CN117225336A - Amino acid synthesis equipment and method - Google Patents
Amino acid synthesis equipment and method Download PDFInfo
- Publication number
- CN117225336A CN117225336A CN202311499489.7A CN202311499489A CN117225336A CN 117225336 A CN117225336 A CN 117225336A CN 202311499489 A CN202311499489 A CN 202311499489A CN 117225336 A CN117225336 A CN 117225336A
- Authority
- CN
- China
- Prior art keywords
- water
- amino acid
- discharge reaction
- reaction cavity
- mixed gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 94
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 29
- 238000003786 synthesis reaction Methods 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 112
- 238000006243 chemical reaction Methods 0.000 claims abstract description 79
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000007789 gas Substances 0.000 claims abstract description 47
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 38
- 150000005839 radical cations Chemical class 0.000 claims abstract description 36
- 238000003860 storage Methods 0.000 claims abstract description 31
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 26
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 26
- 239000000126 substance Substances 0.000 claims abstract description 24
- 238000001308 synthesis method Methods 0.000 claims abstract description 17
- JCXJVPUVTGWSNB-UHFFFAOYSA-N nitrogen dioxide Inorganic materials O=[N]=O JCXJVPUVTGWSNB-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000010438 heat treatment Methods 0.000 claims abstract description 12
- 235000001014 amino acid Nutrition 0.000 claims description 89
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 18
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 16
- 229910052757 nitrogen Inorganic materials 0.000 claims description 11
- 239000004471 Glycine Substances 0.000 claims description 9
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 9
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 9
- 235000004279 alanine Nutrition 0.000 claims description 9
- 235000003704 aspartic acid Nutrition 0.000 claims description 9
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 9
- 150000001732 carboxylic acid derivatives Chemical class 0.000 claims description 9
- 229910021529 ammonia Inorganic materials 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 239000002250 absorbent Substances 0.000 claims description 3
- 230000002745 absorbent Effects 0.000 claims description 3
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical group C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- 239000003054 catalyst Substances 0.000 abstract description 7
- 239000000523 sample Substances 0.000 description 15
- 239000000376 reactant Substances 0.000 description 9
- 150000002500 ions Chemical class 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 238000001819 mass spectrum Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000004949 mass spectrometry Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229910001873 dinitrogen Inorganic materials 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000005725 8-Hydroxyquinoline Substances 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- WEVYAHXRMPXWCK-UHFFFAOYSA-N acetonitrile Substances CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006664 bond formation reaction Methods 0.000 description 1
- 150000001721 carbon Chemical group 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000001212 derivatisation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 229960003540 oxyquinoline Drugs 0.000 description 1
- ZWLUXSQADUDCSB-UHFFFAOYSA-N phthalaldehyde Chemical compound O=CC1=CC=CC=C1C=O ZWLUXSQADUDCSB-UHFFFAOYSA-N 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 1
- 150000005837 radical ions Chemical class 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000004885 tandem mass spectrometry Methods 0.000 description 1
- 238000010846 tandem mass spectrometry analysis Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The application provides an amino acid synthesis device and a method, wherein the method comprises the following steps: heating the water storage device to enable water in the water storage device to generate steam and enter the discharge reaction cavity; respectively introducing nitrogen and carbon dioxide through an air source inlet to form mixed gas and introducing the mixed gas into the discharge reaction cavity; the electric needle is electrified through a high-voltage power supply so as to ionize water vapor in the discharge reaction cavity to generate water radical cation clusters, and water radical cations in the water radical cation clusters react with the mixed gas to synthesize amino acid. The amino acid is synthesized by the amino acid synthesis method, no additional chemical catalyst is needed, energy is saved, and the method is green and pollution-free.
Description
Technical Field
The application relates to the technical field of amino acid synthesis, in particular to amino acid synthesis equipment and method.
Background
Water is involved in almost all chemical processes of life, and plays an important role in the fields of life process, daily life, social production and the like. Research on water has been a focus of attention of scientists. The development of gas-phase water radical cation chemical research has important scientific value for explaining some basic scientific problems in the fields of life origin, life aging, energy conversion, chemical synthesis and the like. For example, water radical cations play an important role in natural processes including proton transfer, hydrogen bond formation, cell damage, and atmospheric and interstellar chemistry. Thus, studies related to water radical cations have shown a growing trend worldwide. In the field of application research, the potential value of water radical cations in the field of radiobiology and clinical medicine is of great concern. The water radical cation can also be used as a primary ion in mass spectrometry, so that the sensitivity and selectivity of mass spectrometry detection are improved efficiently. In addition, water radical cations having high reactivity have catalytic activity for chemical reactions.
Amino acids are organic compounds containing basic amino groups and acidic carboxyl groups. A compound formed by substituting the hydrogen atom on the carbon atom of carboxylic acid with amino.
In the prior art, in the process of synthesizing amino acid, a chemical catalyst is generally required to be additionally added, which is not beneficial to green environmental protection and improves the synthesis cost of amino acid.
Disclosure of Invention
Based on the above, the application aims to provide an amino acid synthesis device and an amino acid synthesis method, which are used for solving the technical problems that in the prior art, a chemical catalyst is additionally added in the preparation of amino acid, and the amino acid synthesis cost is improved while the environment is not protected.
The application provides amino acid synthesis equipment, which comprises a water storage device, a discharge reaction cavity and a condensing device, wherein the water storage device, the discharge reaction cavity and the condensing device are sequentially connected end to end, the water storage device is used for storing water and heating to generate water vapor, and the discharge reaction cavity is used for providing a reaction space for gas reaction;
the amino acid synthesis equipment is also provided with a sample collection area and at least two air source inlets, wherein the sample collection area is arranged between the condensing device and the water storage device, the two air source inlets are arranged at intervals and between the water storage device and the discharge reaction cavity, and the sample collection area is provided with a collection port used for collecting amino acids.
In addition, the amino acid synthesis apparatus according to the present application may further have the following additional technical features:
further, a gas inlet and two electric needle openings are arranged above the discharge reaction cavity, the two electric needle openings are respectively arranged at two sides of the gas inlet, the two electric needle openings are oppositely arranged, and water vapor generated by water in the water storage device enters the discharge reaction cavity through the gas inlet to react so as to synthesize amino acid;
the amino acid synthesis equipment further comprises two electric needles, the needle tip ends of the electric needles penetrate through the electric needle openings and are arranged in the discharge reaction cavity, the connection ends of the two electric needles are respectively connected with the positive electrode and the negative electrode of the high-voltage power supply, and the needle tip ends of the electric needles are arranged at intervals.
Further, the distance between the tip ends of the two electric needles is 3 mm-8 mm.
Further, the radius of curvature of the needle tip of the electric needle is 0.01 mm-0.1 mm.
Further, an amino acid detection color reagent or water or other absorbent solution for enriching amino acid is arranged in the discharge reaction cavity, and the amino acid detection color reagent is ninhydrin.
In another aspect, the present application provides an amino acid synthesis method, using the above amino acid synthesis apparatus, the amino acid synthesis method comprising:
heating the water storage device to enable water in the water storage device to generate steam and enter the discharge reaction cavity;
respectively introducing nitrogen and carbon dioxide through an air source inlet to form mixed gas and introducing the mixed gas into the discharge reaction cavity;
the electric needle is electrified through a high-voltage power supply so as to ionize water vapor in the discharge reaction cavity to generate water radical cation clusters, water radical cations in the water radical cation clusters and nitrogen in the mixed gas generate ammonia substances, the water radical cations in the water radical cation clusters and carbon dioxide in the mixed gas generate carboxylic acid substances, and the generated ammonia substances react with the carboxylic acid substances to synthesize amino acids, wherein the amino acids comprise glycine, alanine and aspartic acid.
In addition, the above-mentioned amino acid synthesis method according to the present application may further have the following additional technical features:
further, in the step of introducing nitrogen and carbon dioxide through the gas source inlets to form a mixed gas and introducing the mixed gas into the discharge reaction chamber, respectively:
the flow rate of the nitrogen is 0.1-L/min to 0.4L/min.
Further, in the step of introducing nitrogen and carbon dioxide through the gas source inlets to form a mixed gas and introducing the mixed gas into the discharge reaction chamber, respectively:
the flow rate of the carbon dioxide is 0.1-L/min to 0.4L/min.
Further, in the step of energizing the electric needle by the high voltage power supply to ionize the water vapor in the discharge reaction chamber to generate water radical cation clusters:
the discharge voltage in the discharge reaction cavity is 5kV-9 kV.
Further, in the step of energizing the electric needle by the high voltage power supply to ionize the water vapor in the discharge reaction chamber to generate water radical cation clusters:
the reaction time in the discharge reaction cavity is 24-72 h.
Firstly, heating a water storage device to enable water vapor in the water storage device to enter a discharge reaction cavity; secondly, respectively introducing nitrogen and carbon dioxide through an air source inlet to form mixed gas and introducing the mixed gas into a discharge reaction cavity; in addition, the electric needle is electrified through a high-voltage power supply so as to ionize water vapor in the discharge reaction cavity to generate a water radical cation cluster, water radical cations in the water radical cation cluster and nitrogen in the mixed gas generate ammonia substances, the water radical cations in the water radical cation cluster and carbon dioxide in the mixed gas generate carboxylic acid substances, and the generated ammonia substances react with the carboxylic acid substances to synthesize amino acid; the amino acid synthesis method provided by the application can be used for synthesizing amino acid without adding any chemical catalyst, so that energy is saved, and the method is green and pollution-free.
Drawings
FIG. 1 is a schematic diagram of an amino acid synthesis apparatus according to an embodiment of the present application;
FIG. 2 is a step diagram of an amino acid synthesis method according to an embodiment of the present application;
FIG. 3 is a graph of a control mass spectrum of amino acids synthesized by the action of water radical cations with nitrogen and carbon dioxide and standard amino acids;
FIG. 4 is a control liquid chromatogram of amino acid synthesized by the action of water radical cations with nitrogen and carbon dioxide and standard amino acid;
main device symbol description:
the application will be further described in the following detailed description in conjunction with the above-described figures.
Detailed Description
In order that the application may be readily understood, a more complete description of the application will be rendered by reference to the appended drawings. Several embodiments of the application are presented in the figures. This application may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "mounted" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
In order to solve the technical problems that in the prior art, an additional chemical catalyst is needed to be added for preparing the amino acid, and the synthesis cost of the amino acid is increased while the environment is not protected, the application provides the amino acid synthesis equipment and the amino acid synthesis method, which are free from the environmental protection, and the energy is saved without the additional chemical catalyst.
Specifically, as shown in fig. 1, in one aspect, the present application provides an amino acid synthesis apparatus, which includes a water storage device 600, a discharge reaction cavity 300 and a condensation device 500 connected end to end in sequence, wherein the water storage device 600 is used for storing water and heating to generate steam, and the discharge reaction cavity 300 is used for providing a reaction space for gas reaction;
the amino acid synthesis equipment is also provided with a sample collection area 900 and at least two air source inlets, wherein the sample collection area 900 is arranged between the condensing device 500 and the water storage device 600, the two air source inlets are arranged at intervals and are arranged between the water storage device 600 and the discharge reaction cavity 300, and the sample collection area 900 is provided with a collection port used for collecting amino acids.
In order to construct a closed environment, the reactant is conveniently circulated in a closed state, and as a specific example, the water storage device 600, the discharge reaction chamber 300 and the condensing device are connected end to end, thereby improving the reaction efficiency. Further, by providing condensing means, more amino acid product is collected.
The water storage device 600 is communicated with the discharge reaction chamber 300 through the reactant input channel 400, the gas source inlet comprises a first pipeline 100 and a second pipeline 200, the first pipeline 100 and the second pipeline 200 are both arranged on the reactant input channel 400, as a specific example, the first pipeline 100 and the second pipeline 200 are respectively communicated with a nitrogen gas source and a carbon dioxide gas source, and nitrogen gas and carbon dioxide enter the discharge reaction chamber 300 through the reactant input channel 400.
As a specific example, the discharge reaction chamber 300 is a three-hole flask structure, specifically, a gas inlet and two electric needle openings respectively arranged at two sides of the gas inlet are arranged above the discharge reaction chamber 300, the two electric needle openings are oppositely arranged, water vapor generated by water in the water storage device 600 enters the discharge reaction chamber 300 through the gas inlet to react so as to synthesize amino acid, the water vapor enters the discharge reaction chamber 300 through the reactant input channel 400 and the gas inlet, the reactant input channel 400 is communicated to the water storage device 600, and the water vapor can be conveniently introduced into the discharge reaction chamber 300 through heating and kept at a certain humidity. The bottom of the discharge reaction chamber 300 is connected with a condensing device 500, and the output end of the condensing device 500 is connected with a water storage device 600 through a product output channel 800.
In this embodiment, the amino acid synthesis apparatus further includes two electric pins 700, the tip ends of the electric pins 700 are disposed in the discharge reaction cavity 300 through the electric pin openings, the connection ends of the two electric pins 700 are respectively connected to the positive electrode and the negative electrode of the high-voltage power supply, and the tip ends of the electric pins 700 are disposed at a distance. As a specific example, the tip ends of the two electric needles 700 are spaced apart by 3mm to 8mm, and the radius of curvature of the needle tips of the electric needles 700 is 0.01mm to 0.1mm. It should be further noted that, the discharge reaction chamber 300 is provided with an amino acid detection color-developing agent or water or other absorbent solution for enriching amino acids, and the amino acid detection color-developing agent is ninhydrin.
The condensing device 500 is connected to a water inlet and a water outlet for condensing the formed amino acid product and passing through the product output channel 800 to the sample collecting area 900, after the reaction is finished, the formed product is condensed to the sample collecting area 900, and an auxiliary agent may be added to the sample collecting area 900 to confirm whether amino acid is generated or not, or to facilitate the removal of the product, as a specific example, the auxiliary agent may be water or 8-hydroxyquinoline solution.
In another aspect, the present application provides an amino acid synthesis method implemented by the above-mentioned amino acid synthesis apparatus, please refer to fig. 2, the amino acid synthesis method includes steps S101-S103:
s101, heating the water storage device to enable water in the water storage device to generate steam and enable the steam to enter the discharge reaction cavity.
S102, respectively introducing nitrogen and carbon dioxide through an air source inlet to form mixed gas and introducing the mixed gas into the discharge reaction cavity.
In some alternative embodiments, the discharge voltage in the discharge reaction chamber is 5kV-9kV, and in some alternative embodiments, the discharge voltage of the discharge electrode needle is preferably 8kV. The reaction time in the discharge reaction cavity is 24-72 h.
S103, electrifying the electric needle through a high-voltage power supply to ionize water vapor in the discharge reaction cavity to generate a water radical cation cluster, wherein water radical cations in the water radical cation cluster and nitrogen in the mixed gas generate ammonia substances, and the water radical cations in the water radical cation cluster and carbon dioxide in the mixed gas generate carboxylic acid substances, so that the generated ammonia substances react with the carboxylic acid substances to synthesize amino acid.
Amino acids include glycine, alanine and aspartic acid. In some alternative embodiments, the flow rates of nitrogen and carbon dioxide are each 0.1L/min to 0.4L/min.
Specifically, firstly, water is heated to enable liquid water in the water storage device to form water vapor, the water vapor enters the discharge reaction cavity through the reactant input channel, and mixed gas of nitrogen and carbon dioxide enters the reactant input channel through the first pipeline and the second pipeline and finally enters the discharge reaction cavity;
and secondly, starting a high-voltage power supply, applying high voltage to the electric needle, enabling the needle tip of the discharge array needle to generate spark discharge, and ionizing surrounding water vapor to form water radical cation clusters. Specifically, a high voltage of 5kV-9kV is applied to the discharge needle, spark discharge is generated at the tip of the discharge needle, nitrogen is used as a nitrogen source, carbon dioxide is used as a carbon source at normal temperature and normal pressure (for example, 25 ℃ and one atmosphere pressure), and water radical ion clusters are generated through the high voltage spark discharge. The formed water free radical cation clusters react with nitrogen and carbon dioxide to generate amino acid molecules, and the amino acid molecules are formed into a liquid state through a condensing device and are further transferred into a sample collecting area through a product output channel to form an amino acid aqueous solution;
finally, the aqueous amino acid solution formed in the sample collection area is removed and collected by opening the valve switch.
In order to facilitate an understanding of the application, several embodiments of the application will be presented below. This application may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Example 1
The amino acid synthesis method in the first embodiment of the present application includes:
introducing nitrogen (with a control flow of 0.1L/min) and carbon dioxide (with a control flow of 0.1L/min) into an input channel of a reaction device at normal temperature and normal pressure, applying high voltage (8 kV) to an electric needle along with water vapor molecules formed by heating reaching a discharge reaction cavity, reacting for 24 hours, condensing the generated amino acid molecules, and outputting the condensed amino acid molecules to a sample collecting area through a product output channel to obtain the amino acid.
Example two
The amino acid synthesis method in the second embodiment of the present application includes:
introducing nitrogen (with a control flow of 0.1L/min) and carbon dioxide (with a control flow of 0.1L/min) into an input channel of a reaction device at normal temperature and normal pressure, applying high voltage (8 kV) to an electric needle along with water vapor molecules formed by heating reaching a discharge reaction cavity, reacting for 48 hours, condensing the generated amino acid molecules, and outputting the condensed amino acid molecules to a sample collecting area through a product output channel to obtain the amino acid.
Example III
The amino acid synthesis method in the third embodiment of the present application includes:
introducing nitrogen (with a control flow of 0.1L/min) and carbon dioxide (with a control flow of 0.1L/min) into an input channel of a reaction device at normal temperature and normal pressure, applying high voltage (8 kV) to an electric needle along with water vapor molecules formed by heating reaching a discharge reaction cavity, reacting for 72 hours, condensing the generated amino acid molecules, and outputting the condensed amino acid molecules to a sample collecting area through a product output channel to obtain the amino acid.
The synthesis results of the amino acid synthesis method of the present application were verified as follows:
test one:
taking the synthesis result of the amino acid synthesis method of the third embodiment as an example, taking the product of the third embodiment for mass spectrometry and chromatographic detection analysis respectively. From the mass spectrum data results, significantly enhanced ion peaks of glycine, alanine, aspartic acid, etc., m/z 76, m/z 90 and m/z 134, respectively, can be observed, as specifically shown as a in fig. 3. These newly generated amino acid product ions m/z 76, m/z 90 and m/z 134 were further screened for tandem mass spectrometry analysis, and the data results are shown as b in FIG. 3 through d in FIG. 3, respectively. Tandem mass spectrometry data for these product ions all showed typical loss of H 2 O and (CO+H) 2 O) characteristic ions, which are typical missing fragment ion characteristics of amino acids. Meanwhile, the secondary tandem mass spectrogram of the product ions is consistent with the tandem mass spectrum data result of the corresponding standard substanceE in fig. 3 is a tandem mass spectrum of glycine standard, f in fig. 3 is a tandem mass spectrum of alanine standard, and g in fig. 3 is a tandem mass spectrum of aspartic acid standard. Indicating the formation of glycine, alanine and aspartic acid in the reaction solution.
And II, testing:
the sample of amino acids was derivatized using the method of phthalaldehyde. After the derivatization optimization method, glycine, alanine and aspartic acid were detected using a 20mM ammonium acetate aqueous solution (pH 6.5) -acetonitrile (85:15, V/V) gradient elution method. FIG. 4 is a liquid chromatogram of the reaction collection solution and the standard derived from glycine, alanine and aspartic acid, specifically, a in FIG. 4 is a liquid chromatogram of the standard mixed solution; b in fig. 4 is a liquid chromatogram of the reactant sample solution. As can be seen from the data in FIG. 4, glycine, alanine and aspartic acid were all detected in the reaction harvest at the corresponding retention times. This is consistent with experimental data of mass spectrometry.
In summary, the amino acid synthesis method in the above embodiment of the present application can be performed only at normal temperature and normal pressure (referred to as pressure), and the whole synthesis process is environment-friendly and pollution-free, without using a chemical catalyst, and energy saving.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing examples illustrate only a few embodiments of the application and are described in detail herein without thereby limiting the scope of the application. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application. Accordingly, the scope of protection of the present application is to be determined by the appended claims.
Claims (7)
1. The amino acid synthesis equipment is characterized by comprising a water storage device, a discharge reaction cavity and a condensing device which are connected end to end in sequence, wherein the water storage device is used for storing water and heating to generate water vapor, and the discharge reaction cavity is used for providing a reaction space for gas reaction;
the amino acid synthesis equipment is also provided with a sample collection area and at least two air source inlets, wherein the sample collection area is arranged between the condensing device and the water storage device, the two air source inlets are arranged at intervals and are arranged between the water storage device and the discharge reaction cavity, and the sample collection area is provided with a collection port used for collecting amino acids;
a gas inlet and two electric needle openings which are respectively arranged at two sides of the gas inlet are arranged above the discharge reaction cavity, the two electric needle openings are oppositely arranged, and water vapor generated by water in the water storage device enters the discharge reaction cavity through the gas inlet to react so as to synthesize amino acid;
the amino acid synthesis equipment further comprises two electric needles, the distance between the needle tips of the two electric needles is 3 mm-8 mm, the radius of curvature of the needle tips of the electric needles is 0.01 mm-0.1 mm, the needle tips of the electric needles penetrate through the electric needle openings and are arranged in the discharge reaction cavity, the connection ends of the two electric needles are respectively connected with the positive electrode and the negative electrode of the high-voltage power supply, and the distance between the needle tips of the electric needles is set.
2. The amino acid synthesis apparatus according to claim 1, wherein an amino acid detection color reagent or water or other absorbent solution for enriching amino acids is provided in the discharge reaction chamber, and the amino acid detection color reagent is ninhydrin.
3. An amino acid synthesis method, characterized in that it is achieved by the amino acid synthesis apparatus according to any one of the preceding claims 1 to 2, comprising:
heating the water storage device to enable water in the water storage device to generate steam and enter the discharge reaction cavity;
respectively introducing nitrogen and carbon dioxide through an air source inlet to form mixed gas and introducing the mixed gas into the discharge reaction cavity;
the electric needle is electrified through a high-voltage power supply so as to ionize water vapor in the discharge reaction cavity to generate water radical cation clusters, water radical cations in the water radical cation clusters and nitrogen in the mixed gas generate ammonia substances, the water radical cations in the water radical cation clusters and carbon dioxide in the mixed gas generate carboxylic acid substances, and the generated ammonia substances react with the carboxylic acid substances to synthesize amino acids, wherein the amino acids comprise glycine, alanine and aspartic acid.
4. The method according to claim 3, wherein in the step of forming a mixed gas by introducing nitrogen and carbon dioxide through the gas source inlets, respectively, and introducing the mixed gas into the discharge reaction chamber:
the flow rate of the nitrogen is 0.1-L/min to 0.4L/min.
5. The method according to claim 3, wherein in the step of forming a mixed gas by introducing nitrogen and carbon dioxide through the gas source inlets, respectively, and introducing the mixed gas into the discharge reaction chamber:
the flow rate of the carbon dioxide is 0.1-L/min to 0.4L/min.
6. The method according to claim 3, wherein in the step of energizing the electric needle by a high voltage power supply to ionize the water vapor in the discharge reaction chamber to generate water radical cation clusters:
the discharge voltage in the discharge reaction cavity is 5kV-9 kV.
7. The method according to claim 3, wherein in the step of energizing the electric needle by a high voltage power supply to ionize the water vapor in the discharge reaction chamber to generate water radical cation clusters:
the reaction time in the discharge reaction cavity is 24-72 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311499489.7A CN117225336A (en) | 2023-11-13 | 2023-11-13 | Amino acid synthesis equipment and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311499489.7A CN117225336A (en) | 2023-11-13 | 2023-11-13 | Amino acid synthesis equipment and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117225336A true CN117225336A (en) | 2023-12-15 |
Family
ID=89098674
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311499489.7A Pending CN117225336A (en) | 2023-11-13 | 2023-11-13 | Amino acid synthesis equipment and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117225336A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0267254A (en) * | 1988-09-02 | 1990-03-07 | Tokyo Gas Co Ltd | Production of 1-2c nitrogen-containing compound |
| JPH05229996A (en) * | 1992-02-20 | 1993-09-07 | Norihiko Fujita | Production of glycine |
| JP2005281300A (en) * | 2004-03-05 | 2005-10-13 | Japan Science & Technology Agency | Method for producing amino acid using graphite as raw material |
| JP2006306728A (en) * | 2005-04-26 | 2006-11-09 | National Institute Of Advanced Industrial & Technology | Method for producing amino acid |
| EP4000657A1 (en) * | 2020-11-18 | 2022-05-25 | B. Braun Surgical, S.A. | Process for producing amino acids and a use thereof |
| CN116926619A (en) * | 2022-04-11 | 2023-10-24 | 中山大学 | Amino acid synthesis method |
-
2023
- 2023-11-13 CN CN202311499489.7A patent/CN117225336A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0267254A (en) * | 1988-09-02 | 1990-03-07 | Tokyo Gas Co Ltd | Production of 1-2c nitrogen-containing compound |
| JPH05229996A (en) * | 1992-02-20 | 1993-09-07 | Norihiko Fujita | Production of glycine |
| JP2005281300A (en) * | 2004-03-05 | 2005-10-13 | Japan Science & Technology Agency | Method for producing amino acid using graphite as raw material |
| JP2006306728A (en) * | 2005-04-26 | 2006-11-09 | National Institute Of Advanced Industrial & Technology | Method for producing amino acid |
| EP4000657A1 (en) * | 2020-11-18 | 2022-05-25 | B. Braun Surgical, S.A. | Process for producing amino acids and a use thereof |
| CN116926619A (en) * | 2022-04-11 | 2023-10-24 | 中山大学 | Amino acid synthesis method |
Non-Patent Citations (6)
| Title |
|---|
| BYSTANLEY L, ET AL.: "ProductionofSome OrganicCompounds underPossiblePrimitiveEarthConditions", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 77, pages 2352 - 2361 * |
| F. HANIC ET AL.: "THERMOCHEMICAL ASPECTS OF THE CONVERSION OF THE GASEOUS SYSTEM CO2-N 2-H 2O INTO A SOLID MIXTURE OF AMINO ACIDS", JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, vol. 60, pages 1113 - 1120 * |
| M. JANDA, ET AL.: "Study of Plasma Induced Chemistry by DC Discharges in CO 2 /N 2 /H 2 O Mixtures Above a Water Surface", ORIG LIFE EVOL BIOSPH * |
| Y. HIROSE ET AL.: "SYNTHESIS OF BIOMOLECULES FROM N2, CO, AND H20 BY ELECTRIC DISCHARGE", ORIGINS OF LIFE AND EVOLUTION OF THE BIOSPHERE * |
| 李建军: "你家后院有恐龙吗 恐龙世界探密记", 31 May 2021, 上海科学技术文献出版社, pages: 13 * |
| 王金亭: "生命科学导论", 30 September 2014, 华中科技大学出版社, pages: 281 - 282 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Heiles | Advanced tandem mass spectrometry in metabolomics and lipidomics—methods and applications | |
| Banerjee et al. | Electrospray ionization mass spectrometry: a technique to access the information beyond the molecular weight of the analyte | |
| Buchanan et al. | Fourier transform mass spectrometry of high-mass biomolecules | |
| CN109200970A (en) | Low-temperature plasma dual field assists device and the application of gas phase reaction synthesis compound | |
| Xu et al. | Immobilization of trypsin on graphene oxide for microwave-assisted on-plate proteolysis combined with MALDI-MS analysis | |
| Yan et al. | Recent advances in proteomics and metabolomics in plants | |
| CN108990248A (en) | A kind of plasma producing apparatus and its application | |
| US20070284523A1 (en) | Process and apparatus for 15-nitrogen isotope determination of condensed phase samples | |
| Pope et al. | Structure of the host-specific toxin produced by Helminthosporium carbonum | |
| Xiao et al. | Top-down protein identification using isotopic envelope fingerprinting | |
| Sidoli et al. | SWATH analysis for characterization and quantification of histone post-translational modifications | |
| CN117225336A (en) | Amino acid synthesis equipment and method | |
| March et al. | Practical Aspects of Trapped Ion Mass Spectrometry, Volume V: Applications of Ion Trapping Devices | |
| Zhu et al. | Arc plasma-based dissociation device: fingerprinting mass spectrometric analysis realized at atmospheric condition | |
| Lee et al. | Fourier transform ion cyclotron resonance study of multiply charged aggregates of small singly charged peptides formed by electrospray ionization | |
| KR100522964B1 (en) | Method and apparatus for generating hydrogen gas by direct thermal decomposition of water | |
| Kubwabo et al. | Characterization of microcystins using in‐source collision‐induced dissociation | |
| CN100454020C (en) | Gas-phase molecular ion reactor based on radio-frequency quard-polar rod, its realization and use | |
| CN106198790A (en) | A kind of horse and mule common characteristic polypeptide and application thereof | |
| CN209250931U (en) | A kind of plasma producing apparatus | |
| Magnera et al. | Triple-quadrupole secondary ion mass spectrometry of low-temperature solids: collision-activated dissociation of large cluster ions | |
| CN110873766A (en) | Mass spectrometry method for screening protein with structure and interaction change caused by drug | |
| CN108786393A (en) | A kind of room temperature decomposition method of carbon dioxide | |
| US11567084B2 (en) | Microwave enhanced enzymatic reactor for proteomics by mass spectrometry | |
| CN212932486U (en) | Device for distinguishing hexenol isomers by taking water as derivative reagent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |