CN116948904A - Marine streptomycete and pigment production application thereof - Google Patents
Marine streptomycete and pigment production application thereof Download PDFInfo
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- CN116948904A CN116948904A CN202310920457.3A CN202310920457A CN116948904A CN 116948904 A CN116948904 A CN 116948904A CN 202310920457 A CN202310920457 A CN 202310920457A CN 116948904 A CN116948904 A CN 116948904A
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- 239000000049 pigment Substances 0.000 title claims abstract description 39
- 238000004519 manufacturing process Methods 0.000 title abstract description 7
- 241001655322 Streptomycetales Species 0.000 title abstract description 4
- 238000004043 dyeing Methods 0.000 claims abstract description 52
- 241001312734 Streptomyces parvulus Species 0.000 claims abstract description 21
- 241000187747 Streptomyces Species 0.000 claims abstract description 14
- 244000005700 microbiome Species 0.000 claims abstract description 10
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- 239000004744 fabric Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 244000005706 microflora Species 0.000 claims description 8
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- 238000004321 preservation Methods 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 241000933218 Streptomyces parvus Species 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
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- 239000001052 yellow pigment Substances 0.000 abstract description 8
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- 239000012752 auxiliary agent Substances 0.000 abstract description 5
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- 231100000252 nontoxic Toxicity 0.000 abstract description 3
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- 239000000243 solution Substances 0.000 description 18
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- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
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- 239000002585 base Substances 0.000 description 1
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- 235000012730 carminic acid Nutrition 0.000 description 1
- 239000004106 carminic acid Substances 0.000 description 1
- 210000000085 cashmere Anatomy 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 229940080423 cochineal Drugs 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
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- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 125000005842 heteroatom Chemical group 0.000 description 1
- 229940097275 indigo Drugs 0.000 description 1
- COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- 239000002649 leather substitute Substances 0.000 description 1
- IQPNAANSBPBGFQ-UHFFFAOYSA-N luteolin Chemical compound C=1C(O)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C(O)=C1 IQPNAANSBPBGFQ-UHFFFAOYSA-N 0.000 description 1
- LRDGATPGVJTWLJ-UHFFFAOYSA-N luteolin Natural products OC1=CC(O)=CC(C=2OC3=CC(O)=CC(O)=C3C(=O)C=2)=C1 LRDGATPGVJTWLJ-UHFFFAOYSA-N 0.000 description 1
- 235000009498 luteolin Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000000978 natural dye Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
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- 238000012216 screening Methods 0.000 description 1
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- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000000979 synthetic dye Substances 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention belongs to the field of microorganisms, and particularly relates to marine streptomycete and pigment production application thereof. The invention provides streptomyces minutissimus (Streptomyces parvulus) BG-Y2 which is deposited in the microorganism strain collection of Guangdong province at the date of 2023, 05 and 31, wherein the deposit number is as follows: GDMCC No:63511. the yellow pigment secreted by the streptomyces parvulus BG-Y2 is a natural pigment, has a good coloring effect on silk textiles as a dye, does not need to be extracted by a chemical reagent, has simple dyeing steps, does not need to add a dyeing auxiliary agent, is easy to color, has high color fastness, and is safe and nontoxic.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to marine streptomycete and pigment production application thereof.
Background
Dyeing and finishing is also called dyeing and finishing, and is a processing mode, and is also called pretreatment, dyeing, printing, finishing, washing water and the like. At present, dyeing of textile fabrics and ready-made clothes is mainly carried out by dye synthesized by chemistry in dyeing enterprises. These chemical dyes are derived from petrochemical materials, and most of the dye compounds have a benzene ring structure or contain a large number of conjugated double bonds. Meanwhile, hetero atoms such as O, N and the like are arranged in the molecule, and the components and the structure determine that a plurality of chemical synthetic dyes are difficult to be thoroughly degraded by organisms, so that printing and dyeing wastewater is difficult to treat, and certain dye molecules and heavy metals remain in terminal drainage.
The dyeing with biological dye is to dye fabrics with colored substances extracted from animals and plants. Methods for indigo dyeing using juice extracted from pine blue, luteolin, etc. have been available for thousands of years. Pigments extracted from cochineal worms are also widely used for garment dyeing. In recent years, microbial pigments have also begun to be applied to fabric dyeing. Compared with chemical dyeing, biological dyeing is more environment-friendly. The biological dye molecules can be thoroughly decomposed by organisms, and no toxic substances are discharged.
Microorganisms are the oldest species in nature and the most abundant species. There are many microorganisms that produce pigments of various colors. Microbial pigments have long been used in the food industry, such as monascus, melanin in soy sauce, chlorophyll, and the like. In recent years, microbial pigments have attracted attention in the fashion industry. Compared with plant pigment, microbial pigment can be obtained through fermentation, the production period is short, the cost is relatively low, and the method is more suitable for industrial production. Patent CN 112375791B discloses a method for preparing and dyeing streptomyces spectabilis haematochrome, which comprises the following steps: (1) culturing and fermenting; (2) ultrasonic extraction; (3) extraction; (4) purifying. Compared with other natural dyes, the Streptomyces spectabilis haematochrome has short production period and low cost, and is easy for industrial production; the fermentation medium is optimized, the phenomenon of pilling does not occur when the streptomyces spectabilis is cultivated, and the yield is higher; the dyeing liquid prepared from the Streptomyces spectabilis haematochrome is high in dye-uptake, and the soaping color fastness and the rubbing color fastness of the dyed silk fabric are both of grade 4 and accord with the dyeing standard of the silk fabric. The patent CN 110983816A discloses an application of actinomycin as a dye in printing and dyeing textile materials such as terylene, chinlon, cotton, silk and the like by using actinomycin obtained from marine plants as a natural pigment, and has good dyeing effect and good antibacterial effect; especially has good dyeing effect on silk fabrics, the dry rubbing fastness is 4-5 grade, the wet rubbing fastness is 4 grade, the soaping fastness is 4 grade, actinomycin is applied to dyed materials, the raw materials are easy to obtain, the cost is low, and the actinomycin has good application prospect in industry as a functional dye due to antibacterial activity. However, the extraction of the pigment requires an organic solvent and a dyeing auxiliary agent in the dyeing process, and the actual green and safe performance is not realized. Although there are many attempts, in general, microbial pigment dyeing is still in the stage of pigment-producing strain separation and laboratory dyeing pilot-scale, the coloring is difficult and the color fastness is lower, the screening separation dyeing process is more simplified, the pigment with good coloring capability is not extracted by using chemical solvents and dyeing auxiliary agents, and the requirement of industrial printing and dyeing is met by improving the coloring process.
Disclosure of Invention
In order to solve the problems, the invention provides streptomyces minutissimus (Streptomyces parvulus) BG-Y2, which is characterized in that the streptomyces minutissimus is deposited in the microorganism strain collection of Guangdong province at the date of 2023, 05 and 31, and the deposit number is: GDMCC No:63511. the yellow pigment secreted by the streptomyces parvulus BG-Y2 is a natural pigment, has a good coloring effect on silk textiles as a dye, does not need to be extracted by a chemical reagent, has simple dyeing steps, does not need to add a dyeing auxiliary agent, is easy to color, has high color fastness, and is safe and nontoxic.
In one aspect, the invention provides Streptomyces parvulus (Streptomyces parvulus) BG-Y2 deposited at the microorganism strain collection in Guangdong province at month 31 of 2023 under the accession number: GDMCC No:63511.
on the other hand, the invention provides a fermentation method of the streptomyces microflora BG-Y2, which comprises the step of inoculating the streptomyces microflora BG-Y2 into a culture solution of first Gao's disease.
Specifically, the culture solution of Gao's No. oneThe formulation of (C) can be soluble starch 15-25g/L and KNO 3 0.5-1.5g/L，K 2 HPO 4 0.2-0.8g/L，MgSO 4 ·7H 2 O 0.2-0.8g/L，NaCl 25-35g/L，FeSO 4 ·7H 2 O 0.01-0.02g/L。
Preferably, the formula of the culture solution of Gao's No. one is soluble starch 20g/L and KNO 3 1g/L，K 2 HPO 4 0.5g/L，MgSO 4 ·7H 2 O 0.5g/L，NaCl 30.5g/L，FeSO 4 ·7H 2 O 0.01g/L。
On the other hand, the invention also provides a preparation method of the pigment solution, which comprises the following steps:
(1) Inoculating the streptomyces microflora BG-Y2 into the culture solution of the first Gao's strain for culture;
(2) Centrifuging and filtering to obtain supernatant.
Specifically, the culture temperature in step (1) may be 25 to 30℃and the culture time may be 5 to 10 days.
Preferably, the incubation temperature in step (1) is 28℃and the incubation time is 7d.
Specifically, the pigment solution is a yellow pigment solution.
In yet another aspect, the present invention provides a pigment solution prepared by the aforementioned preparation method.
In yet another aspect, the present invention provides a method of dyeing a fabric comprising immersing the fabric in the pigment solution described above.
Specifically, the pigment solution is a yellow pigment solution.
Specifically, the fabric is silk fabric, and the bath ratio can be 1:30-40.
Preferably, the bath ratio is 1:35.
Specifically, the fabric dyeing method also comprises the steps of heat preservation dyeing after temperature rising, cleaning and drying.
Further specifically, the post-warming thermal-insulation dyeing comprises two warming steps.
Preferably, the temperature is raised to 55-65 ℃ for the first time, and the temperature is kept for dyeing for 25-35min; heating to 75-85 ℃ for the first time, and preserving heat and dyeing for 15-25min.
Further preferably, the temperature is raised to 60 ℃ for the first time, and the dyeing is carried out for 30 minutes; and heating to 80 ℃ for the second time, and preserving heat and dyeing for 20min.
In still another aspect, the invention provides application of the streptomyces minutissimus BG-Y2 or pigment solution in textile and leather printing industry.
Specifically, the spinning includes, but is not limited to: cashmere, wool, silk, cotton, chemical fiber or modal;
the leather includes, but is not limited to: cow leather, sheep leather, PVC leather, vegetable fiber synthetic leather or mycelium leather.
In yet another aspect, the invention provides the use of the aforementioned Streptomyces parvulus BG-Y2 or pigment solutions in cosmetics.
Specifically, the cosmetic includes, but is not limited to: hair dye, lipstick or foundation.
In still another aspect, the invention provides the use of the aforementioned Streptomyces parvulus BG-Y2 or pigment solutions for preparing pigments or paints.
Specifically, the coating includes, but is not limited to: liquid, powder or high solids coatings.
The invention has the technical effects that:
(1) Streptomyces parvus BG-Y2 has strong reproductive capacity, short growth cycle and more yellow pigment secretion;
(2) The pigment is not extracted by chemical reagent, the dyeing step is simple, and the dyeing auxiliary agent is not added;
(3) The color fastness is high;
(4) Is safe and nontoxic.
Preservation description:
strain name: streptomyces parvus (Streptomyces parvulus) BG-Y2;
preservation number: GDMCC No:63511;
taxonomic name: streptomyces parvulus;
preservation time: 2023, 05, 31;
preservation unit: the collection of microorganism strains in Guangdong province;
preservation address: guangzhou city first middle road No. 100 college No. 59 building 5.
Drawings
FIG. 1 is a photograph of a colony of Streptomyces parvulus (Streptomyces parvulus) BG-Y2 cultured for 7d.
FIG. 2 is a photograph of fermentation broth of Streptomyces parvulus (Streptomyces parvulus) BG-Y2 cultured for 7d.
FIG. 3 is a photograph of yellow pigment-stained silk secreted by Streptomyces parvulus (Streptomyces parvulus) BG-Y2.
Detailed Description
The present invention will be described in further detail with reference to the following examples, which are not intended to limit the present invention, but are merely illustrative of the present invention. The experimental methods used in the following examples are not specifically described, but the experimental methods in which specific conditions are not specified in the examples are generally carried out under conventional conditions, and the materials, reagents, etc. used in the following examples are commercially available unless otherwise specified.
Example 1 isolation and identification of strains
Soil sample: guangxi seaside mangrove soil.
Isolation medium: the formula of the culture medium of Gaoshi No.1 is as follows: soluble starch (20 g/L), KNO 3 (1g/L)，K 2 HPO 4 (0.5g/L)，MgSO 4 ·7H 2 O(0.5g/L)，NaCl(0.5g/L)，FeSO 4 ·7H 2 O (0.01 g/L), agar (20 g/L). The culture medium was formulated with sterile filtered seawater, ph=7.4-7.6. Sterilizing with steam at 121deg.C for 20min.
Fermentation medium: the formula of the modified Gaoshi first culture solution added with 3% NaCl is as follows: soluble starch (20 g/L), KNO 3 (1g/L)，K 2 HPO 4 (0.5g/L)，MgSO 4 ·7H 2 O(0.5g/L)，NaCl(30.5g/L)，FeSO 4 ·7H 2 O (0.01 g/L), deionized water was added to a volume of 1L. ph=7.4-7.6. Sterilizing with steam at 121deg.C for 20min.
1.1 isolation of strains
Spreading the collected soil sample in sterile culture dish, and naturally drying at 25-28deg.CGrinding for later use after 7-14 d. Firstly, placing a soil sample into a 70 ℃ oven for 1 hour to kill bacteria, then weighing 5g of the soil sample, placing the soil sample into a 150mL triangular flask containing 15-20 small glass beads which are sterilized, adding 45mL of sterile water, and shaking the triangular flask at 30 ℃ for 20 minutes; followed by gradient dilution with sterile water. The dilution gradient required for the experiment was 10 -2 、10 -3 、10 -4 And 10 -5 A total of 4. 100 mu L of soil dilutions with different dilution gradients are respectively and uniformly coated in the separation culture medium by a coating rod, and are cultured for 10-14d at 28 ℃ to check colony pigment production. A single colony which obviously secretes yellow pigment is selected, further separated and purified and then stored in 20% glycerol at a low temperature.
1.2 morphological observations
The purified strain is inoculated in the separation culture medium, and hypha grows well. The aerial hyphae are orange, the surfaces of the fungus fur are raised, and the spore filaments are grey. After 7d incubation at 28℃yellow secreted pigments were visible in the medium, the hyphae in the medium being orange yellow.
1.3 identification of species
The isolated strain was sent to the engineering bioengineering (Shanghai) Co., ltd for identification. Bacterial 16S rRNA universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACGGCTACCTTGTTACGACTT-3') were used. After PCR, 3 segments are subjected to series analysis, 16s rRNA full-length sequences are spliced, and then comparison analysis is performed on NCBI databases, so that the sequence similarity with streptomyces minutissimus (Streptomyces parvulus) in the databases is highest. The 16S rRNA base is shown as SEQ ID NO.1 and named as Streptomyces parvus (Streptomyces parvulus) BG-Y2, which is deposited in the microorganism strain collection of Guangdong province at the month 31 of 2023 with the deposit number: GDMCC No:63511.
example 2
2.1 Streptomyces microflora (Streptomyces parvulus) BG-Y2 yellow secretory pigments for silk dyeing, comprising the following steps:
(1) Fermentation culture and pigment preparation
Inoculating the purified strain into a solid separation culture medium, and culturing for 10 days at 28 ℃; spores were inoculated into 100mL fermentation medium. At a constant temperature of 28 ℃, the culture is carried out by shaking at a shaking table rotation speed of 160rpm for 7 days. After the fermentation broth was centrifuged (8000 g,10 min), the supernatant was sterile filtered, and the filtrate was a pigment solution.
(2) Dyeing of fabric
The pigment solution was used for dyeing silk. 40X 40cm silk is treated according to a bath ratio of 1:35 is soaked in the pigment solution, the temperature is raised to be 3 ℃ per minute, the temperature is raised to be 60 ℃ and then the dyeing is carried out for 30min, the temperature is raised to be 80 ℃ and the dyeing is continued for 20min. Taking out, cleaning with water, and oven drying at 60deg.C.
2.2 color fastness detection
And (3) feeding the dyed silk to a middle spinning standard (Shenzhen) detection limited company for detecting the washing fastness, the acid perspiration fastness, the alkali perspiration fastness, the friction fastness, the washing fastness, the light fastness and the non-chlorine bleaching fastness. The detection results are shown in Table 1.
TABLE 1
The results show that the yellow pigment produced by the streptomyces minutissimus (Streptomyces parvulus) BG-Y2 has good coloring effect on silk. The color fastness to water reaches 3-4 levels, the color fastness to rubbing reaches 4-5 levels, the color fastness to non-chlorine bleaching reaches 3 levels, the color fastness to light reaches 3-4 levels, and the requirements of silk clothing are met; the washing fastness is grade 2, and natural auxiliaries can be adopted subsequently to improve the washing fastness.
Claims (10)
1. Streptomyces parvus (Streptomyces parvulus) BG-Y2 deposited at the microorganism strain collection in Guangdong province at month 31 of 2023 under the accession number: GDMCC No:63511.
2. the fermentation method of the streptomyces microflora BG-Y2 of claim 1, comprising inoculating the streptomyces microflora BG-Y2 to a culture broth of No. one gao; the formula of the Gaoshi first culture solution is as follows: soluble starch 15-25g/L KNO 3 0.5-1.5g/L，K 2 HPO 4 0.2-0.8g/L，MgSO 4 ·7H 2 O0.2-0.8g/L，NaCl 25-35g/L，FeSO 4 ·7H 2 O 0.01-0.02g/L。
3. A method for preparing a pigment solution, comprising the steps of:
(1) Inoculating the streptomyces microflora BG-Y2 of claim 1 into the culture solution of claim 2 for culturing;
(2) Centrifuging and filtering to obtain supernatant.
4. A pigment solution prepared by the method of claim 3.
5. A method of dyeing a fabric comprising immersing the fabric in the pigment solution of claim 4.
6. The method for dyeing fabrics according to claim 5, wherein the fabrics are silk fabrics and the bath ratio is 1:30-40.
7. The method for dyeing fabrics according to claim 5, further comprising the steps of heating, dyeing with heat, washing and drying.
8. The method of dyeing fabrics according to claim 7, wherein the post-warming, thermal-insulation dyeing comprises two warming steps.
9. The method for dyeing fabrics according to claim 8, wherein the temperature is raised to 55-65 ℃ for the first time, and the fabric is dyed for 25-35min with heat preservation; heating to 75-85 ℃ for the second time, and keeping the temperature for dyeing for 15-25min.
10. Use of the streptomyces microflora BG-Y2 of claim 1 or the pigment solution of claim 4 in textile, leather printing and dyeing.
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