CN117191472A - Sample and liquid storage integrated detection reagent card - Google Patents

Abstract

The invention discloses a detection reagent card integrating sampling and liquid storage, which comprises a side flow chromatography device and a sampling device which are connected in a plug-in manner; the sampling device comprises a sampling section and a liquid storage section, wherein the sampling section is provided with a sampling part and a hollow flow channel, the liquid storage section is provided with a liquid storage cavity for storing fluid, the sampling section and the liquid storage section are provided with sealing structures, the sealing structures separate the hollow flow channel and the opening, and the fluid is sealed in the liquid storage cavity. Along with the relative motion of the sampling section and the liquid storage section, the sealing structure is opened or destroyed, and the hollow flow channel is communicated with the opening, so that the fluid in the liquid storage cavity can flow onto the sampling component, and the operation is simple and quick. The arrangement of the sampling section and the liquid storage section can realize the integrated carrying and storage of the fluid and the sampling component, can be used for fluid sampling, and can be used for diluting the acquired fluid sample so as to quickly finish sampling and diluting; the device is also suitable for solid sampling, and the fluid is used for processing the sampling component before sampling, so that the solid sampling effect is improved.

Description

Sample and liquid storage integrated detection reagent card

Technical Field

The invention relates to the technical field of sample sampling, in particular to a detection reagent card integrating sampling and liquid storage.

Background

Sample sampling techniques are widely used in a variety of fields such as food detection, drug detection, environmental monitoring, clinical diagnostics, and the like. The sample sampling technique is a technique for acquiring a sample to be detected before the detection is implemented, and is different from the direct detection. For example, in vitro diagnosis and environmental sampling detection, in vitro diagnosis refers to a technique of detecting a human body sample (blood, body fluid, tissue, etc.) outside a human body to obtain clinical diagnosis information and further judging a disease or a body function. The in vitro diagnostic product mainly consists of diagnostic equipment (instruments) and diagnostic reagents. According to the standard of the medical apparatus and instrument classification catalogue of the national food and drug administration (CFDA), the in vitro diagnosis equipment belongs to the clinical examination analysis instrument class. Environmental sampling detection refers to a method for acquiring detection information by sampling and detecting environmental samples such as water, soil, gas and object surfaces.

In the technical field of in-vitro diagnosis, the sampling modes of samples are various, wherein the sampling modes of saliva and sweat sampling have the problems of small sampling amount and large sample viscosity, and the conditions of low sample feeding speed and even no sample feeding of a later chromatographic test strip are easy to cause, so that the acquired samples are required to be diluted; in the sampling modes of powder wiping sampling, environment wiping sampling and the like, a pretreatment solution is adopted to pretreat a sampling component to achieve a good sampling effect, the existing sampling component and a diluent or pretreatment solution are usually packaged separately and used in a plurality of operations, specifically, the sampling component is individually sterilized and packaged in a plastic package, and meanwhile, bottled diluent or pretreatment solution is additionally provided for multi-step operation. Therefore, there is a need to provide a solution for reducing the sampling procedure and helping to reduce the sampling operations of the sampling personnel.

Disclosure of Invention

The present invention aims to at least solve the technical problems existing in the prior art. Therefore, the invention provides a detection reagent card which is provided with a sampling component and a liquid storage component and is convenient for adding fluid to the sampling component and integrating sampling and liquid storage.

The first aspect of the present invention provides a sample and liquid storage integrated detection reagent card, comprising:

the lateral flow chromatography device is connected with the sampling device in a plug-in mode;

the sampling device is used for sampling and storing fluid, and comprises:

the sampling section is provided with a sampling component, and a hollow runner connected with the sampling component is arranged in the sampling section;

the liquid storage section is provided with a liquid storage cavity for storing the fluid, and the liquid storage cavity is provided with an opening for the fluid to flow out;

the liquid storage device comprises a liquid storage section, a sampling section and a liquid storage section, wherein a sealing structure used for blocking the hollow flow channel from being communicated with the opening is arranged between the sampling section and the liquid storage section, and the sampling section and the liquid storage section can move relatively so as to open or destroy the sealing structure.

According to some embodiments of the invention, the liquid storage section is further provided with a containing cavity, the sealing structure is arranged between the containing cavity and the liquid storage cavity, and the sampling section is movably installed in the containing cavity.

According to some embodiments of the invention, the sampling section is connected with the liquid storage section through a connecting piece, the connecting piece comprises a fixing part and a connecting section, the fixing part is installed on the liquid storage section, the sampling section is movably installed in the connecting section, the liquid storage cavity is connected with the sampling section through the connecting section, and the sealing structure seals an opening communicated with the liquid storage cavity on the connecting section.

According to some embodiments of the invention, the sampling section is provided with a first clamping groove and a second clamping groove, the liquid storage section is provided with a buckle, and the sampling section and the liquid storage section are movably connected through the matching of the clamping grooves and the buckles;

when the buckle is positioned in the first clamping groove, the sealing structure cuts off the hollow runner from the opening;

when the buckle is positioned in the second clamping groove, the sealing structure is opened or destroyed, and the hollow runner is communicated with the opening.

According to some embodiments of the invention, the sampling section is further provided with a separable protection piece, the liquid storage section is provided with a limiting part matched with the protection piece, and the protection piece abuts against the limiting part to limit the movement of the buckle from the first clamping groove to the second clamping groove.

According to some embodiments of the invention, the liquid storage section is provided with a liquid outlet pipe, the opening is arranged on the side wall of the liquid outlet pipe, the sealing structure is movably sleeved on the liquid outlet pipe and seals the opening, and the sampling section is provided with an abutting part corresponding to the sealing structure.

According to some embodiments of the invention, the reservoir is further provided with a self-sealing member comprising a protective cap arranged on a side of the reservoir remote from the sampling section, a piston connected to the protective cap by the push member, and a push member arranged between the piston and the protective cap.

According to some embodiments of the invention, the sampling section is provided with a limiting protrusion, and the liquid storage section is provided with a limiting groove matched with the limiting protrusion; or, the liquid storage section is provided with a limiting protrusion, and the sampling section is provided with a limiting groove matched with the limiting protrusion.

According to some embodiments of the invention, a volume indicator is also disposed between the sampling member and the sampling segment.

According to some embodiments of the invention, the lateral flow chromatography device comprises a shell and a detection test strip, wherein the detection test strip is positioned in the shell, the shell is provided with a sample inlet, the sampling device is inserted into the sample inlet through the sampling component and is connected with the lateral flow chromatography device, a sample channel is arranged between the sample inlet and the detection test strip, and the sample flows to the detection test strip through the sample channel.

The sample and liquid storage integrated detection reagent card provided by the invention has at least the following beneficial effects: the invention relates to a detection reagent card integrating sampling and liquid storage, which comprises a lateral flow chromatography device and a sampling device which are connected in a plug-in manner; wherein sampling device includes the sampling section and deposits the liquid section, and the sampling section is provided with sampling part and cavity runner, and the liquid section of depositing is provided with the liquid chamber of depositing that is used for storing fluid, and the sampling section sets up seal structure with the liquid section of depositing, and seal structure cuts off cavity runner and opening, can hinder cavity runner and opening intercommunication, with fluid seal deposit the intracavity. Through the relative motion of sampling section and deposit the liquid section, can open or destroy seal structure, make the opening that deposits the liquid chamber and the cavity runner intercommunication of sampling section, make the fluid flow that deposits the liquid intracavity to the sample part on, easy operation is swift. The setting that sampling section links to each other with the liquid section that holds can realize fluid and sampling part's an organic whole and carry and store, both are applicable to the fluid sample: diluting the obtained fluid sample, and rapidly completing sampling and dilution; and is also suitable for solid sampling: the fluid is used for treating the sampling component before sampling, so that the solid sampling effect is improved, the universality is good, and the application range is wide. The sampling and liquid storage integrated detection reagent card provided by the invention is applied to the fields of in-vitro diagnosis and environmental sampling detection, and can greatly improve the detection efficiency.

Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.

Drawings

The foregoing and/or additional aspects and advantages of the invention will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:

FIG. 1 is a schematic diagram of a sampling device according to a first embodiment of the present invention;

FIG. 2 is a cross-sectional view of a sealing structure separating a hollow flow passage from an opening in accordance with a first embodiment of the present invention;

FIG. 3 is a cross-sectional view showing a broken seal structure in accordance with a first embodiment of the present invention;

FIG. 4 is a schematic diagram of a sampling device according to a second embodiment of the present invention;

FIG. 5 is a cross-sectional view of a sealing structure separating a hollow flow passage from an opening in a second embodiment of the present invention;

FIG. 6 is a cross-sectional view showing a broken seal structure in a second embodiment of the present invention;

FIG. 7 is a schematic diagram of a sampling device according to a third embodiment of the present invention;

FIG. 8 is a cross-sectional view showing a broken seal structure in a third embodiment of the present invention;

FIG. 9 is a schematic diagram of a sampling device according to a fourth embodiment of the present invention;

FIG. 10 is a cross-sectional view of a sealing structure separating a hollow flow passage from an opening in a fourth embodiment of the present invention;

FIG. 11 is a cross-sectional view showing a broken seal structure in a fourth embodiment of the present invention;

FIG. 12 is a schematic view of a lateral flow chromatography device in an embodiment of the invention;

FIG. 13 is an exploded view of a sample and reservoir integrated test reagent card and lateral flow chromatography device in accordance with an embodiment of the present invention.

Reference numerals:

the sampling section 100, the hollow pipeline 110, the limiting protrusion 120, the protecting piece 130, the buckle 140 and the abutting part 150;

the liquid storage section 200, the liquid storage cavity 210, the accommodating cavity 220, the limiting groove 221, the first clamping groove 231, the second clamping groove 232, the liquid outlet pipe 240 and the opening 241;

a sampling part 300;

the self-sealing member 400, the protective cap 410, the push rod 420, the piston 430, the spring 440;

a sealing structure 500;

a connector 600, a fixing portion 610, a connecting section 620;

lateral flow chromatography detection apparatus 700, upper housing 710, observation port 711, lower housing 720, sample inlet 721, sample channel 722, and test strip 730.

Detailed Description

Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the invention.

In the description of the present invention, it should be understood that references to orientation descriptions such as upper, lower, front, rear, left, right, inner, outer, etc. are based on the orientation or positional relationship shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.

In the description of the present invention, a number means one or more, a number means two or more, and greater than, less than, exceeding, etc. are understood to not include the present number, and above, below, within, etc. are understood to include the present number. The description of the first and second is for the purpose of distinguishing between technical features only and should not be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless explicitly defined otherwise, terms such as arrangement, mounting, connection, assembly, cooperation, etc. should be construed broadly and the specific meaning of the terms in the present invention can be reasonably determined by a person skilled in the art in combination with the specific contents of the technical solution.

The following describes a sampling device in a sample-reservoir integrated detection reagent card according to an embodiment of the present application with reference to fig. 1 to 13.

Referring to fig. 1 to 13, fig. 1 is a schematic structural diagram of a sampling device according to a first embodiment of the present application; FIG. 2 is a cross-sectional view of a sealing structure separating a hollow flow passage from an opening in accordance with a first embodiment of the present application; FIG. 3 is a cross-sectional view showing a broken seal structure in accordance with a first embodiment of the present application; FIG. 4 is a schematic diagram of a sampling device according to a second embodiment of the present application; FIG. 5 is a cross-sectional view of a sealing structure separating a hollow flow passage from an opening in a second embodiment of the present application; FIG. 6 is a cross-sectional view showing a broken seal structure in a second embodiment of the present application; FIG. 7 is a schematic diagram of a sampling device according to a third embodiment of the present application; FIG. 8 is a cross-sectional view showing a broken seal structure in a third embodiment of the present application; FIG. 9 is a schematic diagram of a sampling device according to a fourth embodiment of the present application; FIG. 10 is a cross-sectional view of a sealing structure separating a hollow flow passage from an opening in a fourth embodiment of the present application; FIG. 11 is a cross-sectional view showing a broken seal structure in a fourth embodiment of the present application; FIG. 12 is a schematic view of a lateral flow chromatography device in an embodiment of the application;

FIG. 13 is an exploded view of a sample and reservoir integrated test reagent card and lateral flow chromatography device in accordance with an embodiment of the present application.

Referring to fig. 1 to 11, the sampling device includes a sampling section 100 and a liquid storage section 200, the sampling section 100 is provided with a sampling component 300, the sampling component 300 is connected in a notch of the sampling section, which is far away from the end of the liquid storage section, and the sampling section 100 is also provided with a hollow flow channel 110 connected with the sampling component 300, the liquid storage section 200 is provided with a liquid storage cavity 210 for storing fluid, the liquid storage cavity 210 is provided with an opening 241 for the fluid to flow out, the sampling section 100 is connected with the liquid storage section 200, a sealing structure 500 for blocking the hollow flow channel 110 from being communicated with the opening 241 is provided between the sampling section 100 and the liquid storage section 200, and the sampling section 100 and the liquid storage section 200 can relatively move to open or destroy the sealing structure 500. The sampling part 300 is disposed at one end of the sampling section 100, the hollow flow channel 110 is disposed inside the sampling section 100 and connects the sampling part 300 and the liquid storage cavity 210, the sealing structure 500 is disposed between the hollow flow channel 110 and the opening 241 of the liquid storage cavity 210, the hollow flow channel 110 is isolated from the opening 241, and the liquid is sealed in the liquid storage cavity 210, so that sealing and storage of the liquid can be realized. The sampling section 100 and the liquid storage section 200 are combined, can integrally carry the stored fluid and the sampling component 300, and have good portability, wide application environment and strong universality. When the sampling part 300 is used for collecting saliva samples of the oral cavity, part of the storage section does not enter the oral cavity of a tested person, the leakage risk that the storage section is snapped to cause fluid to enter the oral cavity does not exist, and good safety is achieved.

Referring to fig. 12 and 13, the sample and reservoir integrated detection reagent card comprises a sampling device and a lateral flow chromatography device 700, wherein the lateral flow chromatography device 700 is connected with the sampling device in a plug-in manner; the sampling device is used for sampling and storing fluid. The lateral flow chromatography device 700 comprises a shell and a detection test strip 730, wherein the detection test strip 730 is positioned inside the shell, the shell is provided with a sample inlet 721, the sampling device is inserted into the sample inlet 721 through a sampling component and connected with the lateral flow chromatography device, a sample channel 722 is arranged between the sample inlet and the detection test strip 730, and a sample flows to the detection test strip 730 through the sample channel.

It will be appreciated that in some embodiments, the lateral flow assay device 700 is modular, with the housing comprising upper and lower foldable shells 710, 720, and the test strip 730 is located in the space where the upper and lower shells 710, 720 are folded.

In some embodiments, lateral flow assay device 700 further includes a viewing port 711 provided on upper housing 710 for viewing the response of test strip 730. In some embodiments, a light-transmitting film may also be coated over the viewing port 711 to protect the test strip 730 from contamination.

It will be appreciated that in some embodiments, the sealing structure 500 may be a sealing membrane or plug for plugging disposed between the hollow flow passage 110 and the opening 241. In other embodiments, the sealing structure 500 may also be provided as a plastic material that is easily opened or broken, such as a frangible plastic cap or the like provided at the junction at the opening 241. In some embodiments, the seal 500 may also be configured as a connection that may be pushed apart or opened or broken in one direction, such as a pinch plate mounted at the opening 241 by a snap-fit connection, or the like. In other embodiments, the sealing structure 500 may be made of a material that is easily pierced, such as aluminum foil, plastic, or other materials that are easily pierced.

The sampling section 100 and the liquid storage section 200 are arranged to be capable of relatively moving, the sealing structure 500 can be opened or destroyed through the relative movement between the sampling section 100 and the liquid storage section 200, so that the fluid in the liquid storage cavity 210 can flow into the hollow pipeline through the opening 241, the hollow pipeline is connected with the sampling component 300, and the fluid can flow onto the sampling component 300. It can be appreciated that the relative movement between the sampling section 100 and the liquid storage section 200 may be that the liquid storage section 200 is fixed, and the sampling section 100 moves relative to the liquid storage section 200; the sampling section 100 may be fixed, and the liquid storage section 200 moves relative to the sampling section 100, so that the relative movement between the sampling section 100 and the liquid storage section 200 can be realized.

In some embodiments, the sampling segment 100 and the storage segment 200 are connected by a slot-in-slot clasp 140, and the relative movement between the sampling segment 100 and the storage segment 200 is achieved by a clasp 140 that is movable in a plurality of slots. In other embodiments, the sampling segment 100 and the reservoir segment 200 are connected by threads, and the sampling segment 100 and the reservoir segment 200 can also be moved relatively by screwing in and screwing out.

The detection reagent card integrating sampling and liquid storage can be used for fluid sampling and detection, and can be used for diluting an acquired fluid sample to quickly finish sampling and diluting; the device is also suitable for solid sampling detection of powder and the like, and the sampling component 300 is pretreated by using fluid before sampling, so that the solid sampling effect can be improved, the universality is good, and the application range is wide. Specifically, in some embodiments, the integrated detection reagent card for sampling and storing liquid is used for fluid sampling detection, after the sampling of the fluid is completed by using the sampling component 300 on the sampling section 100, the fluid in the liquid storing cavity 210 flows to the sampling component 300 through the relative movement between the sampling section 100 and the liquid storing section 200, so that the viscosity of the diluted sample is reduced, and the smooth dialysis of the sample during detection is ensured. In some embodiments, the detection reagent card with integrated sampling and liquid storage functions is used for powder sampling detection, before the sampling component 300 performs sampling, the sealing structure 500 is opened or destroyed by the relative motion between the sampling section 100 and the liquid storage section 200, the fluid in the liquid storage cavity 210 flows to the sampling component 300, the sampling component 300 is preprocessed, and the sampling effect of solid sampling performed by the preprocessed sampling component 300 is better. The detection reagent card integrating sampling and liquid storage is suitable for various sampling detection modes such as liquid sampling detection, solid sampling detection and the like, can meet the requirements of biological sample sampling and environment wiping sampling, and has good flexibility and universality. Fluid can be added to the sampling member 300 through the relative movement between the sampling section 100 and the reservoir section 200, and the operation is simple and quick, reducing the complexity of the detection operation.

It should be noted that the sample taken by the sampling member 300 of the present application may be any fluid or solid sample, and examples of samples suitable for testing using the present application include saliva, whole blood, serum, plasma, urine, spinal fluid, biological extract, mucus, tissue, powder, and environmental samples. The environment sample refers to any article in the environment outside the human body, and all the articles can be used as the environment sample. Such as: the ground, the sewer pipelines, sewage, table tops, all articles on the table tops for selling fruits, vegetables, meats, chopping boards, cutters, appliances and the like, and the transport vehicle can also be used as an environmental sample.

Example 1

Referring to fig. 1 to 3, a sampling device in a sample-and-reservoir integrated test reagent card according to the present application will be described in detail with reference to a specific embodiment.

In some embodiments, the liquid storage section 200 is further provided with a containing cavity 220, a sealing structure 500 is arranged between the containing cavity 220 and the liquid storage cavity 210, and the sampling section 100 is movably installed in the containing cavity 220. Referring to fig. 2, the liquid storage section 200 includes a liquid storage chamber 210 and a receiving chamber 220, a sealing structure 500 is disposed between the receiving chamber 220 and the liquid storage chamber 210, and the sealing structure 500 isolates the liquid storage chamber 210 from the receiving chamber 220, so that fluid in the liquid storage chamber 210 can be prevented from flowing into the receiving chamber 220. The sampling segment 100 is movably mounted in the accommodating cavity 220, and the sealing structure 500 is disposed between the accommodating cavity 220 and the liquid storage cavity 210, and referring to fig. 3, the sampling segment 100 moves in the accommodating cavity 220 relative to the accommodating cavity 220, so that the sealing structure 500 can be opened or broken, and fluid flows into the hollow flow channel 110 of the sampling segment 100. In some embodiments, the sampling segment 100 is mounted within the receiving cavity 220 by a transition fit, and the sampling segment 100 is movable relative to the receiving cavity 220. It should be noted that, in some other embodiments, the sampling section 100 is sleeved outside the accommodating cavity 220, the hollow flow channel 110 of the sampling section 100 is disposed in the accommodating cavity 220, the accommodating cavity 220 is movably installed in the sampling section 100, and the accommodating cavity 220 and the sampling section 100 are installed through transition fit, so that the relative movement between the sampling section 100 and the liquid storage section 200 can be realized.

It should be noted that, in some embodiments, the liquid storage cavity 210 and the accommodating cavity 220 of the liquid storage section 200 are integrally formed, and the sampling device of the present application is only assembled by two parts of the sampling section 100 and the liquid storage section 200, so that the production and the assembly are convenient, the production and the processing procedures are effectively reduced, and the cost is reduced.

In some embodiments, the hollow conduit is disposed opposite to the sealing structure 500 and the opening 241, and the fluid can directly flow into the hollow conduit after the sealing structure 500 is opened or broken, thereby improving the flow efficiency of the fluid. It will be appreciated that in other embodiments, the hollow flow passage 110 is disposed toward the liquid storage chamber 210, and the hollow flow passage 110 is disposed off-center of the opening 241, as well as capable of receiving and delivering a fluid. In some embodiments, the hollow conduit of the sampling section 100 is provided with a plurality of conduits that communicate the receiving chamber 220 with the sampling member 300, as well as being capable of completing the delivery of fluid to the sampling member 300.

In some embodiments, the reservoir 210 is further provided with a self-sealing member 400, the self-sealing member 400 comprising a protective cap 410, a piston 430 and a pushing member, the protective cap 410 being arranged on a side of the reservoir 210 remote from the sampling section 100, the protective cap 410 sealing off a side of the reservoir 210, the piston 430 being connected to the protective cap 410 by the pushing member, the pushing member being arranged between the piston 430 and the protective cap 410. The self-sealing member 400 seals the fluid storage chamber 210, and in particular, the sealing structure 500 seals the fluid storage chamber 210 at one side of the fluid storage chamber 210, and the piston 430 of the self-sealing member 400 seals the fluid storage chamber 210 at the other side, thereby completing the sealing of both ends of the fluid storage chamber 210. The self-sealing member 400 is disposed at one side of the liquid storage chamber 210, the self-sealing member 400 includes a protective cover 410, a piston 430 and a pushing member, the pushing member includes a push rod 420 and a spring 440, the protective cover 410 seals an end of the liquid storage chamber 210, the push rod 420 connects the piston 430 with the protective cover 410, the compression spring is disposed between the piston 430 and the protective cover 410, the spring 440 is disposed in a compressed state, the piston 430 has a tendency to squeeze fluid to one side of the sampling section 100, when the sampling section 100 and the liquid storage section 200 relatively move to open or break the sealing structure 500, the spring 440 is released, the spring 440 drives the push rod 420 to drive the piston 430 to move, the piston 430 extrudes the fluid, and pushes the fluid to flow to the sampling section 100, so that the flow speed of the fluid can be improved. The arrangement of the piston 430 and the compression spring 440 can limit the flow direction of the fluid, and the piston 430 abuts against the fluid and has a tendency to push the fluid to flow toward the sampling section 100, so that the sealing effect on the fluid can be improved, and leakage of the fluid during the transportation and use process can be avoided.

In some embodiments, the sampling section 100 is provided with a limit protrusion 120, and the reservoir section 200 is provided with a limit groove 221 that mates with the limit protrusion 120; alternatively, the liquid storage section 200 is provided with a limiting protrusion 120, and the sampling section 100 is provided with a limiting groove 221 matched with the limiting protrusion 120. The sampling section 100 links to each other with depositing the liquid section 200, but sampling section 100 and depositing the relative motion of liquid section 200, set up spacing arch 120 and spacing recess 221 between sampling section 100 and depositing the liquid section 200, can carry out spacingly to the relative motion between sampling section 100 and the liquid section 200, specifically, in some embodiments, sampling section 100 is provided with spacing arch 120, it is provided with spacing recess 221 to deposit the liquid section 200, spacing arch 120 sets up the outer edge at sampling section 100, spacing recess 221 sets up the tip of depositing the liquid section 200, spacing recess 221 butt spacing arch 120, carry out spacingly to sampling section 100, can make the relative motion of sampling section 100 and depositing the liquid section 200 stay in certain extent, avoid the motion of sampling section 100 to surpass the stroke, security and validity when having guaranteed the use. It will be appreciated that in other embodiments, the reservoir section 200 is provided with a limiting protrusion 120, and the sampling section 100 is provided with a limiting groove 221 cooperating with the limiting protrusion 120, and the relative movement between the sampling section 100 and the reservoir section 200 can be limited as well.

In some embodiments, the sampling section 100 is provided with a flared end within which the sampling member 300 is disposed. The horn-shaped end portion can increase the contact area of the end portion, thereby increasing the contact area of the sampling member 300 and improving the sampling effect of the sampling member 300. In some embodiments, the sampling component 300 is adhered to the sampling section 100 by glue, and the connection mode of glue adhesion is simple and stable, so that the connection strength requirement of the sampling component 300 in environment wiping sampling can be met, and the sampling quality is ensured. It should be noted that in some other embodiments, the sampling member 300 may be further fixed to the sampling segment 100 by an interference fit, so that the installation of the sampling cotton can be achieved.

In some embodiments, a protective cover is provided in addition to the sampling member 300, and is used to protect the sampling member 300 from contamination. Further, in some embodiments, a protective cap is mounted to the end of the sampling section 100 by a threaded connection, protecting the sampling member 300 disposed at the end of the sampling section 100. It will be appreciated that the protective cover may also be mounted to the sampling section 100 by means of a snap-fit connection, a mortise-and-tenon fit, or the like.

In some embodiments, sampling member 300 is configured to sample cotton. The sampling cotton is suitable for liquid sampling, solid sampling and other sampling modes, and meets the requirements of biological sample sampling and environment wiping sampling. It will be appreciated that in some other embodiments, foam, cotton, paper, or any other material having water absorbing properties may be used as the material from which the sampling member 300 is made.

In some embodiments, a volume indicator is also disposed between the sampling member 300 and the sampling segment 100. The volume indicator is disposed between the sampling member 300 and the sampling segment 100, monitors the flow rate of fluid flowing into the sampling member 300, changes color when the fluid flowing through the volume indicator reaches a certain level, and can determine whether a sufficient amount of fluid flows into the sampling member 300 to ensure that the sufficient fluid is diluted and mixed with the sample on the sampling member 300.

The following describes in detail the operation of the sampling device according to the first embodiment of the present application:

the sampling device can be used for fluid sampling, diluting the acquired fluid sample and rapidly completing sampling and dilution; the device is also suitable for solid sampling, and the sampling component 300 is pretreated by using fluid before sampling, so that the solid sampling effect is improved, the universality is good, and the application range is wide.

When the sampling device of the first embodiment is used for fluid sampling, the fluid in the reservoir 210 is set as the diluent. First, fluid sampling is performed using the sampling member 300 on the sampling section 100; after sampling, through the relative motion between the sampling section 100 and the liquid storage section 200, the sampling section 100 moves in the accommodating cavity 220, the sampling section 100 can be abutted and the sealing structure 500 is opened, so that the liquid storage cavity 210 is communicated with the hollow flow channel 110 of the sampling section 100, fluid flows to the sampling component 300 through the hollow flow channel 110, the viscosity of the diluted sample is reduced, and smooth dialysis of the sample during detection is ensured.

When the sampling device of the first embodiment is used for powder sampling, the fluid in the reservoir 210 is set as the pretreatment reagent. Before the sampling part 300 performs sampling, firstly, the sampling section 100 and the liquid storage section 200 relatively move, the sampling section 100 moves in the accommodating cavity 220, the sampling section 100 can abut against and open the sealing structure 500, the fluid in the liquid storage cavity 210 flows to the sampling part 300, and the sampling part 300 is preprocessed; then, the powder sampling is performed using the sampling member 300 after the pretreatment, and the sampling effect is better.

Example two

Referring to fig. 4 to 6, a sampling device according to a second embodiment of the present application will be described.

In some embodiments, a connector 600 is disposed between the sampling section 100 and the storage section 200, the connector 600 includes a fixing portion 610 and a connecting section 620, the fixing portion 610 is fixedly mounted on the storage section 200, the connecting section 620 is connected with the opening 241, the sealing structure 500 is disposed on the connecting section 620, and the sampling section 100 is movably mounted in the connecting section 620. The connecting piece 600 connects the sampling section 100 and the liquid storage section 200, specifically, the fixing part 610 is fixedly installed on the liquid storage section 200, so as to realize the connection and fixation of the connecting piece 600 and the liquid storage section 200; the sampling section 100 may be movably mounted in the connection section 620 to achieve a movable connection of the sampling section 100 to the connector 600. The sealing structure 500 is disposed on the connection section 620, and the sealing structure 500 disposed on the connection section 620 can be opened or broken by the movement of the sampling section 100 relative to the connection section 620 to deliver fluid to the sampling member 300. The connecting piece 600 is arranged between the sampling section 100 and the liquid storage section 200, the sampling section 100 and the liquid storage section 200 are sealed and connected by using the connecting piece 600, the matching precision requirement between the sampling section 100 and the liquid storage section 200 can be reduced, and the sealing and connecting effects are better.

In some embodiments, a sealing ring for sealing is further disposed between the sampling section 100 and the connecting section 620, and the sealing ring can prevent fluid from flowing out from a gap between the connecting section 620 and the sampling section 100, so as to enhance sealing effect.

It will be appreciated that in some embodiments, the fixing portion 610 of the connector 600 is fixed to the sampling section 100 and the storage section 200 at the same time, so as to connect the sampling section 100 and the storage section 200. For example, the fixing portion 610 is configured as a sleeve with two open ends, the sealing structure 500 is disposed inside the sleeve, the sampling section 100 and the liquid storage section 200 are sleeved on two sides of the sleeve, at this time, the sampling section 100 includes an outer shell abutting the sleeve on the outer side and an inner tube abutting the sealing member on the inner side, and the hollow flow channel 110 is disposed in the inner tube, so that communication between the sampling section 100 and the liquid storage section 200 can be achieved.

In some embodiments, the sealing structure 500 is a sealing plug disposed on the connection section 620, and the sampling section 100 can push the sealing plug apart, so that the connection section 620 communicates with the fluid storage chamber 210. In other embodiments, the sealing structure 500 may also be provided as a sealing film or as a frangible plastic material, such as a frangible plastic cap or the like, disposed at the junction at the opening 241. In some embodiments, the seal 500 may also be configured as a connection that may be pushed apart or opened or broken in one direction, such as a pinch plate mounted at the opening 241 by a snap-fit connection, or the like. In other embodiments, the sealing structure 500 may be made of a material that is easily pierced or pushed away, such as aluminum foil, plastic, rubber, silicone, and the like.

The following describes the operation of the sampling device according to the second embodiment of the present application in detail:

when the sampling device in the second embodiment is used for fluid sampling, the fluid in the reservoir 210 is set as the diluent. First, fluid sampling is performed using the sampling member 300 on the sampling section 100; after sampling, through the relative motion between the sampling section 100 and the connecting piece 600, the sampling section 100 moves relative to the connecting section 620, and the sampling section 100 can be abutted against and push away the sealing plug, so that the hollow flow channel 110, the connecting section 620 and the liquid storage cavity 210 are communicated, and fluid flows to the sampling component 300 through the hollow flow channel 110, so that the viscosity of a sample is reduced after the sample is diluted, and the smooth dialysis of the sample during detection is ensured.

When the sampling device in the second embodiment is used for powder sampling, the fluid in the reservoir 210 is set as the pretreatment reagent. Before sampling the sampling component 300, firstly, through the relative movement between the sampling section 100 and the connecting piece 600, the sampling section 100 moves relative to the connecting section 620, the sampling section 100 can abut against and push away the sealing plug, the fluid in the liquid storage cavity 210 flows to the sampling component 300, and the sampling component 300 is preprocessed; then, the sampling is performed by using the sampling part 300 after the pretreatment, and the sampling effect is better.

Example III

Referring to fig. 7 to 8, a sampling device according to the present application will be described in a third embodiment.

In some embodiments, the sampling segment 100 and the reservoir segment 200 are movably connected by the engagement of the slot-in-slot buckle 140. Specifically, in some embodiments, the sampling section 100 is provided with a first clamping groove 231 and a second clamping groove 232, the liquid storage section 200 is provided with a buckle 140, and the sampling section 100 and the liquid storage section 200 can move relatively, so that the buckle 140 moves between the first clamping groove 231 and the second clamping groove 232. Referring to fig. 8, the sampling section 100 includes a housing provided with a first clamping groove 231 and a second clamping groove 232, and an inner tube provided with a hollow flow channel 110, when the buckle 140 is located in the first clamping groove 231, the hollow flow channel 110 is separated from the opening 241 by the sealing structure 500, and fluid is stored in the liquid storage cavity, so that the fluid is stored in a sealing manner. When fluid needs to be added to the sampling component 300, the sampling segment 100 and the liquid storage segment 200 move relatively, the buckle 140 is pushed onto the second clamping groove 232, and referring to fig. 8, at this time, the inner tube of the sampling segment 100 pierces the sealing structure 500, the hollow flow channel 110 is communicated with the liquid storage cavity 210, and the fluid can circulate to the sampling component 300 through the hollow flow channel 110. It will be appreciated that in other embodiments, the reservoir section 200 is provided with a first slot 231 and a second slot 232, and the sampling section 100 is provided with a buckle 140, so that the connection between the sampling section 100 and the reservoir section 200 can be fixed and relatively moved.

In some embodiments, the sampling section 100 is further provided with a separable protecting member 130, the liquid storage section 200 is provided with a limiting portion matched with the protecting member 130, and the protecting member 130 abuts against the limiting portion to limit the movement of the buckle 140 from the first clamping groove 231 to the second clamping groove 232. The protector 130 is provided to prevent malfunction and to store and protect the fluid in the reservoir section 200. Specifically, the protecting member 130 is disposed on the sampling section 100, a connecting strip which is easy to open or break is disposed between the protecting member 130 and the sampling section 100, the liquid storage section 200 is provided with a limiting portion, the protecting member 130 is disposed on one side of the limiting portion, and the limiting portion is matched with the protecting member 130, so that the relative movement between the sampling section 100 and the liquid storage section 200 can be limited. When fluid needs to be added to the sampling component 300, the protecting piece 130 is removed from the sampling section 100, the sampling section 100 moves relative to the liquid storage section 200, at this time, the buckle 140 can move from the first clamping groove 231 to the second clamping groove 232, the inner tube of the sampling section 100 pierces the sealing structure 500, the hollow runner 110 is communicated with the liquid storage cavity 210, and the fluid can circulate to the sampling component 300 through the hollow runner 110.

It should be noted that, in some embodiments, the sampling section 100 and the storage section 200 are movably connected through a threaded connection, and the relative movement between the sampling section 100 and the storage section 200 can be also realized. For example, the sampling section 100 includes a housing and an inner tube, the housing of the sampling section 100 is threadably coupled to the reservoir section 200, and movement of the inner tube relative to the seal 500 is achieved by threading in and threading out the sampling section 100, causing the inner tube to pierce the seal 500 and add fluid to the sampling member 300. It will be appreciated that in some embodiments, the sampling section 100 is provided with external threads, the reservoir section 200 is provided with internal threads, and in other embodiments, the sampling section 100 is provided with internal threads, and the reservoir section 200 is provided with external threads, both to enable connection fixation and relative movement between the sampling section 100 and the reservoir section 200.

In some embodiments, the seal 500 is configured as a breakable seal, and the inner tube of the sampling segment 100 is capable of puncturing the seal to place the sampling segment 100 in communication with the fluid reservoir 210. In other embodiments, the sealing structure 500 may also be configured as a sealing plug that can be pushed apart, the position and size of the sealing plug corresponding to the position and size of the inner tube. The sealing structure 500 may also be provided as a plastic material that is easily opened or broken, such as a frangible plastic cap or the like provided at the junction at the opening 241. In some embodiments, the seal 500 may also be configured as a connection that may be pushed apart or opened or broken in one direction, such as a pinch plate mounted at the opening 241 by a snap-fit connection, or the like. In other embodiments, the sealing structure 500 may be made of a material that is easily pierced, such as aluminum foil, plastic, or other materials that are easily pierced.

The following describes the operation of the sampling device according to the third embodiment of the present application in detail:

when the sampling device in the third embodiment is used for fluid sampling, the fluid in the reservoir 210 is set as the diluent. First, fluid sampling is performed using the sampling member 300 on the sampling section 100; after sampling, the protecting piece 130 is removed from the sampling section 100, the sampling section 100 and the liquid storage section 200 move relatively, the buckle 140 is pushed onto the second clamping groove 232 from the first clamping groove 231, at the moment, the inner pipe of the sampling section 100 pierces the sealing structure 500, the hollow flow channel 110 is communicated with the liquid storage cavity 210, and fluid can circulate to the sampling part 300 through the hollow flow channel 110, so that the viscosity of a sample is reduced after the sample is diluted, and smooth dialysis of the sample during detection is ensured.

When the sampling device in the third embodiment is used for solid/powder sampling, the fluid in the reservoir 210 is set as the pretreatment reagent. Before sampling the sampling component 300, firstly, removing the protecting piece 130 from the sampling section 100, relatively moving the sampling section 100 and the liquid storage section 200, pushing the buckle 140 from the first clamping groove 231 to the second clamping groove 232, at this time, the inner pipe of the sampling section 100 pierces the sealing structure 500, the fluid in the liquid storage cavity 210 flows to the sampling component 300, and preprocessing the sampling component 300; then, the sampling is performed by using the sampling part 300 after the pretreatment, and the sampling effect is better.

Example IV

Referring to fig. 9 to 11, a sampling device according to a fourth embodiment of the present application will be described.

In some embodiments, the liquid storage section 200 is provided with a liquid outlet pipe 240, the opening 241 is disposed on a side wall of the liquid outlet pipe 240, the sealing structure 500 is sleeved on the liquid outlet pipe 240 and seals the opening 241, and the sampling section 100 is provided with an abutting part 150 corresponding to the sealing structure 500. It can be understood that the position of the abutting portion 150 corresponds to the position of the sealing structure 500, when the sampling section 100 moves relative to the storage section 200, the abutting portion 150 can abut against the sealing member on the liquid outlet pipe 240 and push the sealing member away from the opening 241, the opening 241 disposed on the sidewall of the liquid outlet pipe 240 is in communication with the hollow pipe of the sampling section 100, and the fluid can be circulated to the sampling member 300 through the hollow flow passage 110.

The following describes the operation of the sampling device according to the fourth embodiment of the present application in detail:

when the sampling device in the fourth embodiment is used for fluid sampling, the fluid in the reservoir 210 is set as the diluent. First, fluid sampling is performed using the sampling member 300 on the sampling section 100; after sampling, the sampling section 100 and the liquid storage section 200 are relatively moved, the abutting part 150 of the sampling section 100 can abut against the sealing component on the liquid outlet pipe 240, the sealing component is pushed away from the opening 241, the opening 241 arranged on the side wall of the liquid outlet pipe 240 is communicated with the hollow pipeline of the sampling section 100, and fluid can be communicated to the sampling component 300 through the hollow runner 110, so that the viscosity of a sample is reduced after the sample is diluted, and the smooth dialysis of the sample during detection is ensured.

When the sampling device in the fourth embodiment is used for solid/powder sampling, the fluid in the reservoir 210 is set as the pretreatment reagent. Before sampling the sampling member 300, first, the sampling section 100 and the liquid storage section 200 are relatively moved, the abutting portion 150 of the sampling section 100 can abut against the sealing member on the liquid outlet pipe 240, the sealing member is pushed away from the opening 241, the opening 241 provided on the side wall of the liquid outlet pipe 240 is communicated with the hollow pipe of the sampling section 100, the fluid in the liquid storage chamber 210 flows to the sampling section 300, and the sampling section 300 is pretreated; then, the sampling is performed by using the sampling part 300 after the pretreatment, and the sampling effect is better.

Example five

Referring to fig. 1-3 and fig. 12-13, a detailed description will be given below of the detection and analysis operation procedure of the integrated detection reagent card for sampling and storing liquid according to the embodiment of the present application:

the detection reagent card integrating sampling and liquid storage comprises a sampling device and a lateral flow chromatography device 700, wherein the lateral flow chromatography device 700 is connected with the sampling device in a plug-in mode; the sampling device is used for sampling and storing fluid. The lateral flow chromatography device 700 comprises a shell and a detection test strip 730, wherein the detection test strip 730 is positioned inside the shell, the shell is provided with a sample inlet 721, the sampling device is inserted into the sample inlet 721 through a sampling component and connected with the lateral flow chromatography device, a sample channel 722 is arranged between the sample inlet and the detection test strip 730, and a sample flows to the detection test strip 730 through the sample channel.

The lateral flow chromatography detection apparatus 700 includes an upper housing 710, a lower housing 720, and a test strip 730, where the test strip 730 is located in a space where the upper housing 710 and the lower housing 720 are folded, and the upper housing 710 is provided with an observation port 711. The specific model of the lateral flow chromatography detection device is not limited, and can be a common drop hole card type in the market.

When fluid sampling is carried out:

When the integrated detection reagent card for sampling and storing liquid is used for liquid sampling, the fluid in the liquid storing cavity 210 is set as diluent:

1) First, sampling is performed using a sampling device: sampling is performed by the sampling unit 300 on the sampling section 100;

2) After sampling, the sampling member 300 is inserted into the sample inlet 721 to connect the sampling device with the lateral flow chromatography device 700;

3) The sample is then transferred from the sampling member 300 to the lateral flow assay device 700 by mechanical compression and release of the fluid stored in the sampling device;

4) Sample enters the lateral flow chromatography device 700 from the sample inlet 721, and then flows to the detection test strip 730 through the sample channel 722 to start chromatography sample loading;

5) The detection result is interpreted by visually or by optical means to identify the detection condition on the test strip 730.

The fluid release process in the sampling device is specifically as follows: through the relative motion between the sampling section 100 and the liquid storage section 200, the sampling section 100 moves in the accommodating cavity 220 of the liquid storage section 200, the sampling section 100 can be abutted against and open the sealing structure 500, so that the liquid storage cavity 210 is communicated with the hollow flow channel 110 of the sampling section 100, fluid flows to the sampling component 300 through the hollow flow channel 110, the viscosity of a sample is reduced after the sample is released by the fluid in the liquid storage cavity 210 of the sampling device, and dialysis can be smoothly performed during detection.

(II) powder sampling:

when the sample-reservoir integrated test reagent card is used for powder sampling, the fluid in the reservoir 210 is set as the pretreatment reagent. Before the sampling part 300 performs sampling, firstly, the sampling section 100 and the liquid storage section 200 relatively move, the sampling section 100 moves in the accommodating cavity 220, the sampling section 100 can abut against and open the sealing structure 500, the fluid in the liquid storage cavity 210 flows to the sampling part 300, and the sampling part 300 is preprocessed; then, the powder sampling is performed using the sampling member 300 after the pretreatment, and the sampling effect is better.

1) Firstly, releasing pretreatment reagent, relatively moving the sampling section 100 and the liquid storage section 200, moving the sampling section 100 in the accommodating cavity 220, enabling the sampling section 100 to abut against and open the sealing structure 500, enabling the fluid in the liquid storage cavity 210 to flow to the sampling component 300, and pretreating (or wetting) the sampling component 300;

2) Sampling using a sampling device: sampling is performed by the sampling unit 300 on the sampling section 100;

3) After sampling, the sampling member 300 is inserted into the sample inlet 721 to connect the sampling device with the lateral flow chromatography device 700;

4) The sample is then transferred from the sampling member 300 to the lateral flow assay device 700 by mechanical compression;

5) Sample enters the lateral flow chromatography device 700 from the sample inlet 721, and then flows to the detection test strip 730 through the sample channel 722 to start chromatography sample loading;

6) The detection result is interpreted by visually or by optical means to identify the detection condition on the test strip 730.

Of course, in some powder sampling scenarios without pretreatment, powder sampling may be performed first, the powder sample is adsorbed by using electrostatic attraction force, then the sampling unit 300 is inserted into the sample inlet 721, the fluid in the liquid storage chamber 210 is triggered to be released, the powder sample is flushed out of the sampling unit 300 by the thrust of the fluid, and flows to the test strip 730 through the sample channel 722, so as to start chromatographic loading.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of one of ordinary skill in the art without departing from the spirit of the present invention.

Claims (10)

1. The utility model provides a sample exists detection reagent card of liquid integration which characterized in that includes: the lateral flow chromatography device is connected with the sampling device in a plug-in mode;

the sampling device is used for sampling and storing fluid, and comprises:

The sampling section is provided with a sampling component, and a hollow runner connected with the sampling component is arranged in the sampling section;

the liquid storage section is provided with a liquid storage cavity for storing the fluid, and the liquid storage cavity is provided with an opening for the fluid to flow out;

the liquid storage device comprises a liquid storage section, a sampling section and a liquid storage section, wherein a sealing structure used for blocking the hollow flow channel from being communicated with the opening is arranged between the sampling section and the liquid storage section, and the sampling section and the liquid storage section can move relatively so as to open or destroy the sealing structure.

2. The integrated sampling and fluid storage detection reagent card of claim 1, wherein the fluid storage section is further provided with a containing cavity, the sealing structure is arranged between the containing cavity and the fluid storage cavity, and the sampling section is movably installed in the containing cavity.

3. The integrated sampling and fluid storage detection reagent card according to claim 1, wherein the sampling section is connected with the fluid storage section through a connecting piece, the connecting piece comprises a fixing part and a connecting section, the fixing part is installed on the fluid storage section, the sampling section is movably installed in the connecting section, the fluid storage cavity is connected with the sampling section through the connecting section, and the sealing structure seals an opening communicated with the fluid storage cavity on the connecting section.

4. The integrated detection reagent card for sampling and storing liquid according to claim 1, wherein the sampling section is provided with a first clamping groove and a second clamping groove, the storing liquid section is provided with a buckle, and the sampling section and the storing liquid section are movably connected through the matching of the clamping groove buckle;

when the buckle is positioned in the first clamping groove, the sealing structure cuts off the hollow runner from the opening;

when the buckle is positioned in the second clamping groove, the sealing structure is opened or destroyed, and the hollow runner is communicated with the opening.

5. The integrated sampling and fluid storage detection reagent card of claim 4, wherein the sampling section is further provided with a separable protection member, the fluid storage section is provided with a limiting portion matched with the protection member, and the protection member abuts against the limiting portion to limit the movement of the buckle from the first clamping groove to the second clamping groove.

6. The integrated sampling and storage detection reagent card of claim 1, wherein the storage section is provided with a liquid outlet pipe, the opening is formed in the side wall of the liquid outlet pipe, the sealing structure is movably sleeved on the liquid outlet pipe and seals the opening, and the sampling section is provided with an abutting part corresponding to the sealing structure.

7. The integrated sample and reservoir test reagent card of claim 1, wherein the reservoir is further provided with a self-sealing member comprising a protective cover disposed on a side of the reservoir remote from the sampling section, a piston and a push member, the protective cover sealing one side of the reservoir, the piston being connected to the protective cover by the push member, the push member being disposed between the piston and the protective cover.

8. The integrated sampling and storage detection reagent card according to claim 1, wherein the sampling section is provided with a limit protrusion, and the storage section is provided with a limit groove matched with the limit protrusion; or, the liquid storage section is provided with a limiting protrusion, and the sampling section is provided with a limiting groove matched with the limiting protrusion.

9. The integrated test reagent card of claim 1, wherein a volume indicator is further disposed between the sampling member and the sampling segment.

10. The integrated sampling and fluid storage detection reagent card of claim 1, wherein the lateral flow chromatography device comprises a shell and a detection test strip, the detection test strip is positioned inside the shell, the shell is provided with a sample inlet, the sampling device is inserted into the sample inlet through the sampling component to be connected with the lateral flow chromatography device, a sample channel is arranged between the sample inlet and the detection test strip, and the sample flows to the detection test strip through the sample channel.