CN116982672B - Application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed - Google Patents

Application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed Download PDF

Info

Publication number
CN116982672B
CN116982672B CN202310974849.8A CN202310974849A CN116982672B CN 116982672 B CN116982672 B CN 116982672B CN 202310974849 A CN202310974849 A CN 202310974849A CN 116982672 B CN116982672 B CN 116982672B
Authority
CN
China
Prior art keywords
parts
sugar
feed
betaine
vitamin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202310974849.8A
Other languages
Chinese (zh)
Other versions
CN116982672A (en
Inventor
李红燕
曾妍芝
郁二蒙
谢骏
王广军
龚望宝
田晶晶
张凯
夏耘
李志斐
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pearl River Fisheries Research Institute CAFS
Original Assignee
Pearl River Fisheries Research Institute CAFS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pearl River Fisheries Research Institute CAFS filed Critical Pearl River Fisheries Research Institute CAFS
Priority to CN202310974849.8A priority Critical patent/CN116982672B/en
Publication of CN116982672A publication Critical patent/CN116982672A/en
Application granted granted Critical
Publication of CN116982672B publication Critical patent/CN116982672B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Inorganic Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The invention discloses application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed, and belongs to the technical field of aquaculture. According to the invention, betaine is added into the high-sugar feed to discover that the betaine regulates the expression of genes related to liver sugar metabolism through an AMPK/mTOR (open-pore kinase/mammalian target) channel, so that the ingestion of fish and the utilization efficiency of feed sugar are improved. The invention also provides a feed for improving ingestion and sugar utilization of the fish high-sugar feed, which comprises the following components: fish meal, corn starch, fish oil, multivitamin, polymineral, sodium carboxymethyl cellulose and betaine. The invention proves that the betaine can promote ingestion and sugar utilization of the siniperca chuatsi to the high-sugar feed, and provides a high-sugar feed formula containing the betaine, thereby providing a theoretical basis for improving ingestion and sugar utilization of the siniperca chuatsi high-sugar feed.

Description

Application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed
Technical Field
The invention relates to the technical field of aquaculture, in particular to application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed.
Background
Carbohydrates (sugar) are important nutrient sources for animals, and can be decomposed to generate energy to supply energy for organism life activities, and metabolic intermediate products of the carbohydrates (sugar) can be converted into other carbon-containing compounds, so that amino acids, fat and other nutrient substances are synthesized. The carbohydrate source is abundant and low in price, is widely applied to aquatic feeds, and has the advantages of saving the protein content and the cost of the feeds, protecting fishery resources, reducing the pollution of nitrogen excretion to water environment in the cultivation process, and the like. However, fish have poor feed carbohydrate availability and different feed habits have different tolerance to feed sugar. Compared with omnivorous and herbivorous fishes, the carnivorous fishes are highly intolerant to feed sugar, and are characterized in that continuous postprandial hyperglycemia occurs in the carnivorous fishes such as mandarin fish (SINIPERCA CHUATSI), micropterus salmoides (Micropterus salmoides) and rainbow trout (Oncorhynchus mykiss) after eating high-sugar feed. In addition, the high-sugar feed culture inhibits the growth of aquatic animals, and causes negative effects such as disorder of sugar metabolism of the liver of organisms, fat accumulation and the like.
The phenomenon that the high-sugar feed inhibits fish ingestion is reported in various fishes, and the phenomenon is particularly obvious in carnivorous fish mandarin fish. Mandarin (Siniperca sp.) belongs to Perciformes (Perciformes), and is a unique native fresh water rare fish in China. The mandarin fish has unique feeding habit, and fresh bait is taken as food from the opening, and dead bait and artificial feed are difficult to ingest. The popularization of artificial feed cultivation of mandarin fish is beneficial to reducing environmental pollution caused by bait fish cultivation, reducing cultivation cost and improving quality safety, and is an important direction of the current mandarin fish cultivation industry development. However, as typical fierce carnivorous fishes, mandarin fish is highly intolerant to feed sugar, and the ingestion decline and even anorexia caused by high sugar feed are particularly remarkable, which seriously hinders the growth of mandarin fish and the popularization of large-scale cultivation. The artificial compound feed cultivation of mandarin is the key direction of the current mandarin cultivation industry development, so the problems of anorexia response and sugar utilization induced by high-sugar feed are the bottleneck of the mandarin industry development which needs to be solved urgently.
Disclosure of Invention
The invention aims to provide the application of betaine in improving the ingestion and the sugar utilization of siniperca chuatsi high-sugar feed, so as to solve the problems in the prior art, and the ingestion and the sugar utilization of siniperca chuatsi under the high-sugar condition can be improved due to the addition of the betaine in the high-sugar feed.
In order to achieve the above object, the present invention provides the following solutions:
the invention provides application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed.
The invention also provides application of betaine in preparing feed for improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed.
The invention also provides a high-sugar feed containing betaine, which comprises the following components in parts by weight: 500-1000 parts of fish meal, 150-200 parts of corn starch, 20-30 parts of fish oil, 15-25 parts of vitamin premix, 15-25 parts of mineral premix, 25-35 parts of sodium carboxymethylcellulose and 5-10 parts of betaine.
Preferably, the high-sugar feed comprises the following components in parts by weight: 700 parts of fish meal, 190 parts of corn starch, 30 parts of fish oil, 20 parts of vitamin premix, 20 parts of mineral premix, 30 parts of sodium carboxymethyl cellulose and 10 parts of betaine.
Preferably, the vitamin premix comprises the following components in parts by weight: 10 parts of vitamin A, 16 parts of vitamin B, 25 parts of vitamin B, 7.5 parts of vitamin B, 12 parts of vitamin B, 50 parts of nicotinamide, 500 parts of calcium ascorbyl phosphate, 20 parts of calcium pantothenate, 2.5 parts of biotin, 5 parts of folic acid, 200 parts of vitamin E, 10 parts of vitamin K3, 50 parts of vitamin D, 100 parts of inositol and 75 parts of corn gluten meal.
Preferably, the mineral premix comprises the following components in parts by weight: 10 parts of cupric sulfate pentahydrate, 300 parts of ferrous sulfate monohydrate, 200 parts of zinc sulfate monohydrate, 100 parts of manganese sulfate monohydrate, 80 parts of potassium iodate, 67 parts of sodium selenite, 5 parts of cobalt chloride hexahydrate, 100 parts of sodium chloride and 638 parts of zeolite.
The invention discloses the following technical effects:
in the application of the betaine provided by the invention in improving the ingestion and sugar utilization of siniperca chuatsi high-sugar feed, the betaine regulates the expression of genes related to hypothalamic appetite neuropeptides and liver sugar metabolism through mediating an AMPK/mTOR signal path, so that the ingestion and the utilization efficiency of the siniperca chuatsi high-sugar feed to feed sugar are improved. The action mechanism is as follows: the high-sugar feed obviously reduces the expression of the siniperca chuatsi hypothalamus AMPK and the appetite-promoting gene npy, improves the expression of the appetite-suppressing gene pomc alpha, and obviously promotes the expression of key genes in liver glycolysis and gluconeogenesis processes; the addition of betaine to the high-sugar feed significantly increases hypothalamic anorexic gene npy while suppressing the expression of appetite suppressing gene carb, promotes appetite and significantly increases mandarin Feeding (FI); in addition, betaine is added into the high-sugar feed to induce the expression of liver gluconeogenesis process gene g6pca1 and glycogen synthesis gene gp to be obviously reduced, so that the sugar utilization capacity of siniperca chuatsi on the high-sugar feed is improved.
2. The feed for improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed provided by the invention mediates an AMPK/mTOR signal path through the betaine serving as a feed additive, and promotes ingestion and sugar utilization of siniperca chuatsi.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a graph of growth performance and feed utilization results for NC group, BET group, HC group, and HC+BET group provided by the present invention; SGR: a specific growth rate; FI: food intake; FE: feed efficiency; FR: feeding rate; * P <0.05 represents significant differences, and data are expressed as mean ± standard error;
FIG. 2 shows the basic components of the Siniperca chuatsi body of NC group, BET group, HC group and HC+BET group provided by the invention; * P <0.05 represents significant differences, and data are expressed as mean ± standard error;
Fig. 3 shows that the relative expression levels of the hypothalamus regulatory factors AMPK/mTOR in NC, BET, HC and hc+bet groups, siniperca chuatsi, are significantly different as shown by P <0.05, and the data are shown as mean ± standard error (n=6); AMPK: adenylate activating protein kinase; mTOR: a mammalian rapamycin target;
FIG. 4 shows the relative expression levels of the NC, BET, HC, and HC+BET group siniperca chuatsi hypothalamus appetite genes provided by the present invention; npy: neuropeptide Y; agrp2: neuropeptide squirrel-related proteins; pomc α: opiomelanocortin; the following steps: cocaine and amphetamine regulate transcription peptides;
FIG. 5 shows the relative expression levels of the liver regulatory factors AMPK/mTOR of the NC group, the BET group, the HC group and the HC+BET group siniperca chuatsi;
FIG. 6 shows the relative expression levels of the genes related to glucose metabolism in the liver of Siniperca chuatsi in NC group, BET group, HC group and HC+BET group provided by the invention; gk: a glucokinase; pfk: 6-phosphofructokinase; pk: a pyruvate kinase; pepck: phosphoenolpyruvate carboxykinase; fbp1b:1, 6-bisphosphate fructose; g6pca1: glucose-6-phosphatase; gp: glycogen phosphorylase; gsk: glycogen synthase.
Detailed Description
Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples of the present invention are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.
Example 1
1. Experimental method
1.1 Preparation of feed
Control group feed: the specific formula and chemical composition of the basic fish feed are shown in Table 1.
Betaine group feed: betaine is added into the basic fish feed (the adding ratio of the betaine to the basic fish feed is 10g:1 kg).
High sugar group feed: corn starch is added into the basic fish feed (the adding ratio of the corn starch to the basic fish feed is 200g:1 kg).
High sugar + betaine group feed: corn starch and betaine are added into the basic fish feed (the adding ratio of corn starch, betaine and basic fish feed is 190g:10g:1 kg).
The feed ingredients were thoroughly mixed and granulated, and the pellets were dried in an oven at 50 ℃ and stored at 4 ℃.
Table 1 shows the formulation and chemical composition of each group of feeds
Multidimensional premix mainly containing multiple vitamins; polymineral is a premix containing mainly multiple minerals.
Of the above feeds, 20g of multidimensional/kg feed: vitamin a,10g; vitamin B1,6g; vitamin B2,5g; vitamin B6,7.5g; vitamin B12 (1%), 4g; nicotinamide C 6H6N2 O,50g; calcium ascorbyl phosphate (C 18H24Ca3O26P2, 35%), 500g; calcium pantothenate CHCaN 2 O,20g; biotin (C 10H16N2O3 S, 2%), 2.5g; folic acid, 5g; vitamin E (50%), 200g; vitamin K3, 10g; vitamin D3, 50g; inositol (C 6H12O6), 100g; corn gluten meal, 75g.
Mineral premix, 20g polymineral/kg feed: copper sulfate pentahydrate (CuSO 4·5H2 O), 10g; ferrous sulfate monohydrate (FeSO 4·H2 O), 300g; zinc sulfate monohydrate (ZnSO 4·H2 O), 200g; manganese sulfate monohydrate (MnSO 4·H2 O), 100g; potassium iodate (KIO 3, 10%), 80g; sodium selenite (Na 2SeO3, 10% se), 67g; cobalt chloride hexahydrate (CoCl 2·6H2 O,10% co), 5g; sodium chloride (NaCl), 100g; zeolite 638g.
In the feed components, the percentage in brackets is the mass content.
1.2 Laboratory animals and feeding trials
In the embodiment of the invention, siniperca chuatsi is selected as experimental fish.
All fish were acclimatized to the control diet in the indoor recirculating aquaculture system for two weeks prior to the experiment. Healthy fish (initial body weight: 23.73±0.05 g) of the same specification were then randomly distributed into indoor circulating aquaculture drums (25 tail per drum, r=0.4m, h=1m) and divided into a control group, a betaine group, a high sugar group and a high sugar+betaine group, and three parallel treatments were set for each group.
Daily at 8:30 and 16:00 are fed with feed of a control group, a betaine group, a high sugar group and a high sugar and betaine group respectively, and the culture experiment lasts for 8 weeks. The water temperature is kept at 27.0-28.5 ℃; the pH value is 7.0-8.5; the dissolved oxygen is 4.6-5.5 mg.L -1, and the total ammonia nitrogen is less than 0.1 mg.L -1.
1.3 Sample collection
At the end of the experiment, blood was collected from the tail vein of each barrel of three tail fish using a syringe infiltrated with 0.2% heparin sodium solution. Plasma was obtained by centrifugation at 3000g for 10min at 4 ℃ and was rapidly transferred to-80 ℃ for storage for further analysis. Under ice bath conditions, liver and hypothalamic tissues were rapidly taken, snap frozen in liquid nitrogen and then transferred to-80 ℃ for storage for subsequent enzyme activity and gene expression analysis.
1.4 Biochemical analysis
The chemical composition of the feed and fish samples (crude protein, crude fat, moisture and ash) was determined by the AOAC method. The crude protein content was determined by kjeldahl nitrogen analysis. Crude fat content was determined using a soxhlet extractor.
1.5 Real time quantitative polymerase chain reaction (qRT-PCR)
Total RNA was extracted from the liver and hypothalamus. Using β -actin and rpl13α as housekeeping genes, qRT-PCR was used to detect relevant indicators, including key genes involved in AMPK/mTOR, appetite and sugar metabolism.
1.6 Statistical analysis
The NC, BET, HC and hc+bet groups were compared by single factor analysis of variance using SPSS19.0 software, with P <0.05 (x) being significant. All results are expressed as mean ± standard error.
2. Results and analysis
2.1 Growth Properties, feed utilization and body composition analysis
FIG. 1 shows the effect of betaine addition to high-sugar feeds on siniperca chuatsi growth and feed utilization. Research results show that the application of the feed betaine significantly improves the Specific Growth Rate (SGR) and the ingestion amount (FI) of the mandarin high-sugar feed.
FIG. 2 shows the effect of betaine addition in high sugar feeds on the biochemical composition of siniperca chuatsi bodies. Research results show that the high sugar content of the feed obviously increases the water content of mandarin fish bodies and reduces the ash content of the mandarin fish bodies. The betaine application in the high-sugar feed has no significant influence on the protein, fat, moisture and ash content of mandarin fish bodies.
2.2 Hypothalamic Gene expression
(1) Regulatory factor
The expression level of betaine added into the high-sugar feed on siniperca chuatsi hypothalamus AMPK/mTOR is shown in figure 3. The results of the study showed that the gene expression level of mandarin hypothalamic AMPK was significantly lower in HC group than in NC group, whereas the gene expression level of mTOR was not significantly different from group to group. The addition of feed betaine has no significant effect on hypothalamic AMPK and mTOR expression.
(2) Appetite gene
The expression level of betaine added into the high-sugar feed for the mandarin fish hypothalamus appetite gene is shown in figure 4. The research result shows that compared with NC group, the expression level of the hypothalamus appetite-promoting gene npy in HC group is obviously down-regulated, and the expression level of the appetite-suppressing gene pomc alpha is obviously up-regulated; compared with the HC group, the expression level of the appetite suppressing base agrp2 of the hypothalamus of the HC+BET group is obviously reduced.
2.3 Liver Gene expression
(1) Regulatory factor
The expression level of betaine added into the high-sugar feed on siniperca chuatsi liver AMPK/mTOR is shown in figure 5. Research results show that compared with NC groups, the gene expression quantity of the liver AMPK and mTOR of HC groups is obviously improved; compared with HC, HC+BET group Mandarin liver mTOR gene expression level is remarkably reduced.
(2) Sugar metabolism gene
The expression level of betaine added into the high-sugar feed for the siniperca chuatsi liver sugar metabolism related genes is shown in figure 6. Research results show that the key genes (pfk and pk) of the glycolysis process, the key genes (pepck, fbp1b and g6pca 1) of the liver of mandarin fish fed with high-sugar feed and glycogen metabolism (gp and gsk) are obviously higher than those of the NC group; compared with HC group, the expression level of HC+BET group Mandarin liver g6pca1 and gp is obviously down-regulated, and the expression level of other genes related to sugar metabolism is not obviously different.
From the experimental results, the high-sugar feed can obviously reduce the expression of siniperca chuatsi hypothalamus AMPK, obviously improve the expression of liver AMPK and mTOR, and the application of betaine in the high-sugar feed obviously reduces the expression of siniperca chuatsi liver mTOR. The high-sugar feed can obviously inhibit the ingestion of siniperca chuatsi, the food inhibition is realized by promoting the expression of hypothalamus appetite-suppressing genes pomc alpha and inhibiting the expression of appetite-suppressing genes npy, and the addition of betaine to the high-sugar feed can obviously improve the expression of hypothalamus appetite-suppressing genes npy and inhibit the expression of appetite-suppressing genes cart, so that appetite is promoted and the ingestion (FI) of siniperca chuatsi is obviously improved. In addition, the high-sugar feed obviously improves the expression level of the siniperca chuatsi liver sugar metabolism related genes, and the betaine is added into the high-sugar feed to obviously reduce the expression of the siniperca chuatsi liver g6pca1 and gp, so that the sugar utilization of siniperca chuatsi is improved.
In the present invention, the amount of betaine may be adjusted according to the actual situation, and is not limited to the ratio of betaine in the above examples, but can be used to enhance the feeding and sugar utilization ability of fish by mediating AMPK/mTOR.
It will be appreciated by those skilled in the art that betaine may also be employed in the present invention for enhancing other species of freshwater carnivorous fish, for example: ingestion and sugar utilization of carnivorous fishes such as largemouth black bass and snakehead under high-sugar feed.
In view of the above, the invention provides application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed. The high-sugar feed inhibits the siniperca chuatsi appetite-promoting factor npy and inhibits the appetite-inhibiting factor pomc alpha, and the application of the feed betaine obviously improves the hypothalamus appetite-promoting gene npy under the high-sugar condition of siniperca chuatsi, inhibits the expression of the appetite-inhibiting gene cart and obviously improves the siniperca chuatsi ingestion (FI). Betaine inhibits the expression of liver gluconeogenesis process gene g6pca1 and glycogen synthesis gene gp through mediating AMPK/mTOR signal path, thereby improving the utilization efficiency of siniperca chuatsi on feed sugar. The invention also provides a feed for improving the ingestion and the sugar utilization of the siniperca chuatsi high-sugar feed and a preparation method thereof, wherein the feed comprises basic fish feed, corn starch and betaine for improving the ingestion and the sugar utilization of the siniperca chuatsi; the feed additive betaine improves ingestion and sugar utilization of siniperca chuatsi high-sugar feed by mediating the AMPK/mTOR pathway. The fish is aquatic freshwater carnivorous fish.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.

Claims (5)

1. Use of betaine for improving ingestion and sugar utilization of siniperca chuatsi high sugar feed comprising basal fish feed, corn starch and betaine, each kilogram of the high sugar feed comprising 190g corn starch and 10g betaine;
Betaine is added into the high-sugar feed to improve hypothalamic appetite-promoting gene npy, inhibit the expression of appetite-suppressing gene carb, promote appetite and improve mandarin fish feed intake.
2. Use of betaine for the preparation of a feed for improving ingestion and sugar utilization of a siniperca chuatsi high sugar feed comprising basal fish feed, corn starch and betaine, each kilogram of the high sugar feed comprising 190g corn starch and 10g betaine;
Betaine is added into the high-sugar feed to improve hypothalamic appetite-promoting gene npy, inhibit the expression of appetite-suppressing gene carb, promote appetite and improve mandarin fish feed intake.
3. The use according to claim 1 or 2, characterized in that the components of the high-sugar feed are: 700g of fish meal, 190g of corn starch, 30g of fish oil, 20g of vitamin premix, 20g of mineral premix, 30g of sodium carboxymethyl cellulose and 10g of betaine.
4. The use according to claim 3, wherein the vitamin premix has a composition of: 10 parts of vitamin A, 16 parts of vitamin B, 25 parts of vitamin B, 7.5 parts of vitamin B, 12 parts of vitamin B, 50 parts of nicotinamide, 500 parts of calcium ascorbyl phosphate, 20 parts of calcium pantothenate, 2.5 parts of biotin, 5 parts of folic acid, 200 parts of vitamin E, 10 parts of vitamin K3, 50 parts of vitamin D, 100 parts of inositol and 75 parts of corn gluten meal.
5. The use according to claim 3, wherein the mineral premix has a composition of: 10 parts of cupric sulfate pentahydrate, 300 parts of ferrous sulfate monohydrate, 200 parts of zinc sulfate monohydrate, 100 parts of manganese sulfate monohydrate, 80 parts of potassium iodate, 67 parts of sodium selenite, 5 parts of cobalt chloride hexahydrate, 100 parts of sodium chloride and 638 parts of zeolite.
CN202310974849.8A 2023-08-04 2023-08-04 Application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed Active CN116982672B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310974849.8A CN116982672B (en) 2023-08-04 2023-08-04 Application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310974849.8A CN116982672B (en) 2023-08-04 2023-08-04 Application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed

Publications (2)

Publication Number Publication Date
CN116982672A CN116982672A (en) 2023-11-03
CN116982672B true CN116982672B (en) 2024-05-10

Family

ID=88531751

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310974849.8A Active CN116982672B (en) 2023-08-04 2023-08-04 Application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed

Country Status (1)

Country Link
CN (1) CN116982672B (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007204462A (en) * 2006-01-31 2007-08-16 Sky International:Kk Gluconeogenesis inhibitor in liver and food or food additive containing the same
CN107801865A (en) * 2017-09-18 2018-03-16 佛山市信豚生物科技有限公司 Mandarin fish specific complex premixed feed and preparation method and application
CN108813224A (en) * 2018-07-04 2018-11-16 珠海海龙生物科技有限公司 A kind of adult fish special compound feed and its preparation method and application hybridizing mandarin fish
CN114794329A (en) * 2022-03-29 2022-07-29 唐人神集团股份有限公司 Poulter ilishaeformis fry feed and preparation method thereof
CN115843941A (en) * 2022-11-01 2023-03-28 西北农林科技大学 High-sugar feed for improving sugar utilization and protein deposition rate of carnivorous fishes

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007204462A (en) * 2006-01-31 2007-08-16 Sky International:Kk Gluconeogenesis inhibitor in liver and food or food additive containing the same
CN107801865A (en) * 2017-09-18 2018-03-16 佛山市信豚生物科技有限公司 Mandarin fish specific complex premixed feed and preparation method and application
CN108813224A (en) * 2018-07-04 2018-11-16 珠海海龙生物科技有限公司 A kind of adult fish special compound feed and its preparation method and application hybridizing mandarin fish
CN114794329A (en) * 2022-03-29 2022-07-29 唐人神集团股份有限公司 Poulter ilishaeformis fry feed and preparation method thereof
CN115843941A (en) * 2022-11-01 2023-03-28 西北农林科技大学 High-sugar feed for improving sugar utilization and protein deposition rate of carnivorous fishes

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
不同糖源及糖水平对大菱鲆糖代谢酶活性的影响;聂琴;苗惠君;苗淑彦;陈尘悦;李静;张文兵;麦康森;;水生生物学报;20130515(03);425-433 *
李淑云 ; 刘泓宇 ; 谭北平 ; 董晓慧 ; 杨奇慧 ; 迟淑艳 ; 章双 ; .饲料中糖水平对不同食性海水鱼类PEPCK基因表达和酶活性的影响.水生生物学报.2015,39(01),80-89. *
甜菜碱对单胃动物能量代谢的作用机制与应用;陈星平等;饲料工业;20191231;第40卷(第13期);19-24 *
聂琴 ; 苗惠君 ; 苗淑彦 ; 陈尘悦 ; 李静 ; 张文兵 ; 麦康森 ; .不同糖源及糖水平对大菱鲆糖代谢酶活性的影响.水生生物学报.2013,(03),425-433. *
詹秋羽 ; 王骥腾 ; 韩涛 ; .鱼类摄食调控的研究进展.饲料工业.2020,(16),45-51. *
阎希柱,邱岭泉.饲料中添加甜菜碱对尼罗罗非鱼生长、肌肉组成和消化率的影响.水产学报.1998,(02),186-189. *
饲料中添加甜菜碱对尼罗罗非鱼生长、肌肉组成和消化率的影响;阎希柱, 邱岭泉;水产学报;19980410(02);186-189 *
饲料中糖水平对不同食性海水鱼类PEPCK基因表达和酶活性的影响;李淑云;刘泓宇;谭北平;董晓慧;杨奇慧;迟淑艳;章双;;水生生物学报;20150131;39(01);80-89 *
鱼类摄食调控的研究进展;詹秋羽;王骥腾;韩涛;;饲料工业;20200825(16);45-51 *

Also Published As

Publication number Publication date
CN116982672A (en) 2023-11-03

Similar Documents

Publication Publication Date Title
Shiau et al. Carbohydrate utilization and digestibility by tilapia, Oreochromis niloticus× O. aureus, are affected by chromic oxide inclusion in the diet
Tan et al. Zinc methionine and zinc sulfate as sources of dietary zinc for juvenile abalone, Haliotis discus hannai Ino
Ballestrazzi et al. The effect of dietary protein level and source on growth, body composition, total ammonia and reactive phosphate excretion of growing sea bass (Dicentrarchus labrax)
Cheng et al. Nutritional value of diets containing distiller's dried grain with solubles for rainbow trout, Oncorhynchus mykiss
Saunderson et al. Changes in body-weight, composition and hepatic enzyme activities in response to dietary methionine, betaine and choline levels in growing chicks
Manush et al. Dietary high protein and vitamin C mitigate stress due to chelate claw ablation in Macrobrachium rosenbergii males
Liu et al. Resveratrol inclusion alleviated high-dietary-carbohydrate-induced glycogen deposition and immune response of largemouth bass, Micropterus salmoides
Shan et al. Comparative study of the key enzymes and biochemical substances involved in the energy metabolism of Pacific white shrimp, Litopenaeus vannamei, with different ammonia-N tolerances
Arnesen et al. Crude and pre-extruded products of wheat as nutrient sources in extruded diets for Atlantic salmon (Salmo salar, L) grown in sea water
Lim et al. Effects of dietary supplementation of copper chelates in the form of methionine, chitosan and yeast in laying hens
Wang et al. Molecular response of carbohydrate metabolism to dietary carbohydrate and acute low salinity stress in Pacific white shrimp Litopenaeus vannamei
Xu et al. Dietary calcium requirement of red swamp crayfish (Procambarus clarkia)
Gamboa-Delgado et al. Comparing the assimilation of dietary nitrogen supplied by animal-, plant-and microbial-derived ingredients in Pacific white shrimp Litopenaeus vannamei: A stable isotope study
Satoh et al. Bioavailability of amino acid‐chelated and glass‐embedded manganese to rainbow trout, Oncorhynchus mykiss (Walbaum), fingerlings
Feng et al. Alterations of amino acid metabolism and intestinal microbiota in Chinese mitten crab (Eriocheir sinensis) fed on formulated diet and iced trash fish
Davis et al. Estimation of apparent phosphorus availability from inorganic phosphorus sources for Penaeus vannamei
CN116982672B (en) Application of betaine in improving ingestion and sugar utilization of siniperca chuatsi high-sugar feed
Okorie et al. Preliminary study of the optimum dietary riboflavin level in sea cucumber, Apostichopus japonicus (Selenka)
Zhu et al. The Potential Advantage of Myo‐Inositol as a Dietary Supplement for Juvenile Hybrid Grouper (Brown‐Marbled Grouper Epinephelus fuscoguttatus♀× Giant Grouper Epinephelus lanceolatus♂)
CN114271413A (en) Feed for improving utilization rate of sugar in grass carp and preparation and application of feed
Chen et al. Comparative study on the utilization of crystalline methionine and methionine hydroxy analogue calcium by Pacific white shrimp (Litopenaeus vannamei Boone)
Wang et al. Chromium polynicotinate inclusion in the diet affects growth, feed utilization, and chromium retention in Japanese seabass Lateolabrax japonicas fed a low protein starch-based diet
Syrovatko The efficiency of using a feed acidifier in growing broiler chickens
CN104642806A (en) Feed capable of improving anti-stress capability of large yellow croakers
CN111685243A (en) Compound premix feed and application thereof in aquaculture

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant