CN117054384A - Fluorescence detection system - Google Patents
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- CN117054384A CN117054384A CN202311019468.0A CN202311019468A CN117054384A CN 117054384 A CN117054384 A CN 117054384A CN 202311019468 A CN202311019468 A CN 202311019468A CN 117054384 A CN117054384 A CN 117054384A
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- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 40
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- 238000005259 measurement Methods 0.000 description 10
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
Abstract
The application provides a fluorescence detection system for detecting weak fluorescence signals, which comprises: the atomic beam generating device is used for emitting atomic beam; the closed vacuum cavity is arranged at one side of the atomic beam emission device, the atomic beam is excited in the closed vacuum cavity to generate fluorescence, and the fluorescence is randomly emitted to the inner wall of the closed vacuum cavity; the reflecting structure and the photoelectric detection device are respectively positioned at the bottom and the top opposite to each other of the closed vacuum cavity in the direction perpendicular to the atomic beam current; the reflection structure is used for reflecting the fluorescence emitted to the bottom of the closed vacuum cavity, so that the fluorescence is emitted to the top of the vacuum cavity again and is received by the photoelectric detection device. The fluorescence detection system reduces the difficulty of collecting fluorescence signals generated by atomic transition and greatly improves the fluorescence collection rate.
Description
Technical Field
The application belongs to the field of atomic physics and precision measurement, and particularly relates to a fluorescence detection system for detecting weak fluorescence signals.
Background
The technology for realizing the research and precise measurement of the physical characteristics of the atoms by controlling the change of the quantum states of the atoms can be applied to a plurality of fields such as quantum communication, atomic clocks, atomic gravimeters, quantum simulation and the like. The atomic precision measurement result is expressed in the form of atomic quantum state distribution, and can be obtained by exciting atoms by specific laser to generate fluorescent radiation and detecting the intensity of the fluorescent radiation.
The above atomic transition generates a fluorescence signal, and in the process of detecting the fluorescence signal, the fluorescence signal needs to be collected first, however, the process of collecting the fluorescence signal by using the prior art generally has the following problems:
1. fluorescence random emission generated by atomic transition is distributed in the whole space angle;
2. because the fluorescence is distributed at the full space angle, when the cavity where the fluorescence is located is large, the fluorescence is very dispersed in the cavity, and the space solid angle corresponding to the detector is small, so that the collection of fluorescence signals is difficult, and the collection efficiency is sharply reduced;
3. based on the problem that the fluorescence signal is not easy to collect, the collection is performed by using a plurality of detectors in the prior art, so that the cost is high, and noise generated by the detectors is further enhanced, so that weak signal detection is not facilitated; in addition, higher demands are placed on the uniformity of the response times of the multiple detectors.
How to increase the collection proportion of weak fluorescence and increase the signal to noise ratio of fluorescence detection, thereby directly increasing the precision of quantum precise measurement is the subject to be studied at present.
Disclosure of Invention
According to the technical problem, the application provides the fluorescence collection system suitable for weak fluorescence signal detection, and the reflection structure and the detection structure are respectively arranged on the opposite sides of the vacuum cavity where the fluorescence signal is located, so that the fluorescence collection proportion of atomic groups or atomic beams is improved, and the signal to noise ratio of quantum precise measurement detection is further improved.
The present application provides a fluorescence detection system comprising: the atomic beam generating device is used for emitting atomic beam; the closed vacuum cavity is arranged at one side of the atomic beam emission device, the atomic beam is excited in the closed vacuum cavity to generate fluorescence, and the fluorescence is randomly emitted to the inner wall of the closed vacuum cavity; the reflecting structure and the photoelectric detection device are respectively positioned at the bottom and the top opposite to each other of the closed vacuum cavity in the direction perpendicular to the atomic beam current; the reflection structure is used for reflecting the fluorescence emitted to the bottom of the closed vacuum cavity, so that the fluorescence is emitted to the top of the vacuum cavity again and is received by the photoelectric detection device.
In some embodiments, the reflective structure is a reflective coating that covers the outside of the cavity wall at the bottom of the closed vacuum cavity, outside of the closed vacuum cavity.
In some embodiments, the reflecting structure is a reflecting prism structure, and a flat side of the reflecting prism structure is fixedly connected to the outer side of the cavity wall at the bottom of the closed vacuum cavity and is positioned at the outer side of the closed vacuum cavity; the reflecting prism structure comprises a single pyramid prism or a pyramid prism array formed by a plurality of miniature pyramid prisms.
In some embodiments, the reflecting structure is a concave mirror, and the distance between the atomic beam and the concave mirror is 1-2 times the focal length of the concave mirror.
In some embodiments, the reflecting structure is a combined reflecting structure of a second convex lens and a plane mirror, the second convex lens is located between the outer side of the bottom of the closed vacuum chamber and the reflecting plane of the plane mirror, and the distance between the second convex lens and the atomic beam is equal to the focal length of the second convex lens; and fluorescence emitted to the bottom of the closed vacuum cavity is converged by the second convex lens, continuously emitted to the reflection plane, reflected by the reflection plane, then emitted upwards by the second convex lens, emitted from the top of the closed vacuum cavity and received by the photoelectric detection device.
In some embodiments, the fluorescent light emitted from the top of the closed vacuum cavity is collected by the convex lens and then received by the photoelectric detection device; the distance between the first convex lens and the atomic beam current is 1-2 times of the focal length of the first convex lens.
In some embodiments, the distance between the first convex lens and the photodetection device is equal to or less than twice the focal length of the first convex lens.
In some embodiments, the photodetection device is attached to the outside of the cavity wall at the top of the closed vacuum cavity.
In some embodiments, the photodetection device completely covers the outside of the cavity wall at the top of the closed vacuum cavity.
In some embodiments, the opposing top and bottom chamber walls of the closed vacuum chamber are configured to be transparent.
Compared with the prior art, the fluorescent detection system provided by the application has the advantages that the reflection structures and the photoelectric detection devices which are positioned on two opposite sides of the atomic beam are arranged in the vertical direction of the atomic beam, fluorescent light emitted to the bottom cavity wall of the closed vacuum cavity is restrained to be emitted to the top cavity wall of the closed vacuum cavity again through the reflection structures, and the fluorescent light emitted to the top of the closed vacuum cavity together with fluorescent light emitted to the top cavity wall of the closed vacuum cavity is emitted to the photoelectric detection device, so that the fluorescent signal collection difficulty generated by atomic transition is reduced, and the fluorescent light collection rate is greatly improved. Meanwhile, as only one photoelectric detector is used, the requirements on the photoelectric detector are reduced, the cost is also reduced, and the fluorescence collection efficiency can be improved by 50-100%. In addition, a lens system (concave lens and convex lens) is added on the light path of fluorescence emitted from the top or bottom of the closed vacuum cavity, so that the fluorescence is converged, the requirement on the light-transmitting aperture of the photoelectric detector is reduced, and the noise generated by the photoelectric detector is greatly reduced.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings may be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a side view of a fluorescence detection system according to a first embodiment of the present application.
Fig. 2 is a side view of a fluorescence detection system according to a second embodiment of the present application.
Fig. 3 is a side view of a fluorescence detection system according to a third embodiment of the present application.
Fig. 4 is a side view of a fluorescence detection system according to a fourth embodiment of the present application.
Fig. 5 is a side view of a fluorescence detection system according to a fifth embodiment of the present application.
Fig. 6 is a side view of a fluorescence detection system according to a sixth embodiment of the present application.
FIG. 7 is a schematic diagram of the fluorescence reflection of a fluorescence detection system of the present application including pyramid corners.
Description of the embodiments
For a further understanding of the objects, construction, features, and functions of the application, reference should be made to the following detailed description of the preferred embodiments.
In the description of the present application, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application.
The application aims to provide a fluorescence detection system suitable for weak fluorescence detection, wherein a reflection structure and a photoelectric detection device are respectively arranged in the direction perpendicular to atomic beam current, so that unordered and divergent fluorescence generated by excitation of atomic beam current or atomic groups in a vacuum cavity can be guided by the reflection structure to be emitted towards one side of the photoelectric detection device, and particularly, the fluorescence which is not emitted towards one side of the photoelectric detection device is improved to be emitted again towards one side of the photoelectric detection device, and then the fluorescence is continuously received through a single photoelectric detection device, thereby improving the atomic group fluorescence collection proportion and improving the signal to noise ratio of quantum precision measurement detection.
As shown in fig. 1, the fluorescence detection system includes: the atomic beam generating device 1 is used for emitting an atomic beam 12, and the atomic beam 12 is a cylindrical atomic beam; the sealed vacuum cavity 2 is arranged at one side of the atomic beam emission device 1, and columnar atomic beams enter the sealed vacuum cavity 2 and are excited to generate fluorescence 13a and 13b, and the fluorescence 13a and 13b randomly emit into the sealed vacuum cavity 2; the reflecting structure 3 and the photoelectric detection device 4 are respectively positioned at the bottom 2a and the top 2b opposite to the closed vacuum cavity 2 in the direction perpendicular to the atomic beam 12; the reflecting structure 3 is configured to reflect the fluorescent light 13b emitted to the bottom 2a of the closed vacuum chamber 2, so that the fluorescent light 13b is reemitted to the top 2b of the closed vacuum chamber 2 and is received by the photoelectric detection device 4 together with the fluorescent light 13a located at the top 2b of the closed vacuum chamber 2.
In some embodiments, the reflective structure 3 and the photo detection device 4 are both located outside the closed vacuum chamber 2, so that no additional influence is exerted on the atomic beam 12 located in the closed vacuum chamber 2.
With continued reference to fig. 1, the reflective structure 3 is a reflective coating formed on the outside of the chamber wall at the bottom 2a of the closed vacuum chamber 2. The reflective coating may be formed outside the cavity wall of the bottom 2a by an application or plating process. The reflective coating may be a coating (film layer) of a metallic reflective material, wherein a reflective surface of the coating of a metallic reflective material contacts the outside of the cavity wall, facing the photo detection means 4.
The photo-detection devices 4 are attached to the outside of the closed vacuum chamber top 2b, and in some embodiments, only one photo-detection device 4 completely covers the fluorescence emission area of the closed vacuum chamber top 2b, so that all fluorescence emitted from the closed vacuum chamber top 2b can be accepted by the photo-detection device 4.
In this embodiment, after the fluorescence 13b emitted towards the bottom 2a of the closed vacuum chamber 2 is reflected by the reflective coating, the fluorescence is emitted towards the top 2b of the closed vacuum chamber 2 again, so that the proportion of fluorescence that can be detected by the photoelectric detection device 4 is increased; only one photoelectric detection device 4 is tightly attached to and completely covers the outer side of the top 2b of the closed vacuum cavity, so that fluorescence emitted from the top 2b of the closed vacuum cavity can be detected, and the signal to noise ratio of quantum precision measurement detection can be effectively improved.
As shown in fig. 1, the atomic beam generating device 1 further includes a collimation device 11, and the collimation device 11 collimates the atomic beam emitted by the atomic beam generating device through the collimation device 11 and emits the collimated atomic beam into the closed vacuum chamber 2, and forms a cylindrical atomic beam in the closed vacuum chamber 2.
As shown in fig. 2, the fluorescence detection system in the second embodiment of the present application is different from the fluorescence detection system in fig. 1 in that a concave mirror 5 is used as a reflecting structure. It should be understood that the same reference numerals in fig. 2 and fig. 1 denote similar elements with similar functions, and reference is made to the description in fig. 1 above, and redundant description is omitted.
With the concave mirror 5, the distance between the atomic beam 12 and the concave mirror 5 is approximately 1-2 times, preferably 2 times, the focal length of the concave mirror 5. In this embodiment, the atomic beam 12 is emitted from the collimator 11 to form a substantially cylindrical atomic beam, and the distance between the atomic beam 12 and the concave mirror 5 is approximately 1-2 times the focal length of the concave mirror 5, specifically, the perpendicular distance between the geometric center of the cylindrical atomic beam and the center of the concave surface of the concave mirror is approximately 1-2 times, preferably 2 times, the focal length of the concave mirror 5. It is understood that the following definitions of the distance between the atomic beam and the target in the present application refer to the vertical distance between the center of the atomic beam and the center of the surface of the target.
After the atomic beam 12 is irradiated by the laser, fluorescence 13b generated by atomic transition and emitted to the cavity wall at the bottom 2a of the closed vacuum cavity 2 is reflected and converged by the concave reflector 5, and is emitted to the top 2b of the closed vacuum cavity 2 again, and enters the photoelectric detection device 4 together with the fluorescence 13a emitted to the cavity wall at the top 2b of the closed vacuum cavity 2 in the atomic transition process, so that the detected fluorescence proportion can be improved, and the quantum precision measurement detection signal-to-noise ratio can be effectively improved.
As shown in fig. 3, the fluorescence detection system in the third embodiment of the present application is different from the fluorescence detection system in fig. 2 in that a first convex lens 6 is provided between the photo-detecting device 4 and the closed vacuum chamber 2. Specifically, the first convex lens 6 is located directly above the closed vacuum chamber 2 and directly below the photodetector 4; for example, the geometric center of the first convex lens 6 and the geometric center of the closed vacuum chamber 2, and the geometric center of the photodetector 4 are all on the same straight line. Further, the distance between the cylindrical atomic beam in the closed vacuum chamber 2 and the first convex lens 6 is about 1-2 times the focal length of the first convex lens 6, preferably 2 times the focal length of the first convex lens 6; the distance between the photodetector 4 and the first convex lens 6 is about 2 times or less the focal length of the first convex lens 6.
As shown in fig. 3, in the fluorescence detection system of this embodiment, the fluorescence 13b reflected and converged by the concave mirror 4 is reemitted and is emitted from the top 2b of the closed vacuum chamber 2 together with the fluorescence 13a of the top 2b of the closed vacuum chamber 2, and enters the photoelectric detection device 4 through the first convex lens 6 above the top 2b, so that the detected fluorescence proportion can be improved, and the signal to noise ratio of quantum precision measurement detection can be effectively improved. Specifically, when the fluorescence 13a and the reflected fluorescence 13b are located between the first focal length and the second focal length of the first convex lens 6, the fluorescence beam forms an inverted enlarged/equal-size image outside the second focal length of the other side of the first convex lens 6, the photoelectric detection device 4 is located near the second focal length of the first convex lens 6, and the first convex lens 6 plays a role in converging the light path, so that the receiving area of the photoelectric detection device 4 can be reduced, similar or better detection can be realized, and the signal to noise ratio can be improved.
As shown in fig. 4, the fluorescence detection system in the fourth embodiment of the present application is different from the fluorescence detection system in fig. 3 in that a second convex lens 6a and a plane mirror 7 are employed as a reflecting structure, wherein the second convex lens 6a is located between the bottom 2a of the closed vacuum chamber 2 and the plane mirror 4. Specifically, the second convex lens 6a and the plane mirror 7 are sequentially located right below the closed vacuum chamber 2, wherein the distance between the electron beam 12 and the second convex lens 6a is about 1 time the focal length of the second convex lens 6 a.
It will be appreciated that the geometric center of the second convex lens 6a, the geometric center of the closed vacuum chamber 2, and the geometric center of the plane mirror 7 are all on the same straight line. Further, since the geometric center of the first convex lens 6 and the geometric center of the photo-detecting device 4 are all on the same line with the geometric center of the closed vacuum chamber 2, in the fluorescence detecting system shown in fig. 4, the geometric centers of the photo-detecting device 6, the first convex lens 6, the closed vacuum chamber 2, the second convex lens 6a and the plane mirror 7 are all on the same line from top to bottom.
In addition, in this embodiment, after being excited by laser irradiation, the columnar atomic beam 12 in the closed vacuum chamber 2 is converged by the second convex lens 6a directly under the closed vacuum chamber 2, and then emitted to the plane mirror 7, and after being reflected, is converged by the second convex lens 6, and then emitted again to the top 2b of the closed vacuum chamber 2 and emitted to the photoelectric detection device 4 together with the fluorescent 13a at the top 2b of the closed vacuum chamber 2 after being converged by the first convex lens 6, and similarly, the detected fluorescence proportion and the quantum precision measurement detection signal to noise ratio can be improved effectively. Specifically, the atomic beam 12 is located at the focal length of the second convex lens 6a, the fluorescent light 13b emitted from the bottom is converged into parallel light in different directions through the second convex lens 6a, and then reflected by the plane mirror 7, and finally the fluorescent light 13b emitted from the bottom returns along the original light path, so that the divergence angle of the fluorescent light 13b emitted from the bottom is kept unchanged, and the fluorescent light 13b emitted to the top is converged through the first convex lens 6 together with the fluorescent light 13a emitted to the top, and then enters the photoelectric detection device 4. The second convex lens 6a and the plane reflecting mirror 7 greatly improve the fluorescence collection efficiency 5, and play a role of converging light paths through the first convex lens, meanwhile, the receiving area of the photoelectric detection device 4 is reduced, the local noise is reduced, and then the signal to noise ratio is greatly improved.
As shown in fig. 5, the fluorescence detection system in the fifth embodiment of the present application is different from the fluorescence detection system in fig. 1 in that a reflection prism structure 8 is adopted, and a flat side of the reflection prism structure 8 is fixedly connected to the outside of the cavity wall of the bottom 2a of the closed vacuum cavity 2 and is located outside the closed vacuum cavity 2; wherein the reflecting prismatic structure 8 comprises a plurality of micro corner cube arrays.
It should be noted that, in some embodiments of the present application, the reflecting prism structure 8 may also include only one triangular prism.
As can be seen from fig. 7, taking a triangular prism as an example, the fluorescent light 13b emitted from the bottom 2a of the closed vacuum chamber 2 forms an outgoing fluorescent light 13c parallel to and opposite to the outgoing light path under the action of the triangular prism, wherein the outgoing fluorescent light 13c is parallel and opposite after the incoming fluorescent light 13b is reflected by the triangular prism, but the incoming fluorescent light 13b and the outgoing fluorescent light 13c have a certain displacement L in the horizontal direction, and it can be understood that when the size of the triangular prism is reduced, the displacement Δl in the horizontal direction is also reduced. Therefore, if a plurality of micro pyramid prisms are used, the outgoing fluorescent light 13c parallel and opposite to the incoming fluorescent light 13b can be formed, and the horizontal displacement between the incoming fluorescent light 13b and the outgoing fluorescent light 13c is controlled, so that the parallel and opposite outgoing fluorescent light 13c can still enter the sealed vacuum chamber 2, and is detected by the photoelectric detection device 4 together with the fluorescent light 13a at the top 2b of the sealed vacuum chamber 2.
As shown in fig. 6, the fluorescence detection system in the fifth embodiment of the present application is different from the fluorescence detection system in fig. 5 in that a first convex lens 6 is provided between the photo-detecting device 4 and the closed vacuum chamber 2. At this time, the photodetector 4 is not closely attached to the outside of the top 2b of the closed vacuum chamber 2, and the first convex lens 6 collects the emitted fluorescence 13c in an opposite and parallel manner and the fluorescence 13a at the top 2b of the closed vacuum chamber 2, and then emits the fluorescence to the photodetector 4.
Wherein the first convex lens 6 is arranged between the photo-detecting device 4 and the closed vacuum chamber 2 (or the atomic beam 12), similar to the third embodiment of the present application, that is, the distance between the cylindrical atomic beam in the closed vacuum chamber 2 and the first convex lens 6 is about 1-2 times the focal length of the first convex lens 6, preferably about 2 times the focal length of the first convex lens 6; the distance between the photodetector 4 and the first convex lens 6 is about 2 times or less the focal length of the first convex lens 6.
It can be understood that when the relative positions among the atomic beam 12, the first convex lens 6 and the photoelectric detection device 4 are at the optimal positions, the photoelectric detection device 4 can be replaced by a single-point photoelectric detector (phi 0.1 mm), so that the effective detection of weak fluorescence can be realized, and the signal to noise ratio is greatly improved; conversely, if the relative positions of the atomic beam 12, the first convex lens 6 and the photodetector 4 do not match the above positional relationship, the receiving area of the photodetector 4 is correspondingly increased, so as to increase the detection effect of weak fluorescence.
In some embodiments of the application, in order to ensure that the fluorescence 13a, 13b can exit and enter the cavity walls of the top 2b and bottom 2a of the closed vacuum cavity 2, the cavity walls of the top and bottom are thus provided transparent, while the other cavity walls may be provided non-transparent or semi-transparent.
The top 2b and the bottom 2a of the closed vacuum chamber 2 in the present application are defined with reference to the current drawing, but are not limited thereto. That is, in other embodiments of the application, the top and bottom of the closed vacuum chamber may be interchanged or located in other relative positions when drawn with different viewing angles. In other words, the top and bottom only need to be arranged relatively in the vertical direction of the atomic beam, and when the atomic beam is emitted in the vertical direction, the left and right side walls of the closed vacuum chamber are the walls of the "top" and "bottom" defined above.
In summary, the application provides a fluorescence detection system, in the vertical direction of an atomic beam, a reflection structure and a photoelectric detection device are arranged on two opposite sides of the atomic beam, fluorescence emitted to the bottom cavity wall of a closed vacuum cavity is restrained to be emitted to the top cavity wall of the closed vacuum cavity again through the reflection structure, and the fluorescence emitted to the top of the closed vacuum cavity together with the fluorescence emitted to the top of the closed vacuum cavity is emitted to the photoelectric detection device from the top cavity wall of the closed vacuum cavity, so that the fluorescence signal collection difficulty generated by atomic transition is reduced, and the fluorescence collection rate is greatly improved. Meanwhile, as only one photoelectric detector is used, the requirements on the photoelectric detector are reduced, the cost is also reduced, and the fluorescence collection efficiency can be improved by 50-100%. In addition, a lens system (concave lens and convex lens) is added on a light path of fluorescence emitted from the top or bottom of the closed vacuum cavity, so that the fluorescence is converged, the requirement on the light passing caliber of the photoelectric detector is reduced, and the noise generated by the photoelectric detector is greatly reduced.
The application has been described with respect to the above-described embodiments, however, the above-described embodiments are merely examples of practicing the application. In addition, the technical features described above in the different embodiments of the present application may be combined with each other as long as they do not collide with each other. It should be noted that the disclosed embodiments do not limit the scope of the application. On the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the application.
Claims (10)
1. A fluorescence detection system, the fluorescence detection system comprising:
the atomic beam generating device is used for emitting atomic beam;
the closed vacuum cavity is arranged at one side of the atomic beam emission device, the atomic beam is excited in the closed vacuum cavity to generate fluorescence, and the fluorescence is randomly emitted to the inner wall of the closed vacuum cavity;
the reflecting structure and the photoelectric detection device are respectively positioned at the bottom and the top opposite to each other of the closed vacuum cavity in the direction perpendicular to the atomic beam current;
the reflection structure is used for reflecting the fluorescence emitted to the bottom of the closed vacuum cavity, so that the fluorescence is emitted to the top of the vacuum cavity again and is received by the photoelectric detection device.
2. The fluorescence detection system of claim 1, wherein the reflective structure is a reflective coating that covers the outside of the cavity wall at the bottom of the closed vacuum cavity and is located outside of the closed vacuum cavity.
3. The fluorescence detection system of claim 1, wherein the reflective structure is a reflective prism structure, and a flat side of the reflective prism structure is fixedly connected to an outer side of a cavity wall at the bottom of the closed vacuum cavity and is positioned at an outer side of the closed vacuum cavity; the reflecting prism structure comprises a single pyramid prism or a pyramid prism array formed by a plurality of miniature pyramid prisms.
4. The fluorescence detection system of claim 1, wherein the reflecting structure is a concave mirror and the distance between the atomic beam and the concave mirror is 1-2 times the focal length of the concave mirror.
5. The fluorescence detection system of claim 1, wherein the reflective structure is a combined reflective structure of a second convex lens and a planar mirror, the second convex lens being located between an outside of a bottom of the closed vacuum chamber and a reflective plane of the planar mirror, a distance between the second convex lens and the atomic beam is equal to a focal length of the second convex lens;
and fluorescence emitted to the bottom of the closed vacuum cavity is converged by the second convex lens, continuously emitted to the reflection plane, reflected by the reflection plane, then emitted upwards by the second convex lens, emitted from the top of the closed vacuum cavity and received by the photoelectric detection device.
6. The fluorescence detection system of any one of claims 2-5, further comprising a first convex lens disposed between the top of the closed vacuum chamber and the photodetection device, wherein the fluorescence emitted from the top of the closed vacuum chamber is received by the photodetection device after being converged by the convex lens;
the distance between the first convex lens and the atomic beam current is 1-2 times of the focal length of the first convex lens.
7. The fluorescence detection system of claim 6, wherein a distance between the first convex lens and the photodetection device is equal to or less than twice a focal length of the first convex lens.
8. The fluorescence detection system of any one of claims 2-5, wherein the photodetection device is affixed to the outside of the chamber wall at the top of the closed vacuum chamber.
9. The fluorescence detection system of claim 8, wherein the photodetection device completely covers the outside of the chamber wall at the top of the closed vacuum chamber.
10. The fluorescence detection system of any one of claims 3-5, wherein the opposing top and bottom chamber walls of the closed vacuum chamber are configured to be transparent.
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