CN117338471A - Three-dimensional quantitative injection instrument for vertebral bodies of mouse spines - Google Patents
Three-dimensional quantitative injection instrument for vertebral bodies of mouse spines Download PDFInfo
- Publication number
- CN117338471A CN117338471A CN202311556860.9A CN202311556860A CN117338471A CN 117338471 A CN117338471 A CN 117338471A CN 202311556860 A CN202311556860 A CN 202311556860A CN 117338471 A CN117338471 A CN 117338471A
- Authority
- CN
- China
- Prior art keywords
- sliding rail
- dimensional
- module
- axis
- axis sliding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002347 injection Methods 0.000 title claims abstract description 23
- 239000007924 injection Substances 0.000 title claims abstract description 23
- 238000000520 microinjection Methods 0.000 claims abstract description 17
- 230000007246 mechanism Effects 0.000 claims abstract description 14
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 13
- 230000036760 body temperature Effects 0.000 claims description 13
- 238000010438 heat treatment Methods 0.000 claims description 13
- 241000699670 Mus sp. Species 0.000 claims description 12
- 239000003994 anesthetic gas Substances 0.000 claims description 11
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 11
- 238000012423 maintenance Methods 0.000 claims description 9
- 238000009423 ventilation Methods 0.000 claims description 8
- 230000000630 rising effect Effects 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 5
- 230000009467 reduction Effects 0.000 claims description 3
- 210000004556 brain Anatomy 0.000 abstract description 11
- 210000000988 bone and bone Anatomy 0.000 abstract description 10
- 238000011160 research Methods 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 4
- 239000000700 radioactive tracer Substances 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 2
- 206010002091 Anaesthesia Diseases 0.000 description 11
- 230000037005 anaesthesia Effects 0.000 description 11
- 208000001132 Osteoporosis Diseases 0.000 description 8
- 238000010586 diagram Methods 0.000 description 6
- 210000001015 abdomen Anatomy 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- POIUWJQBRNEFGX-XAMSXPGMSA-N cathelicidin Chemical compound C([C@@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(C)C)C1=CC=CC=C1 POIUWJQBRNEFGX-XAMSXPGMSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 208000010392 Bone Fractures Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000002631 hypothermal effect Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 210000003815 abdominal wall Anatomy 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003444 anaesthetic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000000399 orthopedic effect Effects 0.000 description 1
- 230000011164 ossification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 210000004303 peritoneum Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
- A61D7/04—Devices for anaesthetising animals by gases or vapours; Inhaling devices
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
- A61D2003/006—Appliances for supporting or fettering animals for operative purposes with leg or foot restraining means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Animal Husbandry (AREA)
- Anesthesiology (AREA)
- Surgery (AREA)
- Instructional Devices (AREA)
Abstract
The invention discloses a three-dimensional quantitative injection instrument in a vertebral body of a mouse, which comprises a platform, a three-dimensional micro injection module, a traction module and a fixing module, wherein the three-dimensional micro injection module is arranged on the platform and comprises a three-dimensional moving mechanism and an injector, the injector is arranged at the terminal of the three-dimensional moving mechanism, and the injector can be suspended above an operation area; the traction module comprises a traction slide rail, a spring drag hook is arranged on the traction slide rail in a sliding manner, and a locking knob is arranged on the traction slide rail; the fixing module comprises a fixing ring supporting column, a knob fixing cap and four limb fixing rings are arranged on the fixing ring supporting column, the other end of the tensioning rope is connected with the four limb fixing rings, and one end of the tensioning rope is wound around the knob fixing cap; the problem that no suitable instrument is available for bone positioning injection in brain bone crossing research is solved as a whole; the three-dimensional microinjection module changes the problem of hand shake of the original freehand in the process of injecting the medicine or the tracer into the vertebral column body for a long time.
Description
Technical Field
The invention belongs to the field of interdisciplinary research devices of neuroscience and orthopedics, and particularly relates to a research device for three-dimensional quantitative injection in a vertebral body of a mouse.
Background
Today, with ever-increasing life expectancy and increasing aging population, a silent disease afflicts an increasing number of people, namely osteoporosis. Osteoporosis, which is known as diabetes and cardiovascular disease, is called a "silent killer" that limits the health and quality of life of the middle-aged and elderly. Clinical study data showed that the incidence of osteoporosis was nearly 30% in female populations and nearly 20% in male populations. Osteoporosis has no obvious clinical symptoms in early stage and is often ignored by people. However, high mortality and high disability rates are hidden behind them, which in turn leads to a reduction in quality of life and an increase in economic burden. Therefore, early discovery, early diagnosis and early treatment are also applicable to the prevention and treatment of osteoporosis.
At present, the osteoporosis treatment method widely applied clinically is concentrated on bones, and mainly comprises two ideas: inhibit bone fracture and promote bone formation. However, these therapies all have varying degrees of side effects. In order to better serve the vast osteoporosis population, a new prevention and treatment thought needs to be explored.
The brain is the "commander" of the human body, which controls one action of the human body. With the proposal of brain planning in various countries, the steps for exploring the most mystery organ of the human body are faster and faster. From clinical studies to basic exploration, more and more scholars gradually aim at reducing the connection between the brain and peripheral organs. Such as: the fracture recovery time of the depressed patients is longer than that of normal people, the risk of osteoporosis of the people suffering from long-term satiety stress is higher than that of normal people, and the research results are pointed to a point that the brain and the bone are indistinguishable.
Clinical studies reveal phenomena, basic studies seek true theory. However, it has been almost full of difficulty in the past to put together the two seemingly uninfluenced organs of the "brain" -the most flexible organs of the human body and the "bones" -the most rigid organs of the human body. With the rapid development of brain science, a technological breakthrough has made these difficulties progressively overcome. However, a practical difficulty presented to researchers is that instruments are not well suited. In order to better advance brain-bone crossing research, a three-dimensional quantitative injection device in the vertebral body of the spine is designed aiming at the practical needs in the research.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a three-dimensional quantitative injection instrument for the vertebral bodies of the spines of mice, which comprises a three-dimensional micro injection module, a traction module, a fixing module, an anesthesia and respiration support module, a body temperature maintenance module and a cold light illumination module, is 'multi-position integrated', solves the problem that the existing instrument is not applicable, overcomes the problems of stable needle points of the original manual exposure operation area, stable freehand maintenance injection, low body temperature in the perioperative period, incomplete anesthesia and the like one by one, ensures the injection precision and accuracy, improves the living quality in the perioperative period, and effectively improves the experimental success rate.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: the three-dimensional quantitative injection instrument comprises a platform, a three-dimensional microinjection module, a traction module and a fixing module, wherein the three-dimensional microinjection module, the traction module and the fixing module are arranged on the platform, the three-dimensional microinjection module comprises a three-dimensional moving mechanism and an injector, the injector is arranged at the terminal of the three-dimensional moving mechanism, and the injector can be suspended above an operation area; the traction module comprises a traction slide rail, a spring drag hook is arranged on the traction slide rail in a sliding manner, and a locking knob is arranged on the traction slide rail; the fixing module comprises a fixing ring supporting column, a knob fixing cap and four limbs fixing rings are arranged on the fixing ring supporting column, the other end of the tensioning rope is connected with the four limbs fixing rings, and one end of the tensioning rope is wound on the knob fixing cap.
Further, the knob fixing cap is connected with the fixing ring support column by a ratchet structure.
Further, the traction slide rail is cylindrical, a rigid connecting rod is arranged on the spring drag hook in a sliding mode on the traction slide rail, one end of the rigid connecting rod is sleeved on the traction slide rail, a retracting screw is arranged at the joint of the traction slide rail in a sleeved mode, and the other end of the rigid connecting rod is connected with the spring drag hook.
Further, the three-dimensional moving mechanism comprises a Y-axis sliding rail, a Z-axis sliding rail and an X-axis sliding rail, the Y-axis sliding rail, the Z-axis sliding rail and the X-axis sliding rail all adopt a screw rod structure, the Z-axis sliding rail is connected with the moving part of the X-axis sliding rail, the Y-axis sliding rail is connected with the moving part of the Z-axis sliding rail, the moving part of the Y-axis sliding rail is used as a terminal of the three-dimensional moving mechanism, the injector is arranged on the moving part of the Y-axis sliding rail, the holder is arranged on the moving part of the Y-axis sliding rail, and the injector is arranged in the holder.
Further, Y axle slide rail, Z axle slide rail and X axle slide rail correspond respectively and set up Y axle knob, Z axle knob and X axle knob, all set up the scale on Y axle slide rail, Z axle slide rail and the X axle slide rail to set up the indication mark on the Z axle knob.
Further, the Z-axis sliding rail is rotationally connected with the X-axis sliding rail, and a Z-axis rotary dial is arranged at the joint of the Z-axis sliding rail and the X-axis sliding rail.
Further, the device is also provided with a body temperature maintaining module, wherein the body temperature maintaining module comprises a temperature control switch, a temperature reducing button, a temperature rising button and a heating pad, the heating pad is connected with the temperature control switch, and the temperature control switch is connected with the temperature reducing button and the temperature rising button; the heating pad is laid at the bottom of the operation area.
Further, a cold light source machine is arranged on the platform, a lighting switch is arranged on the cold light source machine, the cold light source machine is connected with a light source lamp tube, the light source lamp tube is a universal tube, and the lamp holder can be close to an operation area.
Furthermore, an anesthetic gas generating device is arranged on the platform and connected with the breathing mask through a ventilation catheter, the ventilation catheter is a universal tube, and a head baffle is arranged in the head area of the mouse.
Compared with the prior art, the invention has at least the following beneficial effects: the problem that no suitable instrument is available for bone positioning injection in brain bone crossing research is solved as a whole; the three-dimensional micro injection module changes the problem of hand shake of the original freehand in the process of injecting the medicine or the tracer into the vertebral body of the spine for a long time, ensures that the needle is accurately inserted into the vertebral body, and stably injects the research reagent into the vertebral body; the traction module realizes the possibility of independent operation of the anterior spinal operation on small animals, ensures that the operation area is fully exposed and does not influence the intraspinal injection operation; the fixing module slightly pulls and fixes the limbs of the mice, so that the whole operation area is ensured to be stable and not to shake, and the normal operation of the operation is effectively ensured;
furthermore, the anesthesia and respiration support module is integrated on the stereotactic instrument, so that the traditional framework that anesthesia and respiration support is required to be additionally configured in brain stereotactic operation is changed, and the operation and the use of experimental staff are facilitated.
Further, the body temperature maintenance module integrates a commercially available heating pad for animals on the positioning instrument, monitors and maintains the body temperature in real time, ensures that the poor accident of hypothermia does not occur in the perioperative period, powerfully ensures the perioperative period safety of mice, lays a good foundation for the development of subsequent experiments, ensures that the operation area is bright, and ensures that the operation is normally performed.
Drawings
FIG. 1 is a schematic view of the present invention;
FIG. 2 is a schematic diagram of the front view structure of the three-dimensional microinjection module of the present invention;
FIG. 3 is a schematic top view of the three-dimensional microinjection module of the present invention;
fig. 4 is a right-view structural schematic diagram of the three-dimensional stereoscopic microinjection module of the present invention;
FIG. 5 is a schematic diagram of the front view of the pulling module of the present invention;
FIG. 6 is a schematic top view of the pulling module of the present invention;
fig. 7 is a right-side view of the drawing module of the present invention;
FIG. 8 is a schematic view of a fastening module according to the present invention;
FIG. 9 is a schematic diagram showing the front view of the anesthesia and breathing support module of the present invention;
FIG. 10 is a schematic top view of the anesthesia and ventilation support module of the present invention;
FIG. 11 is a schematic diagram of a right-side view of the anesthesia and breathing support module of the present invention;
fig. 12 is a schematic top view of the body temperature maintenance module according to the present invention;
FIG. 13 is a schematic diagram of the front view of the lighting module of the present invention;
FIG. 14 is a schematic top view of a lighting module according to the present invention;
fig. 15 is a right-view schematic structure of the lighting module of the present invention.
In the drawings, microinjection module: 1-clamp holder, 2-Z axis knob, 3-Y axis knob, 4-Y axis slide rail, 5-Z axis slide rail, 6-Z axis rotary dial, 7-sliding device, 8-positioning slide rail support column, 9-X axis slide rail, 10-X axis knob; body temperature maintenance module: 11-temperature display screen, 12-cooling button, 13-temperature control switch, 14-heating button, 15-heating pad. And (3) fixing a module: 16-limb fixing rings, 17-fixing ring supporting columns and 18-knob fixing caps; and (3) a traction module: 19-pulling slide rails, 20-spring drag hooks, 21-pulling slide devices, 22-pulling slide rail support columns and 23-locking knobs; anesthesia and respiration support module: 24-breathing mask, 25-anesthetic gas generating device, 26-ventilation catheter, 27-anesthetic gas switch; an illumination module: 28-a cold light source machine, 29-a light source lamp tube and 30-a lighting switch; 31-metal bottom plate.
Detailed Description
The following specific embodiments of the present invention are provided, and it should be noted that the present invention is not limited to the following specific embodiments, and all equivalent changes made on the basis of the technical solutions of the present application fall within the protection scope of the present invention.
Referring to fig. 1, a three-dimensional quantitative injection instrument for a vertebral body of a mouse comprises a platform, and a three-dimensional microinjection module, a traction module and a fixing module which are arranged on the platform, wherein the three-dimensional microinjection module comprises a three-dimensional moving mechanism and an injector, the injector is arranged at the terminal of the three-dimensional moving mechanism, and the injector can be suspended above an operation area; the traction module comprises a traction slide rail 19, a spring drag hook 20 is arranged on the traction slide rail 19 in a sliding manner, and a locking knob 23 is arranged on the traction slide rail 19; the fixing module comprises a fixing ring supporting column 17, a knob fixing cap 18 and an extremity fixing ring 16 are arranged on the fixing ring supporting column 17, the other end of the tensioning rope is connected with the extremity fixing ring 16, and one end of the tensioning rope is wound around the knob fixing cap 18.
As a preferred embodiment, knob retaining cap 18 is connected to retaining ring support post 17 using a ratchet arrangement.
Referring to fig. 5, 6, 7 and 8, optionally, the pulling slide rail 19 is cylindrical, a rigid connecting rod is arranged on the spring drag hook 20 and is slidably arranged on the pulling slide rail 19, one end of the rigid connecting rod is sleeved on the connecting part of the pulling slide rail 19 and is provided with a retraction screw, and the other end of the rigid connecting rod is connected with the spring drag hook 20, so that the pulling angle can be changed, and the requirements of different situations can be met.
Referring to fig. 1, 2, 3 and 4, the three-dimensional moving mechanism comprises a Y-axis sliding rail 4, a Z-axis sliding rail 5 and an X-axis sliding rail 9, wherein the Y-axis sliding rail 4, the Z-axis sliding rail 5 and the X-axis sliding rail 9 all adopt screw rod structures, the Z-axis sliding rail 5 is connected with the moving part of the X-axis sliding rail 9, the Y-axis sliding rail 4 is connected with the moving part of the Z-axis sliding rail 5, the moving part of the Y-axis sliding rail 4 serves as a terminal of the three-dimensional moving mechanism, an injector is mounted on the moving part of the Y-axis sliding rail 4, a holder 1 is mounted on the moving part of the Y-axis sliding rail 4, and the injector is mounted in the holder 1; the Z-axis sliding rail 5 is rotationally connected with the X-axis sliding rail 9, the output part of the X-axis sliding rail 9 is provided with a connecting pipe sleeve which is arranged on the outer side of the X-axis sliding rail 9, the Z-axis sliding rail 5 is of a pipe shape, a locking screw is arranged between the connecting pipe and the outer surface of the X-axis sliding rail 9, after the Z-axis sliding rail 5 is rotated, locking is carried out, the X-axis sliding rail 9 is provided with a Z-axis rotating dial 6, and the rotating angle of the Z-axis sliding rail 5 relative to the X-axis sliding rail 9 can be observed at any time.
The Y-axis sliding rail 4, the Z-axis sliding rail 5 and the X-axis sliding rail 9 are respectively correspondingly provided with a Y-axis knob 3, a Z-axis knob 2 and an X-axis knob 10, scales are respectively arranged on the Y-axis sliding rail 4, the Z-axis sliding rail 5 and the X-axis sliding rail 9, and indication marks are arranged on the Z-axis knob 2, so that the moving distance and the rotating angle can be accurately acquired.
Another embodiment can also be adopted, the Z-axis sliding rail 5 is rotationally connected with the X-axis sliding rail 9, and the Z-axis rotary dial 6 is arranged at the joint of the Z-axis sliding rail 5 and the X-axis sliding rail 9.
Referring to fig. 1 and 12, a body temperature maintaining module is further provided, the body temperature maintaining module comprises a temperature control switch 13, a temperature reducing button 12, a temperature rising button 14 and a heating pad 15, the heating pad 15 is connected with the temperature control switch 13, and the temperature control switch 13 is connected with the temperature reducing button 12 and the temperature rising button 14; the heating pad 15 is laid at the bottom of the operation region.
Referring to fig. 7, a traction slide support column 22 is linked below the traction slide 19, a traction slide 21 is sleeved outside the traction slide 19, a screwing knob 23 is arranged on the traction slide 21, the traction slide 21 is manually slid, and the position of the traction slide 21 on the traction slide 19 is locked by the screwing knob 23.
Referring to fig. 1, 13 and 14, in order to provide a light environment convenient to operate, a cold light source machine 28 is further arranged on the platform, a lighting switch 30 is arranged on the cold light source machine 28, the cold light source machine 28 is connected with a light source tube 29, the light source tube 29 is a universal tube, and the lamp cap can be close to an operation area.
Referring to fig. 1, 9, 10, 11 and 15, an anesthetic gas generating device 25 is disposed on the platform, the anesthetic gas generating device 25 is connected with a breathing mask 24 through a ventilation catheter 26, the ventilation catheter is a universal tube, and a head baffle is disposed in the head area of the mouse, so that an anesthetic function can be improved, operators can be reduced, and operation efficiency is improved.
Before the experiment was started, it was checked whether each module was operating properly. After the examination is finished, the four-limb fixing ring 16 of the fixing module is used for placing the mouse after induced anesthesia on the heating pad 15 of the body temperature maintaining module, one end of the four-limb fixing ring 16 is sleeved on the fixing ring supporting column 17, and the other end of the four-limb fixing ring is sleeved on the four limbs of the mouse, so that the knob fixing cap 18 is locked after the four limbs are in a proper traction state. The mouse mouth and nose are placed in the breathing mask 24, an anesthetic gas switch 27 on the surface of an anesthetic gas generating device 25 of the anesthesia and breathing support module is turned on, and anesthetic gas is delivered to the mouth and nose of the mouse through an airway 26, so that the anesthesia state in operation is maintained. Clicking the illumination switch 29 on the cold light source machine 28 aligns the light source tube 29 to the mouse abdomen.
After the preparation, the abdomen of the mouse is prepared into skin and sterilized, and then the abdomen is opened layer by layer. The abdominal wall is pulled by the spring retractor 20 of the pulling module, and the pulling slide 21 is adjusted along the pulling slide 19 so that the operating field is fully exposed. After being adjusted to a proper position, the locking knob 23 is screwed down, so that the abdomen is in a fixed exposure state, and the subsequent operation is convenient.
The abdominal intestine was gently pulled out and covered with warm saline gauze to keep moist. The posterior peritoneum was exposed and cut to fully expose the anterior lumbar vertebral body.
The microinjector is fixed on the clamp holder 1, a proper amount of tracer is sucked, the sliding rail 9, the sliding rail 5 and the sliding rail 4 slowly move through the X-axis knob 10, the Y-axis knob 3 and the Z-axis knob 2, the needle point of the microinjector is moved to the surface of the vertebral body, the needle is slowly inserted into the vertebral body through fine adjustment of the Z-axis knob 2, the tracer is injected at a stable speed, the needle is left for 15min after the injection is finished, and the Z-axis button 2 is slowly rotated out. The needle tract is plugged by bone wax.
After slowly removing the injector, the intestinal canal is also retracted, the abdomen traction is released, and the abdominal cavity is closed layer by layer.
The temperature display screen 11 of the temperature detection module is used for displaying the temperature in real time in the operation, the temperature control switch 13 is turned on as required, and the output temperature of the temperature maintenance module is changed through the temperature raising key 14 or the temperature lowering key 12, so that the phenomenon of hypothermia in the operation of the mice is avoided.
The anesthetic gas switch 27 and the illumination switch 30 are turned off after the operation to clean and sterilize the instrument.
The invention has the advantages of solving the difficult situation that the instrument is not applicable in the brain bone crossing research, integrating all elements in the three-dimensional positioning process, solving the first step in the brain bone crossing research process, namely accurate positioning and accurate injection, being convenient for operation and life maintenance in the perioperative period, and improving the success rate of experiments.
Claims (9)
1. The three-dimensional quantitative injection instrument for the vertebral bodies of the mouse is characterized by comprising a platform, a three-dimensional microinjection module, a traction module and a fixing module, wherein the three-dimensional microinjection module, the traction module and the fixing module are arranged on the platform, the three-dimensional microinjection module comprises a three-dimensional moving mechanism and an injector, the injector is arranged at the terminal of the three-dimensional moving mechanism, and the injector can be suspended above an operation area; the traction module comprises a traction slide rail (19), a spring drag hook (20) is arranged on the traction slide rail (19) in a sliding manner, and a locking knob (23) is arranged on the traction slide rail (19); the fixing module comprises a fixing ring supporting column (17), a knob fixing cap (18) and four limb fixing rings (16) are arranged on the fixing ring supporting column (17), the other end of the tensioning rope is connected with the four limb fixing rings (16), and one end of the tensioning rope is wound on the knob fixing cap (18).
2. The three-dimensional quantitative injection instrument for the vertebral bodies of the spines of mice according to claim 1, wherein a knob fixing cap (18) is connected with a fixing ring supporting column (17) by adopting a ratchet structure.
3. The three-dimensional quantitative injection instrument for the vertebral bodies of the spines of mice according to claim 1, wherein the traction slide rail (19) is cylindrical, a spring drag hook (20) is arranged on the traction slide rail (19) in a sliding manner, one end of the rigid link is sleeved on the traction slide rail (19), one end of the rigid link is sleeved on a joint of the traction slide rail (19) and is provided with a retracting screw, and the other end of the rigid link is connected with the spring drag hook (20).
4. The three-dimensional quantitative injector in a vertebral body of a mouse spinal column according to claim 1, wherein the three-dimensional moving mechanism comprises a Y-axis sliding rail (4), a Z-axis sliding rail (5) and an X-axis sliding rail (9), the Y-axis sliding rail (4), the Z-axis sliding rail (5) and the X-axis sliding rail (9) are all of screw rod structures, the Z-axis sliding rail (5) is connected with a moving part of the X-axis sliding rail (9), the Y-axis sliding rail (4) is connected with a moving part of the Z-axis sliding rail (5), the moving part of the Y-axis sliding rail (4) serves as a terminal of the three-dimensional moving mechanism, the injector is mounted on the moving part of the Y-axis sliding rail (4), the gripper (1) is mounted on the moving part of the Y-axis sliding rail (4), and the injector is mounted in the gripper (1).
5. The three-dimensional quantitative injector for the vertebral bodies of the spines of mice according to claim 4, wherein a Y-axis knob (3), a Z-axis knob (2) and an X-axis knob (10) are respectively and correspondingly arranged on a Y-axis slide rail (4), a Z-axis slide rail (5) and an X-axis slide rail (9), scales are respectively arranged on the Y-axis slide rail (4), the Z-axis slide rail (5) and the X-axis slide rail (9), and indication marks are arranged on the Z-axis knob (2).
6. The three-dimensional quantitative injection instrument for the vertebral bodies of the spines of mice according to claim 4, wherein the Z-axis sliding rail (5) is rotationally connected with the X-axis sliding rail (9), and a Z-axis rotary dial (6) is arranged at the joint of the Z-axis sliding rail (5) and the X-axis sliding rail (9).
7. The three-dimensional quantitative injection instrument for the vertebral bodies of the spines of the mice according to claim 1, wherein the three-dimensional quantitative injection instrument is further provided with a body temperature maintenance module, the body temperature maintenance module comprises a temperature control switch (13), a temperature reduction button (12), a temperature rising button (14) and a heating pad (15), the heating pad (15) is connected with the temperature control switch (13), and the temperature control switch (13) is connected with the temperature reduction button (12) and the temperature rising button (14); the heating pad (15) is laid at the bottom of the operation area.
8. The three-dimensional quantitative injection instrument for the vertebral bodies of the spines of mice according to claim 1, wherein a cold light source machine (28) is further arranged on the platform, an illumination switch (30) is arranged on the cold light source machine (28), the cold light source machine (28) is connected with a light source lamp tube (29), the light source lamp tube (29) is a universal tube, and the lamp cap can be close to an operation area.
9. The three-dimensional quantitative injection instrument for the vertebral bodies of the spines of mice according to claim 1, wherein an anesthetic gas generating device (25) is arranged on the platform, the anesthetic gas generating device (25) is connected with a breathing mask (24) through a ventilation catheter (26), the ventilation catheter is a universal tube, and a head baffle is arranged in the head area of the mice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311556860.9A CN117338471A (en) | 2023-11-21 | 2023-11-21 | Three-dimensional quantitative injection instrument for vertebral bodies of mouse spines |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311556860.9A CN117338471A (en) | 2023-11-21 | 2023-11-21 | Three-dimensional quantitative injection instrument for vertebral bodies of mouse spines |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117338471A true CN117338471A (en) | 2024-01-05 |
Family
ID=89355899
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311556860.9A Pending CN117338471A (en) | 2023-11-21 | 2023-11-21 | Three-dimensional quantitative injection instrument for vertebral bodies of mouse spines |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117338471A (en) |
-
2023
- 2023-11-21 CN CN202311556860.9A patent/CN117338471A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109567923A (en) | A kind of personalization Pedicle Screw Placement in Lower Cervical navigation template device and preparation method thereof step by step | |
| CN203861509U (en) | General nursing instrument | |
| CN201675917U (en) | Novel video laryngoscope | |
| CN117338471A (en) | Three-dimensional quantitative injection instrument for vertebral bodies of mouse spines | |
| CN208823264U (en) | The postoperative leg fixer of neurosurgical patients | |
| CN204091929U (en) | The built-in expansion testing fixture of oral and maxillofacial surgery | |
| CN205924087U (en) | Drag hook support for thyroid surgery | |
| CN215740235U (en) | Orthopedic surgery positioning device | |
| CN211583204U (en) | Disposable sterile vaginal marker | |
| CN109350197A (en) | The accurate Needle localization support system of intracranial hematoma Minimally invasive puncture | |
| CN209808621U (en) | Operating table for experiment small animal head and neck position operation | |
| CN208926734U (en) | Children's femur adjustable lever towing bracket | |
| CN112370094A (en) | Adjustable vagina expanding instrument for gynecological operation | |
| Wheeless Jr | An inexpensive laparoscopy system for female sterilization | |
| CN208598451U (en) | Combined type neurosurgery retractor | |
| CN210810916U (en) | Hand-operated tongue pressing device for pediatric oral cavity | |
| CN102499753B (en) | Adjustable direct laryngoscope | |
| CN213249601U (en) | Pre-tightening type laser positioner for skull traction head ring | |
| CN2698277Y (en) | Auxiliary device for intervertebral disk scope working channel used in upper cervical vertebra anterior surgery | |
| BR102019022396A2 (en) | channel stretch device, device and kit production process | |
| Zajac | Speech aerodynamics of cleft palate | |
| CN213723230U (en) | Special bed of acupuncture treatment | |
| CN211610578U (en) | Arm suspension device for operation | |
| CN203687933U (en) | Nursing body position angle tester | |
| CN210472292U (en) | Cosmetic surgery mandible plastic assistor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |