CN116926104A - Transfer method of nuclear male sterile maintainer line - Google Patents

Abstract

The invention relates to a method for transferring a nuclear male sterile maintainer line. The invention designs a gene silencing element aiming at a male gamete abortive element/lethal element in a nuclear male sterile maintainer line, which can inhibit part or all functions of the male gamete abortive element/lethal element in the nuclear male sterile maintainer line, so that transgenic pollen containing a nuclear male sterile maintainer line carrier element which is active and viable can be produced, the nuclear male sterile maintainer line can be used as a male parent to transform to other materials, and the purpose of replacing cytoplasm of the nuclear male sterile maintainer line is realized.

Description

Transfer method of nuclear male sterile maintainer line

Technical Field

The invention relates to the technical field of agricultural biology, in particular to a transformation method for a nuclear male sterile maintainer line, a plant expression vector thereof, a construction method and application thereof.

Background

Hybrid vigour is a phenomenon in which hybrid offspring surpass parents in one or more traits, and is ubiquitous in the biological kingdom. Crop heterosis utilization is an important means for agricultural yield increase, and the core of the heterosis utilization industrialization is male sterility or male sterile line of female parent. The nuclear-cytoplasmic interactive sterile line and the photo-thermo-sensitive sterile line are applied to crop breeding, so that cross breeding of crops is promoted to a large extent, but the nuclear-cytoplasmic interactive sterile line and the photo-thermo-sensitive sterile line have obvious defects, such as insufficient assembly, unstable fertility and the like, and the breeding potential of the cross crops cannot be fully exerted.

The male fertility of recessive nuclear male sterility is controlled by only one pair of recessive nuclear genes and is not influenced by light temperature environment. And because the gene is a very rare recessive mutation, the loci of the genome of most varieties are dominant genes of wild type, so almost any variety can be theoretically used as a recovery line of recessive nuclear male sterility to be hybridized with the dominant genes to generate a fertile offspring (hybrid). However, it has long been limited by the inability to maintain male sterility of their progeny on a large scale, and therefore, it has not been industrially applicable.

The basic principle of the present application of the nuclear male sterile line is that fertility restoring gene element, male gamete aborting element/lethal element and seed color selection element are integrated into one carrier and transferred into the nuclear male sterile line to create a maintainer line of the nuclear male sterile line, and the transgenic pollen of the nuclear male sterile maintainer line is aborted or killed due to the special function of the male gamete aborting element/lethal element, so that the transgenic pollen cannot be transferred out. Therefore, the pollen of other varieties can be accepted only as female parent in the process of transferring the element or the carrier of the nuclear male sterile maintainer line, but the element or the carrier of the nuclear male sterile maintainer line can not be pollinated as male parent, so that the transfer freedom of the nuclear male sterile maintainer line is limited, and the cytoplasm of the nuclear male sterile maintainer line and the cytoplasm of the sterile line are also unified. It is therefore necessary to find a method which enables the transformation of a nuclear male sterile maintainer line as a male parent.

Disclosure of Invention

In the prior art, the basic principle of the utilization of the nuclear male sterile line is to integrate fertility restoration gene elements, male gamete abortive elements/lethal elements and seed color sorting elements into one genetic transformation vector, transfer the genetic transformation vector into the nuclear male sterile line and create a maintainer line of the nuclear male sterile line.

The nuclear male sterile maintainer line contains two types of pollen, one of which is dead and can not mature due to the fact that the pollen contains the genetic transformation vector, namely the male gamete abortive element/lethal element; the other does not contain genetic transformation vector, is non-transgenic pollen and can grow normally. Thus, only one mature pollen is ultimately formed, i.e., non-transgenic pollen without the genetic transformation vector. Therefore, the pollen of other varieties can be accepted only as female parent in the process of transferring the element or the carrier of the nuclear male sterile maintainer line, but the element or the carrier of the nuclear male sterile maintainer line can not be pollinated as male parent, so that the transfer freedom of the nuclear male sterile maintainer line is limited. Due to the characteristics, the nuclear male sterile maintainer line and the cytoplasm of the sterile line thereof have the problem of singulation, and have potential plant diseases and insect pests risks.

If the maintainer line only hybridizes as a female parent with other breeding materials, the bred maintainer lines all have the same cytoplasmic (mitochondrial and chloroplast) genome, which results in a cytoplasmic genetic singulation scenario in the maintainer line. However, cytoplasmic genetic diversity has a significant impact on the traits of plants. The cytoplasmatic effect widely influences the properties of crop yield, plant height, leaf width, protein content, flowering phase, seed oil content and the like. The cytoplasm unification of the hybrid crops not only has the risk of inducing disease outbreaks, but also limits the long-term breeding effect of crop varieties.

The invention aims to provide a transfer system and a transfer method of a nuclear sterile maintainer line, which realize the purpose of replacing cytoplasm of the nuclear male sterile maintainer line.

In a first aspect, the present invention provides a transfer system (hereinafter abbreviated as GATi system) of a nuclear male sterility maintainer line, the transfer system comprising: a gene silencing element of male gamete abortive element/lethal element, a silencing tag reporting element and a selectable tag element; the gene silencing element is used to inhibit or partially inhibit the function of the male gamete abortive element/lethal element.

The invention provides a transfer system, which aims at male gamete abortive elements/lethal elements or male gamete abortive elements/lethal sequences in different nuclear male sterile maintainer lines, designs gene silencing elements thereof for inhibiting functions of the male gamete abortive elements/lethal genes, and ensures that pollen containing the male gamete abortive elements/lethal genes is incompletely aborted or dead, so that the nuclear male sterile maintainer line can generate pollen carrying transgenes (nuclear male sterile maintainer line elements or vectors), and is used as a male parent to pollinate to other materials, thereby realizing the purpose of replacing cytoplasm of the nuclear male sterile maintainer line and sterile line. A silencing tag reporter linked to a silencing element of a pollen-specific abortive element in the GATi system as a reporter gene for the silencing element for displaying and sorting seeds or plants containing the silencing element; screening marker elements for genetic transformation screening and differentiation of GATi seeds or plants.

In the transgenic system provided by the invention, a method for inhibiting or partially inhibiting the function of male gamete abortive/lethal elements using a gene silencing element comprises:

1) Inhibiting or reducing transcription of male gamete abortive/lethal elements, including using RNAi, amiRNA, siRNA, asRNA or Crispri, promoter competitive binding;

2) Inhibiting or reducing protein translated from male gamete abortive/lethal elements;

3) The biochemical function of the product is inhibited by binding, chemical modification, or specific degradation to the product of the male-sterile element/lethal element expression cassette.

Preferably, the gene silencing technique includes RNAi, amiRNA, siRNA, asRNA, crispri and the like, which inhibit the function of male gamete abortive elements/lethal elements in the nuclear male sterile maintainer line; more preferably, the gene silencing technique is RNA interference technique called RNAi or AmiRNA for short; the gene silencing element consists of a promoter-stem loop structure-terminator, or a promoter-antisense coding sequence-terminator, or a target binding sequence of a promoter-dCas 9-terminator, male gametocarrhea element/lethal element. The pollen-specific abortive gene or pollen-specific lethal gene is OsAA, hvAA, zmAA, orfH79 and Dam gene, protease gene, oxidase gene or rnase gene, etc., or promoter elements such as pG47, PC32, PC10, PC40, zm13, etc. in expression cassettes containing these genes. In the transformation system provided by the invention, if the male gamete abortive element/lethal element is a rice alpha-amylase (OsAA) gene expression cassette, the gene silencing element is a hairpin structure expression cassette, and the hairpin structure expression cassette contains a hairpin structure formed by DNA fragments shown in SEQ ID No. 1-3.

The silencing tag reporter element is a different color-selective marker gene from the nuclear male sterile maintainer line to facilitate differentiation of different functional seeds, and may be GUS, GFP, YFP, BFP, CFP, RFP or other colors or seed shapes as a marker of gene silencing material.

The screening marker element is used for genetic transformation screening of GATi and distinguishing seeds or plants with different purposes. Can be an antibiotic marker gene (antibiotic markers such as hygromycin, gentamicin and kanamycin gene which can be screened) expression cassette, a herbicide screening marker gene (such as glyphosate resistance gene, bispyribac-sodium resistance gene, glufosinate resistance gene and the like) expression cassette or a chemical reagent resistance marker gene (such as herbicide resistance gene and the like) expression cassette.

Preferably, the reporter gene element is a GFP or GUS gene expression cassette and the selectable marker gene element is a hygromycin gene expression cassette.

The present invention provides a transfer system for nuclear male sterility retention systems utilizing male gamete abortive/lethal elements, including but not limited to GAT technology (for specific information on GAT technology, see chinese patent application nos. 202010378962.6, 202010379287.9, 202010378963.0, 2021113336788).

The following description uses the GAT nuclear male sterile maintainer line as a substitute, but the invention is not only applicable to the nuclear male sterile maintainer line represented by GAT, but also applicable to other types of nuclear male sterile lines, and the transformation system provided by the invention, wherein the vectors, plants or seed materials containing the gene silencing element, the silencing mark reporting element and the screening mark element of the male gamete abortive element/lethal element are simply referred to as GATi vectors, GATi plants or GATi seed materials.

In a second aspect, the present invention provides a method of transferring a nuclear male sterile maintainer line, comprising:

(1) Transferring the transfer system into a material A to obtain a first plant; the first plant is also called the GATi plant;

(2) Hybridizing the first plant obtained in the step (1) serving as a male parent and the nuclear male sterile maintainer line material B serving as a female parent to obtain a second plant; the second plant comprises F1 generation and F1 generation selfing or filial generation materials still containing nuclear male sterile maintainer line sites and transfer system sites;

(3) And pollination the second plant as a male parent into other plant materials to obtain a nuclear male sterile maintainer line which is different from the nuclear male sterile maintainer line material B cytoplasm.

In the method of the present invention, the first plant comprises a gene silencing element for inhibiting or partially inhibiting the function of a male gamete abortive element/lethal element.

In the method of the invention, the silencing-marker reporter element is different from the color-selection marker gene in the nuclear male sterile maintainer line, and is used for distinguishing seeds with different functions. In the offspring of the obtained hybrid, the use is distinguished by different types of markers.

Specifically, the second plant generation is used as a male parent to pollinate other plant materials, and the existing silencing element marker (GATi) and nuclear male sterile maintainer line marker can be used for subsequent continuous transfer;

in the offspring, only the nuclear male sterile maintainer line is marked and can be used for variety breeding or seed production of the nuclear male sterile line or the maintainer line;

in their offspring, only the silencing element tag (GATi) is available for crossing with the corresponding nuclear male sterile maintainer line.

The transformation method provided by the invention increases the transformation freedom degree of the nuclear male sterile maintainer line, and can transfer the nuclear male sterile maintainer line element into the material of new cytoplasmic germplasm resources to realize the replacement of cytoplasm.

In a third aspect, the invention provides a method for improving cytoplasmic genetic diversity of a nuclear male sterile maintainer line, wherein a first plant is used as a male parent to hybridize with an initial nuclear male sterile maintainer line material to obtain a second plant, and seeds of the second plant are subjected to selfing, backcrossing or hybridization with other materials to obtain a target plant;

the first plant carries a transfer system site i;

the transformation system site i comprises: a gene silencing expression cassette for inhibiting male gamete abortive elements/lethal elements in the nuclear male sterility maintainer line site G, a marker reporting element expression cassette for the transformation system site i, and a selectable marker element expression cassette for the transformation system site i.

The nuclear male sterile maintainer line locus G includes, but is not limited to, a male sterile restorer element expression cassette, a male gamete abortive element/lethal element expression cassette, and a maintainer selectable marker expression cassette.

Specifically, in the method provided by the invention, the nuclear male sterility refers to the constitutive male sterility caused by nuclear genes, and the sterile phenotype is caused by 1 or 2-3 gene mutations (recorded as ms).

In the methods provided herein, the genotype of the first plant is homozygous ii or heterozygous i-; preferably, the genotype of the first plant is ii;

the second plant genotype is homozygous GG/ii or heterozygous G-/i-or semi-heterozygous GG/i-, G-/ii, which may carry a nuclear male sterility gene ms, preferably the genotype of the second plant is msms/GG/ii or msms/G-/ii.

In the method provided by the invention, the target plant is a new nuclear male sterile maintainer line obtained by breeding, and the cytoplasm of the target plant is different from that of the initial nuclear male sterile maintainer line; preferably, the second plant is taken as a male parent, the female parent selects a nuclear male sterile maintainer line with different cytoplasm from the initial nuclear male sterile maintainer line, the sterile line or other materials are hybridized, the intermediate materials are obtained through selfing back cross, and finally a new nuclear male sterile maintainer line is obtained, namely the target plant.

