CN117187088A - Microbial preparation for surface water pollutant treatment, preparation method and application - Google Patents

Microbial preparation for surface water pollutant treatment, preparation method and application Download PDF

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CN117187088A
CN117187088A CN202311474253.8A CN202311474253A CN117187088A CN 117187088 A CN117187088 A CN 117187088A CN 202311474253 A CN202311474253 A CN 202311474253A CN 117187088 A CN117187088 A CN 117187088A
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powder
cyclodextrin
cellulose acetate
microbial preparation
clamshell
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阎墨
宁超群
李洪亮
孔凡庸
石月梅
马啸
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Jilin Zhongke Zhaoxiang Ecological Technology Group Co ltd
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Abstract

The invention discloses a microbial preparation for treating surface water pollutants, a preparation method and application thereof, and belongs to the technical field of environmental treatment. The bacillus subtilis can decompose organic substances into micromolecular substances/carbon dioxide and water, and the actinomycetes can degrade the organic substances and COD; the nitrifying strain can degrade ammonia nitrogen, and can also convert phosphate into inorganic phosphorus, and then the inorganic phosphorus is removed by adsorption or precipitation; the lactobacillus can decompose sugar into lactic acid, the saccharose is decomposed into small molecular substances such as carbon dioxide and ethanol by the saccharomycetes, the rapid growth and propagation of the lactobacillus and the saccharomycetes are promoted, and the COD content, the ammonia nitrogen content and the phosphate content of the black and odorous water body are effectively degraded by the microbial preparation.

Description

Microbial preparation for surface water pollutant treatment, preparation method and application
Technical Field
The invention relates to the technical field of environmental treatment, in particular to a microbial preparation for treating surface water pollutants, a preparation method and application thereof.
Background
With the increasing of the living standard of people, the process of urban treatment is continuously accelerated, the sewage discharge amount of people per unit is continuously increased, clean water resources are increasingly tensioned, and surface water pollution is increasingly aggravated. In many cities of China, the surface landscape water bodies (river channels and lakes) are polluted to different degrees, so that various water quality indexes of the water bodies are seriously out of standard, and the indexes of nutrient salts such as nitrogen and phosphorus in part of the water bodies are obviously out of standard, thereby providing possibility for the large-scale growth and propagation of blue algae.
At present, the technology for treating the polluted surface water at home and abroad mainly comprises three methods of physics, chemistry and biology. The biological method can fundamentally restore the ecological function of the surface landscape, is a technology which is developed rapidly in recent years at home and abroad, uses cultivated plants or cultivated and inoculated microorganisms as microbial agents, uses the metabolic activity of the microorganisms to transfer, convert and degrade pollutants in water, improves the water quality of the water body, and constructs an aquatic ecological system with a complete nutrition level structure to fundamentally restore the health of the water body system. However, the existing microbial preparations still have low removal efficiency of contaminants.
Disclosure of Invention
In order to achieve the aim, the invention provides a microbial preparation for surface water pollutant treatment, which comprises compound microorganisms and a compound carrier solution, wherein the compound microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the compound carrier solution is an aqueous solution comprising clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin.
Preferably, the total number of viable microorganisms in the composite microorganism is greater than 5×10 7 cfu/ml, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 1-2:3-4:2-3:2-3:1-2.
Preferably, the mass ratio of the clamshell powder to the cellulose acetate foam powder to the brown granulated sugar to the chitosan to the cyclodextrin is 5-10:10-20:15-25:1-5, wherein the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 25-40wt%.
Preferably, the clamshell powder-cellulose acetate foam powder is prepared by the following steps:
mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture;
and (3) melting, extruding and foaming the mixture, polypropylene and a foaming agent to obtain the clamshell powder-cellulose acetate foaming powder.
Preferably, the mass ratio of the clamshell powder to the cellulose acetate is: (25-30): (10-15); the mass ratio of the mixture to the polypropylene to the foaming agent is (35-45): (5-15): 1-8; the temperature of the melt extrusion is 140-160 ℃, and the melt blending is carried out for 8-12min; foaming temperature is 180-210 deg.C, foaming is 5-20min.
Preferably, the cyclodextrin is citric acid modified cyclodextrin, and the citric acid modified cyclodextrin is prepared by the following steps: beta-cyclodextrin, citric acid and disodium hydrogen phosphate are mixed for reaction, and the precipitate is washed to obtain a reaction product.
Preferably, the molar mass ratio of beta-cyclodextrin, citric acid and disodium hydrogen phosphate is 1: (2-4): (1-1.5); the temperature of the mixing reaction is 70-80 ℃ and the reaction lasts for 60-120 min.
A method for preparing said microbial preparation comprising the steps of:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
and uniformly mixing the fermentation liquor and the composite carrier to obtain the microbial preparation.
Preferably, the mass ratio of the fermentation liquor to the composite carrier is 1: (1.5-3).
The microbial preparation is applied to sewage treatment.
The invention has the beneficial effects that:
(1) The actinomycete strain can effectively degrade organic matters in the water body, so that COD in the water body is degraded; the bacillus subtilis can decompose organic substances in the water body into micromolecular substances/carbon dioxide and water, and the micromolecular substances are further decomposed; the nitrifying strain can degrade ammonia nitrogen in the water body, and can also convert phosphate into inorganic phosphorus through nitrifying reaction, and then remove the inorganic phosphorus through cyclodextrin adsorption or through metal ion precipitation; lactic acid bacteria can decompose sugar into lactic acid and other small molecular substances; saccharomycete is used as a fungus to decompose sucrose into carbon dioxide, ethanol and other small molecular substances, and meanwhile, the rapid growth and propagation of lactic acid bacteria and saccharomycete are promoted. The lactobacillus and the saccharomycetes have interaction promotion effect in the fermentation process, so that the stability of the number of living bacteria can be maintained by utilizing the interaction relation between the lactobacillus and the saccharomycetes while a large number of strains are generated. Therefore, the COD content, the ammonia nitrogen content and the phosphate content of the black and odorous water body can be effectively degraded, and the odor of the black and odorous water body can be eliminated. Experiments prove that the removal rates of the algae, total nitrogen, total phosphorus and COD respectively reach 95-99%, 90-95% and 90-95%.
The clamshell powder-cellulose acetate foaming powder prepared by microporous foaming is formed into a netlike fiber framework by cellulose acetate, and the clamshell powder is dispersed and fixed on the netlike fiber framework to form the microThe pore diameter of the pores is between 10 mu m and 100 mu m, and the pore density is 10 5 -10 9 Thus having abundant fiber network and porous structure; the main components of the clamshell powder are calcium oxide and chitin, and after the clamshell powder-cellulose acetate foam powder is dissolved in water, the calcium oxide is hydrolyzed to obtain calcium hydroxide/calcium carbonate, so that a calcium hydroxide/calcium carbonate-cellulose acetate porous structure is obtained, and microorganisms can be effectively adsorbed between cellulose acetate-calcium hydroxide/calcium carbonate. The brown granulated sugar and the chitosan are used as carbon sources, so that the growth and the propagation of microorganisms can be promoted, and a large amount of generated thalli are utilized to degrade organic matters in sewage to generate harmless substances, so that the sewage reaches the discharge standard, and the harm of sewage discharge to the environment is reduced. The chitosan molecular chain is rich in hydroxyl and amino, can be complexed with calcium hydroxide and calcium carbonate, has strong adsorption capacity, can adsorb microorganisms, and ensures that the microorganisms are more uniformly dispersed between cellulose acetate and calcium hydroxide/calcium carbonate, and have lasting activity. Chitin is acetamido (-NHCOCH) 2 ) It contains-NH 2 The radical, -OH, can complex with cyclodextrin, has strong adsorption capacity, can adsorb microorganisms, and ensures that the microorganisms are uniformly dispersed and have lasting activity. The cyclodextrin has hydrophilic periphery and hydrophobic inner cavity, the main body structure is like a hollow in the middle and a barrel with two non-closed ends, the hydrophobic cavity can envelop organic micromolecules, metal ions and inorganic phosphorus which absorb microbial decomposition, and the metal ions can also precipitate with the inorganic phosphorus, so that the metal ions and the inorganic phosphorus are removed simultaneously. And the characteristic of the cyclodextrin that the cyclodextrin is hydrophilic can improve the wettability of brown granulated sugar, and is beneficial to dispersing the brown granulated sugar.
(2) And the citric acid modified cyclodextrin is adopted, and citrate ions can be chelated with calcium ions of the clamshell powder, so that the combination of the citric acid modified cyclodextrin and calcium hydroxide/calcium carbonate is enhanced, and the citrate ions can form a complex with metal cations in sewage, so that the metal ions in the sewage are better enveloped and absorbed.
Detailed Description
Using parameters of specific chemicals, sources:
the lactobacillus is Lactobacillus acidophilus with the model PG-LAS107 manufactured by Shandong Jiacheng biotechnology Co., ltd; the yeast is Kluyveromyces marxianus FJ972218.1; actinomycetes are fine Huang Fangxian bacteria produced by Yiyuan Kangyuan biotechnology Co., ltd; the bacillus subtilis is produced by Jinan Jin Yuyuan biotechnology Co., ltd, and the model is 2018009; the nitrifying bacteria are commercial RY-JW003 strains manufactured by Guangzhou natural beneficial organisms environmental protection technology Co., ltd; the clamshell powder is sea shell powder produced by Shaanxi green Cheng biological product manufacturing company, and the model is SXLSYHGKTQW; cellulose acetate is produced by Nantong Runfeng petrochemical industry Co., ltd, and the CAS number is 9004-35-7; polypropylene is produced by Shanghai bridge micro chemical technology Co., ltd, and the product number is GM1600E; the azodicarbonamide foaming agent is AC foaming agent produced by Jinan night blue biotechnology Co., ltd; the brown granulated sugar is brown granulated sugar (food grade, raw material is sugarcane) produced by Shandong Jade chemical Co., ltd; chitosan is produced by Shaanxi Chenming biotechnology Co., ltd, and CAS number is 9012-76-4; beta-cyclodextrin is produced by Henan Huachi Biotechnology Co., ltd, and the CAS number is 68168-23-0;
citric acid is produced by Shandong Jinao chemical industry Co., ltd, and the CAS number is 77-92-9; disodium hydrogen phosphate is produced by Hefei Shengrun biological products Co., ltd, and the CAS number is 7558-79-4.
Example 1
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin, and the ratio of the viable count of the saccharomycetes, the lactic acid bacteria, the actinomycetes, the bacillus subtilis and the nitrifying bacteria is 1:3:2:2:1, the total number of microorganism bacteria is 7.2X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 5:10:15:1, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 25wt%. Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clam shell powder and cellulose acetate to obtain a mixture, wherein the clam shell powderAnd cellulose acetate in the following mass ratio: 25:10; the method comprises the steps of performing melt extrusion on a mixture, polypropylene and a foaming agent, and foaming to obtain clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 35:5:1, a step of; the temperature of the melt extrusion is 140 ℃, and the melt blending is carried out for 8min; foaming temperature is 180 ℃, and foaming is carried out for 5min; the foaming agent is azodicarbonamide.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:1.5.
example 2
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin, and the ratio of the viable count of the saccharomycetes, the lactic acid bacteria, the actinomycetes, the bacillus subtilis and the nitrifying bacteria is 2:4:3:3:2, the total number of microorganism bacteria is 7.5X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 10:20:25:5, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 40wt%.
Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture, wherein the mass ratio of the clamshell powder to the cellulose acetate is 30:15; the method comprises the steps of performing melt extrusion on a mixture, polypropylene and a foaming agent, and foaming to obtain clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 45:15:8, 8; the temperature of the melt extrusion is 160 ℃, and the melt blending is carried out for 12min; foaming temperature is 210 ℃, foaming is carried out for 20min; the foaming agent is azodicarbonamide.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:3.
example 3
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin. Wherein, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 2:3:2:3:2, the total number of microorganism bacteria is 7.1X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 8:15:20:3, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 30wt%.
Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture, wherein the mass ratio of the clamshell powder to the cellulose acetate is as follows: 28:12; and (3) carrying out melt extrusion and foaming on the mixture, the polypropylene and the foaming agent to obtain the clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 40:10:5, a step of; the temperature of the melt extrusion is 150 ℃, and the melt blending is carried out for 10min; foaming temperature is 200 ℃, foaming is carried out for 10min; the foaming agent is azodicarbonamide.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
example 4
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin. Wherein, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 2:3:2:3:2, the total number of microorganism bacteria is 7.1X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 8:15:20:3, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 30wt%.
Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture, wherein the mass ratio of the clamshell powder to the cellulose acetate is as follows: 28:12; and (3) carrying out melt extrusion and foaming on the mixture, the polypropylene and the foaming agent to obtain the clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 40:10:5, a step of; the temperature of the melt extrusion is 150 ℃, and the melt blending is carried out for 10min; foaming temperature is 200 ℃, foaming is carried out for 10min; the foaming agent is azodicarbonamide.
The cyclodextrin is citric acid modified cyclodextrin, and the citric acid modified cyclodextrin is prepared by the following steps: mixing beta-cyclodextrin, citric acid and disodium hydrogen phosphate for reaction, precipitating and washing to obtain a reaction product, wherein the molar mass ratio of the beta-cyclodextrin to the citric acid to the disodium hydrogen phosphate is 1:2:1, a step of; the temperature of the mixing reaction was 70℃for 60min.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
example 5
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin. Wherein, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 2:3:2:3:2, the total number of microorganism bacteria is 7.1X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 8:15:20:3, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 30wt%.
Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture, wherein the mass ratio of the clamshell powder to the cellulose acetate is as follows: 28:12; and (3) carrying out melt extrusion and foaming on the mixture, the polypropylene and the foaming agent to obtain the clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 40:10:5, a step of; the temperature of the melt extrusion is 150 ℃, and the melt blending is carried out for 10min; foaming temperature is 200 ℃, foaming is carried out for 10min; the foaming agent is azodicarbonamide.
The cyclodextrin is citric acid modified cyclodextrin, and the citric acid modified cyclodextrin is prepared by the following steps: mixing beta-cyclodextrin, citric acid and disodium hydrogen phosphate for reaction, precipitating and washing to obtain a reaction product, wherein the molar mass ratio of the beta-cyclodextrin to the citric acid to the disodium hydrogen phosphate is 1:4:1.5; the temperature of the mixed reaction is 780 ℃ and the reaction is 120min.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
example 6
The embodiment provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a composite carrier, wherein the microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the composite carrier comprises clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin. Wherein, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 2:3:2:3:2, the total number of microorganism bacteria is 7.1X10 7 cfu/ml; the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 8:15:20:3, the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 30wt%.
Wherein, the clamshell powder-cellulose acetate foam powder is prepared by the following steps: mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture, wherein the mass ratio of the clamshell powder to the cellulose acetate is as follows: 28:12; and (3) carrying out melt extrusion and foaming on the mixture, the polypropylene and the foaming agent to obtain the clamshell powder-cellulose acetate foaming powder, wherein the mass ratio of the mixture to the polypropylene to the foaming agent is 40:10:5, a step of; the temperature of the melt extrusion is 150 ℃, and the melt blending is carried out for 10min; foaming temperature is 200 ℃, foaming is carried out for 10min; the foaming agent is azodicarbonamide.
The cyclodextrin is citric acid modified cyclodextrin, and the citric acid modified cyclodextrin is prepared by the following steps: mixing beta-cyclodextrin, citric acid and disodium hydrogen phosphate for reaction, precipitating and washing to obtain a reaction product, wherein the molar mass ratio of the beta-cyclodextrin to the citric acid to the disodium hydrogen phosphate is 1:3:1.2; the temperature of the mixed reaction was 75℃for 80min.
The microbial preparation provided by the embodiment is prepared by the following steps:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the composite carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
comparative example 1
The comparative example provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a carrier, wherein the microorganisms comprise saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria, the carrier is calcium carbonate powder, and the ratio of the number of viable bacteria of the saccharomycetes, the lactobacillus, the actinomycetes, the bacillus subtilis and the nitrifying bacteria is 1:3:2:2:1.
the preparation method comprises the following steps: respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
comparative example 2
The comparative example provides a microbial preparation for surface water pollutant treatment, which comprises microorganisms and a carrier, wherein the microorganisms comprise saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria, the carrier is calcium carbonate powder, and the ratio of the number of viable bacteria of the saccharomycetes, the lactobacillus, the actinomycetes, the bacillus subtilis and the nitrifying bacteria is 1:1.5:1:1:0.5.
the preparation method comprises the following steps: respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
uniformly mixing the fermentation liquor and the carrier to obtain a microbial preparation; the mass ratio of the fermentation liquor to the composite carrier is 1:2.
application and test: the prepared microbial preparation is added into polluted surface water (the COD concentration in the polluted surface water in the test is 102mg/L, the total nitrogen concentration is 6.4mg/L, the total phosphorus concentration is 3.6mg/L and the algae concentration is 32.1 mg/L), and the addition amount is 1.5% of the volume of the polluted surface water; the treatment time was 7 days. The COD in the sewage before and after the treatment of the test method is measured by adopting a potassium dichromate method, the total nitrogen concentration in the sewage before and after the treatment of the test method is measured by adopting a potassium persulfate oxidation-ultraviolet spectrophotometry, and the total phosphorus in the sewage before and after the treatment of the test method is measured by adopting an ammonium molybdate spectrophotometry; the algae in the sewage before and after the treatment of the test method are measured by a microscope. The test data are shown in Table 1.
TABLE 1
As can be seen from Table 1, the removal rates of algae, total nitrogen, total phosphorus and COD in examples 1-6 reach 95-99%, 94-99%, 89-97%, 92-95%, respectively, the content of COD, total nitrogen and total phosphorus in the treated polluted river water reaches the quality standard of surface water environment (GB 3838-2002) class III water quality standard, and the content indexes of COD, ammonia nitrogen and total phosphorus in the treated sewage water reach the first grade B standard of pollutant emission standard of urban sewage treatment plant (GB 18918-2002). And the removal rates of the algae, the total nitrogen, the total phosphorus and the COD in the comparative examples 1 and 2 reach 79 to 81 percent, 72 to 78 percent, 61 to 69 percent and 77 to 80 percent respectively. Thus, examples 1-6 are significantly better than comparative examples 1 and 2.

Claims (10)

1. The microbial preparation for surface water pollutant treatment is characterized in that: the composite microbial preparation comprises composite microorganisms and a composite carrier solution, wherein the composite microorganisms comprise saccharomycetes, lactic acid bacteria, actinomycetes, bacillus subtilis and nitrifying bacteria, and the composite carrier solution is an aqueous solution comprising clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin.
2. A microbial preparation according to claim 1, characterized in that: the total number of viable microorganisms in the composite microorganism is more than 5 multiplied by 10 7 cfu/ml, the ratio of the number of viable bacteria of saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria is 1-2:3-4:2-3:2-3:1-2.
3. A microbial preparation according to claim 1, characterized in that: the mass ratio of the clamshell powder to the cellulose acetate foaming powder to the brown granulated sugar to the chitosan to the cyclodextrin is 5-10:10-20:15-25:1-5, wherein the total mass fraction of the clamshell powder-cellulose acetate foam powder, the brown granulated sugar, the chitosan and the cyclodextrin is 25-40wt%.
4. A microbial preparation according to claim 1, characterized in that: the clamshell powder-cellulose acetate foam powder is prepared by the following steps:
mixing and grinding the clamshell powder and the cellulose acetate to obtain a mixture;
and (3) melting, extruding and foaming the mixture, polypropylene and a foaming agent to obtain the clamshell powder-cellulose acetate foaming powder.
5. The microbial preparation according to claim 4, wherein: the mass ratio of the clamshell powder to the cellulose acetate is as follows: (25-30): (10-15); the mass ratio of the mixture to the polypropylene to the foaming agent is (35-45): (5-15): 1-8; the temperature of the melt extrusion is 140-160 ℃, and the melt blending is carried out for 8-12min; foaming temperature is 180-210 deg.C, foaming is 5-20min.
6. A microbial preparation according to claim 1, characterized in that: the cyclodextrin is citric acid modified cyclodextrin, and the citric acid modified cyclodextrin is prepared by the following steps: beta-cyclodextrin, citric acid and disodium hydrogen phosphate are mixed for reaction, and the precipitate is washed to obtain a reaction product.
7. The microbial preparation according to claim 6, wherein: the molar mass ratio of the beta-cyclodextrin to the citric acid to the disodium hydrogen phosphate is 1: (2-4): (1-1.5); the temperature of the mixing reaction is 70-80 ℃ and the reaction lasts for 60-120 min.
8. A process for preparing a microbial preparation according to any one of claims 1 to 7, characterized by comprising the steps of:
mixing and stirring clamshell powder-cellulose acetate foam powder, brown granulated sugar, chitosan and cyclodextrin to obtain a composite carrier;
respectively inoculating saccharomycetes, lactobacillus, actinomycetes, bacillus subtilis and nitrifying bacteria into the culture solution for fermentation culture, and mixing to obtain fermentation liquor;
and uniformly mixing the fermentation liquor and the composite carrier to obtain the microbial preparation.
9. The method according to claim 8, wherein the mass ratio of the fermentation broth to the composite carrier is 1: (1.5-3).
10. Use of the microbial preparation of any one of claims 1-7 in sewage treatment.
CN202311474253.8A 2023-11-08 2023-11-08 Microbial preparation for surface water pollutant treatment, preparation method and application Pending CN117187088A (en)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101082029A (en) * 2006-06-01 2007-12-05 上海泓宝绿色水产科技发展有限公司 Preparation of water purification bacterium and method for restoring aquaculture environment
CN106148246A (en) * 2016-08-30 2016-11-23 蓝德环保科技集团股份有限公司 Purify compounding microbial inoculum of black and odorous water and preparation method thereof
CN106145375A (en) * 2015-04-17 2016-11-23 南京莎菲特生物科技有限公司 A kind of preparation and application method for removing sewage and the compositions educating river course again
CN106673165A (en) * 2015-11-06 2017-05-17 周坤友 High-molecular chitosan composite flocculant
CN107500419A (en) * 2017-09-28 2017-12-22 合肥市东方美捷分子材料技术有限公司 One kind administers black and odorous water sustained release QQ balls and its processing method and application
CN111115843A (en) * 2019-12-25 2020-05-08 安徽麓瑞科技有限公司 Combined water quality improver for freshwater fish culture pond
CN114746541A (en) * 2019-12-16 2022-07-12 4D制药研究有限公司 Providing bacterial biomass with improved storage stability

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101082029A (en) * 2006-06-01 2007-12-05 上海泓宝绿色水产科技发展有限公司 Preparation of water purification bacterium and method for restoring aquaculture environment
CN106145375A (en) * 2015-04-17 2016-11-23 南京莎菲特生物科技有限公司 A kind of preparation and application method for removing sewage and the compositions educating river course again
CN106673165A (en) * 2015-11-06 2017-05-17 周坤友 High-molecular chitosan composite flocculant
CN106148246A (en) * 2016-08-30 2016-11-23 蓝德环保科技集团股份有限公司 Purify compounding microbial inoculum of black and odorous water and preparation method thereof
CN107500419A (en) * 2017-09-28 2017-12-22 合肥市东方美捷分子材料技术有限公司 One kind administers black and odorous water sustained release QQ balls and its processing method and application
CN114746541A (en) * 2019-12-16 2022-07-12 4D制药研究有限公司 Providing bacterial biomass with improved storage stability
CN111115843A (en) * 2019-12-25 2020-05-08 安徽麓瑞科技有限公司 Combined water quality improver for freshwater fish culture pond

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Application publication date: 20231208