CN117296861A - Attractant for neoseiulus californicus - Google Patents
Attractant for neoseiulus californicus Download PDFInfo
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- CN117296861A CN117296861A CN202311012331.2A CN202311012331A CN117296861A CN 117296861 A CN117296861 A CN 117296861A CN 202311012331 A CN202311012331 A CN 202311012331A CN 117296861 A CN117296861 A CN 117296861A
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- 239000005667 attractant Substances 0.000 title claims abstract description 34
- 241001658757 Neoseiulus californicus Species 0.000 title claims abstract description 33
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 33
- 241000238876 Acari Species 0.000 claims abstract description 35
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 claims abstract description 34
- 238000012360 testing method Methods 0.000 claims abstract description 29
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 claims abstract description 17
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 claims abstract description 17
- 229930007744 linalool Natural products 0.000 claims abstract description 17
- NHTMVDHEPJAVLT-UHFFFAOYSA-N Isooctane Chemical compound CC(C)CC(C)(C)C NHTMVDHEPJAVLT-UHFFFAOYSA-N 0.000 claims abstract description 16
- JVSWJIKNEAIKJW-UHFFFAOYSA-N dimethyl-hexane Natural products CCCCCC(C)C JVSWJIKNEAIKJW-UHFFFAOYSA-N 0.000 claims abstract description 16
- BOGVTNYNTGOONP-UHFFFAOYSA-N 3,4-dihydroxyoxolane-2,5-dione Chemical compound OC1C(O)C(=O)OC1=O BOGVTNYNTGOONP-UHFFFAOYSA-N 0.000 claims description 16
- XQQVOBPJWHQXEN-UHFFFAOYSA-N undecyl-Cyclohexane Chemical compound CCCCCCCCCCCC1CCCCC1 XQQVOBPJWHQXEN-UHFFFAOYSA-N 0.000 claims description 16
- 230000009257 reactivity Effects 0.000 claims description 14
- 239000004480 active ingredient Substances 0.000 claims description 12
- 229920000742 Cotton Polymers 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 239000002985 plastic film Substances 0.000 claims description 3
- 229920006255 plastic film Polymers 0.000 claims description 3
- 230000004044 response Effects 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 230000007480 spreading Effects 0.000 claims description 2
- 241000607479 Yersinia pestis Species 0.000 abstract description 10
- 238000011161 development Methods 0.000 abstract description 6
- 230000002265 prevention Effects 0.000 abstract description 6
- 238000011160 research Methods 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000035558 fertility Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 16
- 241000196324 Embryophyta Species 0.000 description 7
- 238000010586 diagram Methods 0.000 description 5
- 241000207199 Citrus Species 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 4
- 235000020971 citrus fruits Nutrition 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 229940057995 liquid paraffin Drugs 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000851181 Eutetranychus orientalis Species 0.000 description 2
- 230000006399 behavior Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000003039 volatile agent Substances 0.000 description 2
- 241001672694 Citrus reticulata Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000000856 effect on pests Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 229920005669 high impact polystyrene Polymers 0.000 description 1
- 239000004797 high-impact polystyrene Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- ZNJFBWYDHIGLCU-HWKXXFMVSA-N jasmonic acid Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(O)=O)CCC1=O ZNJFBWYDHIGLCU-HWKXXFMVSA-N 0.000 description 1
- 229960001047 methyl salicylate Drugs 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/011—Crawling insects
Abstract
The invention relates to the technical field of biological control of predatory mites, and discloses an attractant for neoseiulus californicus, which comprises a test device, wherein the test device uses a Y-type olfactometer, and the concentration of linalool is 10 4 ug/mL and 10 3 ug/mL, the selection quantity is 50 and 65, the selection rate is 55.56 percent and 68.42 percent, the reaction rate is 90 percent and 95 percent, and the chi-square value is 1.11 and 12.9; isooctane concentration of 10 4 ug/mL and 10 3 ug/mL, 56 and 59, 60.87% and 64.13% selectivity, 92% reaction rate, and 4.35 and 7.35 chi-square. In the invention, the neoseiulus californicus is used for pest mite generationThe novel small-seiulus californicus has the advantages that the use of the attractant is not seen, the development of the novel small-seiulus californicus is fast, the fertility is high, the predatory quantity is large, the large-scale feeding technology is mature, the novel small-seiulus californicus is a kind with higher commercial application degree in predatory mites, the research of the attractant for the novel small-seiulus californicus is helpful for improving the prevention and control efficiency of the predatory mites, one of the key problems to be considered in national green prevention and control is realized, and the requirements of national green agriculture sustainable development are met.
