CN117180997A - Molecular film washing device and application method thereof - Google Patents
Molecular film washing device and application method thereof Download PDFInfo
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- CN117180997A CN117180997A CN202311156800.8A CN202311156800A CN117180997A CN 117180997 A CN117180997 A CN 117180997A CN 202311156800 A CN202311156800 A CN 202311156800A CN 117180997 A CN117180997 A CN 117180997A
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- 238000005406 washing Methods 0.000 title claims abstract description 47
- 239000002120 nanofilm Substances 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000011534 incubation Methods 0.000 claims abstract description 96
- 239000007788 liquid Substances 0.000 claims abstract description 89
- 239000002699 waste material Substances 0.000 claims abstract description 80
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 57
- 238000011084 recovery Methods 0.000 claims abstract description 28
- 239000000463 material Substances 0.000 claims abstract description 23
- 239000012528 membrane Substances 0.000 claims description 11
- 239000011148 porous material Substances 0.000 claims description 4
- 239000003593 chromogenic compound Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 239000000243 solution Substances 0.000 description 20
- 230000000694 effects Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000000967 suction filtration Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 239000006180 TBST buffer Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
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Abstract
The application provides a molecular film washing device and a using method thereof, and the technical scheme is as follows: comprising the following steps: incubating the tank body; a reagent tank and a solution recovery tank are arranged in the incubation tank body; a microporous filter plate; the microporous filter plate is arranged in the incubation groove body and is positioned above the reagent groove; a waste liquid pipe; one end of the waste liquid pipe penetrates through the microporous filter plate and then is connected with the reagent tank, and the other end of the waste liquid pipe is connected with a negative pressure pump; the solution recovery tank is positioned at one side opposite to the waste liquid pipe; the application has the advantage that the luminescent and color-developing materials remained in the micropores of the film material can be fully removed by an axial washing mode.
Description
Technical Field
The application relates to the technical field of biological treatment equipment, in particular to a molecular film washing device and a using method thereof.
Background
In the molecular detection experiment, a plurality of detection technologies which need to take a film material as a carrier are limited by a film washing step mode and a method, and finally the steps of development or color development and the like often have the problems of weaker film target signals, stronger background signals and the like, and even the high background signals can mask the target signals, so that the sensitivity of the detection technology is greatly reduced.
At present, conventional membrane washing is mainly performed by adopting a horizontal shaking table, an up-and-down shaking table or flowing liquid flushing and the like, and the above-mentioned several washing modes are all membrane horizontal washing modes, and because membrane materials belong to a three-dimensional structure containing micropores instead of a planar structure, molecules and molecular materials used for identifying the molecules can exist in the micropores in a non-binding mode, the horizontal washing mode can lead to the defect that residual luminescent or chromogenic materials in the micropores of the membrane materials cannot be sufficiently washed, so that membrane background signals are higher and target signals are interfered, and therefore, the membrane washing mode needs to be improved.
Disclosure of Invention
Aiming at the defects existing in the prior art, the embodiment of the application provides a molecular film washing device and a use method thereof, so as to solve the problems existing in the related art, and the molecular film washing device has the advantage that luminescent and chromogenic materials remained in micropores of a film material can be sufficiently removed in an axial washing mode. The technical proposal is as follows:
in a first aspect, an embodiment of the present application provides a molecular film washing apparatus, including: incubating the tank body; a reagent tank and a solution recovery tank are arranged in the incubation tank body; a microporous filter plate; the microporous filter plate is arranged in the incubation groove body and is positioned above the reagent groove; a waste liquid pipe; one end of the waste liquid pipe penetrates through the microporous filter plate and then is connected with the reagent tank, and the other end of the waste liquid pipe is connected with a negative pressure pump; the solution recovery tank is positioned at the opposite side of the waste liquid pipe.
In one embodiment, the waste pipe is provided with a waste inlet and a waste outlet; the waste liquid inlet is positioned below the microporous filter plate and is connected with the reagent tank; and the waste liquid outlet is connected with a negative pressure pump.
In one embodiment, the microporous filter plate has a microporous pore size of 1 μm to 1000 μm.
In one embodiment, the solution recovery tank is obliquely arranged on the incubation tank body, and the inclination angle of the incubation tank body is 10-90 degrees.
In one embodiment, the bottom of the solution recovery tank is of a pointed bottom structure and/or a rounded bottom structure.
In one embodiment, the incubation tank is a negative pressure incubation tank.
In one embodiment, the incubation well is a positive pressure incubation well; an incubation groove cover is arranged at the upper end of the positive pressure incubation groove body.
In one embodiment, a positive pressure air pipe is arranged on the positive pressure incubation groove body; one end of the positive pressure air pipe is positioned below the incubation groove cover, and the other end of the positive pressure air pipe is connected with an air pump.
In one embodiment, the incubation well cover is a sealing flexible glue well cover and/or a part of a flexible glue well cover.
In a second aspect, an embodiment of the present application provides a method for using a molecular film washing device, including the steps of:
when the incubation groove body is a negative pressure incubation groove body, the waste liquid outlet is connected with a negative pressure pump, the waste liquid inlet is connected with the reagent groove, and the waste liquid pipe enables the part below the microporous filter plate to generate negative pressure through the negative pressure pump;
when the incubation groove body is a positive pressure incubation groove body, the positive pressure air pipe is connected with the air pump, the waste liquid outlet is connected with the waste liquid bottle and the negative pressure pump, the waste liquid inlet is connected with the reagent groove, and the positive pressure air pipe enables the space between the microporous filter plate and the incubation groove cover to form positive pressure through the air pump;
placing the membrane material on a microporous filter plate in an incubation groove body, adding a washing liquid, and then filtering out the washing liquid by suction;
taking out the film material after washing, adding a luminescent substrate or a chromogenic substrate, incubating for a period of time, and observing a target signal and a background signal by analyzing a gray value or visually comparing the two methods to obtain a developing result;
when waste liquid needs to be removed, the incubation groove body is inclined leftwards, so that the reagent in the incubation groove body flows out into a waste liquid collecting reagent bottle through a waste liquid outlet of a waste liquid pipe;
when the reagent needs to be recovered, the incubation groove body is inclined to the right, the reagent in the incubation groove body flows into the solution recovery groove, and the reagent is recovered in the solution recovery groove by using a pipette.
The technical scheme at least comprises the following beneficial effects:
the molecular film washing device adopts an axial washing mode, and when the film material is subjected to suction filtration washing in the washing device, the luminous or color-developing material remained in micropores of the film material can be sufficiently washed and removed; in addition, the incubation groove body comprises a waste liquid pipe, a reagent groove and a solution recovery groove, so that various different experimental purposes such as reagent temporary storage, reagent recovery requirements, waste liquid removal and the like can be simultaneously met.
The foregoing summary is for the purpose of the specification only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the present application will become apparent by reference to the drawings and the following detailed description.
Drawings
In the drawings, the same reference numerals refer to the same or similar parts or elements throughout the several views unless otherwise specified. The figures are not necessarily drawn to scale. It is appreciated that these drawings depict only some embodiments according to the disclosure and are not therefore to be considered limiting of its scope.
FIG. 1 is a schematic diagram of the structure of a negative pressure incubation tank in the application;
FIG. 2 is a schematic diagram of the structure of the positive pressure incubation tank in the present application;
FIG. 3 is a schematic view of a first use condition of the present application;
fig. 4 is a schematic view of a second use state of the present application.
In the figure: 1. incubating the tank body; 2. a reagent tank; 3. a solution recovery tank; 4. a microporous filter plate; 5. a waste liquid pipe; 6. an incubation groove cover; 7. a positive pressure air tube; 8. a pipette.
Detailed Description
In order that the objects, features and advantages of the application will be readily understood, a more particular description of the application will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings. Several embodiments of the application are presented in the figures. This application may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
In the present application, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances. The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature.
In the present application, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature. The terms "vertical," "horizontal," "left," "right," "up," "down," and the like are used for descriptive purposes only and are not to indicate or imply that the devices or elements being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the application.
Hereinafter, only certain exemplary embodiments are briefly described. As will be recognized by those of skill in the pertinent art, the described embodiments may be modified in various different ways without departing from the spirit or scope of the present application. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive.
The present application will be described in detail below with reference to the accompanying drawings and examples.
Example 1
As shown in fig. 1 and 2, the present embodiment provides a molecular film washing apparatus comprising: incubating the tank body 1; a reagent tank 2 and a solution recovery tank 3 are arranged in the incubation tank body 1; a microporous filter plate 4; the microporous filter plate 4 is arranged in the incubation groove body 1 and is positioned above the reagent groove 2; a waste liquid pipe 5; one end of the waste liquid pipe 5 penetrates through the microporous filter plate 4 and then is connected with the reagent tank 2, and the other end of the waste liquid pipe 5 is connected with a negative pressure pump; the solution recovery tank 3 is located on the opposite side of the waste liquid pipe 5.
In the embodiment, the incubation groove body 1 is provided with a left wall and a right wall, wherein the reagent groove 2 is positioned at the bottom of the incubation groove body 1, the microporous filter plate 4 is arranged in the incubation groove body 1 and positioned above the reagent groove 2, the solution recovery groove 3 is arranged on the right wall and positioned above the microporous filter plate 4, the waste liquid pipe 5 is obliquely arranged on the left wall, one end of the waste liquid pipe 5 penetrates through the microporous filter plate 4 and then is connected with the reagent groove 2, and the other end of the waste liquid pipe is connected with a negative pressure pump connected with the outside;
when the device works, a membrane material is placed on the microporous filter plate 4 in the incubation groove body 1, TBST washing liquid is added into the reagent groove 2, under the action of a negative pressure pump, the pressure above and below the microporous filter plate 4 is different, so that pressure difference is generated, liquid in the reagent groove 2 can flow axially by the pressure difference, the reagent longitudinally passes through the microporous filter plate 4 to achieve suction filtration washing, and the luminous and chromogenic materials remained in micropores of the membrane material can be sufficiently removed by the suction filtration washing, so that the washing effect is achieved.
Further, the waste liquid pipe 5 is provided with a waste liquid inlet and a waste liquid outlet; the waste liquid inlet is positioned below the microporous filter plate 4 and is connected with the reagent tank 2; and the waste liquid outlet is connected with a negative pressure pump.
In this embodiment, the waste liquid outlet is disposed at the upper end of the waste liquid pipe 5 and connected to the negative pressure pump, the waste liquid inlet is disposed at the lower end of the waste liquid pipe 5, and the waste liquid inlet is disposed below the microporous filter plate 4 and connected to the reagent tank 2, so that the liquid in the reagent tank 2 can flow out through the waste liquid pipe 5.
Further, the microporous filter plate 4 has a microporous pore diameter of 1 μm to 1000 μm.
In this embodiment, the microporous filter sheet 4 has a microporous pore diameter of 1 μm to 1000 μm, preferably 10 μm to 100 μm, and the microporous filter sheet 4 does not leak naturally within this range.
Further, the solution recovery tank 3 is obliquely arranged on the incubation tank body 1, and the inclination angle of the incubation tank body 1 is 10-90 degrees.
In this embodiment, the solution recovery tank 3 is obliquely disposed on the right side of the incubation tank 1, and the inclination angle of the incubation tank 1 is 10 ° to 90 °, so that when the incubation tank 1 is integrally inclined rightward, the reagent in the reagent tank 2 can flow into the solution recovery tank 3, and the reagent is recovered in the solution recovery tank 3 by using the pipette, thereby achieving the reagent collection effect.
Further, the bottom of the solution recovery tank 3 is in a pointed bottom structure and/or a round bottom structure.
In this embodiment, the bottom of the solution recovery tank 3 may be provided with a sharp bottom structure or a round bottom structure, so as to collect the waste liquid generated by the incubation tank 1.
Further, the incubation groove body 1 is a negative pressure incubation groove body.
In this embodiment, when the incubation groove body 1 is designed to be negative pressure, the waste liquid outlet of the waste liquid pipe 5 on the incubation groove body is connected with the negative pressure pump, the waste liquid inlet is connected with the reagent groove, and the negative pressure is generated in the reagent groove by the negative pressure pump, so that the pressure above the microporous filter plate 4 is greater than the pressure below the microporous filter plate 4, the pressure difference is generated at the upper and lower positions of the microporous filter plate 4, and the effect that the liquid can flow axially in the incubation groove body 1 is achieved.
Further, the incubation groove body 1 is a positive pressure incubation groove body; an incubation groove cover 6 is arranged at the upper end of the positive pressure incubation groove body. A positive pressure air pipe 7 is arranged on the positive pressure incubation groove body 1; one end of the positive pressure air pipe 7 is positioned below the incubation groove cover 6, and the other end of the positive pressure air pipe 7 is connected with an air pump.
In this embodiment, when the incubation groove body 1 is designed to be positive pressure, an incubation groove cover 6 is arranged at the upper end of the incubation groove body 1, a positive pressure chamber is formed between the incubation groove cover 6 and the microporous filter plate 4, a positive pressure air pipe 7 is arranged below the incubation groove cover 6 and is communicated with the positive pressure chamber, the other end of the positive pressure air pipe 7 is connected with an air pump, and positive pressure can be generated in the positive pressure chamber through the air pump; the waste liquid outlet of the waste liquid pipe 5 on the positive pressure incubation groove body is connected with a negative pressure pump, and the waste liquid inlet is connected with the reagent groove, so that the pressure above the microporous filter plate 4 is higher than the pressure below the microporous filter plate 4, the pressure difference is generated at the upper and lower positions of the microporous filter plate 4, and the effect that liquid can flow axially in the incubation groove body 1 is achieved.
Further, the incubation groove cover 6 is a sealing soft rubber groove cover and/or a part of soft rubber groove cover.
In this embodiment, the incubation groove cover 6 is a sealed soft rubber groove cover or a part of soft rubber groove cover, so that the incubation groove cover 6 is convenient to clean and recover.
Example two
As shown in fig. 3 and 4, the present embodiment provides a use method applied to a molecular film washing device, including the following steps:
when the incubation groove body 1 is a negative pressure incubation groove body, the waste liquid outlet is connected with a negative pressure pump, the waste liquid inlet is connected with the reagent groove 2, and the waste liquid pipe 5 generates negative pressure on the part below the microporous filter plate 4 through the negative pressure pump;
when the incubation groove body 1 is a positive pressure incubation groove body, the positive pressure air pipe 7 is connected with the air pump, the waste liquid outlet is connected with the waste liquid bottle and the negative pressure pump, the waste liquid inlet is connected with the reagent groove 2, and the positive pressure air pipe enables the space between the microporous filter plate 4 and the incubation groove cover 6 to form positive pressure through the air pump;
placing a membrane material on a microporous filter plate 4 in an incubation groove body 1, adding a washing liquid, and then filtering out the washing liquid by suction;
taking out the film material after washing, adding a luminescent substrate or a chromogenic substrate, incubating for a period of time, and observing a target signal and a background signal by analyzing a gray value or visually comparing the two methods to obtain a developing result;
when waste liquid needs to be removed, the incubation groove body 1 is inclined leftwards, so that the reagent in the incubation groove body 1 flows out into a waste liquid collecting reagent bottle through a waste liquid outlet of the waste liquid pipe 5;
when the reagent needs to be recovered, the inside of the incubation groove body 1 is inclined to the right, the reagent in the incubation groove body flows into the solution recovery groove 3, and the reagent is recovered in the solution recovery groove by using the pipette 8.
The arrow in fig. 3 and 4 indicates the direction of flow of the reagents in the device, i.e. the reagents flow axially in the incubation chamber 1.
The functions of each module in each device can be referred to the corresponding description in the method, and the molecular film washing device and the application method thereof have the advantage that the luminous and chromogenic materials remained in micropores of the film material can be sufficiently removed by an axial washing mode.
The above description is only a preferred embodiment of the present application, and the protection scope of the present application is not limited to the above examples, and all technical solutions belonging to the concept of the present application belong to the protection scope of the present application. It should be noted that modifications and adaptations to the present application may occur to one skilled in the art without departing from the principles of the present application and are intended to be within the scope of the present application.
Claims (10)
1. A molecular film washing apparatus, comprising:
incubating the tank body; a reagent tank and a solution recovery tank are arranged in the incubation tank body;
a microporous filter plate; the microporous filter plate is arranged in the incubation groove body and is positioned above the reagent groove;
a waste liquid pipe; one end of the waste liquid pipe penetrates through the microporous filter plate and then is connected with the reagent tank, and the other end of the waste liquid pipe is connected with a negative pressure pump; the solution recovery tank is positioned at the opposite side of the waste liquid pipe.
2. The molecular film washing apparatus according to claim 1, wherein the waste liquid pipe has a waste liquid inlet and a waste liquid outlet; the waste liquid inlet is positioned below the microporous filter plate and is connected with the reagent tank; and the waste liquid outlet is connected with a negative pressure pump.
