CN117184652B - Esophageal cancer diagnosis kit and diagnosis method - Google Patents

Abstract

The invention belongs to the technical field of kits, and particularly relates to an esophageal cancer diagnosis kit and a diagnosis method. The reagent box comprises a box body, wherein a partition board is longitudinally arranged in the box body, the partition board divides the box body into a reagent storage area and a reaction area, a plurality of baffles are arranged in the reagent storage area, the baffles divide the reagent storage area into a plurality of storage chambers, and a reagent bottle is placed in each storage chamber; the reagent bottle is provided with a sample adding component and a sample discharging component; the sample adding component comprises a rotating body, a supporting body and a movable body. The esophageal cancer diagnosis kit provided by the invention can realize convenient sample adding when detecting samples.

Description

Esophageal cancer diagnosis kit and diagnosis method

Technical Field

The invention belongs to the technical field of kits, and particularly relates to an esophageal cancer diagnosis kit and a diagnosis method.

Background

Esophageal cancer is a common digestive tract tumor, and typical symptoms of esophageal cancer are progressive dysphagia, firstly, food difficult to dry in throat, then semifluid food, and finally, water and saliva cannot be swallowed. The detection method of esophageal cancer comprises imaging examination such as gas-barium dual contrast radiography, CT examination and magnetic resonance examination; endoscopy, such as plain white light fiber endoscopy, pigment endoscopy, ultrasonic endoscopy; laboratory tests such as blood routine, liver and kidney function, blood glucose, detection of tumor markers, etc. Among the most common means of laboratory testing is kit testing.

There are many cases related to esophageal cancer diagnosis kits in the prior art, such as CN101608218A, CN109541213A, CN208156014U, and the innovation points of these patents are the improvement of the reagent formulation or the structure of the kit. For example, CN208156014U provides a kit for detecting esophageal cancer drug sensitivity, which comprises a kit, a box cover, a box button and a reagent, wherein the box cover is embedded above the kit, the box button is embedded in the middle surface of the kit, the box button is fixedly connected with the box cover above the box button, reagents in a strip shape are uniformly distributed inside the kit, a second kit is embedded above the kit, a fixed slot is embedded below the second kit, clamping slots are embedded on the left side and the right side of the inside of the fixed slot, a transmission base is embedded below the reagent, and a fixed plate is embedded above the inside of the kit; this patent stable in structure, the convenience is used reagent, can improve the use course of treatment and deadline, can improve the fixity of kit to conveniently take out reagent, have very high spreading value.

The structure of the kit is closely related to the convenience of the detection method of the kit. The kit structure provided by CN208156014U only provides convenience in terms of installing reagent bottles and carrying reagents, however, how to realize convenient sample addition is a concern for at least two kinds of kit detection reagents. In order to realize convenient sample adding during sample detection, the invention provides a novel kit structure.

Disclosure of Invention

In order to solve the technical problems, the invention provides an esophageal cancer diagnosis kit which can realize convenient sample adding when detecting a sample.

The invention aims to provide an esophageal cancer diagnosis kit, which comprises a kit body, wherein a partition board is longitudinally arranged in the kit body, the partition board divides the kit body into a reagent storage area and a reaction area, a plurality of baffles are arranged in the reagent storage area, the baffles divide the reagent storage area into a plurality of storage chambers, and a reagent bottle is placed in each storage chamber;

the reagent bottle is provided with a sample adding component and a sample discharging component;

the sample adding assembly comprises a rotating body, a supporting body and a moving body, wherein the rotating body is rotationally connected to the bottle mouth of the reagent bottle, the moving body and the supporting body are both arranged in the reagent bottle, the rotating body is movably connected with the moving body, so that the moving body can move up and down along the rotating body, the lower end of the moving body can move to enter the sample discharging assembly, and the tube mouth of the sample discharging assembly faces the reaction area.

Preferably, in the above esophageal cancer diagnosis kit, the reagent bottle comprises a bottle body and a bottle cap which are detachably connected, a mounting opening is formed in the bottle cap, and the rotator is rotatably connected to the mounting opening.

Preferably, in the above esophageal cancer diagnosis kit, the support body is a rod vertically arranged in the bottle body;

the movable body comprises a first connecting rod, the upper end of the first connecting rod is rotationally connected with the bottom of the movable body, the lower end of the first connecting rod is connected with the upper end of a second connecting rod through a rotating piece, the rotating piece is vertically movably connected to the support body, the upper end of a third connecting rod is fixedly connected with the lower end of the second connecting rod, and the lower end of the third connecting rod is opposite to the sample outlet assembly and can extend into the sample outlet assembly.

Preferably, in the above esophageal cancer diagnosis kit, the rotating member includes a first movable block, a vertical chute is formed in the support body, the first movable block is slidably disposed in the chute, a first telescopic member is connected between the first movable block and the bottom in the chute, one surface of the first movable block facing the movable body is provided with a first connecting member, and the first connecting member is respectively connected with the first connecting rod and the second connecting rod in a rotating manner.

Preferably, in the above esophageal cancer diagnosis kit, the first telescopic member is a spring or an elastic air bag.

Preferably, in the esophageal cancer diagnostic kit, the sample outlet component is a section of tube, a tube orifice at one end of the tube is connected and communicated with the lower end of the side wall of the bottle body, a tube orifice at the other end of the tube faces the reaction area, the lower end of the third connecting rod is rotationally connected with the sealing plate, and the sealing plate is rotationally arranged in the sample outlet component and can seal the sample outlet component.

Preferably, in the above esophageal cancer diagnostic kit, the sample outlet component is a section of curved tube, one end of the sample outlet component connected with the bottle body is horizontal or inclined downward, and one end of the sample outlet component facing the reaction zone is horizontal or inclined upward.

Preferably, in the esophageal cancer diagnosis kit, a supporting rod is rotatably arranged at the bottom of the rotator, the lower end of the supporting rod is rotatably connected to the top of a piston plate, and the piston plate is in contact with the inner wall of the bottle body;

the support body is a support plate which is horizontally arranged and is fixed at the inner bottom of the bottle body;

the movable body comprises a fourth connecting rod and a fifth connecting rod, the upper end of the fourth connecting rod is rotationally connected with the bottom of the rotating body, the lower end of the fourth connecting rod is connected with the fifth connecting rod through a sliding piece, and the lower end of the fifth connecting rod is opposite to the sample outlet assembly.

Preferably, in the above esophageal cancer diagnosis kit, the piston plate is provided with a through hole, and the fourth link rod penetrates through the through hole.

The invention provides a method for detecting tumor indexes by using the esophageal cancer diagnostic kit, which comprises the following steps:

selecting an index type to be detected;

selecting reagents for detecting indexes, correspondingly filling each reagent into a reagent bottle, and mixing the reagent with a tumor sample by using the esophageal cancer diagnosis kit;

and detecting the index content by an instrument after the reaction.

Compared with the prior art, the invention has the following beneficial effects:

the reagent bottle is provided with a sample adding component and a sample discharging component, and after the sample adding component is operated, reagent liquid in the reagent bottle can be added into the reaction zone through the sample discharging component. The sample adding components on different reagent bottles are independently operated and do not interfere with each other, namely, the sample adding component on one reagent bottle is operated, and the sample adding components on the rest reagent bottles are not operated. The linkage of the movable body and the closing plate is realized by operating the rotating body, so that the operation is convenient, and other pipettors are not required to be contacted. The rotating angle of the rotating body and the opening time of the joint of the sample outlet assembly and the reagent bottle are designed, the rotating body is rotated once, and then reagent can be quantitatively added into the reaction area.

The sample outlet component is in a bent tubular shape, one end of the sample outlet component connected with the bottle body is horizontal or inclined downwards, one end of the sample outlet component facing the reaction zone is horizontal or inclined upwards, and then the reagent flows out of the sample outlet component and enters the reaction zone, and even if the reagent splashes, the reagent cannot splash back into the sample outlet component, and the sample outlet component and the corresponding reagent bottle are placed to pollute the reagent.

Drawings

FIG. 1 is a schematic perspective view of a diagnostic kit for esophageal cancer according to example 1 of the invention.

FIG. 2 is a cross-sectional view of the esophageal cancer diagnosis kit of example 1 of the invention after being slit from the reaction zone.

FIG. 3 is a plan view showing the internal structure of the esophageal cancer diagnostic kit of example 1 of the invention.

FIG. 4 is a diagram showing the connection relationship between the reagent bottle and the internal parts thereof (i.e., the left side view of one storage compartment of FIG. 1) according to the embodiment 1 of the present invention.

Fig. 5 is a schematic diagram of the connection between the rotating member and the supporting body in embodiment 1 of the present invention (i.e. the right side view of the connection structure between the rotating member and the supporting body in fig. 4).

Fig. 6 is a schematic diagram of the operation of the reagent bottle and its internal components (i.e. the operation state diagram of fig. 4) according to the embodiment 1 of the present invention.

FIG. 7 is a diagram showing the connection relationship between the reagent bottle and the internal parts thereof according to example 2 of the present invention.

Fig. 8 is a top view of the piston plate of example 2 of the present invention.

Detailed Description

In order that those skilled in the art will better understand the technical scheme of the present invention, the present invention will be further described with reference to specific embodiments and drawings.

In the description of the present invention, unless otherwise specified, all reagents are commercially available and methods are conventional in the art.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.

The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second" may include one or more such features, either explicitly or implicitly; in the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.

Example 1

Referring to fig. 1-6, the esophageal cancer diagnosis kit comprises a box body 1, wherein the outside of the box body 1 is in a three-dimensional structure such as a cube, a cylinder or an elliptic cylinder, and the like, and the stability is good when the bottom surface of the box body 1 is a horizontal surface and is placed on a detection table. The case 1 is made of transparent materials, so that the residual amount of the internal reagent and the reaction condition can be conveniently observed. For example, the detachable shading cloth is wrapped outside the box body 1, and for the reaction requiring shading, the shading cloth can be covered on the box body 1 first, and then the sample is added for reaction. The top of the box body 1 is provided with a box cover, and the box cover is hinged with the top of the side wall of the box body 1, or the box cover is buckled and connected with the top of the box body 1.

The inside of the box body 1 is longitudinally provided with a partition board 2, and the partition board 2 may or may not be transparent, and illustratively, the partition board 2 is vertically arranged in the box body 1. The partition board 2 divides the box body 1 into a reagent storage area and a reaction area, the reagent storage area is used for placing a reagent bottle 3, the reagent in the reagent bottle 3 is a reagent capable of diagnosing esophageal cancer, and the reaction area is used for detecting reaction, and as a plurality of indexes are detected at normal temperature and normal pressure, the detection can be directly carried out in the reaction area. Of course, a reaction tank can be arranged in the reaction zone, the reaction tank can be taken out from the reaction zone, the reagent and the sample are added into the reaction tank, then the reaction tank is moved into a reaction box with proper temperature for reaction, and finally, the reaction result is observed, so that the esophageal cancer is diagnosed. The reaction zone is internally provided with a sample adding port, or the upper end of the reaction zone is open.

Illustratively, a plurality of baffles 4 are disposed within the reagent storage area, the baffles 4 dividing the reagent storage area into a plurality of storage compartments, each of which houses one reagent bottle 3.

The reagent bottle 3 is provided with a sample adding component 5 and a sample outputting component 6, and after the sample adding component 5 is operated, reagent liquid in the reagent bottle 3 can be added into the reaction zone through the sample outputting component 6. The sample adding components 5 on different reagent bottles 3 are independently operated and do not interfere with each other, namely, the sample adding components 5 on one reagent bottle 3 are operated, and the sample adding components 5 on the rest reagent bottles 3 are not operated.

Referring to fig. 2 to 5, the sample loading unit 5 comprises a rotary body 51, a supporting body 52 and a movable body 53, wherein the rotary body 51 is rotatably connected to the mouth of the reagent bottle 3, that is, the rotary body 51 is positioned at the top of the cartridge 1, which is convenient for a person to operate. The movable body 53 and the supporting body 52 are positioned below the rotary body 51, and the movable body 53 and the supporting body 52 are both arranged in the reagent bottle 3. The rotating body 51 is movably connected with the movable body 53 such that the movable body 53 can move up and down along the rotating body 51. The movable body 53 is rotatably connected with the rotary body 51, and when the rotary body 51 reciprocally rotates, the movable body 53 can be carried to move up and down, and the lower end of the movable body 53 can move into the sample outlet assembly 6.

The reagent bottle 3 includes a bottle body 31 and a bottle cap 32, the bottle cap 32 is buckled on or inserted into the bottle body 31, the bottle cap 32 is opened to supplement reagent into the bottle body 31, and the rotator 51 can be operated after the bottle cap 32 is covered. The bottle cap 32 is provided with a mounting opening, and the rotator 51 is rotatably connected to the mounting opening. For example, the rotator 51 is a cylinder transversely arranged, the cylinder is located in the mounting opening, two ends of the cylinder along the axial direction are respectively connected with a rotating shaft, the central shaft of the rotating shaft coincides with the central shaft of the cylinder, and the other end of the rotating shaft is mounted at the edge of the mounting opening. Both the movable body 53 and the supporting body 52 are located in the body 31.

