CN117187088A - Microbial preparation for surface water pollutant treatment, preparation method and application - Google Patents
Microbial preparation for surface water pollutant treatment, preparation method and application Download PDFInfo
- Publication number
- CN117187088A CN117187088A CN202311474253.8A CN202311474253A CN117187088A CN 117187088 A CN117187088 A CN 117187088A CN 202311474253 A CN202311474253 A CN 202311474253A CN 117187088 A CN117187088 A CN 117187088A
- Authority
- CN
- China
- Prior art keywords
- powder
- cyclodextrin
- cellulose acetate
- microbial preparation
- clamshell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 50
- 230000000813 microbial effect Effects 0.000 title claims abstract description 46
- 239000002352 surface water Substances 0.000 title claims abstract description 20
- 239000003344 environmental pollutant Substances 0.000 title claims abstract description 16
- 231100000719 pollutant Toxicity 0.000 title claims abstract description 16
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 36
- 230000001546 nitrifying effect Effects 0.000 claims abstract description 34
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 33
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 33
- 241000186361 Actinobacteria <class> Species 0.000 claims abstract description 32
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 241000186660 Lactobacillus Species 0.000 claims abstract description 25
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 25
- 239000004310 lactic acid Substances 0.000 claims abstract description 14
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 14
- 239000000843 powder Substances 0.000 claims description 80
- 229920002301 cellulose acetate Polymers 0.000 claims description 70
- 241000894006 Bacteria Species 0.000 claims description 59
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Natural products OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 58
- 229920000858 Cyclodextrin Polymers 0.000 claims description 53
- 238000002156 mixing Methods 0.000 claims description 50
- 239000002131 composite material Substances 0.000 claims description 45
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims description 42
- 238000000855 fermentation Methods 0.000 claims description 41
- 230000004151 fermentation Effects 0.000 claims description 41
- 238000005187 foaming Methods 0.000 claims description 41
- 244000005700 microbiome Species 0.000 claims description 38
- 235000021552 granulated sugar Nutrition 0.000 claims description 37
- 229920001661 Chitosan Polymers 0.000 claims description 35
- 239000006260 foam Substances 0.000 claims description 34
- -1 polypropylene Polymers 0.000 claims description 31
- 239000004088 foaming agent Substances 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 24
- 239000004743 Polypropylene Substances 0.000 claims description 17
- 229920001155 polypropylene Polymers 0.000 claims description 17
- 239000000155 melt Substances 0.000 claims description 16
- 238000006243 chemical reaction Methods 0.000 claims description 14
- 238000001125 extrusion Methods 0.000 claims description 14
- 239000010865 sewage Substances 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 12
- 239000001116 FEMA 4028 Substances 0.000 claims description 11
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 11
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 11
- 229960004853 betadex Drugs 0.000 claims description 11
- 238000000227 grinding Methods 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 239000007795 chemical reaction product Substances 0.000 claims description 5
- 239000002244 precipitate Substances 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000002844 melting Methods 0.000 claims description 2
- 230000008018 melting Effects 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 22
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 15
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 15
- 239000011574 phosphorus Substances 0.000 abstract description 15
- 239000000126 substance Substances 0.000 abstract description 13
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 8
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 4
- 229910019142 PO4 Inorganic materials 0.000 abstract description 4
- 239000001569 carbon dioxide Substances 0.000 abstract description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract description 4
- 239000010452 phosphate Substances 0.000 abstract description 4
- 238000001179 sorption measurement Methods 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 229930006000 Sucrose Natural products 0.000 abstract description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract description 2
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 abstract description 2
- 238000001556 precipitation Methods 0.000 abstract description 2
- 235000000346 sugar Nutrition 0.000 abstract description 2
- 235000013681 dietary sucrose Nutrition 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 14
- 239000004156 Azodicarbonamide Substances 0.000 description 7
- XOZUGNYVDXMRKW-AATRIKPKSA-N azodicarbonamide Chemical compound NC(=O)\N=N\C(N)=O XOZUGNYVDXMRKW-AATRIKPKSA-N 0.000 description 7
- 235000019399 azodicarbonamide Nutrition 0.000 description 7
- 229910000019 calcium carbonate Inorganic materials 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 241000195493 Cryptophyta Species 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 5
- 239000000920 calcium hydroxide Substances 0.000 description 5
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 5
- 229910021645 metal ion Inorganic materials 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- 238000010998 test method Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 230000001376 precipitating effect Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 229920002101 Chitin Polymers 0.000 description 2
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 2
- 239000000292 calcium oxide Substances 0.000 description 2
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 244000253911 Saccharomyces fragilis Species 0.000 description 1
- 235000018368 Saccharomyces fragilis Nutrition 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- DOLKKDJAWDNAMU-UHFFFAOYSA-N [4-[bis[4-(diethylamino)phenyl]methylidene]naphthalen-1-ylidene]-(4-methylphenyl)azanium;chloride Chemical compound [Cl-].C1=CC(N(CC)CC)=CC=C1C(C=1C=CC(=CC=1)N(CC)CC)=C(C=C1)C2=CC=CC=C2C1=[NH+]C1=CC=C(C)C=C1 DOLKKDJAWDNAMU-UHFFFAOYSA-N 0.000 description 1
- 125000000738 acetamido group Chemical group [H]C([H])([H])C(=O)N([H])[*] 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- FAHRAYOVAVLWAA-UHFFFAOYSA-L calcium;acetate;hydroxide Chemical compound [OH-].[Ca+2].CC([O-])=O FAHRAYOVAVLWAA-UHFFFAOYSA-L 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 229940031154 kluyveromyces marxianus Drugs 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 241001446247 uncultured actinomycete Species 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Environmental & Geological Engineering (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Virology (AREA)
- Water Supply & Treatment (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses a microbial preparation for treating surface water pollutants, a preparation method and application thereof, and belongs to the technical field of environmental treatment. The bacillus subtilis can decompose organic substances into micromolecular substances/carbon dioxide and water, and the actinomycetes can degrade the organic substances and COD; the nitrifying strain can degrade ammonia nitrogen, and can also convert phosphate into inorganic phosphorus, and then the inorganic phosphorus is removed by adsorption or precipitation; the lactobacillus can decompose sugar into lactic acid, the saccharose is decomposed into small molecular substances such as carbon dioxide and ethanol by the saccharomycetes, the rapid growth and propagation of the lactobacillus and the saccharomycetes are promoted, and the COD content, the ammonia nitrogen content and the phosphate content of the black and odorous water body are effectively degraded by the microbial preparation.
Description
Technical Field
The invention relates to the technical field of environmental treatment, in particular to a microbial preparation for treating surface water pollutants, a preparation method and application thereof.
Background
With the increasing of the living standard of people, the process of urban treatment is continuously accelerated, the sewage discharge amount of people per unit is continuously increased, clean water resources are increasingly tensioned, and surface water pollution is increasingly aggravated. In many cities of China, the surface landscape water bodies (river channels and lakes) are polluted to different degrees, so that various water quality indexes of the water bodies are seriously out of standard, and the indexes of nutrient salts such as nitrogen and phosphorus in part of the water bodies are obviously out of standard, thereby providing possibility for the large-scale growth and propagation of blue algae.
At present, the technology for treating the polluted surface water at home and abroad mainly comprises three methods of physics, chemistry and biology. The biological method can fundamentally restore the ecological function of the surface landscape, is a technology which is developed rapidly in recent years at home and abroad, uses cultivated plants or cultivated and inoculated microorganisms as microbial agents, uses the metabolic activity of the microorganisms to transfer, convert and degrade pollutants in water, improves the water quality of the water body, and constructs an aquatic ecological system with a complete nutrition level structure to fundamentally restore the health of the water body system. However, the existing microbial preparations still have low removal efficiency of contaminants.