3. The molecular film washing apparatus according to claim 1, wherein the microporous filter plate has a microporous pore diameter of 1 μm to 1000 μm.
4. The molecular film washing apparatus according to claim 1, wherein the solution recovery tank is disposed obliquely on the incubation tank, and the angle of inclination of the incubation tank is 10 ° to 90 °.
5. The molecular film washing apparatus according to claim 4, wherein the bottom of the solution recovery tank has a pointed bottom structure and/or a rounded bottom structure.
6. The molecular film washing apparatus according to any one of claims 1 to 5, wherein the incubation chamber is a negative pressure incubation chamber.
7. The molecular film washing apparatus according to any one of claims 1 to 5, wherein the incubation tank is a positive pressure incubation tank; an incubation groove cover is arranged at the upper end of the positive pressure incubation groove body.
8. The molecular film washing apparatus according to claim 7, wherein a positive pressure gas pipe is provided on the positive pressure incubation tank; one end of the positive pressure air pipe is positioned below the incubation groove cover, and the other end of the positive pressure air pipe is connected with an air pump.
9. The molecular film washing device according to claim 8, wherein the incubation well cover is a sealing gel well cover and/or a part of a gel well cover.
10. A method of using the molecular film washing device according to any one of claims 1 to 9, comprising the steps of:
when the incubation groove body is a negative pressure incubation groove body, the waste liquid outlet is connected with a negative pressure pump, the waste liquid inlet is connected with the reagent groove, and the waste liquid pipe enables the part below the microporous filter plate to generate negative pressure through the negative pressure pump;
when the incubation groove body is a positive pressure incubation groove body, the positive pressure air pipe is connected with the air pump, the waste liquid outlet is connected with the waste liquid bottle and the negative pressure pump, the waste liquid inlet is connected with the reagent groove, and the positive pressure air pipe enables the space between the microporous filter plate and the incubation groove cover to form positive pressure through the air pump;
placing the membrane material on a microporous filter plate in an incubation groove body, adding a washing liquid, and then filtering out the washing liquid by suction;
taking out the film material after washing, adding a luminescent substrate or a chromogenic substrate, incubating for a period of time, and observing a target signal and a background signal by analyzing a gray value or visually comparing the two methods to obtain a developing result;
when waste liquid needs to be removed, the incubation groove body is inclined leftwards, so that the reagent in the incubation groove body flows out into a waste liquid collecting reagent bottle through a waste liquid outlet of a waste liquid pipe;
when the reagent needs to be recovered, the incubation groove body is inclined to the right, the reagent in the incubation groove body flows into the solution recovery groove, and the reagent is recovered in the solution recovery groove by using a pipette.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311156800.8A CN117180997A (en) | 2023-09-07 | 2023-09-07 | Molecular film washing device and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311156800.8A CN117180997A (en) | 2023-09-07 | 2023-09-07 | Molecular film washing device and application method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN117180997A true CN117180997A (en) | 2023-12-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311156800.8A Pending CN117180997A (en) | 2023-09-07 | 2023-09-07 | Molecular film washing device and application method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994019484A1 (en) * | 1993-02-18 | 1994-09-01 | Biocircuits Corporation | Disposable device in diagnostic assays |
| CN201744307U (en) * | 2010-05-13 | 2011-02-16 | 蓝十字生物药业（北京）有限公司 | Membrane cleaning device |
| CN109453670A (en) * | 2018-12-26 | 2019-03-12 | 上海禅睿实业有限公司 | A kind of electrodialysis membrane stack cleaning method |
| CN216350743U (en) * | 2021-11-23 | 2022-04-19 | 重庆斯成锐科技有限公司 | Full-automatic antibody hybridization instrument |
-
2023
- 2023-09-07 CN CN202311156800.8A patent/CN117180997A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994019484A1 (en) * | 1993-02-18 | 1994-09-01 | Biocircuits Corporation | Disposable device in diagnostic assays |
| CN201744307U (en) * | 2010-05-13 | 2011-02-16 | 蓝十字生物药业（北京）有限公司 | Membrane cleaning device |
| CN109453670A (en) * | 2018-12-26 | 2019-03-12 | 上海禅睿实业有限公司 | A kind of electrodialysis membrane stack cleaning method |
| CN216350743U (en) * | 2021-11-23 | 2022-04-19 | 重庆斯成锐科技有限公司 | Full-automatic antibody hybridization instrument |
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