CN117487027B - Multivalent nanometer chelating peptide and application thereof - Google Patents
Multivalent nanometer chelating peptide and application thereof Download PDFInfo
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- CN117487027B CN117487027B CN202311440621.7A CN202311440621A CN117487027B CN 117487027 B CN117487027 B CN 117487027B CN 202311440621 A CN202311440621 A CN 202311440621A CN 117487027 B CN117487027 B CN 117487027B
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 39
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 37
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- 108010075254 C-Peptide Proteins 0.000 claims abstract description 8
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- 239000012581 transferrin Substances 0.000 claims abstract description 7
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 5
- 238000001179 sorption measurement Methods 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 9
- 241000588724 Escherichia coli Species 0.000 claims description 8
- 239000012620 biological material Substances 0.000 claims description 7
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- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
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- 102000037865 fusion proteins Human genes 0.000 description 1
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- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
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- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 1
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- 239000008101 lactose Substances 0.000 description 1
- HWSZZLVAJGOAAY-UHFFFAOYSA-L lead(II) chloride Chemical compound Cl[Pb]Cl HWSZZLVAJGOAAY-UHFFFAOYSA-L 0.000 description 1
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- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
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- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
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- 150000007523 nucleic acids Chemical group 0.000 description 1
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- 239000013049 sediment Substances 0.000 description 1
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- 229910052709 silver Inorganic materials 0.000 description 1
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- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/285—Treatment of water, waste water, or sewage by sorption using synthetic organic sorbents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Environmental & Geological Engineering (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
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- Hydrology & Water Resources (AREA)
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- Peptides Or Proteins (AREA)
Abstract
The invention relates to the technical field of heavy metal pollution repair, in particular to a multivalent nano chelating peptide and application thereof. A multivalent nano-chelating peptide, comprising transferrin, a connecting peptide and an artificial plant chelating peptide which are sequentially connected; the amino acid sequence of the transferrin is shown as SEQ ID NO:1 is shown in the specification; the amino acid sequence of the artificial plant chelating peptide is shown as SEQ ID NO: 2. After the multivalent nano chelating peptide provided by the invention is expressed by engineering bacteria, the multivalent nano chelating peptide has higher capability of absorbing heavy metal ions and good selectivity specificity, has good application value, and provides a new thought and a new technology for bioremediation of heavy metal polluted environments.
Description
Technical Field
The invention relates to the technical field of heavy metal pollution repair, in particular to a multivalent nano chelating peptide and application thereof.
Background
Heavy metals, which are metals with a density of greater than 4.5g/cm 3, including gold, silver, copper, iron, mercury, lead, cadmium, etc., accumulate in the human body to a certain extent, and cause chronic poisoning.
Heavy metals are difficult to biodegrade, but can be enriched thousands of times under the biological amplification of a food chain, and finally enter the human body. Heavy metals can interact strongly with proteins and enzymes in the human body, so that they lose activity and can accumulate in certain organs of the human body, causing chronic poisoning.
Heavy metal wastewater is generated in a large amount in the process of urban treatment and industrialization, and ecological environment and human health are seriously threatened, so that the method has important significance for research on removal of heavy metals in water environment. In the prior art, heavy metals are mainly removed by methods such as chemical precipitation, electrolysis, ion exchange, reverse osmosis, active carbon adsorption and the like, and compared with the methods, the biological adsorption has the characteristics of environment friendliness, easiness in obtaining, environment friendliness and the like, and is paid attention to the field of heavy metal removal. Along with the development of genetic engineering and nano self-assembly technology, the self-assembly protein, functional polypeptide and other functional materials have strong application prospects in the fields of tissue engineering, drug loading, release, environmental repair and the like.
Therefore, how to provide a self-assembled nano-chelating peptide for removing heavy metal pollution by the bio-adsorption of functional polypeptides is a problem to be solved by those skilled in the art.
Disclosure of Invention
The invention aims to provide a multivalent nano chelating peptide and application thereof. The invention utilizes the genetic engineering technology to integrate self-assembled nano microglobulin ferritin gene rHF and artificial plant chelating peptide gene ECs with strong adsorption capacity to heavy metals, constructs multivalent nano chelating peptide rHF-L-ECs with heavy metal removal function in escherichia coli by taking escherichia coli as host cells, and is used for high-efficiency adsorption of heavy metals.
In order to achieve the above object, the present invention provides the following technical solutions:
The invention provides a multivalent nanometer chelating peptide, which comprises transferrin, a connecting peptide and an artificial plant chelating peptide which are sequentially connected; the amino acid sequence of the transferrin is shown as SEQ ID NO:1 is shown in the specification; the amino acid sequence of the artificial plant chelating peptide is shown as SEQ ID NO: 2.
Preferably, the amino acid sequence of the connecting peptide is shown in SEQ ID NO: 3.
Preferably, the amino acid sequence of the multivalent nano chelating peptide is shown in SEQ ID NO: 4.
Preferably, the nucleotide sequence of the multivalent nano chelating peptide is shown in SEQ ID NO: shown at 8.
The invention also provides a biological material containing the multivalent nano chelating peptide, wherein the biological material is an expression vector or engineering bacteria.
Preferably, the engineering bacteria are one of escherichia coli, yeast, blue algae, green algae and bacillus.
The invention also provides application of the multivalent nano chelating peptide or the biological material in heavy metal pollution remediation.
Preferably, the heavy metal is one or more of Cd2+、Cd4+、Ag+、Cr3+、Ni2+、U6+、Te3+、Co2+、Se6+、Pu3+、Hg2+、Mn2+、Zn2+ and Cu 2+.
Preferably, the method for repairing heavy metal pollution comprises the following steps: and mixing and adsorbing the engineering bacteria expressing the multivalent nano chelating peptide with the heavy metal solution.
Preferably, the final concentration of heavy metal in the heavy metal solution is 50-200 mg/L.
Preferably, the adsorption time is 1-3 hours;
preferably, the temperature during adsorption is set to be 20-40 ℃;
preferably, the pH at the time of adsorption is set to 5 to 8.
The rHF gene provided by the invention is a human iron transport protein heavy chain (rHF). Because of their unique structure and stable properties, they have been widely used as building blocks for the design and synthesis of a variety of nanoelement devices. Human ferritin rHF has the main functions of storing and dynamic balance control of iron element in vivo and has highly conserved biochemical and structural characteristics. As shown in FIG. 8 (A) rHF subunits, consisting of 180 amino acids, are spontaneously assembled in cells to form 24-mer nanocapsule structures with an outer diameter of 12 nm and an inner diameter of 8nm (B), the N-terminus of each subunit being exposed outside the cage. The overall spatial structure exhibits 4-3-2 symmetry. Ferritin has extremely high thermal stability, the Tm value can reach 85 ℃, the soluble expression and self-assembly can be realized in different hosts, and the amino acid sequence is shown as SEQ ID NO:1 is shown in the specification; the nucleotide sequence is shown in SEQ ID NO: shown at 5.
The invention designs a segment of repeated 20 times Glu-Cys repeated dipeptide structural units, the nucleic acid sequence of which is artificially synthesized, and the carboxyl of the glutamic acid and the sulfhydryl of the cysteine of the peptide have the capability of efficiently adsorbing and chelating heavy metal ions. The amino acid sequence of the artificial plant chelating peptide ECs is shown in SEQ ID NO:2, the nucleotide sequence is shown as SEQ ID NO: shown at 6.
A linker peptide L sequence was present between the linker rHF and the ECs to facilitate presentation of the ECs on the surface of the rHF spheres. The amino acid sequence of the connecting peptide L is shown in SEQ ID NO:3, the nucleotide sequence is shown as SEQ ID NO: shown at 7.
In the present invention, the connecting peptide may also be (GGGGS) 2 (shown as SEQ ID NO: 14), (EAAAK) 2 (shown as SEQ ID NO: 15).
Compared with the prior art, the invention has the following beneficial effects:
1. The invention carries out recombination fusion on the coding genes of ECs and rHF through a DNA fragment of a coding proper linking peptide (linker) to obtain fusion genes rHF-ECs, constructs an expression vector pET28a-rHF-ECs, selects escherichia coli as a host cell to transform escherichia coli BL21 (DE 3), and obtains polyvalent nano-chelating peptide capable of chelating heavy metal ions through induction expression; and treating sewage containing heavy metal cadmium and lead by using escherichia coli cells capable of expressing the nano chelating peptide.
2. After the multivalent nano chelating peptide provided by the invention is transformed into engineering bacteria, the multivalent nano chelating peptide has higher capability of absorbing heavy metal ions and good selectivity and specificity, has good application value, and provides a new thought and a new technology for bioremediation of heavy metal polluted environments.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
FIG. 1 shows the auto-inducible expression of engineering bacterium BL21 (FLE) in example 2; 1 is the expression strain sediment; 2 is the expression strain supernatant;
FIG. 2 is a TEM image of the transmission electron microscope of example 2 rHF-L-ECs;
FIG. 3 is a graph showing the determination of the optimal temperature for heavy metal adsorption by the recombinant strain of example 3;
FIG. 4 is a determination of the optimal pH for heavy metal adsorption by the recombinant strain of example 3;
FIG. 5 is an adsorption curve of cadmium for the recombinant strain of example 3 and the control strain;
FIG. 6 is an adsorption curve of lead for the recombinant strain of example 3 and the control strain;
FIG. 7 is a schematic representation of the synthesis of multivalent nano-chelating peptides rHF-L-ECs of the invention;
FIG. 8 is a block diagram of transferrin.
Detailed Description
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
1. Cloning of the target transferrin gene, using plasmid pET-28-rHF (shown as SEQ ID NO: 9) stored in laboratory as template, and using sequence
SEQ ID NO.10:5’-GATCCATATGACGACCGCGTCCACC-3’
SEQ ID NO.11:5'-TACCGGATCCGGGCGCTCCCATCTTGCG-3' as primer;
the reaction system is as follows: 50. Mu.L of the reaction system was added with 2X Prime starMax. Mu.L of the forward primer, 2. Mu.L of the reverse primer, 2. Mu.L of the template and 19. Mu.L of ddH 2 O, respectively.
PCR reaction procedure: pre-denaturation at 98℃for 2min, denaturation at 95℃for 10s, annealing at 55℃for 10s, extension at 72℃for 10s,30 cycles, and extension at 72℃for 2min.
The DNA fragment of the target transferrin gene rHF is obtained.
2. And (3) obtaining target gene ECs, carrying out gene sequence fusion on the connecting peptide sequence L and the target gene ECs, carrying out codon optimization, and delivering the sequences to a Jinsri biotechnology Co-Ltd for gene total synthesis, wherein enzyme cutting sites of BamHI and Xho I are respectively added at the 5 'and 3' ends of the synthesized sequences, so that the subsequent construction of recombinant expression vectors is facilitated. The synthesized gene was ligated into the cloning vector pUC57, designated pUC57-L-ECs (shown as SEQ ID NO: 12).
3. Construction of recombinant expression vector pET-28a (+) vector was digested with NdeI and Xho I recognition sites (recognition sequences), pET-28a (+) vector fragment was recovered after the digestion, PCR fragment of rHF gene amplified in the above step 1 was digested with restriction enzymes NdeI and BamHI, rHF gene fragment after the digestion was recovered, plasmid pUC57-L-ECs was digested with restriction enzymes BamHI and Xho I, and fragment-L-ECs was recovered.
The recombinant expression vector pET-28a (+) -rHF-L-ECs (shown as SEQ ID NO: 13) is obtained by connecting the digested and recovered pET-28a (+) vector fragment, rHF gene fragment and L-ECs fragment by using T4DNA LIGASE, and the recombinant plasmid is further verified by DNA sequencing to show that the vector construction is correct.
The recombinant expression vector pET-28a (+) -rHF-L-ECs, the coded protein sequence (sequence) includes the N-terminal vector tag sequence and 6xHis sequence besides the complete sequences of-rHF-and-L-ECs. If the first methionine is removed after expression, the expressed protein has a complete molecular weight (theory) of 30806Da.
4. The pET-28a (+) -rHF-L-ECs constructed in the step 3 are introduced into competent cells of escherichia coli BL21 (DE 3) by a chemical transformation method to obtain a recombinant strain containing a recombinant rHF-L-ECs gene, and the recombinant strain is named as E.coil BL21 (FLE).
Example 2
Expression of E.coil BL21 (FLE) engineering bacteria containing recombinant vector pET-28a (+) -rHF-L-ECs and optimization of expression conditions
1. The monoclonal E.coil BL21 (FLE) strain containing the recombinant vector pET-28a (+) -rHF-L-ECs obtained in example 1 was inoculated into LB liquid test tubes containing ampicillin at a ratio of 1%; placing in a shaking table at 37 ℃ and 150rpm for overnight culture for 17 hours to obtain test tube bacteria;
2. transferring the test tube bacterial liquid cultured overnight in the step 1 into 1L automatic induction liquid culture medium containing kanamycin sulfate according to the proportion of 5%; placing the strain in a shaking table at 37 ℃ and 150rpm for amplification culture for 16 hours to obtain a target strain containing recombinant proteins;
The automatic induction liquid culture medium comprises the following components: water is used as solvent, and the method comprises the following steps: 12g/L of tryptone, 5g/L of yeast extract, 50g/L of disodium hydrogen phosphate, 30g/L of sodium dihydrogen phosphate, 20g/L of ammonium chloride, 7.1g/L of sodium sulfate, 1g/L of magnesium sulfate, 20g/L of glucose, 100g/L of lactose and 100 mu g/L of kanamycin sulfate.
The target strain was crushed, centrifuged at 12000rpm for 15min to obtain supernatant and pellet, and SDS-PAGE was performed on the supernatant and pellet, respectively. As a result, FIG. 1 shows that rHF-L-ECs (FLE) successfully obtained soluble expression.
The assembly and identification of multivalent nano chelating peptide in cell, and the automatic induction expression of the fusion protein monomer rHF-L-ECs can spontaneously self-assemble in cell to form 24-polymer nano microsphere.
The supernatant obtained by crushing the cells in the step 2 was treated in a water bath at 60℃for 10 minutes, after other proteins were denatured, the supernatant was further precipitated with 20% ammonium sulfate, and after ultrafiltration to remove ammonium sulfate, the supernatant was dissolved again in 20mM Tris-HCl buffer, and the morphology of the target protein was observed by using a transmission electron microscope TEM, and the results are shown in FIG. 2: under the field of a TEM electron microscope, rHF-L-ECs can be observed to be successfully assembled into spherical nano particles, and the size is about 13-14 nm and is consistent with the theoretical size.
Example 3
Recombinant escherichia coli strain for treatment of heavy metals such as chromium chloride and lead chloride
1. Determination of optimum adsorption temperature
And (3) collecting thalli: the recombinant protein-containing target strain E.coil BL21 (FLE) obtained in step 2 of example 2 was placed in a 50mL centrifuge tube, centrifuged at 6000rpm for 5min, the cells were collected, 1g of the collected cells were resuspended in 100mL of distilled water, the cell density was about 10 10 cfu/mL, cdCl 2 at a final concentration of 50mg/L and PbCl 2 at a final concentration of 50mg/L were added, and the cells were sampled and examined after treatment at 150rpm for 2 hours under culture conditions of 16℃at 25℃at 37℃at 45 ℃. Digesting bacteria by microwaves, removing impurities, and measuring the metal content by an atomic absorption spectrometry or an inductively coupled plasma mass spectrometry; and evaluating the adsorption capacity of the bacterial cells.
The results are shown in FIG. 3: the E.coil BL21 (FLE) has the highest adsorption efficiency to CdCl 2 and PbCl 2 at 37 ℃, and is secondly at room temperature of 25 ℃ and is unfavorable for the adsorption of heavy metal particles at low temperature of 16 ℃.
2. Determination of the optimal adsorption pH
The collection of the cells was carried out in the same manner as in step 1, 1g of the collected cells was resuspended in Tris-HCl buffer solution having pH=5, 6, 7, 8, 9, cdCl 2 and PbCl 2 were added at a final concentration of 50mg/L, and the cells were sampled and detected after treatment at 37℃for 2 hours at 150 rpm. And (3) evaluating the adsorption capacity of the thalli by the detection method in step 1.
The results are shown in FIG. 4: under the reaction condition of pH 6-8, E.coil BL21 (FLE) has better adsorption efficiency on CdCl 2 and PbCl 2, wherein the optimal condition is that lead ions are pH=6, and cadmium ions are optimal pH=7.
3. Adsorption of CdCl 2 and PbCl 2 by recombinant Strain E.coil BL21 (FLE)
And (3) collecting thalli: culturing E.coil BL21 (FLE) -and control blank strain (strain without rHF-L-ECs) BL21 (DE 3), placing the bacterial liquid in a 50mL centrifuge tube, centrifuging at 6000rpm for 5min, collecting bacterial cells, taking 1g of the collected bacterial cells, re-suspending with distilled water, adding CdCl 2 and PbCl 2 with the final concentration of 50mg/L, treating at 37 ℃ at 150rpm for 2h, centrifuging at 6000rpm for 5min, washing the centrifugally collected bacterial cells with distilled water for three times, placing the bacterial cells in a 65 ℃ oven for 12h, and weighing dry weight (CDW) of the bacterial cells after fully drying the bacterial cells;
Metal content determination: digesting the dried bacteria by microwaves, removing impurities, and measuring the metal content by an atomic absorption spectrometry;
And (3) evaluating the adsorption capacity of the thalli: adsorption capacity (mg/g CDW) =total metal content (mg)/dry weight of cells (g)
The results are shown in fig. 5 and 6: when the adsorption equilibrium state of E.coil BL21 (FLE) was reached, compared with the adsorption equilibrium state of control strain BL21 (DE 3), the adsorption rate of E.coil BL21 (FLE) was 62.78%, the adsorption efficiency was improved by about 12.58% by comparison with 50.2% for the control strain, and the maximum adsorption amount of E.coil BL21 (FLE) on lead was calculated to be 94.17mg/g dry weight cells. The adsorption of cadmium by coil BL21 (FLE) was 81.8% and the control strain was 66.3%. The adsorption efficiency of E.coil BL21 (FLE) is improved by about 15.5 percent, and the maximum adsorption amount of E.coil BL21 (FLE) to cadmium is calculated to be 122.7mg/g dry weight cells.
The experimental results of the application of the embodiment show that the multivalent nano chelating peptide engineering bacteria obtained by the construction method provided by the invention can successfully self-assemble in escherichia coli cells to form spherical nano particles, and after the recombinant strain BL21 (FLE) is induced to express for 20 hours at 37 ℃, the recombinant strain BL21 (FLE) has higher adsorption capacity and good selectivity, has good application value, and provides a new thought and a new technology for bioremediation of heavy metal polluted environment.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (8)
1. A multivalent nano-chelating peptide, which is characterized by comprising transferrin, a connecting peptide and an artificial plant chelating peptide which are connected in sequence; the amino acid sequence of the transferrin is shown as SEQ ID NO:1 is shown in the specification; the amino acid sequence of the artificial plant chelating peptide is shown as SEQ ID NO:2 is shown in the figure;
The amino acid sequence of the connecting peptide is shown as SEQ ID NO: 3.
2. A biomaterial comprising the multivalent nano-chelating peptide according to claim 1, wherein the biomaterial is an expression vector or an engineering bacterium.
3. The biomaterial of claim 2, wherein the engineered bacterium is one of escherichia coli, yeast, cyanobacteria, green algae, and bacillus.
4. Use of a multivalent nano-chelating peptide according to claim 1 or a biomaterial according to claim 2 or 3 for the remediation of heavy metal pollution.
5. The use according to claim 4, wherein the heavy metal is Cd 2+ or Pb 2+.
6. The use according to claim 4 or 5, wherein the method for repairing heavy metal pollution is as follows: and mixing and adsorbing the engineering bacteria expressing the multivalent nano chelating peptide with the heavy metal solution.
7. The use according to claim 6, wherein the concentration of heavy metals in the heavy metal solution is 50-200 mg/L.
8. The use according to claim 7, wherein the time of adsorption is 1-3 hours; the temperature during adsorption is set to be 20-40 ℃; and the pH value during adsorption is set to be 5-8.
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