CN116973424A - Microorganism electrochemical sensor testing system and method - Google Patents
Microorganism electrochemical sensor testing system and method Download PDFInfo
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- CN116973424A CN116973424A CN202311230134.8A CN202311230134A CN116973424A CN 116973424 A CN116973424 A CN 116973424A CN 202311230134 A CN202311230134 A CN 202311230134A CN 116973424 A CN116973424 A CN 116973424A
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- 238000012360 testing method Methods 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 29
- 244000005700 microbiome Species 0.000 title description 4
- 239000007788 liquid Substances 0.000 claims abstract description 549
- 230000000813 microbial effect Effects 0.000 claims abstract description 103
- 238000002347 injection Methods 0.000 claims abstract description 30
- 239000007924 injection Substances 0.000 claims abstract description 30
- 238000007599 discharging Methods 0.000 claims abstract description 19
- 238000007789 sealing Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 101
- 239000003153 chemical reaction reagent Substances 0.000 claims description 94
- 239000002699 waste material Substances 0.000 claims description 38
- 238000003756 stirring Methods 0.000 claims description 11
- 238000001514 detection method Methods 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 6
- 230000001988 toxicity Effects 0.000 claims description 6
- 231100000419 toxicity Toxicity 0.000 claims description 6
- 230000009471 action Effects 0.000 claims description 5
- 230000005484 gravity Effects 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 4
- 239000003792 electrolyte Substances 0.000 claims description 4
- 238000010998 test method Methods 0.000 claims description 4
- 239000011550 stock solution Substances 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 description 10
- 230000007774 longterm Effects 0.000 description 7
- 238000007664 blowing Methods 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000002156 mixing Methods 0.000 description 5
- 239000011259 mixed solution Substances 0.000 description 4
- 238000005086 pumping Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N27/416—Systems
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
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Abstract
The invention discloses a microbial electrochemical sensor testing system and a method. The system comprises a feeding device, a power pump, a protection device, a first three-way valve, a microbial electrochemical sensor and a second three-way valve, wherein the protection device comprises a liquid storage cavity which can be arranged in a sealing manner, the liquid storage cavity is sequentially provided with an exhaust port, a second liquid level sensor and a first liquid level sensor from top to bottom, the exhaust port is connected with the first stop valve, and the setting heights of the first liquid level sensor and the second liquid level sensor on the liquid storage cavity sequentially correspond to a preset exhaust liquid level line and a preset maximum liquid injection line; the inlet of the liquid storage cavity is connected with the output end of the power pump through a pipeline, the pipeline stretches into the liquid storage cavity, and the outlet of the pipeline is lower than the first liquid level sensor; and a bubble sensor is arranged between the second stop valve and the liquid outlet of the microbial electrochemical sensor. The system can effectively remove bubbles at the front end of the microbial electrochemical sensor, has no limit capacity of expanding and discharging bubbles, and solves the problem that the electric signal is influenced due to the existence of bubbles.
Description
Technical Field
The invention belongs to the field of biochemistry, and particularly relates to a system and a method for testing a microbial electrochemical sensor.
Background
The microbial electrochemical sensor (MEB) taking electrochemical active microorganisms (EAB) as a core can directly convert substances to be detected in a water body into bioelectric signals, has the advantages of rapid detection, high sensitivity, low detection cost, strong anti-interference capability and the like, and has good application prospects in the fields of biomedicine and environmental monitoring. However, the conditions are high when the microbial electrochemical sensor is used online, especially when carbon felt, carbon cloth and the like are used as microbial adsorption materials, bubbles are easy to block on the surface of the microbial electrochemical sensor so as to have a large influence on an electric signal, further toxicity detection is influenced, the application requirements of long-term online stable monitoring of water quality biotoxicity cannot be met, and when air leakage occurs in the system, a protective solution is lacking in the sensor, so that the microbial electrochemical sensor is damaged.
Disclosure of Invention
The present invention aims to solve at least one of the technical problems in the related art to some extent. To this end, an object of the present invention is to propose a system and a method for testing a microbial electrochemical sensor. The test system is provided with the protection device, so that bubbles at the front end of the microbial electrochemical sensor can be effectively removed, the capacity of the bubbles is not limited, the problem that electric signals are influenced due to the existence of the bubbles is solved, the stability of the microbial electrochemical sensor is further improved, the service life of the microbial electrochemical sensor is prolonged, and the online long-term stable monitoring of the water quality biotoxicity is realized.
A first aspect of the present invention proposes a microbial electrochemical sensor testing system. According to an embodiment of the invention, the system comprises:
the device comprises an air inlet, a pure water inlet, a water sample inlet to be tested, at least one reagent inlet, a circulating liquid inlet and a plurality of valves;
the input end of the power pump is switchably connected with the air inlet, the pure water inlet, the water sample inlet to be tested, the reagent inlet and the circulating liquid inlet through the plurality of valves;
the protection device comprises a liquid storage cavity which can be arranged in a sealing manner, an exhaust port, a second liquid level sensor and a first liquid level sensor are sequentially arranged in the liquid storage cavity from top to bottom, the exhaust port is connected with a first stop valve, the arrangement height of the first liquid level sensor on the liquid storage cavity corresponds to a preset exhaust liquid level line, and the arrangement height of the second liquid level sensor on the liquid storage cavity corresponds to a preset maximum liquid injection line; the inlet of the liquid storage cavity is connected with the output end of the power pump through a pipeline, the pipeline stretches into the liquid storage cavity, and the outlet of the pipeline is lower than the first liquid level sensor;
The first three-way valve comprises a first public end, a first discharge end and a first waste liquid end, and the first public end is connected with an outlet of the liquid storage cavity;
the liquid inlet of the microbial electrochemical sensor is connected with the first discharge end;
the second three-way valve comprises a second public end, a second discharge end and a second waste liquid end, the second public end is connected with a liquid outlet of the microbial electrochemical sensor through a second stop valve, a bubble sensor is arranged between the second stop valve and the liquid outlet of the microbial electrochemical sensor, and the second discharge end is connected with the circulating liquid inlet.
The microbial electrochemical sensor testing system of the embodiment of the invention has at least the following advantages: 1. the method can be combined with a protection device to effectively remove bubbles at the front end of the microbial electrochemical sensor before and during the test, has no capacity expansion and bubble discharge capacity, solves the problem that the electric signal is influenced by bubbles, further improves the stability of the microbial electrochemical sensor, prolongs the service life of the microbial electrochemical sensor and realizes the online long-term stable monitoring of the water biotoxicity; 2. the bubble sensor can be used for identifying whether the bubble quantity in a pipeline between the microbial electrochemical sensor and the second stop valve exceeds a preset critical value, judging whether the microbial electrochemical sensor is likely to be disconnected or not based on the identified signal, and stopping the operation if the power pump and the second stop valve are required to be closed so as to prevent the microbial electrochemical sensor from leaking liquid and protect the microbial electrochemical sensor; 3. the liquid level sensor can be used for judging the liquid level position in the liquid storage cavity and judging whether liquid is required to be replenished into the liquid storage cavity or whether the exhaust operation is required to be carried out by combining the protection device or not based on the identification result; 4. the protection device is convenient to clean and change liquid.
In addition, the microbial electrochemical sensor testing system according to the above embodiment of the present invention may further have the following additional technical features:
in some embodiments of the invention, the at least one reagent inlet comprises a first reagent inlet and a second reagent inlet, the first reagent is suitable for being used as electrolyte in the microbial electrochemical sensor, and the second reagent is suitable for being mixed with a water sample to be tested for water toxicity detection.
In some embodiments of the invention, the first liquid level sensor and the second liquid level sensor are provided on an outer wall of the liquid storage chamber.
In some embodiments of the invention, the bubble sensor is an ultrasonic sensor.
In some embodiments of the present invention, at least one third liquid level sensor is disposed on an outer wall of the liquid storage cavity, the third liquid level sensor is disposed lower than the second liquid level sensor, and a height of the third liquid level sensor on the outer wall of the liquid storage cavity is adjustable.
In some embodiments of the present invention, a plurality of third liquid level sensors are disposed on an outer wall of the liquid storage cavity at intervals, and the setting heights of the plurality of third liquid level sensors on the liquid storage cavity correspond to different preset liquid injection lines.
In some embodiments of the invention, the protection device comprises: at least two spheres arranged up and down, the exhaust port being provided at the upper part of the sphere at the top; the two adjacent spheres are communicated through the first cylindrical pipe; the second column tube, the second column tube with be located the bottom the spheroid intercommunication sets up and is located this spheroid below, first level sensor is higher than the second column tube sets up, the second column tube with first column tube with spheroidal inner chamber combination forms the stock solution chamber.
In some embodiments of the invention, the microbial electrochemical sensor testing system further comprises: the automatic control unit is connected with the multiple valves, the power pump, the first stop valve, the first liquid level sensor, the second liquid level sensor, the first three-way valve, the bubble sensor, the second stop valve and the second three-way valve, is suitable for receiving liquid level signals identified by the first liquid level sensor and the second liquid level sensor and bubble quantity signals identified by the bubble sensor, and controls based on a preset program: the power pump, the first stop valve and the second stop valve are opened and closed, and the ports of the multiple valves, the first three-way valve and the second three-way valve are switched, so that the automatic operation and the exhaust of the preset program are realized; the preset program comprises a culture test program and a cleaning program.
The second aspect of the invention provides a method for testing water quality biotoxicity by using the microbial electrochemical sensor testing system. According to an embodiment of the invention, the method comprises:
(1) Closing a second stop valve, opening a first stop valve, switching a first three-way valve to a first discharge end, switching an input end of a power pump to a reagent inlet or a water sample inlet to be detected through a plurality of valves, and starting the power pump to pump the reagent into a liquid storage cavity or respectively pump the reagent and the water sample to be detected into the liquid storage cavity; when the second liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset maximum liquid injection line, the first stop valve is closed;
(2) Switching the first three-way valve to a first waste liquid end, and discharging gas in a pipeline between the protection device and the first three-way valve;
(3) Closing the power pump, switching the first three-way valve to the first discharge end, opening the second stop valve, and switching the second three-way valve to the second waste liquid end; starting the power pump to replace the liquid in the microbial electrochemical sensor;
(4) Closing the power pump, switching the input end of the power pump to a circulating liquid inlet through the plurality of valves, and switching the second three-way valve to a second discharge end; starting the power pump, circularly culturing,
Wherein: in the step (4), when the first liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset exhaust liquid level line, the power pump is turned off, and the operations of the steps (1) - (4) are repeated; and when the bubble sensor recognizes that the bubble quantity in the pipeline between the microbial electrochemical sensor and the second stop valve is not lower than a preset critical value, closing the power pump and the second stop valve, and stopping operation.
The method for testing the biotoxicity of water quality according to the embodiment of the invention has at least the following advantages: 1. the method can be combined with a protection device to effectively remove bubbles at the front end of the microbial electrochemical sensor before and during the test, has no capacity expansion and bubble discharge capacity, solves the problem that the electric signal is influenced by bubbles, further improves the stability of the microbial electrochemical sensor, prolongs the service life of the microbial electrochemical sensor and realizes the online long-term stable monitoring of the water biotoxicity; 2. the bubble sensor can be used for identifying whether the bubble quantity in a pipeline between the microbial electrochemical sensor and the second stop valve exceeds a preset critical value, judging whether the microbial electrochemical sensor is likely to be disconnected or not based on the identified signal, and stopping the operation if the power pump and the second stop valve are required to be closed so as to prevent the microbial electrochemical sensor from leaking liquid and protect the microbial electrochemical sensor; 3. the liquid level sensor can be used for judging the liquid level position in the liquid storage cavity and judging whether liquid is required to be replenished into the liquid storage cavity or whether the exhaust operation is required to be carried out by combining the protection device or not based on the identification result; 4. the protection device is convenient to clean and change liquid.
In some embodiments of the invention, before performing step (1) and/or after performing step (4) further comprises: the method for discharging the residual liquid in the liquid storage cavity and cleaning the liquid storage cavity comprises the following specific steps: (a) Closing the first stop valve and the second stop valve, switching the first three-way valve to the first waste liquid end, switching the input end of the power pump to an air inlet through the plurality of valves, and starting the power pump to discharge residual liquid in the liquid storage cavity; (b) Closing the power pump, opening the first stop valve, switching the first three-way valve to the first discharge end, and switching the input end of the power pump to the pure water inlet through the plurality of valves; starting the power pump to clean the liquid storage cavity and the connecting pipeline; when the second liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset maximum liquid injection line, the power pump is turned off within a preset time; (c) And closing the first stop valve, switching the first three-way valve to the first waste liquid end, switching the input end of the power pump to the air inlet through the plurality of valves, and starting the power pump to discharge water in the liquid storage cavity and the connecting pipeline.
In some embodiments of the invention, step (a) comprises: (a-1) opening the first shut-off valve and closing the second shut-off valve, switching the first three-way valve to the first waste liquid end, and discharging residual liquid in the liquid storage cavity under the action of gravity; (a-2) closing the first shut-off valve, switching the input of the power pump to the air inlet through the plurality of valves, and starting the power pump to further drain the residual liquid in the liquid storage chamber.
In some embodiments of the invention, a method of testing water quality for biotoxicity comprises: sequentially performing steps (1) - (4), steps (a) - (c) and steps (5) - (10), wherein: in the step (1), the second stop valve is closed, the first stop valve is opened, the first three-way valve is switched to the first discharge end, the input end of the power pump is switched to a first reagent inlet through the plurality of valves, and the power pump is started to pump a first reagent into the liquid storage cavity; when the second liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset maximum liquid injection line, the first stop valve is closed; the steps (5) - (10) comprise: (5) Closing the second stop valve, opening the first stop valve, switching the first three-way valve to the first discharge end, switching the input end of the power pump to a second reagent inlet through the plurality of valves, and starting the power pump to pump a second reagent into the liquid storage cavity; when the volume of the second reagent in the liquid storage cavity reaches a first preset volume, the power pump is turned off; switching the input end of the power pump to a water sample inlet to be detected through the plurality of valves, and starting the power pump to pump the water sample to be detected into the liquid storage cavity; when the second liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset maximum liquid injection line, the power pump is turned off; (6) Switching the input end of the power pump to the air inlet through the multiple valves, and starting the power pump to blow and stir; (7) Closing the first stop valve, switching the first three-way valve to the first waste liquid end, and discharging gas in a pipeline between the protection device and the first three-way valve; (8) Closing the power pump, opening the first stop valve, switching the first three-way valve to the first discharge end, switching the input end of the power pump to the second reagent inlet through the plurality of valves, and starting the power pump to pump a second reagent with a second preset volume into the liquid storage cavity; closing the power pump; switching the input end of the power pump to the water sample inlet to be detected through the plurality of valves, and starting the power pump to pump the water sample to be detected into the liquid storage cavity; when the second liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset maximum liquid injection line, the power pump is turned off; the volume of the water sample to be detected pumped into the liquid storage cavity in the step (8) is V2, the volume of the water sample to be detected pumped into the liquid storage cavity in the step (5) is V1, and the ratio of the second preset volume to V2 is equal to the ratio of the first preset volume to V1; (9) Switching the input end of the power pump to an air inlet through the multiple valves, and starting the power pump to blow and stir; (10) Closing the power pump, switching the first three-way valve to the first discharge end, opening the second stop valve, and switching the second three-way valve to the second discharge end; starting the power pump, and circularly culturing, wherein: in the step (10), when the first liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset exhaust liquid level line, the power pump is turned off, and the operations of the steps (5) - (10) are repeated; and when the bubble sensor recognizes that the bubble quantity in the pipeline between the microbial electrochemical sensor and the second stop valve is not lower than a preset critical value, closing the power pump and the second stop valve, and stopping operation.
In some embodiments of the present application, two third liquid level sensors are disposed on the outer wall of the liquid storage cavity, wherein one of the third liquid level sensors is lower than the first liquid level sensor, and the disposed height on the liquid storage cavity corresponds to the liquid level line of the first preset volume, and the other third liquid level sensor is higher than the first liquid level sensor, and the height difference between the third liquid level sensor and the first liquid level sensor corresponds to the liquid level height of the second preset volume in the liquid storage cavity.
Drawings
The foregoing and/or additional aspects and advantages of the application will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic structural view of a microbial electrochemical sensor testing system according to one embodiment of the present application.
FIG. 2 is a schematic structural view of a microbial electrochemical sensor testing system according to yet another embodiment of the present application.
Reference numerals:
10-feeding devices; 11-an air inlet; 12-pure water inlet; 13-a water sample inlet to be detected; 14-reagent inlet; 14 a-a first reagent inlet; 14 b-a second reagent inlet; 15-a circulating liquid inlet; 16-multiple valves;
20-a power pump;
30-a protection device; 31-a liquid storage cavity; 31 a-sphere; 31 b-a first cylindrical tube; 31 c-a second cylindrical tube; 32-exhaust port; 33-a first level sensor; 34-a second level sensor; 35-piping; 36-a third level sensor; 36 a-one of the third level sensors; 36 b-another third level sensor;
40 a-a first shut-off valve; 40 b-a second shut-off valve;
50-a first three-way valve; 51-a first common terminal; 52-a first discharge end; 53-a first waste stream end;
60-microbial electrochemical sensor;
70-a second three-way valve; 71-a second common terminal; 72-a second discharging end; 73-a second waste stream end;
80-bubble sensor.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present invention and should not be construed as limiting the invention.
The current microbial electrochemical sensor is generally directly applied on site without adding sensor protection measures, and in actual use, the sensor fault problem is often caused by unstable test data caused by the fact that bubbles are attached to electrodes and a large number of bubbles exist. At present, most of bubble removing devices in water quality monitoring are additionally provided with blocking nets, the structure is complex, bubbles at the front end of a water outlet can be removed, bubbles still can be generated in a pipeline at the rear end, and the blocking nets which are blocked are easy to adhere to microorganisms to cause strain change, so that the performance of a microbial electrochemical sensor is affected, and the requirement of on-line monitoring of the microbial electrochemical sensor cannot be met.
In view of this, a first aspect of the present invention proposes a microbial electrochemical sensor testing system. As understood with reference to fig. 1, according to an embodiment of the present invention, the system includes: the device comprises a feeding device 10, a power pump 20, a protection device 30, a first three-way valve 50, a microbial electrochemical sensor 60 and a second three-way valve 70. Wherein the feeding device 10 comprises an air inlet 11, a pure water inlet 12, a water sample inlet 13 to be tested, at least one reagent inlet 14, a circulating liquid inlet 15 and a plurality of valves 16; the input end of the power pump 20 is switchably connected with the air inlet 11, the pure water inlet 12, the water sample inlet 13 to be tested, the reagent inlet 14 and the circulating liquid inlet 15 through a plurality of valves 16; the protection device 30 comprises a liquid storage cavity 31 which can be arranged in a sealing manner, wherein the liquid storage cavity 31 is sequentially provided with an air outlet 32, a second liquid level sensor 34 and a first liquid level sensor 33 from top to bottom, the air outlet 32 is connected with a first stop valve 40a, the setting height of the first liquid level sensor 33 on the liquid storage cavity 31 corresponds to a preset air exhaust liquid level line, and the setting height of the second liquid level sensor 34 on the liquid storage cavity 31 corresponds to a preset maximum liquid injection line; the inlet of the liquid storage cavity 31 is connected with the output end of the power pump 20 through a pipeline 35, the pipeline 35 stretches into the liquid storage cavity 31, and the outlet of the pipeline 35 is lower than the first liquid level sensor 33; the first three-way valve 50 comprises a first public end 51, a first discharge end 52 and a first waste liquid end 53, and the first public end 51 is connected with the outlet of the liquid storage cavity 31; the liquid inlet of the microbial electrochemical sensor 60 is connected with the first discharging end 52; the second three-way valve 70 comprises a second public end 71, a second discharge end 72 and a second waste liquid end 73, the second public end 71 is connected with a liquid outlet of the microbial electrochemical sensor 60 through a second stop valve 40b, a bubble sensor 80 is arranged between the second stop valve 40b and the liquid outlet of the microbial electrochemical sensor 60, and the second discharge end 72 is connected with the circulating liquid inlet 15.
The microbial electrochemical sensor testing system of the embodiment of the invention has at least the following advantages: 1. the method can be combined with a protection device to effectively remove bubbles at the front end of the microbial electrochemical sensor before and during the test, has no capacity expansion and bubble discharge capacity, solves the problem that the electric signal is influenced by bubbles, further improves the stability of the microbial electrochemical sensor, prolongs the service life of the microbial electrochemical sensor and realizes the online long-term stable monitoring of the water biotoxicity; 2. the bubble sensor is suitable for identifying whether the bubble amount in the pipeline between the microbial electrochemical sensor and the second stop valve exceeds a preset critical value, when the bubble sensor identifies that the bubble amount in the pipeline between the microbial electrochemical sensor and the second stop valve is not lower than a signal of the preset critical value, the bubble sensor indicates that the rear end of the microbial electrochemical sensor possibly has air leakage and is possibly broken, thereby the bubble sensor can be used for identifying whether the bubble amount in the pipeline between the microbial electrochemical sensor and the second stop valve exceeds the preset critical value, and judging whether the microbial electrochemical sensor is possibly broken or not based on the identified signal, and whether the power pump and the second stop valve are required to be closed or not, so that the microbial electrochemical sensor is prevented from leaking liquid and is protected; 3. the liquid level sensor can be used for judging the liquid level position in the liquid storage cavity and judging whether liquid is required to be replenished into the liquid storage cavity or whether the exhaust operation is required to be carried out by combining the protection device or not based on the identification result; 4. the protection device is convenient to clean and change liquid; 5. the pipeline 35 stretches into the liquid storage cavity, and the outlet of the pipeline 35 is lower than the first liquid level sensor, so that liquid can be prevented from being splashed or blown for stirring.
As understood with reference to fig. 1 or 2, specific operations for effectively removing bubbles from the front end of the microbial electrochemical sensor before and during testing in conjunction with the protection device 30 according to an embodiment of the present invention may include:
(i) Closing the second stop valve 40b, opening the first stop valve 40a, switching the first three-way valve 50 to the first discharge end 52, switching the input end of the power pump 20 to the reagent inlet 14 or the water sample inlet 13 to be tested through a plurality of valves 16 (according to actual test requirements), and starting the power pump 20 to pump the reagent into the liquid storage cavity 31 or respectively pump the reagent and the water sample to be tested into the liquid storage cavity 31; when the second liquid level sensor 34 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset maximum liquid injection line, the first stop valve 40a is closed; (ii) Switching the first three-way valve 50 to the first waste end 53 to discharge the gas in the line between the protection device 30 and the first three-way valve 50; (iii) Closing the power pump 20, switching the first three-way valve 50 to the first discharge end 52, opening the second stop valve 40b, and switching the second three-way valve 70 to the second waste end 73; activating the power pump 20 to displace the liquid in the microbial electrochemical sensor 60; (iv) Closing the power pump 20, switching the input end of the power pump 20 to the circulating liquid inlet 15 through the plurality of valves 16, and switching the second three-way valve 70 to the second discharge end 72; the power pump 20 is started, and the culture is circulated.
The operation can effectively remove bubbles at the front end of the microbial electrochemical sensor before testing. For the bubbles generated during the test, the bubbles can be discharged based on the signal identified by the first liquid level sensor 33, specifically, in step (iv), when the first liquid level sensor 33 identifies that the liquid level of the liquid storage cavity 31 reaches the signal of the preset exhaust liquid level line, the power pump 20 is turned off, and the operations of steps (i) - (iv) are repeated. In the testing process, when the front end of the pipeline has bubbles or the reagent is not sucked, the bubbles stay at the upper end position of the liquid level, the liquid level in the liquid storage cavity 31 in the protection device 30 can be reduced, but the testing is not influenced at this time, when the liquid level is reduced to the first liquid level sensor 33, the first liquid level sensor 33 recognizes the signal, and the operation of filling the reagent or the operation of filling the reagent and the water sample to be tested is repeated again, so that the bubbles and the accumulated air can be removed from the air outlet 32, the bubbles are prevented from entering the microbial electrochemical sensor 60, the stability of the microbial electrochemical sensor 60 is maintained, and the stability and the accuracy of the testing result are further ensured. In addition, when the bubble sensor 80 recognizes a signal that the amount of bubbles in the pipe line between the microbial electrochemical sensor 60 and the second shut-off valve 40b is not lower than a preset critical value (recognition of the signal indicates that there is a possibility of gas leakage and disconnection at the rear end of the microbial electrochemical sensor), it is judged that the microbial electrochemical sensor 60 is in a state where protection is required, at this time, the power pump 20 and the second shut-off valve 40b may be closed, the operation may be stopped (to take corresponding measures against the problem), the sensor may be protected and alarm may be given, the microbial electrochemical sensor may be prevented from leaking, and the microbial electrochemical sensor may be protected. Alternatively, the bubble sensor 80 may be connected with an alarm device configured to emit an alarm signal when the bubble sensor 80 recognizes that the amount of bubbles in the pipe between the microbial electrochemical sensor 60 and the second shut-off valve 40b is not lower than a preset critical value, which may be an indicator lamp and/or an alarm sound.
According to an embodiment of the present invention, when switching the reagent, or before introducing the reagent into the protection device 30, the protection device 30 may be further cleaned and replaced, as understood with reference to fig. 1 or fig. 2, including: the steps of draining the residual liquid in the liquid storage chamber 31 and cleaning the liquid storage chamber 31 may include: (I) Closing the first stop valve 40a and the second stop valve 40b, switching the first three-way valve 50 to the first waste liquid end 53, switching the input end of the power pump 20 to the air inlet 11 through the plurality of valves 16, and starting the power pump 20 to discharge the residual liquid in the liquid storage cavity 31; (II) closing the power pump 20, opening the first shut-off valve 40a, switching the first three-way valve 50 to the first discharge end 52, switching the input end of the power pump 20 to the pure water inlet 12 through the plurality of valves 16; starting the power pump 20 to clean the liquid storage cavity 31 and connecting pipelines (including pipelines between the power pump 20 and the plurality of valves 16, between the protection device 30 and the power pump 20 and between the first three-way valve 50 and the liquid storage cavity 31); when the second liquid level sensor 34 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset maximum liquid injection line, the power pump 20 is turned off within the preset time (the preset time can be flexibly selected based on the actual conditions of the liquid storage cavity capacity between the maximum liquid injection line and the exhaust port, the liquid passing flow rate in unit time of the power pump and the like, for example, the preset time can be 0 s-3 s, 2s and the like, so that the cleaning effect can be further ensured); (III) closing the first shut-off valve 40a, switching the first three-way valve 50 to said first waste side 53, switching the input of the power pump 20 to the air inlet 11 via the multiple valves 16; the power pump 20 is started to discharge the water in the liquid storage cavity 31 and the connecting pipeline. Preferably, step (I) may further comprise: (I-1) opening the first shut-off valve 40a and closing the second shut-off valve 40b, switching the first three-way valve 50 to the first waste liquid end 53, and discharging the residual liquid in the liquid storage chamber 31 under the action of gravity; (I-2) closing the first shut-off valve 40a, switching the input of the power pump 20 to the air inlet 11 through the multiple valves 16, starting the power pump 20 to further drain the residual liquid in the liquid storage chamber 31. By adopting the mode, the residual liquid in the liquid storage cavity can be effectively discharged, and the pure water is used for cleaning, so that the accuracy of the detection result is improved.
In some embodiments of the present invention, as will be appreciated with reference to fig. 1 or 2, the at least one reagent inlet may comprise a first reagent inlet 14a adapted to act as an electrolyte in the microbial electrochemical sensor 60 and a second reagent inlet 14b adapted to be mixed with a water sample to be tested for water toxicity detection. The first reagent and the second reagent may have the same composition, and may differ only in the concentration of the active ingredient in the two reagents. The first reagent is suitable for replacing the original electrolyte in the microbial electrochemical sensor 60, the mixed solution obtained by mixing the second reagent with the water sample to be tested can be understood as that the second reagent is diluted by the water sample to be tested, and the concentration of each component in the diluted second reagent in the mixed solution can be the same as that of the first reagent, so that the first reagent and the mixed solution of the second reagent and the water sample to be tested can be only different from each other in the components of the water sample to be tested relative to pure water, and at the moment, the electric signal of the microbial electrochemical sensor 60 for circulating culture in the first reagent can be used as a reference to be compared with the electric signal of the microbial electrochemical sensor 60 for circulating culture in the mixed solution of the second reagent and the water sample to be tested, so that the toxicity detection result of the water sample to be tested is obtained. By arranging the first reagent inlet and the second reagent inlet, the on-line operation is facilitated, and the operation flow is simplified.
In some embodiments of the present invention, it is understood with reference to fig. 1 or 2 that the first liquid level sensor 33 and the second liquid level sensor 34 may be provided on an outer wall of the liquid storage chamber 31. By adopting the arrangement, on one hand, whether the liquid level sensor has liquid at the set height can be judged based on the signal change of the liquid level sensor, and on the other hand, the set heights of the first liquid level sensor 33 and the second liquid level sensor 34 can be flexibly adjusted according to a preset exhaust liquid level line and a preset maximum liquid injection line of the liquid storage cavity 31.
In some embodiments of the present invention, the specific type of the bubble sensor 80 is not particularly limited, and a person skilled in the art may flexibly select according to actual needs, for example, may be an ultrasonic sensor, etc., as long as a signal that can identify whether the amount of bubbles in the tube exceeds a preset critical value is provided. The air bubble sensor 80 is used to more sensitively determine whether the microbial electrochemical sensor 60 is in a state requiring protection, for example, as a specific example, when the air bubble amount identified by the air bubble sensor 80 exceeds a signal of a preset critical value, the microbial electrochemical sensor 60 is determined to be in a state requiring protection, and at this time, the power pump 20 and the second stop valve 40b can be closed to prevent more air bubbles from entering the microbial electrochemical sensor 60, protect the sensor and alarm, and repeat the operations of steps (i) - (iv).
In some embodiments of the present invention, it is understood with reference to fig. 2 that at least one third liquid level sensor 36 may be provided on the outer wall of the liquid storage chamber 31, the third liquid level sensor 36 may be provided lower than the second liquid level sensor 34, and the height of the third liquid level sensor 36 on the outer wall of the liquid storage chamber 31 may be adjustable. By arranging the third liquid level sensor 36, the setting height of the third liquid level sensor 36 can be further adjusted according to actual requirements, such as the ratio of the second reagent to the water sample to be measured.
Further, as understood with reference to fig. 2, a plurality of third liquid level sensors 36 arranged at intervals up and down may be disposed on the outer wall of the liquid storage chamber 31, and the setting heights of the plurality of third liquid level sensors 36 on the liquid storage chamber 31 may correspond to different preset liquid injection lines. For example, as a specific example, as understood with reference to fig. 2, two third liquid level sensors 36a and 36b may be provided on the outer wall of the liquid storage chamber 31, wherein one third liquid level sensor 36a may be lower than the first liquid level sensor and its set height on the liquid storage chamber 31 may correspond to the liquid level line of the first preset volume, and the other third liquid level sensor 36b may be higher than the first liquid level sensor 33 and the difference in height between the third liquid level sensor 36b and the first liquid level sensor 33 may correspond to the liquid level height of the second preset volume in the liquid storage chamber 31; further, the volume corresponding to the height difference between the first liquid level sensor 33 and one of the third liquid level sensors 36a in the liquid storage chamber 31 may be denoted as V1 ', the volume corresponding to the height difference between the second liquid level sensor 34 and the other of the third liquid level sensors 36b in the liquid storage chamber 31 may be denoted as V2', and the ratio of the first preset volume to V1 'and the ratio of the second preset volume to V2' may be equal, where, in the actual operation, when the second reagent and the water sample to be measured need to be added into the liquid storage chamber 31, the following operations may be included:
1) Switching the input end of the power pump 20 to the second reagent inlet 14b through the multiple valves 16, closing the second stop valve 40b, opening the first stop valve 40a, switching the first three-way valve 50 to the first discharge end 52, starting the power pump 20, pumping the second reagent into the liquid storage cavity 31 of the protection device 30, and stopping the power pump 20 when the liquid level position of the second reagent in the liquid storage cavity 31 reaches the liquid level height corresponding to one of the third liquid level sensors 36 a; switching the input end of the power pump 20 to the water sample inlet 13 to be detected through the multiple valves 16, starting the power pump 20, pumping the water sample to be detected into the liquid storage cavity 31 of the protection device 30, and stopping the power pump 20 when the liquid level in the liquid storage cavity 31 reaches the other third liquid level sensor 36 b;
2) The input end of the power pump 20 is switched to the air inlet 11 through the multiple valves 16, the first stop valve 40a is opened, the first three-way valve 50 is switched to the first discharge end 52, the power pump 20 is started, and the air blowing, stirring and mixing are uniform;
3) Closing the first stop valve 40a, switching the first three-way valve 50 to the first waste liquid end 53, discharging the gas in the line between the protection device 30 and the first three-way valve 50 (during actual operation, the discharging process may be performed by discharging liquid, the specific liquid discharge amount may be flexibly adjusted by a preset time and/or a preset liquid discharge volume, for example, the liquid discharge may be continuously performed to the preset time and/or the preset liquid discharge volume, or the liquid discharge amount may be controlled based on the line capacity and/or the excess liquid flow in the unit time of the power pump), in this example, the liquid may be discharged until the liquid level in the liquid storage chamber 31 is reduced from the liquid level height corresponding to the first liquid level sensor 33 by another third liquid level sensor 36b, and the power pump 20 is turned off;
4) Switching the input end of the power pump 20 to the second reagent inlet 14b through the multiple valves 16, opening the first stop valve 40a, switching the first three-way valve 50 to the first discharge end 52, starting the power pump 20, pumping the second reagent into the liquid storage cavity 31 of the protection device 30, and stopping the power pump 20 when the liquid level position of the second reagent in the liquid storage cavity 31 reaches the liquid level height corresponding to the other third liquid level sensor 36 b; the input end of the power pump 20 is switched to the water sample inlet 13 to be detected through the multiple valves 16, the power pump 20 is started, the water sample to be detected is pumped into the liquid storage cavity 31 of the protection device 30, and the power pump 20 is stopped when the liquid level in the liquid storage cavity 31 reaches the second liquid level sensor 34.
In this process, the protection device 30 may be regarded as a reagent mixing area, and may implement accurate liquid taking through the fixed pipeline length+the liquid level sensor+the power pump, that is, the third liquid level sensor may be used to cooperate with the first liquid level sensor and the second liquid level sensor to ensure that the volume ratio of the second reagent and the water sample to be measured, which are sequentially supplied to the liquid storage cavity, is the same. In addition, in the process, the air can be used for realizing uniform mixing of the second reagent and the water sample to be detected, the pipeline 35 stretches into the liquid storage cavity 31, and the outlet of the pipeline 35 is lower than the first liquid level sensor 33, so that the blowing stirring effect can be further improved.
In some embodiments of the present invention, it is understood with reference to fig. 2 that the specific structure of the protection device 30 is not particularly limited, and those skilled in the art may flexibly select according to actual needs, for example, the protection device 30 may include: at least two balls 31a arranged up and down, the exhaust port being provided at an upper portion of the ball 31a at the top; a first cylindrical pipe 31b, wherein two adjacent spheres 31a are communicated through the first cylindrical pipe 31 b; the second cylindrical tube 31c, the second cylindrical tube 31c is communicated with the sphere 31a at the bottom and is positioned below the sphere 31a, the first liquid level sensor 33 is higher than the second cylindrical tube 31c, the first cylindrical tube 31b and the inner cavity of the sphere 31a are combined to form the liquid storage cavity 31, and the device is not only beneficial to realizing exhaust operation, but also beneficial to realizing mixing of a reagent and a water sample to be detected.
In some embodiments of the present invention, it is understood with reference to fig. 2, and for convenience of understanding the microbial electrochemical sensor testing system according to the embodiments of the present invention, a complete method for facilitating the testing of water quality biotoxicity by the system will be described in detail. The method may include:
(1) Closing the second shut-off valve 40b, opening the first shut-off valve 40a, switching the first three-way valve 50 to the first discharge end 52, switching the input end of the power pump 20 to the first reagent inlet 14a through the plurality of valves 16, and starting the power pump 20 to pump the first reagent into the liquid storage chamber 31; when the second liquid level sensor 34 recognizes that the liquid level in the liquid storage chamber 31 reaches the signal of the preset maximum liquid injection line, the first stop valve 40a is closed.
(2) The first three-way valve 50 is switched to the first waste liquid end 53 to discharge the gas in the pipeline between the protection device 30 and the first three-way valve 50 (in actual operation, the discharge process can be realized by liquid discharge, and the specific liquid discharge amount can be flexibly adjusted by the preset time and/or the preset liquid discharge volume, for example, the liquid discharge can be continuously performed to the preset time and/or the preset liquid discharge volume, or the liquid discharge amount can be controlled based on the pipeline capacity and/or the excess liquid flow in the unit time of the power pump).
(3) Closing the power pump 20, switching the first three-way valve 50 to the first discharge end 52, opening the second stop valve 40b, and switching the second three-way valve 70 to the second waste end 73; the power pump 20 is started to displace the liquid in the microbial electrochemical sensor 60.
(4) Closing the power pump 20, switching the input end of the power pump 20 to the circulating liquid inlet 15 through the plurality of valves 16, and switching the second three-way valve 70 to the second discharge end 72; the power pump 20 is started, and the culture is circulated. When the first liquid level sensor 33 recognizes that the liquid level of the liquid storage cavity 31 reaches a signal of a preset exhaust liquid level line, the power pump is turned off, and the operations of steps (1) - (4) are repeated; when the bubble sensor 80 recognizes a signal that the amount of bubbles in the pipe line between the microbial electrochemical sensor 60 and the second shut-off valve 40b is not lower than a preset critical value, the power pump 20 and the second shut-off valve are closed, and the operation is stopped.
(I) The first stop valve 40a and the second stop valve 40b are closed, the first three-way valve 50 is switched to the first waste liquid end 53, the input end of the power pump 20 is switched to the air inlet 11 through the plurality of valves 16, and the power pump 20 is started to discharge the residual liquid in the liquid storage cavity 31. For example, it is useful to include: (I-1) opening the first shut-off valve 40a and closing the second shut-off valve 40b, switching the first three-way valve 50 to the first waste liquid end 53, and discharging the residual liquid in the liquid storage chamber 31 under the action of gravity; (I-2) closing the first shut-off valve 40a, switching the input of the power pump 20 to the air inlet 11 through the multiple valves 16, starting the power pump 20 to further drain the residual liquid in the liquid storage chamber 31.
(II) closing the power pump 20, opening the first shut-off valve 40a, switching the first three-way valve 50 to the first discharge end 52, switching the input end of the power pump 20 to the pure water inlet 12 through the plurality of valves 16; starting the power pump 20 to clean the liquid storage cavity 31 and the connecting pipeline; when the second level sensor 34 recognizes that the liquid level in the liquid storage chamber 31 reaches the signal of the preset maximum liquid injection line, the power pump 20 is turned off within the preset time, for example, the power pump 20 can be turned off after 2 s.
(III) closing the first shut-off valve 40a, switching the first three-way valve 50 to the first waste side 53, switching the input of the power pump 20 to the air inlet 11 through the multiple valves 16; the power pump 20 is started to discharge the water in the liquid storage cavity 31 and the connecting pipeline.
(5) Closing the second shut-off valve 40b, opening the first shut-off valve 40a, switching the first three-way valve 50 to the first discharge end 52, switching the input end of the power pump 20 to the second reagent inlet 14b through the plurality of valves 16, and starting the power pump 20 to pump the second reagent into the liquid storage chamber 31; when the volume of the second reagent in the liquid storage cavity 31 reaches the first preset volume, the power pump 20 is turned off; switching the input end of the power pump 20 to the water sample inlet 13 to be tested through the plurality of valves 16, and starting the power pump 20 to pump the water sample to be tested into the liquid storage cavity 31; when the second liquid level sensor 34 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset maximum liquid injection line, the power pump 20 is turned off;
(6) Switching the input end of the power pump 20 to the air inlet 11 through a plurality of valves 16, and starting the power pump 20 to perform blowing stirring;
(7) Closing the first shut-off valve 40a, switching the first three-way valve 50 to the first waste liquid end 53, and discharging the gas in the line between the protection device 30 and the first three-way valve 50;
(8) Closing the power pump 20, opening the first stop valve 40a, switching the first three-way valve 50 to the first discharge end 52, switching the input end of the power pump 20 to the second reagent inlet 14b through the plurality of valves 16, and starting the power pump 20 to pump a second reagent of a second preset volume into the liquid storage cavity 31; turning off the power pump 20; switching the input end of the power pump 20 to the water sample inlet 13 to be tested through the plurality of valves 16, and starting the power pump 20 to pump the water sample to be tested into the liquid storage cavity 31; when the second liquid level sensor 34 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset maximum liquid injection line, the power pump 20 is turned off; the volume of the water sample to be detected pumped into the liquid storage cavity 31 in the step (8) is V2, the volume of the water sample to be detected pumped into the liquid storage cavity 31 in the step (5) is V1, and the ratio of the second preset volume to V2 and the ratio of the first preset volume to V1 can be equal.
(9) The input of the power pump 20 is switched to the air inlet 11 through the multiple valves 16, and the power pump 20 is started for blowing stirring.
(10) Closing the power pump 20, switching the first three-way valve 50 to the first discharge end 52, opening the second shut-off valve 40b, and switching the second three-way valve 70 to the second discharge end 72; the power pump 20 is started, the culture is circulated,
wherein: in the step (10), when the first liquid level sensor 33 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset exhaust liquid level line, the power pump is turned off, and the operations of the steps (5) - (10) are repeated; when the bubble sensor 80 recognizes a signal that the amount of bubbles in the pipe line between the microbial electrochemical sensor 60 and the second shut-off valve 40b is not lower than a preset critical value, the power pump 20 and the second shut-off valve are closed, and the operation is stopped.
The method has all the characteristics and effects described in the microbial electrochemical sensor test system, and are not described herein.
In some embodiments of the invention, the microbial electrochemical sensor testing system may further comprise: an automatic control unit (not shown) which may be connected to the plurality of valves 16, the power pump 20, the first cut-off valve 40a, the first liquid level sensor 33, the second liquid level sensor 34, the first three-way valve 50, the bubble sensor 80, the second cut-off valve 40b, and the second three-way valve 70, and adapted to receive the liquid level signals recognized by the first liquid level sensor 33 and the second liquid level sensor 34, the bubble amount signals recognized by the bubble sensor 80, and control based on a preset program: the power pump 20, the first stop valve 40a and the second stop valve 40b are opened and closed, and the ports of the multiple valves 16, the first three-way valve 50 and the second three-way valve 70 are switched, so that automatic running and exhausting of a preset program are realized; the preset program includes a incubation test program and a cleaning program. Further, the control unit may be further connected to the third level sensor 36 and adapted to receive a level signal identified by the third level sensor 36, whereby the control unit is adapted to receive a level signal identified by the third level sensor 36, the first level sensor 33, the second level sensor 34, a bubble amount signal identified by the bubble sensor 80 and control, based on a preset program: the opening and closing of the power pump 20, the first shut-off valve 40a and the second shut-off valve 40b, and the port switching of the multiple valves 16, the first three-way valve 50 and the second three-way valve 70 realize the automatic operation and the exhaust of the preset program. In the existing bubble-removing liquid path system structure, the risk that the bubble-removing capacity is limited and reagent leaks exists generally, and the bubble-removing liquid path system cannot be used for long-term unattended water quality toxicity monitoring.
In some embodiments of the present invention, the kinds of the first three-way valve 50 and the second three-way valve 70 are not particularly limited, and those skilled in the art can flexibly select according to actual needs, for example, can be solenoid valves, etc. independently.
The second aspect of the invention provides a method for testing water quality biotoxicity by using the microbial electrochemical sensor testing system. As understood with reference to fig. 1 or 2, according to an embodiment of the present invention, the method includes:
(1) Closing the second stop valve 40b, opening the first stop valve 40a, switching the first three-way valve 50 to the first discharge end 52, switching the input end of the power pump 20 to the reagent inlet 14 or the water sample inlet 13 to be tested through the plurality of valves 16, starting the power pump 20 to pump the reagent into the liquid storage cavity 31, or respectively pumping the reagent and the water sample to be tested into the liquid storage cavity 31; when the second liquid level sensor 34 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset maximum liquid injection line, the first stop valve 40a is closed;
(2) Switching the first three-way valve 50 to the first waste liquid end 53 to discharge the gas in the pipeline between the protection device 30 and the first three-way valve 50 (in actual operation, the discharge process may be realized by liquid discharge, the specific liquid discharge amount may be flexibly adjusted by a preset time and/or a preset liquid discharge volume, for example, liquid discharge may be continued to a preset time and/or a preset liquid discharge volume, or the liquid discharge amount may be controlled based on the pipeline capacity and/or the excess liquid flow rate in unit time of the power pump);
(3) Closing the power pump 20, switching the first three-way valve 50 to the first discharge end 52, opening the second stop valve 40b, and switching the second three-way valve 70 to the second waste end 73; activating the power pump 20 to displace the liquid in the microbial electrochemical sensor 60;
(4) Closing the power pump 20, switching the input end of the power pump 20 to the circulating liquid inlet 15 through the plurality of valves 16, and switching the second three-way valve 70 to the second discharge end 72; the power pump 20 is started, the culture is circulated,
wherein: in the step (4), when the first liquid level sensor 33 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset exhaust liquid level line, the power pump is turned off, and the operations of the steps (5) - (10) are repeated; when the bubble sensor 80 recognizes a signal that the amount of bubbles in the pipe line between the microbial electrochemical sensor 60 and the second shut-off valve 40b is not lower than a preset critical value, the power pump 20 and the second shut-off valve are closed, and the operation is stopped.
The method for testing the biotoxicity of water quality according to the embodiment of the invention has at least the following advantages: 1. the method can be combined with a protection device to effectively remove bubbles at the front end of the microbial electrochemical sensor before and during the test, has no capacity expansion and bubble discharge capacity, solves the problem that the electric signal is influenced by bubbles, further improves the stability of the microbial electrochemical sensor, prolongs the service life of the microbial electrochemical sensor and realizes the online long-term stable monitoring of the water biotoxicity; 2. the bubble sensor can be used for identifying whether the bubble quantity in a pipeline between the microbial electrochemical sensor and the second stop valve exceeds a preset critical value, judging whether the microbial electrochemical sensor is likely to be disconnected or not based on the identified signal, and stopping the operation if the power pump and the second stop valve are required to be closed so as to prevent the microbial electrochemical sensor from leaking liquid and protect the microbial electrochemical sensor; 3. the liquid level sensor can be used for judging the liquid level position in the liquid storage cavity and judging whether liquid is required to be replenished into the liquid storage cavity or whether the exhaust operation is required to be carried out by combining the protection device or not based on the identification result; 4. the protection device is convenient to clean and change liquid.
In some embodiments of the invention, before performing step (1) and/or after performing step (4) further comprises: draining the residual liquid in the liquid storage chamber 31 and cleaning the liquid storage chamber 31, as understood with reference to fig. 1 or fig. 2, the specific steps may include: (a) Closing the first stop valve 40a and the second stop valve 40b, switching the first three-way valve 50 to the first waste liquid end 53, switching the input end of the power pump 20 to the air inlet 11 through the plurality of valves 16, and starting the power pump 20 to discharge the residual liquid in the liquid storage cavity 31; (b) Closing the power pump 20, opening the first stop valve 40a, switching the first three-way valve 50 to the first discharge end 52, and switching the input end of the power pump 20 to the pure water inlet 12 through the plurality of valves 16; starting the power pump 20 to clean the liquid storage cavity 31 and the connecting pipeline; when the second liquid level sensor 34 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset maximum liquid injection line, the power pump 20 is turned off within the preset time, for example, the power pump 20 can be turned off after 2 s; (c) Closing the first shut-off valve 40a, switching the first three-way valve 50 to the first waste side 53, switching the input of the power pump 20 to the air inlet 11 through the multiple valves 16; the power pump 20 is started to discharge the water in the liquid storage cavity 31 and the connecting pipeline. The foregoing operations are described in detail in the foregoing sections, and are not repeated herein.
In some embodiments of the present invention, as understood with reference to fig. 1 or 2, step (a) may include: (a-1) opening the first shut-off valve 40a and closing the second shut-off valve 40b, switching the first three-way valve 50 to the first waste liquid end 53, and discharging the residual liquid in the liquid storage chamber 31 under the action of gravity; (a-2) closing the first shut-off valve 40a, switching the input of the power pump 20 to the air inlet 11 through the multiple valves 16, and starting the power pump 20 to further discharge the residual liquid in the liquid storage chamber 31. The foregoing operations are described in detail in the foregoing sections, and are not repeated herein.
In some embodiments of the present invention, as understood with reference to fig. 2, a complete method of testing water quality biotoxicity may include: sequentially performing steps (1) - (4), steps (a) - (c) and steps (5) - (10), wherein:
in step (1), the second stop valve 40b is closed, the first stop valve 40a is opened, the first three-way valve 50 is switched to the first discharge end 52, the input end of the power pump 20 is switched to the first reagent inlet 14a through the multiple valves 16, and the power pump 20 is started to pump the first reagent into the liquid storage cavity 31; when the second liquid level sensor 34 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset maximum liquid injection line, the first stop valve 40a is closed;
The steps (5) - (10) comprise:
(5) Closing the second shut-off valve 40b, opening the first shut-off valve 40a, switching the first three-way valve 50 to the first discharge end 52, switching the input end of the power pump 20 to the second reagent inlet 14b through the plurality of valves 16, and starting the power pump 20 to pump the second reagent into the liquid storage chamber 31; when the volume of the second reagent in the liquid storage cavity 31 reaches the first preset volume, the power pump 20 is turned off; switching the input end of the power pump 20 to the water sample inlet 13 to be tested through the plurality of valves 16, and starting the power pump 20 to pump the water sample to be tested into the liquid storage cavity 31; when the second liquid level sensor 34 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset maximum liquid injection line, the power pump 20 is turned off;
(6) Switching the input end of the power pump 20 to the air inlet 11 through a plurality of valves 16, and starting the power pump 20 to perform blowing stirring;
(7) Closing the first shut-off valve 40a, switching the first three-way valve 50 to the first waste liquid end 53, and discharging the gas in the line between the protection device 30 and the first three-way valve 50;
(8) Closing the power pump 20, opening the first stop valve 40a, switching the first three-way valve 50 to the first discharge end 52, switching the input end of the power pump 20 to the second reagent inlet 14b through the plurality of valves 16, and starting the power pump 20 to pump a second reagent of a second preset volume into the liquid storage cavity 31; turning off the power pump 20; switching the input end of the power pump 20 to the water sample inlet 13 to be tested through the plurality of valves 16, and starting the power pump 20 to pump the water sample to be tested into the liquid storage cavity 31; when the second liquid level sensor 34 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset maximum liquid injection line, the power pump 20 is turned off; the volume of the water sample to be detected pumped into the liquid storage cavity 31 in the step (8) is V2, the volume of the water sample to be detected pumped into the liquid storage cavity 31 in the step (5) is V1, and the ratio of the second preset volume to V2 and the ratio of the first preset volume to V1 can be equal.
(9) The input of the power pump 20 is switched to the air inlet 11 through the multiple valves 16, and the power pump 20 is started for blowing stirring.
(10) Closing the power pump 20, switching the first three-way valve 50 to the first discharge end 52, opening the second shut-off valve 40b, and switching the second three-way valve 70 to the second discharge end 72; the power pump 20 is started, the culture is circulated,
wherein: in the step (10), when the first liquid level sensor 33 recognizes that the liquid level of the liquid storage cavity 31 reaches the signal of the preset exhaust liquid level line, the power pump is turned off, and the operations of the steps (5) - (10) are repeated; when the bubble sensor 80 recognizes a signal that the amount of bubbles in the pipe line between the microbial electrochemical sensor 60 and the second shut-off valve 40b is not lower than a preset critical value, the power pump 20 and the second shut-off valve are closed, and the operation is stopped.
The beneficial effects of the above operations are described in detail in the foregoing sections, and are not described here again.
In some embodiments of the present invention, two third liquid level sensors 36 may be disposed on the outer wall of the liquid storage chamber 31, wherein one third liquid level sensor 36a is disposed below the first liquid level sensor 33 and the disposed height on the liquid storage chamber 3 corresponds to the liquid level line of the first preset volume, and the other third liquid level sensor 36b is disposed above the first liquid level sensor 33 and the height difference between the third liquid level sensor 36b and the first liquid level sensor 33 corresponds to the liquid level height of the second preset volume in the liquid storage chamber 31. The embodiments of this scheme have been illustrated in the previous sections and will not be described here again.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.
Claims (10)
1. A microbial electrochemical sensor testing system, comprising:
The device comprises an air inlet, a pure water inlet, a water sample inlet to be tested, at least one reagent inlet, a circulating liquid inlet and a plurality of valves;
the input end of the power pump is switchably connected with the air inlet, the pure water inlet, the water sample inlet to be tested, the reagent inlet and the circulating liquid inlet through the plurality of valves;
the protection device comprises a liquid storage cavity which can be arranged in a sealing manner, an exhaust port, a second liquid level sensor and a first liquid level sensor are sequentially arranged in the liquid storage cavity from top to bottom, the exhaust port is connected with a first stop valve, the arrangement height of the first liquid level sensor on the liquid storage cavity corresponds to a preset exhaust liquid level line, and the arrangement height of the second liquid level sensor on the liquid storage cavity corresponds to a preset maximum liquid injection line; the inlet of the liquid storage cavity is connected with the output end of the power pump through a pipeline, the pipeline stretches into the liquid storage cavity, and the outlet of the pipeline is lower than the first liquid level sensor;
the first three-way valve comprises a first public end, a first discharge end and a first waste liquid end, and the first public end is connected with an outlet of the liquid storage cavity;
The liquid inlet of the microbial electrochemical sensor is connected with the first discharge end;
the second three-way valve comprises a second public end, a second discharge end and a second waste liquid end, the second public end is connected with a liquid outlet of the microbial electrochemical sensor through a second stop valve, a bubble sensor is arranged between the second stop valve and the liquid outlet of the microbial electrochemical sensor, and the second discharge end is connected with the circulating liquid inlet.
2. The system of claim 1, wherein at least one of the following conditions is satisfied:
the at least one reagent inlet comprises a first reagent inlet and a second reagent inlet, wherein the first reagent is suitable for being used as electrolyte in the microbial electrochemical sensor, and the second reagent is suitable for being mixed with a water sample to be detected for water toxicity detection;
the first liquid level sensor and the second liquid level sensor are arranged on the outer wall of the liquid storage cavity;
the bubble sensor is an ultrasonic sensor;
the liquid storage device is characterized in that at least one third liquid level sensor is arranged on the outer wall of the liquid storage cavity, the third liquid level sensor is lower than the second liquid level sensor, and the height of the third liquid level sensor on the outer wall of the liquid storage cavity is adjustable.
3. The system according to claim 2, wherein a plurality of third liquid level sensors are arranged on the outer wall of the liquid storage cavity at intervals up and down, and the setting heights of the plurality of third liquid level sensors on the liquid storage cavity correspond to different preset liquid injection lines.
4. The system of claim 1, wherein the protection device comprises:
at least two spheres arranged up and down, the exhaust port being provided at the upper part of the sphere at the top;
the two adjacent spheres are communicated through the first cylindrical pipe;
the second column tube, the second column tube with be located the bottom the spheroid intercommunication sets up and is located this spheroid below, first level sensor is higher than the second column tube sets up, the second column tube with first column tube with spheroidal inner chamber combination forms the stock solution chamber.
5. The system of any one of claims 1-4, further comprising:
the automatic control unit is connected with the multiple valves, the power pump, the first stop valve, the first liquid level sensor, the second liquid level sensor, the first three-way valve, the bubble sensor, the second stop valve and the second three-way valve, is suitable for receiving liquid level signals identified by the first liquid level sensor and the second liquid level sensor and bubble quantity signals identified by the bubble sensor, and controls based on a preset program: the power pump, the first stop valve and the second stop valve are opened and closed, and the ports of the multiple valves, the first three-way valve and the second three-way valve are switched, so that the automatic operation and the exhaust of the preset program are realized; the preset program comprises a culture test program and a cleaning program.
6. A method of testing water quality biotoxicity using the system of any one of claims 1-5, comprising:
(1) Closing a second stop valve, opening a first stop valve, switching a first three-way valve to a first discharge end, switching an input end of a power pump to a reagent inlet or a water sample inlet to be detected through a plurality of valves, and starting the power pump to pump the reagent into a liquid storage cavity or respectively pump the reagent and the water sample to be detected into the liquid storage cavity; when the second liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset maximum liquid injection line, the first stop valve is closed;
(2) Switching the first three-way valve to a first waste liquid end, and discharging gas in a pipeline between the protection device and the first three-way valve;
(3) Closing the power pump, switching the first three-way valve to the first discharge end, opening the second stop valve, and switching the second three-way valve to the second waste liquid end; starting the power pump to replace the liquid in the microbial electrochemical sensor;
(4) Closing the power pump, switching the input end of the power pump to a circulating liquid inlet through the plurality of valves, and switching the second three-way valve to a second discharge end; starting the power pump, circularly culturing,
Wherein: in the step (4), when the first liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset exhaust liquid level line, the power pump is turned off, and the operations of the steps (1) - (4) are repeated; and when the bubble sensor recognizes that the bubble quantity in the pipeline between the microbial electrochemical sensor and the second stop valve is not lower than a preset critical value, closing the power pump and the second stop valve, and stopping operation.
7. The method for testing the biotoxicity of water according to claim 6, wherein before performing step (1) and/or after performing step (4) further comprises: the method for discharging the residual liquid in the liquid storage cavity and cleaning the liquid storage cavity comprises the following specific steps:
(a) Closing the first stop valve and the second stop valve, switching the first three-way valve to the first waste liquid end, switching the input end of the power pump to an air inlet through the plurality of valves, and starting the power pump to discharge residual liquid in the liquid storage cavity;
(b) Closing the power pump, opening the first stop valve, switching the first three-way valve to the first discharge end, and switching the input end of the power pump to the pure water inlet through the plurality of valves; starting the power pump to clean the liquid storage cavity and the connecting pipeline; when the second liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset maximum liquid injection line, the power pump is turned off within a preset time;
(c) Closing the first stop valve, switching the first three-way valve to the first waste liquid end, and switching the input end of the power pump to the air inlet through the plurality of valves; and starting the power pump to discharge the water in the liquid storage cavity and the connecting pipeline.
8. The method of testing water quality biotoxicity of claim 7, wherein step (a) comprises:
(a-1) opening the first shut-off valve and closing the second shut-off valve, switching the first three-way valve to the first waste liquid end, and discharging residual liquid in the liquid storage cavity under the action of gravity;
(a-2) closing the first shut-off valve, switching the input of the power pump to the air inlet through the plurality of valves, and starting the power pump to further drain the residual liquid in the liquid storage chamber.
9. The method for testing the biotoxicity of water according to any one of claims 6 to 8, comprising: sequentially performing steps (1) - (4), steps (a) - (c) and steps (5) - (11), wherein:
in the step (1), the second stop valve is closed, the first stop valve is opened, the first three-way valve is switched to the first discharge end, the input end of the power pump is switched to a first reagent inlet through the plurality of valves, and the power pump is started to pump a first reagent into the liquid storage cavity; when the second liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset maximum liquid injection line, the first stop valve is closed;
The steps (5) - (11) comprise:
(5) Closing the second stop valve, opening the first stop valve, switching the first three-way valve to the first discharge end, switching the input end of the power pump to a second reagent inlet through the plurality of valves, and starting the power pump to pump a second reagent into the liquid storage cavity; when the volume of the second reagent in the liquid storage cavity reaches a first preset volume, the power pump is turned off; switching the input end of the power pump to a water sample inlet to be detected through the plurality of valves, and starting the power pump to pump the water sample to be detected into the liquid storage cavity; when the second liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset maximum liquid injection line, the power pump is turned off;
(6) Switching the input end of the power pump to the air inlet through the multiple valves, and starting the power pump to blow and stir;
(7) Closing the first stop valve, switching the first three-way valve to the first waste liquid end, and discharging gas in a pipeline between the protection device and the first three-way valve;
(8) Closing the power pump, opening the first stop valve, switching the first three-way valve to the first discharge end, switching the input end of the power pump to the second reagent inlet through the plurality of valves, and starting the power pump to pump a second reagent with a second preset volume into the liquid storage cavity; closing the power pump; switching the input end of the power pump to the water sample inlet to be detected through the plurality of valves, and starting the power pump to pump the water sample to be detected into the liquid storage cavity; when the second liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset maximum liquid injection line, the power pump is turned off; the volume of the water sample to be detected pumped into the liquid storage cavity in the step (8) is V2, the volume of the water sample to be detected pumped into the liquid storage cavity in the step (5) is V1, and the ratio of the second preset volume to V2 is equal to the ratio of the first preset volume to V1;
(9) Switching the input end of the power pump to an air inlet through the multiple valves, and starting the power pump to blow and stir;
(10) Closing the power pump, switching the first three-way valve to the first discharge end, opening the second stop valve, and switching the second three-way valve to the second discharge end; starting the power pump, circularly culturing,
wherein: in the step (10), when the first liquid level sensor recognizes that the liquid level of the liquid storage cavity reaches a signal of a preset exhaust liquid level line, the power pump is turned off, and the operations of the steps (5) - (10) are repeated; and when the bubble sensor recognizes that the bubble quantity in the pipeline between the microbial electrochemical sensor and the second stop valve is not lower than a preset critical value, closing the power pump and the second stop valve, and stopping operation.
10. The method according to claim 9, wherein two third liquid level sensors are disposed on the outer wall of the liquid storage cavity, one of the third liquid level sensors is lower than the liquid level line of the first preset volume and the disposed height on the liquid storage cavity corresponds to the liquid level height of the second preset volume in the liquid storage cavity, and the other third liquid level sensor is higher than the first liquid level sensor and the height difference between the third liquid level sensor and the first liquid level sensor corresponds to the liquid level height of the second preset volume.
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