CN117110195A - Biomembrane adhesion detection device and detection method - Google Patents
Biomembrane adhesion detection device and detection method Download PDFInfo
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- CN117110195A CN117110195A CN202311214112.2A CN202311214112A CN117110195A CN 117110195 A CN117110195 A CN 117110195A CN 202311214112 A CN202311214112 A CN 202311214112A CN 117110195 A CN117110195 A CN 117110195A
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- 238000001514 detection method Methods 0.000 title claims abstract description 165
- 239000007788 liquid Substances 0.000 claims abstract description 75
- 239000012528 membrane Substances 0.000 claims abstract description 26
- 238000007789 sealing Methods 0.000 claims abstract description 5
- 238000012360 testing method Methods 0.000 claims description 23
- 239000011521 glass Substances 0.000 claims description 6
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims description 3
- 230000001678 irradiating effect Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims 3
- 239000012620 biological material Substances 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 210000001951 dura mater Anatomy 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000002706 hydrostatic effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000005201 scrubbing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N19/00—Investigating materials by mechanical methods
- G01N19/04—Measuring adhesive force between materials, e.g. of sealing tape, of coating
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01M—TESTING STATIC OR DYNAMIC BALANCE OF MACHINES OR STRUCTURES; TESTING OF STRUCTURES OR APPARATUS, NOT OTHERWISE PROVIDED FOR
- G01M3/00—Investigating fluid-tightness of structures
- G01M3/02—Investigating fluid-tightness of structures by using fluid or vacuum
- G01M3/04—Investigating fluid-tightness of structures by using fluid or vacuum by detecting the presence of fluid at the leakage point
- G01M3/20—Investigating fluid-tightness of structures by using fluid or vacuum by detecting the presence of fluid at the leakage point using special tracer materials, e.g. dye, fluorescent material, radioactive material
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the technical field of biological material detection, in particular to a biological film adhesion force detection device and a detection method, wherein the biological film adhesion force detection device comprises: the device comprises a detection box, wherein the top end of the detection box is fixedly connected with a detection film, an opening is formed in the detection film, a biological membrane is adhered to the opening of the detection film, the top end of the detection box is fixedly connected with a first scale tube, one side of the detection box is fixedly inserted with a detection tube structure, the detection tube structure comprises a flat tube, one end of the flat tube is fixedly provided with a connector, a sealing backing ring is sleeved on the connector, detection liquid is injected into the detection tube structure, one side of the detection box is fixedly connected with a detection lamp structure, and the detection lamp structure is matched with the detection liquid; the invention is provided with the first scale tube, so that the influence of residual air in the detection box on the detection structure is avoided, and the second scale tube is provided, so that the influence of external air entering the detection box through the detection tube structure on the detection result is avoided when liquid is fed.
Description
Technical Field
The invention belongs to the technical field of biological material detection, and particularly relates to a biological film adhesion force detection device and a biological film adhesion force detection method.
Background
A dural (spinal) membrane patch refers to a sheet-like material product for temporary or permanent repair of dura mater and/or dura mater defects, which requires testing for maximum hydrostatic pressure it can withstand and the time to remain leak-free at such pressure to determine its barrier properties.
Chinese patent publication No. CN111189775a discloses a biofilm adhesion force detecting device and method, which uses a U-tube to measure the adhesion force change between a biofilm to be detected and a test film, thereby rapidly calculating the adhesion force.
However, when the liquid level in the test vessel is higher than the interface of the U-shaped pipe on the side wall during the test, the residual air in the test vessel cannot be discharged, so that a certain influence can be caused on the test result.
Disclosure of Invention
Aiming at the problem that the residual air in the test vessel cannot be discharged and can cause certain influence on the test result in the prior art, the invention provides the following technical scheme:
a biofilm adhesion force detection device comprising: the detecting box, the top fixedly connected with test membrane of detecting box is equipped with the opening on the test membrane, and the opening part of test membrane bonds there is the biomembrane, and the top fixedly connected with of detecting box has first graduated flask, and the fixed grafting in one side of detecting box has the detecting tube structure, has the detection liquid in the detecting tube structure, and the one side fixedly connected with of detecting box detects the lamp structure, detects the lamp structure and detects the liquid phase and match.
Preferably, the detection box is opaque, and the first scale tube is an open glass tube.
As the optimization of above-mentioned technical scheme, detect the pipe structure and include the flat pipe, the fixed joint that is equipped with of one end of flat pipe has cup jointed sealed backing ring on the joint, and flat pipe passes through to connect and sealed backing ring and the fixed grafting of detection box.