Description
Technical Field
The invention relates to the technical field of biological control of predatory mites, in particular to an attractant for neoseiulus californicus.
Background
The phytophagostimulant mites are important worldwide pests, damage to crops, fruit trees, ornamental plants and the like, the phytophagostimulant mites cause leaf tissue damage and color change by piercing plant leaf juice, release of HIPVs such as terpenoids, green Leaf Volatiles (GLV), jasmonate, methyl salicylate and the like is induced, the citrus Citrus reticulata Blanco is used as the first bulk fruit in the world, the first place of global fruit trade is the most serious world pest mites of citrus, chemical control is a main measure for controlling the harm of the world pest mites, but due to the characteristics of small size, rapid propagation, wide distribution, strong adaptability, easiness in drug resistance and the like, the biological control of pesticide residues, drug resistance and rampant '3R' problems caused by chemical control is particularly important, the citrus is high in egg laying range of new small citrus mites in california, wide in application range of the application of the citrus, has great biological control value on the chemical control of the citrus pest mites, and has the application of the agricultural pest mites to various agricultural mites.
The three-stage nutrition relationship of plant, insect and natural enemy is a hotspot in research of insect chemical ecology, and is also an important basis for searching for sustainable green prevention and control of insect pests, so that the development and utilization of natural enemy as urban agricultural service are consensus, the prevention and control of agricultural mites by utilizing natural enemy predatory mites is an important measure for biological prevention and control, and has positive effects on the development of agriculture reduction and green agriculture, but in view of higher natural enemy cost, how to increase the utilization efficiency of natural enemy becomes a problem to be solved urgently, and recent research shows that the plant predatory mites take a plant-induced HIPVs (herervore-induced plant volatiles) to play a key role in regulating interaction of three-stage nutrition relationship of plant, insect and predatory mites, so that the research of new small new california has a key role in inducing action of Vs on the citrus mites, the identification of efficient attractant is helpful for attracting the predatory mites to enhance the pest control capability of the HIPmite. For this purpose we propose an attractant for neoseiulus californicus.
Disclosure of Invention
The invention mainly solves the technical problems existing in the prior art and provides an attractant for neoseiulus californicus.
In order to achieve the aim, the invention adopts the following technical scheme that the attractant for neoseiulus californicus comprises a test device, wherein the test device uses a Y-type olfactometer, and the concentration of linalool is 10 4 ug/mL and 10 3 ug/mL, 50 and 65, 55.56% and 68.42% selectivity, 90% and 95% reactivity, chi-square values of 1.11 and 12.9, significance "none" and "; isooctane concentration of 10 4 ug/mL and 10 3 ug/mL, 56 and 59, 60.87% and 64.13% selectivity, 92% reaction rate, and chi-square values of 4.35 and 7.35, with significance of "sum"; the concentration of undecylcyclohexane was 10 4 ug/mL and 10 3 ug/mL, 58 and 61, 62.36% and 64.89% selectivity, 93% and 94% reactivity, chi-square values of 5.69 and 8.34, significance ". Times.and"; tartaric anhydride concentration of 10 4 ug/mL and 10 3 ug/mL, 61 and 60, 63.54% and 65.93% selectivity, 96% and 91% response, and chi-square values of 7.042 and 8.522, significance being "×" and "×".
Preferably, the optimal concentration of linalool is selected to be 56 and 61, the selectivity is 60.22% and 63.5%, the reaction rate is 93% and 96%, the chi-square value is 3.88 and 7.04, and the significance is ". Times"; the optimum concentration of isooctane was selected to be 55, 60 and 62 compared to the other concentrations, the selectivities 60.44%, 63.83% and 64.58%, the reactivities 91%, 94% and 96%, the chi-square values 3.97, 7.19 and 8.17, and the significance of ",".
Preferably, the optimal undecylcyclohexane concentration is selected to be 37, 57 and 59, the selectivity is 39.78%, 60.64% and 64.13%, the reaction rate is 93%, 94% and 92%, the chi-square value is 3.88, 4.74 and 7.35, and the significance is ", and"; the optimal concentration of tartaric anhydride was chosen as 58, 59 and 60 compared to the other concentrations, the selectivities 60.42%, 62.11% and 63.83%, the reactivities 96%, 95% and 94%, the chi-square values 4.17, 5.57 and 7.19, and the significance of "," "and".
Preferably, the combination of the two different active ingredients of linalool, isooctane, undecylcyclohexane and tartaric anhydride is selected from 43, 57, 45, 58, 50 and 56, the selectivity is 45.26%, 60.64%, 47.87%, 61.7%, 52.08% and 60.22%, and the reaction rate is 95%, 94%, 96% and 93%.
Preferably, the chi-square value of the combination of the two different active ingredients of linalool, isooctane, undecylcyclohexane, tartaric anhydride is 0.85, 4.26, 0.17, 5.15, 0.17 and 3.88, with significance of "none", "none" and ". Times..
Preferably, the combination of the 3 different active ingredients of linalool, isooctane, undecylcyclohexane and tartaric anhydride is selected to be 46, 58, 50 and 48, the selectivity is 50.00%, 61.05%, 53.19% and 51.61%, the reaction rate is 92%, 95%, 94% and 93%, and the chi-square value is 0, 4.64, 0.383 and 0.097, and the significance is "none", "no" and "no".
Preferably, the test device is used as follows: uniformly covering a layer of absorbent cotton on the bottom of a glass culture dish with the diameter of 15cm, adding distilled water to keep wet, uniformly spreading a round black plastic film with the diameter of 13cm on the absorbent cotton, dividing the film into two areas with equal area by using a color pen with the diameter as a dividing line, and placing round filter paper on the symmetrical positions of the diameter.
Preferably, the number of the round filter paper sheets is two, the diameter of the round filter paper sheets is 0.5cm, the test is two groups, the test time is 10min, each group of tests releases 100 new small mites in California, the left side is a control area, 33 new small mites in california gather, the right side is an attractant area, 65 new small mites in california gather, and the attractant area accounts for 66 percent.
Advantageous effects
The invention provides an attractant for neoseiulus californicus. The beneficial effects are as follows:
(1) The biological control of mites by using the neoseiulus californicus has the advantages that no attractant is used, the neoseiulus californicus has fast development, high fertility and large predation, the large-scale feeding technology is mature, the method is a kind with higher commercial application degree in predatory mites, the research on the neoseiulus californicus attractant is helpful for improving the control efficiency of the predatory mites, and the method is one of the key problems of national green prevention and control consideration, and meets the requirements of national green agriculture sustainable development.
(2) Compared with liquid paraffin without the effective components, the attractant has the trapping rate of 66 percent on neoseiulus californicus, which is obviously higher than that of the liquid paraffin without the effective components.
(3) The traditional attractant is generally a pest attractant, has a trapping effect on pests, belongs to natural enemy attractants, can obviously improve the use efficiency of commercial natural enemies, reduces the use cost of the natural enemies, has strong specificity and is environment-friendly, and the effective components of the attractant are derived from commercially synthesized pest-induced volatile (HIPVs).
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those skilled in the art from this disclosure that the drawings described below are merely exemplary and that other embodiments can be derived from the drawings provided without the inventive effort.
The structures, proportions, sizes, etc. shown in the present specification are shown only for the purposes of illustration and description, and are not intended to limit the scope of the invention, which is defined by the claims, so that any structural modifications, changes in proportions, or adjustments of sizes, which do not affect the efficacy or the achievement of the present invention, should fall within the ambit of the technical disclosure.
FIG. 1 is a schematic diagram of an embodiment of the present invention;
FIG. 2 is a schematic diagram of a second embodiment of the present invention;
FIG. 3 is a schematic diagram of a third embodiment of the present invention;
FIG. 4 is a schematic diagram of a fourth embodiment of the present invention;
fig. 5 is a schematic diagram of an embodiment of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Embodiment one: an attractant for new small Calseiid mites in California, as shown in figure 1, comprises an experimental device, wherein the experimental device uses a Y-shaped olfactometer, a single-end female small Calseiid mites to be detected is connected to a filament wire at the air outlet end of the Y-shaped olfactometer by a writing brush, the behavioral response of the mites to two odor sources within 5min after connection is observed and recorded, when the mites select a certain tube wall direction and crawl more than 2/3 of the filament wire for more than 3 seconds, the effect is recorded, the selection is recorded as the corresponding odor sources, if the mites do not select or crawl repeatedly and stay for a long time within 5min, the new small Calseiid mites in California are only used for one olfactory selection test, the Y-shaped olfactometer is replaced and the odor source direction is replaced every 10 new small California mites are tested, the test is performed for 100 heads, screening of single active ingredients, and a plurality of pre-experiments prove that the gas flow rate (300 mL/min) which is suitable for the neoseiulus californicus is found to be high in selection efficiency on higher volatile matter concentrations (10.00 mg/mL and 1.00 mg/mL) before the experiment is carried out, so that the experiment uses Paraffin liquid (Macklin) as a solvent to prepare a single HIPVs solution with the mass concentration of 10.00mg/mL and the mass concentration of 1.00mg/mL as an odor source for screening HIPVs with the attraction effect on the neoseiulus californicus, the Paraffin liquid is used as a control odor source, and each concentration selects 100 neoseiulus californicus female mites as a test object, and the single active ingredient with the attraction effect on the neoseiulus californicus screened from 20 artificially synthesized HIPS components is shown in a table of fig. 1.
Linalool concentration of 10 4 ug/mL and 10 3 ug/mL, 50 and 65, 55.56% and 68.42% selectivity, 90% and 95% reactivity, chi-square values of 1.11 and 12.9, significance "none" and "; isooctane concentration of 10 4 ug/mL and 10 3 ug/mL, 56 and 59, 60.87% and 64.13% selectivity, 92% reaction rate, and chi-square values of 4.35 and 7.35, with significance of "sum"; the concentration of undecylcyclohexane was 10 4 ug/mL and 10 3 ug/mL, 58 and 61, 62.36% and 64.89% selectivity, 93% and 94% reactivity, chi-square values of 5.69 and 8.34, significance ". Times.and"; tartaric anhydride concentration of 10 4 ug/mL and 10 3 ug/mL, 61 and 60, 63.54% and 65.93% selectivity, 96% and 91% response, and chi-square values of 7.042 and 8.522, significance being "×" and "×".
Note that: * Indicating significant differences (P < 0.05), indicating very significant differences (P < 0.01) (chi-square goodness of fit test, the same applies below).
Embodiment two: as shown in fig. 2, 4 single effective components having an attracting effect on new small-seiulus californicus are prepared into four mass concentration gradient solutions of 10mg/mL, 1mg/mL, 0.1mg/mL and 0.01mg/mL respectively by using liquid paraffin as a solvent, wherein each group of tests selects 100 new small-seiulus californicus female mites as test objects, in order to screen out the concentration of the HIPVs with the best attracting effect, the test group design is optimized, after each group of tests is finished, the concentration of the HIPVs with the good attracting effect is taken as one of odor sources of the next group of tests, the HIPVs with poor attracting effect is excluded from the subsequent test design, the test finally obtains the best concentration of each effective component, the concentrations are expressed by capital letters instead of other non-best concentration lower case letters, letters "a" represent linalool with the best attracting effect concentration, "B" represent isooctane with the best attracting effect concentration, "C" represent undecyl cyclohexane with the best attracting effect, "and letters" represent tartaric anhydride with the best attracting effect "with the best attracting effect.
The optimal concentration of linalool is selected to be 56 and 61, the selectivity is 60.22% and 63.5%, the reaction rate is 93% and 96%, the chi-square value is 3.88 and 7.04, and the significance is ". Times"; the optimum concentration of isooctane was selected to be 55, 60 and 62 compared to the other concentrations, the selectivities were 60.44%, 63.83% and 64.58%, the reactivities were 91%, 94% and 96%, the chi-square values were 3.97, 7.19 and 8.17, the significance was "," and "+," the optimum concentration of undecylcyclohexane was selected to be 37, 57 and 59 compared to the other concentrations, the selectivities were 39.78%, 60.64% and 64.13%, the reactivities were 93%, 94% and then 92%, the chi-square values were 3.88, 4.74 and 7.35, the significance was "," "and"; the optimal concentration of tartaric anhydride was chosen as 58, 59 and 60 compared to the other concentrations, the selectivities 60.42%, 62.11% and 63.83%, the reactivities 96%, 95% and 94%, the chi-square values 4.17, 5.57 and 7.19, and the significance of "," "and".
Note that: * Indicating significant difference (P < 0.05), indicating very significant difference (P < 0.01); A. b, C, D represents the optimal attracting concentration of each active ingredient, and a, b, c represent the other concentrations in the test
Embodiment III: as shown in FIG. 3, the attracting effect of the neoseiulus californicus is probably not just that of a certain HIPVs, and therefore, a behavior selection test of the neoseiulus californicus on the combination of two effective components is designed, and the attracting effect of the neoseiulus californicus on the combination of two effective components is verified by 6 test groups (A+Bvs A, A+Cvs A, A+Dvs A, B+Cvs B, B+Dvs B, C+Dvs C).
The combination of two different active ingredients of linalool, isooctane, undecylcyclohexane, and tartaric anhydride was selected as 43, 57, 45, 58, 50, and 56, with selectivity of 45.26%, 60.64%, 47.87%, 61.7%, 52.08%, and 60.22%, reaction rates of 95%, 94%, 96%, and 93%, and chi-square values of 0.85, 4.26, 0.17, 5.15, 0.17, and 3.88, with significance of "none", and ".
Note that: * Indicating significant difference (P < 0.05), indicating very significant difference (P < 0.01); A. b, C, D represents the optimal attracting concentration of each active ingredient, respectively.
Embodiment four: as shown in fig. 4, the attractant combination linalool, undecylcyclohexane, and tartaric anhydride was selected by verifying the attracting effect of 3 active ingredients on neoseiulus californicus by 4 test groups (a+b+c vs a+c, a+c+d vs a+c, b+c+d+d+c, a+c+d+vs a+b+c+d).
The selection of the combination of different 3 active ingredients of linalool, isooctane, undecylcyclohexane, tartaric anhydride was 46, 58, 50 and 48, the selectivity was 50.00%, 61.05%, 53.19% and 51.61%, the reaction rates were 92%, 95%, 94% and 93%, the chi-square values were 0, 4.64, 0.383 and 0.097, and the significance was "none", "none" and "none".
Note that: * Indicating significant difference (P < 0.05)
Fifth embodiment: as shown in FIG. 5, a layer of absorbent cotton is uniformly covered on the bottom of a glass culture dish with the diameter of 15cm, distilled water is added to keep wet, a round black plastic film with the diameter of 13cm is uniformly laid on the absorbent cotton, a color pen is used for dividing the film into two areas with equal areas by taking the diameter as a dividing line, 2 round filter paper sheets with the diameter of 0.5cm are placed at symmetrical positions of the diameter, liquid paraffin and an attractant are respectively dripped on the filter paper sheets, A circular area with the diameter of 1cm is drawn at the center of the film and is used as a release point of the neoseiulus californicus, 100 neoseiulus californicus is released in each group of experiments, and the numbers of mites distributed in the two areas are observed and counted by photographing after 10 minutes, as shown in figure 5, 33 neoseiulus californicus is gathered in the left side of the control area, 65 neoseiulus californicus is gathered in the right side of the control area, and the attractant area accounts for 66%.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. The attractant for the neoseiulus californicus comprises a test device and is characterized in that: the test device uses a Y-type olfactometer, and the concentration of linalool is 10 4 ug/mL and 10 3 ug/mL, 50 and 65, 55.56% and 68.42% selectivity, 90% and 95% reactivity, chi-square values of 1.11 and 12.9, significance "none" and "; isooctane concentration of 10 4 ug/mL and 10 3 ug/mL, 56 and 59, 60.87% and 64.13% selectivity, 92% reaction rate, and chi-square values of 4.35 and 7.35, with significance of "sum"; the concentration of undecylcyclohexane was 10 4 ug/mL and 10 3 ug/mL, 58 and 61, 62.36% and 64.89% selectivity, 93% and 94% reactivity, chi-square values of 5.69 and 8.34, significance ". Times.and"; tartaric anhydride concentration of 10 4 ug/mL and 10 3 ug/mL, 61 and 60, 63.54% and 65.93% selectivity, 96% and 91% response, and chi-square values of 7.042 and 8.522, significance being "×" and "×".