Illustratively, the support 52 is a rod vertically disposed within the body 31 of the reagent bottle 3.

Illustratively, the movable body 53 includes a first link 531, a second link 532, and a third link 533, where an upper end of the first link 531 is rotatably connected to a bottom of the movable body 53, for example, by a hinge or a hinge. The lower end of the first link 531 is connected to the upper end of the second link 532 by a rotating member 534, the rotating member 534 is a member having a rotating function such as a hinge or a hinge, the rotating member 534 is vertically movably connected to the supporting body 52, and at this time, the supporting body 52 is fixed to the inner bottom of the test bottle body 31. The upper end of the third link 533 is fixedly connected to the lower end of the second link 532, and the lower end of the third link 533 is opposite to the sample ejection assembly 6. When the rotator 51 is rotated clockwise from the position shown in fig. 4 to the position shown in fig. 6, the first link 531 is driven to move clockwise, the distance between the connection point of the first link 531 and the rotator 51 and the support 52 becomes shorter gradually, and the rotator 534 connected to the first link 531 moves downward along the support 52 and then moves downward with the second link 532 because the first link 531 cannot expand and contract. Referring to fig. 6, the second link 532, the third link 533, the supporting body 52 and the bottom of the reagent bottle 3 are surrounded by a triangle-like structure, and when the upper end of the second link 532 moves downward, the third link 533 is pushed toward the sample ejection assembly 6, and the angle of the second link 532 is slightly changed.

The structure of the rotating member 534, as illustrated in fig. 5, includes a first movable block 5341, a first telescopic member 5342, and a first connecting member 5343, where the supporting body 52 is provided with a vertical chute 521, the first movable block 5341 is located in the chute 521, the first movable block 5341 is slidably disposed in the chute 521, the first telescopic member 5342 is a member with an elastic function such as a spring or an elastic air bag, and the first telescopic member 5342 is located in the chute 521, and an upper end of the first telescopic member 5342 is fixedly connected with the first movable block 5341, a lower end of the first telescopic member 5342 is fixedly connected with an inner bottom of the chute 521, a first connecting member 5343 is fixed on a surface of the first movable block 5341 facing the movable body 53, and a first connecting rod 531 and a second connecting rod 532 are respectively rotatably connected to the first connecting member 5343. The first connecting element 5343 and the first movable block 5341 are both block-shaped structures.

When the rotor 51 is rotated to force the first movable block 5341 to move downward, the first expansion member 5342 is compressed, the movable body 53 moves toward the sample outlet assembly 6, and the reagent in the bottle body 31 flows to the sample outlet assembly 6, so that the reagent is added to the reaction area. When the external force disappears, the first telescopic piece 5342 can automatically restore to the original position, the rotating body 51 also automatically resets, the movable body 53 moves back to the sample outlet assembly 6, the reagent cannot circulate, and the reagent addition in the reaction area is stopped. Under the above-mentioned rotating member 534 structure, when needing to add reagent, only need rotate the rotor 51, when needing to stop the application of sample, cancel to the application of force of rotor 51 can, convenient operation.

Illustratively, the sample outlet assembly 6 is a section of tube, one end orifice of the tube is connected with the lower end of the side wall of the bottle body 31 of the reagent bottle 3, the bottle body 31 is communicated with the sample outlet assembly 6, and the other end orifice of the sample outlet assembly 6 faces the reaction zone. The sample ejection assembly 6 is provided with a closing plate 61 rotatably inside the end connected to the reagent bottle 3. For example, the bottom of the sample ejection assembly 6 is rotatably connected to the bottom of the closing plate 61, and the third link 533 is connected to the top of the closing plate 61. The closing plate 61 can be rotated to close the joint of the sample outlet assembly 6 and the reagent bottle 3, so that the liquid does not circulate, and the closing plate 61 can also be rotated to open the joint of the sample outlet assembly 6 and the reagent bottle 3, so that the liquid circulates.

The sealing ring is sleeved on the periphery of the sealing plate 61, and when the sealing plate 61 is positioned for sealing the sample outlet assembly 6, the sealing ring is abutted against the inner wall of the sample outlet assembly 6, so that the sealing effect is good. The lower end of the movable body 53 is rotatably connected to the closing plate 61, such as hinged or hinged, that is, the lower end of the third link 533 is rotatably connected to the closing plate 61. When the movable body 53 moves downwards, the lower end of the movable body 53 pushes against the sealing plate 61, the sealing plate 61 is forced to rotate until the connection part of the sample outlet assembly 6 and the reagent bottle 3 is opened, and when the movable body 53 moves upwards, the lower end of the movable body 53 pulls against the sealing plate 61, and the sealing plate 61 is forced to rotate until the connection part of the sample outlet assembly 6 and the reagent bottle 3 is sealed. Because the appearance subassembly 6 is connected with the body 31 lateral wall lower extreme of reagent bottle 3, and reagent liquid level is higher than appearance subassembly 6 in the reagent bottle 3, opens when appearance subassembly 6 and reagent bottle 3 junction, and the gravity effect of reagent makes liquid flow out from appearance subassembly 6, and gets into in the reaction zone.

The movable body 53 and the closing plate 61 are linked by operating whether the rotating body 51 rotates or not, so that the operation is convenient. By designing the rotation angle of the rotating body 51 and the opening time of the joint of the sample outlet assembly 6 and the reagent bottle 3, the reagent can be quantitatively added into the reaction area by rotating the rotating body 51 once. Without touching other pipettes.

The sample outlet assembly 6 is exemplified by a section of a curved tube, the end of the sample outlet assembly 6 connected with the bottle body 31 is horizontal or inclined downwards, the end of the sample outlet assembly 6 facing the reaction zone is horizontal or inclined upwards, for example, the sample outlet assembly 6 is arranged in a Z shape, and after the reagent flows out of the sample outlet assembly 6 into the reaction zone, the reagent can not splash back into the sample outlet assembly 6 even if splashes, and the sample outlet assembly 6 and the corresponding reagent bottle 3 are placed to be polluted.

Example 2

An esophageal cancer diagnosis kit is basically the same as the structure of embodiment 1, except that referring to fig. 7-8, a support rod 511 and a piston plate 512 are further provided at the bottom of the rotator 51, the upper end of the support rod 511 is rotatably connected to the bottom of the rotator 51, the lower end of the support rod 511 is rotatably connected to the top of the piston plate 512, the piston plate 512 is slidably connected to the inner wall of the body 31, and the piston plate 512 has a piston function. That is, when the rotator 51 rotates, the supporting rod 511 is driven to rotate and move downwards, and the piston plate 512 can only move downwards due to the fact that the piston plate is connected with the body of the reagent bottle 3 in a sliding manner, so that the reagent in the reagent bottle can be pushed out from the sample outlet assembly 6, and the sample adding speed can be improved.

On the other hand, the support body 52 is a horizontally disposed support plate fixed to the inner bottom of the bottle body 31. The movable body 53 includes a fourth link 535 and a fifth link 536, and an upper end of the fourth link 535 is rotatably connected to a bottom of the rotary body 51, for example, by a hinge or a hinge. The lower end of the fourth link 535 is connected to the fifth link 536 by a slider 537, and the lower end of the fifth link 536 is opposite the sample ejection assembly 6.

The piston plate 512 is provided with a through hole 5121, the fourth connecting rod 535 penetrates through the through hole 5121, and in order to avoid the through hole 5121 affecting the piston function of the piston plate 512, a sealing sleeve is arranged at the inner edge of the through hole 5121, for example, the sealing sleeve is a sleeve with a sealing function such as a leather sleeve, the sealing sleeve is sleeved on the periphery of the fourth connecting rod 535, and the sealing sleeve has a sealing function and does not hinder the movement of the fourth connecting rod 535.

The slider 537 has the same structure as the rotator 534 except that the direction of the slider 537 is laterally set. Specifically, slider 537 includes second movable block, second extensible member, second connecting piece, has seted up horizontal slide on the support body 52, and the second movable block embedding sets up in the slide in a sliding way, and the second extensible member is spring or elasticity gasbag, and the second extensible member is located the slide to, the one end and the second movable block fixed connection of second extensible member, the other end and the interior bottom fixed connection of slide of second extensible member, the one side of second movable block towards the movable body 53 is fixed with the second connecting piece, rotates on the second connecting piece respectively and is connected with fourth connecting rod 535 and fifth connecting rod 536.

Similar to the principle of embodiment 1, when the rotator 51 is rotated to force the lower end of the fourth link 535 to move to the right in fig. 7, the second movable block is also moved, the second telescopic member is compressed, and the fifth link 536 moves toward the sample ejection assembly 6, so that the reagent flows and the reagent is added. When the external force disappears and the second telescopic member can automatically restore to the original position, the rotator 51 is also automatically restored, and the fifth connecting rod 536 moves back to the sample outlet assembly 6, so that the reagent cannot circulate. With the slider 537 described above, when sample application is required, the rotator 51 is only required to be rotated, and when sample application is required to be stopped, the force applied to the rotator 51 is canceled.

It should be noted that, the connection relationships of the components not specifically mentioned in the present invention are all default to the prior art, and the connection relationships of the structures are not described in detail because they do not relate to the invention points and are common applications of the prior art.

It should be noted that, when numerical ranges are referred to in the present invention, it should be understood that two endpoints of each numerical range and any numerical value between the two endpoints are optional, and because the adopted step method is the same as the embodiment, in order to prevent redundancy, the present invention describes a preferred embodiment. While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the invention.

It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims (5)

1. The esophageal cancer diagnosis kit is characterized by comprising a kit body (1), wherein a partition board (2) is longitudinally arranged in the kit body (1), the partition board (2) divides the kit body (1) into a reagent storage area and a reaction area, a plurality of baffles (4) are arranged in the reagent storage area, the baffles (4) divide the reagent storage area into a plurality of storage chambers, and a reagent bottle (3) is placed in each storage chamber;

the reagent bottle (3) is provided with a sample adding component (5) and a sample discharging component (6);

the sample adding assembly (5) comprises a rotating body (51), a supporting body (52) and a movable body (53), wherein the rotating body (51) is rotationally connected to the bottle mouth of the reagent bottle (3), the movable body (53) and the supporting body (52) are both arranged in the reagent bottle (3), the rotating body (51) is movably connected with the movable body (53), so that the movable body (53) can move up and down along the rotating body (51), the lower end of the movable body (53) can move into the sample outlet assembly (6), and the nozzle of the sample outlet assembly (6) faces the reaction zone;

the reagent bottle (3) comprises a bottle body (31) and a bottle cap (32) which are detachably connected, a mounting opening is formed in the bottle cap (32), and the rotator (51) is rotationally connected to the mounting opening;

the support body (52) is a rod vertically arranged in the bottle body (31);

the movable body (53) comprises a first connecting rod (531), the upper end of the first connecting rod (531) is rotationally connected with the bottom of the rotating body (51), the lower end of the first connecting rod (531) is connected with the upper end of a second connecting rod (532) through a rotating piece (534), the rotating piece (534) is vertically movably connected to the supporting body (52), the upper end of a third connecting rod (533) is fixedly connected with the lower end of the second connecting rod (532), and the lower end of the third connecting rod (533) is opposite to the sample outlet assembly (6) and can extend into the sample outlet assembly (6);

the rotating piece (534) comprises a first movable block (5341), a vertical chute (521) is formed in the supporting body (52), the first movable block (5341) is arranged in the chute (521) in a sliding mode, a first telescopic piece (5342) is connected between the first movable block (5341) and the inner bottom of the chute (521), a first connecting piece (5343) is arranged on one face, facing the movable body (53), of the first movable block (5341), and the first connecting piece (5343) is connected with the first connecting rod (531) and the second connecting rod (532) in a rotating mode respectively.

2. The esophageal cancer diagnostic kit of claim 1, wherein the first telescoping member (5342) is a spring or an elastic balloon.

3. The esophageal cancer diagnosis kit according to claim 1, wherein the sample outlet assembly (6) is a tube, one end opening of the tube is connected and communicated with the lower end of the side wall of the bottle body (31), the other end opening of the tube faces the reaction area, the lower end of the third connecting rod (533) is rotatably connected with the sealing plate (61), and the sealing plate (61) is rotatably arranged in the sample outlet assembly (6) and can seal the sample outlet assembly (6).

4. The esophageal cancer diagnostic kit according to claim 1, wherein the sample-discharging member (6) is a curved tubular section, the end of the sample-discharging member (6) connected to the bottle body (31) is inclined horizontally or downwardly, and the end of the sample-discharging member (6) facing the reaction zone is inclined horizontally or upwardly.

5. A method for detecting an index using the esophageal cancer diagnostic kit according to claim 1, comprising:

selecting an index type to be detected;

selecting reagents for detecting indexes, correspondingly filling each reagent into a reagent bottle (3), and mixing the reagent with a tumor sample by using the esophageal cancer diagnosis kit according to claim 1;

and detecting the index content by an instrument after the reaction.