flavor and nutrition components of strawberries are greatly reserved, and meanwhile, rice bran fatty alkanol and carnauba wax are added into the extraction solution, so that the strawberry polysaccharide content in the strawberry extract is improved.
The invention aims to provide an extraction method of strawberry polysaccharide serving as an active ingredient of strawberries, which comprises the following steps:
s1, mixing cleaned strawberries with water, crushing, and performing ultrasonic extraction to obtain a strawberry extract;
s2, centrifuging the strawberry extract, collecting supernatant, and filtering to obtain strawberry filtrate;
s3, adding rice bran fatty alkanol and carnauba wax into the strawberry filtrate, stirring, standing, filtering, and concentrating the filtrate to obtain the strawberry extract.
Preferably, in S1, the power of the ultrasonic extraction is 20 to 50kw.
More preferably, in S1, the power of the ultrasonic extraction is 35KW.
Preferably, in S1, the temperature of the ultrasonic extraction is 10-30 ℃.
More preferably, in S1, the temperature of the ultrasonic extraction is 20 ℃.
Preferably, in S1, the ultrasonic extraction time is 0.5 to 1 hour.
More preferably, in S1, the time of the ultrasonic extraction is 0.8 hours.
Preferably, in S1, the mass ratio of the strawberry to the water is 1: 5-10.
More preferably, in S1, the mass ratio of strawberry to water is 1:8.
preferably, in the step S2, the centrifugation includes a primary centrifugation and a secondary centrifugation, the rotational speed of the primary centrifugation is 3800-6000 r/min, the supernatant fluid of the primary centrifugation is collected for the secondary centrifugation, and the rotational speed of the secondary centrifugation is 14000-16000 r/min.
More preferably, in S2, the centrifugation includes a primary centrifugation at a rotational speed of 5000r/min and a secondary centrifugation at a rotational speed of 15000r/min by collecting supernatant of the primary centrifugation and performing the secondary centrifugation.
Preferably, in S3, the mass ratio of the strawberry filtrate to the rice bran fatty alkanol to the carnauba wax is 100: 0.5-1: 0.3 to 0.8.
Preferably, in S3, the standing time is 1 to 3 hours.
More preferably, in S3, the time of the standing is 2 hours.
Preferably, in S3, adding activated carbon to the strawberry filtrate is further included.
Preferably, the mass ratio of the strawberry filtrate to the activated carbon is 100: 2-5.
Preferably, in S3, the concentration is reduced pressure concentration at 60-70 ℃.
More preferably, in S3, the concentration is reduced pressure concentration at 65 ℃.
Compared with the prior art, the invention has the following beneficial effects:
(1) The extraction method of the invention can extract the strawberries at low temperature, which is different from the traditional extraction process that the strawberries are extracted at high temperature or substances such as enzyme are added, and the original flavor and nutrition components of the strawberries are greatly reserved.
(2) According to the invention, a small amount of rice bran fatty alkanol and carnauba wax are added into the extracting solution, the rice bran fatty alkanol contains polar hydroxyl groups and has hydrogen bond action with polar groups of strawberry polysaccharide, so that the strawberry polysaccharide is enriched in the extracting process, and the carnauba wax wraps other substances in the strawberry extract, and under the combined action of the rice bran fatty alkanol and the carnauba wax, the extracting rate of the strawberry polysaccharide is greatly improved; since rice bran fatty alkanol and carnauba wax are themselves conventional pharmaceutical excipients and are poorly soluble in water, they can be removed by simple filtration.
Detailed Description
In order to make the technical solution of the present invention better understood by those skilled in the art, the technical solution of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
Example 1: and (5) extracting strawberries.
S1, cutting 5kg of cleaned strawberries into small pieces of 0.1cm-0.2cm by using a traditional Chinese medicine slicing machine, adding 40kg of water, crushing the strawberry pieces by using a high-efficiency turbine crusher, adding the crushed strawberry liquid into an ultrasonic device for ultrasonic extraction, wherein the ultrasonic extraction power is 35KW, the ultrasonic extraction temperature is 20 ℃, and the ultrasonic extraction time is 0.8 hour, so as to obtain strawberry extract;
s2, conveying the strawberry extracting solution to a buffer tank of a tripodia centrifugal machine by a pump, starting centrifugation, wherein the centrifugation comprises primary centrifugation and secondary centrifugation, the rotation speed of the primary centrifugation is 5000r/min, collecting supernatant fluid of the primary centrifugation for secondary centrifugation, the rotation speed of the secondary centrifugation is 15000r/min, collecting supernatant fluid of the secondary centrifugation, and filtering the supernatant fluid of the secondary centrifugation to obtain strawberry filtrate;
s3, adding 0.05kg of bran fatty alkanol, 0.08kg of carnauba wax and 0.3kg of active carbon into 10kg of strawberry filtrate prepared in the step S2, uniformly stirring, standing for 2 hours, filtering, and concentrating the filtrate under reduced pressure at 65 ℃ to obtain the strawberry extract.
Example 2: and (5) extracting strawberries.
S1, cutting 5kg of cleaned strawberries into small pieces of 0.1cm-0.2cm by using a traditional Chinese medicine slicing machine, adding 40kg of water, crushing the strawberry pieces by using a high-efficiency turbine crusher, adding the crushed strawberry liquid into an ultrasonic device for ultrasonic extraction, wherein the ultrasonic extraction power is 35KW, the ultrasonic extraction temperature is 20 ℃, and the ultrasonic extraction time is 0.8 hour, so as to obtain strawberry extract;
s2, conveying the strawberry extracting solution to a buffer tank of a tripodia centrifugal machine by a pump, starting centrifugation, wherein the centrifugation comprises primary centrifugation and secondary centrifugation, the rotation speed of the primary centrifugation is 5000r/min, collecting supernatant fluid of the primary centrifugation for secondary centrifugation, the rotation speed of the secondary centrifugation is 15000r/min, collecting supernatant fluid of the secondary centrifugation, and filtering the supernatant fluid of the secondary centrifugation to obtain strawberry filtrate;
s3, adding 0.1kg of bran fatty alkanol, 0.03kg of carnauba wax and 0.3kg of active carbon into 10kg of strawberry filtrate prepared in the step S2, uniformly stirring, standing for 2 hours, filtering, and concentrating the filtrate under reduced pressure at 65 ℃ to obtain the strawberry extract.
Example 3: and (5) extracting strawberries.
S1, cutting 5kg of cleaned strawberries into small pieces of 0.1cm-0.2cm by using a traditional Chinese medicine slicing machine, adding 40kg of water, crushing the strawberry pieces by using a high-efficiency turbine crusher, adding the crushed strawberry liquid into an ultrasonic device for ultrasonic extraction, wherein the ultrasonic extraction power is 35KW, the ultrasonic extraction temperature is 20 ℃, and the ultrasonic extraction time is 0.8 hour, so as to obtain strawberry extract;
s2, conveying the strawberry extracting solution to a buffer tank of a tripodia centrifugal machine by a pump, starting centrifugation, wherein the centrifugation comprises primary centrifugation and secondary centrifugation, the rotation speed of the primary centrifugation is 5000r/min, collecting supernatant fluid of the primary centrifugation for secondary centrifugation, the rotation speed of the secondary centrifugation is 15000r/min, collecting supernatant fluid of the secondary centrifugation, and filtering the supernatant fluid of the secondary centrifugation to obtain strawberry filtrate;
s3, adding 0.08kg of bran fatty alkanol, 0.05kg of carnauba wax and 0.3kg of active carbon into 10kg of strawberry filtrate prepared in the step S2, uniformly stirring, standing for 2 hours, filtering, and concentrating the filtrate under reduced pressure at 65 ℃ to obtain the strawberry extract.
Comparative example 1: and (5) extracting strawberries.
S1, cutting 5kg of cleaned strawberries into small pieces of 0.1cm-0.2cm by using a traditional Chinese medicine slicing machine, adding 40kg of water, crushing the strawberry pieces by using a high-efficiency turbine crusher, adding the crushed strawberry liquid into an ultrasonic device for ultrasonic extraction, wherein the ultrasonic extraction power is 35KW, the ultrasonic extraction temperature is 20 ℃, and the ultrasonic extraction time is 0.8 hour, so as to obtain strawberry extract;
s2, conveying the strawberry extracting solution to a buffer tank of a tripodia centrifugal machine by a pump, starting centrifugation, wherein the centrifugation comprises primary centrifugation and secondary centrifugation, the rotation speed of the primary centrifugation is 5000r/min, collecting supernatant fluid of the primary centrifugation for secondary centrifugation, the rotation speed of the secondary centrifugation is 15000r/min, collecting supernatant fluid of the secondary centrifugation, and filtering the supernatant fluid of the secondary centrifugation to obtain strawberry filtrate;
s3, adding 0.08kg of polyethylene glycol with molecular weight of 600, 0.05kg of carnauba wax and 0.3kg of active carbon into 10kg of strawberry filtrate prepared in the step S2, uniformly stirring, standing for 2 hours, filtering, and concentrating the filtrate under reduced pressure at 65 ℃ to obtain the strawberry extract.