In a fourth aspect, the invention also provides a plant expression vector GATi. The vector is used as an application condition for specifically describing functions and methods of the first three aspects by way of example, the vector contains a hairpin structure formed by DNA fragments shown by SEQ ID No.1-3 or a nucleotide sequence of the hairpin structure is shown by SEQ ID No.4-6, and the plant expression vector is used for inhibiting functions of male gamete abortive elements/lethal elements in the nuclear male sterile maintainer line site G.

The GATi plant expression vector provided by the invention contains a hairpin structure expression cassette, wherein the hairpin structure expression cassette contains a hairpin structure formed by DNA fragments shown in SEQ ID No. 1-3.

Wherein the DNA fragment shown in SEQ ID No.1 is a forward DNA fragment with the length of 310bp based on the coding frame of the rice alpha-amylase gene, and the DNA fragment shown in SEQ ID No.3 is a DNA fragment with the length of 310bp based on the coding frame of the rice alpha-amylase gene, which is reversely complementary with the DNA fragment shown in SEQ ID No. 1. SEQ ID No.2 is an intron sequence (Rice intron) from the Rice finger gene.

The DNA fragments shown in SEQ ID Nos. 1 to 3 are arranged in order from the upstream to the downstream. The hairpin expression cassette can be transcribed in transformed plant cells to form a hairpin-type secondary structure, the DNA fragment shown in SEQ ID No.2 forms a hairpin "loop", and the DNA fragments shown in SEQ ID No.1 and 3 complement each other to form a hairpin "stem".

Wherein, the hairpin structure expression cassette further comprises a plant constitutive promoter or a plant tissue specific promoter positioned at the upstream of the hairpin structure, and a terminator positioned at the downstream of the hairpin structure.

The GATi vector provided by the invention contains a selectable marker expression cassette, wherein the selectable marker expression cassette contains a promoter, a marker gene and a terminator, and the promoter is a Ubi promoter, a CAMV35S promoter or an action promoter of rice or corn; the marker gene is a gene of an enzyme capable of generating color change, a fluorescent marker gene, an antibiotic marker gene, a herbicide screening marker gene or an anti-chemical reagent marker gene, and the terminator is a NOS terminator or a Ubi terminator.

Preferably, the promoter is ZmUbiP, osUbiP, CAMV SP, actin1P, PC32, PC10, LTP2, gt1, osPL16P or OsPL10P; the terminator is NOST, osUbiT or AtHSPT.

The GATi vector provided by the invention contains a color marker gene GUS or GFP, and the color marker gene is different from a color selection marker gene of a nuclear male sterile maintainer line material by a GUS staining method or fluorescence marking of the GATi material so as to be convenient for distinguishing. The offspring material has the silencing element mark and the nuclear male sterile maintainer line mark, and can be used for subsequent continuous transfer; only the nuclear male sterile maintainer line is marked and can be used for variety breeding or seed production of the nuclear male sterile line or the maintainer line; only silencing element markers are available for hybridization with the corresponding nuclear male sterile maintainer line.

The GUS dyeing method is disclosed in my issued patent, application number: 201610701191.3.

the GFP viewing method comprises the following steps: the transgenic materials of GATi-Osaai-1gfp, GATi-Osaai-2gfp and GATi-Osaai-3gfp were excited under blue light fluorescent lamp, and the positive seeds containing 3 GATi vector transgenes were seen as green fluorescence.

After the GAT maintainer line material and the GATi material are hybridized, the color selection marker element of the exemplified GAT maintainer line material is red fluorescent protein, so that the hybrid is selfed or pollinated again to the new GAT maintainer line material which only contains the red fluorescent protein and is transferred in the offspring generated by other materials; GATi material containing only GUS-dyeable or GFP-fluorescent; the material containing GATi and nuclear male sterile maintainer line can be used for selfing propagation or used as male parent to realize nuclear male sterile maintainer line hybridization transformation.

Since the GAT maintainer line is antibiotic or herbicide resistant, e.g., the screening resistance of the GAT maintainer line material is ALS-like herbicide resistant, GATi is hygromycin resistant. Therefore, if the filial generation generated by the selfing or the re-pollination of the hybrid seeds to other materials only contains the corresponding antibiotic or herbicide resistance of the nuclear male sterile maintainer line material, the material is a new nuclear male sterile maintainer line material; the offspring only contains hygromycin resistance is GATi material; both of the offspring are materials containing GATi and the nuclear male sterile maintainer line, and can be continuously used for selfing propagation or used as a male parent to realize the hybrid transformation of the nuclear male sterile maintainer line.

