CN117233056A - Biological aerosol aerodynamic spectrometer - Google Patents
Biological aerosol aerodynamic spectrometer Download PDFInfo
- Publication number
- CN117233056A CN117233056A CN202311511721.4A CN202311511721A CN117233056A CN 117233056 A CN117233056 A CN 117233056A CN 202311511721 A CN202311511721 A CN 202311511721A CN 117233056 A CN117233056 A CN 117233056A
- Authority
- CN
- China
- Prior art keywords
- light
- aerodynamic
- laser
- aerosol
- spectrometer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000443 aerosol Substances 0.000 title claims abstract description 77
- 239000002245 particle Substances 0.000 claims abstract description 86
- 238000001514 detection method Methods 0.000 claims abstract description 29
- 239000013078 crystal Substances 0.000 claims abstract description 21
- 230000005284 excitation Effects 0.000 claims abstract description 16
- 238000002347 injection Methods 0.000 claims abstract description 8
- 239000007924 injection Substances 0.000 claims abstract description 8
- 230000003287 optical effect Effects 0.000 claims description 14
- 230000010287 polarization Effects 0.000 claims description 5
- 239000004065 semiconductor Substances 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 238000005070 sampling Methods 0.000 claims 2
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000001917 fluorescence detection Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 229910021532 Calcite Inorganic materials 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
Abstract
The invention discloses a biological aerosol aerodynamic spectrometer, which comprises an aerosol sample injection module, an excitation laser light path module and a light collecting module, wherein an aerodynamic lens is adopted, so that the diameter and the divergence angle of a focused aerosol particle beam are reduced, a false signal of the back flow of the aerosol particles generated by the fact that the aerosol particles float outside a lower air nozzle due to the fact that the diameter and the divergence angle of the aerosol particle beam are large is reduced, in addition, the size of an excitation laser spot can be further reduced due to the fact that the diameter of the aerosol particle beam is small, the energy density of the spot is improved, the fluorescent signal intensity of an instrument is improved, the collection efficiency of fluorescent signals is improved by adopting a semi-ellipsoidal reflector, and the energy ratio of two mutually parallel elliptic spots is further improved by adopting a wave plate and a cylindrical focusing lens to be matched with a birefringent crystal, so that the intensity of the fluorescent signals collected by the instrument is further improved, and the detection sensitivity of the biological aerosol particle detector can be further improved.
Description
Technical Field
The invention relates to the technical field of aerosol detection in air, in particular to a biological aerosol aerodynamic spectrometer.
Background
In recent years, the spread of bacterial and viral infections in animals and humans has resulted in significant losses in the development of society and economy. In particular, the pandemic of some viruses has gradually increased the emphasis on biological security.
In the transmission pathway of biologically pathogenic substances. Aerosol transmission is one of the most widely spread and most harmful transmission routes for biological pathogenic substances. Bioaerosols refer to biological particles suspended in air that enter the human body, usually through the respiratory system of the human body, causing various diseases. At present, a laser-induced bio-fluorescence detection method is often adopted to detect the concentration of the bioaerosol in the air.
The structure of the conventional common bioaerosol particle detector adopts a single nozzle or a nozzle with sheath air flow as a sample injection nozzle, and the single nozzle structure causes larger emergent air flow divergence, so that particle beams of the air flow are large, and larger excitation laser spots are needed to cover the whole particle air flow beam, thereby reducing the energy density of the excitation laser in unit area. The intrinsic fluorescence intensity of the particles is positively correlated with the energy density per unit area of the excitation laser, thus reducing the fluorescence detection intensity. In addition, the single nozzle structure is easy to generate particle backflow due to larger divergence, and a plurality of interference pseudo particle signals are generated. In the fluorescence collection optical path system, a spherical mirror and lens scheme is generally adopted, and fluorescence and scattered light are detected in a distinguishing mode through a dichroic mirror. However, since the numerical aperture of spherical mirrors and lenses is limited, the light collection efficiency is generally not high. These two aspects cause the defect of the detection sensitivity of the current bioaerosol particle detector, and early or low-activity bioaerosol particles in the air are difficult to detect, so we provide a bioaerosol aerodynamic spectrometer.
Disclosure of Invention
It is an object of the present invention to provide a bioaerosol aerodynamic spectrometer that solves or at least alleviates one or more of the above-mentioned problems and other problems with the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions: a bioaerosol aerodynamic spectrometer comprising:
the aerosol sample injection module is used for drawing aerosol particles in the air into aerosol particle beams with required diameters and divergence angles and then sending the aerosol particle beams into the detection cavity;
the excitation laser light path module is used for coupling laser emitted by the laser into two mutually parallel elliptical light spots and then emitting the two mutually parallel elliptical light spots into the detection cavity, and aerosol particles entering the detection cavity are irradiated by the two mutually parallel elliptical light spots to generate scattered light and fluorescence;
and the light collecting module is used for respectively receiving the generated scattered light and the generated fluorescence.
In the bioaerosol aerodynamic spectrometer according to the present invention, optionally, the light collecting module includes a reflecting mirror, the reflecting mirror is arranged in a semi-ellipsoidal shape with an opening at one side, the reflecting mirror is fixedly installed inside the detection cavity, a fluorescence receiving unit for collecting fluorescence is disposed in an opening direction of the reflecting mirror, and a scattered light receiving unit for receiving scattered light is disposed at a side of the reflecting mirror away from the opening direction thereof.
In the bioaerosol aerodynamic spectrometer according to the present invention, optionally, the fluorescence receiving unit includes an optical filter and a first photodetector, the optical filter is configured to filter the focused light collected by the reflecting mirror, reflect or absorb the scattered light signal therein, and only collect the fluorescence signal therein onto the first photodetector, where the first photodetector is configured to convert the fluorescence signal into an electrical signal.
In the bioaerosol aerodynamic spectrometer according to the present invention, optionally, the scattered light receiving unit includes a focusing lens disposed near a long axis vertex of the reflecting mirror, and a second photodetector for converting the scattered light signal into an electrical signal, the focusing lens being for collecting and focusing the scattered light onto the second photodetector.
In the bioaerosol aerodynamic spectrometer according to the present invention, optionally, the excitation laser light path module includes the laser, a wave plate, a cylindrical focusing lens, and a birefringent crystal;
the laser is a continuous semiconductor laser, and the laser is arranged outside one side of the detection cavity;
the wave plate is arranged on a light path of laser emitted by the laser and is used for adjusting the polarization state of an incident light spot;
the cylindrical focusing lens is arranged on one side of the wave plate, which is far away from the laser, and is used for focusing a collimation light spot emitted by the laser to generate an elliptic light spot;
the double refraction crystal is arranged on one side of the cylindrical focusing lens, which is far away from the wave plate, and the double refraction crystal refracts the elliptical light spots into two parallel elliptical light spots.
In the bioaerosol aerodynamic spectrometer according to the invention, optionally, the major axis direction of the elliptical spot is perpendicular to the aerosol particle beam direction entering the detection cavity, and the diameter of the elliptical spot is larger than the diameter of the aerosol particle beam.
In the bioaerosol aerodynamic spectrometer according to the invention, optionally, an optical trap is arranged on a side of the detection cavity away from the laser, and the optical trap is used for reflecting or absorbing the excitation laser light which is emitted out of the reflecting mirror, so as to prevent the laser light from reentering the detection cavity to form noise signals.
In the bioaerosol aerodynamic spectrometer according to the present invention, optionally, the aerosol sample injection module includes an aerodynamic lens, the aerodynamic lens includes a hollow cylindrical long barrel, the top and the bottom of the cylindrical long barrel are both provided with openings, a plurality of annular stop lenses are fixedly installed inside the cylindrical long barrel from top to bottom in sequence, the aperture of the annular stop lenses is gradually reduced from top to bottom, and an air outlet at the lower end of the aerodynamic lens extends into the reflector.
In the bioaerosol aerodynamic spectrometer according to the present invention, optionally, the aerosol sample injection module further comprises a lower air tap, an air inlet at the upper end of the lower air tap extends into the reflector, and an air outlet of the lower air tap is connected with a vacuum system.
In the bioaerosol aerodynamic spectrometer according to the invention, optionally, the air inlet of the lower air tap is located directly below the air outlet of the aerodynamic lens
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the diameter and the divergence angle of a focused aerosol particle beam are reduced by adopting the aerodynamic lens, so that the false signal of the back flow of the aerosol particles generated by the fact that the aerosol particles float outside the lower air tap due to the fact that the diameter and the divergence angle of the aerosol particle beam are large is reduced, in addition, the size of an excitation laser spot can be further reduced due to the fact that the diameter of the aerosol particle beam is small, the energy density of the spot is improved, the fluorescence signal intensity of an instrument is improved, the collection efficiency of the fluorescence signal is improved by adopting a semi-ellipsoidal reflector, the energy ratio of two mutually parallel elliptical spots is adjusted by adopting a wave plate and a cylindrical focusing lens in combination with a birefringent crystal, the energy of a single spot is further improved, the fluorescence signal intensity collected by the instrument is further improved, and the detection sensitivity of the biological aerosol particle detector can be further effectively improved.
Drawings
FIG. 1 is a schematic cross-sectional elevation view of a bioaerosol aerodynamic spectrometer of the present invention;
FIG. 2 is a schematic top view cross-sectional structure of a bioaerosol aerodynamic spectrometer of the present invention;
FIG. 3 is a schematic diagram of the aerodynamic lens structure of the bioaerosol aerodynamic spectrometer of the present invention;
FIG. 4 is a schematic diagram of the structure of two mutually parallel elliptical spots in a bioaerosol aerodynamic spectrometer of the present invention;
FIG. 5 is a schematic pulse diagram of scattered light and fluorescent signals in a bioaerosol aerodynamic spectrometer of the present invention;
figure 6 is a graph of particle diameter statistics for an embodiment of a bioaerosol aerodynamic spectrometer of the present invention.
In the figure: 101. a laser; 102. a wave plate; 103. a cylindrical focusing lens; 104. a birefringent crystal; 105. an optical trap;
201. an aerodynamic lens; 202. an annular stop lens; 203. a lower air tap;
301. a reflecting mirror; 302. a focusing lens; 303. a second photodetector; 304. a light filter; 305. a first photodetector;
4. aerosol particles; 5. and a detection cavity.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Referring to fig. 1 to 6, the present invention provides a technical solution:
the biological aerosol aerodynamic spectrometer comprises an aerosol sample injection module, a light collection module and an excitation laser light path module.
In this embodiment, the light collecting module for receiving the generated scattered light and fluorescence respectively includes a reflecting mirror 301, the reflecting mirror 301 is arranged in a semi-ellipsoidal shape with an opening at one side, the reflecting mirror 301 is fixedly installed inside the detecting cavity 5, a fluorescence receiving unit for collecting the fluorescence is arranged in the opening direction of the reflecting mirror 301, a scattered light receiving unit for receiving the scattered light is arranged at one side of the reflecting mirror 301 far away from the opening direction, the fluorescence receiving unit includes a light filter 304 and a first photodetector 305, the light filter 304 is used for filtering the light focused by the reflecting mirror 301, reflecting or absorbing the scattered light signal therein, only the fluorescent signal therein is remained and collected on the first photodetector 305, the first photodetector 305 is used for converting the fluorescent signal into an electrical signal, typically PMT, the scattered light receiving unit includes a focusing lens 302 and a second photodetector 303, the focusing lens 302 is arranged at the top point close to the opening direction of the reflecting mirror 301, the focusing lens 302 is used for collecting the scattered light and focusing the scattered light onto the second photodetector 303, and the second photodetector is used for converting the scattered light signal into the electrical signal typically into the APD signal or PMT.
In this example, the excitation laser light path module includes a laser 101, a wave plate 102, a cylindrical focusing lens 103, and a birefringent crystal 104;
wherein, the laser 101 adopts a 405nm wave continuous semiconductor laser which corresponds to the absorption peak of (NAD (P) H) in biological bacteria and can continuously excite the intrinsic fluorescent signal in the living beings, and the laser 101 is arranged outside one side of the detection cavity 5;
the cylindrical focusing lens 103 is arranged at one side of the wave plate 102 far away from the laser 101, the cylindrical focusing lens 103 is used for focusing a collimation light spot emitted by the laser 101 to generate an elliptic light spot, wherein the cylindrical focusing lens 103 can be a cylindrical mirror or a cylindrical mirror group, the long axis direction of the elliptic light spot is perpendicular to the direction of an aerosol particle beam entering the detection cavity 5, and the diameter of the elliptic light spot is larger than the diameter of the aerosol particle beam so as to ensure that aerosol particles 4 on the aerosol particle beam can be effectively excited by laser;
the birefringent crystal 104 is disposed on a side of the cylindrical focusing lens 103 away from the wave plate 102, and the birefringent crystal 104 is typically a calcite crystal, and because of its birefringent effect, the birefringent crystal 104 is capable of refracting an elliptical light spot into two parallel elliptical light spots, the offset distance of which is related to the angle and thickness of the birefringent crystal 104. The energy ratio of the two elliptical light spots is related to the polarization state of the incident light spot;
the wave plate 102 is arranged on the light path of the laser light emitted by the laser 101, and the wave plate 102 is used for adjusting the polarization state of the incident light spot, so as to adjust the energy ratio of the two elliptical light spots after the laser light passes through the birefringent crystal 104, and a 1/2 wave plate or a 1/4 wave plate can be adopted.
In order to reduce noise signals, an optical trap 105 is disposed on a side of the detection cavity 5 away from the laser 101, and the optical trap 105 is configured to reflect or absorb the excitation laser light that will exit the mirror 301, so as to avoid that the laser light reenters the detection cavity 5 to form noise signals.
In this example, the aerosol sample injection module for drawing the aerosol particles 4 in the air into the aerosol particle beam with the required diameter and divergence angle and then sending the aerosol particle beam into the detection cavity 5 comprises an aerodynamic lens 201, the aerodynamic lens 201 comprises a cylindrical long barrel which is arranged in a hollow mode, the top and the bottom of the cylindrical long barrel are all arranged in an opening mode, a plurality of annular stop lenses 202 are fixedly installed inside the cylindrical long barrel from top to bottom in sequence, the aperture of the annular stop lenses 202 from top to bottom is gradually reduced, when the aerosol particles 4 pass through the aerodynamic lens 201, when the aerosol particles 4 pass through one annular stop lens 202, the airflow can generate a drag force towards the center due to the resistance of the annular stop lens 202 and the pressure difference between the upper and lower parts of the annular stop lens 202, and the aerosol particle beam is pulled to move towards the center of the lens, so that the diameter and the divergence angle of the aerosol particle beam are gradually reduced, after the aerosol particle beam is converged through the multistage lenses, the lower end air outlet of the aerodynamic lens 201 enters the inside of the reflector 301, the air inlet 203 at the upper end of the lower air inlet 203 stretches into the inside the reflector 301, the air inlet 203 at the upper end of the lower air inlet 203 is connected with the air inlet 203, the air inlet 203 is connected with the air inlet 203 at the lower end of the lower air inlet 203, and the air inlet 203 can be stably sprayed out of the air inlet 201 and can be stably under the vacuum condition, and the vacuum condition is stable, and the air particle can be detected, and the vacuum particles can be blown out from the air inlet 201 is stable, and can pass through the vacuum system, and the vacuum particle can be stably, and the vacuum particles can be blown out by the air inlet 201.