Comparative example 2: and (5) extracting strawberries.
S1, cutting 5kg of cleaned strawberries into small pieces of 0.1cm-0.2cm by using a traditional Chinese medicine slicing machine, adding 40kg of water, crushing the strawberry pieces by using a high-efficiency turbine crusher, adding the crushed strawberry liquid into an ultrasonic device for ultrasonic extraction, wherein the ultrasonic extraction power is 35KW, the ultrasonic extraction temperature is 20 ℃, and the ultrasonic extraction time is 0.8 hour, so as to obtain strawberry extract;
s2, conveying the strawberry extracting solution to a buffer tank of a tripodia centrifugal machine by a pump, starting centrifugation, wherein the centrifugation comprises primary centrifugation and secondary centrifugation, the rotation speed of the primary centrifugation is 5000r/min, collecting supernatant fluid of the primary centrifugation for secondary centrifugation, the rotation speed of the secondary centrifugation is 15000r/min, collecting supernatant fluid of the secondary centrifugation, and filtering the supernatant fluid of the secondary centrifugation to obtain strawberry filtrate;
s3, adding 0.08kg of bran fatty alkanol, 0.05kg of 2-hydroxypropyl-beta-cyclodextrin and 0.3kg of active carbon into 10kg of strawberry filtrate prepared in the step S2, uniformly stirring, standing for 2 hours, filtering, and concentrating the filtrate under reduced pressure at 65 ℃ to obtain the strawberry extract.
Comparative example 3: and (5) extracting strawberries.
S1, cutting 5kg of cleaned strawberries into small pieces of 0.1cm-0.2cm by using a traditional Chinese medicine slicing machine, adding 40kg of water, crushing the strawberry pieces by using a high-efficiency turbine crusher, adding the crushed strawberry liquid into an ultrasonic device for ultrasonic extraction, wherein the ultrasonic extraction power is 35KW, the ultrasonic extraction temperature is 20 ℃, and the ultrasonic extraction time is 0.8 hour, so as to obtain strawberry extract;
s2, conveying the strawberry extracting solution to a buffer tank of a tripodia centrifugal machine by a pump, starting centrifugation, wherein the centrifugation comprises primary centrifugation and secondary centrifugation, the rotation speed of the primary centrifugation is 5000r/min, collecting supernatant fluid of the primary centrifugation for secondary centrifugation, the rotation speed of the secondary centrifugation is 15000r/min, collecting supernatant fluid of the secondary centrifugation, and filtering the supernatant fluid of the secondary centrifugation to obtain strawberry filtrate;
s3, adding 0.13kg of bran fatty alkanol and 0.3kg of active carbon into 10kg of strawberry filtrate prepared in the step S2, uniformly stirring, standing for 2 hours, filtering, and concentrating the filtrate under reduced pressure at 65 ℃ to obtain the strawberry extract.
Comparative example 4: and (5) extracting strawberries.
S1, cutting 5kg of cleaned strawberries into small pieces of 0.1cm-0.2cm by using a traditional Chinese medicine slicing machine, adding 40kg of water, crushing the strawberry pieces by using a high-efficiency turbine crusher, adding the crushed strawberry liquid into an ultrasonic device for ultrasonic extraction, wherein the ultrasonic extraction power is 35KW, the ultrasonic extraction temperature is 20 ℃, and the ultrasonic extraction time is 0.8 hour, so as to obtain strawberry extract;
s2, conveying the strawberry extracting solution to a buffer tank of a tripodia centrifugal machine by a pump, starting centrifugation, wherein the centrifugation comprises primary centrifugation and secondary centrifugation, the rotation speed of the primary centrifugation is 5000r/min, collecting supernatant fluid of the primary centrifugation for secondary centrifugation, the rotation speed of the secondary centrifugation is 15000r/min, collecting supernatant fluid of the secondary centrifugation, and filtering the supernatant fluid of the secondary centrifugation to obtain strawberry filtrate;
s3, adding 0.13kg of carnauba wax and 0.3kg of activated carbon into 10kg of strawberry filtrate prepared in the step S2, uniformly stirring, standing for 2 hours, filtering, and concentrating the filtrate under reduced pressure at 65 ℃ to obtain a strawberry extract.
Comparative example 5: and (5) extracting strawberries.
S1, cutting 5kg of cleaned strawberries into small pieces of 0.1cm-0.2cm by using a traditional Chinese medicine slicing machine, adding 40kg of water, crushing the strawberry pieces by using a high-efficiency turbine crusher, adding the crushed strawberry liquid into an ultrasonic device for ultrasonic extraction, wherein the ultrasonic extraction power is 35KW, the ultrasonic extraction temperature is 20 ℃, and the ultrasonic extraction time is 0.8 hour, so as to obtain strawberry extract;
s2, conveying the strawberry extracting solution to a buffer tank of a tripodia centrifugal machine by a pump, starting centrifugation, wherein the centrifugation comprises primary centrifugation and secondary centrifugation, the rotation speed of the primary centrifugation is 5000r/min, collecting supernatant fluid of the primary centrifugation for secondary centrifugation, the rotation speed of the secondary centrifugation is 15000r/min, collecting supernatant fluid of the secondary centrifugation, and filtering the supernatant fluid of the secondary centrifugation to obtain strawberry filtrate;
s3, adding 0.3kg of active carbon into 10kg of the strawberry filtrate prepared in the step S2, uniformly stirring, standing for 2 hours, filtering, and concentrating the filtrate under reduced pressure at 65 ℃ to obtain the strawberry extract.
The total strawberry polysaccharide content of the strawberry extracts of examples 1 to 3 and comparative examples 1 to 5 was measured by a phenol-sulfuric acid method, and the results are shown in Table 1, with D-glucose as a standard.
Table 1. Results of the test of the total strawberry polysaccharide content in the strawberry extracts of examples 1 to 3 and comparative examples 1 to 5.
As can be seen from Table 1, the extraction methods of examples 1-3 of the present application can greatly increase the total strawberry polysaccharide content in the strawberry extract.
Finally, it should be noted that the above-mentioned embodiments are merely for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made to the specific embodiments of the present invention after reading the present specification, and these modifications and variations do not depart from the scope of the invention as claimed in the pending claims.
Claims (10)
1. The extraction method of strawberry polysaccharide as strawberry active ingredient is characterized by comprising the following steps:
s1, mixing cleaned strawberries with water, crushing, and performing ultrasonic extraction to obtain a strawberry extract;
s2, centrifuging the strawberry extract, collecting supernatant, and filtering to obtain strawberry filtrate;
s3, adding rice bran fatty alkanol and carnauba wax into the strawberry filtrate, stirring, standing, filtering, and concentrating the filtrate to obtain the strawberry extract.
2. The method for extracting strawberry polysaccharide as claimed in claim 1, wherein in S1, the power of ultrasonic extraction is 20-50 kw.
3. The method for extracting strawberry polysaccharide as claimed in claim 1, wherein in S1, the temperature of ultrasonic extraction is 10-30 ℃.
4. The method for extracting strawberry polysaccharide as claimed in claim 1, wherein in S1, the ultrasonic extraction time is 0.5-1 hour.
5. The method for extracting strawberry polysaccharide as claimed in claim 1, wherein in S1, the mass ratio of strawberry to water is 1: 5-10.
6. The method for extracting strawberry polysaccharide as claimed in claim 1, wherein in S2, the centrifugation includes a primary centrifugation and a secondary centrifugation, the rotational speed of the primary centrifugation is 3800-6000 r/min, the supernatant of the primary centrifugation is collected for the secondary centrifugation, and the rotational speed of the secondary centrifugation is 14000-16000 r/min.
7. The method for extracting strawberry polysaccharide as claimed in claim 1, wherein in S3, the mass ratio of the strawberry filtrate to rice bran fatty alkanol to carnauba wax is 100: 0.5-1: 0.3 to 0.8.
8. The method for extracting strawberry polysaccharide as claimed in claim 1, wherein in S3, the standing time is 1 to 3 hours.
9. The method for extracting strawberry polysaccharide as claimed in claim 1, wherein in S3, activated carbon is added to the strawberry filtrate.
10. The method for extracting strawberry polysaccharide as claimed in claim 1, wherein in S3, the concentration is reduced pressure concentration at 60-70 ℃.
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| CN113101232A (en) * | 2021-04-13 | 2021-07-13 | 美尚(广州)化妆品股份有限公司 | Toner-coated eyebrow pencil and preparation method thereof |
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| CN110963863A (en) * | 2019-12-25 | 2020-04-07 | 四川金雅科技有限公司 | Composite oil phase for powder emulsion explosive and preparation method thereof |
| CN111956626A (en) * | 2020-08-19 | 2020-11-20 | 浙江新维士生物科技有限公司 | Production process of anti-adhesion soft capsule and anti-adhesion combined liquid thereof |
| CN113101232A (en) * | 2021-04-13 | 2021-07-13 | 美尚(广州)化妆品股份有限公司 | Toner-coated eyebrow pencil and preparation method thereof |
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