CN117305165A - Microbial composite microbial agent and method for treating excess sludge - Google Patents

Microbial composite microbial agent and method for treating excess sludge Download PDF

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Publication number
CN117305165A
CN117305165A CN202311259007.0A CN202311259007A CN117305165A CN 117305165 A CN117305165 A CN 117305165A CN 202311259007 A CN202311259007 A CN 202311259007A CN 117305165 A CN117305165 A CN 117305165A
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sludge
microbial
fermented
treatment
microbial composite
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杨朝伟
谢建平
张海鹰
徐凡钦
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Guangdong Dingtai Electric Appliance Co ltd
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Guangdong Dingtai Electric Appliance Co ltd
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Abstract

The invention provides a microbial composite microbial agent and application thereof in treating excess sludge, and belongs to the technical field of excess sludge treatment, wherein the microbial composite microbial agent comprises bacillus subtilis, alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, bacillus amyloliquefaciens and actinomyces; the enzyme preparation comprises protease, cellulase, amylase and lysozyme; the method of the invention will comprise the steps of: mixing the dehydrated residual sludge with a surfactant; mixing the treated material with a microbial composite microbial agent for fermentation treatment; and performing ultrasonic treatment again, mixing with the enzyme preparation, performing enzymolysis and solid-liquid separation, and drying the solid. The microbial composite microbial agent has reasonable proportion, and all the strains are matched with each other to play a role in synergy; the method for treating the sludge by the microbial composite microbial agent provided by the invention is used for treating the residual sludge, and has the advantages of simplicity in operation, short period, high sludge reduction ratio and no secondary pollution.

Description

Microbial composite microbial agent and method for treating excess sludge
Technical Field
The invention belongs to the technical field of excess sludge treatment, and particularly relates to a microbial composite bacterial agent and a method for treating excess sludge.
Background
With the increasing of sewage treatment capacity, the discharge amount of residual sludge which is a by-product of sewage treatment is also increasing, and the residual sludge has complex components and contains sediment, colloid, organic matters, microorganisms, metal elements and the like. The organic matters in the biological sludge are high in concentration and difficult to degrade, contain more pathogen microorganisms, and can cause secondary pollution to the environment, such as water and soil if the biological sludge is improperly treated.
The main treatment method of the residual sludge at present comprises the following steps: the technology adopts earthworms to treat residual sludge, such as ' construction of an earthworm biofilter and inoculation of earthworms ' is disclosed in China patent CN 101348315B, 10-13 mm and 6-9 mm ceramsite are filled in the upper layer and the lower layer in the filter, simultaneously, eisenia foetida is uniformly inoculated in the ceramsite according to 1.13 multiplied by 105/m 3 filter materials with volume density, holes with uniform distribution are formed on the bottom plate of the filter, sludge flows through earthworms among ceramic grains which are successfully domesticated from a water distributor at the top end of the filter, sludge organic matters are decomposed into nutrients of the earthworms and other microorganisms under the action of enzyme systems such as protease and the like which are abundant by the earthworms, the sludge organic matters flow into a sedimentation tank from the holes at the bottom of the filter tank, and a soil modifier with strong fertilizer efficiency which is coexistent with earthworm manure is collected and dehydrated ' is disclosed in China patent CN 107827332A ' the method for treating residual sludge by anaerobic fermentation reaction coupling microbial electrolysis ' is also disclosed in the technology, and the method is characterized in that the residual sludge is fully produced by coupling MEC with an anaerobic digestion reactor, the sludge is not only degraded by the organic matters, and the residual gas is fully degraded by the organic matters in the filter tank, and the residual gas is fully produced by the method.
However, the existing treatment method of the excess sludge still has the problems of complex operation, long period and poor treatment effect.
Disclosure of Invention
In view of the above, the invention aims to provide a microbial composite microbial agent and a method for treating excess sludge, wherein the microbial composite microbial agent has reasonable proportion, and all strains are mutually matched to act cooperatively; the method for treating the sludge by the microbial composite microbial agent provided by the invention is used for treating the residual sludge, and has the advantages of simplicity in operation, short period, high degradation rate and no secondary pollution.