Example 2
This embodiment differs from embodiment 1 in that:
the aerodynamic lens 201 of the example employs four stages of lenses, the aerodynamic lens 201 having an intra-cavity diameter of 10mm, the aperture of each stage of annular stop lens 202 being as follows:
| first stage | Second stage | Third stage | Air outlet | |
| Diameter mm | 2.72 | 2.32 | 1.95 | 1.20 |
The effective focusing of 0.3-10um can be realized, the aerosol particles 4 are about 0.6mm at the position of 1.5mm below the lens, compared with the common lens, the aerosol particles are smaller in divergence angle, and the problem that the edge particles cannot enter the lower air tap and rotate in the vacuum cavity is reduced. The swirling aerosol particles 4 re-enter the lower nozzle 203.
The laser focusing light spot is designed to be 0.9mm and larger than the diameter of the aerosol particle beam. The laser beam splitting distance is determined by the design of the birefringent crystal 104, and is determined by the incidence plane of the birefringent crystal 104, the birefringence difference, and the lens thickness. The birefringent crystal 104 is here set to 0.35mm. Because different particle sizes have a one-to-one correspondence with the flying speed after passing through the lens, when the aerosol particles 4 penetrate through two elliptical light spots, the scattered light PMT can generate two light signals, so that the flying time of the scattered light PMT is calculated, and then the aerodynamic diameter of the aerosol particles 4 is calculated. The velocity of the returned aerosol particles 4 generated by the common nozzle is much lower than that of the aerosol particles 4 directly coming out, and a signal packet with larger pulse width and wider pulse width is generated, so that the acquisition of the signals of the normal aerosol particles 4 can be influenced. And aerosol particles 4 may form multiple convolutions within mirror 301, interfering with the counting of particles. Particles were counted over time as shown in figure 6.
As the beam width of the particle beam is reduced, the laser light spot can be reduced, so that the energy density of laser is improved, the signal to noise ratio for fluorescence collection is improved, and the energy density is 2-3 times that of a fluorescence counter which generally adopts a common nozzle.
In order to facilitate understanding of the above technical solutions of the present invention, the following describes in detail the working principle or operation manner of the present invention in the actual process.
Working principle: the lower air tap 203 of the bioaerosol particle detector is connected with a vacuum system to provide a stable vacuum pressure difference, and aerosol particles 4 enter the reflecting mirror 301 from the air inlet of the aerodynamic lens 201 and form aerosol particle beams through the aerodynamic lens 201. Wherein the aerosol particle beam is focused into a finer, less divergent aerosol particle beam per pass through the primary annular stop lens 202. The aerosol particle beam exits from the air outlet of the aerodynamic lens 201, enters the detection cavity 5, then enters the lower air tap 203 opposite to the detection cavity, and is discharged through the channel of the lower air tap 203. Compared with a common nozzle, the nozzle with the aerodynamic lens 201 has smaller aerosol particle beam width and smaller divergence angle, and reduces the backflow phenomenon of the aerosol particles 4.
The aerosol particles 4 entering the detection cavity 5 are irradiated by the two parallel elliptical light spot lasers, and scattered light and fluorescence are generated. The long axis of the elliptical light spot is designed to be slightly larger than the diameter of the particle aerosol particle beam, and the aerosol particle beam formed by the aerodynamic lens 201 is smaller than the diameter and the divergence angle of the aerosol particle beam of a common single nozzle, so that the elliptical light spot is designed to be smaller to improve the energy density of the light spot in unit area so as to improve the scattered light and the fluorescence intensity.
And the scattered light is 3-4 orders of magnitude higher than the fluorescence intensity, so that the invention adopts different collecting methods. For scattered light, since the intensity of the scattered light is high, a focusing lens 302 is used as a collecting and focusing optical path and is converged into a second photodetector 303 to generate a scattered light signal pulse. Since the excitation laser is shaped into two parallel elliptical light spots with a certain interval by the cylindrical focusing lens 103 and the birefringent crystal 104, the signal obtained in the second photodetector 303 is two adjacent signal pulses, the time difference between the two adjacent pulses is the flight time of the aerosol particles 4 passing through the two elliptical light spots, and the flight speed of the aerosol particles 4 can be obtained by dividing the interval distance between the two elliptical light spots by the flight time. According to the design relation of the aerodynamic lens, the aerodynamic diameter of the aerosol particles 4 can be obtained through conversion, and the aerodynamic diameter can be used as a criterion of biological particles and non-biological particles.
The laser excited fluorescent signals are collected and focused by the reflector 301, and the scattered light signals are reflected or absorbed by the optical filter 304, so that only the fluorescent signals remain and are converged into the first photodetector 305. The semi-ellipsoidal mirror 301 has a higher collection efficiency than the spherical mirror and lens combination, thereby further improving the fluorescence signal intensity. By adjusting the waveplate 102 in the laser path module, the polarization state of the laser light can be adjusted, thereby adjusting the intensity ratio of the two elliptical spots produced by the birefringent crystal 104. The laser energy ratio can be adjusted to two elliptical spots, one stronger above and one weaker below, for example 70:30, so that the energy of the first laser beam is as large as possible to generate more fluorescent signals.
Since the scattered light is 3-4 orders of magnitude weaker than the fluorescence light, even the scattered light generated in the second laser beam can be effectively detected, and therefore the scattered light signal can be used for detecting the flight time of the particles, and the aerodynamic diameter of the particles can be detected. Because the fluorescence signal is weaker, the first laser beam is improved as much as possible, so that the intensity of the fluorescence signal generated by the first laser beam is improved, and the sensitivity of fluorescence detection is ensured.
None of the inventions are related to the same or are capable of being practiced in the prior art. Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A bioaerosol aerodynamic spectrometer, comprising:
the aerosol sample injection module is used for drawing aerosol particles (4) in the air into aerosol particle beams with required diameters and divergence angles and then sending the aerosol particle beams into the detection cavity (5);
the excitation laser light path module is used for coupling laser emitted by the laser (101) into two mutually parallel elliptical light spots and then emitting the two mutually parallel elliptical light spots into the detection cavity (5), and aerosol particles (4) entering the detection cavity (5) can be irradiated by the two mutually parallel elliptical light spots to generate scattered light and fluorescence;
and the light collecting module is used for respectively receiving the generated scattered light and the generated fluorescence.
2. The bioaerosol aerodynamic spectrometer of claim 1, wherein: the light collecting module comprises a reflecting mirror (301), wherein the reflecting mirror (301) is arranged in a semi-ellipsoidal shape with one side being opened, the reflecting mirror (301) is fixedly installed inside the detection cavity (5), a fluorescent light receiving unit used for collecting fluorescent light is arranged in the opening direction of the reflecting mirror (301), and a scattered light receiving unit used for receiving scattered light is arranged on one side, far away from the opening direction, of the reflecting mirror (301).
3. The bioaerosol aerodynamic spectrometer of claim 2, wherein: the fluorescence receiving unit comprises an optical filter (304) and a first photoelectric detector (305), the optical filter (304) is used for filtering focused light collected by the reflecting mirror (301), scattered light signals in the light are reflected or absorbed, only fluorescence signals in the light are reserved and converged on the first photoelectric detector (305), and the first photoelectric detector (305) is used for converting the fluorescence signals into electric signals.
4. A bioaerosol aerodynamic spectrometer according to claim 3, characterized in that: the scattered light receiving unit comprises a focusing lens (302) and a second photoelectric detector (303), wherein the focusing lens (302) is arranged near the long axis vertex of the reflecting mirror (301), the focusing lens (302) is used for collecting scattered light and focusing the scattered light on the second photoelectric detector (303), and the second photoelectric detector (303) is used for converting scattered light signals into electric signals.
5. The bioaerosol aerodynamic spectrometer of claim 2, wherein: the excitation laser light path module comprises the laser (101), a wave plate (102), a cylindrical focusing lens (103) and a birefringent crystal (104);
wherein the laser (101) is a continuous semiconductor laser, and the laser (101) is arranged outside one side of the detection cavity (5);
the wave plate (102) is arranged on a light path of laser emitted by the laser (101), and the wave plate (102) is used for adjusting the polarization of an incident light spot;
the cylindrical focusing lens (103) is arranged on one side of the wave plate (102) far away from the laser (101), and the cylindrical focusing lens (103) is used for focusing a collimation light spot emitted by the laser (101) to generate an elliptic light spot;
the birefringent crystal (104) is arranged on one side of the cylindrical focusing lens (103) away from the wave plate (102), and the birefringent crystal (104) refracts the elliptical light spot into two elliptical light spots which are parallel to each other.
6. The bioaerosol aerodynamic spectrometer of claim 5, wherein: the long axis direction of the elliptic light spot is perpendicular to the direction of the aerosol particle beam entering the detection cavity (5), and the diameter of the elliptic light spot is larger than that of the aerosol particle beam.
7. The bioaerosol aerodynamic spectrometer of claim 5, wherein: one side of the detection cavity (5) far away from the laser (101) is provided with an optical trap (105), and the optical trap (105) is used for reflecting or absorbing excitation laser light emitted out of the reflecting mirror (301) so as to prevent the laser light from reentering the detection cavity (5) to form noise signals.
8. The bioaerosol aerodynamic spectrometer of claim 7, wherein: the aerosol sampling module comprises an aerodynamic lens (201), the aerodynamic lens (201) comprises a cylindrical long barrel which is arranged in a hollow mode, the top and the bottom of the cylindrical long barrel are both provided with openings, a plurality of annular stop lenses (202) are fixedly installed inside the cylindrical long barrel from top to bottom in sequence, the aperture of each annular stop lens (202) is gradually reduced from top to bottom, and an air outlet at the lower end of the aerodynamic lens (201) stretches into the reflector (301).
9. The bioaerosol aerodynamic spectrometer of claim 8, wherein: the aerosol sampling module further comprises a lower air nozzle (203), an air inlet at the upper end of the lower air nozzle (203) stretches into the reflecting mirror (301), and an air outlet of the lower air nozzle (203) is connected with a vacuum system.
10. The bioaerosol aerodynamic spectrometer of claim 9, wherein: the air inlet of the lower air nozzle (203) is positioned right below the air outlet of the aerodynamic lens (201).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311511721.4A CN117233056B (en) | 2023-11-14 | 2023-11-14 | Biological aerosol aerodynamic spectrometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311511721.4A CN117233056B (en) | 2023-11-14 | 2023-11-14 | Biological aerosol aerodynamic spectrometer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117233056A true CN117233056A (en) | 2023-12-15 |
| CN117233056B CN117233056B (en) | 2024-02-27 |
Family
ID=89089776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311511721.4A Active CN117233056B (en) | 2023-11-14 | 2023-11-14 | Biological aerosol aerodynamic spectrometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117233056B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201107254Y (en) * | 2007-09-26 | 2008-08-27 | 中国人民解放军军事医学科学院微生物流行病研究所 | Ultraviolet laser energy monitoring and excited fluorescent compensating system in aerosol granule analyzer |
| US20080204746A1 (en) * | 2005-11-18 | 2008-08-28 | Gonzalez Cruz Jorge E | Novel optical sensor for the instantaneous detection and identification of bioaerosols |
| CN102116729A (en) * | 2010-01-06 | 2011-07-06 | 北京汇丰隆生物科技发展有限公司 | Light path system for continuous laser particle analyzer |
| CN105403489A (en) * | 2015-12-17 | 2016-03-16 | 中国科学院合肥物质科学研究院 | Apparatus for measuring particle size and shape of atmospheric particulate matters |
| CN106769704A (en) * | 2015-11-20 | 2017-05-31 | 北京汇丰隆经济技术开发有限公司 | A kind of bioaerosol particle optics detection means |
| CN108287129A (en) * | 2018-03-22 | 2018-07-17 | 中国计量大学 | The detection device of multichannel fluorescence Spectra bioaerosol particle |
| CN109196330A (en) * | 2016-05-02 | 2019-01-11 | 汉米尔顿联合股份有限公司 | Real-time optical method and system for biology and abiotic particle to be detected and classified |
-
2023
- 2023-11-14 CN CN202311511721.4A patent/CN117233056B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080204746A1 (en) * | 2005-11-18 | 2008-08-28 | Gonzalez Cruz Jorge E | Novel optical sensor for the instantaneous detection and identification of bioaerosols |
| CN201107254Y (en) * | 2007-09-26 | 2008-08-27 | 中国人民解放军军事医学科学院微生物流行病研究所 | Ultraviolet laser energy monitoring and excited fluorescent compensating system in aerosol granule analyzer |
| CN102116729A (en) * | 2010-01-06 | 2011-07-06 | 北京汇丰隆生物科技发展有限公司 | Light path system for continuous laser particle analyzer |
| CN106769704A (en) * | 2015-11-20 | 2017-05-31 | 北京汇丰隆经济技术开发有限公司 | A kind of bioaerosol particle optics detection means |
| CN105403489A (en) * | 2015-12-17 | 2016-03-16 | 中国科学院合肥物质科学研究院 | Apparatus for measuring particle size and shape of atmospheric particulate matters |
| CN109196330A (en) * | 2016-05-02 | 2019-01-11 | 汉米尔顿联合股份有限公司 | Real-time optical method and system for biology and abiotic particle to be detected and classified |
| CN108287129A (en) * | 2018-03-22 | 2018-07-17 | 中国计量大学 | The detection device of multichannel fluorescence Spectra bioaerosol particle |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117233056B (en) | 2024-02-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2811338T3 (en) | Real-time aerosol monitor | |
| EP2380001B1 (en) | Compact detector for simultaneous particle size and fluorescence detection | |
| EP2694668A1 (en) | Microbial detection apparatus and method | |
| CN102608072A (en) | Microparticle detection apparatus | |
| KR20120071453A (en) | Apparatus for detection of microorganism | |
| KR101878094B1 (en) | Heterogeneous mirror coupled detection apparatus for micro dust and organism | |
| CN208206768U (en) | A kind of optical system | |
| KR101919103B1 (en) | MIRRIRLESS OPTICAL DETECTION APPARATUS of MICROORGANISM | |
| JP7003258B2 (en) | Particle detector | |
| KR102258809B1 (en) | Detection apparatus for micro dust and organism | |
| CN117233056B (en) | Biological aerosol aerodynamic spectrometer | |
| CN109870394A (en) | A kind of dual-beam aerosol particle concentration detection device | |
| KR101825161B1 (en) | Detection apparatus for micro dust and organism | |
| KR20160103291A (en) | Detection apparatus for micro dust and organism | |
| KR102258807B1 (en) | Detection apparatus for micro dust and organism | |
| CN112730180B (en) | High-sensitivity dust particle counting sensor with double detectors | |
| US20220373477A1 (en) | Apparatus for detecting fine dust and microorganisms | |
| CN211856322U (en) | Fluorescence is collected and fluorescence separation structure | |
| CN215414896U (en) | Aerosol particle detection optical system | |
| JP2003004625A (en) | Flow sight meter | |
| CN218445045U (en) | Optical path system of particle counting sensor | |
| CN111024592A (en) | Flow cytometer optical device | |
| CN214794332U (en) | Portable bioaerosol monitoring device | |
| CN219496065U (en) | Novel particle counter light path system | |
| CN217638562U (en) | Particle aerodynamic particle size detection optical-mechanical structure based on right-angle prism |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |