CN117228831A - Preparation method of composite carbon source for removing nitrogen in denitrification tank - Google Patents
Preparation method of composite carbon source for removing nitrogen in denitrification tank Download PDFInfo
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- CN117228831A CN117228831A CN202311506408.1A CN202311506408A CN117228831A CN 117228831 A CN117228831 A CN 117228831A CN 202311506408 A CN202311506408 A CN 202311506408A CN 117228831 A CN117228831 A CN 117228831A
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 71
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 71
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 239000002131 composite material Substances 0.000 title claims abstract description 49
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 claims abstract description 139
- 229920000903 polyhydroxyalkanoate Polymers 0.000 claims abstract description 139
- 241000209140 Triticum Species 0.000 claims abstract description 60
- 235000021307 Triticum Nutrition 0.000 claims abstract description 60
- 239000010902 straw Substances 0.000 claims abstract description 60
- 239000000843 powder Substances 0.000 claims abstract description 56
- 238000002156 mixing Methods 0.000 claims abstract description 40
- 239000002245 particle Substances 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 26
- 229920002261 Corn starch Polymers 0.000 claims abstract description 18
- 239000008120 corn starch Substances 0.000 claims abstract description 18
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 235000011837 pasties Nutrition 0.000 claims abstract description 10
- 230000008569 process Effects 0.000 claims abstract description 7
- 239000000853 adhesive Substances 0.000 claims abstract description 5
- 230000001070 adhesive effect Effects 0.000 claims abstract description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 33
- 238000004880 explosion Methods 0.000 claims description 21
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- 239000003960 organic solvent Substances 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 8
- 238000001125 extrusion Methods 0.000 claims description 8
- 239000012535 impurity Substances 0.000 claims description 8
- 230000000379 polymerizing effect Effects 0.000 claims description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 6
- 229920002488 Hemicellulose Polymers 0.000 claims description 6
- 239000001913 cellulose Substances 0.000 claims description 6
- 229920002678 cellulose Polymers 0.000 claims description 6
- 229920005610 lignin Polymers 0.000 claims description 6
- 238000007605 air drying Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 238000001694 spray drying Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 239000010865 sewage Substances 0.000 abstract description 7
- 239000006227 byproduct Substances 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 239000003054 catalyst Substances 0.000 abstract 1
- 238000007781 pre-processing Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 7
- 239000010802 sludge Substances 0.000 description 7
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 5
- 239000001632 sodium acetate Substances 0.000 description 5
- 235000017281 sodium acetate Nutrition 0.000 description 5
- 230000006978 adaptation Effects 0.000 description 4
- 238000007792 addition Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- VGPSUIRIPDYGFV-UHFFFAOYSA-N [N].O[N+]([O-])=O Chemical compound [N].O[N+]([O-])=O VGPSUIRIPDYGFV-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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Abstract
The invention provides a preparation method of a composite carbon source for removing nitrogen in a denitrification tank, belongs to the field of sewage treatment, and solves the problems of danger, high price and the like of externally-thrown carbon sources. The invention comprises the following steps: s1, preprocessing wheat straw; s2, polyhydroxyalkanoate PHA pretreatment: s3, mixing: selecting corn starch solution as an adhesive, and placing the pretreated wheat straw powder and PHA powder into mixing equipment for mixing to form a pasty mixture; s4, forming composite carbon source particles: converting the pasty mixture into composite carbon source particles by adopting granulating equipment; s5, storing. According to the invention, wheat straw is used as a waste agricultural byproduct, and is converted into carbon source powder through pretreatment, crushing and other steps, so that comprehensive utilization of the wheat straw is realized. The three components are adopted, and the proportion of different components is adjusted to flexibly apply the catalyst under different conditions, so that different requirements in the denitrification process are met. And has the advantages of low cost and low risk.
Description
Technical Field
The invention belongs to the field of sewage treatment, and relates to an additive preparation in a denitrification tank, in particular to a preparation method of a composite carbon source for removing nitrogen in the denitrification tank.
Background
In town sewage treatment, the actual inflow COD is generally low, the requirement of denitrification on a carbon source in the treatment process cannot be met, and the additional carbon source is required to be added to improve the water quality C/N so as to ensure that biological denitrification in a treatment system is normally carried out and achieve the aim of denitrification, thereby ensuring that the total nitrogen index of the effluent reaches the standard and is discharged normally.
The main external carbon source in the current market has the following characteristics:
methanol as an external carbon source has the advantages of low running cost and small sludge yield, but has the following problems to be paid attention to:
1. methanol is inflammable and is a class A hazardous chemical substance, and has strict requirements for storage and use;
2. the response time of the microorganism to the methanol is slow, the methanol can not be utilized by all microorganisms, and the effect is poor when the methanol is used for the emergency carbon source addition of a sewage treatment plant;
3. methanol has certain toxic action, and takes methanol as a long-term carbon source, and can also have certain influence on tail water discharge.
The following three points are considered for sodium acetate:
1. most of sodium acetate is 20%, 25% and 30% liquid, and the sodium acetate cannot be transported for a long distance due to low equivalent COD and high transportation cost;
2. the sludge yield is large, so that the sludge treatment cost is increased;
3. the price is relatively high, and the large-scale addition of sodium acetate in a sewage treatment plant is almost impossible.
In summary, it is desirable to provide a new carbon source to meet the demand of denitrification for carbon sources.
Disclosure of Invention
The invention aims at solving the problems in the prior art and provides a preparation method of a composite carbon source for removing nitrogen in a denitrification tank.
The aim of the invention can be achieved by the following technical scheme: the preparation method of the composite carbon source for removing nitrogen in the denitrification tank comprises the following steps of:
s1, pretreatment of wheat straw:
cleaning wheat straw to remove soil and impurities, drying the wheat straw to a moisture content lower than 10%, crushing the wheat straw into powder, and treating the wheat straw powder by a steam explosion method;
s2, polyhydroxyalkanoate PHA pretreatment:
s2.1, using sucrose and bacillus as raw materials, fermenting and polymerizing the sucrose into polyhydroxyalkanoate PHA by using the bacillus at the temperature ranging from 25 ℃ to 37 ℃ and the pH value ranging from 6.5 to 7.5;
s2.2, polymerizing to generate polyhydroxyalkanoate PHA solution, dropwise adding an organic solvent into the polyhydroxyalkanoate PHA solution for washing and extracting, and removing unreacted sucrose, bacillus and impurities;
s2.3, placing the purified polyhydroxyalkanoate PHA solution in an evaporator, heating and utilizing high temperature to evaporate the organic solvent from the polyhydroxyalkanoate PHA solution, and concentrating the residual polyhydroxyalkanoate PHA solution to a required concentration;
s2.4, spray drying or solidifying the polyhydroxyalkanoate PHA solution with high concentration to generate PHA particles; adding PHA particles into a grinder to prepare polyhydroxyalkanoate PHA powder;
s3, mixing:
selecting corn starch solution as an adhesive, and placing the pretreated wheat straw powder and polyhydroxyalkanoate PHA powder into stirring equipment for stirring and mixing to form a pasty mixture;
s4, forming composite carbon source particles:
converting the pasty mixture into composite carbon source particles by adopting granulating equipment;
s5, storing:
and storing the composite carbon source particles in a sealed container for later use.
In the above preparation method of the composite carbon source for removing nitrogen in the denitrification tank, in step S1, the components of the wheat straw are measured by dry weight, and the method comprises the following steps: cellulose at a ratio of 42% to 50%, hemicellulose at a ratio of 30% to 40%, lignin at a ratio of 20% to 25%.
In the preparation method of the composite carbon source for removing nitrogen in the denitrification tank, in the step S1, wheat straw is dried in a natural sun-drying and air-drying mode; grinding wheat straw into powder with the size of 300-355 microns through a 9FQ series hammer grinder, and screening through a 50-mesh screen; the steam explosion method adopts steam explosion equipment, the steam explosion temperature is controlled to be 180 ℃ to 220 ℃, the steam explosion pressure is controlled to be 1MPa to 1.5MPa, and the steam explosion time is controlled to be 30-60 minutes.
In the above preparation method of the composite carbon source for nitrogen removal in the denitrification tank, in step S2.2, the organic solvent is specifically at least one of chloroform, methanol, ethanol and dimethyl ketone.
In the above method for preparing a composite carbon source for nitrogen removal in a denitrification tank, in step S2.4, polyhydroxyalkanoate PHA powder has a size of 150-200 μm.
In the preparation method of the composite carbon source for removing nitrogen in the denitrification tank, in the step S3, the concentration of the corn starch solution is 7-10%, and 40-50 g of the corn starch solution is added per 100 g of the mixture of the wheat straw powder and the polyhydroxyalkanoate PHA powder.
In the preparation method of the composite carbon source for removing nitrogen in the denitrification tank, in the step S3, the weight ratio of the mixture of the wheat straw powder and the polyhydroxyalkanoate PHA powder is as follows: 1:1-2:1, a step of; the stirring device adopts ROSS High Shear Mixers series high-speed mixing device, the mixing speed is controlled between 500 rpm and 2000rpm, and the mixing time is controlled between 10 minutes and 30 minutes.
In the preparation method of the composite carbon source for removing nitrogen in the denitrification tank, in the step S3, the mixing steps are as follows: firstly, putting wheat straw powder and polyhydroxyalkanoate PHA powder into stirring equipment, gradually adding corn starch solution, and uniformly stirring in the mixing process.
In the preparation method of the composite carbon source for removing nitrogen in the denitrification tank, in the step S3, granulating equipment adopts a California Pellet Mill 7900 series low-speed extrusion granulator, and the granulating temperature of the low-speed extrusion granulator is controlled between 60 ℃ and 80 ℃ so as to maintain the structural integrity of polyhydroxyalkanoate PHA; the diameter of the prepared composite carbon source particles is 2-3 mm.
Compared with the prior art, the preparation method of the composite carbon source for removing nitrogen in the denitrification tank has the following beneficial effects:
1. comprehensively utilizing agricultural and sideline products: the method utilizes the wheat straw as a waste agricultural byproduct, converts the wheat straw into powder which can be used as a carbon source through the steps of pretreatment, crushing and the like, and realizes the comprehensive utilization of the wheat straw.
2. Multicomponent composite carbon source: the composite carbon source consists of three components of wheat straw, polyhydroxyalkanoate PHA and corn starch, and can be flexibly applied under different conditions by adjusting the proportion of different components, so as to meet different requirements in the denitrification process.
3. Low cost, low risk: compared with the traditional additional carbon sources such as methanol, sodium acetate and the like, the preparation method of the composite carbon source has the advantages of low cost, and relatively safe and simple storage and use.
4. Controllability and stability: the prepared composite carbon source particles have better controllability and stability through the steps of crushing, mixing, granulating and the like, and are convenient to store and use.
Detailed Description
The following are specific examples of the present invention, and the technical solutions of the present invention are further described, but the present invention is not limited to these examples.
First embodiment:
a preparation method of a composite carbon source for removing nitrogen in a denitrification tank comprises the following steps:
s1, pretreatment of wheat straw: cleaning wheat straw to remove soil and impurities, drying the wheat straw to a moisture content lower than 10%, crushing the wheat straw into powder, and treating the wheat straw powder by a steam explosion method;
s2, polyhydroxyalkanoate PHA pretreatment:
s2.1, using sucrose and bacillus as raw materials, fermenting and polymerizing the sucrose into polyhydroxyalkanoate PHA by using the bacillus at the temperature ranging from 25 ℃ to 37 ℃ and the pH value ranging from 6.5 to 7.5;
s2.2, polymerizing to generate polyhydroxyalkanoate PHA solution, dropwise adding an organic solvent into the polyhydroxyalkanoate PHA solution for washing and extracting, and removing unreacted sucrose, bacillus and impurities; the organic solvent is at least one of chloroform, methanol, ethanol and dimethyl ketone.
S2.3, placing the purified polyhydroxyalkanoate PHA solution in an evaporator, heating and utilizing high temperature to evaporate the organic solvent from the polyhydroxyalkanoate PHA solution, and concentrating the residual polyhydroxyalkanoate PHA solution to a required concentration;
s2.4, spray drying or solidifying the polyhydroxyalkanoate PHA solution with high concentration to generate PHA particles; adding PHA particles into a grinder to prepare polyhydroxyalkanoate PHA powder;
s3, mixing:
selecting corn starch solution as an adhesive, and placing the pretreated wheat straw powder and polyhydroxyalkanoate PHA powder into mixing equipment for mixing to form a pasty mixture;
s4, forming composite carbon source particles: converting the pasty mixture into composite carbon source particles by adopting granulating equipment;
s5, storing: and storing the composite carbon source particles in a sealed container for later use.
Preferably, the components of the wheat straw, on a dry weight basis, comprise: 45% cellulose, 33% hemicellulose and 22% lignin;
preferably, in the step S1, the wheat straw is dried to a moisture content lower than 10% by adopting a natural sun-drying and air-drying mode;
grinding wheat straw into powder with the size of 300 microns through a 9FQ series hammer grinder, and screening through a 50-mesh screen;
the steam explosion method adopts an SPB-500 steam explosion reactor, the temperature is controlled between 180 ℃ and 220 ℃, the pressure is controlled between 1MPa and 1.5MPa, and the steam explosion time is controlled to 40 minutes;
preferably, polyhydroxyalkanoate PHA powder size is 180 microns;
the mixing proportion of the wheat straw powder and the polyhydroxyalkanoate PHA powder is as follows: 1:1 (weight ratio);
the mixing equipment adopts ROSS High Shear Mixers series high-speed mixing equipment, the mixing speed is controlled between 500 rpm and 2000rpm, and the mixing time is controlled between 20 minutes and 25 minutes;
the concentration of the corn starch solution is 8%, and the adding amount of the corn starch solution is 45 g per 100 g of the mixture of the wheat straw powder and the polyhydroxyalkanoate PHA powder;
the mixing steps are as follows: firstly, putting wheat straw powder and polyhydroxyalkanoate PHA powder into mixing equipment, gradually adding corn starch solution, and ensuring that uniform stirring is kept in the mixing process;
preferably, in step S3,
the granulating equipment adopts a California Pellet Mill 7900 series low-speed extrusion granulator, and the granulating temperature of the low-speed extrusion granulator is controlled between 60 ℃ and 80 ℃ so as to maintain the structural integrity of the polyhydroxyalkanoate PHA;
the particle size of the composite carbon source is 2 mm.
Adding 2g of composite carbon source into activated sludge with the concentration of 2500mg/L and the volume of 20ml, and after the activated sludge is placed into 500ml of sewage with the concentration of nitric acid nitrogen of 50mg/L, the temperature of 25 ℃ and the ph value of 7.8 and the concentration of aerobic denitrification bacterial liquid of 5g/L, the hydraulic retention time is 24 hours, obtaining the following data through multiple experiments:
2 hours: the initial denitrification rate is slow due to the gradual release of the carbon source and the gradual adaptation of denitrifying microorganisms, and the denitrification removal rate is 10-15%;
4 hours: along with the release of the carbon source and the gradual adaptation of microorganisms, the denitrification rate is increased, and the denitrification removal rate is 33.2-40.5%;
6 hours: the denitrification removal rate is continuously increased to 45-54.8%;
8 hours: the denitrification removal rate reaches 61.3-68.5%;
10 hours: the denitrification removal rate reaches 71.3-79.6%;
12 hours: the denitrification removal rate reaches 75.4-85.1%;
24 hours: the denitrification removal rate reaches 90-93.4%;
between 8-10 hours, the total nitrogen concentration can be reduced to approximately 15 mg/l or less, meeting the nitrogen emission standards.
Specific embodiment II:
a preparation method of a composite carbon source for removing nitrogen in a denitrification tank comprises the following steps:
s1, pretreatment of wheat straw: cleaning wheat straw to remove soil and impurities, drying the wheat straw to a moisture content lower than 10%, crushing the wheat straw into powder, and treating the wheat straw powder by a steam explosion method;
s2, polyhydroxyalkanoate PHA pretreatment:
s2.1, using sucrose and bacillus as raw materials, fermenting and polymerizing the sucrose into polyhydroxyalkanoate PHA by using the bacillus at the temperature ranging from 25 ℃ to 37 ℃ and the pH value ranging from 6.5 to 7.5;
s2.2, polymerizing to generate polyhydroxyalkanoate PHA solution, dropwise adding an organic solvent into the polyhydroxyalkanoate PHA solution for washing and extracting, and removing unreacted sucrose, bacillus and impurities;
s2.3, placing the purified polyhydroxyalkanoate PHA solution in an evaporator, heating and utilizing high temperature to evaporate the organic solvent from the polyhydroxyalkanoate PHA solution, and concentrating the residual polyhydroxyalkanoate PHA solution to a required concentration;
s2.4, spray drying or solidifying the polyhydroxyalkanoate PHA solution with high concentration to generate PHA particles; adding PHA particles into a grinder to prepare polyhydroxyalkanoate PHA powder;
s3, mixing:
selecting corn starch solution as an adhesive, and placing the pretreated wheat straw powder and polyhydroxyalkanoate PHA powder into mixing equipment for mixing to form a pasty mixture;
s4, forming composite carbon source particles: converting the pasty mixture into composite carbon source particles by adopting granulating equipment;
s5, storing: and storing the composite carbon source particles in a sealed container for later use.
Preferably, the components of the wheat straw, on a dry weight basis, comprise: 45% cellulose, 33% hemicellulose and 22% lignin;
preferably, in the step S1, the wheat straw is dried to a moisture content lower than 10% by adopting a natural sun-drying and air-drying mode;
grinding wheat straw into powder with the size of 300 microns through a 9FQ series hammer grinder, and screening through a 50-mesh screen;
the steam explosion method adopts an SPB-500 steam explosion reactor, the temperature is controlled between 180 ℃ and 220 ℃, the pressure is controlled between 1MPa and 1.5MPa, and the steam explosion time is controlled to be 50 minutes;
preferably, polyhydroxyalkanoate PHA powder size is 180 microns;
the mixing proportion of the wheat straw powder and the polyhydroxyalkanoate PHA powder is as follows: 2:1 (weight ratio);
the mixing equipment adopts ROSS High Shear Mixers series high-speed mixing equipment, the mixing speed is controlled between 500 rpm and 2000rpm, and the mixing time is controlled between 25 minutes and 30 minutes;
the concentration of the corn starch solution is 8%, and 50 g of corn starch solution is added to 100 g of the mixture of the wheat straw powder and the polyhydroxyalkanoate PHA powder;
the mixing steps are as follows: firstly, putting wheat straw powder and polyhydroxyalkanoate PHA powder into mixing equipment, gradually adding corn starch solution, and ensuring that uniform stirring is kept in the mixing process;
preferably, in step S3,
the granulating equipment adopts a California Pellet Mill 7900 series low-speed extrusion granulator, and the granulating temperature of the low-speed extrusion granulator is controlled between 60 ℃ and 80 ℃ so as to maintain the structural integrity of the polyhydroxyalkanoate PHA;
the particle size of the composite carbon source is 3 mm.
Adding 2g of composite carbon source into activated sludge with the concentration of 2500mg/L and the volume of 20ml, and after the activated sludge is placed into 500ml of sewage with the concentration of nitric acid nitrogen of 50mg/L, the temperature of 25 ℃ and the ph value of 7.8 and the concentration of aerobic denitrification bacterial liquid of 5g/L, the hydraulic retention time is 24 hours, obtaining the following data through multiple experiments:
2 hours: the initial denitrification rate is slow due to the gradual release of the carbon source and the gradual adaptation of denitrifying microorganisms, and the denitrification removal rate is 10-15%;
4 hours: along with the release of the carbon source and the gradual adaptation of microorganisms, the denitrification rate is increased, and the denitrification removal rate is 30-39.2%;
6 hours: the removal rate is continuously increased, and the denitrification removal rate reaches 43.4-54.8%;
8 hours: the denitrification removal rate reaches 57.2-64.5%;
10 hours: the denitrification removal rate reaches 68.2-75.3%;
12 hours: the denitrification removal rate reaches 73.1-85%;
24 hours: the denitrification removal rate reaches 90-93.1%;
between 10 and 12 hours, the total nitrogen concentration can be reduced to approximately 15 mg/l or less, meeting the nitrogen emission standards.
The principle of adopting wheat straw and polyhydroxyalkanoate PHA as a composite carbon source is as follows:
cellulose, hemicellulose and lignin which take wheat straw as a carbon source are more easily degraded by microorganisms after steam explosion, polyhydroxyalkanoate PHA provides a carbon source which is easier to degrade, the wheat straw is utilized to provide mechanical strength and support for a composite carbon source, and the combination of the cellulose, the hemicellulose and the lignin can accelerate the degradation of the carbon source and improve the treatment speed;
wheat straw is relatively cheap, and polyhydroxyalkanoate PHA can be used for improving the performance of a carbon source, so that the treatment cost can be reduced.
The specific embodiments described herein are offered by way of example only to illustrate the spirit of the invention. Those skilled in the art may make various modifications or additions to the described embodiments or substitutions thereof without departing from the spirit of the invention or exceeding the scope of the invention as defined.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Claims (9)
1. The preparation method of the composite carbon source for removing nitrogen in the denitrification tank is characterized by comprising the following steps of:
s1, pretreatment of wheat straw:
cleaning wheat straw to remove soil and impurities, drying the wheat straw to a moisture content lower than 10%, crushing the wheat straw into powder, and treating the wheat straw powder by a steam explosion method;
s2, polyhydroxyalkanoate PHA pretreatment:
s2.1, using sucrose and bacillus as raw materials, fermenting and polymerizing the sucrose into polyhydroxyalkanoate PHA by using the bacillus at the temperature ranging from 25 ℃ to 37 ℃ and the pH value ranging from 6.5 to 7.5;
s2.2, polymerizing to generate polyhydroxyalkanoate PHA solution, dropwise adding an organic solvent into the polyhydroxyalkanoate PHA solution for washing and extracting, and removing unreacted sucrose, bacillus and impurities;
s2.3, placing the purified polyhydroxyalkanoate PHA solution in an evaporator, heating and utilizing high temperature to evaporate the organic solvent from the polyhydroxyalkanoate PHA solution, and concentrating the residual polyhydroxyalkanoate PHA solution to a required concentration;
s2.4, spray drying or solidifying the polyhydroxyalkanoate PHA solution with high concentration to generate PHA particles; adding PHA particles into a grinder to prepare polyhydroxyalkanoate PHA powder;
s3, mixing:
selecting corn starch solution as an adhesive, and placing the pretreated wheat straw powder and polyhydroxyalkanoate PHA powder into stirring equipment for stirring and mixing to form a pasty mixture;
s4, forming composite carbon source particles:
converting the pasty mixture into composite carbon source particles by adopting granulating equipment;
s5, storing:
and storing the composite carbon source particles in a sealed container for later use.
2. The method for preparing a nitrogen-depleted composite carbon source in a denitrification tank according to claim 1, wherein in step S1, the components of the wheat straw are measured in dry weight, comprising: cellulose at a ratio of 42% to 50%, hemicellulose at a ratio of 30% to 40%, lignin at a ratio of 20% to 25%.
3. The method for preparing a nitrogen-removed composite carbon source in a denitrification tank according to claim 1, wherein in step S1, wheat straw is dried by natural sun-drying and air-drying; grinding wheat straw into powder with the size of 300-355 microns through a 9FQ series hammer grinder, and screening through a 50-mesh screen; the steam explosion method adopts steam explosion equipment, the steam explosion temperature is controlled to be 180 ℃ to 220 ℃, the steam explosion pressure is controlled to be 1MPa to 1.5MPa, and the steam explosion time is controlled to be 30-60 minutes.
4. The method for preparing a nitrogen-removing composite carbon source in a denitrification tank according to claim 1, wherein in step S2.2, the organic solvent is at least one of chloroform, methanol, ethanol and dimethyl ketone.
5. The method of claim 1, wherein in step S2.4, the polyhydroxyalkanoate PHA powder has a size of 150-200 microns.
6. The method for preparing a nitrogen-depleted complex carbon source in a denitrification tank according to claim 1, wherein in step S3, the concentration of the corn starch solution is 7-10%, and 40-50 g of the corn starch solution is added per 100 g of the mixture of wheat straw powder and polyhydroxyalkanoate PHA powder.
7. The method for preparing a nitrogen-depleted complex carbon source in a denitrification tank according to claim 1, wherein in step S3, the weight ratio of the wheat straw powder to the polyhydroxyalkanoate PHA powder is: 1:1-2:1, a step of; the stirring device adopts ROSS High Shear Mixers series high-speed mixing device, the mixing speed is controlled between 500 rpm and 2000rpm, and the mixing time is controlled between 10 minutes and 30 minutes.
8. The method for preparing a nitrogen-removing composite carbon source in a denitrification tank according to claim 1, wherein in step S3, the mixing step is as follows: firstly, putting wheat straw powder and polyhydroxyalkanoate PHA powder into stirring equipment, gradually adding corn starch solution, and uniformly stirring in the mixing process.
9. The method for preparing a nitrogen-depleted composite carbon source in a denitrification tank according to claim 1, wherein in step S3, granulating equipment adopts a California Pellet Mill 7900 series low-speed extrusion granulator, and the granulating temperature of the low-speed extrusion granulator is controlled between 60 ℃ and 80 ℃ so as to maintain the structural integrity of polyhydroxyalkanoate PHA; the diameter of the prepared composite carbon source particles is 2-3 mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202311506408.1A CN117228831A (en) | 2023-11-14 | 2023-11-14 | Preparation method of composite carbon source for removing nitrogen in denitrification tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311506408.1A CN117228831A (en) | 2023-11-14 | 2023-11-14 | Preparation method of composite carbon source for removing nitrogen in denitrification tank |
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