CN117019253A - Liquid transferring pump device and use method thereof - Google Patents
Liquid transferring pump device and use method thereof Download PDFInfo
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- CN117019253A CN117019253A CN202310883097.4A CN202310883097A CN117019253A CN 117019253 A CN117019253 A CN 117019253A CN 202310883097 A CN202310883097 A CN 202310883097A CN 117019253 A CN117019253 A CN 117019253A
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- 239000007788 liquid Substances 0.000 title claims abstract description 127
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000006698 induction Effects 0.000 claims abstract description 13
- 230000001360 synchronised effect Effects 0.000 claims abstract description 4
- 230000007246 mechanism Effects 0.000 claims description 36
- 238000007789 sealing Methods 0.000 claims description 24
- 230000008859 change Effects 0.000 claims description 22
- 238000004891 communication Methods 0.000 claims description 9
- 239000000523 sample Substances 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 5
- 238000010521 absorption reaction Methods 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 2
- 238000012546 transfer Methods 0.000 description 10
- 238000001514 detection method Methods 0.000 description 8
- 230000000903 blocking effect Effects 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 5
- 150000007523 nucleic acids Chemical class 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 5
- 238000013461 design Methods 0.000 description 4
- 230000004888 barrier function Effects 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 230000004907 flux Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0227—Details of motor drive means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0237—Details of electronic control, e.g. relating to user interface
Abstract
The invention discloses a liquid transferring pump device, a using method and application thereof, and the liquid transferring pump device comprises a first liquid transferring pump, a second liquid transferring pump and an induction device, wherein the first liquid transferring pump is used for completing the suction and the discharge of liquid with smaller volume, the second liquid transferring pump is used for completing the suction and the discharge of liquid with larger volume, the first liquid transferring pump is provided with a maximum volume, a channel is arranged at the maximum volume and is communicated with the second liquid transferring pump, the induction device is communicated with a liquid transferring channel of the first liquid transferring pump, and the pressure in the liquid transferring channel is detected to control the stop of the first liquid transferring pump and the synchronous start of the operation of the second liquid transferring pump. The liquid transferring pump device, the using method and the application thereof provided by the invention have the advantages of simple structure, low cost and strong practicability, and can meet the development requirements of an automatic platform compatible with large and small liquid volumes in IVD direction sample volume and methodology.
Description
Technical Field
The invention relates to the technical field of biochemical detection experiments, in particular to a liquid transferring pump device and a using method thereof.
Background
In the prior art in the field of In Vitro Diagnostics (IVD), the required parameters are measured by varying degrees of automation, wherein the pipetting process mostly uses pipetting pumps for transferring and dispensing liquids, the high throughput automated liquid handling protocol is applicable with small volumes (10-1000 ul) of liquid, while the liquid handling protocol with high volumes of liquid is generally less accurate, easily contaminated and has a low degree of automation, thus limiting the development of compatible-sized liquid handling platforms in IVD-oriented sample volumes and methodologies.
In the automatic nucleic acid detection aiming at the high sample volume and the compatible liquid volume requirements (such as gene detection, nucleic acid extraction of a biological sample library and mixed detection of a plurality of samples) in the methodology, the high-precision requirements during small-volume pipetting are not reduced, and meanwhile, the transfer of large-volume liquid can be considered, but the prior art cannot meet the requirements.
In particular, the nucleic acid detection in a fully automatic or pipeline mode usually needs to be performed respectively for nucleic acid extraction, detection reaction volume construction and amplification analysis, so that the method is limited by the prior art, at least three devices are required to contain three different pipetting devices for realizing, and the connection is difficult due to the special properties of the detection kit, so that the cost and the technical barrier of the fully automatic or pipeline nucleic acid detection are high.
In the prior art, the pipetting devices are roughly divided into three cases: firstly, a single plunger in the pump body is independently driven, and the pump is applied to operation with high requirements on small and medium volume accuracy. Defects: single application and poor self-adaptability. And secondly, a plurality of plungers in the pump body are connected in parallel with a linear mechanism for driving, so that the pump is applied to medium-small-volume high-efficiency operation, and the number of channels is 96, 192 or more according to the application requirements. Defects: high cost, extremely high debugging requirements and precision requirements (such as parallelism and flatness), huge volume, pseudo-large volume and easy pollution. In addition, the traditional plastic or glass cavity and the plastic plunger have wide application range and can realize large volume and small volume. Defects: the small-volume pipetting accuracy is low, the dead volume influence is large, the automation degree is extremely low, the speed is low, the flux is low, the volume is large, the service life is short, and the different volumes are required to be switched.
Accordingly, there is a need to provide a pipetting device, a method of using the same and an application thereof to address the drawbacks of the prior art.
Disclosure of Invention
The invention provides a liquid transferring pump device, a using method and application thereof, which are simple in structure, low in cost and high in practicability by improving the existing liquid transferring pump device, the using method and application thereof.
In order to achieve the above purpose, one of the technical schemes adopted by the invention is as follows: the utility model provides a pipetting device, includes first pipetting pump, second pipetting pump and induction system, and less volume liquid is absorbed and discharged to first pipetting pump, and more volume liquid is absorbed and discharged to second pipetting pump, and first pipetting pump is provided with the biggest volume, and biggest volume department sets up the passageway and communicates with the second pipetting pump, induction system intercommunication first pipetting pump's pipetting channel, detects pressure in the pipetting channel comes the stop of control first pipetting pump and the operation of synchronous start second pipetting pump.
Preferably, the first liquid transferring pump comprises a first cylinder body, a piston rod and a first driving mechanism, the piston rod is movably arranged in the first cylinder body under the driving of the first driving mechanism, a first through hole is formed in the center of the bottom of the piston rod, a second through hole is formed in one end, close to the bottom, of the piston rod, and the first through hole is communicated with the second through hole.
Preferably, the first cylinder body comprises a cylinder body base, an air passage adapter and a cylinder cover, wherein the cylinder body base, the air passage adapter and the cylinder cover share a central axis through hole, and the piston rod reciprocates in the cylinder body base, the air passage adapter and the cylinder cover share the central axis through hole.
Preferably, the sensing device comprises a pressure sensor, the pressure sensor is arranged on one side of the bottom of the cylinder base, and the pressure sensor is communicated with the cylinder base, the gas circuit adapter and the cylinder cover through a pipeline to form a coaxial axis through hole.
Preferably, the cylinder body base upper portion and cylinder cap lower part are column type evagination structure, and gas circuit adapter both ends are column type indent structure, and indent structure and evagination structure are central axis and cooperation form first cylinder body, and evagination structure length is less than indent structure degree of depth, and gas circuit adapter and cylinder body base, cylinder cap junction are provided with first sealing washer, second sealing washer, third sealing washer and fourth sealing washer respectively.
Preferably, a transverse through hole is formed in the transverse center of the air passage adapter, the transverse through hole is the largest volume of the first liquid transferring pump, a longitudinal through hole is formed in one side of the air passage adapter, the longitudinal through hole is communicated with the transverse through hole, the piston rod reaches the largest volume, and the second through hole is communicated with the transverse through hole.
Preferably, the second liquid transferring pump comprises a pipeline, a second cylinder body, a second piston rod and a second driving mechanism, one end of the pipeline is communicated with the longitudinal through hole, the other end of the pipeline is communicated with the second cylinder body, a communication channel of the first liquid transferring pump and the second liquid transferring pump is formed, and the second piston rod is driven by the second driving mechanism to reciprocate in the second cylinder body to complete liquid suction and liquid discharge.
Preferably, the liquid transferring pump device comprises a housing, the housing comprises a first housing and a second housing, the second housing is connected to the top of the first housing, the first housing is a mechanical part housing, the second housing is a control part housing, a through hole groove communicated with the first cylinder body is formed in the bottom of the first housing, and a gun head adapter for liquid transferring is arranged at the through hole groove.
The invention also provides application of the pipetting device in sample pipetting, which comprises a small-volume pipetting mode and a large-volume pipetting mode, wherein the system operates in the small-volume accurate pipetting mode under the condition of small-volume liquid of 10-1000ul liquid; and when the volume of the liquid exceeds a small volume (such as 10-1000 ul), the liquid is transferred into a large-volume liquid transferring mode, and the small volume is transferred into the large volume by adopting the combination of self-adaptive gas-liquid conversion.
The third technical scheme adopted by the invention is as follows: a pipetting method of a pipetting device comprises the steps of completing pipetting with large volume and high precision by adopting a pump, wherein the pipetting with small volume and pipetting with large volume are completed, the pipetting process with small volume is controlled fully automatically, the pipetting amount is 10-1000ul of liquid with small volume, an induction device is in circuit communication with a control module, a sensor in the induction device provides signals for the control module to obtain whether a piston rod is at an origin position, so that the starting position of the movement of the piston rod is ensured to be consistent every time, whether a driving mechanism operates normally is detected, and the change of the position of an adapter sleeve is obtained: when the gun head is taken out, the adapter sleeve extrudes the elastic mechanism to move upwards, position signal change is provided to determine that the gun head is taken out, when the gun head is taken out, the adapter sleeve downwards moves the elastic mechanism to restore the original position, position signal change is provided to determine that the gun head is taken out, and air pressure change is sealed in the closed cavity through the pressure sensor probe to sense an air pressure value; the process of large-volume pipetting adopts full automatic control, when the large-volume liquid of more than 1000uL is required to be sucked and discharged, the first piston rod continuously moves to the position where the second through hole of the piston rod coincides with the transverse through hole, meanwhile, the pressure sensor senses the pressure change, the first piston rod stops moving, and the second pipetting pump is synchronously started to move so as to provide the pressure for sucking and discharging the large-volume liquid.
The invention has the beneficial effects that:
by adopting the technical scheme, the invention automatically judges and executes switching of the combined pump (the second pump) according to the liquid amount input data to be processed, so that the combined pump can complete the target, the gas-liquid conversion design is realized, the liquid transfer precision is high, the application range is wide, and the automation degree is high; one pump is multipurpose, the pipetting range is wide and the seamless connection is realized, and the technical barrier of the full automation requirement of the high-flux liquid workstation on the large-volume pipetting is met; the modular design is adopted, the electric part and the mechanical part are independently integrated, so that the cost is greatly reduced while the diversified components are optimized; the pump body is compact in structure, the pump body meets the requirement of large-volume pipetting, the volume is small, the minimum arrangement distance is 9mm, and the automation requirement of the large-volume high-flux existing commercial mode is realized.
Drawings
In order to more clearly illustrate the embodiments of the present invention and the advantages of the present invention, the following description will briefly explain the embodiments or the drawings used in the description of the prior art, and it is apparent that the drawings in the following description are only some embodiments of the present invention and that other structures may be obtained according to the structures shown in these drawings without inventive effort to those skilled in the art.
FIG. 1 is a schematic diagram of the structure of the present invention.
FIG. 2 is a second schematic diagram of the structure of the present invention.
Fig. 3 is a cross-sectional view of A-A in fig. 1.
Fig. 4 is a cross-sectional view of B-B of fig. 1.
Fig. 5 is a schematic structural view of a first liquid transfer pump according to the present invention.
Fig. 6 is a schematic view showing the structure of the first pipetting pump of the invention with its housing removed.
Fig. 7 is a schematic view of the structure of fig. 6 with the first piston rod up to the maximum volume.
Reference numerals illustrate: the device comprises a first liquid transfer pump-1, a second liquid transfer pump-2, an air pipe-3, a gun head adapter-4, a first driving motor-5, a screw rod assembly-6, a movable bridge seat-7, a second driving mechanism-8, a second screw rod 9, an adapter sleeve assembly-10, a second light blocking piece-11, a piston rod 12, a shell 13, a first sealing ring 15, a second sealing ring 16, a third sealing ring 17, a fourth sealing ring 18, a pressure sensor 19, a through hole groove 20, a first through hole 21, a transverse through hole 22, a guide post 25 and a first light blocking piece-26;
the cylinder body base 1-1, the gas circuit adapter 1-2 and the cylinder cover 1-3.
Detailed Description
According to an implementation scheme of a liquid transferring pump device, the liquid transferring pump device comprises a first liquid transferring pump 1, a second liquid transferring pump 2 and an induction device, wherein the first liquid transferring pump is used for completing liquid suction and discharge of a small volume amount, the second liquid transferring pump is used for completing liquid suction and discharge of a large volume amount, the first liquid transferring pump is provided with a maximum volume, a channel is arranged at the maximum volume and is communicated with the second liquid transferring pump, the induction device is communicated with a liquid transferring channel of the first liquid transferring pump, and pressure in the liquid transferring channel is detected to control the stop of the first liquid transferring pump and the synchronous start of the operation of the second liquid transferring pump.
Preferably, the first liquid transferring pump comprises a first cylinder body, a piston rod 12 and a first driving mechanism, the piston rod is movably arranged in the first cylinder body under the driving of the first driving mechanism, a first through hole 21 is arranged at the center of the bottom of the piston rod, a second through hole is arranged at one end, close to the bottom, of the piston rod, and the first through hole is communicated with the second through hole.
Preferably, the first cylinder body comprises a cylinder body base 1-1, an air passage adapter body 1-2 and a cylinder cover 1-3, wherein the cylinder body base, the air passage adapter body and the cylinder cover are coaxial with a central axis through hole, and the piston rod reciprocates in the cylinder body base, the air passage adapter body and the cylinder cover are coaxial with the central axis through hole.
Preferably, the sensing device comprises a pressure sensor 19, wherein the pressure sensor is arranged on one side of the bottom of the cylinder base, and the pressure sensor is communicated with the cylinder base, the gas circuit adapter and the cylinder cover through holes with the central axis.
Preferably, the cylinder body base upper portion and cylinder cap lower part are column type evagination structure, and the gas circuit adapter both ends are column type indent structure, indent structure and evagination structure concentric axis and cooperation form first cylinder body, and evagination structure length is less than indent structure degree of depth, and the gas circuit adapter is provided with first sealing washer 15, second sealing washer 16, third sealing washer 17 and fourth sealing washer 18 respectively with cylinder body base, cylinder cap junction.
Preferably, a transverse through hole 22 is arranged at the transverse center of the air passage adapter, the transverse through hole is the maximum volume of the first liquid transferring pump, a longitudinal through hole is arranged on one side of the air passage adapter, the longitudinal through hole is communicated with the transverse through hole, the piston rod reaches the maximum volume, and the second through hole is communicated with the transverse through hole.
Preferably, the second liquid transferring pump comprises a pipeline, a second cylinder body, a second piston rod and a second driving mechanism, one end of the pipeline is communicated with the longitudinal through hole, the other end of the pipeline is communicated with the second cylinder body, a communication channel of the first liquid transferring pump and the second liquid transferring pump is formed, and the second piston rod is driven by the second driving mechanism to reciprocate in the second cylinder body to complete liquid suction and liquid discharge.
Preferably, the liquid transferring pump device comprises a housing 13, the housing comprises a first housing and a second housing, the second housing is connected to the top of the first housing, the first housing is a mechanical part housing, the second housing is a control part housing, a through hole groove 20 communicated with the first cylinder body is formed in the bottom of the first housing, and a gun head adapter 4 for liquid transferring is arranged at the through hole groove.
Specific examples of the invention:
as shown in fig. 1 to 7, the liquid transferring pump device of the present embodiment is a pump connecting device of a first liquid transferring pump and a second liquid transferring pump, and the liquid transferring pump of the present embodiment adopts an air pump device, and the first liquid transferring pump comprises a housing, an air pipe 3 (as a pipeline for connecting the second liquid transferring pump), a driving mechanism, and an induction device.
The shell of the embodiment consists of two parts, including a mechanical part shell and a control part shell, wherein the bottom of the mechanical part shell is provided with a positioning device, the bottom of the shell is provided with a through hole groove, and the side wall of the shell is provided with an air passage joint. The control part shell is connected to the top of the mechanical part shell, and the shell is provided with a butt plug relative to the contact part.
The device of the embodiment is composed of a first pipetting pump 1, a second pipetting pump 2, a gun head adapter 4 and an adapter sleeve assembly. The pump body component is accommodated in the mechanical part shell and fixed on the shell, the adapter is connected with the pump body component and extends out from the bottom of the mechanical part shell, the adapter sleeve is coated on the adapter and is positioned on the same central axis, and the second liquid transferring pump 2 is connected with the first liquid transferring pump body 1 through an air pipe.
The driving mechanism of the embodiment comprises a first driving mechanism and a second driving mechanism, wherein the first driving mechanism is in power connection with a first liquid transferring pump body component, the second driving mechanism is in power connection with a second liquid transferring pump body component, the first driving mechanism is positioned inside a mechanical shell and consists of a first driving motor 5, a screw rod component 6 and a guide post 25, the first driving motor is connected with the screw rod mechanism 6 to provide liquid sucking and discharging driving force for the pump body component, the guide post penetrates through a screw rod component connecting block and is parallel to the screw rod and is in the same plane, the guide post is connected with the shell and the pump body component, the guide post of the embodiment is a hollow tube, one end of the guide post is connected with an air pipe, and the other end of the guide post is communicated with the air pipe. The second driving mechanism consists of a second driving motor 8 and a screw rod assembly, and the second driving motor 2 is connected with the screw rod 9 to provide the driving force of the pump body assembly for sucking and discharging liquid.
The sensing device of the embodiment comprises a pressure sensor 19, wherein the pressure sensor 19 is connected with a pump body component and is positioned in a mechanical shell.
The first liquid transferring pump 1 of the embodiment comprises a first cylinder body and a first piston rod 12, wherein the first cylinder body consists of a base 1-1, a gas circuit adapter body 1-2 and a cylinder cover 1-3, the base of the cylinder body, the gas circuit adapter body and the cylinder cover share a central axis through hole, a connecting hole is formed in the outer portion of the base of the cylinder body, and the connecting hole is connected with a pressure sensor 19 and provided with a sealing ring.
In the embodiment, the upper part of the base and the lower part of the cylinder cover are of column-type convex structures, two ends of the gas circuit adapter are of column-type concave structures, the concave and convex share the same central axis, and the convex cylinder can be inserted into the concave body, so that the length of the convex cylinder is smaller than the depth of the concave.
In the column-shaped concave body of the gas circuit adapter of the embodiment, a first sealing ring 15 and a fourth sealing ring 18 are respectively designed to form a sealing ring.
The transverse center of the gas circuit adapter is provided with a through hole which is connected with the longitudinal through hole to form a three-way structure, and the through hole at the transverse center is connected with the second liquid transferring pump. The outer convex column body on the upper part of the base and the lower part of the cylinder cover is provided with two sections of grooves, and the grooves are respectively provided with a second sealing ring and a third sealing ring to form a sealing ring.
The piston rod and the base, the gas path adapter, the cylinder cover and the cylinder body formed by the second transfer pump form a closed cavity, the piston rod reciprocates in the closed cavity in a linear motion, a through hole is arranged at the center of the bottom end of the piston rod, and the other end of the through hole is positioned at the side surface of the piston rod.
The second liquid transferring pump also adopts an air pump structure and consists of a cylinder body and a piston rod, wherein the cylinder body is provided with a central through hole, one end of the cylinder body is connected with the piston rod, and the other end is communicated with the air passage adapter.
The screw rod assembly 6 comprises a screw rod, a screw rod nut and a movable bridge seat 7, the screw rod is connected with the screw rod nut, the movable bridge seat is fixed on the screw rod nut, the top end of the first piston rod is connected with the movable bridge seat, the screw rod is fixed on the first driving motor 5, and the first driving motor is fixed on the outer shell of the mechanical part. The screw rod and the piston rod are designed to be parallel to the same plane. The movable bridge seat is provided with a first light blocking sheet 26, and the original point position, namely the liquid suction starting position, is determined so as to ensure that the zero point starting position of liquid suction is consistent every time, and the first light blocking sheet is installed towards the direction of the pump body.
The second transfer pump is driven by a screw rod assembly, which comprises a screw rod and a screw rod nut, wherein the screw rod is connected with the screw rod nut, the top end of a piston rod is connected with the screw rod nut, and the screw rod is fixed on the second driving motor 8.
The gun head adapter 4 is a through hole at the central position, the bottom comprises two sections of spherical bulges, the bottommost part is a first section of spherical bulge, the second section of bulge is larger than the first section of bulge, the top end of the gun head adapter is provided with a limiting ring, the outer diameter of the limiting ring is larger than the inner diameter of the adapter sleeve assembly, the outer diameter of the limiting ring is not larger than 9mm, the gun head adapter is the prior art in the field, and the description is not repeated here.
The adapter sleeve assembly comprises a jacking sleeve, a spring, a push rod 24 and a second light blocking piece 11, wherein the jacking sleeve is connected with the push rod, and the longitudinal central axis of the push rod is perpendicular to the transverse central line of the movable bridge seat and is in the same plane. The joint of the jacking sleeve and the ejector rod is provided with a spring, the spring sleeve and the ejector rod are respectively abutted against the joint of the jacking sleeve and the ejector rod and the equal-length limiting part of the spring at two ends, the light barrier is arranged at one end far away from the ejector rod and parallel to the ejector rod, and the adapter sleeve is the prior art in the field and is not repeated here.
The sensing device comprises a displacement original point sensor, a reset sensor, a pressure sensor and a capacitance sensor, wherein the displacement original point sensor is designed and arranged inside the mechanical shell, is arranged between the movable bridge seat 7 and the ejector rod 24 and is close to the top end of the ejector rod, and the starting position of the piston rod is determined to be at the bottom end, namely, liquid suction starts from the starting position every time. The pressure sensor is arranged in an external connecting hole of the cylinder body base. The reset sensor is designed and installed inside the mechanical shell, is far away from one end of the ejector rod and is parallel to the ejector rod. The capacitive sensor is arranged at the joint of the gun head adapter and the cylinder body base, and the sensors are all connected with the control module through circuits.
The first air pump device and the second air pump device of the embodiment are combined with the driving mechanism to meet the requirement of self-adaptive large-scale volume high-precision pipetting. The first air pump device is responsible for transferring small-volume (10-1000 ul) liquid, and the second air pump and the first air pump are jointly responsible for transferring large-volume liquid. The diameter of the closed cavity of the pump body component of the first air pump is small and long, so that the requirement of high flux on the small size of the liquid transfer pump is met while the liquid transfer precision of a small and medium size is ensured. The diameter of the second liquid-transferring pump closed cavity of the second air pump is used for carrying out length and diameter matching according to the requirement of liquid-transferring volume and the equipment installation requirement (the formula is V=pi×R 2 X L). The first air pump is connected with the second air pump through the air pipe, and the second air pump can be fixed at a certain position due to the fact that the air pipe is made of soft materials, and only the pump body assembly mechanism is emptiedThe three-dimensional movement in the room ensures the requirements of high flux of the full-automatic liquid workstation on small volume, light weight and accurate movement of the mechanical arm.
The adapter sleeve assembly 10 is used for realizing automatic gun head detachment, and the motor provides a force to enable the adapter sleeve to move so as to extrude the gun head out of the gun head adapter, thereby realizing automatic gun head detachment by liquid transfer and fully automating the liquid workstation.
The sensor is used for realizing original reset of the piston rod, position change of the adapter sleeve and pressure change induction, and the original reset of the piston rod is to provide signals for the control module through the sensor to acquire whether the piston rod is at the original position, so that the starting position of the movement of the piston rod is consistent every time, and whether the operation of the driving mechanism is normal or not is detected. The change in adapter sleeve position is also signaled by the sensor to the control module to capture the change in adapter sleeve position. The adapter sleeve squeezes the spring mechanism upward as the gun head is being retrieved, providing a change in position signal to determine that the gun head has been retrieved, and moves the spring mechanism downward as the gun head is being removed, providing a change in position signal to determine that the gun head has been removed. The pressure sensor probe is used for sealing the pressure sensor probe in the closed cavity to sense the pressure value. The change in the potential of the conductive tip and the conductive mesa of the instrument provides a signal to determine the change in the position of the conductive tip.
The application of the pipetting device in pipetting IVD samples comprises a small-volume pipetting mode and a large-volume pipetting mode, wherein the system operates in the small-volume pipetting mode under the liquid quantity of 10-1000 ul; and when the volume of the liquid exceeds 1000ul, the liquid is transferred into a large-volume liquid transferring mode, and the small volume is transferred into the large volume by adopting the combination of self-adaptive gas-liquid conversion.
According to the pipetting method of the pipetting device, a large-scale and high-precision pipetting is completed by adopting a pump, the pipetting method comprises small-volume pipetting and large-volume pipetting, the process of small-volume pipetting adopts full-automatic control, the pipetting amount is 10-1000ul, a pressure sensor is in circuit communication with a control module, a signal is provided for the control module through the sensor, whether a piston rod is at an origin position is obtained, and therefore the starting position of the piston rod movement is consistent every time, whether the operation of a driving mechanism is normal is detected, and the change of the position of an adapter sleeve is obtained: when the gun head is taken out, the adapter sleeve extrudes the elastic mechanism to move upwards, position signal change is provided to determine that the gun head is taken out, when the gun head is taken out, the adapter sleeve downwards moves the elastic mechanism to restore the original position, position signal change is provided to determine that the gun head is taken out, and air pressure change is sealed in the closed cavity through the pressure sensor probe to sense an air pressure value; the process of large-volume pipetting adopts full-automatic control, when the large-volume liquid is required to be sucked and discharged by more than 1000ul, the first piston rod continuously moves to the position where the second through hole of the piston rod coincides with the transverse through hole, meanwhile, the pressure sensor senses the pressure change, the first piston stops moving, and the second pipetting pump is synchronously started to move so as to provide the pressure for sucking and discharging the large-volume liquid.
The automatic induction of the gun head of the adapter is that the sensor provides the controller with the change of the position signal, thereby realizing the judgment of the gun head taking-off and carrying out error alarm. The automatic gun removing head is to provide signal software instructions to control the motor to operate the gun removing head through the sensor. The gun head disengaging state is set, and the original position of the adapter sleeve is signal 1. When the gun head is picked up, the adapter sleeve presses the elastic mechanism, and the sensor provides a position signal 2 to the control module, and then the gun head is determined to be picked up. When the control module receives a gun head removing command and sends a driving motor operation command in combination with the position signal 2, the driving motor provides a push rod acting force, so that the push-up sleeve is displaced, the gun head is separated from the gun head adapter, the adapter sleeve elastic mechanism returns to an original state, the sensor provides the position signal 1 for the control module, so that the gun head is judged to be separated, and the pipette gun head adapter and the control module can be realized by adopting conventional means in the field and are not repeated.
The device adopts the parallel structure of the screw rod and the piston rod, avoids operation instability caused by overlong same axis while ensuring enough travel, and ensures that the length and width of the design structure more reasonably meet the requirement of an automatic liquid platform on the three-dimensional movement of the liquid transferring pump space.
The modularized design has the advantages that the control module and the mechanical module are integrated into a whole independently, the structure is more flexible, the assembly is more stable, and the assembly is simpler. Greatly improves the diversified expansion and simultaneously saves the assembly cost and the maintenance cost.
The invention solves the problems of low automation degree, poor compatibility and high cost of the large-volume liquid workstation and the limitation of the prior commercial liquid transferring pump device.
The foregoing description is only of the preferred embodiments of the present invention, and is not intended to limit the scope of the invention, but rather is intended to cover any equivalents of the structures or equivalent processes disclosed herein or in the alternative, which may be employed directly or indirectly in other related arts.
Claims (10)
1. The utility model provides a pipetting device, its characterized in that includes first pipetting pump, second pipetting pump and induction system, and less volume liquid is absorbed and discharged to first pipetting pump completion, and the absorption and the discharge of more volume liquid are accomplished to the second pipetting pump, and first pipetting pump is provided with the biggest volume, and biggest volume department sets up the passageway and communicates with the second pipetting pump, induction system intercommunication first pipetting pump's pipetting channel, detects the pressure in the pipetting channel comes the stop of control first pipetting pump and the operation of synchronous start second pipetting pump.
2. The pipetting device of claim 1, wherein the first pipetting pump comprises a first cylinder, a piston rod and a first drive mechanism, the piston rod is movably disposed in the first cylinder under the drive of the first drive mechanism, a first through hole is disposed in the center of the bottom of the piston rod, a second through hole is disposed at an end of the piston rod near the bottom, and the first through hole is in communication with the second through hole.
3. The pipetting device of claim 2 wherein the first cylinder includes a cylinder base, a gas circuit adapter, a cylinder head, the cylinder base, the gas circuit adapter, and a cylinder head concentric axis through bore, the piston rod reciprocating within the cylinder base, the gas circuit adapter, and the cylinder head concentric axis through bore.
4. The pipetting device of claim 3, wherein the sensing device comprises a pressure sensor disposed on one side of the bottom of the cylinder base, the pressure sensor being in communication with the cylinder base, the gas circuit adapter, and the cylinder head concentric axis through hole via a pipeline.
5. The pipetting device of claim 4, wherein the cylinder base upper portion and the cylinder cover lower portion are cylindrical convex structures, the two ends of the air passage adapter are cylindrical concave structures, the concave structures and the convex structures are concentric with each other and cooperate to form the first cylinder, the length of the convex structures is smaller than the depth of the concave structures, and the connection parts of the air passage adapter, the cylinder base and the cylinder cover are respectively provided with a first sealing ring, a second sealing ring, a third sealing ring and a fourth sealing ring.
6. The pipetting device of claim 5 wherein the air passage adapter has a transverse through hole at a transverse center thereof at a maximum volume of the first pipetting pump, a longitudinal through hole is provided on one side of the air passage adapter, the longitudinal through hole communicates with the transverse through hole, the piston rod reaches the maximum volume, and the second through hole communicates with the transverse through hole.
7. The pipetting device of claim 6, wherein the second pipetting device comprises a pipeline, a second cylinder, a second piston rod and a second driving mechanism, one end of the pipeline is communicated with the longitudinal through hole, the other end of the pipeline is communicated with the second cylinder, a communication channel of the first pipetting device and the second pipetting device is formed, and the second piston rod is driven by the second driving mechanism to reciprocate in the second cylinder to suck and discharge liquid.
8. The pipetting device of claim 6, wherein the pipetting device comprises a housing comprising a first housing and a second housing, the second housing being connected to the top of the first housing, the first housing being a mechanical part housing, the second housing being a control part housing, the bottom of the first housing being provided with a through hole slot in communication with the first cylinder, the through hole slot being provided with a pipette tip adapter for pipetting.
9. A pipetting device as recited in any one of claims 1-8 wherein the use of the pipetting device in processing IVD sample pipetting comprises a small volume pipetting mode, a large volume pipetting mode, and a system operating in a small volume pipetting mode at a liquid level of 10-1000 ul; and when the volume of the liquid exceeds 1000ul, the liquid is transferred into a large-volume liquid transferring mode, and the small volume is transferred into the large volume by adopting the combination of self-adaptive gas-liquid conversion.
10. A pipetting device as recited in any one of claims 1-8 wherein the pipetting method of the pipetting device comprises the steps of: the method comprises the steps of adopting a pump to finish large-scale and high-precision pipetting, including small-volume pipetting and large-volume pipetting, adopting full-automatic control in the process of small-volume pipetting, wherein the pipetting amount is 10-1000ul, a sensing device is in circuit communication with a control module, a sensor in the sensing device is used for providing signals for the control module to acquire whether a piston rod is at an origin position, so that the starting position of the piston rod movement is consistent every time, whether a driving mechanism operates normally is detected, and the change of the position of an adapter sleeve is acquired, and the air pressure change is sealed in a sealed cavity through a pressure sensor probe to sense an air pressure value; the process of large-volume pipetting adopts full automatic control, when the large-volume liquid of more than 1000uL is required to be sucked and discharged, the first piston rod continuously moves to the position where the second through hole of the piston rod coincides with the transverse through hole, meanwhile, the pressure sensor senses the pressure change, the first piston rod stops moving, and the second pipetting pump is synchronously started to move so as to provide the pressure for sucking and discharging the large-volume liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310883097.4A CN117019253A (en) | 2023-07-18 | 2023-07-18 | Liquid transferring pump device and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310883097.4A CN117019253A (en) | 2023-07-18 | 2023-07-18 | Liquid transferring pump device and use method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117019253A true CN117019253A (en) | 2023-11-10 |
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ID=88628894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310883097.4A Pending CN117019253A (en) | 2023-07-18 | 2023-07-18 | Liquid transferring pump device and use method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117019253A (en) |
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2023
- 2023-07-18 CN CN202310883097.4A patent/CN117019253A/en active Pending
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