CN117017833A - Compound with multiple-effect repairing and anti-aging effects and preparation method thereof - Google Patents
Compound with multiple-effect repairing and anti-aging effects and preparation method thereof Download PDFInfo
- Publication number
- CN117017833A CN117017833A CN202311158649.1A CN202311158649A CN117017833A CN 117017833 A CN117017833 A CN 117017833A CN 202311158649 A CN202311158649 A CN 202311158649A CN 117017833 A CN117017833 A CN 117017833A
- Authority
- CN
- China
- Prior art keywords
- freeze
- dried powder
- compound
- water
- aging effects
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000003712 anti-aging effect Effects 0.000 title claims abstract description 32
- 150000001875 compounds Chemical class 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 239000000843 powder Substances 0.000 claims abstract description 68
- 239000002904 solvent Substances 0.000 claims abstract description 55
- 229920001296 polysiloxane Polymers 0.000 claims abstract description 37
- -1 polysiloxane Polymers 0.000 claims abstract description 37
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000007788 liquid Substances 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 18
- 125000000129 anionic group Chemical group 0.000 claims abstract description 15
- XMWRBQBLMFGWIX-UHFFFAOYSA-N C60 fullerene Chemical compound C12=C3C(C4=C56)=C7C8=C5C5=C9C%10=C6C6=C4C1=C1C4=C6C6=C%10C%10=C9C9=C%11C5=C8C5=C8C7=C3C3=C7C2=C1C1=C2C4=C6C4=C%10C6=C9C9=C%11C5=C5C8=C3C3=C7C1=C1C2=C4C6=C2C9=C5C3=C12 XMWRBQBLMFGWIX-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229910003472 fullerene Inorganic materials 0.000 claims abstract description 13
- ROTFCACGLKOUGI-JYJNAYRXSA-N (2s)-2-[[(2s)-2-[[(2s)-2-(3-acetamidopropanoylamino)-3-(1h-imidazol-5-yl)propanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1h-imidazol-5-yl)propanoic acid Chemical compound C([C@H](NC(=O)CCNC(=O)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC=1NC=NC=1)C(O)=O)C1=CN=CN1 ROTFCACGLKOUGI-JYJNAYRXSA-N 0.000 claims abstract description 11
- 229920002385 Sodium hyaluronate Polymers 0.000 claims abstract description 11
- 239000000284 extract Substances 0.000 claims abstract description 11
- 229940010747 sodium hyaluronate Drugs 0.000 claims abstract description 11
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 11
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 claims abstract description 10
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims abstract description 10
- 229930195725 Mannitol Natural products 0.000 claims abstract description 10
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 10
- 239000001168 astaxanthin Substances 0.000 claims abstract description 10
- 235000013793 astaxanthin Nutrition 0.000 claims abstract description 10
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 claims abstract description 10
- 229940022405 astaxanthin Drugs 0.000 claims abstract description 10
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000000594 mannitol Substances 0.000 claims abstract description 10
- 235000010355 mannitol Nutrition 0.000 claims abstract description 10
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 10
- 229940046009 vitamin E Drugs 0.000 claims abstract description 10
- 239000011709 vitamin E Substances 0.000 claims abstract description 10
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims abstract description 9
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims abstract description 9
- 241001506047 Tremella Species 0.000 claims abstract description 8
- 150000004676 glycans Chemical class 0.000 claims abstract description 8
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 8
- 239000005017 polysaccharide Substances 0.000 claims abstract description 8
- 244000288157 Passiflora edulis Species 0.000 claims abstract description 6
- 235000000370 Passiflora edulis Nutrition 0.000 claims abstract description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-O ammonium group Chemical class [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims abstract description 6
- 229940059958 centella asiatica extract Drugs 0.000 claims abstract description 6
- 229960001855 mannitol Drugs 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 58
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 28
- 239000001257 hydrogen Substances 0.000 claims description 28
- 229910052739 hydrogen Inorganic materials 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 21
- 238000003756 stirring Methods 0.000 claims description 21
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 claims description 19
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 19
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 19
- 229920002545 silicone oil Polymers 0.000 claims description 16
- 239000011259 mixed solution Substances 0.000 claims description 14
- 239000007864 aqueous solution Substances 0.000 claims description 11
- FPYJFEHAWHCUMM-UHFFFAOYSA-N maleic anhydride Chemical compound O=C1OC(=O)C=C1 FPYJFEHAWHCUMM-UHFFFAOYSA-N 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 8
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 7
- 239000003377 acid catalyst Substances 0.000 claims description 6
- 125000003342 alkenyl group Chemical group 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 238000001291 vacuum drying Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 27
- 230000002087 whitening effect Effects 0.000 abstract description 13
- 230000003020 moisturizing effect Effects 0.000 abstract description 9
- 230000002195 synergetic effect Effects 0.000 abstract description 8
- 230000000052 comparative effect Effects 0.000 description 58
- 239000000523 sample Substances 0.000 description 26
- 238000012360 testing method Methods 0.000 description 23
- 108010003272 Hyaluronate lyase Proteins 0.000 description 15
- 102000001974 Hyaluronidases Human genes 0.000 description 15
- 229960002773 hyaluronidase Drugs 0.000 description 15
- 230000014759 maintenance of location Effects 0.000 description 15
- 238000004090 dissolution Methods 0.000 description 12
- 239000000047 product Substances 0.000 description 12
- 235000011187 glycerol Nutrition 0.000 description 10
- 239000008176 lyophilized powder Substances 0.000 description 10
- 108090000765 processed proteins & peptides Proteins 0.000 description 9
- 230000005764 inhibitory process Effects 0.000 description 8
- 239000002245 particle Substances 0.000 description 8
- 239000006185 dispersion Substances 0.000 description 7
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 5
- 239000004480 active ingredient Substances 0.000 description 5
- 229920002674 hyaluronan Polymers 0.000 description 5
- 229960003160 hyaluronic acid Drugs 0.000 description 5
- 230000035755 proliferation Effects 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000036564 melanin content Effects 0.000 description 4
- 229920001184 polypeptide Polymers 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 3
- 239000008351 acetate buffer Substances 0.000 description 3
- 239000002390 adhesive tape Substances 0.000 description 3
- 150000001450 anions Chemical class 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- WCDDVEOXEIYWFB-VXORFPGASA-N (2s,3s,4r,5r,6r)-3-[(2s,3r,5s,6r)-3-acetamido-5-hydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4,5,6-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@@H]1C[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O)[C@H](O)[C@H]1O WCDDVEOXEIYWFB-VXORFPGASA-N 0.000 description 2
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 description 2
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 2
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 2
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- 230000003266 anti-allergic effect Effects 0.000 description 2
- 239000003125 aqueous solvent Substances 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229940014041 hyaluronate Drugs 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 229950006780 n-acetylglucosamine Drugs 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 210000001626 skin fibroblast Anatomy 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- RRJOMESUBQAYOA-BZSNNMDCSA-N (2S)-2-[[(2S)-2-[[2-[[(2S)-2-acetamido-6-aminohexanoyl]amino]acetyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-6-aminohexanamide Chemical group NCCCC[C@H](NC(=O)C)C(=O)NCC(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(N)=O)CC1=CN=CN1 RRJOMESUBQAYOA-BZSNNMDCSA-N 0.000 description 1
- ITIMHIATVYROGF-XWUOBKMESA-N (3S)-3-[[(2S)-2-acetamido-6-aminohexanoyl]amino]-4-[[(2S)-1-[[(1S)-1-carboxy-2-(4-hydroxyphenyl)ethyl]amino]-3-methyl-1-oxobutan-2-yl]amino]-4-oxobutanoic acid Chemical group CC(C)[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCCN)NC(C)=O)C(=O)N[C@@H](Cc1ccc(O)cc1)C(O)=O ITIMHIATVYROGF-XWUOBKMESA-N 0.000 description 1
- 229940015975 1,2-hexanediol Drugs 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 101100004286 Caenorhabditis elegans best-5 gene Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000016942 Elastin Human genes 0.000 description 1
- 108010014258 Elastin Proteins 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- BIVBRWYINDPWKA-VLQRKCJKSA-L Glycyrrhizinate dipotassium Chemical compound [K+].[K+].O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@H]1CC[C@]2(C)[C@H]3C(=O)C=C4[C@@H]5C[C@](C)(CC[C@@]5(CC[C@@]4(C)[C@]3(C)CC[C@H]2C1(C)C)C)C(O)=O)C([O-])=O)[C@@H]1O[C@H](C([O-])=O)[C@@H](O)[C@H](O)[C@H]1O BIVBRWYINDPWKA-VLQRKCJKSA-L 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 238000000134 MTT assay Methods 0.000 description 1
- 231100000002 MTT assay Toxicity 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000001153 anti-wrinkle effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229940101029 dipotassium glycyrrhizinate Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 229920002549 elastin Polymers 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 description 1
- 229940087603 grape seed extract Drugs 0.000 description 1
- 235000002532 grape seed extract Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- FHKSXSQHXQEMOK-UHFFFAOYSA-N hexane-1,2-diol Chemical compound CCCCC(O)CO FHKSXSQHXQEMOK-UHFFFAOYSA-N 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000037307 sensitive skin Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 230000037067 skin hydration Effects 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 125000000647 trehalose group Chemical group 0.000 description 1
- 239000001717 vitis vinifera seed extract Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/89—Polysiloxanes
- A61K8/891—Polysiloxanes saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0216—Solid or semisolid forms
- A61K8/022—Powders; Compacted Powders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/731—Cellulose; Quaternized cellulose derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Emergency Medicine (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention belongs to the technical field of skin care products, and discloses a compound with multiple-effect repairing and anti-aging effects and a preparation method thereof. The compound comprises freeze-dried powder and solvent liquid matched with the freeze-dried powder for use; the freeze-dried powder comprises astaxanthin, vitamin E, mannitol, sodium hyaluronate, acetyl tetrapeptide-5, fullerene, polyquaternary ammonium salt-10 and glyceroglycolipid; the solvent liquid comprises water, glycerol, butanediol, centella asiatica extract, passion fruit extract, tremella polysaccharide, water-soluble polysiloxane film forming agent and disodium hydrogen phosphate. The compound has synergistic whitening, anti-aging and repairing effects through the addition of functional active components in the freeze-dried powder and the solvent. And further, the anionic water-soluble polysiloxane with a specific structure is used as a film forming agent, so that the skin feel of the product can be obviously improved, and the moisturizing effect of the product can be improved.
Description
Technical Field
The invention belongs to the technical field of skin care products, and particularly relates to a compound with multiple-effect repairing and anti-aging effects and a preparation method thereof.
Background
With the improvement of living standard, consumers are paying more attention to the ingredients and efficacy of cosmetic formulations. However, highly active ingredients are often unstable in physicochemical properties, and particularly lose or lose activity very easily under normal temperature and liquid conditions. Because the vacuum freeze-drying technology is carried out in a low-temperature and low-oxygen environment, most biological reactions are stagnant, liquid water is not present in the treatment process, and moisture is directly sublimated in a solid state, so that the damage to the structure and the characteristics of tissues and cells is small, the original structure and the shape of the materials are protected to the greatest extent, meanwhile, the freeze-drying technology effectively retains the original characteristics of components, particularly improves the stability of a plurality of thermosensitive active components, protects and retains the activities of some higher biological activity or easily-oxidized components, and finally can obtain a high-quality dried product with both appearance and internal quality. The color of the freeze-dried powder product is not changed basically after drying, the morphological structure is loose, and the freeze-dried powder product can be quickly dissolved and restored to a solution form after being added with solvents such as water and the like, and the freeze-dried powder product presents the original physicochemical property and biological activity. In addition, in view of the preparation principle of the freeze-dried powder, the moisture content in the freeze-dried powder is very low, so that the stability of the product is greatly improved, the transportation is convenient, and the storage life of the product is prolonged.
CN 114259418A discloses a polypeptide lyophilized powder comprising a peptide and an excipient to which the peptide is easily attached; the peptide comprises 0.1-2 parts by weight of oligopeptide-1, 0.1-2 parts by weight of tripeptide-1 and 0.1-2 parts by weight of oligopeptide-1; the excipient comprises mannitol 4-25 weight parts, disodium hydrogen phosphate 0.5-1.0 weight parts, and sodium dihydrogen phosphate 0.6-1.2 weight parts. The polypeptide freeze-dried powder preparation can be prepared by compounding the freeze-dried powder and solvent liquid. The method effectively protects the stability of the effective components of a plurality of thermosensitive biological products. But the freeze-dried powder adopts single polypeptide component, the used oligopeptide-1 has safety risk, and the development of the freeze-dried powder and the compound preparation with safer and more comprehensive efficacy has good market prospect.
CN 113171318A discloses a freeze-dried powder preparation with multiple effects of moisturizing, whitening, relieving and repairing. The raw materials of the freeze-dried powder preparation comprise the following components: mannitol, dipotassium glycyrrhizinate, cell complex biological peptide, tripeptide-1 copper, nicotinamide, glycyrrhiza glabra root extract and grape seed extract. The lyophilized powder preparation has four-in-one effects of moisturizing, whitening, relieving and repairing.
Due to the morphological characteristics of the freeze-dried powder, the freeze-dried powder is matched with a solvent liquid for use in application, and the solvent liquid plays a key role in the performance of the functional components of the freeze-dried powder. Patent CN 115212133A discloses a freeze-dried powder solvent, which is prepared from water, glycerol, propylene glycol, 1, 2-hexanediol, p-hydroxyacetophenone, butanediol, saccharide isomers, trehalose and oat peptide. The freeze-dried powder solvent is matched with each other through various components, and has synergistic effect, excellent moisturizing and anti-wrinkle effects, the skin hydration capacity is improved, the elastin generation is promoted, and cells are rich in elasticity; the skin antioxidation capability can be improved, melanin precipitation is effectively prevented, the skin is kept bright, and the health and freshness of the skin are maintained; improving the repairing effect of skin.
The existing freeze-dried powder and solvent liquid components generally pay attention to whether the efficacy is comprehensive or not, so active ingredients with high content such as repairing and whitening are used, however, the active ingredients with the efficacy are generally components such as polypeptide, hyaluronic acid, plant extracts, fullerene and the like, the defect of poor film forming spreadability exists, and the skin is easy to feel tight when the freeze-dried powder and solvent liquid are applied to the skin. How to improve the skin feel of the freeze-dried powder and the compound preparation when in use is a technical problem which needs to be solved by the technicians in the field.
Disclosure of Invention
In view of the above drawbacks and shortcomings of the prior art, a primary object of the present invention is to provide a composite with multiple-effect repair and anti-aging effects. The compound has synergistic whitening, anti-aging and repairing effects through the addition of various functional active components, and improves the skin feel through the specific water-soluble polysiloxane film forming agent.
Another object of the present invention is to provide a method for preparing the above-mentioned complex having multiple-effect repairing and anti-aging effects.
The invention aims at realizing the following technical scheme:
a compound with multiple-effect repairing and anti-aging effects comprises lyophilized powder and solvent solution matched with the lyophilized powder; the freeze-dried powder comprises astaxanthin, vitamin E, mannitol, sodium hyaluronate, acetyl tetrapeptide-5, fullerene, polyquaternary ammonium salt-10 and glyceroglycolipid; the solvent liquid comprises water, glycerol, butanediol, centella asiatica extract, passion fruit extract, tremella polysaccharide, water-soluble polysiloxane film forming agent and disodium hydrogen phosphate.
Further, the water-soluble polysiloxane film forming agent is water-soluble polysiloxane with an anionic structure, and is prepared by the following method:
adding hydrogen-containing silicone oil and allyl polyoxyethylene ether into an alcohol solvent to be uniformly dissolved, heating to 70-85 ℃, adding a chloroplatinic acid catalyst, stirring and reacting until the alkenyl reaction is complete, adding maleic anhydride, continuously carrying out heat preservation and reacting for 0.5-2 h, and vacuum drying to remove the solvent, thereby obtaining the water-soluble polysiloxane with the anionic structure.
Further preferably, the hydrogen-containing silicone oil is a hydrogen-containing silicone oil having a (active) hydrogen content of 0.1% to 0.9% and a viscosity of 50 to 500mpa.s at 25 ℃.
Further preferably, the molecular weight of the allyl polyoxyethylene ether is 200 to 2000.
Further preferably, the addition amount of the allyl polyoxyethylene ether is 0.05 to 0.5 times of the molar amount of hydrogen contained in the hydrogen-containing silicone oil.
Further preferably, the addition amount of the maleic anhydride is 0.8 to 1 time of the molar amount of the allyl polyoxyethylene ether.
Further, the freeze-dried powder comprises the following raw material components in parts by weight:
further, the solvent liquid comprises the following raw material components in parts by weight:
further, in the compound with multiple-effect repairing and anti-aging effects, the ratio of the freeze-dried powder to the solvent is 50-200 mg:1-10 mL.
The preparation method of the compound with the multiple-effect repairing and anti-aging effects comprises the following preparation steps:
(1) Preparing freeze-dried powder: adding astaxanthin, vitamin E and glyceroglycolipid into ethanol, dissolving uniformly, adding fullerene, stirring and dispersing uniformly to obtain ethanol mixed solution; mannitol, sodium hyaluronate, acetyl tetrapeptide-5 and polyquaternium-10 are added into water to be dissolved uniformly, so as to obtain an aqueous solution; homogenizing and mixing the ethanol mixed solution and the aqueous solution, filtering to remove insoluble substances, and vacuum freeze-drying to obtain freeze-dried powder;
(2) Preparing solvent liquid: adding glycerol, butanediol and water-soluble polysiloxane film forming agent into water, stirring and dissolving uniformly, adding herba Centellae extract, herba Passiflorae Caeruleae fruit extract and tremella polysaccharide, stirring and dissolving uniformly, and finally adding disodium hydrogen phosphate and dissolving uniformly to obtain a vehicle solution;
(3) The freeze-dried powder and the solvent liquid are mixed according to a proportion, dissolved and dispersed uniformly to obtain the compound with multiple-effect repairing and anti-aging effects.
Further, in the preparation process of the freeze-dried powder, the mass ratio of the ethanol to the water is kept to be 1-3:1 when the ethanol mixed solution and the water solution are uniformly stirred and mixed.
Compared with the prior art, the invention has the beneficial effects that:
(1) The invention adopts anion water-soluble polysiloxane with a specific structure as a film forming agent, and can form a soft polysiloxane film with polyquaternium-10 through ion adsorption codeposition in the use process, thereby remarkably improving the use skin feel and improving the moisturizing effect of the product.
(2) The invention has synergistic whitening, anti-aging and repairing effects through the addition of functional active components in the freeze-dried powder and the solvent.
(3) The freeze-dried powder adopts the specific glyceroglycolipid as the carrier and the protective agent of the effective active ingredients, has obvious function of stabilizing the effective active ingredients, and can better synergistically improve the effects of moisturizing, repairing and relieving.
(4) The invention adopts the specific polyquaternium-10 as the forming auxiliary agent of the freeze-dried powder, and can reduce the particle size of the freeze-dried powder and obviously promote the dissolution and dispersion effects of the freeze-dried powder in aqueous solvent by controlling the ratio of solvent ethanol to water in the preparation process of the freeze-dried powder.
Detailed Description
The present invention will be described in further detail with reference to examples, but embodiments of the present invention are not limited thereto.
Example 1
The compound with multiple-effect repairing and anti-aging effects comprises freeze-dried powder and solvent liquid matched with the freeze-dried powder.
The freeze-dried powder is prepared by the following method:
(1) According to the mass parts, 4 parts of astaxanthin, 6 parts of vitamin E and 20 parts of glyceroglycolipid are added into 100 parts of ethanol for uniform dissolution, and then 3 parts of fullerene is added for uniform stirring and dispersion, thus obtaining ethanol mixed solution.
(2) 8 parts of mannitol, 2 parts of sodium hyaluronate, 5 parts of acetyl tetrapeptide-5 and 3 parts of polyquaternary ammonium salt-10 are added into 50 parts of water to be uniformly dissolved, so as to obtain an aqueous solution.
(3) And (3) homogenizing and mixing the ethanol mixed solution in the step (1) and the aqueous solution in the step (2), filtering to remove insoluble substances, and performing vacuum freeze drying to obtain the whitening compact repairing freeze-dried powder.
The solvent liquid is prepared by the following method:
according to the mass parts, adding 4 parts of glycerin, 3 parts of butanediol and 2 parts of water-soluble polysiloxane film forming agent into 86.8 parts of water, stirring and dissolving uniformly, adding 1.5 parts of centella asiatica extract, 1.5 parts of passion fruit extract and 1 part of tremella polysaccharide, stirring and dissolving uniformly, and finally adding 0.2 part of disodium hydrogen phosphate, dissolving uniformly, thus obtaining the solvent.
The water-soluble polysiloxane film forming agent is water-soluble polysiloxane with an anionic structure, and is prepared by the following method:
adding hydrogen-containing silicone oil with hydrogen content of 0.45% and viscosity of 150mPa.s at 25 ℃ and allyl polyoxyethylene ether with average molecular weight of 800 into ethanol solvent, and dissolving uniformly, wherein the addition amount of the allyl polyoxyethylene ether is 0.3 times of the molar weight of hydrogen contained in the hydrogen-containing silicone oil. Then heating to 75-80 ℃, adding chloroplatinic acid catalyst, stirring and reacting until the alkenyl reaction is complete, adding maleic anhydride, continuously preserving heat and reacting for 2 hours, wherein the adding amount of the maleic anhydride is 1 time of the molar amount of the allyl polyoxyethylene ether, and vacuum drying to remove the solvent, thus obtaining the water-soluble polysiloxane with the anionic structure.
When in use, the freeze-dried powder and the solvent liquid are mixed, dissolved and uniformly dispersed according to the proportion of 100mg to 5mL, so as to obtain the compound with multiple-effect repairing and anti-aging effects.
In the embodiment, the mass ratio of the mixed solution solvent ethanol to water before vacuum freeze drying in the preparation process of the freeze-dried powder is respectively regulated to be 0.5:1, 1:1, 2:1, 3:1 and 4:1, and the total addition amount of the ethanol and the water is 150 parts by weight and is kept unchanged. The average particle diameter and the dissolution time (the time from shaking table mixing to complete dissolution and dispersion uniformity) of the obtained lyophilized powder were respectively measured, and the results are shown in table 1 below.
TABLE 1
| Test sample | Average particle diameter | Dissolution time |
| 0.5:1 | 465.4nm | 1min43s |
| 1:1 | 245.3nm | 56s |
| 2:1 | 158.6nm | 45s |
| 3:1 | 212.7nm | 51s |
| 4:1 | 328.2nm | 1min15s |
As shown in table 1, the particle size of the obtained lyophilized powder tended to decrease and increase with increasing mass ratio of ethanol to water in the mixed solution solvent before lyophilization, and the smaller the particle size was, the shorter the dissolution time was. Under the condition that the mass ratio of the ethanol to the water is 1-3:1, the dissolution and dispersion time of the obtained freeze-dried powder in the solvent liquid can be controlled within 1 min.
Example 2
The compound with multiple-effect repairing and anti-aging effects comprises freeze-dried powder and solvent liquid matched with the freeze-dried powder.
The freeze-dried powder is prepared by the following method:
(1) According to the mass parts, 2 parts of astaxanthin, 8 parts of vitamin E and 15 parts of glyceroglycolipid are added into 80 parts of ethanol for uniform dissolution, and then 2 parts of fullerene is added for uniform stirring and dispersion, thus obtaining ethanol mixed solution.
(2) 5 parts of mannitol, 1 part of sodium hyaluronate, 6 parts of acetyl tetrapeptide-5 and 2 parts of polyquaternary ammonium salt-10 are added into 40 parts of water to be uniformly dissolved, so as to obtain an aqueous solution.
(3) And (3) homogenizing and mixing the ethanol mixed solution in the step (1) and the aqueous solution in the step (2), filtering to remove insoluble substances, and performing vacuum freeze drying to obtain the whitening compact repairing freeze-dried powder.
The solvent liquid is prepared by the following method:
according to the mass parts, adding 6 parts of glycerin, 1 part of butanediol and 0.5 part of water-soluble polysiloxane film forming agent into 82.1 parts of water, stirring and dissolving uniformly, adding 4 parts of centella asiatica extract, 4 parts of passion fruit extract and 2 parts of tremella polysaccharide, stirring and dissolving uniformly, and finally adding 0.4 part of disodium hydrogen phosphate, dissolving uniformly, thus obtaining the solvent.
The water-soluble polysiloxane film forming agent is water-soluble polysiloxane with an anionic structure, and is prepared by the following method:
adding hydrogen-containing silicone oil with hydrogen content of 0.55% and viscosity of 250mPa.s at 25 ℃ and allyl polyoxyethylene ether with average molecular weight of 1200 into ethanol solvent, and dissolving uniformly, wherein the addition amount of the allyl polyoxyethylene ether is 0.2 times of the molar weight of hydrogen contained in the hydrogen-containing silicone oil. Then heating to 75-80 ℃, adding chloroplatinic acid catalyst, stirring and reacting until the alkenyl reaction is complete, adding maleic anhydride, continuously preserving heat and reacting for 2 hours, wherein the adding amount of the maleic anhydride is 1 time of the molar amount of the allyl polyoxyethylene ether, and vacuum drying to remove the solvent, thus obtaining the water-soluble polysiloxane with the anionic structure.
When in use, the freeze-dried powder and the solvent liquid are mixed, dissolved and uniformly dispersed according to the proportion of 100mg to 3mL, so as to obtain the compound with multiple-effect repairing and anti-aging effects.
Example 3
The compound with multiple-effect repairing and anti-aging effects comprises freeze-dried powder and solvent liquid matched with the freeze-dried powder.
The freeze-dried powder is prepared by the following method:
(1) According to the mass parts, adding 6 parts of astaxanthin, 4 parts of vitamin E and 30 parts of glyceroglycolipid into 120 parts of ethanol for uniform dissolution, and then adding 4 parts of fullerene for uniform stirring and dispersion to obtain an ethanol mixed solution.
(2) 10 parts of mannitol, 3 parts of sodium hyaluronate, 3 parts of acetyl tetrapeptide-5 and 5 parts of polyquaternary ammonium salt-10 are added into 60 parts of water to be uniformly dissolved, so as to obtain an aqueous solution.
(3) And (3) homogenizing and mixing the ethanol mixed solution in the step (1) and the aqueous solution in the step (2), filtering to remove insoluble substances, and performing vacuum freeze drying to obtain the whitening compact repairing freeze-dried powder.
The solvent liquid is prepared by the following method:
according to the mass parts, adding 2 parts of glycerin, 5 parts of butanediol and 4 parts of water-soluble polysiloxane film forming agent into 86.4 parts of water, stirring and dissolving uniformly, adding 1 part of centella asiatica extract, 1 part of passion fruit extract and 0.5 part of tremella polysaccharide, stirring and dissolving uniformly, and finally adding 0.1 part of disodium hydrogen phosphate, dissolving uniformly, thus obtaining the solvent.
The water-soluble polysiloxane film forming agent is water-soluble polysiloxane with an anionic structure, and is prepared by the following method:
adding hydrogen-containing silicone oil with the hydrogen content of 0.36% and the viscosity of 200mPa.s at 25 ℃ and allyl polyoxyethylene ether with the average molecular weight of 1000 into an ethanol solvent, and uniformly dissolving, wherein the adding amount of the allyl polyoxyethylene ether is 0.4 times of the molar weight of hydrogen contained in the hydrogen-containing silicone oil. Then heating to 75-80 ℃, adding chloroplatinic acid catalyst, stirring and reacting until the alkenyl reaction is complete, adding maleic anhydride, continuously preserving heat and reacting for 2 hours, wherein the addition amount of the maleic anhydride is 0.9 times of the molar amount of the allyl polyoxyethylene ether, and vacuum drying to remove the solvent, thus obtaining the water-soluble polysiloxane with the anionic structure.
When in use, the freeze-dried powder and the solvent liquid are mixed, dissolved and uniformly dispersed according to the proportion of 100mg to 5mL, so as to obtain the compound with multiple-effect repairing and anti-aging effects.
Comparative example 1
In this comparative example, the preparation process of the lyophilized powder was not added with polyquaternium-10, and the rest was the same.
The lyophilized powders obtained in example 1 and comparative example were each tested for the average particle size and dissolution time in a solvent liquid, and the results are shown in table 2 below.
TABLE 2
| Test sample | Average particle diameter | Dissolution time |
| Example 1 | 158.6nm | 45s |
| Comparative example 1 | 652.7nm | 2min56s |
As can be seen from the results in table 2, the invention adopts the specific polyquaternium-10 as the forming auxiliary agent of the freeze-dried powder, which can reduce the particle size of the freeze-dried powder and obviously promote the dissolution and dispersion of the freeze-dried powder in the aqueous solvent.
Comparative example 2
In this comparative example, compared with example 1, no glyceroglycolipid was added during the preparation of the lyophilized powder, and the remaining components were the same.
Comparative example 3
Compared with the example 1, the freeze-dried powder adopts the same amount of trehalose to replace glyceroglycolipid in the preparation process, and the rest components are the same.
Comparative example 4
Compared with the example 1, the freeze-dried powder is prepared without adding astaxanthin and vitamin E, and the rest components are the same.
Comparative example 5
In this comparative example, sodium hyaluronate and acetyl tetrapeptide-5 were not added during the preparation of the lyophilized powder as in example 1, and the remaining components were the same.
Comparative example 6
Compared with the example 1, the comparative example has the advantages that fullerene is not added in the preparation process of the freeze-dried powder, and the rest components are the same.
Comparative example 7
In this comparative example, as compared with example 1, no water-soluble polysiloxane film former was added during the solvent liquid preparation, and the remaining components were the same.
Comparative example 8
Compared with the example 1, the comparative example adopts equivalent nonionic structure water-soluble polysiloxane to replace anionic structure water-soluble polysiloxane in the solvent liquid preparation process, and the rest components are the same. Compared with the preparation process of the water-soluble polysiloxane with the anion structure, the water-soluble polysiloxane with the nonionic structure is not added with maleic anhydride for anion modification, and the rest is the same, and the specific preparation process is as follows:
adding hydrogen-containing silicone oil with hydrogen content of 0.45% and viscosity of 150mPa.s at 25 ℃ and allyl polyoxyethylene ether with average molecular weight of 800 into ethanol solvent, and dissolving uniformly, wherein the addition amount of the allyl polyoxyethylene ether is 0.3 times of the molar weight of hydrogen contained in the hydrogen-containing silicone oil. Then heating to 75-80 ℃, adding chloroplatinic acid catalyst, stirring and reacting until the alkenyl reaction is complete, and vacuum drying to remove the solvent to obtain the nonionic structure water-soluble polysiloxane.
The composites obtained in the above examples and comparative examples were subjected to application performance tests:
1. skin feel test
55 female volunteers aged 25-40 years were selected and randomized into 11 groups of 5 persons each of examples 1-3 and comparative examples 1-8, respectively. The face of the subject is uniformly looped and smeared with 0.5g of sample to be measured, and the soft and moist skin feeling of the skin of the face after being smeared is scored, wherein the soft skin feeling is the best 5 minutes, the skin tightening feeling is the worst 1 minute, and the average value is taken, and the result is shown in the following table 3.
TABLE 3 Table 3
| Test sample | Skin feel score |
| Example 1 | 4.8 |
| Example 2 | 4.6 |
| Example 3 | 5.0 |
| Comparative example 1 | 4.2 |
| Comparative example 2 | 5.0 |
| Comparative example 3 | 4.6 |
| Comparative example 4 | 4.6 |
| Comparative example 5 | 4.6 |
| Comparative example 6 | 4.8 |
| Comparative example 7 | 3.2 |
| Comparative example 8 | 4.0 |
As can be seen from the results of comparative example 7 in table 3, the present invention can significantly improve the skin feel of the sample using the water-soluble polysiloxane with a specific anionic structure as the film forming agent, and it can be further found from the results of comparative example 1 and comparative example 8 that the polyquaternium-10 and the water-soluble polysiloxane with an anionic structure have a significant synergistic effect in improving the skin feel of the sample using, probably because the polyquaternium-10 and the water-soluble polysiloxane with an anionic structure are co-deposited into a film by ion adsorption to contribute to the improvement of the soft skin feel.
2. Moisture effect test
The testing method comprises the following steps:
under constant environmental conditions, a negative control group and a positive control group were set, the positive control group was 5% glycerol, the negative control group was deionized water, and the operation procedure was consistent with that of the sample group.
1. Placing color-changing silica gel at the bottom of the dryer, attaching 3M air-permeable adhesive tape onto glass plate, placing into the dryer one day in advance, and weighing M 0 ；
2. Accurately weighing the sample, and then weighing the sample by 2 mg+/-0.1 mg/cm according to the size of the adhesive tape 2 Weighing corresponding sample and reference substance, uniformly coating on adhesive tape, and weighing its weight M 1 ；
3. Placing the glass plate coated with the object to be measured into a dryer, and accurately weighing the mass M after 2 hours, 4 hours and 6 hours t ；
4. The formula for calculating the moisture retention (P) refers to the following formula:
moisture retention (%) = (M t –M 0 )/(M 1 –M 0 )×100％；
5. Relative moisture retention: comparison of the moisture retention properties of the test samples with 5% glycerol under the same conditions;
6. the relative moisture retention calculation formula is referred to below
Relative moisture retention (%) = (P) t –P 0 )/(P Glycerol –P 0 )×100％；
Wherein: pt-the moisture retention rate of the sample to be measured after t hours;
p0-moisture retention after t hours for negative control;
P glycerol Positive control% moisture retention after t hours.
The relative moisture retention rate evaluation results of the above examples and comparative examples are shown in table 4 below.
TABLE 4 Table 4
The relative moisture retention rate is lower than 0-50%, 50-100% is middle, 100-150% is high, and 150% is good. Tests show that the relative moisture retention rate of the sample solution in the embodiment of the invention is above 150% in 2 hours, and the moisture retention performance is good; the relative moisture retention rates of 4h and 6h are above 100%, and the moisture retention performance is high. Wherein the comparative example 2 and comparative example 3 were free of added glyceroglycolipid or replaced with trehalose, and their moisturizing properties were significantly reduced. It is explained that the improvement effect of the glyceroglycolipid on the moisturizing performance is most remarkable in the system of the present invention. In addition, as can be seen from the results of comparative example 7, the water-soluble silicone film-forming agent also has a more remarkable effect of improving the moisturizing performance.
3. Test of soothing efficacy
The current study considers the occurrence of sensitive skin as a complex process involving skin barrier-neurovascular-immune inflammation. Hyaluronic acid is the component with the largest content and proportion in the extracellular matrix, can maintain the volume of the extracellular matrix, regulate and control the secretion of cell growth factors and cytokines, and influence the adhesion, growth, proliferation and differentiation of cells, thereby playing an important role in maintaining the skin moisture and elasticity, wound healing, angiogenesis and other processes.
Hyaluronidase is an endogenous amino hexose which causes the decomposition of macromolecular hyaluronic acid, has strong correlation with inflammation and allergy, and researches report that various medicines for releasing histamine by fat large cells can regulate the activity of the hyaluronidase, and some antiallergic medicines have strong inhibition of the activity of the hyaluronidase, so that the inhibition of the activity of the hyaluronidase is used as an index for researching the antiallergic effect.
The macromolecular hyaluronic acid is decomposed to generate the N-acetylglucosamine under the action of hyaluronidase, and the activity of the hyaluronidase can be indirectly reacted by measuring the N-acetylglucosamine content in a reaction system. The inhibition of the activity of the hyaluronidase can ensure the normal content and function of the hyaluronic acid, so that whether a test sample has the effect of relieving or not can be judged by utilizing the inhibition rate of the hyaluronidase activity, and the higher the inhibition rate of the hyaluronidase activity is, the stronger the relieving effect of the substance is reflected, and the weaker the relieving effect is on the contrary.
The testing method comprises the following steps:
and (3) taking a proper amount of sample and a solution of hyaluronidase and the like for color reaction, testing absorbance at 530nm, and calculating the inhibition rate (%) of the hyaluronidase activity.
Hyaluronidase inhibition ratio (%) = [ (C-D) - (a-B) ]/(C-D) ×100%;
wherein: OD value of a- (hyaluronidase + sample + potassium hyaluronate) sample solution;
OD value of B- (hyaluronidase + sample + acetate buffer) sample blank;
OD value of C- (hyaluronidase + deionized water + potassium hyaluronate) control solution;
d- (acetate buffer + deionized water + acetate buffer) versus OD value of blank.
The results of the soothing efficacy evaluation of the above examples and comparative examples are shown in Table 5 below.
TABLE 5
| Test sample | Inhibition rate |
| Example 1 | 58.7％ |
| Example 2 | 60.6％ |
| Example 3 | 52.8％ |
| Comparative example 1 | 58.5％ |
| Comparative example 2 | 43.6％ |
| Comparative example 3 | 44.7％ |
| Comparative example 4 | 50.2％ |
| Comparative example 5 | 44.5％ |
| Comparative example 6 | 48.2％ |
| Comparative example 7 | 56.2％ |
| Comparative example 8 | 55.7％ |
As is apparent from the results in Table 5, the glyceroglycolipid of the present invention has a remarkable synergistic effect with sodium hyaluronate and acetyl tetrapeptide-5, and fullerene components in exerting the soothing effect of the product.
4. Whitening efficacy test
The testing method comprises the following steps: the 55 volunteers with darker complexion were screened and randomly divided into 11 groups of 5 volunteers. The skin red melanin tester and test probe (Mexameter MX18, CK, germany) were used to collect melanin MI data from the face prior to sample testing, and the data were used as skin background values. The sample was used once in the morning and evening by the test person, and data were collected after 4 weeks of continuous use. The change of the melanin content of the skin of the subject before and after the sample is tested is evaluated, so that the whitening effect is determined. The corresponding test results are shown in table 6 below.
Wherein, the melanin content change rate Δmi% = (T n -T 0 )/T 0 Averaging the final result; the higher Δmi% reduction value indicates better whitening efficacy.
Wherein T is n -the melanin content value of the tested area after 4 weeks of continuous use; t (T) 0 -initial value of melanin content of the test area.
TABLE 6
| Test sample | △MI％ |
| Example 1 | -16.78 |
| Example 2 | -16.52 |
| Example 3 | -15.51 |
| Comparative example 1 | -16.56 |
| Comparative example 2 | -9.23 |
| Comparative example 3 | -12.30 |
| Comparative example 4 | -7.98 |
| Comparative example 5 | -13.95 |
| Comparative example 6 | -12.24 |
| Comparative example 7 | -14.62 |
| Comparative example 8 | -15.81 |
As apparent from the results in Table 6, the glyceroglycolipid, astaxanthin, vitamin E and fullerene components of the present invention have obvious synergistic effect in the effect of whitening the product.
5. Test of repair and anti-aging effects
The test method employs a method of promoting human skin fibroblast proliferation (MTT assay):
taking human skin fibroblast in logarithmic growth phase, digesting, preparing into 1×10 concentration with DMEM medium containing 15% fetal calf serum and 1% diabody 5 Cell suspension/mL, inoculated in 96-well plate at 150. Mu.L per well, 37℃and 5% CO 2 After culturing in an incubator for 24 hours, the culture medium is replaced by DMEM culture medium containing 50 mug/mL of sample to be tested, 15% fetal bovine serum and 1% double antibody for continuous culture, after continuous culture for 24 hours, the culture medium is sucked, PBS is used for washing, 150 mug of DMEM culture medium containing 15% fetal bovine serum and 1% double antibody and 20 mug LMTT solution (5 mg/mL) are added, 37 ℃ and 5% CO are added 2 Culturing for 4h, absorbing supernatant, adding 150 μl of DMSO into each well, shaking for 10min, and dissolving the crystals completely. Meanwhile, a control group is arranged, wherein the control group is to replace a sample to be tested with normal saline, and the rest is identical, and each group has 5 compound holes. Absorbance was measured at 490nm for each well, 5 sets of experiments were performed for each set, averaged, and proliferation rate calculated. Proliferation rate (%) = (absorbance value of sample to be measured-absorbance value of control group)/(absorbance value of control group×100%, the result is shown in table 7 below.
TABLE 7
| Test sample | Proliferation promoting rate |
| Example 1 | 128.6％ |
| Example 2 | 130.9％ |
| Example 3 | 127.5％ |
| Comparative example 1 | 128.7％ |
| Comparative example 2 | 93.2％ |
| Comparative example 3 | 99.6％ |
| Comparative example 4 | 110.7％ |
| Comparative example 5 | 84.2％ |
| Comparative example 6 | 87.3％ |
| Comparative example 7 | 126.9％ |
| Comparative example 8 | 125.4％ |
As apparent from the results in Table 7, the glyceroglycolipid of the present invention has an obvious synergistic effect with sodium hyaluronate and acetyl tetrapeptide-5, and the fullerene component in the effect of product repair and anti-aging.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (10)
1. A compound with multiple-effect repairing and anti-aging effects is characterized by comprising freeze-dried powder and solvent liquid matched with the freeze-dried powder; the freeze-dried powder comprises astaxanthin, vitamin E, mannitol, sodium hyaluronate, acetyl tetrapeptide-5, fullerene, polyquaternary ammonium salt-10 and glyceroglycolipid; the solvent liquid comprises water, glycerol, butanediol, centella asiatica extract, passion fruit extract, tremella polysaccharide, water-soluble polysiloxane film forming agent and disodium hydrogen phosphate.
2. The compound with multiple-effect repairing and anti-aging effects according to claim 1, wherein the water-soluble polysiloxane film forming agent is an anionic structure water-soluble polysiloxane, and is prepared by the following method:
adding hydrogen-containing silicone oil and allyl polyoxyethylene ether into an alcohol solvent to be uniformly dissolved, heating to 70-85 ℃, adding a chloroplatinic acid catalyst, stirring and reacting until the alkenyl reaction is complete, adding maleic anhydride, continuously carrying out heat preservation and reacting for 0.5-2 h, and vacuum drying to remove the solvent, thereby obtaining the water-soluble polysiloxane with the anionic structure.
3. The compound with multiple-effect repairing and anti-aging effects according to claim 2, wherein the hydrogen-containing silicone oil is hydrogen-containing silicone oil with hydrogen content of 0.1-0.9% and viscosity of 50-500 mpa.s at 25 ℃; the molecular weight of the allyl polyoxyethylene ether is 200-2000.
4. The compound with multiple-effect repairing and anti-aging effects according to claim 3, wherein the addition amount of the allyl polyoxyethylene ether is 0.05-0.5 times of the hydrogen-containing molar amount of the hydrogen-containing silicone oil.
5. The compound with multiple-effect repairing and anti-aging effects according to claim 4, wherein the addition amount of the maleic anhydride is 0.8-1 times of the molar amount of the allyl polyoxyethylene ether.
6. The compound with multiple-effect repairing and anti-aging effects according to claim 1, wherein the freeze-dried powder comprises the following raw material components in parts by weight:
7. the compound with multiple-effect repairing and anti-aging effects according to claim 1, wherein the solvent liquid comprises the following raw material components in parts by weight:
8. the compound with multiple-effect repairing and anti-aging effects according to claim 1, wherein the ratio of the freeze-dried powder to the solvent is 50-200 mg:1-10 mL.
9. A method for preparing a compound having multiple-effect repairing and anti-aging effects as claimed in any one of claims 1 to 8, comprising the following steps:
(1) Preparing freeze-dried powder: adding astaxanthin, vitamin E and glyceroglycolipid into ethanol, dissolving uniformly, adding fullerene, stirring and dispersing uniformly to obtain ethanol mixed solution; mannitol, sodium hyaluronate, acetyl tetrapeptide-5 and polyquaternium-10 are added into water to be dissolved uniformly, so as to obtain an aqueous solution; homogenizing and mixing the ethanol mixed solution and the aqueous solution, filtering to remove insoluble substances, and vacuum freeze-drying to obtain freeze-dried powder;
(2) Preparing solvent liquid: adding glycerol, butanediol and water-soluble polysiloxane film forming agent into water, stirring and dissolving uniformly, adding herba Centellae extract, herba Passiflorae Caeruleae fruit extract and tremella polysaccharide, stirring and dissolving uniformly, and finally adding disodium hydrogen phosphate and dissolving uniformly to obtain a vehicle solution;
(3) The freeze-dried powder and the solvent liquid are mixed according to a proportion, dissolved and dispersed uniformly to obtain the compound with multiple-effect repairing and anti-aging effects.
10. The method for preparing the compound with multiple-effect repairing and anti-aging effects according to claim 9, wherein in the preparation process of the freeze-dried powder, the mass ratio of ethanol to water is kept within a range of 1-3:1 when the ethanol mixed solution and the aqueous solution are uniformly stirred and mixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311158649.1A CN117017833B (en) | 2023-09-08 | 2023-09-08 | Compound with multiple-effect repairing and anti-aging effects and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311158649.1A CN117017833B (en) | 2023-09-08 | 2023-09-08 | Compound with multiple-effect repairing and anti-aging effects and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117017833A true CN117017833A (en) | 2023-11-10 |
| CN117017833B CN117017833B (en) | 2024-01-23 |
Family
ID=88602513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311158649.1A Active CN117017833B (en) | 2023-09-08 | 2023-09-08 | Compound with multiple-effect repairing and anti-aging effects and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117017833B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003342121A (en) * | 2002-05-28 | 2003-12-03 | Noevir Co Ltd | Skin care preparation |
| CN104177620A (en) * | 2014-07-26 | 2014-12-03 | 张家港格瑞特化学有限公司 | Polyfunctional-group-modified polysiloxane emulsifier and preparation method thereof |
| US20200405610A1 (en) * | 2016-12-29 | 2020-12-31 | Shaanxi Huikang Bio-Tech Co., Ltd. | Cosmetic composition |
| CN115212133A (en) * | 2022-07-25 | 2022-10-21 | 广州丽彦妆生物科技有限公司 | Freeze-dried powder solvent and preparation method and application thereof |
| CN116617145A (en) * | 2023-04-10 | 2023-08-22 | 上海优萃生物科技有限公司 | Rapid anti-aging repair composition and application thereof |
| CN116898766A (en) * | 2023-06-26 | 2023-10-20 | 上海捷丽生物科技有限公司 | A lyophilized liquid, lyophilized film and repairing facial mask containing liposome active substances |
-
2023
- 2023-09-08 CN CN202311158649.1A patent/CN117017833B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003342121A (en) * | 2002-05-28 | 2003-12-03 | Noevir Co Ltd | Skin care preparation |
| CN104177620A (en) * | 2014-07-26 | 2014-12-03 | 张家港格瑞特化学有限公司 | Polyfunctional-group-modified polysiloxane emulsifier and preparation method thereof |
| US20200405610A1 (en) * | 2016-12-29 | 2020-12-31 | Shaanxi Huikang Bio-Tech Co., Ltd. | Cosmetic composition |
| CN115212133A (en) * | 2022-07-25 | 2022-10-21 | 广州丽彦妆生物科技有限公司 | Freeze-dried powder solvent and preparation method and application thereof |
| CN116617145A (en) * | 2023-04-10 | 2023-08-22 | 上海优萃生物科技有限公司 | Rapid anti-aging repair composition and application thereof |
| CN116898766A (en) * | 2023-06-26 | 2023-10-20 | 上海捷丽生物科技有限公司 | A lyophilized liquid, lyophilized film and repairing facial mask containing liposome active substances |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117017833B (en) | 2024-01-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106109296A (en) | A kind of moisturizing essence lotion and preparation method thereof | |
| CN106309154B (en) | One kind having long-acting moisturizing and white-skinned face function skin matrix and its preparation and application | |
| KR100799991B1 (en) | Cosmetic composition for wrinkle care | |
| CN110638722A (en) | Anti-aging cosmetic and preparation method thereof | |
| CN113384506B (en) | Low-irritation plant enzyme whitening nano composition and preparation method and application thereof | |
| CN104887542B (en) | A cosmetic composition | |
| CN113171318A (en) | Freeze-dried powder preparation with moisturizing, whitening, relieving and repairing functions and preparation method thereof | |
| CN111000770A (en) | Whitening cream containing pearl peptide | |
| CN110496067A (en) | A kind of anti-wrinkle composition and its preparation method and application | |
| CN115463039A (en) | Polypeptide repairing anti-allergy freeze-dried powder and preparation method thereof | |
| CN108852969B (en) | Anti-wrinkle anti-aging whitening mask and preparation method thereof | |
| CN111991319B (en) | Repair composition, preparation method and application thereof | |
| CN111671695B (en) | Skin repairing emulsion and application thereof in skin repairing and wrinkle resisting | |
| CN111568781A (en) | Skin care gel and preparation method thereof | |
| CN117017833B (en) | Compound with multiple-effect repairing and anti-aging effects and preparation method thereof | |
| CN112675071A (en) | Wool keratin freeze-dried mask and preparation method and application thereof | |
| CN104546621A (en) | Anti-aging skincare composition and preparation method thereof | |
| CN113893197B (en) | Skin whitening and anti-saccharification soluble beautifying microneedle patch composition and preparation method thereof | |
| CN109106634A (en) | A kind of composition containing goldleaf and red ginseng extract, sleep mask and preparation method thereof | |
| CN113730289B (en) | Eye and lip care composition with multiple repairing effects and application thereof | |
| CN109125189A (en) | A kind of inclusion enclave Essence and preparation method thereof of the instant bright color of energy | |
| CN115813796A (en) | Composite polypeptide freeze-dried eye mask and preparation method thereof | |
| CN117224424B (en) | Whitening, tightening and repairing freeze-dried powder and preparation method and application thereof | |
| CN113730329A (en) | Autologous mucosal cell regeneration and repair composition, preparation method and application thereof | |
| CN113143788A (en) | Multi-effect composition and face cream with moisturizing, repairing and relieving functions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |