CN117288749A - Reagent and method for rapidly judging slow release performance of sodium butyrate - Google Patents
Reagent and method for rapidly judging slow release performance of sodium butyrate Download PDFInfo
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- MFBOGIVSZKQAPD-UHFFFAOYSA-M sodium butyrate Chemical compound [Na+].CCCC([O-])=O MFBOGIVSZKQAPD-UHFFFAOYSA-M 0.000 title claims abstract description 71
- 238000000034 method Methods 0.000 title claims abstract description 30
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 23
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 claims abstract description 27
- ZPLCXHWYPWVJDL-UHFFFAOYSA-N 4-[(4-hydroxyphenyl)methyl]-1,3-oxazolidin-2-one Chemical compound C1=CC(O)=CC=C1CC1NC(=O)OC1 ZPLCXHWYPWVJDL-UHFFFAOYSA-N 0.000 claims abstract description 22
- OLQIKGSZDTXODA-UHFFFAOYSA-N 4-[3-(4-hydroxy-2-methylphenyl)-1,1-dioxo-2,1$l^{6}-benzoxathiol-3-yl]-3-methylphenol Chemical compound CC1=CC(O)=CC=C1C1(C=2C(=CC(O)=CC=2)C)C2=CC=CC=C2S(=O)(=O)O1 OLQIKGSZDTXODA-UHFFFAOYSA-N 0.000 claims abstract description 22
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 22
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 22
- 239000011734 sodium Substances 0.000 claims abstract description 22
- 239000000243 solution Substances 0.000 claims description 61
- 230000008859 change Effects 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 16
- 239000011259 mixed solution Substances 0.000 claims description 11
- 238000013268 sustained release Methods 0.000 claims description 11
- 239000012730 sustained-release form Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000011156 evaluation Methods 0.000 claims description 6
- 229960003531 phenolsulfonphthalein Drugs 0.000 claims description 5
- 238000001514 detection method Methods 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 230000000968 intestinal effect Effects 0.000 description 6
- 230000029087 digestion Effects 0.000 description 5
- 238000004090 dissolution Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 102000038379 digestive enzymes Human genes 0.000 description 2
- 108091007734 digestive enzymes Proteins 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012946 outsourcing Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000015067 sauces Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 101150038956 cup-4 gene Proteins 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000013538 functional additive Substances 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 230000007236 host immunity Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 210000002490 intestinal epithelial cell Anatomy 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/15—Medicinal preparations ; Physical properties thereof, e.g. dissolubility
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
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Abstract
The invention provides a reagent and a method for rapidly judging the slow release performance of sodium butyrate, wherein the reagent for rapidly judging the slow release performance of sodium butyrate comprises the following components: bromothymol blue sodium solution, m-cresol purple solution, phenol red solution and phenolphthalein solution. Compared with the existing method, the reagent and the method provided by the invention are simple, and the method is simple to operate, fast in speed and low in cost.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a reagent and a method for rapidly judging the slow release performance of sodium butyrate.
Background
Sodium butyrate can improve the morphological structure and the function of intestinal tracts, promote the differentiation of intestinal epithelial cells of livestock and poultry animals, and improve the digestion and absorption efficiency of nutrient substances. Sodium butyrate can also stimulate intestinal mucosa to secrete immunoglobulin, promote proliferation and differentiation of immune cells, regulate B and T cell functions in the later period of antigen exposure, and regulate host immunity; sodium butyrate also increases digestibility by increasing digestive enzyme activity, etc. In addition, in the aspect of regulating intestinal microecology, sodium butyrate can reduce the quantity of escherichia coli, promote the reproduction of lactobacillus, increase the diversity of intestinal flora and maintain the intestinal microecology balance. Therefore, the sodium butyrate is used as a functional additive and is widely applied to livestock and poultry feeds.
Sodium butyrate is a small molecular substance and can be absorbed and act in the gastrointestinal tract of livestock and poultry, so that a plurality of commercial products are called as slow release effect and can be released in the whole digestive tract. The conventional method for detecting the slow release performance of sodium butyrate is to simulate animal digestion in vitro by using a dissolution tester, sample the sodium butyrate according to a time period and detect the sodium butyrate by using high performance liquid chromatography. This method is well quantified, but long for detection, expensive reagents are used, and precise instrumentation is required, which is difficult for small feed enterprises to accomplish.
Therefore, a reagent and a method with low cost are required to be developed, and the slow release performance of sodium butyrate can be simply and rapidly judged.
Disclosure of Invention
Based on the expression, the invention provides a reagent and a method for rapidly judging the sodium butyrate slow release performance, which are used for rapidly judging the sodium butyrate slow release performance.
The technical scheme for solving the technical problems is as follows:
in a first aspect, the present invention provides a reagent for rapid evaluation of sodium butyrate sustained release performance, comprising: bromothymol blue sodium solution, m-cresol purple solution, phenol red solution and phenolphthalein solution.
Based on the technical scheme, the invention can be improved as follows.
Further, the mixing ratio of the bromothymol blue sodium solution, the m-cresol purple solution, the phenol red solution and the phenolphthalein solution is 2-4: 4 to 5:0.01 to 1:1 to 2.
Further, the concentration of the bromothymol blue sodium solution, the m-cresol purple solution, the phenol red solution and the phenolphthalein solution is respectively 0.2-0.7 g/L, 0.8-1.3 g/L, 0.5-0.3 g/L and 0.5-2.0 g/L.
Further, the concentration of the bromothymol blue sodium solution, the meta-cresol purple solution, the phenol red solution and the phenolphthalein solution are 0.5g/L, 1.0g/L, 0.2g/L and 1.0g/L, respectively.
In a second aspect, the present invention provides a method for evaluating sodium butyrate sustained release performance using the reagent for rapidly evaluating sodium butyrate sustained release performance as described in the first aspect, comprising the steps of:
s1, mixing four solutions of bromothymol blue sodium, m-cresol purple, phenol red and phenolphthalein in proportion, and obtaining a bright orange mixed solution after mixing;
s2, adding 5-8 drops of the mixed solution into water to obtain a light yellow water sample;
s3, adding a sodium butyrate sample into the water sample, and shaking uniformly;
and S4, observing the color change condition of the solution within two hours, and judging the slow release effect of the sodium butyrate sample according to the color change condition of the solution.
Based on the technical scheme, the invention can be improved as follows.
Further, in the step S1, the mixing ratio of the four solutions of bromothymol blue sodium, m-cresol purple, phenol red and phenolphthalein is 2-4: 4 to 5:0.01 to 1:1 to 2.
Further, in step S3, the effective content of sodium butyrate in the sodium butyrate sample is 1.5 to 2.5g.
Further, in step S4, the determining the slow release effect of the sodium butyrate sample according to the color change condition of the solution specifically includes:
when the color of the solution changes from yellow to green, then from green to blue and finally from blue to purple, the sodium butyrate sample has a slow release effect;
when the solution is light yellow or light green and has no change, the sodium butyrate sample cannot be released, and the sodium butyrate sample has no slow release effect;
when the solution was blue or purple and there was no change, it was shown that the sodium butyrate sample was completely released and the sodium butyrate sample had no slow release effect.
Compared with the prior art, the technical scheme of the application has the following beneficial technical effects:
the traditional existing sodium butyrate slow release performance evaluation method is a method for simulating animal digestion in vitro, the detection time is 12-16 hours, the detection process needs to use digestive enzymes extracted from the animal body with high price, and meanwhile, a precise instrument high performance liquid chromatography is needed. Compared with the existing method, the reagent and the method provided by the invention are simple, and the method is simple to operate, fast in speed and low in cost.
Drawings
Fig. 1 is a schematic diagram of experimental results of comparative examples provided by the present invention.
Detailed Description
Embodiments of the present solution will be described in further detail below. It is clear that the described embodiments are only some of the embodiments of the present solution, not an exhaustive list of all embodiments. It should be noted that, in the case of no conflict, the embodiments and features in the embodiments in the present solution may be combined with each other.
Modifications to the disclosure of the invention may be made in the materials, methods, and reaction conditions, all of which are intended to fall within the spirit and scope of the invention. The technical means used in the examples are conventional means well known to those skilled in the art unless otherwise indicated. The reagents and biological materials, unless otherwise specified, are commercially available; the databases used are all public online databases.
Example 1
The embodiment provides a reagent for rapidly judging the slow release performance of sodium butyrate, which comprises a bromothymol blue sodium solution, a metacresol purple solution, a phenol red solution and a phenolphthalein solution.
Specifically, 0.5g/L bromothymol blue sodium solution, 1.0g/L m-cresol purple solution, 0.2g/L phenol red solution and 1.0g/L phenolphthalein solution are mixed according to a ratio of 4:5:0.01:1, and uniformly mixing the components.
Example 2
The embodiment provides a reagent for rapidly judging the slow release performance of sodium butyrate, which comprises a bromothymol blue sodium solution, a metacresol purple solution, a phenol red solution and a phenolphthalein solution.