The invention provides a microbial composite microbial agent for treating excess sludge, which comprises bacillus subtilis, alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, bacillus amyloliquefaciens and actinomycetes; the mass ratio of the bacillus subtilis to the alcaligenes faecalis to the aspergillus niger to the bacillus stearothermophilus to the bacillus amyloliquefaciens to the actinomycetes is (5-11): (2-4): (2-4): (1-3): (6-9): (5-8).
Preferably, the total viable count of the microbial composite microbial agent is more than or equal to 5 multiplied by 10 9 /g。
The invention provides an enzyme preparation for treating excess sludge, which comprises protease, cellulase, amylase and lysozyme;
the mass ratio of the protease, the cellulase, the amylase and the lysozyme is (4-6): (2-4): (2-4): (6-8).
The invention provides a method for treating excess sludge, which comprises the following steps:
1) Dehydrating the residual sludge until the water content is 70-80%, and mixing the residual sludge with a surfactant to obtain pretreated sludge;
2) Ultrasonic treatment is carried out on the pretreated sludge to obtain sludge to be fermented;
3) Mixing the microbial composite bacterial agent with the sludge to be fermented to perform fermentation treatment to obtain fermented sludge;
4) Treating the fermented sludge by ultrasonic waves, and then mixing the fermented sludge with the enzyme preparation for enzymolysis to obtain the sludge after enzymolysis;
5) And (3) carrying out solid-liquid separation on the sludge after enzymolysis, and drying solid matters.
Preferably, the surfactant in the step 1) is rhamnolipid, and the mass ratio of the surfactant to the residual sludge is 1: (100-300).
Preferably, the intensity of the ultrasonic treatment in the step 2) is 0.5-1.0W/ml, and the time of the ultrasonic treatment is 10-15 min.
Preferably, the mass ratio of the microbial composite microbial agent to the sludge to be fermented in the step 3) is (1-5): 100.
preferably, the intensity of the ultrasonic treatment in the step 4) is 0.5-1.0W/ml, and the time of the ultrasonic treatment is 5-10 min; the addition amount of the enzyme preparation is 3-5 wt% of the fermented sludge.
Preferably, the enzymolysis time is 3-5 hours.
Compared with the prior art, the invention has the following beneficial effects: the microbial composite microbial agent and the method for treating the excess sludge are simple to operate, can rapidly degrade organic matters in the excess sludge, are safe and efficient, and do not produce secondary pollution. The solubilization can be realized by pretreating the residual sludge with the surfactant; the ultrasonic treatment of the surplus sludge can break up the floc structure of the sludge, which is beneficial to the dispersion of the sludge, and the lysis of the ultrasonic wave can kill harmful bacteria in the sludge, which is beneficial to the cracking of the original microorganism in the sludge and the outflow of intracellular substances, thereby providing more favorable conditions for the subsequent fermentation of the microorganism composite microbial inoculum; further combines the fermentation of the compound microorganism and the enzymolysis of the enzyme preparation, can thoroughly reduce the organic matters in the sludge and realize harmless treatment.
Detailed Description
The invention provides a microbial composite microbial agent for treating excess sludge, which comprises bacillus subtilis, alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, bacillus amyloliquefaciens and actinomycetes; the mass ratio of the bacillus subtilis to the alcaligenes faecalis to the aspergillus niger to the bacillus stearothermophilus to the bacillus amyloliquefaciens to the actinomycetes is (5-11): (2-4): (2-4): (1-3): (6-9): (5-8).
In the invention, the mass ratio of the bacillus subtilis, the alcaligenes faecalis, the aspergillus niger, the bacillus stearothermophilus, the bacillus amyloliquefaciens and the actinomycetes is preferably (6-10): (2.5-3.5): (2.5-3.5): (1.5-2.5): (7-8): (6 to 7), more preferably (7 to 9): 3:3:2:7.5:6.5. in the invention, the total viable count of the microbial composite microbial agent is preferably more than or equal to 5 multiplied by 10 9 And/g. The source of the strain used in the invention is not particularly limited, and the strain can be obtained by adopting the conventional commercial strain in the field or by self-separation from the residual sludge.
The invention also provides an enzyme preparation for treating the excess sludge, wherein the enzyme preparation comprises protease, cellulase, amylase and lysozyme; the mass ratio of the protease, the cellulase, the amylase and the lysozyme is (4-6): (2-4): (2-4): (6 to 8), preferably 5:3:3:7. in the present invention, the amylase is preferably an alpha-amylase and the protease is preferably an alkaline protease or a neutral protease. The sources of the protease, the cellulase, the amylase and the lysozyme are not particularly limited, and the protease, the cellulase, the amylase and the lysozyme can be commercially available products which are conventional in the field.
The invention also provides a method for treating the excess sludge, which comprises the following steps: 1) Dehydrating the residual sludge until the water content is 70-80%, and mixing the residual sludge with a surfactant to obtain pretreated sludge; 2) Ultrasonic treatment is carried out on the pretreated sludge to obtain sludge to be fermented; 3) Mixing the microbial composite bacterial agent with the sludge to be fermented to perform fermentation treatment to obtain fermented sludge; 4) Treating the fermented sludge by ultrasonic waves, and then mixing the fermented sludge with the enzyme preparation for enzymolysis to obtain the sludge after enzymolysis; 5) And (3) carrying out solid-liquid separation on the sludge after enzymolysis, and drying solid matters.
In the invention, the residual sludge is dehydrated to the water content of 70-80%, and then is mixed with a surfactant to obtain the pretreated sludge. In the invention, the surplus sludge is preferably the surplus sludge from a secondary sedimentation tank of a sewage treatment plant, the water content of the sludge is 96-98%, and in the invention, the dewatering is preferably performed by using a belt type dewatering filter press or a centrifuge. According to the invention, after dehydration, the surplus sludge is mixed with a surfactant, wherein the surfactant is preferably rhamnolipid, and the mass ratio of the surfactant to the surplus sludge is preferably 1: (100 to 300), more preferably 1: (150-250); the rhamnolipid is preferably prepared into a solution and then mixed with the residual sludge, and the rhamnolipid is a biosurfactant, so that the dissolution effect of the sludge can be better improved, and no secondary pollution is caused.
After the pretreated sludge is obtained, the pretreated sludge is treated by ultrasonic waves to obtain sludge to be fermented. In the present invention, the intensity of the ultrasonic treatment is preferably 0.5 to 1.0W/ml, more preferably 0.6 to 0.9W/ml, and the time of the ultrasonic treatment is preferably 10 to 15 minutes, more preferably 11 to 14 minutes. According to the invention, the ultrasonic treatment can destroy the floccule structure of the sludge, and simultaneously destroy the cell structure of the original microorganism in the sludge, so that harmful microorganisms in the sludge are killed, and the stability of the subsequent microorganism composite microbial agent fermentation environment is facilitated.
In the invention, the microbial composite bacterial agent and the sludge to be fermented are mixed for fermentation treatment to obtain the fermented sludge. In the invention, the mass ratio of the microbial composite bacterial agent to the sludge to be fermented is preferably (1-5): 100, more preferably (2 to 4): 100; in the present invention, the temperature of the fermentation treatment is preferably 30 to 40 ℃, more preferably 34 to 37 ℃, and the time of the fermentation treatment is preferably 3 to 6 days, more preferably 4 to 5 days. The pH is preferably adjusted to 6.5-7.5 before the fermentation treatment, and the pH adjusting agent is not particularly limited and can be acid-base conventional in the art.
After fermented sludge is obtained, the fermented sludge is treated by ultrasonic waves; in the present invention, the intensity of the ultrasonic treatment is preferably 0.5 to 1.0W/ml, more preferably 0.6 to 0.9W/ml; the time of the ultrasonic treatment is preferably 5 to 10 minutes, more preferably 6 to 8 minutes; after the ultrasonic treatment, carrying out enzymolysis to obtain sludge after enzymolysis; the addition amount of the enzyme preparation is 3-5 wt% of the fermented sludge, and more preferably 4wt%; the enzymolysis time is preferably 3-5 hours, more preferably 4 hours; the temperature of the enzymolysis is preferably 45-50 ℃. After the sludge subjected to enzymolysis is obtained, solid-liquid separation is carried out, the method for solid-liquid separation is not particularly limited, and centrifugation or filtration can be adopted; the solid obtained by solid-liquid separation can be used as fertilizer or soil conditioner after being dried.
According to the invention, the sludge floc structure can be rapidly broken through the combination of the surfactant and the ultrasonic treatment, so that the sludge is dispersed, the surface area is increased, and the subsequent fermentation treatment and enzymolysis are more facilitated; the invention can thoroughly degrade organic matters in the sludge by the fermentation of the microbial composite microbial agent and the enzymolysis of the enzyme preparation, and the finally obtained solid matters are less and harmless and can be directly applied.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
And (3) strain:
bacillus subtilis CGMCC 1.15792, alcaligenes faecalis CGMCC 1.2908, aspergillus niger CGMCC 3.17612, bacillus stearothermophilus CGMCC 1.4856 and Bacillus amyloliquefaciens CGMCC 1.10901, wherein actinomycetes are purchased from the national standard of detection biotechnology limited company in Xinyang city (conversion variant of white actinomycetes, number 166, purchase website:http:// www.junzhong.vip/goods.phpid＝3166)。
enzyme preparation: lysozyme was purchased from sameimer feishier technologies, protease (bromelain), cellulase, amylase were purchased from Shanghai crystal pure Biotechnology, inc., alfa Ding Shiji.
Example 1
Microbial composite microbial agent:
bacillus subtilis, alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, bacillus amyloliquefaciens and actinomyces according to the following formula 8:3:3:2:7.5:6.5 mass ratio.
Enzyme preparation: protease, cellulase, amylase and lysozyme were according to 5:3:3:7 mass ratio.
Example 2
Microbial composite microbial agent:
bacillus subtilis, alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, bacillus amyloliquefaciens and actinomyces according to the following 6:2:2:2:7:6 mass ratio.
Enzyme preparation: proteases, cellulases, amylases and lysozyme were according to 2:1:1:3 mass ratio.
Example 3
Microbial composite microbial agent:
bacillus subtilis, alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, bacillus amyloliquefaciens and actinomyces according to 11:4:4:3:9:8 mass ratio.
Enzyme preparation: protease, cellulase, amylase and lysozyme according to 3:2:2:4 mass ratio.
Comparative example 1
Microbial composite microbial agent:
bacillus subtilis, alcaligenes faecalis and aspergillus niger according to 8:3:3 mass ratio.
Enzyme preparation: protease, cellulase, amylase and lysozyme were according to 5:3:3:7 mass ratio.
Comparative example 2
Microbial composite microbial agent:
bacillus subtilis, alcaligenes faecalis and actinomycetes according to the following formula 8:3:6.5 mass ratio.
Enzyme preparation: protease, cellulase, amylase and lysozyme were according to 5:3:3:7 mass ratio.
Comparative example 3
Microbial composite microbial agent:
bacillus subtilis, alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, bacillus amyloliquefaciens and actinomyces according to the following formula 8:3:3:2:7.5:6.5 mass ratio.
And (3) an enzyme-free preparation.
Experimental example 1
200 g/part of surplus sludge separated from a secondary sedimentation tank of a town sewage treatment plant, which is 18 parts in total; centrifugally dewatering until the water content is 75%, dissolving 2g of rhamnolipid in 1ml of water, mixing with the centrifuged sludge, and stirring for 10min; ultrasonic treatment, intensity of 0.8W/ml, time of 10min.
Then, for 18 samples, dividing the samples into 6 groups, marking the samples as treatment groups 1-6, repeating each group 3, respectively adding the microbial composite agents of examples 1-3 and comparative examples 1-3 into the 6 groups, and fermenting the mixture at 35 ℃ for 5 days; obtaining fermented sludge; carrying out ultrasonic treatment again, wherein the intensity is 0.8W/ml, and the time is 5min; and (3) mixing 1-5 groups of treatment with a corresponding enzyme preparation (the addition amount is 4wt% of the fermented sludge), carrying out enzymolysis for 4 hours at 50 ℃, carrying out no treatment on 6 groups of treatment, carrying out centrifugal solid-liquid separation, weighing and drying the solid.
The results of weighing the solids are shown in Table 1.
Table 1 results of weighing solids from treatment groups 1 to 6
The composition of the microbial composite microbial agent provided by the invention has obvious influence on the treatment result of the residual sludge, and the residual sludge decrement ratio is obviously reduced after part of strains are omitted; the enzymolysis step of the enzyme preparation has a more remarkable effect on the treatment of biological sludge.
Experimental example 2
200 g/part of surplus sludge separated from a secondary sedimentation tank of a town sewage treatment plant, which is 18 parts in total; then, for 18 samples, dividing the samples into 6 groups, marking the samples as treatment groups 7-12, repeating each group for 3 times, respectively adding the microbial composite agents of the examples 1-3 into the treatment groups 7-9, and carrying out treatment according to the description of the experimental example 1; the treatment group 10 omits two ultrasonic treatments; treatment group 11 omitted the first sonication and treatment group 12 omitted the addition of rhamnolipids.
The results of weighing the solids are shown in Table 2.
Table 2 results of weighing solids in treatment groups 7 to 12
Therefore, in the treatment method provided by the invention, the ultrasonic treatment and the addition of the surfactant are important for the reduction treatment of the excess sludge, and omitting any step can influence the final sludge reduction ratio.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (9)

1. The microbial composite microbial agent for treating excess sludge is characterized by comprising bacillus subtilis, alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, bacillus amyloliquefaciens and actinomycetes; the mass ratio of the bacillus subtilis to the alcaligenes faecalis to the aspergillus niger to the bacillus stearothermophilus to the bacillus amyloliquefaciens to the actinomycetes is (5-11): (2-4): (2-4): (1-3): (6-9): (5-8).
2. The microbial composite agent according to claim 1, wherein the total viable count of the microbial composite agent is not less than 5×10 9 /g。
3. An enzyme preparation for treating excess sludge, characterized in that the enzyme preparation comprises protease, cellulase, amylase and lysozyme;
the mass ratio of the protease, the cellulase, the amylase and the lysozyme is (4-6): (2-4): (2-4): (6-8).
4. A method of treating excess sludge, comprising the steps of:
1) Dehydrating the residual sludge until the water content is 70-80%, and mixing the residual sludge with a surfactant to obtain pretreated sludge;
2) Ultrasonic treatment is carried out on the pretreated sludge to obtain sludge to be fermented;
3) Mixing the microbial composite microbial agent of claim 1 or 2 with the sludge to be fermented to perform fermentation treatment to obtain fermented sludge;
4) Treating the fermented sludge by ultrasonic waves, and then mixing the sludge with the enzyme preparation of claim 3 for enzymolysis to obtain the sludge after enzymolysis;
5) And (3) carrying out solid-liquid separation on the sludge after enzymolysis, and drying solid matters.
5. The method according to claim 4, wherein the surfactant in step 1) is rhamnolipid, and the mass ratio of the surfactant to the residual sludge is 1: (100-300).
6. The method according to claim 4, wherein the intensity of the ultrasonic treatment in the step 2) is 0.5 to 1.0W/ml, and the time of the ultrasonic treatment is 10 to 15 minutes.
7. The method according to claim 4 or 6, wherein the mass ratio of the microbial composite agent to the sludge to be fermented in the step 3) is (1-5): 100.
8. the method according to claim 4, wherein the intensity of the ultrasonic treatment in the step 4) is 0.5 to 1.0W/ml, and the time of the ultrasonic treatment is 5 to 10min; the addition amount of the enzyme preparation is 3-5 wt% of the fermented sludge.
9. The method of claim 8, wherein the time for the enzymatic hydrolysis is 3-5 hours.
CN202311259007.0A 2023-09-26 2023-09-26 Microbial composite microbial agent and method for treating excess sludge Pending CN117305165A (en)

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