CN117126258A - Application of pear oligopeptide PbeEP 4 in plant disease prevention and control - Google Patents
Application of pear oligopeptide PbeEP 4 in plant disease prevention and control Download PDFInfo
- Publication number
- CN117126258A CN117126258A CN202311097494.5A CN202311097494A CN117126258A CN 117126258 A CN117126258 A CN 117126258A CN 202311097494 A CN202311097494 A CN 202311097494A CN 117126258 A CN117126258 A CN 117126258A
- Authority
- CN
- China
- Prior art keywords
- pbeep
- short peptide
- pear
- control
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000014443 Pyrus communis Nutrition 0.000 title claims abstract description 31
- 230000006806 disease prevention Effects 0.000 title claims abstract description 13
- 102000015636 Oligopeptides Human genes 0.000 title claims abstract description 10
- 108010038807 Oligopeptides Proteins 0.000 title claims abstract description 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 82
- 241000196324 Embryophyta Species 0.000 claims abstract description 45
- 241000220324 Pyrus Species 0.000 claims abstract description 35
- 241000219109 Citrullus Species 0.000 claims abstract description 28
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 claims abstract description 26
- 241000221662 Sclerotinia Species 0.000 claims abstract description 20
- 230000036039 immunity Effects 0.000 claims abstract description 13
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 9
- 244000185180 Pyrus betulifolia Species 0.000 claims abstract description 8
- 235000006877 Pyrus betulifolia Nutrition 0.000 claims abstract description 8
- 230000002265 prevention Effects 0.000 claims abstract description 8
- 241000221696 Sclerotinia sclerotiorum Species 0.000 claims abstract description 6
- 244000052769 pathogen Species 0.000 claims description 15
- 238000011081 inoculation Methods 0.000 claims description 12
- 230000015572 biosynthetic process Effects 0.000 claims description 10
- 238000003786 synthesis reaction Methods 0.000 claims description 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 9
- 240000002791 Brassica napus Species 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 238000004458 analytical method Methods 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 235000011293 Brassica napus Nutrition 0.000 claims description 6
- 210000004899 c-terminal region Anatomy 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 5
- 241000123650 Botrytis cinerea Species 0.000 claims description 4
- 244000241235 Citrullus lanatus Species 0.000 claims description 4
- 235000009831 Citrullus lanatus Nutrition 0.000 claims description 4
- 241000588694 Erwinia amylovora Species 0.000 claims description 4
- 239000013604 expression vector Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 230000004044 response Effects 0.000 claims description 3
- 241000219104 Cucurbitaceae Species 0.000 claims description 2
- 241000220317 Rosa Species 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 2
- 238000010367 cloning Methods 0.000 claims description 2
- 230000028993 immune response Effects 0.000 claims description 2
- 208000013435 necrotic lesion Diseases 0.000 claims description 2
- 239000002773 nucleotide Substances 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 230000001902 propagating effect Effects 0.000 claims description 2
- 239000012752 auxiliary agent Substances 0.000 claims 1
- 230000000149 penetrating effect Effects 0.000 claims 1
- 238000009736 wetting Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 25
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 25
- 230000005284 excitation Effects 0.000 abstract description 13
- 238000005507 spraying Methods 0.000 abstract description 11
- 238000000034 method Methods 0.000 abstract description 9
- 208000035240 Disease Resistance Diseases 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 4
- 206010059866 Drug resistance Diseases 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 230000002194 synthesizing effect Effects 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 description 12
- PYIDGJJWBIBVIA-UYTYNIKBSA-N lauryl glucoside Chemical compound CCCCCCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PYIDGJJWBIBVIA-UYTYNIKBSA-N 0.000 description 11
- 229940048848 lauryl glucoside Drugs 0.000 description 11
- 230000003902 lesion Effects 0.000 description 11
- 239000007921 spray Substances 0.000 description 11
- 239000012154 double-distilled water Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 235000006008 Brassica napus var napus Nutrition 0.000 description 4
- 241000233614 Phytophthora Species 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 102000007863 pattern recognition receptors Human genes 0.000 description 4
- 108010089193 pattern recognition receptors Proteins 0.000 description 4
- 229920001184 polypeptide Polymers 0.000 description 4
- 244000088401 Pyrus pyrifolia Species 0.000 description 3
- 235000011400 Pyrus pyrifolia Nutrition 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 244000153158 Ammi visnaga Species 0.000 description 2
- 235000010585 Ammi visnaga Nutrition 0.000 description 2
- 241001465180 Botrytis Species 0.000 description 2
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 2
- 240000000385 Brassica napus var. napus Species 0.000 description 2
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- 241000195632 Dunaliella tertiolecta Species 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 235000018185 Betula X alpestris Nutrition 0.000 description 1
- 235000018212 Betula X uliginosa Nutrition 0.000 description 1
- 229920000018 Callose Polymers 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000195633 Dunaliella salina Species 0.000 description 1
- 244000141359 Malus pumila Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 241000233616 Phytophthora capsici Species 0.000 description 1
- 244000173166 Pyrus ussuriensis Species 0.000 description 1
- 235000011572 Pyrus ussuriensis Nutrition 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000004665 defense response Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 238000012809 post-inoculation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Biodiversity & Conservation Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Dentistry (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Agronomy & Crop Science (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention provides an application of pear oligopeptide PbeEP 4 in plant disease prevention and control, in particular to an application of pear (Pyrus betulifolia) oligopeptide PbeEP 4 in pear blight, rape sclerotinia and watermelon gray mold prevention and control. The invention firstly clarifies the excitation effect of the short peptide PbeEP 4 on the disease resistance by artificially synthesizing the short peptide PbeEP 4, and provides the method for preventing and controlling the disease by respectively exciting the resistance of pear, rape and watermelon to fire epidemic disease, sclerotinia sclerotiorum and gray mold by spraying. The PbeEP 4 short peptide provided by the invention is a product synthesized by plants, and the short peptide is utilized to excite immunity so as to prevent and treat diseases, and has the advantages of natural green, environment friendliness, no possibility of inducing pathogenic substances to generate drug resistance and the like. In addition, the short peptide is easy to synthesize artificially, has simple and convenient acquisition, strong disease resistance excitation effect and low required concentration, and is a novel ecological and environment-friendly crop disease prevention and control agent.
Description
Technical Field
The invention belongs to the technical field of plant immune biology, relates to application of pear oligopeptide PbeEP 4 in plant disease prevention and control, and relates to application of pear oligopeptide PbeEP 4 in immunity and disease resistance excitation function, in particular to application of pear oligopeptide PbeEP 4 in green prevention and control of various crop diseases such as pear fire blight, sclerotinia rot and watermelon gray mold.
Background
1. Plant immunity excitation technology
Plant immunity is a defense response activated by plants recognizing pathogen molecules through receptors. The plant immune system has multiple layers, where the first layer is the pattern recognition receptors (pattern recognition receptors, PRRs) on the surface of plant cells that recognize immunity from the pathogen and the plant's own conserved molecular patterns, called pattern-triggered immunity (PTI). Plants are able to sense the disruption of plant cell integrity caused by pathogen infection and the like and generate injury-related molecular patterns (damage-associated molecular patterns, DAMPs) or synthetic plant cytokines (phytotytokines), which stimulate plants to generate PTI immune responses through PRR receptor recognition, including active oxygen bursts, activation of kinase cascades, callose deposition, expression of defense-related genes, and the like, ultimately exhibiting resistance to pathogens. PTI plays an important role in preventing non-host resistance (nonhost resistance) of non-adaptive microorganisms to infest plants and limiting basal resistance (basal resistance) of adaptive pathogens to infest diseased host plants. The immunity stimulated by DAMPs/phytophytokines has the advantages of broad spectrum, strong, stable and durable property and the like. In addition, because DAMPs/phytocityokines are natural products of plants, the DAMPs/phytocityokines are utilized to excite immunity so as to achieve the purpose of preventing or controlling diseases, and the method is a novel environment-friendly green disease prevention and control method.
2. Plant disease prevention and control technology
Crop diseases generally cause 10-30% yield loss, and disease prevention and control are important guarantees of grain safety. The measures for preventing and controlling crop diseases comprise plant quarantine, breeding and utilizing disease-resistant varieties, agricultural control, biological control, physical control, chemical control and the like. The efficient green prevention and control and the broad-spectrum comprehensive prevention and control of various diseases are development trends of crop disease prevention and control. The pear fire epidemic disease, the rape sclerotinia rot and the watermelon gray mold are all important crop diseases in agricultural production. The fire epidemic disease is caused by the bacteria Erwinia amylovora (Erwinia amylovora), is a destructive disease of fruit trees such as pears, apples and the like, and is also an important quarantine disease in China. Sclerotinia sclerotiorum (Sclerotinia sclerotiorum) is a main disease of oil crops such as rape and the like and vegetable crops. Gray mold is caused by Botrytis cinerea and is an important disease of crops such as vegetables, melons, fruits and the like. These diseases cause a huge economic loss each year. Chemical control remains an important means of controlling these diseases. Because of the problems of ecological pollution, human and animal toxicity, easy occurrence of drug resistance of pathogens and the like of some pesticides, novel green broad-spectrum control preparations are urgently needed to be developed.
Disclosure of Invention
The invention aims to provide an application of pear (Pyrus betulifolia) short peptide PbeEP 4 in plant disease prevention and control, and the application of the short peptide PbeEP 4 in green prevention and control of various crop diseases such as pear fire blight, rape sclerotinia rot, watermelon gray mold and the like. The amino acid sequence of the pear short peptide PbeEP 4 applied by the invention is shown as SEQ ID:1 is the C-terminal part of the full-length protein, and the full-length protein sequence is shown as SEQ ID: 2. The invention relates to an application of pear short peptide PbeEP 4 in immunity and disease resistance excitation.
The invention utilizes the pear short peptide PbeEP 4 to excite the rose crop pyrus (Pyrus betulifolia) to generate resistance to fire blight (Erwinia amylovora), the cruciferous crop rape (Brassica napus) to generate resistance to sclerotinia (Sclerotinia sclerotiorum) and the cucurbitaceae crop watermelon (Citrullus lanatus) to generate resistance to gray mold (Botrytis cinerea), thereby greenly preventing and controlling pear fire blight, rape sclerotinia and watermelon gray mold.
Prior to the present invention, the function of the short peptide PbeEP 4 was not reported in any publication. The invention firstly clarifies the excitation effect of the short peptide on resistance to pear fire blight, sclerotinia rot of colza and gray mold of watermelon through artificially synthesizing the short peptide PbeEP 4, and provides the application of the short peptide in preventing and controlling important diseases of crops by respectively exciting the resistance to pear fire blight, sclerotinia rot and gray mold of colza and watermelon through treating the pear, the rape and the watermelon. The application of the invention is realized by the following steps:
(1) Artificial synthesis or biological expression acquisition of PbeEP 4 short peptide
Professional polypeptide synthesis companies can be commissioned for synthesis. The short peptide pbep 4 can also be obtained by biological expression: cloning the corresponding nucleotide sequence of PbeEP 4 into an expression vector, converting the expression vector into eukaryotic or prokaryotic microorganism, propagating the microorganism, extracting and purifying the target short peptide.
(2) Plant treatment of PbeEP 4 short peptides
Plant leaves were spray-treated with an aqueous solution of the short peptide PbeEP 4 (containing 0.1% lauryl glucoside) at the appropriate concentration to stimulate plant immunity. The spray treatment enables large scale plant treatment.
(3) PbeEP 4 short peptides stimulate plants to develop resistance to pathogens
The short peptide PbeEP 4 rapidly elicits immune and disease-resistant responses in plants to a variety of pathogens after treatment. The intensity of the excitation can be detected by pathogen inoculation analysis. The immune and disease-resistant response of plants to pathogens is manifested by a short peptide pbep 4 treatment that results in less necrotic lesions after inoculation than in the water control treatment.
The invention has the advantages that: (1) The PbeEP 4 short peptide provided by the invention is a product synthesized by plants, and the PbeEP 4 short peptide is utilized to excite immunity so as to prevent crop diseases, thus the PbeEP 4 short peptide has the advantages of natural green, no environmental pollution, no harm to people and livestock, no drug resistance of pathogenic matters, and the like. (2) The DAMP/phytocotine stimulated plant immunity is the latest theoretical research result. PbeEP 4 is used as a sufficient theoretical basis for applying DAMP/phytocine to crop immune excitation and disease prevention and control, and is also an example of theoretical guidance practice application. (3) The PbeEP 4 short peptide has only 28 amino acids, is easy to artificially synthesize, and is easy to obtain. (4) The PbeEP 4 short peptide has strong disease resistance excitation effect and low required concentration, so that the disease control cost is lower.
Drawings
Figure 1 shows that spraying the leaves of pyrus pyrifolia (Pyrus betulifolia) with an aqueous solution of pbep 4 short peptide elicits resistance to fire blight in pyrus pyrifolia. Spraying birch leaf with 1 μm PbeEP 4 short peptide aqueous solution (containing 0.1% laurylglucoside) at OD in advance after 1d 600 0.5 of Phytophthora fireThe toothpick soaked in the liquid for half an hour is penetrated into the needle eye of the main vein of the leaf formed by aseptic needle prick to inoculate the leaf, and the fresh-keeping bag is sleeved for moisture preservation and culture at the temperature of 27 ℃. The onset is shown as 10d after inoculation. The results show that compared with the aseptic double distilled water (containing 0.1% of lauryl glucoside) treatment (Mock), the area of the formed lesion of the PbeEP 4 treatment blade is obviously smaller, and the lesion expansion is severely inhibited. The PbeEP 4 treatment is shown to strongly excite the pyrus to develop resistance to phytophthora fire.
FIG. 2 shows that spraying canola (Brassica napus) leaves with an aqueous solution of PbeEP 4 short peptide stimulates canola resistance to sclerotinia. Spraying 1 μm PbeEP 4 short peptide water solution (containing 0.1% laurylglucoside) on rape leaves, inoculating the leaves with sclerotinia mycelium block after 1d, and performing film-covered moisturizing culture at 23 ℃. The lesion size is shown as 1d after inoculation. The results show that compared with the aseptic double distilled water (containing 0.1% of lauryl glucoside) treatment (Mock), the formed lesions of the PbeEP 4 treatment leaves are obviously smaller, and the spread of the lesions is severely inhibited. It was shown that PbeEP 4 treatment strongly stimulated resistance to Sclerotinia.
Fig. 3 shows that spraying the leaves of watermelon (Citrullus lanatus) with an aqueous solution of pbep 4 short peptide stimulates watermelon to develop resistance to gray mold. Watermelon leaves were sprayed with 1. Mu.M PbeEP 4 short peptide aqueous solution (containing 0.1% lauryl glucoside) at a concentration of 1X 10 per ml at 2.5. Mu.L per spot after 1d 5 The conidium suspension of each spore is inoculated into the leaf, each leaf is connected with four points, and the membrane is covered at 23 ℃ for moisture preservation culture. The onset is shown as 65h post inoculation. The results show that compared with the aseptic double distilled water (containing 0.1% of lauryl glucoside) treatment (Mock), the formed lesions of the PbeEP 4 treatment leaves are obviously smaller, and the spread of the lesions is severely inhibited. It was shown that the PbeEP 4 treatment strongly stimulated watermelon to develop resistance to gray mold.
Detailed Description
The invention is further described with reference to the drawings and examples.
Example 1 application of PbeEP 4 short peptide in fire blight resistance of Dunaliella salina (Pyrus betulifolia)
According to the embodiment of the invention, the artificially synthesized PbeEP 4 short peptide is utilized, and the pear is treated by a spraying method to excite the pear to generate resistance to fire epidemic pathogens, so that the pear is used for preventing and controlling pear fire epidemic diseases. The method mainly comprises the following steps:
1) Artificial synthesis of PbeEP 4 short peptide
The PbeEP 4 short peptide sequence provided by the invention is shown as SEQ ID:1, consisting of 28 amino acids in total, is the C-terminal portion of the full-length protein (sequence shown as SEQ ID: 2). Prior to the present invention, the function of the short peptide PbeEP 4 was not reported in any publication.
The embodiment entrusts professional polypeptide synthesis company (blaze biotechnology limited company) to synthesize PbeEP 4 short peptide for analysis and application of pear disease resistance excitation and fire disease prevention and control functions.
2) Treatment of birch-leaf pear with PbeEP 4 short peptide
The plant treatment mode of the PbeEP 4 short peptide is spraying. The leaves of the pyrus plants were sprayed with an aqueous solution of PbeEP 4 short peptide (containing 0.1% lauryl glucoside) at a concentration of 1. Mu.M, enabling large-scale treatment of the plants.
3) PbeEP 4 short peptide stimulates the pyrus pyrifolia to generate resistance to phytophthora capsici
Spraying the leaves of the Dunaliella tertiolecta with PbeEP 4 short peptide aqueous solution (containing 0.1% lauryl glucoside) can significantly excite the Dunaliella tertiolecta to generate resistance to phytophthora fire. The results of the fire epidemic inoculation analysis show that after 1 mu M PbeEP 4 short peptide spray treatment for 1d, the formed lesions of the leaves inoculated in a mode that the fire epidemic bacteria carry bacteria toothpicks to penetrate into main veins are remarkably smaller than those of a sterile double-distilled water treatment control after 10d inoculation, and the spread of the lesions is seriously inhibited (figure 1). The PbeEP 4 spray treatment can strongly excite the pyrus ussuriensis to generate resistance to phytophthora fire.
Example 2 application of PbeEP 4 short peptide in anti-sclerotinia of rape (Brassica napus)
According to the embodiment of the invention, the artificially synthesized PbeEP 4 short peptide is utilized to treat rape by a spray method, so that the rape is stimulated to generate resistance to sclerotinia sclerotiorum, and the method is used for controlling sclerotinia rot of rape. The method mainly comprises the following steps:
1) Artificial synthesis of PbeEP 4 short peptide
The PbeEP 4 short peptide sequence provided by the invention is shown as SEQ ID:1, consisting of 28 amino acids in total, is the C-terminal portion of the full-length protein (sequence shown as SEQ ID: 2). Prior to the present invention, the function of the short peptide PbeEP 4 was not reported in any publication.
The embodiment entrusts professional polypeptide synthesis company (blaze biotechnology limited company) to synthesize PbeEP 4 short peptide for analyzing and applying rape disease resistance excitation and sclerotinia prevention and control functions.
2) Treatment of rape with PbeEP 4 short peptide
The plant treatment mode of the PbeEP 4 short peptide is spraying. The rape plant leaves are sprayed with a 1 μm concentration of an aqueous solution of PbeEP 4 short peptide (containing 0.1% lauryl glucoside) to enable large-scale treatment of plants.
3) PbeEP 4 short peptide stimulates rape to develop resistance to sclerotinia
The PbeEP 4 short peptide aqueous solution sprayed on rape leaves can obviously stimulate the rape to generate resistance to sclerotinia. The analysis results of the sclerotinia inoculation show that after 1d of 1 mu M PbeEP 4 short peptide spray treatment, the lesion spread of the leaves inoculated with the sclerotinia mycelium block after 1d of inoculation is significantly smaller than that of a sterile double distilled water treatment control (figure 2). The PbeEP 4 spray treatment can rapidly and strongly excite the rape to generate the resistance to sclerotinia.
Example 3 application of PbeEP 4 short peptide in resisting gray mold of watermelon (Citrullus lanatus)
According to the embodiment of the invention, the artificially synthesized PbeEP 4 short peptide is utilized, and the watermelon is treated by a spray method, so that the watermelon is stimulated to generate the resistance to gray mold bacteria, thereby being used for controlling the gray mold of the watermelon. The method mainly comprises the following steps:
1) Artificial synthesis of PbeEP 4 short peptide
The PbeEP 4 short peptide sequence provided by the invention is shown as SEQ ID:1, consisting of 28 amino acids in total, is the C-terminal portion of the full-length protein (sequence shown as SEQ ID: 2). Prior to the present invention, the function of the short peptide PbeEP 4 was not reported in any publication.
The embodiment entrusts professional polypeptide synthesis company (blaze biotechnology limited company) to synthesize PbeEP 4 short peptide for analysis and application of disease resistance excitation and gray mold prevention and control functions of watermelons.
2) Treatment of watermelon with PbeEP 4 short peptide
The plant treatment mode of the PbeEP 4 short peptide is spraying. The watermelon plant leaves were sprayed with 1 μm concentration of pbep 4 short peptide aqueous solution (containing 0.1% lauryl glucoside) to enable large scale treatment of plants.
3) PbeEP 4 short peptide excitation watermelon to generate resistance to botrytis cinerea
The PbeEP 4 short peptide aqueous solution spray watermelon leaves can obviously excite watermelon to generate resistance to gray mold bacteria. The results of the botrytis inoculation analysis show that after 1 mu M PbeEP 4 short peptide spray treatment for 1d, the botrytis is treated with a conidium suspension (concentration of 1X 10 per ml 5 Individual spores) were inoculated at 2.5 μl per spot and lesions spread were significantly smaller than the sterile double distilled water control 65h after inoculation (fig. 3). The PbeEP 4 spray treatment is shown to strongly excite the watermelon to generate resistance to the gray mold bacteria.
Claims (3)
1. The application of the pear oligopeptide PbeEP 4 in plant disease prevention and control is characterized in that the pear (Pyrus betulifolia) oligopeptide PbeEP 4 is applied to pear fire blight, rape sclerotinia and watermelon gray mold prevention and control, and the amino acid sequence of the pear oligopeptide PbeEP 4 is shown as SEQ ID:1 is the C-terminal part of the full-length protein, and the full-length protein sequence is shown as SEQ ID: 2.
2. The use according to claim 1, characterized in that the pear short peptide pbep 4 is used to stimulate the rose crop pyrus (Pyrus betulifolia) to produce resistance to fire blight (Erwinia amylovora), the cruciferous crop Brassica napus (Brassica napus) to sclerotinia (Sclerotinia sclerotiorum) and the cucurbitaceae crop citrullus (Citrullus lanatus) to gray mold (Botrytis cinerea), thereby green control of pear fire blight, brassica napus and watermelon gray mold.
