CN117230259B - Method for detecting gas quantity of gas cylinder of three-gas incubator and automatically switching gas cylinders - Google Patents
Method for detecting gas quantity of gas cylinder of three-gas incubator and automatically switching gas cylinders Download PDFInfo
- Publication number
- CN117230259B CN117230259B CN202311520273.4A CN202311520273A CN117230259B CN 117230259 B CN117230259 B CN 117230259B CN 202311520273 A CN202311520273 A CN 202311520273A CN 117230259 B CN117230259 B CN 117230259B
- Authority
- CN
- China
- Prior art keywords
- gas
- cylinder
- gas cylinder
- concentration
- cylinders
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 70
- 239000007789 gas Substances 0.000 claims abstract description 547
- 239000001301 oxygen Substances 0.000 claims abstract description 66
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 66
- 230000007812 deficiency Effects 0.000 claims abstract description 46
- 230000002950 deficient Effects 0.000 claims abstract description 24
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 120
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 104
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 60
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 60
- 239000001569 carbon dioxide Substances 0.000 claims description 60
- 229910052757 nitrogen Inorganic materials 0.000 claims description 46
- 229910001873 dinitrogen Inorganic materials 0.000 claims description 14
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 8
- 229910001882 dioxygen Inorganic materials 0.000 claims description 8
- 238000004364 calculation method Methods 0.000 claims description 6
- 230000009471 action Effects 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 239000013641 positive control Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 abstract description 7
- 230000008569 process Effects 0.000 description 7
- 230000006870 function Effects 0.000 description 5
- 206010021143 Hypoxia Diseases 0.000 description 3
- 230000007954 hypoxia Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000003672 processing method Methods 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000020169 heat generation Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 206010017472 Fumbling Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000590 oncogenic Toxicity 0.000 description 1
- 230000002246 oncogenic effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000759 toxicological effect Toxicity 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Landscapes
- Filling Or Discharging Of Gas Storage Vessels (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a method for detecting gas quantity of a gas cylinder of a three-gas incubator and automatically switching the gas cylinder, which comprises the following steps: step S1: judging the type of gas in the gas cylinder; step S2: according to the type of gas in the gas cylinder, controlling the target concentration by filling corresponding gas into the tank; step S3: judging whether the single gas cylinder is deficient; step S4: switching a working gas cylinder; step S5: and judging whether the gas-lack gas cylinder is replaced by a full gas cylinder manually after all the gas cylinders lack gas. The invention can automatically control and detect the deficiency of the gas in the gas cylinder and timely switch the working gas cylinder, solves the problem of inconvenient concentration control after the replacement of the gas-filled gas cylinder of the three-gas incubator, ensures that the experiment is carried out in a safe state, improves the safety coefficient, saves the cost, and can realize the automatic switching of the high-oxygen and low-oxygen concentration control, thereby saving time and labor.
Description
Technical Field
The invention relates to the field of microorganism experimental equipment, in particular to a method for detecting gas quantity of a gas cylinder of a three-gas incubator and automatically switching the gas cylinder.
Background
A three-gas incubator is a device used in a microbiological laboratory. The temperature, the humidity, the nitrogen concentration, the oxygen concentration and the carbon dioxide concentration in the box are controlled to provide a proper growth environment, so that the method is widely applied to the cultivation and the propagation of microorganisms; study of cell dynamics; collecting secretion of mammalian cells; the oncogenic or toxicological effects of various physical and chemical factors; research and production of antigens; culturing the hybridoma cells to produce antibodies; in Vitro Fertilization (IVF), stem cells, tissue engineering, drug screening, and the like. The biological relevance can be improved by increasing the change of the oxygen concentration environment compared with the simple change of the carbon dioxide concentration environment; increasing cell number and life span; reducing differentiation and stress response; better simulate the tumor microenvironment and provide a more ideal growth environment. The gas normally used in the three-gas incubator is three gases of nitrogen, oxygen and carbon dioxide, so that the gas filling cylinder is a nitrogen cylinder or an oxygen cylinder or a carbon dioxide cylinder. When the incubator needs a hypoxia experiment (0-21% oxygen concentration), a nitrogen bottle and a carbon dioxide bottle are needed to be filled with the gas; when the incubator needs high oxygen experiments (21% -100% oxygen concentration), the oxygen bottle and the carbon dioxide bottle are needed to be filled with the air bottle.
At present, most three-gas incubators do not have gas bottle gas shortage judgment and automatic switching of gas bottle functions, and gas shortage judgment is needed by operators observing pressure gauges on gas bottles; some of the gas cylinders are provided with pressure sensors for detecting the gas shortage, each gas cylinder is required to be provided with one pressure sensor for detecting the pressure, when the pressure is small, the gas shortage is judged, if the pressure is 4 gas cylinders, four pressure sensors are required for detecting the pressure in four ways, and the cost is relatively high and the circuit is troublesome. Some gas cylinder gas deficiency software judging functions are provided, but the gas cylinder gas deficiency software judging functions are not perfect, for example, gas deficiency judgment is only carried out when concentration control is started, and the gas deficiency judgment is not carried out in the concentration control process, so that misjudgment is easily caused if a method is not perfect in the concentration control process. Still other laboratories place three-gas incubators in the laboratory and gas cylinders outside the laboratory, and the replacement of the cylinders is not allowed by the personnel who must then detect that the cylinders have been replaced and automatically resume concentration control after the cylinders have been replaced.
Therefore, there is a strong need for a method that can detect the deficiency of gas in a gas cylinder by a software program and switch the working gas cylinders in time without increasing or reducing the cost, and automatically judge that the gas cylinder that is deficient has been replaced to a full gas cylinder and return to concentration control in time if the gas cylinders are replaced within a prescribed time after all the working gas cylinders are deficient, so as to ensure the experimental safety.
Disclosure of Invention
In view of the above, the present invention aims to provide a method for detecting the gas quantity of a gas cylinder of a three-gas incubator and automatically switching the gas cylinder. The automatic concentration control device can automatically and accurately judge whether the gas in the single gas cylinder is used up, realize automatic switching of the working gas cylinder on the basis of judging that the gas in the single gas cylinder is used up, and can automatically recover concentration control after timely replacing the gas cylinder and carry out alarm prompt when not timely replacing the gas cylinder after judging that all the gas in the gas cylinder is used up.
The invention aims at realizing the following technical scheme:
the method for detecting the gas quantity of the gas cylinder of the three-gas incubator and automatically switching the gas cylinder comprises the following steps:
step S1: judging the type of gas in the gas cylinder; the gas types include carbon dioxide gas, oxygen gas, and nitrogen gas;
step S2: according to the type of the gas in the gas cylinder, PID action control calculation is adopted for the target concentration, and the target concentration is controlled by filling corresponding gas into the tank;
step S3: judging whether the PID control output quantity of the concentration reaches a specified maximum control output quantity, if not, resetting the judgment cycle number of the gas deficiency of the gas cylinder, and returning to the step S2; if yes, adding 1 to the cycle number of the gas cylinder gas deficiency judgment, judging whether the cycle number of the gas cylinder gas deficiency judgment exceeds the set cycle number, if yes, clearing the accumulated cycle number of the gas cylinder gas deficiency judgment, and judging the real-time measurement concentration; judging whether the gas cylinder is in a gas shortage state according to the judgment result of the real-time measured concentration, if the gas cylinder is in a gas shortage state, entering a step S4, and if the gas cylinder is not in a gas shortage state, returning to the step S2; the gas cylinder gas deficiency judging cycle number refers to a PID control cycle requiring several concentrations to judge one gas deficiency;
step S4: resetting an integral term of PID control, switching to the next working gas cylinder, adding 1 to the switching times of the gas cylinders, judging the switching times of the gas cylinders, and returning to the step S2 if the switching times of the gas cylinders are smaller than the set number of the gas cylinders; if the number of the working gas cylinders is equal to the number of the set gas cylinders, the working gas cylinders are required to be switched to the first working gas cylinder, the working gas cylinders are forced to be switched to the first gas cylinder, and then the step S2 is returned;
s5, the working gas cylinder is a first gas cylinder, the switching times of the working gas cylinder are cleared, the specified maximum concentration control output quantity is kept for inflation, whether the working gas cylinder is manually replaced with a full gas cylinder or not is always judged, if the working gas cylinder is not manually replaced with the full gas cylinder within a specified time, concentration control is stopped, and after concentration control is started, gas quantity detection and automatic switching are carried out again; if the full gas cylinder is replaced within the specified time, the concentration integral term is cleared, and the step S2 is returned.
Further: in the step S2, if the gas type determined in the step S1 is carbon dioxide gas or oxygen gas, the target concentration is controlled by charging carbon dioxide gas or oxygen gas into the tank to raise the tank concentration by performing PID reaction control calculation on the target concentration of carbon dioxide or the target concentration of high oxygen (21% -100%).
Further: in the step S3, the value in the minimum concentration unit needs to be updated continuously, and the determination of the deficiency of gas is that the measured real-time concentration value is compared with the value in the stored minimum concentration unit, if the measured real-time concentration value does not rise by 0.5%, the deficiency of gas in the single gas cylinder is indicated, and then the flow of automatically switching the working gas cylinders is entered; if the number of times of switching the working gas cylinders is equal to the set number of gas cylinders, the number of times of switching the working gas cylinders must be cleared, and the judgment is restarted from the first gas cylinder.
Further: in the step S5, the specified maximum concentration control output is maintained for a specified period of time to charge, and the value in the minimum concentration unit is continuously updated, and the method for determining that the gas-deficient gas cylinder is replaced by the gas-filled gas cylinder is that the gas-deficient gas cylinder is replaced by the gas-filled gas cylinder manually by comparing the measured real-time concentration value with the value in the stored minimum concentration unit, if the measured real-time concentration value is not increased by 0.5%, the method indicates that the gas-deficient gas cylinder is not replaced by the gas-filled gas cylinder manually; if the gas rises by at least 0.5%, the gas cylinder which is out of gas is replaced by a gas cylinder which is full of gas by manpower, and the working gas cylinder is not out of gas. The specified time is to ensure that the inflation solenoid valve is not burnt out due to serious heat generation caused by long-term operation.
Alternatively, in the step S2, if the gas type determined in the step S1 is nitrogen, PID positive control calculation is adopted for the target concentration of hypoxia (0% -21%), and the target concentration of hypoxia is controlled by charging nitrogen gas into the tank to dilute the oxygen concentration in the tank.
In this case: in the step S3, the value in the maximum concentration unit needs to be continuously updated, and the determination of the deficiency of gas is that the measured real-time concentration value is compared with the value in the stored maximum concentration value unit, if the measured real-time concentration value is not reduced by 0.5%, the deficiency of gas in the single gas cylinder is indicated, and then the flow of automatically switching the working gas cylinders is entered; if the number of times of switching the working gas cylinders is equal to the set number of gas cylinders, the number of times of switching the working gas cylinders must be cleared, and the judgment is restarted from the first gas cylinder.
Further: in the step S5, the specified maximum concentration control output is maintained for a specified period of time to charge, and the value in the maximum concentration unit is continuously updated, so that the method of judging that the nitrogen cylinder with the gas deficiency is replaced by the nitrogen cylinder with the gas deficiency is that the nitrogen cylinder with the gas deficiency is replaced by the nitrogen cylinder with the gas deficiency manually is judged by comparing the measured real-time concentration value with the value in the stored maximum concentration unit, if the measured real-time concentration value is not reduced by 0.5%, the method indicates that the nitrogen cylinder with the gas deficiency is not replaced by the nitrogen cylinder with the gas deficiency manually; if the pressure drop is at least 0.5%, the condition that the gas-deficient nitrogen cylinder is replaced by a full nitrogen cylinder by manpower is indicated, and the working nitrogen cylinder is not deficient. The specified time is to ensure that the inflation solenoid valve is not burnt out due to serious heat generation caused by long-term operation.
The beneficial effects of the invention are as follows:
(1) The invention can automatically detect the deficiency of the gas in the gas cylinder and timely switch the working gas cylinder, is safe and reliable, does not need manual participation, and reduces the labor input for frequently replacing the gas cylinder;
(2) The number of the gas cylinders can be set according to actual conditions, for example, the designed maximum number of the gas cylinders is 4, and the actual number of the gas cylinders is only 2, so that the number of the gas cylinders can be set to be 2 to meet the actual number.
(3) The problem of inconvenient concentration control after the gas filled cylinders of the three-gas incubator are replaced is solved, because the three-gas incubator is placed in a laboratory, the gas filled cylinders are placed outside the laboratory, and gas cylinder replacement staff are not allowed to enter the laboratory, so that the operation is inconvenient, and the concentration control can be automatically recovered after the gas cylinders are replaced by the method;
(4) The consistency of the concentration control of the three-gas incubator is ensured, the experiment is ensured to be carried out in a safe state, and the safety coefficient is improved;
(5) Compared with the prior art of detecting the deficiency of the gas in the gas cylinder through pressure, the circuit design is simplified, and the cost is saved;
(6) The invention can realize the automatic switching of the high-oxygen concentration control and the low-oxygen concentration control, and the three-gas incubator is switched from the high-oxygen concentration control to the low-oxygen concentration control, and only the oxygen cylinder is replaced by the nitrogen cylinder, thereby saving time and labor.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof.
Drawings
For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings, in which:
FIG. 1 is a diagram of a gas cylinder arrangement of a three-gas incubator;
FIG. 2 is a flow chart for determining the absence of a single carbon dioxide cylinder;
FIG. 3 is a flow chart for determining the absence of a single oxygen cylinder;
FIG. 4 is a flow chart for automatically switching carbon dioxide gas cylinders;
FIG. 5 is a flow chart for automatically switching oxygen cylinders;
FIG. 6 is a flow chart of a post-process procedure after all carbon dioxide cylinders are empty;
FIG. 7 is a flow chart of a post-process procedure after all oxygen cylinders are out of charge;
FIG. 8 is a flow chart for determining the absence of a single nitrogen cylinder;
FIG. 9 is a flow chart for automatically switching nitrogen cylinders;
FIG. 10 is a flow chart showing the process of the post-gas-deficiency procedure for all nitrogen cylinders.
Detailed Description
Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. It should be understood that the preferred embodiments are presented by way of illustration only and not by way of limitation.
Example 1
The gas control of the three-gas incubator mainly comprises carbon dioxide concentration control and oxygen concentration control, wherein the oxygen concentration control is divided into high oxygen concentration (21% -100%) and low oxygen concentration (0% -21%), and the carbon dioxide concentration control and the high oxygen concentration control are carried out by charging gas into the incubator, so that the two methods are the same; the low oxygen concentration control is to dilute the oxygen concentration by charging nitrogen into the tank.
The embodiment of the invention uses a three-gas incubator, and has the functions of controlling the concentration of carbon dioxide and controlling the concentration of high oxygen (21% -100%). As shown in fig. 1, the three-gas incubator is provided with two carbon dioxide gas cylinders and four oxygen gas cylinders, and two solenoid valves of the two carbon dioxide gas cylinders A and B and four solenoid valves of the four oxygen gas cylinders A, B, C, D are respectively controlled by a controller; after the three-gas incubator is electrified for the first time and starts to operate, the gas A cylinder is selected for filling carbon dioxide gas and oxygen gas into the incubator. If the selected gas charging cylinder is the gas cylinder when the operation is stopped last time after the first power-on and the starting operation starts to work, for example, the carbon dioxide is used as the B gas cylinder when the operation is stopped, the B gas cylinder is still used when the operation is restarted, the oxygen cylinder is used as the C oxygen cylinder when the operation is stopped, and the C oxygen cylinder is still used when the operation is restarted.
As shown in fig. 2, the time required for detecting the absence of gas in a single carbon dioxide cylinder is 5 carbon dioxide concentration control cycles, i.e., the parameter C1 is set to 5; in this embodiment, the flow and method for judging the lack of gas in a single carbon dioxide cylinder are as follows: after the carbon dioxide concentration control is started, carbon dioxide gas is filled into the tank to control the carbon dioxide concentration, and a PID reaction control mode is adopted as a control mode. The gas in the carbon dioxide gas cylinder is sufficient, the concentration is continuously increased through PID control output along with the increase of the inflation output, and the concentration control output is turned off after the target concentration is reached; because hundred percent sealing cannot be achieved in the tank, the concentration of the carbon dioxide can be slowly leaked out of the tank, so that the concentration is reduced, after the concentration is reduced, the PID algorithm controls output to automatically supplement air, and the circulation is repeated, so that the carbon dioxide in the gas cylinder is continuously consumed until the gas in the gas cylinder is consumed; after the gas cylinder is out of gas, the concentration is reduced, and the PID control output automatically supplements the gas, but the concentration is continuously reduced due to the gas cylinder out of gas, and the output of a proportional term is continuously increased along with the reduction of the concentration, the output of an integral term is continuously accumulated along with the time and is also continuously increased, and when the sum of the two outputs reaches the maximum output of the specified carbon dioxide concentration, the carbon dioxide measured concentration value at the moment is stored in a minimum concentration unit.
After the maximum output of the carbon dioxide concentration is maintained for 5 concentration control periods, the cycle number of concentration accumulation is cleared, and whether the actual measured concentration value and the value stored in the minimum concentration unit are raised by 0.5% is judged. When the maximum carbon dioxide concentration output is maintained for 5 concentration control periods, if the gas is deficient in the gas cylinder, the concentration will not rise but fall, and at this time, it is certainly judged that the gas is deficient. In order to prevent erroneous determination when concentration control is started, a condition is set such that the maximum output of carbon dioxide concentration is maintained for 5 concentration control periods without an increase of 0.5%, and gas shortage is determined. If the maximum output is inflated just when the concentration control is started because the three-gas incubator is large in volume, the maximum output is still not increased by 0.5% in 5 concentration control periods, and misjudgment occurs, and the solution is to increase the C1 parameter, for example, from 5 to 8.
According to the illustration in fig. 3, the time required for detecting the lack of a single oxygen cylinder is 10 oxygen concentration control cycles, i.e., the parameter C2 is set to 10; the method and the flow for judging the lack of the oxygen in the single oxygen cylinder through the program are consistent with the carbon dioxide, and the repeated description is omitted here.
According to the method shown in FIG. 4, the number of the carbon dioxide cylinders of the three-gas incubator is set to 2, namely, the parameter n1 is set to 2, and the two cylinders are respectively a carbon dioxide cylinder A and a carbon dioxide cylinder B, and when the three-gas incubator detects that a single carbon dioxide cylinder is out of gas through software, the working cylinder is automatically switched to the next cylinder through the software, and the method and the flow are as follows: after the working gas cylinder lacks gas, the integral term output of the carbon dioxide concentration must be cleared, and then the working gas cylinder is automatically switched to the next gas cylinder, and the number of times of switching gas cylinders is increased by 1. For example, the gas cylinder A is in short of gas, and then is automatically switched to the gas cylinder B to be used as a working gas cylinder, and the switching times of the gas cylinder are 1; for example, the B gas cylinder is out of gas, and is automatically switched to the A gas cylinder to be used as a working gas cylinder, and the switching times of the gas cylinder are 2. If the gas bottle A is no longer deficient, the number of times of switching the gas bottle must be reset to 0; if the A gas cylinder is deficient again, the switching times of the gas cylinders are 3. The number of times of switching the gas cylinders is equal to the set number of gas cylinders, and then the working gas cylinder is required to be switched to the A gas cylinder; the number of times of switching the gas cylinders is greater than or equal to the set number of the gas cylinders n1+1=3, which indicates that the gas cylinder A is out of gas twice, namely, after the gas cylinder A is out of gas for the first time, the gas cylinder A which is out of gas is not replaced by the gas cylinder A which is full of gas through manual work, at the moment, all the gas cylinders are prompted to be out of gas, the gas cylinders which are full of gas need to be replaced manually, and then the program processing after all the carbon dioxide gas cylinders are out of gas is carried out. This step is very critical, because according to actual conditions, after the gas of the general A gas cylinder lacks, the working gas cylinder is switched to the B gas cylinder, and the worker sees that the gas of the A gas cylinder is used up, and the A gas cylinder can be replaced, and if the judgment of the A gas cylinder is not carried out for the second time, the misjudgment that the A gas cylinder is full but the gas deficiency of all the gas cylinders is prompted can occur. When the working gas cylinder is switched, the accumulated integral output item is cleared, which is equivalent to that after the integral item is reset, the integral item is accumulated again from 0, and the proportional item output is taken as the initial inflation output quantity. In this way, the switching initial charge output is small, and the gas in the tank does not overshoot or overshoot is not excessively large.
According to the method shown in fig. 5, the number of oxygen cylinders in the three-gas incubator is set to 4, i.e. the parameter n2 is set to 4, and the four cylinders are A, B, C, D oxygen cylinders respectively, and the method and the process for automatically switching the oxygen working cylinder to the next oxygen cylinder through software are also consistent with those of the automatic switching working cylinder of carbon dioxide, and the repeated description is omitted here.
According to the method for processing the program after the carbon dioxide A, B gas cylinders of the three-gas incubator are all used up, as shown in fig. 6, the method and the flow for automatically recovering concentration control and alarming prompt after not replacing the gas cylinders in time if a worker replaces the gas cylinders in time within a specified time are as follows: the prescribed time for replacing the carbon dioxide gas cylinder is set to be 4 hours, after the AB gas cylinder is out of gas, the A gas cylinder is used as a working gas cylinder, the maximum output of the inflating electromagnetic valve of the A gas cylinder is kept to be opened, then the concentration value of the carbon dioxide is always detected, the value is smaller than the value in the minimum concentration value unit, and the value in the minimum concentration value unit is updated. After the working personnel replace the gas cylinder within four hours, the gas in the working gas cylinder is full and always acts with the maximum gas filling output, so the gas cylinder can be quickly raised by 0.5% or directly exceeds the target concentration on the basis of the minimum concentration value, the gas cylinder is judged to have been replaced, the integral output item of the concentration is cleared, the integral output item is equivalent to the integral output item is reset and then is accumulated from 0, the output of the proportional item is used as the initial gas filling output quantity, and the gas shortage judgment of the single gas cylinder of the next round is carried out. After long-time experiments and fumbling by the inventors, the prescribed time is preferably set to 4 hours, and the decrease in the carbon dioxide concentration value within 4 hours does not have a great influence on the experiments. If the gas cylinder is not replaced for more than 4 hours, stopping concentration control, closing an inflation electromagnetic valve of the A gas cylinder, and adopting a buzzer or other audible and visual equipment to alarm to prompt that all the gas cylinders are out of gas.
As shown in fig. 7, the procedure processing method after the four oxygen cylinders of the three-gas incubator A, B, C, D are all used up includes that if the worker replaces the oxygen cylinders in time within a specified period of time, the method and the procedure for automatically recovering the concentration control and alarming prompt after not replacing the oxygen cylinders in time are also consistent with the procedure processing method after the carbon dioxide AB gas cylinders are all used up, and the detailed description is not repeated here.
Example two
In this example, a three-gas incubator is used, which has carbon dioxide concentration control and low oxygen concentration (0-21%) control functions. As shown in fig. 1, the three-gas incubator is provided with two carbon dioxide gas cylinders and four nitrogen gas cylinders, and two solenoid valves of the two carbon dioxide gas cylinders A and B and four solenoid valves of the four nitrogen gas cylinders A, B, C, D are respectively controlled by a controller; after the three-gas incubator is electrified for the first time and starts to operate, the gas cylinder A is selected firstly by filling carbon dioxide gas and nitrogen gas into the incubator. If the selected gas charging cylinder is the gas cylinder when the operation is stopped last time after the first power-on and the starting operation starts to work, for example, the carbon dioxide is used as the B gas cylinder when the operation is stopped, the B gas cylinder is still used when the operation is restarted, the nitrogen gas cylinder is used as the C nitrogen gas cylinder when the operation is stopped, and the C nitrogen gas cylinder is still used when the operation is restarted.
According to the method and the flow for judging the deficiency of the single carbon dioxide gas cylinder by the software shown in fig. 2, the flow and the method are consistent with the embodiment, and the repeated description is omitted;
according to the method and the process for automatically switching the carbon dioxide working gas cylinder to the next gas cylinder in the three-gas incubator through software shown in fig. 4, the method and the process remain consistent with the embodiment, and the repeated description is omitted;
according to the method shown in fig. 6, the procedure processing method after the carbon dioxide A, B cylinders of the three-gas incubator are all used up includes that if the worker replaces the cylinders in time within a specified time, the concentration control is automatically restored, and the alarm prompt method and the alarm prompt process after the replacement are not timely consistent with the embodiment, and the detailed description is not repeated;
as shown in fig. 8, in the present embodiment, the time required to detect the lack of gas in a single nitrogen cylinder is 5 nitrogen concentration control cycles, i.e., the parameter C2 is set to 5; the flow and method for judging the gas shortage of the single nitrogen cylinder through the software are as follows: after the nitrogen concentration control is started, nitrogen gas is filled into the box to control the oxygen concentration, and the oxygen concentration in the box is diluted by filling nitrogen gas, so that a PID positive control mode is adopted as the control mode. The gas in the nitrogen cylinder is sufficient, the oxygen concentration is continuously reduced through PID control output along with the increase of the inflation output, and the oxygen concentration control output is closed after the target oxygen concentration is reached; because hundred percent sealing cannot be achieved in the tank, the oxygen concentration can slowly leak out of the tank, so that the oxygen concentration rises, after the oxygen concentration rises, the PID algorithm controls output to automatically charge nitrogen and the circulation is repeated, so that the nitrogen gas in the gas cylinder is continuously consumed until the gas in the gas cylinder is depleted; after the gas cylinder is out of gas, the oxygen concentration rises, and the PID control outputs an automatic charging action, but the concentration continues to rise due to the gas cylinder out of gas, and the output of a proportional term is continuously increased along with the rising of the concentration, the output of an integral term is continuously accumulated along with the time and is also continuously increased, and when the sum of the two outputs reaches the maximum output of the specified oxygen concentration, the oxygen measurement concentration value at the moment is stored in a maximum concentration unit. After the maximum output of the oxygen concentration is maintained for 5 concentration control periods, the cycle number of concentration accumulation is cleared, and whether the actual measured concentration value and the value stored in the maximum concentration unit are reduced by 0.5% or not is judged. At the maximum output of the oxygen concentration and with 5 concentration control periods maintained, if the gas is deficient in the gas cylinder, the concentration can not only be reduced, but also be increased, and at the moment, the gas deficiency of the nitrogen gas cylinder can be judged certainly. In order to prevent erroneous determination immediately after the start of the concentration control, the condition was set so that the oxygen concentration maximum output was maintained for 5 concentration control periods without decreasing by 0.5%, and the determination was made as the lack of gas. If the three-gas incubator is large in volume, the maximum output is inflated immediately after the concentration control is started, but the three-gas incubator still does not drop by 0.5% in 5 concentration control periods, and erroneous judgment occurs. The solution is to increase the C2 parameter, for example from 5 to 8.
As shown in FIG. 9, the number of nitrogen cylinders of the three-gas incubator is set to be 4, namely, the parameter n2 is set to be 4, two cylinders are respectively A, B, C, D four nitrogen cylinders, and when the three-gas incubator detects that a single nitrogen cylinder is out of gas through software, the method and the flow path for automatically switching the working cylinder to the next cylinder through the software are as follows: after the working gas cylinder lacks gas, the integral term output of the oxygen concentration must be cleared, and then the working gas cylinder is automatically switched to the next gas cylinder, and the number of times of switching gas cylinders is increased by 1. For example, the gas cylinder A is in short of gas, and then is automatically switched to the gas cylinder B to be used as a working gas cylinder, and the switching times of the gas cylinder are 1; for example, the B gas cylinder is in short of gas, the C gas cylinder is automatically switched to be used as a working gas cylinder, the switching times of the gas cylinder are 2, for example, the D gas cylinder is in short of gas, the A gas cylinder is automatically switched to be used as the working gas cylinder, and the switching times of the gas cylinder are 4. If the gas bottle A is no longer deficient, the number of times of switching the gas bottle must be reset to 0; if the A gas cylinder is deficient again, the switching times of the gas cylinders are 5. The number of times of switching the working gas cylinders is equal to the set number of gas cylinders, and the working gas cylinders must be switched to the A gas cylinders; the number of times of switching the gas cylinders is greater than or equal to the set number of the gas cylinders n2+1=5, which indicates that the gas cylinder A is out of gas twice, namely, after the gas cylinder A is out of gas for the first time, the gas cylinder A which is out of gas is not replaced by the gas cylinder A which is full of gas through manual work, at the moment, all the gas cylinders are prompted to be out of gas, the gas cylinders which are full of gas need to be replaced manually, and then the program processing after all the nitrogen cylinders are out of gas is carried out. This step is very critical because according to the actual situation, after the gas of the A gas cylinder is generally out of supply, the working gas cylinder is switched to the B gas cylinder, and the worker sees that the gas of the A gas cylinder is used up and can replace the A gas cylinder. If the judgment of the A gas cylinder is not carried out for the second time, the misjudgment that the A gas cylinder is full but the gas cylinders are lack of gas is prompted. When the working gas cylinder is switched, the accumulated integral output item is cleared, which is equivalent to that after the integral item is reset, the integral item is accumulated again from 0, and the proportional item output is taken as the initial inflation output quantity. In this way, the switching initial charge output is small, and the gas in the tank does not overshoot or overshoot is not excessively large.
As shown in fig. 10, the method for processing the program after the four nitrogen cylinders of the three-gas incubator A, B, C, D are all used up comprises the following steps of automatically recovering concentration control if the cylinders are replaced in time within a specified time and alarming prompt after the replacement is not completed in time: the prescribed time for replacing the nitrogen cylinder is set to be 4 hours, after all the four cylinders A, B, C, D are out of gas, the A cylinder is used as a working cylinder, the maximum output of the inflation electromagnetic valve of the A cylinder is kept open, then the oxygen concentration value is always detected and is larger than the value in the maximum concentration value unit, and the value in the maximum concentration value unit is updated. After the working personnel replace the gas cylinder within four hours, the gas in the working gas cylinder is full and always acts with the maximum gas filling output, so the gas cylinder can be quickly lowered by 0.5 percent or reach or lower than the target concentration on the basis of the maximum concentration value, the gas cylinder is judged to have been replaced, the integral output item of the concentration is cleared, the integral output item is equivalent to the integral output item which is reset and then is accumulated from 0, the output of the proportional item is taken as the initial gas filling output quantity, and the gas shortage judgment of the single gas cylinder of the next round is carried out. As described above, the predetermined time is preferably set to 4 hours, and the decrease in the oxygen concentration value within 4 hours does not have a large influence on the experiment. If the gas cylinders are not replaced for more than 4 hours, stopping concentration control, closing the inflation electromagnetic valve of the A gas cylinder, and alarming to prompt that all the gas cylinders are out of gas.
It should be emphasized that the various embodiments described herein are merely exemplary in a further processing manner, and that each embodiment is merely set forth for a specific purpose of describing a different embodiment from the other embodiments. For the device disclosed in the embodiments, since it corresponds to the method disclosed in the embodiments, the description is not limited to the description of the method section.
Those of skill would further appreciate that the various illustrative elements and algorithm steps described in connection with the embodiments disclosed herein may be implemented as electronic hardware, computer software, or combinations of both, and that the various illustrative elements and steps are described above generally in terms of functionality in order to clearly illustrate the interchangeability of hardware and software. Whether such functionality is implemented as hardware or software depends upon the particular application and design constraints imposed on the solution. Skilled artisans may implement the described functionality in varying ways for each particular application, but such implementation decisions should not be interpreted as causing a departure from the scope of the present invention.
The steps of a method or algorithm described in connection with the embodiments disclosed herein may be embodied directly in hardware, in a software module executed by a processor, or in a combination of the two. The software modules may be disposed in Random Access Memory (RAM), memory, read Only Memory (ROM), electrically programmable ROM, electrically erasable programmable ROM, registers, hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art.
Finally, it is noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the present invention, which is intended to be covered by the claims of the present invention.
Claims (7)
1. The method for detecting the gas quantity of the gas cylinder of the three-gas incubator and automatically switching the gas cylinder is characterized by comprising the following steps of: the method comprises the following steps:
step S1: judging the type of gas in the gas cylinder; the gas types include carbon dioxide gas, oxygen gas, and nitrogen gas;
step S2: according to the type of the gas in the gas cylinder, PID action control calculation is adopted for the target concentration, and the target concentration is controlled by filling corresponding gas into the tank;
step S3: judging whether the PID control output quantity of the concentration reaches a specified maximum control output quantity, if not, resetting the judgment cycle number of the gas deficiency of the gas cylinder, and returning to the step S2; if yes, adding 1 to the cycle number of the gas cylinder gas deficiency judgment, judging whether the cycle number of the gas cylinder gas deficiency judgment exceeds the set cycle number, if yes, clearing the accumulated cycle number of the gas cylinder gas deficiency judgment, and judging the real-time measurement concentration; judging whether the gas cylinder is in a gas shortage state according to the judgment result of the real-time measured concentration, if the gas cylinder is in a gas shortage state, entering a step S4, and if the gas cylinder is not in a gas shortage state, returning to the step S2; the gas cylinder gas deficiency judging cycle number refers to a PID control cycle requiring several concentrations to judge one gas deficiency;
step S4: resetting an integral term of PID control, switching to the next working gas cylinder, adding 1 to the switching times of the gas cylinders, judging the switching times of the gas cylinders, and returning to the step S2 if the switching times of the gas cylinders are smaller than the set number of the gas cylinders; if the number of the working gas cylinders is equal to the number of the set gas cylinders, the working gas cylinders are forcedly switched to the first gas cylinder, and then the step S2 is returned; if the number of the gas cylinders is larger than the set number, continuing to lack gas after switching to the first working gas cylinder again, wherein the gas deficiency represents that all the gas cylinders lack gas, and then entering step S5;
s5, the working gas cylinder is a first gas cylinder, the switching times of the working gas cylinder are cleared, the specified maximum concentration control output quantity is kept for inflation, whether the working gas cylinder is replaced with a full gas cylinder is always judged, if the working gas cylinder is not replaced with the full gas cylinder within a specified time, concentration control is stopped, gas quantity detection is carried out again after concentration control is started, and the gas cylinder is automatically switched; if the full gas cylinder is replaced within the specified time, resetting the concentration integral term, and returning to the step S2;
if the judgment in the step S1 is that the carbon dioxide gas or the oxygen gas is detected, the gas deficiency judgment is that the measured real-time concentration value is compared with the value in the stored minimum concentration unit, and if the value does not rise by 0.5%, the gas deficiency of the single gas cylinder is indicated; if the working gas cylinder rises by at least 0.5%, the working gas cylinder is not deficient; the method for judging whether the gas-deficient gas cylinder is replaced by the gas-full gas cylinder is that the measured real-time concentration value is compared with the value in the stored minimum concentration unit, if the value is not increased by 0.5%, the gas-deficient gas cylinder is not replaced by the gas-full gas cylinder; if at least 0.5% rise, it is indicated that the gas-deficient cylinder has been replaced with a full cylinder;
if the gas type in the gas cylinder judged in the step S1 is nitrogen, judging that the gas is deficient by comparing the measured real-time concentration value with the value in the stored maximum concentration value unit, and if the gas is not reduced by 0.5%, indicating that the gas in the single gas cylinder is deficient; if the pressure drop is at least 0.5%, the working gas cylinder is not deficient; the method for judging whether the nitrogen cylinder with the gas deficiency is replaced by the nitrogen cylinder with the gas deficiency is that the measured real-time concentration value is compared with the value in the stored maximum concentration unit, if the value is not reduced by 0.5%, the judgment that the nitrogen cylinder with the gas deficiency is not replaced by the nitrogen cylinder with the gas deficiency is made; if the drop is at least 0.5%, this indicates that the nitrogen cylinder that is out of gas has been replaced with a nitrogen cylinder that is full of gas.
2. The method for detecting the gas quantity of the gas cylinder of the three-gas incubator and automatically switching the gas cylinder according to claim 1, wherein the method comprises the following steps: in the step S2, if the gas type determined in the step S1 is carbon dioxide gas or oxygen, the target concentration is controlled by charging carbon dioxide gas or oxygen into the tank to raise the tank concentration by PID reaction control calculation to be performed on the target concentration of carbon dioxide or the target concentration of high oxygen.
3. The method for detecting the gas quantity of the gas cylinder of the three-gas incubator and automatically switching the gas cylinder according to claim 2, wherein the method comprises the following steps of: in the step S3, the value in the minimum concentration unit needs to be updated continuously, and the determination of the deficiency of gas is that the measured real-time concentration value is compared with the value in the stored minimum concentration unit, if the measured real-time concentration value does not rise by 0.5%, the deficiency of gas in the single gas cylinder is indicated, and then the flow of automatically switching the working gas cylinders is entered; if the number of times of switching the working gas cylinders is equal to the set number of gas cylinders, the number of times of switching the working gas cylinders must be cleared, and the judgment is restarted from the first gas cylinder.
4. A method for detecting gas quantity and automatically switching gas cylinders of a three-gas incubator according to claim 2 or 3, characterized in that: in the step S5, the predetermined maximum concentration control output is maintained for a predetermined period of time, and the value in the minimum concentration unit is continuously updated, and the method for judging whether the gas-deficient gas cylinder is replaced by the gas-filled gas cylinder is to compare the measured real-time concentration value with the value in the stored minimum concentration unit, and if the measured real-time concentration value is not increased by 0.5%, the method indicates that the gas-deficient gas cylinder is not replaced by the gas-filled gas cylinder; if at least 0.5% rise, this indicates that the gas cylinder that is out of gas has been replaced with a gas cylinder that is full of gas, and that the working cylinder is not out of gas.
5. The method for detecting the gas quantity of the gas cylinder of the three-gas incubator and automatically switching the gas cylinder according to claim 1, wherein the method comprises the following steps: in the step S2, if the gas type in the gas cylinder determined in the step S1 is nitrogen, PID positive control calculation is adopted for the low oxygen target concentration, and the low oxygen target concentration is controlled by charging nitrogen gas into the tank to dilute the oxygen concentration in the tank.
6. The method for detecting the gas quantity of the gas cylinder of the three-gas incubator and automatically switching the gas cylinder according to claim 5, wherein the method comprises the following steps: in the step S3, the value in the maximum concentration unit needs to be continuously updated, and the determination of the deficiency of gas is that the measured real-time concentration value is compared with the value in the stored maximum concentration value unit, if the measured real-time concentration value is not reduced by 0.5%, the deficiency of gas in the single gas cylinder is indicated, and then the flow of automatically switching the working gas cylinders is entered; if the number of times of switching the working gas cylinders is equal to the set number of gas cylinders, the number of times of switching the working gas cylinders must be cleared, and the judgment is restarted from the first gas cylinder.
7. The method for detecting the gas quantity of the three-gas incubator gas cylinder and automatically switching the gas cylinder according to claim 5 or 6, wherein: in the step S5, the specified maximum concentration control output is maintained for a specified period of time, and the value in the maximum concentration unit is continuously updated, so that the method of judging whether the nitrogen cylinder with the deficiency is replaced by the nitrogen cylinder with the full charge is that the measured real-time concentration value is compared with the value in the stored maximum concentration unit, and if the measured real-time concentration value is not reduced by 0.5%, the method indicates that the nitrogen cylinder with the deficiency is not replaced by the nitrogen cylinder with the full charge; if the pressure drops by at least 0.5%, the nitrogen cylinder which is out of gas is replaced by the nitrogen cylinder which is full of gas, and the working nitrogen cylinder is not out of gas.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311520273.4A CN117230259B (en) | 2023-11-15 | 2023-11-15 | Method for detecting gas quantity of gas cylinder of three-gas incubator and automatically switching gas cylinders |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311520273.4A CN117230259B (en) | 2023-11-15 | 2023-11-15 | Method for detecting gas quantity of gas cylinder of three-gas incubator and automatically switching gas cylinders |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117230259A CN117230259A (en) | 2023-12-15 |
| CN117230259B true CN117230259B (en) | 2024-01-23 |
Family
ID=89086559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311520273.4A Active CN117230259B (en) | 2023-11-15 | 2023-11-15 | Method for detecting gas quantity of gas cylinder of three-gas incubator and automatically switching gas cylinders |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117230259B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118502491A (en) * | 2024-04-28 | 2024-08-16 | 青岛威巴克生物技术有限公司 | Air supply control method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02120019U (en) * | 1989-03-15 | 1990-09-27 | ||
| CN201420082Y (en) * | 2009-05-08 | 2010-03-10 | 上海力申科学仪器有限公司 | Automatic gas storage bottle switching device for incubator |
| JP2015098896A (en) * | 2013-11-19 | 2015-05-28 | ヤマト産業株式会社 | Automatic switching device for gas supply |
| CN211367567U (en) * | 2019-10-18 | 2020-08-28 | 浙江泰林医学工程有限公司 | Automatic switching device for air source of carbon dioxide incubator |
| CN115248606A (en) * | 2022-06-28 | 2022-10-28 | 青岛海尔生物医疗科技有限公司 | Control method of gas supply system and gas supply system |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7019656B2 (en) * | 2002-08-06 | 2006-03-28 | Kendro Laboratory Products, Inc. | Empty gas supply tank pending warning |
-
2023
- 2023-11-15 CN CN202311520273.4A patent/CN117230259B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02120019U (en) * | 1989-03-15 | 1990-09-27 | ||
| CN201420082Y (en) * | 2009-05-08 | 2010-03-10 | 上海力申科学仪器有限公司 | Automatic gas storage bottle switching device for incubator |
| JP2015098896A (en) * | 2013-11-19 | 2015-05-28 | ヤマト産業株式会社 | Automatic switching device for gas supply |
| CN211367567U (en) * | 2019-10-18 | 2020-08-28 | 浙江泰林医学工程有限公司 | Automatic switching device for air source of carbon dioxide incubator |
| CN115248606A (en) * | 2022-06-28 | 2022-10-28 | 青岛海尔生物医疗科技有限公司 | Control method of gas supply system and gas supply system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117230259A (en) | 2023-12-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN117230259B (en) | Method for detecting gas quantity of gas cylinder of three-gas incubator and automatically switching gas cylinders | |
| EP2816890B1 (en) | Method and apparatus for controlling the atmosphere in a space filled with agricultural or horticultural produce | |
| CN103080862B (en) | Utilize the forbay method for controlling water level of the piping system of level sensor and surface cock | |
| EP2321403B1 (en) | A predictor of when to harvest cells grown in a bioreactor | |
| CN108368471B (en) | Identification of calibration offset for PH measuring devices | |
| CN102229888A (en) | Feedback-type pneumatic-control pressure stress cell culture device | |
| CN115248606A (en) | Control method of gas supply system and gas supply system | |
| CN211367567U (en) | Automatic switching device for air source of carbon dioxide incubator | |
| CN206345845U (en) | A kind of modified Pit sealing sealing device | |
| CN217366601U (en) | Steam sterilizer for detecting and sterilizing new crown nucleic acid | |
| US6831564B2 (en) | Low supply tank pressure warning | |
| CN215414407U (en) | Sterilization effect inspection auxiliary device of continuous type active toxicity effluent disposal system | |
| CN116948817A (en) | Disposable biological safety bioreactor and monitoring method | |
| CN208933383U (en) | A kind of intelligent automation cell colony experimental facilities | |
| CN113607454A (en) | Sterilization effect inspection auxiliary device and method for continuous live-toxicity wastewater treatment system | |
| CN205300878U (en) | Biological indicator equipment | |
| CN211367585U (en) | Butt-joint carbon dioxide culture system | |
| CN114752646A (en) | Method for detecting sulfate reducing bacteria | |
| CN206219584U (en) | A kind of suspension cell lesion terminal automatic identification and control system | |
| CN221141744U (en) | Fermentation pressure control device and fermentation equipment | |
| CN218916731U (en) | Test system for bottle valve | |
| CN213475935U (en) | Intelligent anaerobic incubator | |
| CN104062985B (en) | Water level control device and method of sampling system | |
| WO2024135332A1 (en) | Chlorine dioxide concentration control system | |
| CN116759117A (en) | Automatic nitrogen supplementing system of heat supply reactor and control method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |