CN117348224A - Microscopic device - Google Patents
Microscopic device Download PDFInfo
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- CN117348224A CN117348224A CN202311641119.2A CN202311641119A CN117348224A CN 117348224 A CN117348224 A CN 117348224A CN 202311641119 A CN202311641119 A CN 202311641119A CN 117348224 A CN117348224 A CN 117348224A
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- 238000005286 illumination Methods 0.000 claims abstract description 75
- 238000000386 microscopy Methods 0.000 claims abstract description 24
- 230000005284 excitation Effects 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 abstract description 6
- 239000000523 sample Substances 0.000 description 69
- 239000012472 biological sample Substances 0.000 description 5
- 238000003384 imaging method Methods 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 238000000018 DNA microarray Methods 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- -1 antibody Proteins 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
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Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/12—Condensers affording bright-field illumination
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
Abstract
The invention provides a microscopic device, and relates to the technical field of optical instruments and equipment. The microscopy apparatus includes a fluorescent field illumination assembly, a bright field illumination assembly, an image acquisition assembly, and a dichroic mirror set. The fluorescence field illumination assembly is configured to excite fluorescence of the sample. The bright field illumination assembly is configured to illuminate the sample to provide a bright field to the sample. The image acquisition assembly is configured to acquire an image of the sample under illumination by the fluorescent field illumination assembly and/or the bright field illumination assembly. A dichroic mirror group configured to transmit the light beam emitted by the fluorescent field illumination assembly and/or bright field illumination assembly to the sample and to transmit the excited fluorescent and/or reflected light of the sample to the image acquisition assembly. The three images of the sample under the superposition of the bright field, the fluorescent field and the bright field and the fluorescent field can be realized, and the three images are more convenient to use, low in height and small in volume.
Description
Technical Field
The invention relates to the field of optical instruments and equipment, in particular to a microscopic device.
Background
Microscopic devices have been used to capture images of samples, such as biological samples, providing an important indicator for modern clinical testing and diagnosis.
However, the existing microscope device is simple, can only acquire a single functional image, and is inconvenient to use due to the fact that multiple devices are needed to cooperate.
Disclosure of Invention
The invention aims at providing a microscopic device which can realize three images of a sample under the superposition of a bright field, a fluorescent field and the bright field, and is more convenient to use.
Embodiments of the invention may be implemented as follows:
in a first aspect, the present invention provides a microscopy apparatus comprising:
a fluorescence field illumination assembly configured to excite fluorescence of a sample;
a bright field illumination assembly configured to illuminate the sample to provide a bright field to the sample;
an image acquisition assembly configured to acquire an image of a sample under illumination by the fluorescent field illumination assembly and/or the bright field illumination assembly; the method comprises the steps of,
a dichroic mirror group configured to transmit the light beam emitted by the fluorescent field illumination assembly and/or the bright field illumination assembly to the sample and to transmit the excited fluorescent and/or reflected light of the sample to the image acquisition assembly.
In an alternative embodiment, the dichroic mirror set comprises a first dichroic mirror and a second dichroic mirror;
the first dichroic mirror can enable light rays incident on the first incident side and the second incident side to be emitted from a first emergent side;
the second dichroic mirror is arranged on a first emergent side of the first dichroic mirror, and the light beam emitted through the first emergent side can be projected to a sample through the second dichroic mirror;
the second dichroic mirror may also project reflected light and/or excited fluorescence light of the sample to the image acquisition assembly.
In an alternative embodiment, the first dichroic mirror may refract the light beam incident in the first incident direction so as to pass through the first dichroic mirror and be emitted from the first emitting direction of the first dichroic mirror;
the first dichroic mirror can reflect the light beam incident in the second incident direction, so that the light beam passes through the first dichroic mirror and then is emitted from the first emergent direction of the first dichroic mirror.
In an alternative embodiment, the second dichroic mirror is parallel to the first dichroic mirror;
the light beam emitted from the first emergent direction of the first dichroic mirror can be reflected by the second dichroic mirror and can be projected to a sample;
the reflected light and/or excited fluorescence of the sample is refracted through the second dichroic mirror and is transmitted through the dichroic mirror to the image acquisition assembly.
In an alternative embodiment, the microscopy apparatus further comprises a sample holder;
the sample rack is arranged on one side of the second dichroic mirror and is used for carrying samples;
the sample holder, the second dichroic mirror, and the image acquisition assembly are located on a first axis;
the fluorescent field illumination assembly, the first dichroic mirror, and the second dichroic mirror are located on a second axis;
the first axis and the second axis are perpendicular, and the second dichroic mirror and the first axis and the second axis are both at 45 degrees.
In an alternative embodiment, the fluorescent field illumination assembly includes an excitation light source and a first filter;
the excitation light source is arranged on a first incident side of the first dichroic mirror, and the first optical filter is arranged between the first dichroic mirror and the excitation light source.
In an alternative embodiment, the bright field illumination assembly includes a light source and a second filter;
the light source is arranged on the second incident side of the first dichroic mirror, and the second optical filter is arranged between the first dichroic mirror and the light source.
In an alternative embodiment, the image acquisition assembly includes a camera, a corrective lens, and a third filter;
the camera, the correction lens and the third filter are sequentially arranged on one side of the second dichroic mirror.
In an alternative embodiment, the microscopy apparatus further comprises a lens group for adjusting magnification;
the lens group is arranged between the sample frame and the second dichroic mirror.
In an alternative embodiment, the microscopy apparatus further comprises a collection mirror;
the condenser lens is disposed between the first dichroic mirror and the second dichroic mirror.
The microscopic device provided by the embodiment of the invention has the beneficial effects that:
the present application configures a fluorescent field illumination assembly to excite fluorescence of a sample, a bright field illumination assembly configured to illuminate the sample to provide a bright field to the sample, and an image acquisition assembly configured to acquire an image of the sample under illumination by the fluorescent field illumination assembly and/or the bright field illumination assembly. A dichroic mirror group is provided configured to transmit the light beam emitted by the fluorescent field illumination assembly and/or the bright field illumination assembly to the sample and to transmit the excited fluorescent and/or reflected light of the sample to the image acquisition assembly. The light path system is simple, three images of the sample under the superposition of the bright field, the fluorescent field and the bright field and the fluorescent field can be realized, and the use is more convenient.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of an image acquisition assembly of a microscopy apparatus according to an embodiment of the present invention positioned on top of the microscopy apparatus;
fig. 2 is a schematic structural diagram of an image acquisition component of a microscope device according to an embodiment of the present invention at the bottom of the microscope device.
Icon: 100-microscopy apparatus; 110-a fluorescent field lighting assembly; 111-an excitation light source; 113-a first filter; 130-bright field lighting assembly; 131-a light source; 133-a second filter; 150-an image acquisition component; 151-a third filter; 153-corrective lenses; 155-a camera; 170-a dichroic mirror group; 173-a first dichroic mirror; 175-a second dichroic mirror; 180-lens group; 190-sample rack; 300-samples.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present invention, it should be noted that, if the terms "upper", "lower", "inner", "outer", and the like indicate an azimuth or a positional relationship based on the azimuth or the positional relationship shown in the drawings, or the azimuth or the positional relationship in which the inventive product is conventionally put in use, it is merely for convenience of describing the present invention and simplifying the description, and it is not indicated or implied that the apparatus or element referred to must have a specific azimuth, be configured and operated in a specific azimuth, and thus it should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like, if any, are used merely for distinguishing between descriptions and not for indicating or implying a relative importance.
It should be noted that the features of the embodiments of the present invention may be combined with each other without conflict.
Referring to fig. 1, the present embodiment provides a microscope apparatus 100 including a fluorescent field illumination assembly 110, a bright field illumination assembly 130, an image acquisition assembly 150, and a dichroic mirror assembly 170. The fluorescence field illumination assembly 110 is configured to excite fluorescence of the sample 300. The bright field illumination assembly 130 is configured to illuminate the sample 300 to provide a bright field to the sample 300. An image acquisition assembly 150 configured to acquire an image of the sample 300 under illumination by the fluorescent field illumination assembly 110 and/or bright field illumination assembly 130. Dichroic mirror set 170 is configured to transmit the light beam emitted by fluorescent field illumination assembly 110 and/or bright field illumination assembly 130 to sample 300 and to transmit the excited fluorescent and/or reflected light of sample 300 to image acquisition assembly 150.
The present embodiment configures the fluorescent field illumination assembly 110 to excite fluorescence of the sample 300, the bright field illumination assembly 130 is configured to illuminate the sample 300 to provide a bright field to the sample 300, and the image acquisition assembly 150 is configured to acquire an image of the sample 300 under illumination by the fluorescent field illumination assembly 110 and/or the bright field illumination assembly 130. A dichroic mirror group 170 is provided and configured to transmit the light beam emitted by the fluorescent field illumination assembly 110 and/or bright field illumination assembly 130 to the sample 300 and to transmit the excited fluorescent and/or reflected light of the sample 300 to the image acquisition assembly 150. The light path system is simple, three images of the sample 300 under the superposition of the bright field, the fluorescent field and the bright field and the fluorescent field can be realized, and the use is more convenient.
Note that the sample 300 observed by using the microscope apparatus 100 according to the present technology is not particularly limited. However, it is preferred that the sample 300 is a biological sample 300 obtained in vivo or in vitro, such as a sample 300 of biological tissue, cells or liquid origin. The biological sample 300 may be, for example, a sample 300 isolated from humans and other mammals, including body fluids (e.g., blood, serum, plasma, urine, semen, cerebrospinal fluid, saliva, sweat, tears, ascites, or amniotic fluid), cells, tissues, organs, and diluted solutions containing such a sample 300. Further, the sample 300 suitable for use in the present technology is not limited to a sample 300 derived from a mammal, and may be derived from a prokaryote or a eukaryote. Further, the sample 300 may be a biological sample 300 slice (e.g., an organ or tissue slice) comprising tissue or an extract (e.g., an antigen, antibody, protein, or nucleic acid) from the biological sample 300. Samples 300 suitable for use in the present technique may be stained with one or more fluorescent dyes.
Referring to fig. 1, in the present embodiment, the dichroic mirror set 170 includes a first dichroic mirror 173 and a second dichroic mirror 175. The fluorescent field illumination assembly 110 and the bright field illumination assembly 130 are respectively disposed on a first incident side and a second incident side of the first dichroic mirror 173, and the first dichroic mirror 173 can make light incident on the first incident side and the second incident side be emitted from the first emitting side. The second dichroic mirror 175 is disposed on the first exit side of the first dichroic mirror 173, and the light beam emitted through the first exit side can be projected to the sample 300 through the second dichroic mirror 175. Second dichroic mirror 175 may also project reflected light and/or excited fluorescence of sample 300 to image acquisition assembly 150.
In this embodiment, the fluorescent field illumination assembly 110 and the bright field illumination assembly 130 are respectively disposed on the first incident side and the second incident side of the first dichroic mirror 173, so that the excitation light and the bright field light can be emitted from the same direction by using the beam combining principle of the dichroic mirror, and the integration level is high. Most importantly, the height of the microscopy apparatus 100 can be reduced.
In this embodiment, the first dichroic mirror 173 may refract the light beam incident in the first incident direction so as to transmit through the first dichroic mirror 173 and be emitted from the first emitting direction of the first dichroic mirror 173. The first dichroic mirror 173 may reflect the light beam incident in the second incident direction, so that the light beam passes through the first dichroic mirror 173 and is emitted from the first emitting direction of the first dichroic mirror 173. The first dichroic mirror 173 and the second dichroic mirror 175 are parallel.
The light beams emitted from the fluorescent field illumination unit 110 and the bright field illumination unit 130 can be made to enter the second dichroic mirror 175 from the same direction by the first dichroic mirror 173. The light path is simpler.
Specifically, the second dichroic mirror 175 is parallel to the first dichroic mirror 173, and is disposed inclined at 45 ° each. The light beam emitted from the first emission direction of the first dichroic mirror 173 may be reflected by the second dichroic mirror 175 and may be projected to the sample 300. The reflected light and/or excited fluorescence of sample 300 is refracted through second dichroic mirror 175 to image acquisition assembly 150.
Thus, with one image acquisition component 150, images in two scenes can be acquired, and the cost is not greatly influenced under the condition of realizing functional integration.
The dichroic mirror may split or combine the light beams according to wavelength. The specific principle is that the dichroic mirror is coated with the filter film and the antireflection film on two sides respectively, so that the dichroic mirror presents high-transmittance or high-reflection property to light beams with different wavelengths in two incident directions, and is commonly used for splitting/combining light beams with different wavelengths. One of the first incident direction and the second incident direction is a high-transmittance direction of the dichroic mirror, and the other is a high-reflectance direction of the dichroic mirror.
Referring to fig. 1, in the present embodiment, the fluorescent field illumination device 110 is disposed in a high incident direction of the first dichroic mirror 173, and the bright field illumination device 130 is disposed in a high reverse incident direction of the first dichroic mirror 173. Of course, in other embodiments of the present application, the positions of the two may be interchanged, but the film layers on both sides of the dichroic mirror need to be adaptively replaced.
In this embodiment, the microscopy apparatus 100 further includes a sample holder 190. The sample holder 190 is disposed at one side of the second dichroic mirror, and the sample holder 190 is used to hold a sample 300. Sample holder 190, second dichroic mirror 175, and image acquisition assembly 150 are located on a first axis. Fluorescent field illumination assembly 110, first dichroic mirror 173, and second dichroic mirror 175 are located on a second axis. The first axis and the second axis are perpendicular, and the second dichroic mirror 175 is at an angle of 45 ° to both the first axis and the second axis.
Typically, the sample 300 is mounted on the sample rack 190 by a biochip, such as a slide.
The light path is arranged in this way in this embodiment, so that the height of the microscope device 100 can be lower, and thus the operation can be more convenient and the cost can be saved.
Of course, in other embodiments of the present application, first dichroic mirror 173 and second dichroic mirror 175 may also be perpendicular, with particular perpendicularity or parallelism thereof being configurable depending on the particular locations of bright field illumination assembly 130 and fluorescent field illumination assembly 110, and determinable depending on the locations thereof on either side of second dichroic mirror 175. The present embodiment is not particularly limited.
In this embodiment, the fluorescent field illumination assembly 110 includes an excitation light source 111 and a first filter 113. The excitation light source 111 is disposed on a first incident side of the first dichroic mirror 173, and the first filter 113 is disposed between the first dichroic mirror 173 and the excitation light source 111.
The embodiment is provided with the optical filter so as to filter stray light and enable imaging to be clearer.
Of course, in other embodiments of the present application, the dichroic mirror may be further used to superimpose the beam materials, and the excitation light source 111 capable of exciting different fluorescence may be added according to the requirement, so that the classification and classification statistics of the sample 300 may be facilitated, and the function of the sample 300 is more powerful.
Referring to fig. 1, in the present embodiment, the bright field illumination assembly 130 includes a light source 131 and a second filter 133. The light source 131 is disposed at the second incident side of the first dichroic mirror 173, and the second filter 133 is disposed between the first dichroic mirror 173 and the light source 131. The second filter 133 can filter stray light to make imaging clearer.
It should be noted that, the light source 131 may be a composite light source 131, such as an LED light source 131 that may emit white light. The light source 131 may also be a monochromatic LED light source 131, such as a red light source 131, a yellow light source 131, etc. Of course, the bright field illumination assembly 130 may also be configured to superimpose different monochromatic or composite light sources 131 via dichroic mirrors, thereby enabling the microscopy apparatus 100 to capture more images for different functions.
In the present embodiment, the image acquisition assembly 150 includes a camera 155, a correction lens 153, and a third filter 151. The camera 155, the correction lens 153, and the third filter 151 are sequentially disposed at one side of the second dichroic mirror 175. In this embodiment, the image of the microscope device 100 in three different light fields can be obtained by one camera 155, which is simple in structure and convenient to use.
Of course, in other embodiments of the present application, multiple cameras 155 may be provided, so that multiple images may be acquired with one adjustment, thereby making use more convenient.
Referring to fig. 1, in the present embodiment, the microscope apparatus 100 further includes a lens assembly 180 for adjusting magnification. The lens group 180 is disposed between the sample holder 190 and the second dichroic mirror 175. The lens group 180 is provided in this embodiment, so that the imaging size and imaging quality of the sample 300 can be conveniently adjusted, and the use is more convenient.
In this embodiment, the microscopy apparatus 100 further comprises a collection mirror. The condenser lens is disposed between the first dichroic mirror 173 and the second dichroic mirror 175. The condensing lens is arranged to realize condensing, so that the light energy utilization efficiency is improved.
In this embodiment, the microscope device 100 may further include a base optical element such as a light homogenizing sheet, which ensures uniformity of imaging.
Referring to fig. 1, in the present embodiment, a sample holder 190 is provided at the bottom of the microscope device 100, which facilitates mounting of the sample 300, while an image acquisition assembly 150 is mounted at the top of the microscope device 100 for ease of viewing. Of course, referring to FIG. 2, the sample holder 190 may also be positioned at the top and the image acquisition assembly 150 mounted at the bottom of the microscopy apparatus 100.
In summary, the working principle and beneficial effects of the microscope device 100 provided in the embodiment of the present invention include:
the present embodiment configures the fluorescent field illumination assembly 110 to excite fluorescence of the sample 300, the bright field illumination assembly 130 is configured to illuminate the sample 300 to provide a bright field to the sample 300, and the image acquisition assembly 150 is configured to acquire an image of the sample 300 under illumination by the fluorescent field illumination assembly 110 and/or the bright field illumination assembly 130. A dichroic mirror group 170 is provided and configured to transmit the light beam emitted by the fluorescent field illumination assembly 110 and/or bright field illumination assembly 130 to the sample 300 and to transmit the excited fluorescent and/or reflected light of the sample 300 to the image acquisition assembly 150. The light path system is simple, three images of the sample 300 under the superposition of the bright field, the fluorescent field and the bright field and the fluorescent field can be realized, and the use is more convenient. And the provision of dichroic mirror set 170 allows the height of microscopy apparatus 100 to be reduced, for more advantageous operation.
The present invention is not limited to the above embodiments, and any changes or substitutions that can be easily understood by those skilled in the art within the technical scope of the present invention are intended to be included in the scope of the present invention. Therefore, the protection scope of the invention is subject to the protection scope of the claims.
Claims (10)
1. A microscopy apparatus, comprising:
a fluorescence field illumination assembly (110) configured to excite fluorescence of a sample (300);
a bright field illumination assembly (130) configured to illuminate the sample (300) to provide a bright field to the sample (300);
an image acquisition component (150) configured to acquire an image of a sample (300) under illumination by the fluorescent field illumination component (110) and/or the bright field illumination component (130); the method comprises the steps of,
a dichroic mirror group (170) configured to transmit a light beam emitted by the fluorescent field illumination assembly (110) and/or the bright field illumination assembly (130) to the sample (300) and to transmit fluorescence and/or reflected light excited by the sample (300) to the image acquisition assembly (150).
2. The microscopy apparatus of claim 1, wherein the dichroic mirror set (170) comprises a first dichroic mirror (173) and a second dichroic mirror (175);
wherein the fluorescent field illumination assembly (110) and the bright field illumination assembly (130) are respectively arranged on a first incident side and a second incident side of the first dichroic mirror (173), and the first dichroic mirror (173) can make light rays incident on the first incident side and the second incident side emitted from a first emergent side;
the second dichroic mirror (175) is disposed on a first outgoing side of the first dichroic mirror (173), and a light beam emitted through the first outgoing side can be projected to a sample (300) through the second dichroic mirror (175);
the second dichroic mirror (175) may also project reflected light and/or excited fluorescence light of the sample (300) to the image acquisition assembly (150).
3. The microscopy device according to claim 2, wherein the first dichroic mirror (173) is adapted to refract the light beam incident in the first direction of incidence such that it passes through the first dichroic mirror (173) and exits from the first direction of exit of the first dichroic mirror (173);
the first dichroic mirror (173) may reflect the light beam incident in the second incident direction such that the light beam is emitted from the first emitting direction of the first dichroic mirror (173) after passing through the first dichroic mirror (173).
4. A microscopy apparatus according to claim 3, characterized in that the second dichroic mirror (175) is parallel to the first dichroic mirror (173);
the light beam emitted by the first emergent direction of the first dichroic mirror (173) can be reflected by the second dichroic mirror (175) and can be projected to a sample (300);
reflected light and/or excited fluorescence of the sample (300) is refracted by the second dichroic mirror (175) and is transmitted through the second dichroic mirror (175) to the image acquisition assembly (150).
5. The microscopy device of any of claims 2-4, further comprising a sample holder (190);
the sample rack (190) is arranged at one side of the second dichroic mirror, and the sample rack (190) is used for carrying a sample (300);
-the sample holder (190), the second dichroic mirror (175) and the image acquisition assembly (150) are located on a first axis;
-the fluorescent field illumination assembly (110), the first dichroic mirror (173) and the second dichroic mirror (175) are located on a second axis;
the first axis and the second axis are perpendicular, and the second dichroic mirror (175) has an angle of 45 ° with respect to both the first axis and the second axis.
6. The microscopy apparatus according to any of claims 2-4, wherein the fluorescent field illumination assembly (110) comprises an excitation light source (111) and a first filter (113);
the excitation light source (111) is disposed on a first incident side of the first dichroic mirror (173), and the first filter (113) is disposed between the first dichroic mirror (173) and the excitation light source (111).
7. The microscopy apparatus according to any of claims 2-4, wherein the bright field illumination assembly (130) comprises a light source (131) and a second filter (133);
the light source (131) is disposed on a second incident side of the first dichroic mirror (173), and the second filter (133) is disposed between the first dichroic mirror (173) and the light source (131).
8. The microscopy apparatus of any of claims 2-4, wherein the image acquisition assembly (150) comprises a camera (155), a corrective lens (153), and a third filter (151);
the camera (155), the correction lens (153) and the third filter (151) are sequentially disposed on one side of the second dichroic mirror (175).
9. The microscopy apparatus of claim 5, further comprising a lens group (180) for adjusting magnification;
the lens group (180) is disposed between the sample holder (190) and the second dichroic mirror (175).
10. The microscopy device of any of claims 2-4, further comprising a collection mirror;
the condenser is provided between the first dichroic mirror (173) and the second dichroic mirror (175).
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| CN202311641119.2A CN117348224A (en) | 2023-12-04 | 2023-12-04 | Microscopic device |
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