As the optimization of the technical scheme, the other end of the flat pipe is fixedly connected with a liquid inlet hose, and one end of the liquid inlet hose is fixedly connected with an external liquid inlet structure.
As the optimization of the technical scheme, the middle part of the top end of the flat tube is fixedly provided with a second scale tube, the second scale tube is an open glass tube, and the height of the second scale tube is matched with that of the first scale tube.
As the preferable choice of the technical proposal, the detection liquid is cerebrospinal fluid analog liquid, and the detection liquid is doped with fluorescent leak detection agent.
As the preference of above-mentioned technical scheme, detect lamp structure includes the lamp stand, and one side and the detection box fixed connection of lamp stand, the top of lamp stand articulates there is the mounting panel, and the bottom of mounting panel articulates there is the lamp shade, installs the lamp area in the lamp shade, and the light that the lamp area sent is blue light or ultraviolet light.
As a preferable mode of the above technical solution, an external camera is placed beside the detection box, and the shooting range of the external camera includes the test film.
The detection method of the biological film adhesion detection device comprises the following steps:
s1: extending and opening the detection lamp structure, irradiating the detection box and the detection tube structure, and simultaneously conveying detection liquid into the flat tube from the liquid inlet hose by utilizing the external liquid inlet structure;
s2: the detection liquid flows into the detection box through the joint, the liquid level in the detection box gradually rises, and gas in the detection box escapes from the first scale tube during the period;
s3: when the detection box is filled with detection liquid, the detection liquid enters the first scale tube, and after the liquid in the first scale tube rises to a certain height, the hydraulic pressure of the detection liquid exceeds the adhesion force of the biological membrane;
s4: the detection liquid leaks from the bonding part of the biological membrane and the test membrane, and under the irradiation of the detection lamp structure, a trace is displayed on the surface of the detection box and is captured by an external camera;
s5: under the irradiation of the detection lamp structure, in the picture shot by the observation camera, when the detection liquid leaks, the marks left by the detection liquid on the first scale tube and the second scale tube are detected, and then the adhesion force of the biological membrane is calculated.
The beneficial effects of the invention are as follows:
(1) The invention is provided with the first scale tube, so that the influence of residual air in the detection box on the detection structure during detection is avoided, and the second scale tube is provided, so that the influence of external air entering the detection box through the detection tube structure during liquid inlet on the detection result is avoided;
(2) The invention uses the fluorescence leak detection method, utilizes an external camera to capture the leakage trace of the detection liquid, has no dead angle compared with the capture of a sensor, and has no problem of influencing the detection result due to improper installation of the sensor.
Drawings
FIG. 1 is a schematic view showing the overall structure of the present invention;
FIG. 2 is a schematic view showing the structure of a detection tube according to the present invention;
fig. 3 shows a schematic diagram of the structure of the inspection lamp of the present invention.
In the figure: 1. a detection box; 2. testing the film; 3. a biological membrane; 4. a first scale tube; 5. a detection tube structure; 501. a flat tube; 502. a joint; 503. a seal grommet; 504. a liquid inlet hose; 505. a second scale tube; 6. detecting a lamp structure; 601. a lamp holder; 602. a support plate; 603. a lampshade.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the embodiments.
Example 1
A biofilm adhesion force detecting device, as shown in fig. 1 to 3, comprising: the detection box 1, the top fixedly connected with test membrane 2 of detection box 1, be equipped with the opening on the test membrane 2, the opening part of test membrane 2 bonds and has biological membrane 3, the top fixedly connected with first graduated flask 4 of detection box 1, the fixed grafting of one side of detection box 1 has detection tube structure 5, has the detection liquid in the detection tube structure 5, the one side fixedly connected with detection lamp structure 6 of detection box 1, detection lamp structure 6 and detection liquid phase match.
The invention uses a fluorescence leak detection method, the principle is that whether detection liquid flows onto a detection box 1 through a gap of a biological membrane 3 is observed by utilizing the principle that a fluorescence leak detection agent emits bright yellow-green light under ultraviolet/blue light leak detection irradiation, so as to determine whether the detection liquid leaks and calculate the adhesion of the biological membrane 3.
Referring to fig. 1 of the specification, a detection box 1 is opaque, so that detection liquid leakage marks can be observed conveniently, and a first scale tube 4 is an open glass tube, so that detection liquid marks can be observed conveniently.
The top end of the detection box 1 can be inclined upwards towards the direction of the first scale tube 4, so that air can better escape through the first scale tube 4.
Referring to fig. 1 and 2 of the specification, the detecting tube structure 5 includes a flat tube 501, one end of the flat tube 501 is fixedly provided with a joint 502, a sealing gasket 503 is sleeved on the joint 502, and the flat tube 501 is fixedly spliced with the detecting box 1 through the joint 502 and the sealing gasket 503.
The detection tube structure 5 and the detection box 1 are of a split structure, and are convenient to store when not in use.
Referring to fig. 1 and 2 of the specification, the other end of the flat tube 501 is fixedly connected with a liquid inlet hose 504, one end of the liquid inlet hose 504 is fixedly connected with an external liquid inlet structure, and the external liquid inlet structure is a piston structure, and can be in a needle tube type or a glue machine type.
Referring to fig. 1 and 2 of the specification, a second scale tube 505 is fixedly arranged in the middle of the top end of the flat tube 501, the second scale tube 505 is an open glass tube, the height of the second scale tube 505 is matched with the height of the first scale tube 4, so that air entering through an external liquid inlet structure escapes from the second scale tube 505, and the second scale tube 505 can be compared with the first scale tube 4 for reference.
Referring to the attached figure 1 of the specification, the detection liquid is cerebrospinal fluid analog liquid, and the detection liquid is doped with fluorescent leak detection agent, so that bright yellow-green light can be emitted under ultraviolet/blue light leak detection irradiation.
Referring to fig. 1 and 3 of the specification, a detection lamp structure 6 includes a lamp holder 601, one side of the lamp holder 601 is fixedly connected with a detection box 1, a support plate 602 is hinged to the top end of the lamp holder 601, a lampshade 603 is hinged to the bottom end of the support plate 602, a lamp band is installed in the lampshade 603, and light emitted by the lamp band is blue light or ultraviolet light.
Referring to the attached drawing 1 of the specification, an external camera is placed beside the detection box 1, the shooting range of the external camera comprises a test film 2, the leakage trace of detection liquid is conveniently and accurately captured, and an external control structure can be added to link the external camera with an external liquid inlet structure, so that when the leakage trace of detection liquid is captured by the external camera, the liquid inlet structure immediately stops liquid inlet, the speed is faster than that of manual operation, and the leakage trace on the detection box 1 is smaller, so that the subsequent scrubbing is convenient.
The detection method of the biological film adhesion detection device comprises the following steps:
s1: extending and turning on the detection lamp structure 6 to illuminate the detection cartridge 1 and the detection tube structure 5 while feeding the detection liquid from the liquid feeding hose 504 into the flat tube 501 by using the external liquid feeding structure;
s2: the detection liquid flows into the detection box 1 through the joint 502, the liquid level in the detection box 1 gradually rises, and the gas in the detection box 1 escapes from the first scale tube 4;
s3: when the detection box 1 is filled with detection liquid, the detection liquid enters the first scale tube 4, and after the liquid in the first scale tube 4 rises to a certain height, the hydraulic pressure of the detection liquid exceeds the adhesive force of the biological membrane 3;
s4: the detection liquid leaks from the bonding position of the biological membrane 3 and the test membrane 2, and under the irradiation of the detection lamp structure 6, the trace is exposed on the surface of the detection box 1 and is captured by an external camera;
s5: when the detection lamp structure 6 irradiates, and the image shot by the camera is observed, and the detection liquid leaks, the marks left by the detection liquid on the first scale tube 4 and the second scale tube 505 are detected, and then the adhesion of the biological membrane 3 is calculated.
Although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (8)
1. A biofilm adhesion force detection device, characterized by comprising: the detection device comprises a detection box (1), wherein the top end of the detection box (1) is fixedly connected with a detection film (2), an opening is formed in the detection film (2), a biological membrane (3) is adhered to the opening of the detection film (2), a first scale tube (4) is fixedly connected to the top end of the detection box (1), a detection tube structure (5) is fixedly inserted into one side of the detection box (1), detection liquid is injected into the detection tube structure (5), a detection lamp structure (6) is fixedly connected to one side of the detection box (1), and the detection lamp structure (6) is matched with a detection liquid;
the detecting tube structure (5) comprises a flat tube (501), a connector (502) is fixedly arranged at one end of the flat tube (501), a sealing backing ring (503) is sleeved on the connector (502), and the flat tube (501) is fixedly spliced with the detecting box (1) through the connector (502) and the sealing backing ring (503).
2. A biofilm adhesion force detecting device according to claim 1, characterized in that said cartridge (1) is opaque and said first scale tube (4) is an open glass tube.
3. The biomembrane adhesion force detection device according to claim 1, wherein the other end of the flat tube (501) is fixedly connected with a liquid inlet hose (504), and one end of the liquid inlet hose (504) is fixedly connected with an external liquid inlet structure.
4. A biofilm adhesion force detecting device according to claim 3, characterized in that a second scale tube (505) is fixedly arranged in the middle of the top end of the flat tube (501), the second scale tube (505) is an open glass tube, and the height of the second scale tube (505) is matched with the height of the first scale tube (4).
5. The device of claim 1, wherein the test fluid is cerebrospinal fluid analog fluid, and wherein the test fluid is doped with a fluorescent leak detector.
6. The biomembrane adhesion detection device according to claim 1, characterized in that the detection lamp structure (6) comprises a lamp holder (601), one side of the lamp holder (601) is fixedly connected with the detection box (1), a support plate (602) is hinged to the top end of the lamp holder (601), a lamp cover (603) is hinged to the bottom end of the support plate (602), a lamp strip is installed in the lamp cover (603), and light emitted by the lamp strip is blue light or ultraviolet light.
7. A biofilm adhesion force detecting device according to claim 1, characterized in that an external camera is placed beside the detection box (1), and the shooting range of the external camera comprises the test membrane (2).
8. A method of detecting a biofilm adhesion detecting device according to any of claims 1 to 7, comprising the steps of:
s1: extending and turning on the detection lamp structure (6), irradiating the detection box (1) and the detection tube structure (5), and simultaneously conveying the detection liquid from the liquid inlet hose (504) into the flat tube (501) by utilizing the external liquid inlet structure;
s2: the detection liquid flows into the detection box (1) through the joint (502), the liquid level in the detection box (1) is gradually increased, and the gas in the detection box (1) escapes from the first scale tube (4);
s3: when the detection box (1) is filled with detection liquid, the detection liquid enters the first scale tube (4) along with the detection liquid, and after the liquid in the first scale tube (4) rises to a certain height, the liquid pressure of the detection liquid exceeds the adhesion force of the biological membrane (3);
s4: the detection liquid leaks from the bonding part of the biological membrane (3) and the test membrane (2), and under the irradiation of the detection lamp structure (6), trace is displayed on the surface of the detection box (1) and captured by an external camera;
s5: under the irradiation of the detection lamp structure (6), observing marks left by detection liquid on the first scale tube (4) and the second scale tube (505) when the detection liquid leaks in the picture shot by the camera, and then calculating the adhesion of the biological membrane (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311214112.2A CN117110195A (en) | 2023-09-20 | 2023-09-20 | Biomembrane adhesion detection device and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311214112.2A CN117110195A (en) | 2023-09-20 | 2023-09-20 | Biomembrane adhesion detection device and detection method |
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| Publication Number | Publication Date |
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| CN117110195A true CN117110195A (en) | 2023-11-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202311214112.2A Pending CN117110195A (en) | 2023-09-20 | 2023-09-20 | Biomembrane adhesion detection device and detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111189775A (en) * | 2020-02-17 | 2020-05-22 | 冠昊生物科技股份有限公司 | Biomembrane adhesion detection device and detection method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150059488A1 (en) * | 2013-09-04 | 2015-03-05 | St. John's University | Film Adhesion Detection Device and Method Thereof |
| CN105675223A (en) * | 2016-01-18 | 2016-06-15 | 伟创力制造（珠海）有限公司 | Fluorescence leak detection method |
| CN207140403U (en) * | 2017-05-18 | 2018-03-27 | 中铁七局集团有限公司海外公司 | Tubeless type tire priming device |
| CN111189775A (en) * | 2020-02-17 | 2020-05-22 | 冠昊生物科技股份有限公司 | Biomembrane adhesion detection device and detection method |
| CN214040531U (en) * | 2020-11-22 | 2021-08-24 | 上海神力科技有限公司 | Fixed-point leak detection system for fuel cell polar plate |
-
2023
- 2023-09-20 CN CN202311214112.2A patent/CN117110195A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150059488A1 (en) * | 2013-09-04 | 2015-03-05 | St. John's University | Film Adhesion Detection Device and Method Thereof |
| CN105675223A (en) * | 2016-01-18 | 2016-06-15 | 伟创力制造（珠海）有限公司 | Fluorescence leak detection method |
| CN207140403U (en) * | 2017-05-18 | 2018-03-27 | 中铁七局集团有限公司海外公司 | Tubeless type tire priming device |
| CN111189775A (en) * | 2020-02-17 | 2020-05-22 | 冠昊生物科技股份有限公司 | Biomembrane adhesion detection device and detection method |
| CN214040531U (en) * | 2020-11-22 | 2021-08-24 | 上海神力科技有限公司 | Fixed-point leak detection system for fuel cell polar plate |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111189775A (en) * | 2020-02-17 | 2020-05-22 | 冠昊生物科技股份有限公司 | Biomembrane adhesion detection device and detection method |
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