CN1169578C - Beaver thymosin injection and its prepn - Google Patents

Abstract

The present invention relates to a medical beaver thymosin injection which belongs to the technical field of medical products containing the thymus from feeding animals. The medical beaver thymosin injection is prepared by extracting a polypeptide material from the thymus of a beaver, the thymosin dialysate has high activity, the molecular weight is smaller than 10000, and the molecular weight of a main component is smaller than 6000. The content of a thymosin polypeptide is from 7 to 10 milligrams / milliliter, the mean value of the E-rosette activity determination is from 25 to 28%, and the yield coefficient is from 7 to 10 grams of thymosin / kilogram. The preparation method comprises: thymus extraction, homogenization, heating, foreign protein elimination, propanone deposition, sectional salting out, desalinization, drying, ultrafiltration and preparation.

Description

Castor otter medicine thymosin injection and manufacture method thereof

The invention belongs to the medical supplies technical field that contains from the nurture animal thymus, relate to a kind of castor otter medicine thymosin injection.

It is the cluster polypeptides matter that the nurture animal thymus produces that thymosin has another name called thymosin, has control and regulates the effect that animal immune reacts, and has been used to treat immunodeficiency and panimmunity dysfunction disease at present.The raw material of extraction thymosin commonly used is calf thymus and piglet thymus, because raw material is limited, causes cost high, makes thymosin become well sold and in short supply medicine in the clinical practice.1993 9 railway platforms of Chinese invention patent communique disclosed one piece " preparation method of human placental thymosin " on the 22nd, and (publication number: CN1076361A) patent application, the method for preparing thymosin with the thymic tissue of the normal fetus at 5-8 monthly age has been introduced in this patent application.Adopt this kind method to prepare thymosin obviously, its raw material sources are more limited, and its manufacturing cost is higher, and it is applied is very difficult.The main component of the thymic extract of general mammal such as cattle, pig etc. is thymic hyperplasia element, thymosin, thymic humoral factor, serum thymic factor, LS element.But these thymosin molecular weight that extract are big, active low, and result of use is poor.

The objective of the invention is to provide a kind of active height of injection with aboundresources, preparation, avirulence, clinical practice medical thymosin injection of castor otter and manufacture method thereof safely and effectively.

The castor otter has another name called coypu, castor, beaver, is herbvore nurture animal.The easy artificial cultivation of castor otter, the breeding potential height, the artificial cultivation cost is low, and at present, in all parts of the country all have a large amount of artificial cultivation as Shandong Province, Heilongjiang Province, Hebei province, Hunan Province and other places, is mainly used in meat.Therefore, extract thymosin with castor otter thymus, resource is very abundant.

The objective of the invention is to realize with the following methods.Castor otter medicine thymosin injection is the cluster polypeptides matter that extracts from castor otter thymus, its thymosin dialysate is active high, its molecular weight is less than 10000, and main component thymic hyperplasia element, thymosin, thymic humoral factor, serum thymic factor, the plain molecular weight of LS are less than 6000.This injection thymosin content of peptides 7-10 mg/ml, determination of activity E-rosette meansigma methods 25-28%, yield 7-10 restrains thymosin/kilogram.

The method that the present invention makes castor otter medicine thymosin injection comprises following technical process:

1, extract thymus: the castor otter after slaughtering is extracted fresh thymus on one's body,

2, homogenate: fresh thymus is removed fat, is cut into fragment and places in the high-speed tissue mashing machine, add 2-4 by per kilogram thymus and rise normal saline, smash to pieces and made into homogenate in 3-7 minute, the centrifugal 2000-4000 of refrigerated centrifuger rev/min, 7-13 minute extracting solution,

3, heating foreigh protein removing: extracting solution is heated to 80-90 ℃ rapidly in water-bath, kept 12-18 minute, be cooled to rapidly below 10 ℃, the centrifugal precipitation of removing is collected clear liquid,

4, acetone precipitation: clear liquid is chilled to 4 ℃, add 4-6 times of volume-8--12 ℃ acetone placed 3-5 hour in-8--12 ℃, filtered collecting precipitation, vacuum drying gets acetone powder,

5, segmentation is saltoutd: acetone powder is dissolved in the PH6.8-7.2 phosphate buffer, make every milliliter to contain protein 23-28 milligram, the saturated ammonium sulfate solution that adds PH6.8-7.2 makes and reaches the 23-28% saturation, the centrifugal precipitation of removing, supernatant is transferred PH3.8-4.2, add ammonium sulfate and make saturation reach 48-52%, the centrifugal collection thing of saltouing

6, desalination, drying: the thing of saltouing is with the recrystallization desalination repeatedly of cold aqueous acetone, and after checking no sulfate, cold acetone is washed 1-3 time, and cold diethyl ether is washed 1-3 time, drain, and vacuum drying,

7, ultrafiltration: desalination dry powder is dissolved in normal saline and transfers the PH7.0-7.5 ultrafiltration, get ultrafiltrate,

8, preparation: in ampoule bottle, sealing by fusing gets required finished product with the ultrafiltrate sterile filling.

Manufacture method of the present invention is simple to operate, is suitable for producing in enormous quantities, adopts heating Deproteinization operation to make protein coagulation more complete, is easy to remove; Utilize the cold acetone that contains that the thing of saltouing is carried out the recrystallization desalination, shorten the time greatly, reduced opportunities for contamination.Thereby the castor otter medicine thymosin injection made from the inventive method is active high, avirulence, and no thermal source, clinical practice is safe and effective.

Be indefiniteness embodiment of the present invention below:

Castor otter after slaughtering is extracted fresh thymus on one's body, fresh thymus is removed fat, being cut into fragment places in the high-speed tissue mashing machine, add 3 liters of normal saline by per kilogram thymus, smash to pieces and made into homogenate in 5 minutes, centrifugal 3000 rev/mins of refrigerated centrifuger, got extracting solution in 10 minutes, extracting solution is heated to 85 ℃ rapidly in water-bath, kept 15 minutes, be cooled to rapidly below 10 ℃, the centrifugal precipitation of removing is collected clear liquid, and clear liquid is chilled to 4 ℃,-10 ℃ of acetone that add 5 times of volumes were placed 4 hours in-10 ℃, filter collecting precipitation, vacuum drying gets acetone powder, and acetone powder is dissolved in the PH7.0 phosphate buffer, make every milliliter to contain 25 milligrams in protein, the saturated ammonium sulfate solution that adds PH7.0 makes and reaches 25% saturation, the centrifugal precipitation of removing, and supernatant is transferred PH4.0, adding ammonium sulfate makes saturation reach 50%, the centrifugal collection thing of saltouing, the thing of saltouing is with the recrystallization desalination repeatedly of cold aqueous acetone, after checking no sulfate, cold acetone is washed 2 times, cold diethyl ether is washed 2 times, drains vacuum drying, desalination dry powder is dissolved in normal saline and transfers the PH7.2 ultrafiltration, get ultrafiltrate, in 2 milliliters of ampoule bottles, sealing by fusing gets required castor otter medicine thymosin injection finished product with the ultrafiltrate sterile filling.

The castor otter medicine thymosin injection that makes with the present invention test 25 patients clinicals, to intramuscular injection of different sick kind or subcutaneous injection 5-10 milliliters, and weekly twice, rheumatoid arthritis effective percentage 79%, myasthenia gravis are 75%.To chronic hepatitis B patient, the next day 1 injection, 10 milligrams of per injections continue existing two months, liver function recovery is normal, common sympton disappears, to the total effective rate of viral hepatitis more than 80%.

Claims (3)

1, a kind of castor otter medicine thymosin injection, it is characterized in that it being the cluster polypeptides matter that extracts from castor otter thymus, its thymosin dialysate is active high, its molecular weight is less than 10000, main component thymic hyperplasia element, thymosin, thymic humoral factor, serum thymic factor, the plain molecular weight of LS are less than 6000, and it is the medical thymosin injection that makes by the following method:

(1) extract thymus: the castor otter after slaughtering is extracted fresh thymus on one's body,

(2) homogenate: fresh thymus is removed fat, is cut into fragment and places in the high-speed tissue mashing machine, add 2-4 by per kilogram thymus and rise normal saline, smash to pieces and made into homogenate in 3-7 minute, the centrifugal 2000-4000 of refrigerated centrifuger rev/min, 7-13 minute extracting solution,

(3) heating foreigh protein removing: extracting solution is heated to 80-90 ℃ rapidly in water-bath, kept 12-18 minute, be cooled to rapidly below 10 ℃, the centrifugal precipitation of removing is collected clear liquid,

(4) acetone precipitation: clear liquid is chilled to 4 ℃, add 4-6 times of volume-8--12 ℃ acetone placed 3-5 hour in-8--12 ℃, filtered collecting precipitation, vacuum drying gets acetone powder,

(5) segmentation is saltoutd: acetone powder is dissolved in the PH6.8-7.2 phosphate buffer, make every milliliter to contain protein 23-28 milligram, the saturated ammonium sulfate solution that adds PH6.8-7.2 makes and reaches the 23-28% saturation, the centrifugal precipitation of removing, supernatant is transferred PH3.8-4.2, add ammonium sulfate and make saturation reach 48-52%, the centrifugal collection thing of saltouing

(6) desalination, drying: the thing of saltouing is with the recrystallization desalination repeatedly of cold aqueous acetone, and after checking no sulfate, cold acetone is washed 1-3 time, and cold diethyl ether is washed 1-3 time, drain, and vacuum drying,

(7) ultrafiltration: desalination dry powder is dissolved in normal saline and transfers the PH7.0-7.5 ultrafiltration, get ultrafiltrate,

(8) preparation: the ultrafiltrate sterile filling is got required finished product.

2, castor otter medicine thymosin injection according to claim 1 is characterized in that this injection thymosin content of peptides 7-10 mg/ml, determination of activity E-rosette meansigma methods 25-28%, and yield 7-10 restrains thymosin/kilogram.

3, a kind of method of making the described castor otter medicine of claim 1 thymosin injection comprises following technical process:

(1) get thymus: the castor otter after slaughtering is extracted fresh thymus on one's body,

(2) homogenate: fresh thymus is removed fat, is cut into fragment and places in the high-speed tissue mashing machine, add 2-4 by per kilogram thymus and rise normal saline, smash to pieces and made into homogenate in 3-7 minute, the centrifugal 2000-4000 of refrigerated centrifuger rev/min, 7-13 minute extracting solution,

(3) heating foreigh protein removing: extracting solution is heated to 80-90 ℃ rapidly in water-bath, kept 12-18 minute, be cooled to rapidly below 10 ℃, the centrifugal precipitation of removing is collected clear liquid,

(4) acetone precipitation: clear liquid is chilled to 4 ℃, add 4-6 times of volume-8--12 ℃ acetone placed 3-5 hour in-8--12 ℃, filtered collecting precipitation, vacuum drying gets acetone powder,

(5) segmentation is saltoutd: acetone powder is dissolved in the PH6.8-7.2 phosphate buffer, make every milliliter to contain protein 23-28 milligram, the saturated ammonium sulfate solution that adds PH6.8-7.2 makes and reaches the 23-28% saturation, the centrifugal precipitation of removing, supernatant is transferred PH3.8-4.2, add ammonium sulfate and make saturation reach 48-52%, the centrifugal collection thing of saltouing

(6) desalination, drying: the thing of saltouing is with the recrystallization desalination repeatedly of cold aqueous acetone, and after checking no sulfate, cold acetone is washed 1-3 time, and cold diethyl ether is washed 1-3 time, drain, and vacuum drying,

(7) ultrafiltration: desalination dry powder is dissolved in normal saline and transfers the PH7.0-7.5 ultrafiltration, get ultrafiltrate,

(8) preparation: in ampoule bottle, sealing by fusing gets required finished product with the ultrafiltrate sterile filling.