CN117286081B - Microorganism strain combination for promoting growth of elymus chinensis and application - Google Patents
Microorganism strain combination for promoting growth of elymus chinensis and application Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- General Engineering & Computer Science (AREA)
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- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention belongs to the technical field of microorganisms, and particularly relates to a microorganism strain combination for promoting the growth of elymus chinensis and application thereof. Comprises the steps of mixing Sphingomonas aquaticaSphingomonas aquatilis aSzEr 003) and Paenibacillus mucilaginosusPaenibacillus mucilaginosus aSzEe 005) is prepared into a combined microbial inoculum, and the combined microbial inoculum is applied to a preparation for promoting plant growth, so that the germination rate of plants can be effectively improved, the plant height, the root system length and the plant dry weight are increased, and the plant growth is promoted.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a microorganism strain combination for promoting plant growth and application thereof.
Background
The pasture germplasm resource is used as a material basis for sustainable development of grassland animal husbandry in China, and plays an important role in maintaining human survival and national ecological safety. The excellent germplasm resource is an important basis for ensuring resource development and utilization and artificial breeding. Radix Et rhizoma Fagopyri TatariciElymus L.) Is perennial grass of wheat family (Triticum) of Gramineae (Poaceae), which contains anti-tumor agentThe excellent gene for resisting plant diseases and insect pests and adversity stress has the characteristics of drought resistance, cold resistance, pasture resistance, high seed yield and the like, and has higher nutritive value and high feeding value. The grass plant of the genus Oak is early in green turning, can provide feed for grazing livestock early, and has important significance in well developing animal husbandry and maintaining grassland diversity. Therefore, the plant of the genus Oak plays an important role in the restoration of degraded grassland in northwest areas of China, particularly in Qinghai-Tibet plateau, and in the sustainable development of grassland animal husbandry.
The high-quality grass seeds have quite important significance for ecological protection of natural grasslands, efficient development of grassland animal husbandry, construction and planting of artificial grasslands and the like. However, at present, the main bottleneck faced in pasture production and grassland ecological restoration in China is the serious shortage of excellent grass seeds.
In order to promote the germination rate and growth condition of the seeds of the genus Odonia, research ideas of different angles have been developed in recent years. Zhou Juanjuan et al (the effect of oat plant extract on the germination of the seeds of Fangzhuimiao grass and the chemosensory effect of the growth of seedlings; chinese grassland journal, 2021, 8 th) studied the effect of oat plant (stem and leaf, root system) extracts of different concentrations on the germination and growth of the seeds of wild Fangzhuimiao grass, found that oat plant extracts of different concentrations had inhibitory effects on the germination (germination potential, germination rate, germination index, vitality index, germination rate) and the growth of seedlings (seedling length, root length, fresh weight of seedlings, dry weight of roots) of Fangzhuimiao grass seeds. The comprehensive effect of the oat root system leaching solution on the germination of the seeds of the elymus drooping grass is stronger than that of the stem and leaf leaching solution.
Xu Wenbo et al (influence of Chinese sheep Mao Nasheng fungus fermentation broth on seed germination of Eichhornia crassipes, science of grass industry, period 6 of 2022) to isolate Chinese festuca from Gansu, qinghai and SichuanFestuca sinensis) Fermentation liquor of endophytic fungus strain in seeds is used as material, and the method researches the herba Oak Caesalpiniae after treatment of fermentation liquor with different concentrationsElymus nutans) The germination condition of seeds and the growth conditions of radicle and embryo find that the effect of the endophytic fungi fermentation liquid is different along with the change of the concentration of the endophytic fungi fermentation liquid, and the endophytic fungi fermentation liquid has different effects on the germination and growth of non-host elymus dropsy seedsThey are promoting and the strains isolated from different geographical hosts behave differently.
Based on the above, the development of a microbial agent capable of promoting plant growth is continued, and the microbial agent has great significance.
Disclosure of Invention
In view of the above problems, the present invention aims to provide a combined microbial inoculant capable of promoting the growth of the grass, which comprises Sphingomonas aquatica and Paenibacillus mucilaginosus, and can achieve the purpose of promoting the growth of plants by promoting the germination of the plants, promoting the increase of the plants, promoting the development of plant root systems and promoting the absorption of nutrition by the plants.
In order to achieve the above purpose, the present invention may adopt the following technical scheme:
in a first aspect, the invention provides a microbial composition comprising Sphingomonas aquatica and Paenibacillus mucilaginosus.
Wherein Sphingomonas aquatica isSphingomonas aquatilis aSzEr003, the preservation number is CCTCC No. M2023997, and the paenibacillus mucilaginosus isPaenibacillus mucilaginosusaSzEe005 has a preservation number of CCTCC No. M2023998.
Preserving strain information:
sphingomonas aquaticaSphingomonas aquatilis) aSzEr003, preservation mechanism: china Center for Type Culture Collection (CCTCC); address: university of martial arts, martial arts; preservation date: 2023, 6, 12; preservation number: cctccc No. M2023997; classification naming:Sphingomonas aquatilis。
paenibacillus mucilaginosus @Paenibacillus mucilaginosus) aSzEe005, preservation agency: china Center for Type Culture Collection (CCTCC); address: university of martial arts, martial arts; preservation date: 2023, 6 and 12 days, wherein the preservation number is CCTCC No. M2023998; classification naming:Paenibacillus mucilaginosus。
in a second aspect, the present invention provides a product comprising the microbial composition of the first aspect or a fermentation product thereof.
Preferably, the total of the microbial compositions in the productThe viable count is not less than 10 6 CFU/g or 10 6 The ratio of the viable count of CFU/mL, sphingomonas aquatica and Paenibacillus mucilaginosus is 1-2:1-2; for example, the ratio of the viable count of Sphingomonas aquatica to Paenibacillus mucilaginosus is 1:1, 1:1.5, 1:2, 2:1, 2:1.5.
Preferably, the product is any one of seed soaking liquid, bacterial mud, seed coating or fertilizer. Preferably, the fertilizer is a foliar fertilizer.
In a third aspect, the invention provides the use of said microbial composition or said product for promoting germination or growth of plants.
In some embodiments, the above-described applications may include one or more of the following application combinations:
(1) The application in improving the germination rate of plants;
(2) The application in improving the plant height;
(3) Application in promoting plant root system development;
(4) Use in promoting plant nutrient absorption;
(5) Use in promoting weight gain in plants.
Preferably, the plant is a gramineous plant; more preferably, the plant is a plant of the genus Oak. More preferably, the plant is one or more of Fagopyrum tataricum, mesona chinensis, fagopyrum laowii, fagopyrum cylindrical, fagopyrum niruri.
Preferably, the total viable count of the microbial composition is not less than 10 6 CFU/g or 10 6 The ratio of the viable count of CFU/mL, sphingomonas aquatica and Paenibacillus mucilaginosus is 1-2:1-2, for example, the ratio of the viable count of Sphingomonas aquatica and Paenibacillus mucilaginosus is 1:1, 1:1.5, 1:2, 2:1, 2:1.5.
In a fourth aspect, the present invention provides a method for culturing the microorganism composition, comprising the steps of: sphingomonas aquatica and Paenibacillus mucilaginosus are respectively or jointly inoculated into an R2A culture medium and cultured for 18-24 hours at the temperature of 30-37 ℃.
In some embodiments, what isThe formula of the R2A culture medium is as follows: soluble starch 2.5 g, glucose 2.5 g, bacteriological peptone 2.5 g, acid casein 2.5 g, yeast extract 2.5 g, K 2 HPO 3 ·3H 2 O1.5 g, sodium pyruvate 1.5g, mgSO 4 ·7H 2 O0.25 g, distilled water 1000 mL.
In a fifth aspect, the present invention provides a method of treating plant seeds, comprising the steps of:
soaking plant seeds in the seed soaking liquid obtained by fermenting and diluting the microbial composition in the first aspect, and coating the soaked plant seeds with a coating agent prepared by mixing activated carbon and trichoderma harzianum powder.
Preferably, the method for treating plant seeds comprises the following steps:
s1, seed soaking liquid preparation: respectively inoculating Sphingomonas aquatica aSzEr003 and Paenibacillus mucilaginosus aSzEe005 into an R2A culture medium, culturing for 18-24 hours at 30-37 ℃, collecting bacterial cells and extracellular polysaccharide, and centrifuging to obtain bacterial sludge; mixing and diluting the two bacterial sludge to prepare seed soaking liquid;
further preferably, the total viable count of the two bacterial sludge is not less than 10 after mixing 9 CFU/g, the ratio of the viable count of the two bacteria is 1-2:1-2;
more preferably, the total viable count of the two bacterial sludge is 10 after being mixed 9 ~10 11 CFU/g。
S2, seed soaking: soaking plant seeds with the seed soaking liquid, fishing out and draining to obtain soaked plant seeds;
further preferably, the seed soaking time is 10-30min.
S3, preparing a coating agent: mixing active carbon with trichoderma harzianum powder to prepare a coating agent;
preferably, the weight ratio of the activated carbon to the trichoderma harzianum powder is 8-10:1, for example, 8:1, 9:1, 10:1; wherein the total viable count is not less than 10 9 CFU/g。
S4, coating agent is coated: adding the coating agent into the plant seeds after seed soaking, uniformly mixing and airing.
Further preferred, the weight ratio of the seed of the plant after seed soaking to the coating agent is 80-100:1, e.g. 80:1, 85:1, 90:1, 95:1, 100:1; the thickness of the coating agent on the surface of the seeds is 0.5-1mm.
It should be noted that germination rate, plant height, root system, nutrient absorption, plant weight, etc. are terms known in the art and have no other special meaning.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention applies Sphingomonas aquatica and Paenibacillus mucilaginosus together in the treatment of plant seeds, can effectively improve the germination rate of plants, and increase the plant height, root length and plant dry weight, thereby promoting the germination or growth of plants.
2. The combined treatment of the mixed seed soaking of the Sphingomonas aquatica and the Paenibacillus mucilaginosus and the trichoderma harzianum powder coating can further remarkably improve the germination rate, the length of the overground part (plant height) and the length of the underground part (root length) of the plant, and can promote the increase of the dry weight of the overground part and the underground part.
3. The Sphingomonas aquatica and the paenibacillus mucilaginosus are jointly used for plant seed treatment and further combined with coating treatment, so that the nutrition absorption of plants can be effectively promoted, the germination of the plants is further promoted, the germination rate is improved, and the growth and root development of the plants are promoted.
Drawings
FIG. 1 is a comparison of the growth of grass under different seed treatment conditions, four groups from left to right: mixed seed soaking + coating group, mixed seed soaking group, coating group and blank control group.
Detailed Description
The examples are presented for better illustration of the invention, but the invention is not limited to the examples. Those skilled in the art will appreciate that various modifications and adaptations of the embodiments described above are possible in light of the above teachings and are intended to be within the scope of the invention.
The endpoints and any values of the ranges disclosed herein are not limited to the precise range or value, and are understood to encompass values approaching those ranges or values. For numerical ranges, one or more new numerical ranges may be found between the endpoints of each range, between the endpoint of each range and the individual point value, and between the individual point value, in combination with each other, and are to be considered as specifically disclosed herein.
The present invention will be described in detail by examples. It should be understood that the following examples are illustrative only of the present invention and are not intended to limit the present invention.
In the following examples, sphingomonas aquatica isSphingomonas aquatilis aSzEr003 which is automatically collected in the inner Mongolian autonomous area of the four-seed king flag is preserved in China Center for Type Culture Collection (CCTCC), and the preservation number is as follows: cctccc No. M2023997; preservation date: 2023, 6, 12, classification naming:Sphingomonas aquatilis。
paenibacillus mucilaginosus asPaenibacillus mucilaginosusaSzEe005 is collected by oneself in the inner Mongolian autonomous area of the prince's flag, preserved in China Center for Type Culture Collection (CCTCC) in 2023, 6 months and 12 days, the preservation number is CCTCC No. M2023998, and the classification and naming are carried out:Paenibacillus mucilaginosus。
in the following examples, "aerial parts" means the parts of plants where soil grows; "subterranean portion" refers to the portion of a plant that grows into the ground, typically the root of the plant.
1. Experimental procedure
Step 1, preparing planting soil: mixing sand soil and nutrient soil according to a volume ratio of 1:2 for standby, and putting the mixture into a planting container as planting soil.
Step 2, seed preparation: 200 seeds of the elymus tenuifolia seeds with uniform size and full grain are selected and randomly divided into 4 groups of 50 seeds each, and each group is respectively planted in a planting container. The 4 groups are seed soaking + coating group, seed soaking group, coating group and blank control group respectively.
Step 3, seed soaking liquid preparation:
preparing mixed seed soaking liquid: grafting Sphingomonas aquatica aSzEr003 and Paenibacillus mucilaginosus aSzEe005 respectivelyCulturing in an R2A culture medium at 30-37 ℃ for 18-24 hours, collecting bacterial cells and extracellular polysaccharide, and centrifuging to obtain bacterial sludge; mixing the two bacterial sludge, and diluting with sterile physiological saline (0.9%) to obtain seed soaking liquid; the total viable count of the two bacterial sludge is 10 after mixing 11 CFU/g, the ratio of the viable count of the two bacteria is 1:1.
preparation of Sphingomonas aquatica aSzEr003 seed soaking liquid: inoculating Sphingomonas aquatica aSzEr003 into an R2A culture medium, culturing at 30-37 ℃ for 18-24 hours, collecting bacterial cells and extracellular polysaccharide, and centrifuging to obtain bacterial mud; the Sphingomonas aquatica aSzEr003 bacterial mud is diluted by sterile normal saline (0.9%) to prepare Sphingomonas aquatica aSzEr003 seed soaking liquid.
Preparing Paenibacillus mucilaginosus aSzEe005 seed soaking liquid: inoculating Paenibacillus mucilaginosus aSzEe005 into an R2A culture medium, culturing for 18-24 hours at 30-37 ℃, collecting bacterial cells and extracellular polysaccharide, and centrifuging to obtain bacterial sludge; the paenibacillus mucilaginosa aSzEe005 bacterial mud is diluted by sterile physiological saline (0.9 percent) to prepare the paenibacillus mucilaginosa aSzEe005 seed soaking liquid.
The formula of the R2A culture medium is as follows: soluble starch 2.5 g, glucose 2.5 g, bacteriological peptone 2.5 g, acid casein 2.5 g, yeast extract 2.5 g, K 2 HPO 3 ·3H 2 O1.5 g, sodium pyruvate 1.5g, mgSO 4 ·7H 2 O0.25 g, distilled water 1000 mL.
Step 4, preparing a coating agent: mixing active carbon and trichoderma harzianum powder according to the weight ratio of 9:1 to obtain the coating agent, wherein the total viable count is 10 9 CFU/g。
Step 5. Plant seeds were treated in different ways, see in detail the following examples and comparative examples 1-5.
And 6, planting 4 groups of seeds into planting soil, and culturing under the same illumination condition. After growing for 4 days, the germination of each group of seeds was observed.
The germination rate calculation formula: germination rate = number of germinated seeds/total number of seeds x 100%.
And 7, after 15 days of growth, pulling out all plants, cleaning up planting soil adhered to the plants, dividing the plants into overground parts and underground parts, measuring the length of overground and underground parts of each plant by using a graduated scale, and calculating the length average value of overground parts and the length average value of underground parts of each group.
And 8, respectively placing the upper part and the underground part of each group of the elymus chinensis in a low-temperature oven for drying to constant weight, and weighing the total dry weight of the upper part and the total dry weight of the underground part of each group of the elymus chinensis.
Examples
Soaking the seeds of the grass in the mixed seed soaking liquid prepared in the step 3 for 20min, fishing out and draining; and adding a coating agent into the seed of the grass seeds after seed soaking, wherein the weight ratio of the seed of the grass seeds after seed soaking to the coating agent is 90:1, the thickness of the coating agent on the surfaces of the seeds is 0.5-1mm, uniformly mixing and airing.
Comparative example 1
Soaking seeds of the grass seeds for 20min by using the mixed seed soaking liquid prepared in the step 3, fishing out and draining.
Comparative example 2
Coating, soaking the seeds of the grass with sterile water for 20min, and taking out and draining; then adding a coating agent into the seeds of the grass, wherein the weight ratio of the seeds of the grass to the coating agent after seed soaking is 90:1, the thickness of the coating agent on the surfaces of the seeds is 0.5-1mm, uniformly mixing and airing.
Comparative example 3
And (5) in the blank control group, soaking the grass seeds in sterile water for 20min, and taking out and draining.
Comparative example 4
Soaking seeds of the grass seeds in Sphingomonas aquatica aSzEr003 seed soaking liquid for 20min, fishing out and draining; and adding a coating agent into the seed of the grass seeds after seed soaking, wherein the weight ratio of the seed of the grass seeds after seed soaking to the coating agent is 90:1, the thickness of the coating agent on the surfaces of the seeds is 0.5-1mm, uniformly mixing and airing.
Comparative example 5
Soaking seeds of grass seeds in Paenibacillus mucilaginosus aSzEe005 seed soaking solution for 20min, taking out and draining; and adding a coating agent into the seed of the grass seeds after seed soaking, wherein the weight ratio of the seed of the grass seeds after seed soaking to the coating agent is 90:1, the thickness of the coating agent on the surfaces of the seeds is 0.5-1mm, uniformly mixing and airing.
2. Experimental results
The results of the related experiments are shown in Table 1.
TABLE 1
Note that: different lower case letters indicate that there is a significant difference in the columns (P < 0.05)
From the experimental results, it can be seen that the combined treatment of mixed seed soaking and coating can significantly improve the germination rate, the length of the overground part (plant height) and the length of the underground part (root length) of the conga, and can promote the increase of the dry weight of the overground part and the underground part.
Compared with the single strain seed soaking liquid treatment, the germination rate of the mixed seed soaking liquid is obviously increased by 19.4-21.6%; the overground length of the plant is obviously increased by 10.1 to 14.0 percent, and the underground length is obviously increased by 7.9 to 9.1 percent; the dry weight of the plant on the ground is increased by 8.5-15.9%, and the dry weight of the underground part is increased by 9.5-11.4% (P < 0.05).
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (9)
1. A microbial composition, characterized in that the microbial composition is Sphingomonas aquatica and Paenibacillus mucilaginosus;
the Sphingomonas aquatica is Sphingomonas aquatilis aSzEr, classified and named Sphingomonas aquatilis, and is preserved in China Center for Type Culture Collection (CCTCC) No. M2023997, and the preservation date is 2023, 6 months and 12 days;
the Paenibacillus mucilaginosus is Paenibacillus mucilaginosus aSzEe005, classified and named Paenibacillus mucilaginosus, and is preserved in China Center for Type Culture Collection (CCTCC) No. M2023998, and the preservation date is 2023, 6 and 12 days.
2. A product comprising the microbial composition of claim 1 or a fermentation thereof; the product is any one of seed soaking liquid, bacterial mud, seed coating or fertilizer.
3. The product according to claim 2, wherein the total viable count of the microbial composition in the product is not less than 10 6 CFU/g or 10 6 The ratio of the viable count of CFU/mL, sphingomonas aquatica and Paenibacillus mucilaginosus is 1-2:1-2.
4. Use of a microbial composition according to claim 1 or a product according to claim 2 for promoting germination or growth of plants of the genus elymus.
5. The use according to claim 4, wherein the use is one or more of,
(1) The application in improving the germination rate of plants;
(2) The application in improving the plant height;
(3) Application in promoting plant root system development;
(4) Use in promoting weight gain in plants.
6. The use according to claim 4, wherein the total viable count of the microbial composition is not less than 10 6 CFU/g or 10 6 The ratio of the viable count of CFU/mL, sphingomonas aquatica and Paenibacillus mucilaginosus is 1-2:1-2.
7. A method of treating plant seeds, the method comprising the steps of:
soaking plant seeds in the seed soaking liquid prepared by fermenting and diluting the microbial composition according to claim 1, and coating the soaked plant seeds with a coating agent prepared by mixing activated carbon and trichoderma harzianum powder; the plant is a plant of the genus Eleutherococcus.
8. The processing method according to claim 7, characterized in that the processing method comprises the steps of:
s1, seed soaking liquid preparation: respectively inoculating Sphingomonas aquatica Sphingomonas aquatilis aSzEr003 and Paenibacillus mucilaginosus Paenibacillus mucilaginosus aSzEe005 into an R2A culture medium, culturing at 30-37 ℃ for 18-24 hours, collecting bacterial cells and extracellular polysaccharide, and centrifuging to obtain bacterial mud; mixing and diluting the two bacterial sludge to prepare seed soaking liquid;
s2, seed soaking: soaking plant seeds with the seed soaking liquid, fishing out and draining to obtain soaked plant seeds;
s3, preparing a coating agent: mixing active carbon with trichoderma harzianum powder to prepare a coating agent;
s4, coating agent is coated: adding the coating agent into the plant seeds after seed soaking, uniformly mixing and airing.
9. The process of claim 8, wherein,
in the step S1, the total viable count of the two bacterial sludge is not less than 10 after being mixed 9 The ratio of the viable count of CFU/g, sphingomonas aquatica and Paenibacillus mucilaginosus is 1-2:1-2;
in the step S2, the seed soaking time is 10-30min;
in the step S3, the weight ratio of the active carbon to the trichoderma harzianum powder is 8-10:1, and the total viable count in the coating agent is not less than 10 9 CFU/g；
In the step S4, the weight ratio of the plant seeds after seed soaking to the coating agent is 80-100:1, and the thickness of the coating agent on the surfaces of the seeds is 0.5-1mm.
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| CN111387209A (en) * | 2020-05-21 | 2020-07-10 | 湖北大学 | Composite antagonistic bacterium agent for preventing and controlling tobacco diseases and application thereof |
| CN114717141A (en) * | 2022-03-04 | 2022-07-08 | 浙江台州秀川科技有限公司 | Complex flora for treating wastewater containing gallic acid and application thereof |
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| CN111387209A (en) * | 2020-05-21 | 2020-07-10 | 湖北大学 | Composite antagonistic bacterium agent for preventing and controlling tobacco diseases and application thereof |
| CN114717141A (en) * | 2022-03-04 | 2022-07-08 | 浙江台州秀川科技有限公司 | Complex flora for treating wastewater containing gallic acid and application thereof |
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