CN117435862A - Method for calculating and expressing synergistic factors of bactericides - Google Patents
Method for calculating and expressing synergistic factors of bactericides Download PDFInfo
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- CN117435862A CN117435862A CN202210854205.0A CN202210854205A CN117435862A CN 117435862 A CN117435862 A CN 117435862A CN 202210854205 A CN202210854205 A CN 202210854205A CN 117435862 A CN117435862 A CN 117435862A
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 114
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 97
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000002195 synergetic effect Effects 0.000 title claims abstract description 19
- 239000000203 mixture Substances 0.000 claims abstract description 58
- 101710097943 Viral-enhancing factor Proteins 0.000 claims abstract description 46
- 230000003385 bacteriostatic effect Effects 0.000 claims abstract description 21
- 238000005259 measurement Methods 0.000 claims abstract description 4
- 230000002401 inhibitory effect Effects 0.000 claims description 17
- 230000003115 biocidal effect Effects 0.000 claims description 11
- 239000003139 biocide Substances 0.000 claims description 11
- 238000004364 calculation method Methods 0.000 claims description 9
- 230000008485 antagonism Effects 0.000 claims description 7
- 230000000996 additive effect Effects 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 3
- 239000011885 synergistic combination Substances 0.000 claims 9
- 239000000417 fungicide Substances 0.000 claims 6
- 230000000694 effects Effects 0.000 abstract description 5
- 230000003993 interaction Effects 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 description 6
- 239000000126 substance Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 150000001875 compounds Chemical group 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 239000003619 algicide Substances 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000003641 microbiacidal effect Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 235000010241 potassium sorbate Nutrition 0.000 description 1
- 239000004302 potassium sorbate Substances 0.000 description 1
- 229940069338 potassium sorbate Drugs 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
- G06F17/10—Complex mathematical operations
- G06F17/18—Complex mathematical operations for evaluating statistical data, e.g. average values, frequency distributions, probability functions, regression analysis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
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Abstract
The invention discloses a method for calculating and expressing a synergistic factor of a bactericide, which comprises the following steps: firstly, calculating the theoretical bacteriostatic concentration of the mixture; secondly, calculating the actual measurement antibacterial concentration of the mixture; thirdly, calculating a synergy factor; fourth, the synergistic factor of the bactericide is expressed. A method for calculating and expressing the synergistic factors of bactericides can evaluate the interaction of bactericides at any concentration and can be used for determining the optimal ratio of the lowest concentration of each component in the mixture to exert the optimal effect.
Description
Technical Field
The invention relates to the field of bactericides, in particular to a method for calculating and expressing a synergistic factor of a bactericide.
Background
The bactericide is also called biocide, bactericidal algicide, microbiocide, etc. and refers to a substance that can effectively control or kill microorganisms. The product can be used as disinfectant, antiseptic, and mildew inhibitor, and can be used in human body, food, cosmetics, paint, leather, building material, metal processing liquid, plastic, water treatment, etc.
The bactericide is often used in a compound form, and aims to expand the antibacterial spectrum and solve the problem of drug resistance caused by using a single bactericide. The antibacterial effect of the bactericide on microorganisms after being compounded is greater than the sum of the effects of the two bactericides used independently, and the synergistic effect is called. On the contrary, the antibacterial effect of the bactericide on microorganisms after being compounded is smaller than the sum of the effects of the two bactericides used independently, and the antibacterial effect is called antagonism. In addition, the antibacterial effect of the bactericide on microorganisms after being compounded is equal to the sum of the effects of the two bactericides used independently, and the sum is called additive effect.
The existing evaluation mode adopts a grading bacteriostasis concentration, and a grading bacteriostasis concentration (fractional inhibitory concentration, FIC) index is a common method for evaluating the interaction relationship between an independent bactericide and two bactericides. Methods can be seen in T.Jadavji, CG Prober and R Cheung, antimicrobial Agents and Chemotherapy 26,91 (1984) and CN 109640656A.
The fractional inhibitory concentration of the biocide is calculated as the minimum concentration of the biocide in the mixture that inhibits the target microorganism divided by the minimum concentration of the biocide alone that inhibits the growth of the microorganism. And then adding the graded inhibitory concentrations of the two bactericides to obtain the total graded inhibitory concentration index.
Namely:
FIC (component a) =mic (minimum inhibitory concentration of component a tested in mixture)/MIC (minimum inhibitory concentration of component a as a single component)
FIC (component B) =mic (minimum inhibitory concentration of component B tested in mixture)/MIC (minimum inhibitory concentration of component B as a single component)
Σfic=fic (component a) +fic (component B)
The interaction between antimicrobial combinations can be judged according to the magnitude of the value of Σfic. The Sigma FIC is <1, and the two antibacterial substances have synergistic effect; Σfic=1, and the two antibacterial substances are added as bactericides; sigma FIC > 1, and the two antibacterial substances are in antagonistic relationship. The smaller the grading antibacterial concentration index obtained by the method is, the more obvious the synergistic effect is. However, this method only calculates the fractional inhibitory concentration index of the minimum inhibitory concentration data point of the mixture, and cannot obtain the synergistic index of each composition with different concentrations, and cannot determine the optimal ratio of the two bactericides.
Thus, there is a need to devise a method for calculating and expressing the synergistic factors of bactericides.
Disclosure of Invention
To overcome the deficiencies in the prior art, a method for calculating and expressing a synergistic factor of a biocide is provided.
The invention is realized by the following scheme:
a method for calculating a synergistic factor of a bactericide, the method comprising the steps of:
first, calculating the theoretical bacteriostatic concentration of the mixture: wherein the theoretical bacteriostatic concentration of the mixture is expressed by IC (theoretical value), and the calculation method of the IC (theoretical value) is as follows:
wherein: a and B respectively represent two different bactericides, a and B are the proportions of the two bactericides A and B in different combinations of mixtures, and MIC (A) and MIC (B) are the minimum antibacterial concentrations of the two bactericides A and B when the two bactericides A and B are used independently;
secondly, calculating the actual measurement antibacterial concentration of the mixture: wherein the actual measured bacteriostatic concentration of the mixture is expressed by IC (actual measured value), and the calculation method of the IC (actual measured value) is as follows:
IC (actual value) =ic (CA) +ic (CB)
Wherein: IC (CA) and IC (CB) are respectively the concentrations obtained by actual testing of the two bactericides A and B when the bactericides A and B are used in combination, namely the mixtures of the two bactericides A and B in different combinations;
thirdly, calculating a synergy factor: wherein the synergistic factor of the bactericide is expressed by SI (synergistic factor), and the calculation method of the SI (synergistic factor) is as follows:
in the first step, a divided by MIC (a) represents the percentage of biocide a required per unit of bacteriostatic concentration in the various combination mixtures.
Dividing B by MIC (B) represents the percentage of biocide B in the mixture required per unit of bacteriostatic concentration.
In the first step, the IC (theoretical value) is equal to the sum of the total content of bactericides a and B in the different combination mixtures divided by the percentage of content of bactericides a and B required per unit of bacteriostatic concentration.
In the second step, the IC (measured value) is equal to the sum of the concentrations of the two bactericides A and B actually tested when used in combination in the combination mixture.
In the third step, the SI (synergistic factor) is the ratio between the theoretical inhibitory concentration IC (theoretical value) of the mixture and the actual inhibitory concentration IC (actual value) of the mixture.
A method for expressing a bactericide synergy factor, wherein the bactericide synergy factor is expressed by SI (synergy factor), and if the numerical value of SI (synergy factor) is larger, the larger the synergy is expressed; the smaller the SI (synergistic factor), the greater the antagonism.
If SI (synergistic factor) is greater than 1, it means that there is a synergistic effect between the two bactericides;
if SI (synergistic factor) is equal to 1, this means that there is a additive effect between the two bactericides;
if the SI (synergistic factor) is less than 1, antagonism is indicated between the two bactericides.
SI (synergistic factor) can obtain the synergistic effect of two bactericides in a mixture of different combinations, and the numerical value of SI (synergistic factor) is used for representing the synergistic effect of two bactericides in a mixture of different combinations.
The optimal proportion of the two bactericides in the mixture of different combinations can be determined by using SI (synergistic factor), and the proportion of the bactericides in the mixture when SI (synergistic factor) is maximum is taken as the optimal proportion.
The beneficial effects of the invention are as follows:
a method for calculating and expressing the synergistic factors of bactericides can evaluate the interaction of bactericides at any concentration and can be used for determining the optimal ratio of the lowest concentration of each component in the mixture to exert the optimal effect.
Drawings
FIG. 1 is a flow chart of a method for calculating a synergistic factor of a bactericide according to the present invention.
Detailed Description
Preferred embodiments of the present invention will be further described with reference to the accompanying drawings:
as shown in fig. 1, a method for calculating a synergistic factor of a bactericide, the method comprising the steps of:
first, calculating the theoretical bacteriostatic concentration of the mixture: wherein the theoretical bacteriostatic concentration of the mixture is expressed by IC (theoretical value), and the calculation method of the IC (theoretical value) is as follows:
wherein: a and B respectively represent two different bactericides, a and B are the proportions of the two bactericides A and B in different combinations of mixtures, and MIC (A) and MIC (B) are the minimum antibacterial concentrations of the two bactericides A and B when the two bactericides A and B are used independently;
secondly, calculating the actual measurement antibacterial concentration of the mixture: wherein the actual measured bacteriostatic concentration of the mixture is expressed by IC (actual measured value), and the calculation method of the IC (actual measured value) is as follows:
IC (actual value) =ic (CA) +ic (CB)
Wherein: IC (CA) and IC (CB) are respectively the concentrations obtained by actual testing of the two bactericides A and B when the bactericides A and B are used in combination, namely the mixtures of the two bactericides A and B in different combinations;
thirdly, calculating a synergy factor: wherein the synergistic factor of the bactericide is expressed by SI (synergistic factor), and the calculation method of the SI (synergistic factor) is as follows:
in the first step, a divided by MIC (a) represents the percentage of biocide a required per unit of bacteriostatic concentration in the various combination mixtures.
Dividing B by MIC (B) represents the percentage of biocide B in the mixture required per unit of bacteriostatic concentration.
In the first step, the IC (theoretical value) is equal to the sum of the total content of bactericides a and B in the different combination mixtures divided by the percentage of content of bactericides a and B required per unit of bacteriostatic concentration.
In the second step, the IC (measured value) is equal to the sum of the concentrations of the two bactericides A and B actually tested when used in combination in the combination mixture.
In the third step, the SI (synergistic factor) is the ratio between the theoretical inhibitory concentration IC (theoretical value) of the mixture and the actual inhibitory concentration IC (actual value) of the mixture.
A method for expressing a bactericide synergy factor, wherein the bactericide synergy factor is expressed by SI (synergy factor), and if the numerical value of SI (synergy factor) is larger, the larger the synergy is expressed; the smaller the SI (synergistic factor), the greater the antagonism.
If SI (synergistic factor) is greater than 1, it means that there is a synergistic effect between the two bactericides;
if SI (synergistic factor) is equal to 1, this means that there is a additive effect between the two bactericides;
if the SI (synergistic factor) is less than 1, antagonism is indicated between the two bactericides.
SI (synergistic factor) can obtain the synergistic effect of two bactericides in a mixture of different combinations, and the numerical value of SI (synergistic factor) is used for representing the synergistic effect of two bactericides in a mixture of different combinations.
The optimal proportion of the two bactericides in the mixture of different combinations can be determined by using SI (synergistic factor), and the proportion of the bactericides in the mixture when SI (synergistic factor) is maximum is taken as the optimal proportion.
The technical scheme of the application is further described below in conjunction with the specific mode:
the minimum inhibitory concentration of sodium benzoate and potassium sorbate was determined for the mixtures of different combinations using tryptone soy broth. 1-5×10 6 Coli was inoculated into a liquid medium (pH 5) containing the corresponding bactericide, and after incubation at 37℃for 24 hours, the growth of the cells was observed by liquid turbidity, MIC was defined as the minimum concentration at which no cells grew, and the specific results are shown in Table 1. As can be seen from table 1, the novel method for calculating and expressing the synergistic factor of the bactericide disclosed by the invention can be used for screening the optimal proportion of the compound components of the bactericide and judging the action relationship between the two bactericides by calculating the ratio of the theoretical bacteriostatic concentration to the measured bacteriostatic concentration in the combination of the different concentrations of the two bactericides to determine the size of the synergistic factor.
Table 1: synergistic factors of different combinations of bactericides
While the invention has been described and illustrated in considerable detail, it should be understood that modifications and equivalents to the above-described embodiments will become apparent to those skilled in the art, and that such modifications and improvements may be made without departing from the spirit of the invention.
Claims (10)
1. A method for calculating a synergistic factor of a bactericide, the method comprising the steps of:
first, calculating the theoretical bacteriostatic concentration of the mixture: wherein the theoretical bacteriostatic concentration of the mixture is expressed by IC (theoretical value), and the calculation method of the IC (theoretical value) is as follows:
wherein: a and B respectively represent two different bactericides, a and B are the proportions of the two bactericides A and B in different combinations of mixtures, and MIC (A) and MIC (B) are the minimum antibacterial concentrations of the two bactericides A and B when the two bactericides A and B are used independently;
secondly, calculating the actual measurement antibacterial concentration of the mixture: wherein the actual measured bacteriostatic concentration of the mixture is expressed by IC (actual measured value), and the calculation method of the IC (actual measured value) is as follows:
IC (actual value) =ic (CA) +ic (CB)
Wherein: IC (CA) and IC (CB) are respectively the concentrations obtained by actual testing of the two bactericides A and B when the bactericides A and B are used in combination, namely the mixtures of the two bactericides A and B in different combinations;
thirdly, calculating a synergy factor: wherein the synergistic factor of the bactericide is expressed by SI (synergistic factor), and the calculation method of the SI (synergistic factor) is as follows:
2. a method for calculating a synergistic combination of fungicides as claimed in claim 1, wherein: in the first step, a divided by MIC (a) represents the percentage of biocide a required per unit of bacteriostatic concentration in the various combination mixtures.
3. A method for calculating a synergistic combination of fungicides as claimed in claim 1, wherein: dividing B by MIC (B) represents the percentage of biocide B in the mixture required per unit of bacteriostatic concentration.
4. A method for calculating a synergistic combination of fungicides as claimed in claim 1, wherein: in the first step, the IC (theoretical value) is equal to the sum of the total content of bactericides a and B in the different combination mixtures divided by the percentage of content of bactericides a and B required per unit of bacteriostatic concentration.
5. A method for calculating a synergistic combination of fungicides as claimed in claim 1, wherein: in the second step, the IC (measured value) is equal to the sum of the concentrations of the two bactericides A and B actually tested when used in combination in the combination mixture.
6. A method for calculating a synergistic combination of fungicides as claimed in claim 1, wherein: in the third step, the SI (synergistic factor) is the ratio between the theoretical inhibitory concentration IC (theoretical value) of the mixture and the actual inhibitory concentration IC (actual value) of the mixture.
7. A method of expressing a synergistic combination of fungicides as claimed in any one of claims 1 to 6, wherein: the bactericide synergy factor is expressed in terms of SI (synergy factor), and if the value of SI (synergy factor) is larger, the larger the synergy is expressed; the smaller the SI (synergistic factor), the greater the antagonism.
8. A method for expressing a synergistic combination of bactericides as claimed in claim 7, wherein: if SI (synergistic factor) is greater than 1, it means that there is a synergistic effect between the two bactericides;
if SI (synergistic factor) is equal to 1, this means that there is a additive effect between the two bactericides;
if the SI (synergistic factor) is less than 1, antagonism is indicated between the two bactericides.
9. A method for expressing a synergistic combination of bactericides as claimed in claim 1, wherein: SI (synergistic factor) can obtain the synergistic effect of two bactericides in a mixture of different combinations, and the numerical value of SI (synergistic factor) is used for representing the synergistic effect of two bactericides in a mixture of different combinations.
10. A method for expressing a synergistic combination of bactericides as claimed in claim 1, wherein: the optimal proportion of the two bactericides in the mixture of different combinations can be determined by using SI (synergistic factor), and the proportion of the bactericides in the mixture when SI (synergistic factor) is maximum is taken as the optimal proportion.
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| CN202210854205.0A CN117435862A (en) | 2022-07-14 | 2022-07-14 | Method for calculating and expressing synergistic factors of bactericides |
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| CN202210854205.0A CN117435862A (en) | 2022-07-14 | 2022-07-14 | Method for calculating and expressing synergistic factors of bactericides |
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