2. An attractant for neoseiulus californicus as claimed in claim 1, wherein: the optimal concentration of linalool is 56 and 61 compared with other concentrations, the selectivity is 60.22% and 63.5%, the reaction rate is 93% and 96%, the chi-square value is 3.88 and 7.04, and the significance is "x" and "x"; the optimum concentration of isooctane was selected to be 55, 60 and 62 compared to the other concentrations, the selectivities 60.44%, 63.83% and 64.58%, the reactivities 91%, 94% and 96%, the chi-square values 3.97, 7.19 and 8.17, and the significance of ",".
3. An attractant for neoseiulus californicus as claimed in claim 1, wherein: the optimal undecylcyclohexane concentration was chosen to be 37, 57 and 59 compared to the other concentrations, selectivities 39.78%, 60.64% and 64.13%, reactivities 93%, 94% and 92%, chi-square values 3.88, 4.74 and 7.35, significance ", and"; the optimal concentration of tartaric anhydride was chosen as 58, 59 and 60 compared to the other concentrations, the selectivities 60.42%, 62.11% and 63.83%, the reactivities 96%, 95% and 94%, the chi-square values 4.17, 5.57 and 7.19, and the significance of "," "and".
4. An attractant for neoseiulus californicus as claimed in claim 1, wherein: the combination of two different effective components of linalool, isooctane, undecylcyclohexane and tartaric anhydride is selected as 43, 57, 45, 58, 50 and 56, the selectivity is 45.26%, 60.64%, 47.87%, 61.7%, 52.08% and 60.22%, and the reaction rate is 95%, 94%, 96% and 93%.
5. An attractant for neoseiulus californicus as claimed in claim 4, wherein: the chi-square values of the different two active ingredient combinations of linalool, isooctane, undecylcyclohexane, tartaric anhydride are 0.85, 4.26, 0.17, 5.15, 0.17 and 3.88, with significance of "none", "no", "none" and "x".
6. An attractant for neoseiulus californicus as claimed in claim 1, wherein: the selection of the combination of the different 3 active ingredients of linalool, isooctane, undecylcyclohexane and tartaric anhydride is 46, 58, 50 and 48, the selectivity is 50.00%, 61.05%, 53.19% and 51.61%, the reaction rate is 92%, 95%, 94% and 93%, and the chi-square value is 0, 4.64, 0.383 and 0.097, and the significance is "none", "no", "none" and "none".
7. An attractant for neoseiulus californicus as claimed in claim 1, wherein: the using method of the test device comprises the following steps: uniformly covering a layer of absorbent cotton on the bottom of a glass culture dish with the diameter of 15cm, adding distilled water to keep wet, uniformly spreading a round black plastic film with the diameter of 13cm on the absorbent cotton, dividing the film into two areas with equal area by using a color pen with the diameter as a dividing line, and placing round filter paper on the symmetrical positions of the diameter.
8. An attractant for neoseiulus californicus as claimed in claim 7, wherein: the number of the round filter paper sheets is two, the diameter of the round filter paper sheets is 0.5cm, the test is two groups, the test time is 10min, each group of test releases 100 new small mites in California, the left side is a control area, 33 new small mites in california gather, the right side is an attractant area, 65 new small mites in california gather, and the attractant area accounts for 66 percent.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311012331.2A CN117296861A (en) | 2023-08-11 | 2023-08-11 | Attractant for neoseiulus californicus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311012331.2A CN117296861A (en) | 2023-08-11 | 2023-08-11 | Attractant for neoseiulus californicus |
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| CN202311012331.2A Pending CN117296861A (en) | 2023-08-11 | 2023-08-11 | Attractant for neoseiulus californicus |
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| Country | Link |
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2023
- 2023-08-11 CN CN202311012331.2A patent/CN117296861A/en active Pending
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