Specifically, 0.5g/L bromothymol blue sodium solution, 1.0g/L m-cresol purple solution, 0.2g/L phenol red solution and 1.0g/L phenolphthalein solution are mixed according to a ratio of 3:4:1:2, and uniformly mixing the components.
Example 3
The embodiment provides a reagent for rapidly judging the slow release performance of sodium butyrate, which comprises a bromothymol blue sodium solution, a metacresol purple solution, a phenol red solution and a phenolphthalein solution.
Specifically, 0.5g/L bromothymol blue sodium solution, 1.0g/L m-cresol purple solution, 0.2g/L phenol red solution and 1.0g/L phenolphthalein solution are mixed according to a ratio of 2:5:1:2, and uniformly mixing the components.
Example 4
The embodiment provides a reagent for rapidly judging the slow release performance of sodium butyrate, which comprises a bromothymol blue sodium solution, a metacresol purple solution, a phenol red solution and a phenolphthalein solution.
Specifically, 0.5g/L bromothymol blue sodium solution, 1.0g/L m-cresol purple solution, 0.2g/L phenol red solution and 1.0g/L phenolphthalein solution are mixed according to a ratio of 3:5:0.5: 1.5.
Example 5
The embodiment provides a method for rapidly judging the slow release performance of sodium butyrate, which comprises the following steps:
the method comprises the following steps:
s1, mixing four solutions of bromothymol blue sodium, m-cresol purple, phenol red and phenolphthalein in proportion, and obtaining a bright orange mixed solution after mixing.
S2, adding 5-8 drops of the mixed solution into the water to obtain a light yellow water sample.
S3, adding a sodium butyrate sample into the water sample, and shaking uniformly; wherein the effective content of sodium butyrate in the sodium butyrate sample is 1.5-2.5 g.
S4, observing the color change condition of the solution within two hours, and judging the slow release effect of the sodium butyrate sample according to the color change condition of the solution:
when the color of the solution changes from yellow to green, then from green to blue and finally from blue to purple, the sodium butyrate sample has a slow release effect;
when the solution is light yellow or light green and has no change, the sodium butyrate sample cannot be released, and the sodium butyrate sample has no slow release effect;
when the solution was blue or purple and there was no change, it was shown that the sodium butyrate sample was completely released and the sodium butyrate sample had no slow release effect.
Specifically, a total of 5 sodium butyrate samples were provided for rapid evaluation of sustained release performance:
sample 1: a laboratory self-made sample with 40% sodium butyrate;
sample 2: the content of sodium butyrate in the sauce product 90B is 90%;
sample 3: the content of sodium butyrate is 68% in the sauce product double Ding Bao;
sample 4: outsourcing products, wherein the sodium butyrate content is 30%;
sample 5: outsourcing products, wherein the sodium butyrate content is 50%.
The mixed solutions prepared in examples 1 to 4 above were used for the following experiments:
(1) Taking 20 triangular flasks with the number of 1-20, and respectively adding 50ml of tap water;
(2) 6 drops of the mixed solution of example 1 were added to Erlenmeyer flasks 1-5;
6 drops of the mixed solution of example 2 were added to Erlenmeyer flasks 6-10;
6 drops of the mixed solution of example 3 were added to Erlenmeyer flasks 11-15;
6 drops of the mixed solution of example 4 were added to the flask 16-20;
(3) 5.0g of sample 1 was added to each of the flasks 1, 6, 11, 16;
2.0g of sample 2 was added to each of the flasks 2, 7, 12, 17;
3.0g of sample 3 was added to each of the flasks 3, 8, 13, 18;
6.5g of sample 4 was added to each of the flasks 4, 9, 14, 19;
4.0g of sample 5 was added to each of the flasks 5, 10, 15, 20;
the 20 triangular flasks were shaken well and observed for 2h.
The color change over all samples 2h is shown in table 1.
Table 1 color change table within 2h for each sample
Comparative example
The slow release performance of 5 samples was evaluated using a conventional in vitro simulated digestion method, the experimental procedure was as follows:
(1) Preparing simulated gastric juice: pepsin was weighed and dissolved in hydrochloric acid solution at pH3.5 to a concentration of 2.1g/L.
(2) Preparing simulated intestinal juice: weighing pancreatin dissolved in 1% NaHCO 3 The concentration of the solution was brought to 2.5g/L.
(3) The drug dissolution tester is started, the water bath temperature is 39 ℃, and the stirring rotating speed is 100r/min.
(4) 400ml simulated gastric fluid was poured into each of the dissolution cups 1-5, and after 10min, 1g sample 1 was added to cup 1, 1g sample 2 was added to cup 1, 1g sample 3 was added to cup 3, 1g sample 4 was added to cup 4, and 1g sample 5 was added to cup 5. (samples 1 to 5 are identical to samples 1 to 5 in example 5 above and will not be described here again
(5) Timing was started from the time of sample addition, and samples were taken at 1h and 2h after the reaction, respectively.
(6) After taking the second sample, K is used 2 HPO 4 The pH of the simulated gastric fluid in the dissolution cup was adjusted to 6.5-6.7 and 8mL of simulated intestinal fluid was added.
(7) Samples were taken 1h, 2h, 4h, 8h after addition of simulated intestinal fluid.
(8) The removed samples were tested using high performance liquid chromatography.
The detection result is shown in fig. 1, and according to the detection result shown in fig. 1, it is known that the sample 1 cannot be released and has no slow release performance; sample 2 released rapidly, without slow release properties; sample 3, sample 4 and sample 5 all had slow release properties, and the results were consistent with the trend of the slow release results of example 5.
In combination with the above examples and comparative examples, it can be seen that the examples of the present invention have the following advantages:
the traditional existing sodium butyrate slow release performance evaluation method is a method for simulating animal digestion in vitro, the detection time is 12-16 hours, the detection process needs to use digestive enzymes extracted from the animal body with high price, and meanwhile, a precise instrument high performance liquid chromatography is needed. Compared with the existing method, the reagent and the method provided by the embodiment of the invention are simple, and the method is simple to operate, fast in speed and low in cost.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (8)
1. A reagent for rapidly evaluating the slow release performance of sodium butyrate, comprising: bromothymol blue sodium solution, m-cresol purple solution, phenol red solution and phenolphthalein solution.
2. The reagent for rapidly evaluating sodium butyrate sustained-release performance according to claim 1, wherein the mixing ratio of the bromothymol blue sodium solution, the m-cresol purple solution, the phenol red solution and the phenolphthalein solution is 2-4: 4 to 5:0.01 to 1:1 to 2.
3. The reagent for rapid evaluation of sodium butyrate sustained-release property according to claim 2, wherein the concentration of the bromothymol blue sodium solution, the m-cresol purple solution, the phenol red solution and the phenolphthalein solution is 0.2 to 0.7g/L, 0.8 to 1.3g/L, 0.1 to 0.3g/L and 0.5 to 2.0g/L, respectively.
4. The reagent for rapid evaluation of sodium butyrate sustained release performance according to claim 3, wherein the concentration of the bromothymol blue sodium solution, the m-cresol purple solution, the phenol red solution and the phenolphthalein solution is 0.5g/L, 1.0g/L, 0.2g/L and 1.0g/L, respectively.
5. A method for evaluating sodium butyrate sustained-release performance using the reagent for rapidly evaluating sodium butyrate sustained-release performance according to any one of claims 1 to 4, comprising the steps of:
s1, mixing four solutions of bromothymol blue sodium, m-cresol purple, phenol red and phenolphthalein in proportion, and obtaining a bright orange mixed solution after mixing;
s2, adding 5-8 drops of the mixed solution into water to obtain a light yellow water sample;
s3, adding a sodium butyrate sample into the water sample, and shaking uniformly;
and S4, observing the color change condition of the solution within two hours, and judging the slow release effect of the sodium butyrate sample according to the color change condition of the solution.
6. The method for evaluating a sodium butyrate sustained-release property according to claim 5, wherein in step S1, the mixing ratio of four solutions of bromothymol blue sodium, m-cresol purple, phenol red and phenolphthalein is 2 to 4:4 to 5:0.01 to 1:1 to 2.
7. The method for evaluating a sodium butyrate sustained-release property according to claim 5, wherein in step S3, the effective content of sodium butyrate in the sodium butyrate sample is 1.5-2.5 g.
8. The method for evaluating a slow release performance of sodium butyrate according to claim 5, wherein in step S4, the method for evaluating a slow release effect of a sodium butyrate sample according to a color change condition of a solution specifically comprises:
when the color of the solution changes from yellow to green, then from green to blue and finally from blue to purple, the sodium butyrate sample has a slow release effect;
when the solution is light yellow or light green and has no change, the sodium butyrate sample cannot be released, and the sodium butyrate sample has no slow release effect;
when the solution was blue or purple and there was no change, it was shown that the sodium butyrate sample was completely released and the sodium butyrate sample had no slow release effect.
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