3. The use according to claim 1, characterized by the following steps:
(1) Artificial synthesis or biological expression acquisition of PbeEP 4 short peptide
Obtaining a short peptide PbeEP 4: cloning the corresponding nucleotide sequence of PbeEP 4 into an expression vector, converting the expression vector into eukaryotic or prokaryotic microorganisms, propagating the microorganisms, extracting and purifying target short peptides;
(2) Plant treatment of PbeEP 4 short peptides
The plant leaves are sprayed with the short peptide PbeEP 4 water solution containing wetting and penetrating auxiliary agents to excite plant immunity, so that the plants can be treated on a large scale;
(3) PbeEP 4 short peptides stimulate plants to develop resistance to pathogens
The short peptide PbeEP 4 treatment elicits an immune and resistant response to the pathogen in the plant, and the intensity of the elicitation is detected by pathogen inoculation analysis, and the immune response to the pathogen in the plant is expressed as a short peptide PbeEP 4 treatment, which results in less necrotic lesions after inoculation than in the water control treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311097494.5A CN117126258A (en) | 2023-08-29 | 2023-08-29 | Application of pear oligopeptide PbeEP 4 in plant disease prevention and control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311097494.5A CN117126258A (en) | 2023-08-29 | 2023-08-29 | Application of pear oligopeptide PbeEP 4 in plant disease prevention and control |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117126258A true CN117126258A (en) | 2023-11-28 |
Family
ID=88852352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311097494.5A Pending CN117126258A (en) | 2023-08-29 | 2023-08-29 | Application of pear oligopeptide PbeEP 4 in plant disease prevention and control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117126258A (en) |
-
2023
- 2023-08-29 CN CN202311097494.5A patent/CN117126258A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Woo et al. | Exploiting the interactions between fungal antagonists, pathogens and the plant for biocontrol | |
| Boyette et al. | Biological control of kudzu (Pueraria lobata) with an isolate of Myrothecium verrucaria | |
| Khmel et al. | Biological control of crown gall in grapevine and raspberry by two Pseudomonas spp. with a wide spectrum of antagonistic activity | |
| Narayanasamy et al. | Mechanisms of action of fungal biological control agents | |
| CN110278959B (en) | Application of rape short peptide BnPEP4 | |
| Upadhyay et al. | Biocontrol agents of plant pathogens: their use and practical constraints | |
| Sunpapao et al. | Effects of chitosan treatments on para rubber leaf fall disease caused by Phytophthora palmivora Butler-a laboratory study. | |
| Cook | Plant health and the sustainability of agriculture, with special reference to disease control by beneficial microorganisms | |
| CN106350459B (en) | Produce volatile antibacterial, eelworm-killing activity ingredient schizophyllum commune bacterial strain and its application | |
| Nathaniels et al. | Latent infection of winter oilseed rape by Leptosphaeria maculans | |
| CN110301454B (en) | Application of rape short peptide BnPEP5 | |
| Cruz-Martín et al. | Antifungal activity of Bacillus amyloliquefaciens against Fusarium oxysporum F. sp. cubense race 1. | |
| CN114375963B (en) | Immune excitation function application of rape short peptide BnPEP6 | |
| CN116849228B (en) | Application of pear oligopeptide PdrPEP6 in plant disease prevention and control | |
| CN117126258A (en) | Application of pear oligopeptide PbeEP 4 in plant disease prevention and control | |
| Mona et al. | Seed bio priming as biological approach for controlling root rot soil born fungi on soybean (Glycine max L.) plant | |
| CN113201059A (en) | Dichocrocis punctiferalis active antibacterial peptide, gene, recombinant vector and application thereof | |
| Chen et al. | Cultivar-specific induction of necrosis by exudates from basidiospore germlings of the cowpea rust fungus | |
| JPH06340696A (en) | Antibacterial protein | |
| Varma | Prevalence of Leptotrombidium deliense, the scrub typhus vector, in the eastern Himalayas | |
| CN106818885A (en) | A kind of acid protease is preparing the application in suppressing cause of disease bacteria preparation | |
| Tjamos | Induction of resistance to Verticillium wilt in cucumber (Cucumis sativus) | |
| KR20030015163A (en) | New biopesticide using gene from Erwinia pyrifoliae WT#3, novel pathogen that affects Asian pear trees | |
| Van Rooi et al. | Fungicide efficacy against Botrytis cinerea at different positions on grape shoots | |
| El‐Goorani et al. | The effect of Bacillus subtilis on in vitro growth and pathogenicity of Erwinia amylovora |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |