CN117229980B - Weissella sinica fermented feed and application and deodorization effect thereof - Google Patents
Weissella sinica fermented feed and application and deodorization effect thereof Download PDFInfo
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- 241000202221 Weissella Species 0.000 title claims abstract description 46
- 238000004332 deodorization Methods 0.000 title description 5
- 230000000694 effects Effects 0.000 title description 5
- 235000013305 food Nutrition 0.000 claims abstract description 13
- 238000009629 microbiological culture Methods 0.000 claims abstract description 5
- 238000000855 fermentation Methods 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 241001465754 Metazoa Species 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 4
- 238000003975 animal breeding Methods 0.000 claims description 3
- 239000002781 deodorant agent Substances 0.000 claims description 2
- 239000000654 additive Substances 0.000 claims 4
- 230000000996 additive effect Effects 0.000 claims 4
- 230000001877 deodorizing effect Effects 0.000 abstract description 7
- 241000975185 Weissella cibaria Species 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 2
- 239000002912 waste gas Substances 0.000 abstract description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
- 238000012360 testing method Methods 0.000 description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 230000012010 growth Effects 0.000 description 9
- 239000002253 acid Substances 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 8
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- 210000003608 fece Anatomy 0.000 description 7
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- 241000282887 Suidae Species 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 239000003833 bile salt Substances 0.000 description 5
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- IWHLYPDWHHPVAA-UHFFFAOYSA-N 6-hydroxyhexanoic acid Chemical compound OCCCCCC(O)=O IWHLYPDWHHPVAA-UHFFFAOYSA-N 0.000 description 3
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- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
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- 238000000338 in vitro Methods 0.000 description 3
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- JJMDCOVWQOJGCB-UHFFFAOYSA-N 5-aminopentanoic acid Chemical compound [NH3+]CCCCC([O-])=O JJMDCOVWQOJGCB-UHFFFAOYSA-N 0.000 description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
- 229930182821 L-proline Natural products 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 229940024606 amino acid Drugs 0.000 description 2
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- LVRFTAZAXQPQHI-UHFFFAOYSA-N 2-hydroxy-4-methylvaleric acid Chemical compound CC(C)CC(O)C(O)=O LVRFTAZAXQPQHI-UHFFFAOYSA-N 0.000 description 1
- KJHJAABRDASKAF-UHFFFAOYSA-N 3,7-dihydropurine-2,6-dione Chemical compound OC1=NC(O)=C2N=CNC2=N1.O=C1NC(=O)NC2=C1NC=N2 KJHJAABRDASKAF-UHFFFAOYSA-N 0.000 description 1
- JVGVDSSUAVXRDY-UHFFFAOYSA-M 3-(4-hydroxyphenyl)lactate Chemical compound [O-]C(=O)C(O)CC1=CC=C(O)C=C1 JVGVDSSUAVXRDY-UHFFFAOYSA-M 0.000 description 1
- JVGVDSSUAVXRDY-UHFFFAOYSA-N 3-(4-hydroxyphenyl)lactic acid Chemical compound OC(=O)C(O)CC1=CC=C(O)C=C1 JVGVDSSUAVXRDY-UHFFFAOYSA-N 0.000 description 1
- BYUOBSUZYQAFJM-UHFFFAOYSA-N 6,8-Dihydroxypurine Chemical compound N1C=NC(=O)C2=C1NC(=O)N2 BYUOBSUZYQAFJM-UHFFFAOYSA-N 0.000 description 1
- KNVADAPHVNKTEP-CIGXQKLNSA-N 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid Chemical compound C([C@H]1C[C@H]2O)C(=O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)CC1 KNVADAPHVNKTEP-CIGXQKLNSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
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- 229930195714 L-glutamate Natural products 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- PIPJWVLVHFNOQG-HRYDZAOHSA-N Simonin IV Natural products O=C(O[C@@H]1[C@@H](O[C@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](C)O2)[C@H](O[C@H]2[C@@H](O)[C@@H](O)[C@H](OC(=O)CCCCCCCCCCC)[C@@H](C)O2)[C@@H](C)O[C@H]1O[C@H]1[C@H](C)O[C@@H]2O[C@@H]3[C@H](O)[C@@H](O)[C@H](C)O[C@@H]3O[C@@H](CCCCC)CCCCCCCCCC(=O)O[C@H]2[C@@H]1O)CCCCCCCCC PIPJWVLVHFNOQG-HRYDZAOHSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- DLAMVQGYEVKIRE-UHFFFAOYSA-N alpha-(methylamino)isobutyric acid Chemical compound CNC(C)(C)C(O)=O DLAMVQGYEVKIRE-UHFFFAOYSA-N 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 235000019730 animal feed additive Nutrition 0.000 description 1
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- WCZVZNOTHYJIEI-UHFFFAOYSA-N cinnoline Chemical compound N1=NC=CC2=CC=CC=C21 WCZVZNOTHYJIEI-UHFFFAOYSA-N 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
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- 229960000956 coumarin Drugs 0.000 description 1
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- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
- 229960003964 deoxycholic acid Drugs 0.000 description 1
- KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
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- 239000011521 glass Substances 0.000 description 1
- 229960002989 glutamic acid Drugs 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
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- UMRZSTCPUPJPOJ-KNVOCYPGSA-N norbornane Chemical compound C1C[C@H]2CC[C@@H]1C2 UMRZSTCPUPJPOJ-KNVOCYPGSA-N 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
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- 150000007524 organic acids Chemical class 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 125000003386 piperidinyl group Chemical group 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
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- 238000001179 sorption measurement Methods 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides Weissella shikimchi and application and deodorizing effects thereof. Wherein, the strain of the Weissella antrum has been deposited in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, including Weissella antrum Weissella cibaria, with a deposit number of: CGMCC No.26854. The Weissella food provided by the invention has a deodorizing effect on waste gas in a farm, and has a good development and application prospect, and the Weissella food provides a novel reagent or deodorizing way for reducing/removing odor in the farm.
Description
Technical Field
The invention belongs to the technical field of animal feed additives, and particularly relates to Weissella shirtz, and application and deodorizing effects thereof.
Background
At present, the animal husbandry is gradually changed from the increase of the quantity to the high-quality green low-carbon development with serious economic, ecological and social benefits, and the existing live pig production meets the requirements of people on pork products, but a large amount of malodorous gas can be generated in the pig raising process, so that the health of pig groups is influenced, the production performance is reduced, and the health of raising personnel and the surrounding resident environment are also threatened. The research on the mechanism and nutrition regulation measures for generating the pig group odor has important guiding significance and application value for pig raising production in China.
The lactobacillus preparation is used as a novel green feed additive, has the advantages of safety, high quality and no toxic or side effect, is more and more valued by people, and has good application prospect. Lactic acid bacteria can promote pig nutrient absorption by producing organic acid and beneficial metabolites, and reduce fermentation substrates of colon microorganisms, which is a safe and feasible way to reduce malodorous gas generation from the source compared with physical adsorption or chemical agent deodorization and other methods. Weissella (Weissella) belongs to the order of lactic acid bacteria, and fermentation products of Weissella are various and have different functions, can be used in food processing processes, improve product quality and product characteristics, and have wide research significance. Weissella is a heterofermentation type lactic acid strain, and the heterofermentation type strain can produce substances such as acetic acid, mannitol and the like besides lactic acid, so that the diversity of fermentation products is increased (Zhang Nandi and the like, 2018). At present, the Weissella is mainly used for food and soy sauce fermentation systems, has a regulating effect on amino acid utilization, and has no report and research on the aspects of pig raising production and odor reduction.
Disclosure of Invention
In order to solve the technical problems, the invention provides a Weissella sinus edible fermented feed and application thereof in animal cultivation deodorization.
In order to achieve the above purpose, the invention adopts the following technical scheme:
weissella antrum, which has been deposited in China general microbiological culture Collection center under the accession number Weissella antrum Weissella cibaria, at 2023, 03, 22, month: CGMCC No.26854.
The use of antral Weissella as described above in the preparation of an animal farming deodorant.
A fermented feed for reducing animal cultivation odor is obtained by fermenting complete feed with the antrum weissella.
Preferably, the inoculation amount of the antral Weissella in the complete feed is 0.30% -0.50% of the weight of the complete feed.
The fermented feed is preferably added in an amount of 0.8-3% by weight of the feed.
Further, the addition amount of the fermented feed in the feed is 1% of the weight of the feed.
A feed for reducing animal breeding malodor comprising the aforementioned antral weissella.
The invention has the beneficial effects that:
the fermented feed for the antral Weissella (CGMCC No. 26854) has a deodorizing effect on waste gas in a farm, has good development and application prospects, can promote pig group growth, reduce emission of ammonia and hydrogen sulfide odor, reduce degradation of tryptophan to form manure stink, and provides a new reagent or deodorizing way for reducing/removing odor in the farm.
Drawings
FIG. 1 is a photograph showing colony morphology of Weissella food;
FIG. 2 is a gram stain of Weissella food;
FIG. 3 is a graph showing the growth of Weissella food ZWC 030;
FIG. 4 is a graph showing that Weissella food ZWC030 affects fecal metabonomics analysis;
FIG. 5 is CO 2 Plot of yield;
FIG. 6 is H 2 Plot of S yield;
FIG. 7 is NH 3 Plot of yield.
Detailed Description
The following examples serve to further illustrate the invention but are not to be construed as limiting the invention. Modifications and substitutions made to the invention without departing from the spirit and nature of the invention are intended to be within the scope of the invention.
Unless otherwise indicated, all technical means used in the examples are conventional means well known to those skilled in the art, and unless otherwise specified, all reagents used in the methods are of analytical purity or above.
EXAMPLE 1 isolation, screening and identification of Weissella
With NH 4 Cl is taken as a nitrogen source of a culture medium, fresh manure of growing and fattening pigs in a hilly farm is collected, 25g of the manure is weighed and placed in a triangular flask with glass beads and filled with 225ml of sterile physiological saline, and the fungus solution is obtained by full shaking and uniform shaking. Sucking 1.0ml of the bacterial liquid, and uniformly mixing in a test tube containing 9.0ml of sterile physiological saline, wherein the dilution is 10 -1 Repeating the above steps to sequentially obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Is a bacterial liquid of (a) a strain. Selection 10 -4 、10 -5 、10 -6 Three dilutions, each of which was aspirated 0.1ml of bacterial fluid, were separately dropped onto MRS medium plates (Beijing oblongsu) under anaerobic conditionsCulturing at 37 deg.c for 24-48 hr.
(1) Acid and bile salt resistant growth performance screening test:
separating to obtain 39 strains, respectively activating the strains, inoculating to MRS liquid culture medium, culturing for 24 hr, centrifuging 3ml of fermentation liquid at 3000rpm for 5 min, adding into 3ml of liquid culture medium containing 0.1% bile salt pH=3, incubating for 2 hr, inoculating to fresh culture medium at 10%, incubating for 4 hr, and measuring OD 600nm The acid and bile salt resistance of the strain was observed, and the results are shown in Table 1 below.
The strain with excellent growth performance and acid and bile salt resistance is obtained, the survival rate of ZWC030 is 83.86% under the condition of 0.1% bile salt pH=3.0, and the strain grows best, so that ZWC030 is screened as the optimal strain.
TABLE 1 results of lactic acid bacteria screening test
(2) Identification of Excellent strains
The strain ZWC030 is screened and sent to the China general microbiological culture Collection center for identification from two aspects of physiological biochemistry and molecular biology. The primers for molecular biology PCR amplification (27F, 149 2R) are respectively: 27F (SEQ ID NO.1: 5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (SEQ ID NO.2: 5'-TACGACTTAACCCCAATCGC-3').
The colony morphology of the optimal strain is specifically observed in the figures 1 and 2, physiological and biochemical characteristic analysis and 16SrDNA molecular identification are carried out, the results are shown in the following table 2 and SEQ ID NO.3, and the strain ZWC030 is Weissella food (Weissella cibaria).
TABLE 2 physiological and biochemical characteristics of ZWC030 Strain
SEQ ID NO.3:
gctccgaaggttaccccaccggctttgggtgttacaaactctcatggtgtgacgggcggtgtgtacaagacccgggaacgtattcaccgcggcgtgctgatccgcgattactagcgattccgacttcatgtaggcgagttgcagcctacaatccgaactgagacgtactttaagagattagctcaccctcgcgggttggcaactcgttgtatacgccattgtagcacgtgtgtagcccaggtcataaggggcatgatgatttgacgtcatccccaccttcctccggtttgtcaccggcagtctcactagagtgcccaactgaatgctggcaactagtaataagggttgcgctcgttgcgggacttaacccaacatctcacgacacgagctgacgacaaccatgcaccacctgtcaccttgtccccgaagggaacgctccatctctggagttgtcaagggatgtcaagacctggtaaggttcttcgcgttgcttcgaattaaaccacatgctccaccgcttgtgcgggtccccgtcaattcctttgagtttcaaccttgcggtcgtactccccaggcggagtgcttaatgcgttagctgcggcacttaagggcggaaaccctcaaacacctagcactcatcgtttacggtgtggactaccagggtatctaatcctgtttgctacccacactttcgagcctcaacgtcagttacagtccagaaagccgccttcgccactggtgttcttccatatatctacgcaccgctacacatggacttccactttcctctactgcactcaagtcatccagtttccaaagcaattcctcagttgagctgagggctttcacttcagacttaaataaccgtctgcgctcgctttacgcccaataaatccggataacgcttggaacatacgtattaccgcggctgctggcacgtatttagccgttcctttctggtaagataccgtcacacattgaacagttactctcaatgtcattcttctcttacaacagtgttttacgagccgaaacccttcatcacacacgcggcgttgctccatcaggctttcgcccattgtggaagattccctactgctgcctcccgtaggagtatgggccgtgtctcagtcccattgtggccgatcagtctctcaactcggctatgcatcatcgtcttggtgagccattacctcaccaactaactaatgcaccgcgggaccatctcttagtgatagcagaaccatcttttaagtagcaaccatgcggttgctattgttatacggtattagcatctgtttccaaatgttatcccctgctaagaggtaggtttcccacgtgttactcacccgttcgccactctttgcaatgtccatcgtcatatctgagcaagctcttcaaatcagtt
The strain ZWC was sent to China general microbiological culture Collection center (address: national institute of microbiology, national academy of sciences of China, postal code: 100101) for patent preservation, accession number: CGMCC No.26854, preserving strain: ZWC030, class naming: weissella antrum Weissella cibaria.
2. Weissella antrum growth curve
After activation of Weissella antrum ZWC030, the cells were inoculated into MRS liquid medium (Beijing Oibuping) at an inoculum size of 0.5% (v/v), 1% (v/v), 2% (v/v), 3% (v/v) and 5% (v/v), respectively, and were allowed to stand at 37℃for 48 hours. The OD600nm value of the bacterial liquid of 0-48 h is measured by a full-automatic growth curve analyzer Bioscreen C (Shanghai, ministry of bearing technologies, inc.), and the growth curve is drawn, as shown in FIG. 3, and the growth performance of the strain is best when the inoculation amount is 5%.
Example 2 Weissella food ZWC030 in vitro deodorization test
1. Nitrogen migration law analysis
NH is added into fresh pig manure liquid 4 Cl is taken as a nitrogen source of a culture medium, liquid culture medium, weissella ZWC030 and lactobacillus plantarum are respectively inoculated, the inoculation amount is 10%, the culture is carried out for 24 hours at 37 ℃, the nitrogen source utilization condition of different strains is determined, and the control group is fermentation broth without inoculating lactobacillus strains. The results are shown in Table 3.
TABLE 3 analysis of nitrogen migration law results
The results show that: utilization of NH with strains 4 Cl results show that the conversion rate of ammonium nitrogen to amide nitrogen is up to 81.3% by Weissella antrum ZWC030, and the ammonium nitrogen is ionic NH 4+ N, ammonium Nitrogen (NH) in dissolved form 4+ -N) conversion to dissolved ammonia (NH) 3 ) Finally with gaseous ammonia (NH) 3 ) The high content of ammonium nitrogen indicates that the nitrogen is volatilized and discharged to the environment. The results indicate that Weissella food reduces nitrogen emissions to the environment.
2. Weissella antrum ZWC030 in vitro affecting fecal metabonomics analysis
In vitro, 10% of Weissella antrum ZWC030 was inoculated into pig manure, accumulation was carried out at room temperature, metabolomics was measured on days 3 and 5, and the control group was a group not inoculated with Weissella antrum, as shown in FIG. 4, in which: CON3 was day 1, CON5 was day 5, LM3 was day 3, and LM5 was day 5. L-glutamic Acid, L-Proline, L-Proline, piperidinyl, 7 alpha-Hydroxy-3-oxo-5beta-cholan-24-oic Acid, 7alpha-Hydroxy-3-oxo-5β -chloro-24 Acid, simonin IV, 3- (4-Hydroxyphenyl) lactate, 3- (4-Hydroxyphenyl) lactic Acid, sulfate, leucinic Acid, flash Acid, M-communication Acid, meta-coumarin Acid, 6-Hydroxyhexanoic Acid, 6-hydroxycaproic Acid, N-Methyl-a-aminoisobutyric caid, N-Methyl-aminoisobutyric Acid, phenyllactic Acid, phenyllactic Acid, deoxycholic Acid, 6,8-Dihydroxypurine, xanthine Xanthine, 5-Aminopentanoic Acid, cinnoline, noraminovaleric Acid, and norbornane. The results showed that the amino acid products such as L-glutamate, 6-hydroxycaproic acid, P-phenylalanine increased with increasing accumulation time compared to the control group, but the rate of increase of these metabolites was lower after treatment with Weissella fraxini than the control group, indicating that the strain affected malodorous substances production by controlling fecal microbial metabolites.
EXAMPLE 3 deodorizing Effect of fermented feed prepared from Weissella antrum ZWC030 in production of growing pigs and application
Preparation of fermented feed of sinus Weissella ZWC030 screened in example 1: mixing whole pig feed (corn-bean pulp, drinking water at a ratio of 1:1.2 by weight), adding 0.30% of Weissella antrum ZWC030 by weight, mixing, packaging into clean stainless steel barrel (or plastic barrel), compacting, sealing, placing the barrel into a closed culture device, culturing at 37deg.C for 30 hr, and unsealing to obtain fermented feed.
Selecting 60 healthy growing pigs, randomly dividing the pigs into 2 groups, wherein each group comprises 3 repeats and 10 repeats, feeding basic ration to a control group pig group, and adding 1% of the prepared fermented feed to the basic ration by a test group pig group. Continuously feeding for 35 days, and allowing the swinery to eat and drink water freely for routine immunization. Other feeding management and immunization procedures are uniformly carried out according to a large-scale pig farm.
1. Production performance
At the beginning and end of the test, individuals weighed on an empty stomach, the growth of the pig group was observed daily, the feed consumption was recorded, and after the end of the test, the daily gain, daily feed intake and feed weight ratio were calculated, and the results are shown in Table 4.
Compared with the control group, the average daily gain of the pig group in the test group is increased by 8.02 percent (P < 0.05), the feed weight ratio is reduced by 3.83 percent (P < 0.05), and the feed weight ratio=average daily feed intake/average daily gain.
TABLE 4 analysis of growing pig production Performance results
| Project | Control group | Test group |
| Initial weight of test kg | 30.38±9.96 | 32.35±11.25 |
| End of test kg | 57.32±14.95 b | 60.12±15.85 a |
| Average daily gain kg/d | 0.735±0.110 b | 0.794±0.086 a |
| Daily average feed intake kg | 1.815±0.10 | 1.799±0.14 |
| Ratio of material to weight | 2.35±0.028 a | 2.26±0.014 b |
Note that: the same expression difference of the same-row digital shoulder marks and lowercase letters is not obvious [ ]P>0.05 Small letters are different to indicate significant differenceP<0.05). Data in the table are mean ± standard deviation. The table below is the same.
2 analysis of fecal odor
Measuring odor change in the feces accumulation process by using a sensor, weighing 35 g of feces of pigs after continuous feeding for 35 days (collecting multiple pigs for uniform mixing), hermetically storing in 500mL blue-cap bottles, and measuring gas CO by using the sensor on days 1, 2, 3, 4, 5, 6 and 7 respectively 2 、H 2 S、NH 3 See fig. 5, 6, 7 for results. The results indicate that the test group inhibited H 2 S、NH 3 The effect of (2) is more obvious. H compared with the control group 2 S can be reduced by more than 41% in days 1, 2, 3, 4, 5, 6 and 7 respectively, NH 3 No significant effect is found on days 1 and 2, and the reduction of H is more than 60% on days 3, 4, 5, 6 and 7 2 S、NH 3 Is a main source for generating odor, and can effectively reduce the odor when animals are fed with the fermented feed of the antral Weissella ZWC 030.
Claims (6)
1. Weissella of food sinusWeissella cibaria) ZWC030, which is deposited with the China general microbiological culture Collection center, having a deposit number of: CGMCC No.26854.
2. The method according to claim 1Weissella cibaria) Use of ZWC030 in the manufacture of an animal feed deodorant.
3. A fermentation additive for reducing animal breeding odor, which is prepared from the Weissella deliciosa of claim 1Weissella cibaria) ZWC030 fermented complete feed。
4. A fermentation additive according to claim 3, wherein the antral weissella spWeissella cibaria) The inoculation amount of ZWC030 in the complete feed is 0.3-0.5% of the weight of the complete feed.
5. A fermentation additive according to claim 3, wherein the amount of fermentation additive added to the feed is 0.8-3% by weight of the feed.
6. A feed for reducing animal breeding odor, comprising the Weissella food of claim 1Weissella cibaria）ZWC030。
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