CN117045855A - Biological bionic hydrogel film liquid and preparation method thereof - Google Patents
Biological bionic hydrogel film liquid and preparation method thereof Download PDFInfo
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- CN117045855A CN117045855A CN202311069479.XA CN202311069479A CN117045855A CN 117045855 A CN117045855 A CN 117045855A CN 202311069479 A CN202311069479 A CN 202311069479A CN 117045855 A CN117045855 A CN 117045855A
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- 239000000017 hydrogel Substances 0.000 title claims abstract description 37
- 239000007788 liquid Substances 0.000 title claims abstract description 26
- 239000011664 nicotinic acid Substances 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title abstract description 19
- 102000008186 Collagen Human genes 0.000 claims abstract description 40
- 108010035532 Collagen Proteins 0.000 claims abstract description 40
- 229920001436 collagen Polymers 0.000 claims abstract description 40
- 102000015636 Oligopeptides Human genes 0.000 claims abstract description 29
- 108010038807 Oligopeptides Proteins 0.000 claims abstract description 29
- 239000012528 membrane Substances 0.000 claims abstract description 15
- 229920002385 Sodium hyaluronate Polymers 0.000 claims abstract description 9
- 229940010747 sodium hyaluronate Drugs 0.000 claims abstract description 9
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 9
- SNPLKNRPJHDVJA-ZETCQYMHSA-N D-panthenol Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCCO SNPLKNRPJHDVJA-ZETCQYMHSA-N 0.000 claims abstract description 8
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 claims abstract description 8
- 229960004308 acetylcysteine Drugs 0.000 claims abstract description 8
- 229940101267 panthenol Drugs 0.000 claims abstract description 8
- 239000011619 pantothenol Substances 0.000 claims abstract description 8
- 235000020957 pantothenol Nutrition 0.000 claims abstract description 8
- 230000003592 biomimetic effect Effects 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 12
- 239000012452 mother liquor Substances 0.000 claims description 11
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 4
- 229910021641 deionized water Inorganic materials 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims 1
- 239000012669 liquid formulation Substances 0.000 claims 1
- 230000008929 regeneration Effects 0.000 abstract description 8
- 238000011069 regeneration method Methods 0.000 abstract description 8
- 230000008439 repair process Effects 0.000 abstract description 6
- 239000003963 antioxidant agent Substances 0.000 abstract description 3
- 230000003078 antioxidant effect Effects 0.000 abstract description 3
- 239000012620 biological material Substances 0.000 abstract description 3
- 238000010276 construction Methods 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 210000004761 scalp Anatomy 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 47
- 238000000034 method Methods 0.000 description 8
- 239000004964 aerogel Substances 0.000 description 6
- 230000007547 defect Effects 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 3
- 108050007372 Fibroblast Growth Factor Proteins 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 230000002500 effect on skin Effects 0.000 description 3
- 229940126864 fibroblast growth factor Drugs 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000002054 transplantation Methods 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003937 drug carrier Substances 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 229910021389 graphene Inorganic materials 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 1
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 230000009193 crawling Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0033—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Birds (AREA)
- Materials Engineering (AREA)
- Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Dermatology (AREA)
- Medicinal Preparation (AREA)
Abstract
The application relates to the technical field of scalp care products, in particular to a biological bionic hydrogel film liquid and a preparation method thereof, wherein the biological bionic hydrogel film liquid comprises the following components: collagen oligopeptide, N-acetylcysteine, sodium hyaluronate, panthenol and liquid preparation. According to the biological bionic hydrogel membrane liquid and the preparation method thereof, a scaffold construction strategy of tissue engineering regeneration medicine is adopted, and the sodium hyaluronate and collagen oligopeptide are utilized to form a biological bionic hydrogel membrane suitable for skin repair on the surface of skin, so that the skin can resist external stimulus more intensively, the skin collagen regeneration is activated, the self-repair space of the skin is provided, and the self-healing force of the skin is improved by several times; the bionic hydrogel film adopts a plurality of special preparations with refractive biological materials, so that a hydrogel film layer is formed on the surface of the skin in a microcosmic mode by film liquid, the skin is transparent and glossy by refracting light, and meanwhile, the skin transparent and glossy is improved from the bottom layer by matching with an antioxidant.
Description
Technical Field
The application relates to the technical field of skin care products, in particular to a biological bionic hydrogel film liquid and a preparation method thereof.
Background
Skin defects caused by large-area deep burn, wound and the like are common and frequently encountered diseases in clinic, and are characterized by large skin defect areas and accompanied by full-thickness skin defects of different degrees. The conventional treatment means in clinic at present are autologous medium thickness skin, full thickness skin transplantation, allogenic decellularized dermal matrix composite autologous epidermis transplantation and xenogenic decellularized dermal matrix composite autologous epidermis transplantation. Autologous skin grafting can reconstruct the skin structure at the defect site and restore its appearance and function, but this method lacks sufficient skin source and can also add pain to the patient. The allogenic decellularized dermal matrix composite autologous skin grafting method also has the defect of limited sources of allogenic skin donors, and has the possibility of transmitting human diseases and ethical problems.
At present, research on promoting healing of difficult-to-heal wounds is focused on two aspects of scaffold materials (such as collagen) and drug carriers (such as bFGF, fibroblast growth factor). Among the numerous tissue engineering materials, collagen is widely accepted as a natural protein that is widely present in animal skin, bone, tendon ligament and cornea tissue, as a drug carrier in tissue engineering and to construct active three-dimensional scaffolds. However, collagen has many limitations as a scaffold material, collagen can only be dissolved in an acidic environment in an in-vitro preparation process, water-soluble proteins, cytokines and other nutritional ingredients are difficult to disperse uniformly in a neutral environment, and the acidic environment also easily causes inactivation of cell active factors, so that in an in-vitro co-culture process with cells, the nutritional ingredients such as growth factors in a culture medium are difficult to diffuse into the scaffold material, and nutrition can not be provided for the cells.
For example, the chinese patent application No. 201810031650.0 discloses a preparation method and application of a skin repair aerogel dressing, which uses collagen and graphene oxide as the basis, uses a preparation process of aerogel, uses a collagen film with a relatively dense collagen-graphene oxide aerogel surface to slowly release fibroblast growth factor through physical wrapping and a sandwich-type multi-layer structure assembly mode, and adopts a freeze-drying method to prepare an aerogel dressing which can slowly release fibroblast growth factor, has uniform pore distribution, light texture, good moisture absorption, moisture preservation, water absorption, air permeability and porosity, can promote crawling and adhesion of fibroblast in skin, and can promote wound healing.
With respect to the related art in the above, the inventors still consider that the following drawbacks exist:
the aerogel dressing is complex in preparation process and high in manufacturing cost, and in the using process, skin cannot be promoted to resist external stimulus, skin collagen regeneration is difficult to activate, the self-repairing space of the skin is smaller, the self-healing force of the skin is poorer, more importantly, the skin cannot be transparent, and the skin transparent brightness is difficult to be improved from the bottom layer, so that improvement is needed.
Disclosure of Invention
The application provides a bionical hydrogel membrane liquid and a preparation method thereof, which are used for improving the following technical problems:
the aerogel dressing is complex in preparation process and high in manufacturing cost, and in the using process, skin cannot be promoted to resist external stimulus, skin collagen regeneration is difficult to activate, the self-repairing space of the skin is smaller, the self-healing force of the skin is poorer, more importantly, the skin cannot be transparent, and the skin transparent brightness is difficult to be improved from the bottom layer.
In a first aspect, the present application provides a biomimetic hydrogel membrane solution, which adopts the following technical scheme:
the bionic hydrogel film liquid comprises the following components in parts by weight:
in one realizable technical scheme of the application, the application comprises the following components in parts by weight:
in one technical scheme of the application, the collagen oligopeptide is collagen oligopeptide with molecular weight of 100-1000Da, and is formed by condensing 2-10 amino acids.
In one possible technical solution of the present application, the liquid preparation is a single component or a complex of multiple components of the following materials: water, glycerol, propylene glycol.
In a second aspect, the present application provides a method for producing the biomimetic hydrogel membrane liquid, which adopts the following technical scheme:
a method for preparing a biomimetic hydrogel membrane liquid, comprising the following steps:
(1) Slowly adding collagen oligopeptide into deionized water with the mass of 5-10 times of that of the raw materials, and slowly rotating and dissolving in a magnetic stirrer at the rotating speed of 100-200r/min and the temperature of 75-85 ℃ for 2 hours to slowly cool the collagen oligopeptide to obtain collagen oligopeptide mother liquor;
(2) Cooling the collagen oligopeptide mother liquor to 48-52 ℃, then adding the collagen oligopeptide mother liquor into sodium hyaluronate, fully stirring and dissolving for 2 hours under the rotating speed condition of 300-400r/min, then adding N-acetylcysteine and panthenol, and stirring until the collagen oligopeptide mother liquor is fully dissolved;
(3) Cooling to normal temperature, and standing to obtain the biomimetic hydrogel membrane liquid.
In summary, the working principle and the beneficial technical effects of the application are as follows:
the scaffold construction strategy of tissue engineering regeneration medicine is adopted, sodium hyaluronate and collagen oligopeptide are utilized to form a biological bionic hydrogel film suitable for skin repair on the surface of the skin, the biological bionic hydrogel film can enable the skin to resist external stimulation more intensively, activate skin collagen regeneration, provide self-repair space for the skin and improve skin self-healing force by several times;
the bionic hydrogel film adopts a plurality of special preparation of refractive biological materials (namely collagen oligopeptide, N-acetylcysteine, sodium hyaluronate, panthenol and water), so that a layer of hydrogel film is formed on the surface of the skin in a microcosmic mode, water is refracted through light rays to moisten and shiny skin, meanwhile, the skin is improved in brightness through the bottom layer by matching with an antioxidant, the cooperation of the N-acetylcysteine and the panthenol has good dilution and anti-inflammation effects, and the skin repair is well promoted.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is an electron microscopy view of a biomimetic hydrogel film according to an embodiment of the present application.
Detailed Description
In order to make the technical problems, technical schemes and beneficial effects to be solved more clear, the application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the application.
It will be understood that when an element is referred to as being "mounted" or "disposed" on another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
It is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are merely for convenience in describing and simplifying the description based on the orientation or positional relationship shown in the drawings, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus are not to be construed as limiting the application.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
The present application will be described in further detail with reference to fig. 1.
The embodiment of the application discloses a biomimetic hydrogel membrane liquid. Referring to fig. 1, the biomimetic hydrogel membrane liquid adopts the following technical scheme:
the bionic hydrogel film liquid comprises the following components in parts by weight:
in a preferred embodiment of the present application, the biomimetic hydrogel film liquid comprises the following components in parts by weight:
in this embodiment, the collagen oligopeptide is specifically a collagen oligopeptide with a molecular weight of 100-1000Da, and is formed by condensing 2-10 amino acids.
In this embodiment, the liquid preparation is water or deionized water, and in other embodiments, the liquid preparation may be glycerin or propylene glycol, or a complex of multiple materials in water, glycerin, and propylene glycol.
The application also provides a preparation method of the bionic hydrogel membrane liquid, which adopts the following technical scheme:
a method for preparing a biomimetic hydrogel membrane liquid, comprising the following steps:
(1) Slowly adding collagen oligopeptide into deionized water with the mass of 5-10 times of that of the raw materials, and slowly rotating and dissolving in a magnetic stirrer at the rotating speed of 100-200r/min and the temperature of 75-85 ℃ for 2 hours to slowly cool the collagen oligopeptide to obtain collagen oligopeptide mother liquor;
(2) Cooling collagen oligopeptide mother liquor to 48-52 ℃, then adding the collagen oligopeptide mother liquor into sodium hyaluronate, fully stirring and dissolving for 2 hours at the rotating speed of 300-400r/min, then adding N-acetylcysteine and panthenol, and at the moment, adding other skin conditioning agents (such as basic formulas of cream, essence toner and the like) and preservatives and the like according to the need, and stirring and dissolving fully;
(3) Cooling to normal temperature, and standing to obtain the biomimetic hydrogel membrane liquid.
In summary, the working principle and the beneficial technical effects of the application are as follows:
the scaffold construction strategy of tissue engineering regeneration medicine is adopted, sodium hyaluronate and collagen oligopeptide are utilized to form a biological bionic hydrogel film suitable for skin repair on the surface of the skin, the biological bionic hydrogel film can enable the skin to resist external stimulation more intensively, activate skin collagen regeneration, provide self-repair space for the skin and improve skin self-healing force by several times;
the bionic hydrogel film adopts a plurality of special preparation of refractive biological materials (namely collagen oligopeptide, N-acetylcysteine, sodium hyaluronate, panthenol and water), so that a layer of hydrogel film is formed on the surface of the skin in a microcosmic mode, water is refracted through light rays to moisten and shiny skin, meanwhile, the skin is improved in brightness through the bottom layer by matching with an antioxidant, the cooperation of the N-acetylcysteine and the panthenol has good dilution and anti-inflammation effects, and the skin repair is well promoted.
The foregoing description of the preferred embodiments of the application is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the application.
Claims (5)
1. The bionic hydrogel membrane liquid is characterized by comprising the following components in parts by weight:
2. the biomimetic hydrogel film according to claim 1, comprising the following components in parts by weight:
3. the biomimetic hydrogel membrane fluid of claim 1, wherein the collagen oligopeptide is a collagen oligopeptide with a molecular weight between 100-1000Da, and is formed by condensing 2-10 amino acids.
4. The biomimetic hydrogel film liquid of claim 1, wherein the liquid formulation is a single component or a complex of components therein of the following materials: water, glycerol, propylene glycol.
5. A method of making a biomimetic hydrogel film as recited in any one of claims 1 to 4, comprising the steps of:
(1) Slowly adding collagen oligopeptide into deionized water with the mass of 5-10 times of that of the raw materials, and slowly rotating and dissolving in a magnetic stirrer at the rotating speed of 100-200r/min and the temperature of 75-85 ℃ for 2 hours to slowly cool the collagen oligopeptide to obtain collagen oligopeptide mother liquor;
(2) Cooling the collagen oligopeptide mother liquor to 48-52 ℃, then adding the collagen oligopeptide mother liquor into sodium hyaluronate, fully stirring and dissolving for 2 hours under the rotating speed condition of 300-400r/min, then adding N-acetylcysteine and panthenol, and stirring until the collagen oligopeptide mother liquor is fully dissolved;
(3) Cooling to normal temperature, and standing to obtain the biomimetic hydrogel membrane liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202311069479.XA CN117045855A (en) | 2023-08-23 | 2023-08-23 | Biological bionic hydrogel film liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311069479.XA CN117045855A (en) | 2023-08-23 | 2023-08-23 | Biological bionic hydrogel film liquid and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117045855A true CN117045855A (en) | 2023-11-14 |
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|---|---|---|---|
| CN202311069479.XA Pending CN117045855A (en) | 2023-08-23 | 2023-08-23 | Biological bionic hydrogel film liquid and preparation method thereof |
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| Country | Link |
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