CN116973170A - Sampling mechanism and sampling method of protein analyzer - Google Patents

Sampling mechanism and sampling method of protein analyzer Download PDF

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Publication number
CN116973170A
CN116973170A CN202310783148.6A CN202310783148A CN116973170A CN 116973170 A CN116973170 A CN 116973170A CN 202310783148 A CN202310783148 A CN 202310783148A CN 116973170 A CN116973170 A CN 116973170A
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CN
China
Prior art keywords
sampling
sampling needle
lifting
needle
connecting rod
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Pending
Application number
CN202310783148.6A
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Chinese (zh)
Inventor
付健
杜津舟
胡亚林
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Chongqing Biostec Biotechnology Co ltd
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Chongqing Biostec Biotechnology Co ltd
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Priority to CN202310783148.6A priority Critical patent/CN116973170A/en
Publication of CN116973170A publication Critical patent/CN116973170A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices

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  • Life Sciences & Earth Sciences (AREA)
  • Hydrology & Water Resources (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention belongs to the technical field of sampling, and particularly discloses a sampling method of a protein analyzer, which uses a sampling mechanism for sampling, wherein the sampling mechanism comprises a sampling needle, and the sampling needle descends in two sections, and comprises the following steps: s1, controlling a sampling needle to move downwards to be above the liquid level, and staying for a certain time, wherein the air pressure in a needle tube of the sampling needle is recovered; s2, controlling the sampling needle to continuously move downwards and insert into the liquid level to suck samples. The sampling mechanism comprises a frame and a sampling needle mechanism, wherein the sampling needle mechanism comprises a sampling needle, a rotating arm, a connecting rod and a liquid path system for providing positive and negative air pressure for the sampling needle, the rotating arm is connected with the connecting rod, and the sampling needle is connected with one end of the rotating arm; the frame is provided with a rotary driving unit for driving the sampling needle mechanism to rotate and a lifting driving unit for driving the sampling needle mechanism to move up and down. The invention can effectively improve the sample suction precision of the sampling needle.

Description

Sampling mechanism and sampling method of protein analyzer
Technical Field
The invention relates to the technical field of sampling, in particular to a sampling mechanism and a sampling method of a protein analyzer.
Background
At present, in a protein analyzer, a sample and a reagent are required to be mixed with each other and then subjected to detection analysis, and in the process, in order to improve the operation efficiency, a sampling mechanism is generally arranged, and the operation of collecting the sample is realized by driving a sampling needle in the sampling mechanism to move up and down.
The sampling is realized by a plunger pump, the plunger pump is provided with a controllable electric variable-volume cavity, the cavity is filled with liquid, and a liquid path is communicated with the variable-volume cavity, so that the volume change of the liquid path connected with the cavity is realized, and under the action of atmospheric pressure, when the variable-volume cavity of the plunger pump is increased, the sample suction is realized; when the variable volume cavity of the plunger pump is reduced, the discharging is realized.
However, in general, when the sampling needle moves at a high speed, particularly when the sampling needle descends at a high speed, the gas in the needle tube of the sampling needle is extruded, the gas pressure is increased, when the plunger pump is used for positive displacement and sucking liquid after the sampling needle is inserted into the liquid level, the extruded gas can influence the accuracy of sucking the sample, and the accuracy of sucking the sample is seriously affected because the sucking amount is small, usually as small as 2 ul.
Disclosure of Invention
The invention provides a sampling device and a sampling method of a protein analyzer, and aims to improve sampling accuracy of a sampling needle.
The invention is realized by the following technical scheme: a sampling method of a protein analyzer uses a sampling mechanism to sample, the sampling mechanism comprises a sampling needle, the sampling needle descends in two sections to sample, and the sampling method comprises the following steps:
s1, controlling a sampling needle to move downwards to be above the liquid level, and staying for more than 200ms, wherein the air pressure in a needle tube of the sampling needle is recovered to the atmospheric pressure;
s2, controlling the sampling needle to continuously move downwards and insert into the liquid level to suck samples.
Compared with the prior art, the invention has the following advantages and beneficial effects:
according to the invention, the needle point of the sampling needle is stopped for a period of time before reaching the liquid, the first section of descending movement is completed by controlling the sampling needle, then after the air pressure in the needle tube of the sampling needle is recovered, the sampling needle is controlled to continuously move downwards to below the liquid level to realize the second section of movement of the sampling needle, so that after the sampling needle reaches the liquid filled with the sample through the movement of the two ends, a plunger pump connected with a liquid path of the sampling needle can accurately transfer the pressure to the needle point of the sampling needle through variable capacity sample suction, and the sample suction precision can be effectively improved.
Further, the sampling needle moves downwards to above the liquid surface at a first speed in S1, and the sampling needle moves downwards and inserts into the liquid surface at a second speed in S2 for sample suction, wherein the first speed is higher than the second speed.
The beneficial effects are that: because the machine has technical requirements on the detection speed, the sampling needle can move quickly in general), the sampling needle stops in the high-speed descending process, the air pressure in the needle tube can be restored to the atmospheric pressure, and when the needle tube is inserted into the liquid level to suck samples at a constant speed, the variable capacity of the plunger pump is accurately transferred to the needle point, so that the sampling precision, particularly the sampling precision of micro samples, is improved.
The sampling mechanism of the protein analyzer comprises a frame and a sampling needle mechanism, wherein the sampling needle mechanism comprises a sampling needle, a rotating arm, a connecting rod and a liquid path system for providing positive and negative air pressure for the sampling needle, the rotating arm is connected with the connecting rod, and the sampling needle is connected with one end of the rotating arm; the frame is provided with a rotary driving unit for driving the sampling needle mechanism to rotate and a lifting driving unit for driving the sampling needle mechanism to move up and down.
The beneficial effects are that: in this scheme, can drive the swinging boom through rotary drive unit in the sampling needle mechanism and drive the sampling needle and produce rotary motion, be convenient for shift the sample of absorbing, and lift drive unit can drive the connecting rod and drive the sampling needle elevating movement on swinging boom to realize inserting the liquid level and absorb the sample or draw in the liquid after absorbing the sample.
Further, the lifting driving unit comprises a lifting belt, a lifting motor and two lifting belt wheels, an output shaft of the lifting motor is connected with one of the lifting belt wheels, and the lifting belt is positioned on the two lifting belt wheels; the lifting sliding rail is arranged on the frame in a vertical mode, a lifting sliding block is connected to the lifting sliding rail in a sliding mode, the lifting sliding block is connected with the lifting belt, and one end of the connecting rod is connected with the lifting sliding block.
The beneficial effects are that: in this scheme drive lifting belt cyclic motion through starting elevator motor to drive the lifting slide who is connected with it and slide along the lift slide rail, and then realize elevating movement, lift drive unit simple structure in this scheme, and operate steadily.
Further, the rotary driving unit comprises a rotary motor, a rotary belt, a driving belt wheel and a driven belt wheel, wherein the driving belt wheel is connected with an output shaft of the rotary motor, the rotary belt is positioned on the driving belt wheel and the driven belt wheel, the driven belt wheel is coaxially connected with the connecting rod, and the connecting rod can vertically move along the driven belt wheel.
The beneficial effects are that: in this scheme in the rotary drive unit drive rotary belt cyclic motion's in-process through the rotating electrical machines, drive driven pulleys and rotate to drive the connecting rod with driven pulleys coaxial coupling and rotate, and then realize the rotation of sampling needle, the connecting rod can be along driven pulleys vertical movement in this scheme in addition, can carry out smooth elevating movement when being convenient for lift drive unit drive connecting rod vertical movement like this.
Further, coaxial coupling has the connecting axle in the driving pulley, vertical connection has the flat key on the connecting rod, the through-hole has been seted up to the connecting axle is coaxial, vertical setting's keyway has been seted up to the through-hole inner wall of connecting axle, the connecting rod passes through the flat key is connected with the keyway cooperation of connecting axle, just the flat key on the connecting rod can be in vertical slip in the keyway.
The beneficial effects are that: the flat key of connecting rod in this scheme can thereby guarantee in the keyway of connecting axle that lift drive unit can be smooth drive the connecting rod and carry out normal elevating movement, and the flat key is located the keyway simultaneously, can make the connecting rod receive connecting axle circumference direction's restriction like this, can drive the connecting rod and rotate when driving pulley drives the connecting axle pivoted to realize the normal rotary motion of sampling needle.
Further, the driven pulley has a diameter greater than the diameter of the driving pulley.
The beneficial effects are that: the arrangement can play a role in decelerating, so that the rotary motion of the sampling needle is more stable.
Further, the liquid level detecting device comprises a controller and a liquid level detecting unit, wherein the liquid level detecting unit, the rotary driving unit and the lifting driving unit are electrically connected with the controller, and the liquid level detecting unit is used for detecting whether the sampling needle is in contact with the liquid level.
The beneficial effects are that: in this scheme, through the action of controller automatic control rotation drive unit and lift drive unit, more automatic, liquid level detection unit can detect whether sampling needle and liquid level contact in this scheme to give the controller after the signal of being convenient for gathers, make the sampling of controller control sampling needle draw the action.
Further, the swinging boom includes backup pad and arm, the backup pad with bracing piece fixed connection, the arm with backup pad horizontal slip connection, the sampling needle with the arm is connected, be equipped with the drive in the backup pad the power pack of arm horizontal slip.
The beneficial effects are that: the rotating arm comprises a supporting plate and a sliding arm, the sliding arm is in sliding connection with the supporting plate, and the horizontal position of the sampling needle can be changed through the sliding process of the driving sliding arm along the supporting plate, so that the operation of sucking samples under different conditions is adapted, the sampling range is wider, and the application range is improved.
Further, the power unit includes horizontal migration motor, horizontal belt and two horizontal pulleys, horizontal belt connects two on the horizontal pulley, the backup pad top is connected with the guide rail, the bottom of arm is connected with the guide block, the guide block with guide rail sliding fit, just the guide block with horizontal belt connects.
The beneficial effects are that: the power unit occupies a small installation volume in the scheme, and the driving mode is simple and stable.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. In the drawings:
FIG. 1 is a perspective view of a sampling mechanism embodiment 1 of a protein analyzer according to the present invention;
FIG. 2 is a front view of a sampling mechanism of a protein analyzer according to embodiment 2 of the present invention;
FIG. 3 is a schematic diagram showing the movement of the sampling needle in the sampling mechanism of embodiment 2 of a protein analyzer according to the present invention.
In the drawings, the reference numerals and corresponding part names:
the device comprises a frame 1, a connecting rod 2, a rotating arm 3, a supporting plate 301, a sliding arm 302, a sampling needle 4, a lifting motor 5, a rotating motor 6, a lifting sliding rail 7, a lifting sliding block 8, a lifting belt 9, a lifting belt wheel 901, a lifting pressing block 10, a driving belt wheel 11, a driven belt wheel 12, a connecting shaft 121, a rotating belt 13, a guide rail 15, a guide block 16, a horizontal moving motor 17, a horizontal pressing block 18, a horizontal belt 19, a horizontal belt wheel 20, an injection pump 21 and a liquid level detection unit 22.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In this embodiment 1, a sampling method of a protein analyzer is provided, in which a sampling mechanism is used to sample, the sampling mechanism includes a sampling needle 4, and the sampling needle 4 descends in two sections to sample, specifically: the method comprises the following steps:
s1, controlling the sampling needle 4 to move downwards to be above the liquid level and stay for a certain time, wherein the stay time is more than 200ms in the embodiment, and the air pressure in the needle tube of the sampling needle 4 is recovered to the atmospheric pressure;
s2, controlling the sampling needle 4 to continuously move downwards and insert the liquid level to suck samples.
In this embodiment, the sampling needle 4 moves downward to above the liquid surface at a first speed in S1, the sampling needle 4 moves downward at a second speed in S2 and is inserted into the liquid surface to suck samples, the first speed is greater than the second speed, and in this embodiment, the sampling needle 4 moves downward at a uniform speed of 1/5 of the first speed in S2.
As shown in fig. 1, the embodiment also discloses a sampling mechanism in the above sampling method, specifically: the sampling mechanism of the protein analyzer comprises a frame 1 and a sampling needle mechanism, wherein the sampling needle mechanism in the embodiment comprises a sampling needle 4, a rotating arm 3, a connecting rod 2 and a liquid path system for providing positive and negative air pressure for the sampling needle 4, one end of the rotating arm 3 is fixedly connected with the connecting rod 2 through a bolt or a screw, and the sampling needle 4 is connected with the other end of the rotating arm 3.
The frame 1 is provided with a rotary driving unit for driving the sampling needle mechanism to rotate and a lifting driving unit for driving the sampling needle mechanism to move up and down. Specific: the lifting driving unit comprises a lifting belt 9, a lifting motor 5 and two lifting belt pulleys 901, an output shaft of the lifting motor 5 is connected with one of the lifting belt pulleys 901, the two lifting belt pulleys 901 are vertically and oppositely arranged, and the lifting belt 9 is positioned on the two lifting belt pulleys 901; the frame 1 is provided with a vertically arranged lifting slide rail 7, the lifting slide rail 7 is connected with a lifting slide block 8 in a sliding manner, the lifting slide block 8 is connected with a lifting belt 9, in the embodiment, the lifting slide block 8 is connected with a lifting press block 10, the lifting belt 9 is positioned between the lifting slide block 8 and the lifting press block 10, and the lifting belt 9 is fastened between the lifting slide block 8 and the lifting press block 10 by fixing the lifting slide block 10. One end of the connecting rod 2 is connected with the lifting slide block 8, so that the lifting motor 5 drives the lifting belt 9 to circularly move through starting the lifting motor 5, the lifting slide block 8 is driven to move in a lifting mode, and then the connecting rod 2, the rotating arm 3 and the collecting needle are driven to move in a lifting mode, so that sampling is facilitated.
The rotary driving unit in this embodiment includes rotating electrical machines 6, rotating belt 13, driving pulley 11 and driven pulley 12, and the diameter of driven pulley 12 is greater than the diameter of driving pulley 11 in this embodiment, and driving pulley 11 and the output shaft of rotating electrical machines 6, rotating belt 13 are located driving pulley 11 and driven pulley 12, and driven pulley 12 and connecting rod 2 coaxial coupling, and connecting rod 2 can be along driven pulley 12 vertical movement, and specifically: the connecting shaft 121 is coaxially connected in the driving pulley 11, the flat key is vertically connected to the connecting rod 2, the through hole is coaxially formed in the connecting shaft 121, the vertically arranged key groove is formed in the inner wall of the through hole of the connecting shaft 121, the key groove is of a through groove structure, the connecting rod 2 is matched and connected with the key groove of the connecting shaft 121 through the flat key, so that the connecting rod 2 can rotate along with the connecting shaft 121, the flat key on the connecting rod 2 can vertically slide in the key groove, and the connecting rod 2 can move up and down along the lifting sliding rail 7.
The embodiment also comprises a controller and a liquid level detection unit, wherein the liquid level detection unit, the rotary driving unit and the lifting driving unit are all electrically connected with the controller, the liquid level detection unit is used for detecting whether the sampling needle 4 is in contact with the liquid level or not, the liquid level detection unit comprises a liquid level probe, the liquid level detection unit starts to suck samples after detecting that the sampling needle 4 is in contact with the liquid level and then falls into the set distance of the controller, and the smoothness and the accuracy of sucking the samples are ensured.
The specific implementation process is as follows:
in this embodiment, the movement of the rotary driving unit and the lifting driving unit is controlled by the controller, the lifting driving unit drives the sampling needle 4 to move up and down to perform sampling, the rotary driving unit drives the sampling needle 4 to rotate to transfer samples, the sampling needle 4 moves in two sections in the process of descending into the sample bottle to perform sampling, the first section of movement firstly descends to the position above the liquid surface of the sample bottle rapidly, after the first section of movement stays for a period of time, after the pressure in the inner tube of the sampling needle 4 is stable, the second section of movement is performed, the second section of movement is uniformly inserted into the liquid of the sample bottle to perform sample sucking operation, and the sampling needle is driven to move upwards from the sample bottle to transfer the collected samples after sample sucking is completed.
As shown in fig. 2 and 3, embodiment 2 differs from embodiment 1 in that: the rotating arm 3 comprises a supporting plate 301 and a sliding arm 302, the supporting plate 301 is fixedly connected with the supporting rod through bolts or screws, the sliding arm 302 is horizontally and slidably connected with the supporting plate 301, the sampling needle 4 is connected with the sliding arm 302, and a power unit for driving the sliding arm 302 to horizontally slide is arranged on the supporting plate 301.
In this embodiment, the power unit includes horizontal movement motor 17, horizontal belt 19 and two horizontal pulleys 20, horizontal belt 19 connects on two horizontal pulleys 20, backup pad 301 top is connected with guide rail 15, the bottom of arm 302 is connected with guide block 16, guide block 16 and guide rail 15 sliding fit, and guide block 16 is connected with horizontal belt 19, be connected with horizontal briquetting 18 on the guide block 16 in this embodiment, horizontal belt 19 is located between horizontal briquetting 18 and the guide block 16, fix horizontal belt 19 between the two through fixed guide block 16 and horizontal briquetting 18, can drive guide block 16 and arm 302 and produce the removal when horizontal belt 19 removes like this.
In this embodiment, the syringe pump 21 is connected to the liquid path system of the sampling needle 4 through a pipeline, and in combination with the illustration in fig. 3, the sampling needle 4 in this embodiment not only can move up and down and rotate, but also can move horizontally, so that the collection range of the sampling needle 4 can be increased, the application range is enlarged, and the practicability is stronger.
The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the invention, and is not meant to limit the scope of the invention, but to limit the invention to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the invention.

Claims (10)

1. A sampling method of a protein analyzer uses a sampling mechanism to sample, the sampling mechanism comprises a sampling needle, and the sampling mechanism is characterized in that the sampling needle descends in two sections to sample, and the sampling method comprises the following steps:
s1, controlling a sampling needle to move downwards to be above the liquid level, and staying for more than 200ms, wherein the air pressure in a needle tube of the sampling needle is recovered to the atmospheric pressure;
s2, controlling the sampling needle to continuously move downwards and insert into the liquid level to suck samples.
2. The method of claim 1, wherein the sampling needle is moved downward above the liquid surface at a first speed in S1 and the sampling needle is moved downward and inserted into the liquid surface at a second speed in S2 for sampling, the first speed being greater than the second speed.
3. The sampling mechanism of the protein analyzer comprises a frame and a sampling needle mechanism, and is characterized by comprising a sampling needle, a rotating arm, a connecting rod and a liquid path system for providing positive and negative air pressure for the sampling needle, wherein the rotating arm is connected with the connecting rod, and the sampling needle is connected with one end of the rotating arm; the frame is provided with a rotary driving unit for driving the sampling needle mechanism to rotate and a lifting driving unit for driving the sampling needle mechanism to move up and down.
4. The sampling mechanism of a protein analyzer of claim 1, wherein the lifting drive unit comprises a lifting belt, a lifting motor and two lifting pulleys, an output shaft of the lifting motor is connected with one of the lifting pulleys, and the lifting belt is positioned on the two lifting pulleys; the lifting sliding rail is arranged on the frame in a vertical mode, a lifting sliding block is connected to the lifting sliding rail in a sliding mode, the lifting sliding block is connected with the lifting belt, and one end of the connecting rod is connected with the lifting sliding block.
5. The sampling mechanism of claim 4, wherein the rotary drive unit comprises a rotary motor, a rotary belt, a driving pulley and a driven pulley, the driving pulley is connected with an output shaft of the rotary motor, the rotary belt is positioned on the driving pulley and the driven pulley, the driven pulley is coaxially connected with the connecting rod, and the connecting rod can move vertically along the driven pulley.
6. The sampling mechanism of a protein analyzer according to claim 5, wherein a connecting shaft is coaxially connected in the driving pulley, a flat key is vertically connected on the connecting rod, a through hole is coaxially formed in the connecting shaft, a vertically arranged key slot is formed in the inner wall of the through hole of the connecting shaft, the connecting rod is connected with the key slot of the connecting shaft in a matched manner through the flat key, and the flat key on the connecting rod can vertically slide in the key slot.
7. The sampling mechanism of claim 5, wherein the driven pulley has a diameter greater than the diameter of the driving pulley.
8. The sampling mechanism of a protein analyzer of claim 1, further comprising a controller, a liquid level detection unit, wherein the liquid level detection unit, the rotary drive unit and the elevation drive unit are all electrically connected to the controller, and wherein the liquid level detection unit is configured to detect whether the sampling needle is in contact with a liquid level.
9. The sampling mechanism of any one of claims 3 to 8, wherein the rotating arm comprises a support plate and a sliding arm, the support plate is fixedly connected with the support plate, the sliding arm is horizontally and slidably connected with the support plate, the sampling needle is connected with the sliding arm, and a power unit for driving the sliding arm to horizontally slide is arranged on the support plate.
10. The sampling mechanism of claim 7, wherein the power unit comprises a horizontal moving motor, a horizontal belt and two horizontal pulleys, the horizontal belt is connected to the two horizontal pulleys, the top end of the supporting plate is connected with a guide rail, the bottom of the sliding arm is connected with a guide block, the guide block is in sliding fit with the guide rail, and the guide block is connected with the horizontal belt.
CN202310783148.6A 2023-06-29 2023-06-29 Sampling mechanism and sampling method of protein analyzer Pending CN116973170A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310783148.6A CN116973170A (en) 2023-06-29 2023-06-29 Sampling mechanism and sampling method of protein analyzer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310783148.6A CN116973170A (en) 2023-06-29 2023-06-29 Sampling mechanism and sampling method of protein analyzer

Publications (1)

Publication Number Publication Date
CN116973170A true CN116973170A (en) 2023-10-31

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310783148.6A Pending CN116973170A (en) 2023-06-29 2023-06-29 Sampling mechanism and sampling method of protein analyzer

Country Status (1)

Country Link
CN (1) CN116973170A (en)

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