CN117187228A - Catalase preservative and preparation method thereof - Google Patents
Catalase preservative and preparation method thereof Download PDFInfo
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- CN117187228A CN117187228A CN202311180395.3A CN202311180395A CN117187228A CN 117187228 A CN117187228 A CN 117187228A CN 202311180395 A CN202311180395 A CN 202311180395A CN 117187228 A CN117187228 A CN 117187228A
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- buffer solution
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- catalase
- preservative
- pure water
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- 102000016938 Catalase Human genes 0.000 title claims abstract description 89
- 108010053835 Catalase Proteins 0.000 title claims abstract description 89
- 230000002335 preservative effect Effects 0.000 title claims abstract description 77
- 239000003755 preservative agent Substances 0.000 title claims abstract description 76
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 239000007853 buffer solution Substances 0.000 claims abstract description 154
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims abstract description 39
- 235000018417 cysteine Nutrition 0.000 claims abstract description 39
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 29
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 29
- 238000002156 mixing Methods 0.000 claims abstract description 26
- 239000002994 raw material Substances 0.000 claims abstract description 17
- 238000003756 stirring Methods 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 66
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 46
- 239000011780 sodium chloride Substances 0.000 claims description 23
- PYMYPHUHKUWMLA-UHFFFAOYSA-N 2,3,4,5-tetrahydroxypentanal Chemical compound OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 22
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 claims description 22
- BNIAKAQSIZOVSN-UHFFFAOYSA-N [Na].CC(O)CO Chemical compound [Na].CC(O)CO BNIAKAQSIZOVSN-UHFFFAOYSA-N 0.000 claims description 22
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 22
- ZFSLODLOARCGLH-UHFFFAOYSA-N isocyanuric acid Chemical compound OC1=NC(O)=NC(O)=N1 ZFSLODLOARCGLH-UHFFFAOYSA-N 0.000 claims description 22
- 229920000136 polysorbate Polymers 0.000 claims description 22
- 235000010413 sodium alginate Nutrition 0.000 claims description 22
- 239000000661 sodium alginate Substances 0.000 claims description 22
- 229940005550 sodium alginate Drugs 0.000 claims description 22
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 claims description 22
- ODLHGICHYURWBS-LKONHMLTSA-N trappsol cyclo Chemical compound CC(O)COC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)COCC(O)C)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1COCC(C)O ODLHGICHYURWBS-LKONHMLTSA-N 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 12
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 11
- 239000003814 drug Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 239000000853 adhesive Substances 0.000 claims description 5
- 230000001070 adhesive effect Effects 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 abstract description 7
- 108090000790 Enzymes Proteins 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 238000003860 storage Methods 0.000 abstract description 6
- 230000015556 catabolic process Effects 0.000 abstract description 3
- 238000006731 degradation reaction Methods 0.000 abstract description 3
- 239000000872 buffer Substances 0.000 abstract 2
- 230000000052 comparative effect Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 238000000605 extraction Methods 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000004973 liquid crystal related substance Substances 0.000 description 2
- 239000004302 potassium sorbate Substances 0.000 description 2
- 235000010241 potassium sorbate Nutrition 0.000 description 2
- 229940069338 potassium sorbate Drugs 0.000 description 2
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 2
- 235000010234 sodium benzoate Nutrition 0.000 description 2
- 239000004299 sodium benzoate Substances 0.000 description 2
- 239000004753 textile Substances 0.000 description 2
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 description 1
- 108010053775 Nisin Proteins 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical group N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000004309 nisin Substances 0.000 description 1
- 235000010297 nisin Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The application discloses a catalase preservative and a preparation method thereof, belonging to the technical field of biological products. Wherein the catalase preservative comprises the following raw materials in parts by weight: buffer one: 20-40 parts of buffer II: 20-40 parts of buffer solution III: 20-40 parts of cysteine: 3-6 parts of polyvinyl alcohol: 5-10 parts. Wherein the preparation method of the catalase comprises the following steps: preparing a first buffer solution, a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution; cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature. After 180d of storage at normal temperature, the degradation force of the enzyme can be kept unchanged, so that the storage cost of enterprises is greatly reduced, and the operation control conditions of the enterprises in production are eased.
Description
Technical Field
The application belongs to the technical field of biological products, and particularly relates to an improved catalase preservative.
Background
Catalase (CAT) is an important biological enzyme preparation product, is widely applied to biological deoxidation and the like after textile oxygen bleaching in textile industry, and can improve dyeing quality and reduce wastewater discharge in food industry, rubber industry and the like.
The catalase is generally produced by biological fermentation or extracted from animal livers, and the catalase production in China is still common in the extraction method from animal livers at present, and the fermentation method is still in the experimental trial production stage. In the process of extracting catalase from animal livers, due to pollution, environmental control and other reasons, a preservative is required to be added into a produced catalase liquid preparation, namely a green enzyme liquid for preservative treatment, potassium sorbate is usually added, sodium benzoate is used as the preservative of the catalase to maintain CAT activity, and the potassium sorbate is added, so that the sodium benzoate has a certain preservative effect, but has a limited preservative effect under certain conditions such as a neutral condition, and the catalase liquid preparation is extremely easy to precipitate, deactivate and the like along with the extension of time.
The method for preserving and preserving food grade catalase of Chinese patent application No. 20610038235.5 is characterized in that the existing CAT preservative is improved, sodium chloride, nisin and nipagin complex grease are added into catalase clear enzyme liquid which is not preserved in the production process of an extraction method to serve as a preserving and mildew-proof agent of catalase CAT, but the production and use cost is high, the preserving effect is general, the storage time of the preserved CAT product is short, the enzyme activity of the preserved CAT product is not high, and the effect is not ideal as the method for preparing catalase by an extraction method of Chinese patent No. ZL03152957.7, which is characterized in that 300000 units of penicillin is added into soaking liquid to serve as the preservative.
Disclosure of Invention
1. Problems to be solved
Aiming at the problems in the prior art, the application provides the catalase preservative and the preparation method thereof, and the degradation force of the catalase preservative can be kept unchanged after the catalase preservative is stored for 180 days at normal temperature, so that the storage cost of enterprises is greatly reduced, and the operation control conditions of the enterprises in production are eased.
2. Technical proposal
In order to solve the problems, the application adopts the following technical scheme.
The catalase preservative comprises the following raw materials in parts by weight:
20-40 parts of buffer solution I,
20-40 parts of buffer solution II,
three 20-40 parts of buffer solution,
3-6 parts of cysteine,
5-10 parts of polyvinyl alcohol.
The catalase preservative described above,
the adhesive comprises the following raw materials in parts by weight:
25-35 parts of buffer solution one,
25-30 parts of buffer solution II,
30-40 parts of buffer solution three,
3-6 parts of cysteine,
5-10 parts of polyvinyl alcohol.
The catalase preservative described above,
the adhesive comprises the following raw materials in parts by weight:
one 30 parts of buffer solution is used for preparing the liquid medicine,
28 parts of a second buffer solution, namely,
three 35 parts of buffer solution are used for preparing the buffer solution,
5 parts of cysteine, namely, 5 parts of cysteine,
8 parts of polyvinyl alcohol.
The catalase preservative described above,
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
the catalase preservative described above,
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
the catalase preservative described above,
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
a method for preparing a catalase preservative, comprising the steps of:
preparing a first buffer solution, a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
3. Advantageous effects
Compared with the prior art, the application has the beneficial effects that:
the application has extremely simple formula, three groups of buffer solutions are arranged, cysteine and polyvinyl alcohol are matched, and a small amount of solution is used to reduce the use of chemical reagents, thereby protecting the environment better, and being an important index considered by the inventor when the formula is researched and developed; meanwhile, the preservative can preserve the catalase for 180 days at normal temperature, and the residual rate is maintained above 85%.
Detailed Description
The application is further described below in connection with specific embodiments.
Example 1
The catalase preservative comprises the following raw materials in parts by weight:
20 parts of a buffer solution, namely, a liquid crystal,
40 parts of a second buffer solution, namely,
three 20 parts of buffer solution are used for preparing the buffer solution,
6 parts of cysteine, namely, 6 parts of cysteine,
5 parts of polyvinyl alcohol.
The catalase preservative described above,
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
the catalase preservative described above,
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
the catalase preservative described above,
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
the preparation method of the catalase preservative comprises the following steps:
preparing a first buffer solution, a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
Example 2
The catalase preservative comprises the following raw materials in parts by weight:
one part of the buffer solution is 40 parts,
20 parts of a second buffer solution, namely,
three 40 parts of buffer solution are used for preparing the buffer solution,
3 parts of cysteine, namely, 3 parts of cysteine,
10 parts of polyvinyl alcohol.
The catalase preservative described above,
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
the catalase preservative described above,
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
the catalase preservative described above,
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
the preparation method of the catalase preservative comprises the following steps:
preparing a first buffer solution, a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
Example 3
The catalase preservative comprises the following raw materials in parts by weight:
25 parts of buffer solution, namely, one part of buffer solution,
30 parts of a second buffer solution, namely,
three 30 parts of buffer solution are used for preparing the buffer solution,
6 parts of cysteine, namely, 6 parts of cysteine,
5 parts of polyvinyl alcohol.
The catalase preservative described above,
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
the catalase preservative described above,
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
the catalase preservative described above,
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
the preparation method of the catalase preservative comprises the following steps:
preparing a first buffer solution, a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
Example 4
The catalase preservative comprises the following raw materials in parts by weight:
35 parts of a buffer solution, namely, a liquid crystal,
25 parts of a second buffer solution, namely,
three 40 parts of buffer solution are used for preparing the buffer solution,
3 parts of cysteine, namely, 3 parts of cysteine,
10 parts of polyvinyl alcohol.
The catalase preservative described above,
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
the catalase preservative described above,
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
the catalase preservative described above,
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
the preparation method of the catalase preservative comprises the following steps:
preparing a first buffer solution, a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
Example 5
The catalase preservative comprises the following raw materials in parts by weight:
one 30 parts of buffer solution is used for preparing the liquid medicine,
28 parts of a second buffer solution, namely,
three 35 parts of buffer solution are used for preparing the buffer solution,
5 parts of cysteine, namely, 5 parts of cysteine,
8 parts of polyvinyl alcohol.
The catalase preservative described above,
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
the catalase preservative described above,
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
the catalase preservative described above,
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
the preparation method of the catalase preservative comprises the following steps:
preparing a first buffer solution, a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
Comparative example 1
The catalase preservative comprises the following raw materials in parts by weight:
one 30 parts of buffer solution is used for preparing the liquid medicine,
28 parts of a second buffer solution, namely,
three 35 parts of buffer solution are used for preparing the buffer solution,
8 parts of polyvinyl alcohol.
The catalase preservative described above,
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
the catalase preservative described above,
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
the catalase preservative described above,
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
the preparation method of the catalase preservative comprises the following steps:
preparing a first buffer solution, a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
polyvinyl alcohol is prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
Comparative example 2
The catalase preservative comprises the following raw materials in parts by weight:
one 30 parts of buffer solution is used for preparing the liquid medicine,
28 parts of a second buffer solution, namely,
three 35 parts of buffer solution are used for preparing the buffer solution,
5 parts of cysteine.
The catalase preservative described above,
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
the catalase preservative described above,
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
the catalase preservative described above,
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
the preparation method of the catalase preservative comprises the following steps:
preparing a first buffer solution, a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine is prepared, fully mixed with the mixed buffer solution and homogenized, aseptically canned and refrigerated at low temperature.
Comparative example 3
The catalase preservative comprises the following raw materials in parts by weight:
28 parts of a second buffer solution, namely,
three 35 parts of buffer solution are used for preparing the buffer solution,
5 parts of cysteine, namely, 5 parts of cysteine,
8 parts of polyvinyl alcohol.
The catalase preservative described above,
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
the catalase preservative described above,
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
the preparation method of the catalase preservative comprises the following steps:
preparing a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
Comparative example 4
The catalase preservative comprises the following raw materials in parts by weight:
one 30 parts of buffer solution is used for preparing the liquid medicine,
three 35 parts of buffer solution are used for preparing the buffer solution,
5 parts of cysteine, namely, 5 parts of cysteine,
8 parts of polyvinyl alcohol.
The catalase preservative described above,
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
the catalase preservative described above,
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
the preparation method of the catalase preservative comprises the following steps:
preparing a first buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
Comparative example 5
The catalase preservative comprises the following raw materials in parts by weight:
one 30 parts of buffer solution is used for preparing the liquid medicine,
28 parts of a second buffer solution, namely,
5 parts of cysteine, namely, 5 parts of cysteine,
8 parts of polyvinyl alcohol.
The catalase preservative described above,
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
the catalase preservative described above,
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
the preparation method of the catalase preservative comprises the following steps:
preparing a first buffer solution and a second buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
Test scheme:
in use, the catalase preservatives prepared in examples 1 to 5 and comparative examples 1 to 5 were combined with catalase in an amount of 15:1, storing for 180d at normal temperature, and finally detecting the enzyme activity.
The results show that the test results for examples 1-5 of the present application are as follows: 85%, 86%, 89%, 91%, 92%;
the test results of comparative examples 1 to 5 are as follows: 74%, 72%, 47%, 42%, 46%.
Therefore, after 180d of storage at normal temperature, the degradation force of the enzyme can be kept unchanged, so that the storage cost of enterprises is greatly reduced, and the operation control conditions of the enterprises in production are eased.
The foregoing is a further elaboration of the present application in connection with the detailed description, and it is not intended that the application be limited to the specific embodiments shown, but rather that a number of simple deductions or substitutions be made by one of ordinary skill in the art without departing from the spirit of the application, should be considered as falling within the scope of the application as defined in the appended claims.
Claims (7)
1. A catalase preservative, characterized in that:
the adhesive comprises the following raw materials in parts by weight:
20-40 parts of buffer solution I,
20-40 parts of buffer solution II,
three 20-40 parts of buffer solution,
3-6 parts of cysteine,
5-10 parts of polyvinyl alcohol.
2. The catalase preservative according to claim 1, wherein:
the adhesive comprises the following raw materials in parts by weight:
25-35 parts of buffer solution one,
25-30 parts of buffer solution II,
30-40 parts of buffer solution three,
3-6 parts of cysteine,
5-10 parts of polyvinyl alcohol.
3. The catalase preservative according to claim 2, wherein:
the adhesive comprises the following raw materials in parts by weight:
one 30 parts of buffer solution is used for preparing the liquid medicine,
28 parts of a second buffer solution, namely,
three 35 parts of buffer solution are used for preparing the buffer solution,
5 parts of cysteine, namely, 5 parts of cysteine,
8 parts of polyvinyl alcohol.
4. A catalase preservative according to claim 3, characterised in that:
the buffer solution I comprises thiosulfate, sulfurous acid, cyanuric acid, tween and pure water;
the mass ratio of the thiosulfate to the sulfurous acid to the cyanuric acid to the tween to the pure water is 5:10:4:20:200.
5. a catalase preservative according to claim 3, characterised in that:
the buffer solution comprises proclin950, 2-hydroxypropyl-beta-cyclodextrin, high methoxyl pectin, sodium chloride and pure water;
the mass ratio of proclin950 to 2-hydroxypropyl-beta-cyclodextrin to high methoxyl pectin to sodium chloride to pure water is 1:15:10:20:150.
6. a catalase preservative according to claim 3, characterised in that:
the buffer solution III comprises histidine, propylene glycol sodium alginate and pure water;
the mass ratio of histidine to propylene glycol sodium alginate to pure water is 2:8:100.
7. a preparation method of a catalase preservative is characterized by comprising the following steps of:
the method comprises the following steps:
preparing a first buffer solution, a second buffer solution and a third buffer solution in a sterile mixing chamber, and uniformly stirring and mixing at 4 ℃ to obtain a mixed buffer solution;
cysteine and polyvinyl alcohol are prepared, fully mixed with the mixed buffer solution, homogenized, aseptically canned and refrigerated at low temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311180395.3A CN117187228A (en) | 2023-09-13 | 2023-09-13 | Catalase preservative and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311180395.3A CN117187228A (en) | 2023-09-13 | 2023-09-13 | Catalase preservative and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117187228A true CN117187228A (en) | 2023-12-08 |
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ID=88988396
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| Application Number | Title | Priority Date | Filing Date |
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| CN202311180395.3A Pending CN117187228A (en) | 2023-09-13 | 2023-09-13 | Catalase preservative and preparation method thereof |
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| Country | Link |
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| CN (1) | CN117187228A (en) |
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2023
- 2023-09-13 CN CN202311180395.3A patent/CN117187228A/en active Pending
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