CN113817190B - Degradable double-layer polysaccharide active membrane and preparation method thereof - Google Patents
Degradable double-layer polysaccharide active membrane and preparation method thereof Download PDFInfo
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- CN113817190B CN113817190B CN202111190596.2A CN202111190596A CN113817190B CN 113817190 B CN113817190 B CN 113817190B CN 202111190596 A CN202111190596 A CN 202111190596A CN 113817190 B CN113817190 B CN 113817190B
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D65/00—Wrappers or flexible covers; Packaging materials of special type or form
- B65D65/38—Packaging materials of special type or form
- B65D65/46—Applications of disintegrable, dissolvable or edible materials
- B65D65/466—Bio- or photodegradable packaging materials
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/04—Alginic acid; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/16—Cyclodextrin; Derivatives thereof
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
Abstract
The invention discloses a degradable double-layer polysaccharide active membrane and a preparation method thereof, which comprises the steps of respectively dissolving sodium alginate and chitosan in water and 1% acetic acid aqueous solution, adding glycerol and dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound into the sodium alginate solution and the chitosan solution, uniformly stirring, standing and degassing to respectively obtain a sodium alginate-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound membrane forming solution I and a chitosan-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound membrane forming solution II. And pouring the film forming solution I and the film forming solution II into a film forming container in sequence, and drying to obtain the degradable double-layer polysaccharide active film. The degradable double-layer polysaccharide active film prepared by adopting the raw materials and the process can effectively solve the technical problem that the traditional packaging material cannot be degraded, and meanwhile, the raw materials are wide in source and the preparation process is simple; the obtained composite membrane has smooth, uniform and compact surface, good mechanical property and antibacterial activity, and good application prospect.
Description
Technical Field
The invention belongs to the field of food packaging films, and discloses a preparation method of a degradable double-layer polysaccharide active film and the degradable double-layer polysaccharide active film prepared by the preparation method.
Background
The food package is used as an important means for storing and preserving food, can effectively reduce food spoilage, guarantee food safety and prolong food shelf life. Polymer materials from petrochemical sources have the advantages of good mechanical properties, good barrier properties, low cost and the like, and have been widely applied to food packaging. However, the conventional synthetic polymer materials are difficult to degrade, and a large amount of waste food packaging materials cause serious white pollution to the environment. Meanwhile, petroleum is a non-renewable resource, and the global limited petroleum reserves will face exhaustion at all times. The green sustainability is the basic concept of the modern social and economic development of China and also the main trend of the development of the food packaging industry. Therefore, the development of a novel packaging material which is safe, environment-friendly, degradable and recyclable, in particular an active packaging material which can effectively prevent food spoilage, has important scientific significance for the production and life of human beings.
Biopolymers, which are fourth generation packaging materials, have biodegradability, processability, and no contamination to foods, and are widely favored by researchers. The chitosan has good film forming property, can effectively block gases such as carbon dioxide, oxygen and the like, and is a good film forming substrate. However, a single chitosan film has poor water vapor barrier property and weak mechanical property, and the bacteriostatic and fresh-keeping effects are not obvious, so that the wide application of the chitosan film in the field of packaging is limited. The multilayer film assembly technology utilizes the interaction between multivalent molecules and macromolecules, and can improve the moisture resistance of a single film material. Meanwhile, the added antibacterial active compound can increase the strength of the membrane through the crosslinking action of the membrane matrix and the compound, and improve the antibacterial activity of the membrane material.
Based on the analysis, the invention provides a degradable double-layer polysaccharide active film and a preparation method thereof, and the film has biodegradability, good mechanical properties and bacteriostatic activity, and has wide application prospects in the field of food storage and preservation.
Disclosure of Invention
The invention aims to provide a degradable double-layer polysaccharide active membrane and a preparation method thereof. The active film prepared by the invention has wide raw material source, low cost and good mechanical property, and effectively inhibits the growth of spoilage microorganisms through the release of active substances, thereby achieving the purposes of food preservation and prolonging shelf life. In order to achieve the purpose, the invention is realized by the following technical scheme:
a preparation method of a degradable double-layer polysaccharide active membrane comprises the following steps:
preparing a sodium alginate-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film forming solution I;
preparing a chitosan-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film forming solution II; and
and compounding the film forming solution I and the film forming solution II to obtain the degradable double-layer polysaccharide active film.
Further, the sodium alginate-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film forming solution I is prepared by the following method:
adding sodium alginate into pure water, and stirring in a water bath at 50-60 ℃ for 2-4 h;
adding glycerol and stirring for 1-2 h; and
adding the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, and stirring for 10-30 min to obtain the product.
Further, the mass concentration of the sodium alginate and the chitosan is 1-2 wt%;
the mass concentration of the glycerol is 0.5-1 wt%;
the mass concentration of the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound is 0.05-0.3 wt%.
The chitosan-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film forming solution II is prepared by the following method:
further, adding chitosan into 1% (v/v) acetic acid water solution, and stirring in a water bath at 50-60 ℃ for 2-4 h;
adding glycerol and stirring for 1-2 h; and
adding the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, and stirring for 10-30 min to obtain the product.
Further, the mass concentration of the sodium alginate and the chitosan is 1-2 wt%;
the mass concentration of the glycerol is 0.5-1 wt%;
the mass concentration of the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound is 0.05-0.3 wt%.
Further, the degradable double-layer polysaccharide active membrane is prepared by the following method:
pouring the film forming solution I into a film forming container, and drying for 3-4 h at 50-60 ℃;
pouring the film forming solution II, and continuously drying for 4-6 h; and
and (5) uncovering the film to obtain the film.
Further, the mass ratio of the sodium alginate in the film forming solution I to the chitosan in the film forming solution II is 1: 1.
Further, the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound is prepared by the following method:
dissolving hydroxypropyl-beta-cyclodextrin in pure water, and continuously stirring at 45-50 ℃;
slowly adding a dihydromyricetin ethanol solution, and continuously stirring for reaction for 4-8 h; and
and (5) freeze drying to obtain the product.
Further, the molar mass ratio of the dihydromyricetin to the hydroxypropyl-beta-cyclodextrin is 1: 1.
The invention also discloses a degradable double-layer polysaccharide active membrane prepared by any one of the preparation methods.
Compared with the prior art, the invention has the following advantages:
the invention provides a degradable double-layer polysaccharide active membrane and a preparation method thereof. The natural flavonoid compound dihydromyricetin has antibacterial, antioxidant and various health effects, and is added with dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, so that the dihydromyricetin is slowly released from the film and exerts the biological activity thereof, and the dihydromyricetin and chitosan are synergistic to enhance the antibacterial effect, and the efficacy of the composite film in food preservation can be obviously improved. Meanwhile, the double-layer active composite film has good mechanical property, degradability, health, environmental protection and no pollution, and can be used as an inner packaging material of food.
Detailed Description
The present invention is further described with reference to the following specific examples, which are not intended to limit the invention in any way, and those skilled in the art may make modifications and adaptations of the invention in accordance with the teachings herein.
Example 1
(1) Preparing a dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound: 3.08g of hydroxypropyl-. beta. -cyclodextrin was dissolved in 200mL of pure water, and stirred continuously at 45 ℃. Dissolving 0.64g of dihydromyricetin in 3mL of ethanol, slowly adding the hydroxypropyl-beta-cyclodextrin solution, continuously stirring for reacting for 6 hours, and freeze-drying to obtain the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound.
(2) Preparing a film forming solution: adding 4g of sodium alginate into 200mL of pure water, stirring for 4h in a water bath at 60 ℃, adding 2g of glycerol, continuing to stir for 2h, adding 0.4g of dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, and continuing to stir for 20min to obtain a sodium alginate-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film-forming solution I. Adding 4g of chitosan into 200mL of 1% (v/v) acetic acid aqueous solution, stirring for 4h in a water bath at 60 ℃, adding 1g of glycerol, continuing stirring for 2h, adding 0.4g of dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, and continuing stirring for 20min to obtain a chitosan-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film-forming solution II.
(3) And (3) pouring 15mL of the film forming solution I obtained in the step (2) into a film forming container (phi is 9cm), drying at 50 ℃ for 3h, then pouring 15mL of the film forming solution II, continuously drying for 4h, and removing the film to obtain the degradable double-layer polysaccharide active film.
Example 2
(1) Preparing a dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound: 1.54g of hydroxypropyl-. beta. -cyclodextrin was dissolved in 100mL of purified water and stirred continuously at 50 ℃. Dissolving 0.32g of dihydromyricetin in 1mL of ethanol, slowly adding the hydroxypropyl-beta-cyclodextrin solution, continuously stirring for reacting for 4 hours, and freeze-drying to obtain a dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound.
(2) Preparing a film forming solution: adding 2g of sodium alginate into 100mL of pure water, stirring in a water bath at 55 ℃ for 3h, adding 1g of glycerol, continuing to stir for 1h, adding 0.1g of dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, and continuing to stir for 10min to obtain a sodium alginate-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film-forming solution I. Adding 2g of chitosan into 100mL of 1% (v/v) acetic acid aqueous solution, stirring for 3h in a water bath at 55 ℃, adding 1g of glycerol, continuing to stir for 1h, adding 0.1g of dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, and continuing to stir for 10min to obtain a chitosan-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film-forming solution II.
(3) And (3) pouring 20mL of the film forming solution I obtained in the step (2) into a film forming container (phi is 9cm), drying at 55 ℃ for 4h, then pouring 20mL of the film forming solution II, continuously drying for 5h, and removing the film to obtain the degradable double-layer polysaccharide active film.
Example 3
(1) Preparing a dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound: 2.31g of hydroxypropyl-. beta. -cyclodextrin was dissolved in 150mL of pure water, and stirring was continued at 45 ℃. Dissolving 0.43g of dihydromyricetin in 2mL of ethanol, slowly adding the hydroxypropyl-beta-cyclodextrin solution, continuously stirring for reacting for 8 hours, and freeze-drying to obtain a dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound.
(2) Preparing a film forming solution: adding 3g of sodium alginate into 150mL of pure water, stirring for 2h in a water bath at 60 ℃, adding 1.5g of glycerol, continuing to stir for 1.5h, adding 0.45g of dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, and continuing to stir for 30min to obtain a sodium alginate-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film-forming solution I. Adding 3g of chitosan into 150mL of 1% (v/v) acetic acid aqueous solution, stirring for 2h in a water bath at 60 ℃, adding 1g of glycerol, continuing to stir for 1.5h, adding 0.45g of dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, and continuing to stir for 30min to obtain a chitosan-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film-forming solution II.
(3) And (3) pouring 20mL of the film forming solution I obtained in the step (2) into a film forming container (phi is 9cm), drying at 60 ℃ for 3h, then pouring 20mL of the film forming solution II, continuously drying for 4h, and removing the film to obtain the degradable double-layer polysaccharide active film.
Comparative example 1
A double-layer polysaccharide composite membrane without adding dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound is prepared by the following steps:
(1) preparing a film forming solution: adding 2g of sodium alginate into 100mL of pure water, stirring for 4h in a water bath at 60 ℃, adding 1g of glycerol, and continuously stirring for 2h to obtain a sodium alginate film forming solution I. Adding 2g of chitosan into 100mL of 1% (v/v) acetic acid aqueous solution, stirring for 4h in a water bath at 60 ℃, adding 1g of glycerol, and continuing stirring for 2h to obtain a chitosan film forming solution II.
(2) And (2) pouring 20mL of the film forming solution I obtained in the step (1) into a film forming container (phi is 9cm), drying at 50 ℃ for 4h, pouring 20mL of the film forming solution II, continuously drying for 4h, and removing the film.
Comparative example 2
A double-layer polysaccharide composite membrane added with dihydromyricetin is prepared by the following steps:
(1) preparing a film forming solution: adding 3g of sodium alginate into 150mL of pure water, stirring in a water bath at 55 ℃ for 3h, adding 1.5g of glycerol, continuing to stir for 1h, adding 0.0258g of dihydromyricetin, and continuing to stir for 10min to obtain a sodium alginate-dihydromyricetin film-forming solution I. Adding 3g of chitosan into 150mL of 1% (v/v) acetic acid aqueous solution, stirring in a water bath at 55 ℃ for 3h, adding 1.5g of glycerol, continuing to stir for 1h, adding 0.0258g of dihydromyricetin, and continuing to stir for 10min to obtain a chitosan-dihydromyricetin film-forming solution II.
(2) And (3) pouring 20mL of the film forming solution I obtained in the step (1) into a film forming container (phi is 9cm), drying at 55 ℃ for 4h, then pouring 20mL of the film forming solution II, continuing to dry for 5h, and removing the film.
Composite membrane performance characterization and activity determination
1. The tensile strength and the breaking elongation of the degradable double-layer polysaccharide active membrane prepared by the method of the invention are measured, and the results are shown in table 1.
2. The antibacterial effect of the degradable double-layer polysaccharide active membrane prepared by the method on staphylococcus aureus is measured by a broth method, and the result is shown in table 2.
Test example 1
Determination of the tensile Properties of plastics according to GB/T1040.1-2018 [ < first part of the determination of tensile Properties of plastics ]: general rules the films prepared in examples 1, 2 and 3 and comparative examples 1 and 2 were tested for mechanical properties, and the results are shown in table 1. Tensile strength may reflect the maximum load bearing rate of the film under tensile conditions, and elongation at break may reflect the flexibility of the film.
TABLE 1 mechanical Property test results
| Index (I) | Example 1 | Example 2 | Example 3 | Comparative example 1 | Comparative example 2 |
| Tensile Strength (MPa) | 50.98 | 34.58 | 32.53 | 27.08 | 21.88 |
| Elongation at Break (%) | 24.31 | 18.85 | 19.73 | 15.77 | 14.01 |
From the results in table 1, it can be seen that the mechanical properties of the degradable bilayer polysaccharide active membranes prepared in examples 1-3 are better than those of the bilayer polysaccharide composite membranes prepared in comparative examples 1 and 2. The degradable double-layer polysaccharide active membrane prepared in example 1 has the best mechanical property, the tensile strength of the degradable double-layer polysaccharide active membrane is respectively higher than 88.16% and 133% of the double-layer polysaccharide composite membranes prepared in comparative examples 1 and 2, and the elongation at break of the degradable double-layer polysaccharide active membrane is respectively higher than 54.15% and 73.52% of the double-layer polysaccharide composite membranes prepared in comparative examples 1 and 2. In conclusion, the tensile strength and flexibility of the double-layer polysaccharide composite membrane can be optimized by adding the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound.
Test example 2
The inhibitory effect of the composite films prepared in examples 1, 2 and 3 and comparative examples 1 and 2 on the growth of staphylococcus aureus was measured to evaluate the antibacterial activity of the films.
(1) Staphylococcus aureus was inoculated into the nutrient broth and cultured overnight at 37 ℃ at 120rpm to obtain a strain in logarithmic growth phase. The concentration of the bacterial liquid was adjusted to 1X 10 with fresh sterile nutrient broth6CFU/mL to obtain inoculated bacterial liquid.
(2) Inoculating 2mL of the inoculated bacterial liquid obtained in the step (1) into 20mL of sterile nutrient broth, and adding 100-300 mg of the prepared sample membranes. Culturing at 37 ℃ and 120rpm for 16-18 h.
(3) And (3) taking 100 mu L of the culture in the step (2), coating the culture on nutrient agar culture medium, placing the culture at 37 ℃ for culturing for 12h, observing the growth condition of colonies and counting.
TABLE 2 results of the bacteriostatic activity test
As shown in the results in Table 2, the total number of colonies of the blank control was 2.8X 10 after 16-18 hours of culture8CFU/mL (8.63Log CFU/mL), compared with blank control, the concentration of the bacterial liquid after the treatment of the double-layer active composite membrane prepared in example 1, example 2 and example 3 is reduced by 2 orders of magnitude (10)6CFU/mL). The concentration of the treated bacterial liquid of the double-layer polysaccharide composite membranes prepared in comparative examples 1 and 2 was equivalent to that of the blank control (10)8CFU/mL), that is, the two-layer polysaccharide composite membranes prepared in comparative examples 1 and 2 do not have significant bacteriostatic activity. From the above data results, it was found that the addition of dihydromyricetin/hydroxypropyl- β -cyclodextrin inclusion compound provides the membrane with excellent antibacterial activity.
The foregoing shows and describes the general principles, principal features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention, which is intended to be covered by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (9)
1. A preparation method of a degradable double-layer polysaccharide active membrane comprises the following steps:
preparing a sodium alginate-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film forming solution I;
preparing a chitosan-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film forming solution II; and
and pouring the film forming solution I into a film forming container, drying at 50-60 ℃ for 3-4 h, then pouring the film forming solution II into the film forming container, continuously drying for 4-6 h, and finally uncovering the film to obtain the degradable double-layer polysaccharide active film.
2. The production method according to claim 1, wherein:
the sodium alginate-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film forming solution I is prepared by the following method:
adding sodium alginate into pure water, and stirring in a water bath at 50-60 ℃ for 2-4 h;
adding glycerol and stirring for 1-2 h; and
adding the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, and stirring for 10-30 min to obtain the product.
3. The production method according to claim 1, wherein:
the chitosan-dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound film forming solution II is prepared by the following method:
adding chitosan into an acetic acid aqueous solution with the volume fraction of 1%, and stirring in a water bath at 50-60 ℃ for 2-4 h;
adding glycerol and stirring for 1-2 hours; and
adding the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound, and stirring for 10-30 min to obtain the product.
4. The production method according to claim 1, wherein:
the mass ratio of the sodium alginate in the film forming solution I to the chitosan in the film forming solution II is 1: 1.
5. The production method according to claim 2 or 3, wherein:
the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound is prepared by the following method:
dissolving hydroxypropyl-beta-cyclodextrin in pure water, and continuously stirring at 45-50 ℃;
slowly adding a dihydromyricetin ethanol solution, and continuously stirring for reaction for 4-8 h; and
and (5) freeze-drying to obtain the product.
6. The production method according to claim 5, wherein:
the molar mass ratio of the dihydromyricetin to the hydroxypropyl-beta-cyclodextrin is 1: 1.
7. The production method according to claim 2, wherein:
the mass concentration of the sodium alginate and the chitosan is 1-2 wt%;
the mass concentration of the glycerol is 0.5-1 wt%;
the mass concentration of the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound is 0.05-0.3 wt%.
8. The production method according to claim 3, wherein:
the mass concentration of the sodium alginate and the chitosan is 1-2 wt%;
the mass concentration of the glycerol is 0.5-1 wt%;
the mass concentration of the dihydromyricetin/hydroxypropyl-beta-cyclodextrin inclusion compound is 0.05-0.3 wt%.
9. A degradable double-layer polysaccharide active membrane prepared according to any one of the preparation methods of claims 1-8.
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| CN104666293A (en) * | 2015-02-05 | 2015-06-03 | 江苏丰园生物技术有限公司 | Dihydromyricetin cyclodextrin inclusion compound and preparation method thereof |
| CN107840981A (en) * | 2017-11-08 | 2018-03-27 | 海南师范大学 | A kind of sodium alginate chitosan bilayer edible film, its preparation method and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104666293A (en) * | 2015-02-05 | 2015-06-03 | 江苏丰园生物技术有限公司 | Dihydromyricetin cyclodextrin inclusion compound and preparation method thereof |
| CN107840981A (en) * | 2017-11-08 | 2018-03-27 | 海南师范大学 | A kind of sodium alginate chitosan bilayer edible film, its preparation method and its application |
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| 离子交联壳聚糖/海藻酸钠可降解复合膜的研究;高美玲等;《中国海洋大学学报(自然科学版)》;20111015(第10期) * |
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