Disclosure of Invention
In order to achieve the aim, the invention provides a microbial preparation for surface water pollutant treatment, which comprises compound microorganisms and a compound carrier solution, wherein the compound microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the compound carrier solution is an aqueous solution comprising clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin.
Preferably, the total number of viable microorganisms in the composite microorganism is greater than 5×10 7 cfu/ml, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 1-2:3-4:2-3:2-3:1-2.
Preferably, the mass ratio of the clamshell powder to the cellulose acetate foam powder to the brown granulated sugar to the chitosan to the cyclodextrin is 5-10:10-20:15-25:1-5, wherein the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 25-40wt%.
Preferably, the clamshell powder-cellulose acetate foam powder is prepared by the following steps:
mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture;
and (3) melting, extruding and foaming the mixture, polypropylene and a foaming agent to obtain the clamshell powder-cellulose acetate foaming powder.
Preferably, the mass ratio of the clamshell powder to the cellulose acetate is: (25-30): (10-15); the mass ratio of the mixture to the polypropylene to the foaming agent is (35-45): (5-15): 1-8; the temperature of the melt extrusion is 140-160 ℃, and the melt blending is carried out for 8-12min; foaming temperature is 180-210 deg.C, foaming is 5-20min.
Preferably, the cyclodextrin is citric acid modified cyclodextrin, and the citric acid modified cyclodextrin is prepared by the following steps: beta-cyclodextrin, citric acid and disodium hydrogen phosphate are mixed for reaction, and the precipitate is washed to obtain a reaction product.
Preferably, the molar mass ratio of beta-cyclodextrin, citric acid and disodium hydrogen phosphate is 1: (2-4): (1-1.5); the temperature of the mixing reaction is 70-80 ℃ and the reaction lasts for 60-120 min.
A method for preparing said microbial preparation comprising the steps of:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
and uniformly mixing the fermentation liquor and the composite carrier to obtain the microbial preparation.
Preferably, the mass ratio of the fermentation liquor to the composite carrier is 1: (1.5-3).
The microbial preparation is applied to sewage treatment.
The invention has the beneficial effects that:
(1) The actinomycete strain can effectively degrade organic matters in the water body, so that COD in the water body is degraded; the bacillus subtilis can decompose organic substances in the water body into micromolecular substances/carbon dioxide and water, and the micromolecular substances are further decomposed; the nitrifying strain can degrade ammonia nitrogen in the water body, and can also convert phosphate into inorganic phosphorus through nitrifying reaction, and then remove the inorganic phosphorus through cyclodextrin adsorption or through metal ion precipitation; lactic acid bacteria can decompose sugar into lactic acid and other small molecular substances; saccharomycete is used as a fungus to decompose sucrose into carbon dioxide, ethanol and other small molecular substances, and meanwhile, the rapid growth and propagation of lactic acid bacteria and saccharomycete are promoted. The lactobacillus and the saccharomycetes have interaction promotion effect in the fermentation process, so that the stability of the number of living bacteria can be maintained by utilizing the interaction relation between the lactobacillus and the saccharomycetes while a large number of strains are generated. Therefore, the COD content, the ammonia nitrogen content and the phosphate content of the black and odorous water body can be effectively degraded, and the odor of the black and odorous water body can be eliminated. Experiments prove that the removal rates of the algae, total nitrogen, total phosphorus and COD respectively reach 95-99%, 90-95% and 90-95%.
The clamshell powder-cellulose acetate foaming powder prepared by microporous foaming is formed into a netlike fiber framework by cellulose acetate, and the clamshell powder is dispersed and fixed on the netlike fiber framework to form the microThe pore diameter of the pores is between 10 mu m and 100 mu m, and the pore density is 10 5 -10 9 Thus having abundant fiber network and porous structure; the main components of the clamshell powder are calcium oxide and chitin, and after the clamshell powder-cellulose acetate foam powder is dissolved in water, the calcium oxide is hydrolyzed to obtain calcium hydroxide/calcium carbonate, so that a calcium hydroxide/calcium carbonate-cellulose acetate porous structure is obtained, and microorganisms can be effectively adsorbed between cellulose acetate-calcium hydroxide/calcium carbonate. The brown granulated sugar and the chitosan are used as carbon sources, so that the growth and the propagation of microorganisms can be promoted, and a large amount of generated thalli are utilized to degrade organic matters in sewage to generate harmless substances, so that the sewage reaches the discharge standard, and the harm of sewage discharge to the environment is reduced. The chitosan molecular chain is rich in hydroxyl and amino, can be complexed with calcium hydroxide and calcium carbonate, has strong adsorption capacity, can adsorb microorganisms, and ensures that the microorganisms are more uniformly dispersed between cellulose acetate and calcium hydroxide/calcium carbonate, and have lasting activity. Chitin is acetamido (-NHCOCH) 2 ) It contains-NH 2 The radical, -OH, can complex with cyclodextrin, has strong adsorption capacity, can adsorb microorganisms, and ensures that the microorganisms are uniformly dispersed and have lasting activity. The cyclodextrin has hydrophilic periphery and hydrophobic inner cavity, the main body structure is like a hollow in the middle and a barrel with two non-closed ends, the hydrophobic cavity can envelop organic micromolecules, metal ions and inorganic phosphorus which absorb microbial decomposition, and the metal ions can also precipitate with the inorganic phosphorus, so that the metal ions and the inorganic phosphorus are removed simultaneously. And the characteristic of the cyclodextrin that the cyclodextrin is hydrophilic can improve the wettability of brown granulated sugar, and is beneficial to dispersing the brown granulated sugar.
(2) And the citric acid modified cyclodextrin is adopted, and citrate ions can be chelated with calcium ions of the clamshell powder, so that the combination of the citric acid modified cyclodextrin and calcium hydroxide/calcium carbonate is enhanced, and the citrate ions can form a complex with metal cations in sewage, so that the metal ions in the sewage are better enveloped and absorbed.
Detailed Description
Using parameters of specific chemicals, sources:
the lactobacillus is Lactobacillus acidophilus with the model PG-LAS107 manufactured by Shandong Jiacheng biotechnology Co., ltd; the yeast is Kluyveromyces marxianus FJ972218.1; actinomycetes are fine Huang Fangxian bacteria produced by Yiyuan Kangyuan biotechnology Co., ltd; the bacillus subtilis is produced by Jinan Jin Yuyuan biotechnology Co., ltd, and the model is 2018009; the nitrifying bacteria are commercial RY-JW003 strains manufactured by Guangzhou natural beneficial organisms environmental protection technology Co., ltd; the clamshell powder is sea shell powder produced by Shaanxi green Cheng biological product manufacturing company, and the model is SXLSYHGKTQW; cellulose acetate is produced by Nantong Runfeng petrochemical industry Co., ltd, and the CAS number is 9004-35-7; polypropylene is produced by Shanghai bridge micro chemical technology Co., ltd, and the product number is GM1600E; the azodicarbonamide foaming agent is AC foaming agent produced by Jinan night blue biotechnology Co., ltd; the brown granulated sugar is brown granulated sugar (food grade, raw material is sugarcane) produced by Shandong Jade chemical Co., ltd; chitosan is produced by Shaanxi Chenming biotechnology Co., ltd, and CAS number is 9012-76-4; beta-cyclodextrin is produced by Henan Huachi Biotechnology Co., ltd, and the CAS number is 68168-23-0;
citric acid is produced by Shandong Jinao chemical industry Co., ltd, and the CAS number is 77-92-9; disodium hydrogen phosphate is produced by Hefei Shengrun biological products Co., ltd, and the CAS number is 7558-79-4.
Example 1
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin, and the ratio of the viable count of the saccharomycetes, the lactic acid bacteria, the actinomycetes, the bacillus subtilis and the nitrifying bacteria is 1:3:2:2:1, the total number of microorganism bacteria is 7.2X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 5:10:15:1, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 25wt%. Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clam shell powder and cellulose acetate to obtain a mixture, wherein the clam shell powderAnd cellulose acetate in the following mass ratio: 25:10; the method comprises the steps of performing melt extrusion on a mixture, polypropylene and a foaming agent, and foaming to obtain clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 35:5:1, a step of; the temperature of the melt extrusion is 140 ℃, and the melt blending is carried out for 8min; foaming temperature is 180 ℃, and foaming is carried out for 5min; the foaming agent is azodicarbonamide.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:1.5.
example 2
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin, and the ratio of the viable count of the saccharomycetes, the lactic acid bacteria, the actinomycetes, the bacillus subtilis and the nitrifying bacteria is 2:4:3:3:2, the total number of microorganism bacteria is 7.5X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 10:20:25:5, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 40wt%.
Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture, wherein the mass ratio of the clamshell powder to the cellulose acetate is 30:15; the method comprises the steps of performing melt extrusion on a mixture, polypropylene and a foaming agent, and foaming to obtain clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 45:15:8, 8; the temperature of the melt extrusion is 160 ℃, and the melt blending is carried out for 12min; foaming temperature is 210 ℃, foaming is carried out for 20min; the foaming agent is azodicarbonamide.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:3.
example 3
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin. Wherein, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 2:3:2:3:2, the total number of microorganism bacteria is 7.1X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 8:15:20:3, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 30wt%.
Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture, wherein the mass ratio of the clamshell powder to the cellulose acetate is as follows: 28:12; and (3) carrying out melt extrusion and foaming on the mixture, the polypropylene and the foaming agent to obtain the clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 40:10:5, a step of; the temperature of the melt extrusion is 150 ℃, and the melt blending is carried out for 10min; foaming temperature is 200 ℃, foaming is carried out for 10min; the foaming agent is azodicarbonamide.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
example 4
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin. Wherein, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 2:3:2:3:2, the total number of microorganism bacteria is 7.1X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 8:15:20:3, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 30wt%.
Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture, wherein the mass ratio of the clamshell powder to the cellulose acetate is as follows: 28:12; and (3) carrying out melt extrusion and foaming on the mixture, the polypropylene and the foaming agent to obtain the clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 40:10:5, a step of; the temperature of the melt extrusion is 150 ℃, and the melt blending is carried out for 10min; foaming temperature is 200 ℃, foaming is carried out for 10min; the foaming agent is azodicarbonamide.
The cyclodextrin is citric acid modified cyclodextrin, and the citric acid modified cyclodextrin is prepared by the following steps: mixing beta-cyclodextrin, citric acid and disodium hydrogen phosphate for reaction, precipitating and washing to obtain a reaction product, wherein the molar mass ratio of the beta-cyclodextrin to the citric acid to the disodium hydrogen phosphate is 1:2:1, a step of; the temperature of the mixing reaction was 70℃for 60min.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
example 5
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin. Wherein, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 2:3:2:3:2, the total number of microorganism bacteria is 7.1X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 8:15:20:3, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 30wt%.
Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture, wherein the mass ratio of the clamshell powder to the cellulose acetate is as follows: 28:12; and (3) carrying out melt extrusion and foaming on the mixture, the polypropylene and the foaming agent to obtain the clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 40:10:5, a step of; the temperature of the melt extrusion is 150 ℃, and the melt blending is carried out for 10min; foaming temperature is 200 ℃, foaming is carried out for 10min; the foaming agent is azodicarbonamide.
The cyclodextrin is citric acid modified cyclodextrin, and the citric acid modified cyclodextrin is prepared by the following steps: mixing beta-cyclodextrin, citric acid and disodium hydrogen phosphate for reaction, precipitating and washing to obtain a reaction product, wherein the molar mass ratio of the beta-cyclodextrin to the citric acid to the disodium hydrogen phosphate is 1:4:1.5; the temperature of the mixed reaction is 780 ℃ and the reaction is 120min.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
example 6
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin. Wherein, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 2:3:2:3:2, the total number of microorganism bacteria is 7.1X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 8:15:20:3, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 30wt%.
Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture, wherein the mass ratio of the clamshell powder to the cellulose acetate is as follows: 28:12; and (3) carrying out melt extrusion and foaming on the mixture, the polypropylene and the foaming agent to obtain the clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 40:10:5, a step of; the temperature of the melt extrusion is 150 ℃, and the melt blending is carried out for 10min; foaming temperature is 200 ℃, foaming is carried out for 10min; the foaming agent is azodicarbonamide.
The cyclodextrin is citric acid modified cyclodextrin, and the citric acid modified cyclodextrin is prepared by the following steps: mixing beta-cyclodextrin, citric acid and disodium hydrogen phosphate for reaction, precipitating and washing to obtain a reaction product, wherein the molar mass ratio of the beta-cyclodextrin to the citric acid to the disodium hydrogen phosphate is 1:3:1.2; the temperature of the mixed reaction was 75℃for 80min.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
comparative example 1
The comparative example provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a carrier, wherein the microorganisms comprise saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria, the carrier is calcium carbonate powder, and the ratio of the number of viable bacteria of the saccharomycetes, the lactobacillus, the actinomycetes, the bacillus subtilis and the nitrifying bacteria is 1:3:2:2:1.
the preparation method comprises the following steps: respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
comparative example 2
The comparative example provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a carrier, wherein the microorganisms comprise saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria, the carrier is calcium carbonate powder, and the ratio of the number of viable bacteria of the saccharomycetes, the lactobacillus, the actinomycetes, the bacillus subtilis and the nitrifying bacteria is 1:1.5:1:1:0.5.
the preparation method comprises the following steps: respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
application and test: the prepared microbial preparation is added into polluted surface water (the COD concentration in the polluted surface water in the test is 102mg/L, the total nitrogen concentration is 6.4mg/L, the total phosphorus concentration is 3.6mg/L and the algae concentration is 32.1 mg/L), and the addition amount is 1.5% of the volume of the polluted surface water; the treatment time was 7 days. The COD in the sewage before and after the treatment of the test method is measured by adopting a potassium dichromate method, the total nitrogen concentration in the sewage before and after the treatment of the test method is measured by adopting a potassium persulfate oxidation-ultraviolet spectrophotometry, and the total phosphorus in the sewage before and after the treatment of the test method is measured by adopting an ammonium molybdate spectrophotometry; the algae in the sewage before and after the treatment of the test method are measured by a microscope. The test data are shown in Table 1.
TABLE 1
As can be seen from Table 1, the removal rates of algae, total nitrogen, total phosphorus and COD in examples 1-6 reach 95-99%, 94-99%, 89-97%, 92-95%, respectively, the content of COD, total nitrogen and total phosphorus in the treated polluted river water reaches the quality standard of surface water environment (GB 3838-2002) class III water quality standard, and the content indexes of COD, ammonia nitrogen and total phosphorus in the treated sewage water reach the first grade B standard of pollutant emission standard of urban sewage treatment plant (GB 18918-2002). And the removal rates of the algae, the total nitrogen, the total phosphorus and the COD in the comparative examples 1 and 2 reach 79 to 81 percent, 72 to 78 percent, 61 to 69 percent and 77 to 80 percent respectively. Thus, examples 1-6 are significantly better than comparative examples 1 and 2.
Claims (10)
1. The microbial preparation for surface water pollutant treatment is characterized in that: the composite microbial preparation comprises composite microorganisms and a composite carrier solution, wherein the composite microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the composite carrier solution is an aqueous solution comprising clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin.
2. A microbial preparation according to claim 1, characterized in that: the total number of viable microorganisms in the composite microorganism is more than 5 multiplied by 10 7 cfu/ml, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 1-2:3-4:2-3:2-3:1-2.
3. A microbial preparation according to claim 1, characterized in that: the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 5-10:10-20:15-25:1-5, wherein the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 25-40wt%.
4. A microbial preparation according to claim 1, characterized in that: the clamshell powder-cellulose acetate foam powder is prepared by the following steps:
mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture;
and (3) melting, extruding and foaming the mixture, polypropylene and a foaming agent to obtain the clamshell powder-cellulose acetate foaming powder.
5. The microbial preparation according to claim 4, wherein: the mass ratio of the clamshell powder to the cellulose acetate is as follows: (25-30): (10-15); the mass ratio of the mixture to the polypropylene to the foaming agent is (35-45): (5-15): 1-8; the temperature of the melt extrusion is 140-160 ℃, and the melt blending is carried out for 8-12min; foaming temperature is 180-210 deg.C, foaming is 5-20min.
6. A microbial preparation according to claim 1, characterized in that: the cyclodextrin is citric acid modified cyclodextrin, and the citric acid modified cyclodextrin is prepared by the following steps: beta-cyclodextrin, citric acid and disodium hydrogen phosphate are mixed for reaction, and the precipitate is washed to obtain a reaction product.
7. The microbial preparation according to claim 6, wherein: the molar mass ratio of the beta-cyclodextrin to the citric acid to the disodium hydrogen phosphate is 1: (2-4): (1-1.5); the temperature of the mixing reaction is 70-80 ℃ and the reaction lasts for 60-120 min.
8. A process for preparing a microbial preparation according to any one of claims 1 to 7, characterized by comprising the steps of:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
and uniformly mixing the fermentation liquor and the composite carrier to obtain the microbial preparation.
9. The method according to claim 8, wherein the mass ratio of the fermentation broth to the composite carrier is 1: (1.5-3).
10. Use of the microbial preparation of any one of claims 1-7 in sewage treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311474253.8A CN117187088A (en) | 2023-11-08 | 2023-11-08 | Microbial preparation for surface water pollutant treatment, preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311474253.8A CN117187088A (en) | 2023-11-08 | 2023-11-08 | Microbial preparation for surface water pollutant treatment, preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117187088A true CN117187088A (en) | 2023-12-08 |
Family
ID=88992838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311474253.8A Pending CN117187088A (en) | 2023-11-08 | 2023-11-08 | Microbial preparation for surface water pollutant treatment, preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117187088A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101082029A (en) * | 2006-06-01 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of water purification bacterium and method for restoring aquaculture environment |
| CN106148246A (en) * | 2016-08-30 | 2016-11-23 | 蓝德环保科技集团股份有限公司 | Purify compounding microbial inoculum of black and odorous water and preparation method thereof |
| CN106145375A (en) * | 2015-04-17 | 2016-11-23 | 南京莎菲特生物科技有限公司 | A kind of preparation and application method for removing sewage and the compositions educating river course again |
| CN106673165A (en) * | 2015-11-06 | 2017-05-17 | 周坤友 | High-molecular chitosan composite flocculant |
| CN107500419A (en) * | 2017-09-28 | 2017-12-22 | 合肥市东方美捷分子材料技术有限公司 | One kind administers black and odorous water sustained release QQ balls and its processing method and application |
| CN111115843A (en) * | 2019-12-25 | 2020-05-08 | 安徽麓瑞科技有限公司 | Combined water quality improver for freshwater fish culture pond |
| CN114746541A (en) * | 2019-12-16 | 2022-07-12 | 4D制药研究有限公司 | Providing bacterial biomass with improved storage stability |
-
2023
- 2023-11-08 CN CN202311474253.8A patent/CN117187088A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101082029A (en) * | 2006-06-01 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of water purification bacterium and method for restoring aquaculture environment |
| CN106145375A (en) * | 2015-04-17 | 2016-11-23 | 南京莎菲特生物科技有限公司 | A kind of preparation and application method for removing sewage and the compositions educating river course again |
| CN106673165A (en) * | 2015-11-06 | 2017-05-17 | 周坤友 | High-molecular chitosan composite flocculant |
| CN106148246A (en) * | 2016-08-30 | 2016-11-23 | 蓝德环保科技集团股份有限公司 | Purify compounding microbial inoculum of black and odorous water and preparation method thereof |
| CN107500419A (en) * | 2017-09-28 | 2017-12-22 | 合肥市东方美捷分子材料技术有限公司 | One kind administers black and odorous water sustained release QQ balls and its processing method and application |
| CN114746541A (en) * | 2019-12-16 | 2022-07-12 | 4D制药研究有限公司 | Providing bacterial biomass with improved storage stability |
| CN111115843A (en) * | 2019-12-25 | 2020-05-08 | 安徽麓瑞科技有限公司 | Combined water quality improver for freshwater fish culture pond |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109536173B (en) | Composite material for simultaneously repairing heavy metal and glyphosate and preparation method thereof | |
| CN116621320B (en) | Biological composite carbon source prepared from blue algae and preparation process thereof | |
| CN110255722A (en) | A kind of dyeing waste water treatment based on immobilized microorganisms agent and preparation method thereof | |
| CN111206027B (en) | Magnetic biochar suitable for loading bacillus and preparation method and application thereof | |
| CN110317801A (en) | Bacterium plural gel bead and its preparation method and application is quenched in a kind of immobilization quorum sensing | |
| CN115491324A (en) | Microbial agent for high-speed humification of organic waste and preparation method thereof | |
| CN114908002A (en) | Biological nano-selenium reinforced composite strain and application thereof | |
| CN106430630A (en) | Antibiotic waste water treatment agent | |
| CN115786191B (en) | Citrobacter freundii and application thereof in pesticide production wastewater treatment | |
| CN115710564B (en) | Microbial adsorbent and preparation method and application thereof | |
| CN109897869B (en) | Biological activated carbon cultured by high-sugar wastewater and using ficus microcarpa leaves as carbon source carrier | |
| CN117187088A (en) | Microbial preparation for surface water pollutant treatment, preparation method and application | |
| CN114517195B (en) | Microbial composition and preparation method thereof | |
| CN114031189B (en) | Method for treating sewage with low carbon-nitrogen ratio | |
| CN114108319B (en) | Preparation method and application of gel fiber embedded with Shewanella | |
| CN109896703B (en) | Light-enzyme composite catalytic function microorganism water purifying agent for culturing anaerobic sewage | |
| CN113698039A (en) | Harmless treatment method and system for urine and intelligent toilet based on harmless treatment method and system | |
| CN111498987A (en) | Domestic sewage in-situ purification immobilized carrier and microbial inoculum loading method | |
| CN112479390A (en) | Compound for organic sewage treatment and preparation process thereof | |
| CN111747786A (en) | Compound fermentation soil remediation agent and preparation method thereof | |
| CN114958686B (en) | Quinone-based modified biochar-based microbial agent and preparation and application thereof | |
| CN117904011B (en) | Microbial agent for treating high-salt refractory industrial wastewater and preparation method and application thereof | |
| CN112980730B (en) | Mixed microbial inoculum capable of degrading mixed wastewater of n-butyl alcohol and propylene glycol monobutyl ether and application thereof | |
| CN115558684B (en) | Biological preparation method of amide compound and acrylamide prepared by biological method | |
| CN118515362B (en) | Composite carbon source for denitrification treatment of photovoltaic wastewater and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20231208 |