CN117451630A - Chemiluminescent light-gathering test tube applied to in-vitro diagnosis and manufacturing method thereof - Google Patents

Chemiluminescent light-gathering test tube applied to in-vitro diagnosis and manufacturing method thereof Download PDF

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Publication number
CN117451630A
CN117451630A CN202311174273.3A CN202311174273A CN117451630A CN 117451630 A CN117451630 A CN 117451630A CN 202311174273 A CN202311174273 A CN 202311174273A CN 117451630 A CN117451630 A CN 117451630A
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light
test tube
base
chemiluminescent
shell
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CN202311174273.3A
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Inventor
张建新
余婷
杨希峰
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Suzhou Yuxun Biotechnology Co ltd
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Suzhou Yuxun Biotechnology Co ltd
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Priority to CN202311174273.3A priority Critical patent/CN117451630A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F30/00—Computer-aided design [CAD]
    • G06F30/20—Design optimisation, verification or simulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0325—Cells for testing reactions, e.g. containing reagents

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Theoretical Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Plasma & Fusion (AREA)
  • Computer Hardware Design (AREA)
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Abstract

The invention discloses a chemiluminescent condensing test tube applied to in-vitro diagnosis, which comprises a light source and a reflecting test tube, wherein a condensing shell is arranged outside the reflecting test tube, and liquid is contained in the reflecting test tube; the position of the tube orifice of the reflecting test tube is provided with a receiver; the light source is spread outwards, light is concentrated to a test tube port through liquid, a test tube is reflected, then the light is concentrated through the reflection of the light-gathering shell, and finally the light is focused on the receiver.

Description

Chemiluminescent light-gathering test tube applied to in-vitro diagnosis and manufacturing method thereof
Technical Field
The invention relates to the field of chemiluminescence, in particular to a chemiluminescent condensing test tube applied to in-vitro diagnosis and a manufacturing method thereof.
Background
Chemiluminescence is a highly sensitive and specific analytical method, which can detect micro-substances such as pathogens, and can improve the specificity judgment of certain serological indexes to a certain extent for diagnosing diseases such as tumors which lack specificity.
The chemiluminescent condensing test tube is one of the commonly used detection methods in-vitro diagnosis, and the detection sensitivity and the signal-to-noise ratio are improved by focusing the optical signals generated by chemical reaction, so that the concentration change of the substance to be detected is detected more accurately. Since the function of the chemiluminescent light gathering test tube is very critical, the chemiluminescent light gathering test tube has important significance and purpose for design and research thereof. The chemiluminescent condensing test tube can improve the sensitivity and signal to noise ratio of detection. In some low concentration detection and micro sample detection, the conventional cuvette design may have signal loss and influence of the optical path length on the measurement result, so that the detection result is not accurate and sensitive enough. And the chemiluminescent light gathering test tube can weaken the interaction with the test tube or the interaction of the species to be measured, thereby realizing the improvement of the signal-to-noise ratio. Test errors may be caused by variations in some of the test conditions, such as optical path length, cuvette refractive index, etc. However, in the condensing test tube, the optical path length can be limited to a very short range, and meanwhile, the interaction and collision between the tested object and the test tube can be eliminated, so that the test precision is improved, and the error is reduced. Through reasonable design and optimization, the chemiluminescent light-gathering test tube can optimize the chemiluminescent determination method.
In practical application, due to the influence of factors such as small volume of a tested body, uncertain substrate dilution degree and the like, a chemiluminescent signal is often limited, a weak light signal entering a detector brings higher requirements on the detector, and the chemiluminescent emergence rate of a conventional U-shaped test tube is only 10%, so that the diagnostic sensitivity is not high.
Disclosure of Invention
The embodiment of the application solves the problems that the traditional test tube design in the prior art has signal loss and the optical path length has influence on the measurement result by providing the chemiluminescent condensing test tube applied to in-vitro diagnosis, so that the detection result is inaccurate and sensitive, and the signal to noise ratio is improved.
The embodiment of the application provides a chemiluminescent condensing test tube applied to in-vitro diagnosis. The liquid collecting device comprises a light source and a reflecting test tube, wherein a light collecting shell is arranged outside the reflecting test tube, and liquid is contained in the reflecting test tube; the position of the tube orifice of the reflecting test tube is provided with a receiver; the light source is spread outwards, light is collected to a test tube port through liquid, a test tube is reflected, then the light is reflected by the light-gathering shell, and finally the light is focused on the receiver.
Preferably, the base uses a focusing function to optimize the concave curvature of the base, and by changing the curvature of the base, the light incident on the base can be focused to the center of the receiver by reflection.
Preferably, the light source property is set to a reflectivity of 25% and a transmissivity of 75%.
Preferably, the radius of the light source is set to 2.999 mm and the light with the wavelength of 550 nm is used, the emergent angle of the light source is set to be vertical upwards, and the divergence angle is set to be 180 degrees.
Preferably, the mount is made of a polynomial aspheric body and the inner diameter in the base lens is set to 9.6094 mm.
Preferably, the mirror is optimized to have an input size of 6 mm and an overall length of 10 mm.
Preferably, the curvature of the base in the light-gathering housing is greater than the diameter of the bottom of the reflecting test tube.
Preferably, the chemiluminescent condensing test tube for in-vitro diagnosis is made of glass with a transmittance of 95%.
The embodiment of the application also provides a manufacturing method of the chemiluminescent condensing test tube applied to in-vitro diagnosis, which comprises the following steps:
step S01: setting light source attributes, adopting optical simulation software to simulate calculation, and setting the radius, emergent angle, divergence angle, reflectivity and transmissivity of a light source part;
step S02: setting properties of a condensing shell, and setting the input size, the output angle, the input angle and the overall length of the condensing shell as variables;
step S03: parameter optimization is carried out on the input size and the overall length of the condensing shell, constraint conditions of the input size and the overall length are set, the overall length and the input size are optimized through calculation, light rays which are scattered to the periphery in the light source are concentrated to a test tube port through the reflection attribute of the shell, and the input size and the overall length data of the condensing shell at the moment are recorded;
step S04: setting a receiver, wherein the size of the receiver corresponds to the size of the test tube port, and receiving the light rays emitted by the light source;
step S05: focusing optimization is performed on the base of the light-focusing shell, the option of adding an evaluation function and a focusing function is found in software, the curvature of the base in the light-focusing shell is set as a variable, and light rays entering the base can be focused to the center of the receiver through reflection through the change of the curvature in the focusing optimization process.
One or more technical solutions provided in the embodiments of the present application at least have the following technical effects or advantages:
1. due to the adoption of the light-gathering shell, the light rays transmitted out of the test tube are reflected back into the test tube by the principle of total reflection according to the characteristic of total reflection.
2. By optimizing the curvature of the base, the light on the base is focused to the center of the receiver to the greatest extent according to different angle changes by means of the diffuse reflection principle on the light reflecting base.
3. The light-gathering shell and the base have the curvature optimization effect, so that the light receiving rate reaches more than 30%.
Drawings
FIG. 1 is a schematic structural diagram of a chemiluminescent light collection tube of the present application for in vitro diagnostics;
FIG. 2 is a schematic diagram of a reflective cuvette structure according to the present application;
FIG. 3 is a schematic diagram of the total reflection phenomenon of the present application;
FIG. 4 is a schematic view of the light condensing housing structure of the present application;
FIG. 5 is a schematic view of the base structure of the light condensing housing of the present application;
FIG. 6 is a schematic diagram of the diffuse reflection phenomenon of the present application;
FIG. 7 is a schematic view of a light source structure of the present application;
FIG. 8 is a graph of forward illumination at a receiver of the present application;
FIG. 9 is a power diagram of the receiver when focusing the cuvette;
FIG. 10 is a plot of receiver power after focus function optimization using the focus tube of the present application;
fig. 11 is a flow chart of steps of the method of making the present application.
Detailed Description
In order to better understand the above technical solutions, the following detailed description will refer to the accompanying drawings and specific embodiments.
Example 1
As shown in fig. 1, the present application provides a chemiluminescent condensing tube for in vitro diagnosis, comprising a light source and a reflecting tube; a condensing shell is arranged outside the reflecting test tube, and as shown in fig. 2, liquid is contained in the reflecting test tube; the position of the tube orifice of the reflecting test tube is provided with a receiver; the light source propagates outward, concentrates light to the cuvette port via liquid, reflecting cuvette, then reflecting off the light-gathering housing, and finally focuses on the receiver.
The condensing shell and the reflecting test tube are important construction parts of experiments, and various geometrical optical principles are involved, wherein the principle of total reflection is the most important: as shown in fig. 2, when light is emitted from an optically dense medium to an optically sparse medium, in general, a reflection phenomenon and a refraction phenomenon occur simultaneously. The angle of refraction is greater than the angle of incidence, and increases as the angle of incidence increases. When the angle of incidence increases to a certain extent, i.e. the critical angle, the angle of refraction is equal to 90 °, and if the angle of incidence is increased again, the light rays will be totally reflected back into the original medium, a phenomenon known as total reflection of light. As can be seen from the above, the conditions necessary for total reflection are that light is directed from the optically dense medium to the optically sparse medium and that the angle of incidence is greater than the critical angle. The characteristic that this application was mainly passed through spotlight shell total reflection with the light that test tube transmitted out through the principle of total reflection with its reflection back inside the test tube.
As shown in fig. 3, the light-gathering shell is composed of a base and a reflecting mirror, and as shown in fig. 4, the part of the base facing the bottom of the test tube is designed to be concave by adopting a polynomial aspheric shape, so that different curved surface coefficients can be corresponding to different curvatures, and the light can be reflected to the receiver by changing the curved surface shape when the light-gathering shell is optimized.
The principle of the optimization part is mainly based on the diffuse reflection principle, as shown in fig. 5, which is a phenomenon that light rays incident on a rough surface emit light in various directions. The normal directions of the points on the rough surface are different, so even if the incident light is parallel light, the light rays which are reflected at different points on the surface are emitted in different directions according to the law of reflection, and the light rays which are reflected onto the base are focused to the center of the receiver to the greatest extent according to different angle changes by optimizing the curvature on the reflective base by means of the diffuse reflection principle.
As shown in fig. 6, the light source part sets the radius of the light source to 2.999 mm and uses light with a wavelength of 550 nm and sets the light source emergent angle to be vertical upwards and the divergence angle to be 180 degrees, because the invariance of liquid refraction is that dynamic water is regarded as static solid water, a semicircular model is used for replacing static water and endowing the static water with the characteristics of the light source of the object, and the optical property of the light source is changed into the optical property of water, namely, the reflectivity of 25 percent and the transmissivity of 75 percent.
The condensing shell part consists of a base and a reflecting mirror, wherein the base is made of a polynomial aspheric body, the inner diameter of the base lens is set to 9.6094 mm, the reflecting mirror part uses a reflecting mirror model of the light tools software to set the input size, the output angle and the input angle as variables, and constraint conditions are set for the input size and the overall length. The input size is changed into 6 mm and the total length is changed into 10 mm through the self-contained optimization formula of the system, and the system has the function of collecting the light rays which are scattered around in the light source to the test tube port through the reflection attribute of the shell; the cuvette section consists of two geometrical figures with glass as optical properties.
To receive light from the light source, we choose to place a virtual surface in the LightTools software to place the light receiver, the size of which corresponds to the size of the reflector mouth and set the grid distribution on the receiver to a 21 x 21 distribution, the number of light using 25000 pieces of LightTools software defaults to reference.
In the simulation of software, glass with the transmittance of 95% is adopted to manufacture a test tube model, a geometric body with the transmittance of 75% is adopted to replace a light source model, and through setting the optical properties, the preview ray trend of the real condensing test tube which is relatively close to an ideal type can be obtained in the simulation.
For the model placed in the LightTools software, variables and constraint conditions of a reflector are all deleted to avoid influencing subsequent focusing optimization, then options for adding an evaluation function and a focusing function are found in the LightTools software, and the curvature setting variables of a base in a condensing shell enable light rays entering the base to be focused to the center of a receiver through reflection through change of curvature in the focusing optimization process. After the optimization selection work in the current period is finished, clicking an optimization option in the LightTools software, and after the system optimization is finished, displaying a forward illuminance diagram on a receiver of the LightTools software as shown in FIG. 8.
When the optimization constraint is set for the inner diameter of the base of the condensing shell, the fact that the curvature of the whole base cannot be smaller than the diameter of the bottom of the test tube because of the aspheric surface of the base is considered, otherwise, the model change generated by optimization can be intersected with the bottom of the test tube, so that software is wrongly reported, the failure of the optimization process is caused, even software breakdown is caused, and the computer is dead.
As shown in fig. 9, the power diagram of the receiver in the light-condensing test tube shows that the intensity distribution of the light intensity at different positions on the receiver is obtained by summing the obtained data through an excel table to obtain data about 10 watts; as shown in fig. 10, to set the focusing tube and optimize the focal length function, the power synthesis table shows that the intensity distribution of the light intensity at different positions on the receiver is about 42 watts by summing the obtained data through an excel table. The total power of the light source is 100 watts, and the total power is not changed, and the receiving rate is improved to 42% compared with 10% of the first non-addition light housing under the optimization of an external light-gathering housing and a focusing evaluation function.
Example 2
The application also provides a manufacturing method of the chemiluminescent condensing test tube applied to in-vitro diagnosis, as shown in fig. 11, comprising the following steps:
step S01: setting light source attributes, adopting optical simulation software to simulate calculation, and setting the radius, emergent angle, divergence angle, reflectivity and transmissivity of a light source part;
step S02: setting properties of a condensing shell, and setting the input size, the output angle, the input angle and the overall length of the condensing shell as variables;
step S03: parameter optimization is carried out on the input size and the overall length of the condensing shell, constraint conditions of the input size and the overall length are set, the overall length and the input size are optimized through calculation, light rays which are scattered to the periphery in the light source are concentrated to a test tube port through the reflection attribute of the shell, and the input size and the overall length data of the condensing shell at the moment are recorded;
step S04: setting a receiver, wherein the size of the receiver corresponds to the size of the test tube port, and receiving the light rays emitted by the light source;
step S05: focusing and optimizing the base of the Light condensing shell, finding an option of adding an evaluation function and a focusing function in Light Tools software, setting the curvature of the base in the Light condensing shell as a variable, and focusing the Light rays entering the base to the center of the receiver through reflection by changing the curvature in the focusing and optimizing process.
The embodiments of the present invention are all preferred embodiments of the present invention, and are not intended to limit the scope of the present invention in this way, therefore: all equivalent changes in structure, shape and principle of the invention should be covered in the scope of protection of the invention.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims (9)

1. The chemiluminescent light-gathering test tube comprises a light source and a reflecting test tube, wherein a light-gathering shell is arranged outside the reflecting test tube, and liquid is contained in the reflecting test tube; the position of the tube orifice of the reflecting test tube is provided with a receiver; the light source is spread outwards, light is collected to a test tube port through liquid, a test tube is reflected, then the light is reflected by the light-gathering shell, and finally the light is focused on the receiver.
2. The chemiluminescent light collection cuvette of claim 1 wherein the base uses a focusing function to optimize the concave curvature of the base such that light incident on the base can be focused by reflection to the center of the receiver by varying the curvature of the base.
3. The chemiluminescent light collection tube of claim 1 wherein the light source property is set to a reflectance of 25% and a transmittance of 75%.
4. The chemiluminescent tube of claim 1 wherein the light source has a radius of 2.999 mm and emits light at a wavelength of 550 nm, and wherein the light source has an exit angle of 180 degrees and an exit angle of vertical.
5. The chemiluminescent light collection tube of claim 2 wherein the base is formed of a polynomial aspheric body and the base lens has an inner diameter of 9.6094 mm.
6. The chemiluminescent light collection tube of claim 5 wherein the reflector is optimized to provide an input size of 6 mm and an overall length of 10 mm.
7. The chemiluminescent light collection tube of claim 1 wherein the curvature of the base in the light collection housing is greater than the diameter of the bottom of the reflective tube.
8. The chemiluminescent light collection tube of claim 1 for in vitro diagnosis is characterized in that the chemiluminescent light collection tube for in vitro diagnosis is made of glass with a transmittance of 95%.
9. The chemiluminescent light-gathering test tube manufacturing method applied to in-vitro diagnosis is characterized by comprising the following steps of:
step S01: setting light source attributes, adopting optical simulation software to simulate calculation, and setting the radius, emergent angle, divergence angle, reflectivity and transmissivity of a light source part;
step S02: setting properties of a condensing shell, and setting the input size, the output angle, the input angle and the overall length of the condensing shell as variables;
step S03: parameter optimization is carried out on the input size and the overall length of the condensing shell, constraint conditions of the input size and the overall length are set, the overall length and the input size are optimized through calculation, light rays which are scattered to the periphery in the light source are concentrated to a test tube port through the reflection attribute of the shell, and the input size and the overall length data of the condensing shell at the moment are recorded;
step S04: setting a receiver, wherein the size of the receiver corresponds to the size of the test tube port, and receiving the light rays emitted by the light source;
step S05: focusing optimization is performed on the base of the light-focusing shell, the option of adding an evaluation function and a focusing function is found in software, the curvature of the base in the light-focusing shell is set as a variable, and light rays entering the base can be focused to the center of the receiver through reflection through the change of the curvature in the focusing optimization process.
CN202311174273.3A 2023-09-12 2023-09-12 Chemiluminescent light-gathering test tube applied to in-vitro diagnosis and manufacturing method thereof Pending CN117451630A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311174273.3A CN117451630A (en) 2023-09-12 2023-09-12 Chemiluminescent light-gathering test tube applied to in-vitro diagnosis and manufacturing method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311174273.3A CN117451630A (en) 2023-09-12 2023-09-12 Chemiluminescent light-gathering test tube applied to in-vitro diagnosis and manufacturing method thereof

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CN117451630A true CN117451630A (en) 2024-01-26

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