In addition to phenotypic identification screening, the corresponding nuclear male sterile maintainer line and GATi can be used for screening and distinguishing. If the offspring generated by the selfing or the re-pollination of the hybrid seeds is detected by genome PCR, the offspring only contains the nuclear male sterile maintainer line molecular marker and is a new nuclear male sterile maintainer line material; the offspring only contains GATi molecular markers which are GATi materials; both of the offspring are materials containing GATi and the nuclear male sterile maintainer line, and can be continuously used for selfing propagation or used as a male parent to realize the hybrid transformation of the nuclear male sterile maintainer line.

The use of the above-described transformation methods for the preparation of plant nuclear male sterile maintainer lines is also claimed, as will be appreciated by those skilled in the art.

In the application, the plant is hybrid crops such as rice, corn, cotton, rape or wheat;

preferably, the plant is rice or maize.

In one embodiment of the invention, a method of obtaining a GATi material comprises:

(1) Constructing a plant expression vector: the DNA fragment shown in SEQ ID No.1-3 is connected between SacI and BamHI sites of a plant binary transformation vector PTCK303 by an overlapping method to obtain a plant expression vector GATi-Osaai-1;

(2) Conversion: transforming plant cells or tissues by using a conventional biological method such as an agrobacterium-mediated genetic transformation method, a gene gun method, a pollen tube channel method and the like to obtain a GATi transgenic plant.

In a similar manner, GATi transgenic plants were also obtained with GATi-Osaai-2 and GATi-Osaai-3 vectors.

Wherein, the plant expression vectors GATi-Osaai-1, GATi-Osaai-2 and GATi-Osaai-3 respectively have nucleotide sequences shown in SEQ ID No.4, SEQ ID No.5 and SEQ ID No. 6.

Meanwhile, the GUS expression cassette in the pTCK303 vector can be replaced by a fluorescent protein expression cassette, preferably a GFP expression cassette is used, and the obtained GATi vectors are named GATi-Osaai-1GFP, GATi-Osaai-2GFP and GATi-Osaai-3GFP, and the sequences of the GATi-Osaai-3GFP are shown as SEQ ID No.18, SEQ ID No.19 and SEQ ID No.20, so that corresponding transgenic materials are obtained.

The invention also provides an application method of the GATi plant expression vector in the nuclear male sterile maintainer line material transfer process.

The method of the invention is to use GATi material to hybridize with nuclear male sterile maintainer line material, and the obtained F1 seed can lead the transgenic pollen containing nuclear male sterile maintainer line element not to be completely degraded or killed in the flowering phase, thereby having partial or complete activity and being pollinated to other materials.

The invention has the beneficial effects that:

(1) The GATi system provided by the invention can be used for transferring a plurality of plants, in particular to nuclear male sterile maintainer lines of crops; the cytoplasmic singulation problem of the sterile maintainer line and the sterile line is solved, and the pollen containing the GTA vector is used as a male parent for pollination, so that the cytoplasm is replaced, and the occurrence of susceptibility or other harm is avoided; meanwhile, the problem of the sterility of the sterile maintenance line is solved, so that the sterile maintenance line can be used as a female parent or a male parent for hybridization and transformation (when a GATi system is not available, the sterile maintenance line can only be used as the female parent for transformation).

(2) The phenotype in the filial generation or the selfing generation is accurately screened out through the synergistic effect of various elements, and the generation only contains the corresponding antibiotics or herbicide resistance of the nuclear male sterile maintainer line site or specific seed fluorescence such as red fluorescent seeds, so that the novel nuclear male sterile maintainer line material is obtained; the offspring may contain only hygromycin resistant or specific seed fluorescence, such as green seed fluorescence, or may be labeled with a specific staining label, such as GUS, as GATi material; both of the offspring are materials containing GATi and nuclear male sterile maintainer line sites, and can be continuously used for selfing propagation or used as male parent to realize nuclear male sterile maintainer line hybridization transformation.

(3) The GATi and nuclear male sterile maintainer line locus prepared by the invention can ensure that part or all of transgenic pollen expressing male gamete abortive elements/lethal elements is normal, and can be used as male parent to realize free transformation of the nuclear male sterile maintainer line.

(4) The transformation method provided by the invention can be used for transforming a plurality of plants, in particular to nuclear male sterile maintainer lines of crops; the method provided by the invention has the advantages that pollen containing a nuclear male sterile maintainer line carrier is used as a male parent for pollination, the cytoplasm is replaced, and the risk of susceptibility or other harm is reduced; meanwhile, the problem of the sterility of the sterile maintenance line is solved, so that the sterile maintenance line can be used as a female parent or a male parent for the hybridization and the transformation of the sterile maintenance line carrier (the sterile maintenance line can only be used as the female parent for the transformation when no GATi system exists).

(5) The method for improving the cytoplasmic genetic diversity of the nuclear male sterile maintainer line provided by the invention realizes the replacement of the cytoplasmic genetic material of the maintainer line through traditional hybridization for the first time. The invention maintains the cytoplasmatic genetic diversity of the maintainer line breeding material of the hybrid crops, avoids the damage caused by cytoplasmatic singulation to long-term breeding and production, and has important significance to long-term breeding of the hybrid crops.

Drawings

FIG. 1 is a diagram showing the digestion of the backbone vector PTCK303 of the present invention. Wherein M is Marker, CK is PTCK303 vector plasmid which is not subjected to enzyme digestion, 1-4 are PTCK303 skeleton vector which is subjected to enzyme digestion by SacI and BamHI, and a fragment of about 500bp can be cut.

FIG. 2 is a diagram showing EcoRI cleavage of the recombinant plasmid GATi-Osaa-1 of the present invention. Wherein M is Marker, CK is PTCK303 skeleton carrier cut by enzyme, about 1.5kb fragment can be cut out; 1-7 is an digested GATi-Osaa-1 recombinant plasmid, and an about 2kb fragment can be excised.

FIG. 3 is a PCR detection electrophoretogram of transgenic sample plants of the GATi-Osaa-1 vector of the invention. Wherein M is Marker, -is blank control, CK-is ZH11 non-transgenic plant genome DNA, and 1-22 are transgenic plant genome DNA obtained by screening.

FIG. 4 is a graph showing the gene expression level of the transgenic T1 strain Osaa of GATi-Osaa-1, GATi-Osaa-2 and GATi-Osaa-3 of the present invention. Wherein WT is a wild-type control strain; CK1+ and CK2+ are two transgenic line positive controls of the Osaa overexpression vector; l145 is the abbreviated number of GATi-Osaa-1 vector, L146 is the abbreviated number of GATi-Osaa-2 vector, L127 is the abbreviated number of GATi-Osaa-3 vector, and L129 is the abbreviated number of GATi-Osaa-4 vector.

FIG. 5 shows GUS staining results of GATi-Osaa-1, GATi-Osaa-2 and GATi-Osaa-3 transgenic T0 seeds. Wherein, CK-is a wild control seed, CK+ is a positive control seed containing GUS gene line.

FIG. 6 is a chart of the phenotype observation of GATxGATi hybrid seeds. Wherein, a-1 and b-1 are visible light, and the hybrid seeds (GAT multiplied by GATi) containing GATi elements are blue after being dyed by GUS. a-2, b-2 are observed under green light excitation, and GAT seeds are shown to be red due to the red fluorescent seed screening element; the last row (GAT×GATi) of hybrid inbred progeny seeds in a-2 showed red fluorescence and blue GUS staining indicating both GAT and GATi elements; b-2 shows no red fluorescence but can be GUS stained, indicating that the GAT element and the GATi element in the hybridization material are separated and only the GATi element is contained. a-3 and b-3 are images overlapped under the bright field and the fluorescent field, and the images show that the seeds are the same batch of seeds.

FIG. 7 is a diagram showing phenotype observation of sterile line x (GATxGATi) hybrid seeds. Wherein, a-1, b-1 and c-1 are visible light, and the hybrid harvested seeds of GAT and GATi (sterile line X (GAT X GATi)) are blue after GUS staining. a-2, b-2 and c-2 are observed under green light excitation, and GAT seeds have red fluorescent seed screening elements which are shown as red; sterile line X (GAT X GATi) seeds in the last column of a-2 show that the seeds have red fluorescence and GUS staining is blue, and contain GAT and GATi elements, which shows that pollen containing GAT elements can be pollinated as male parent to sterile line in the presence of GATi; the last column in b-2, which shows no red fluorescence but can be GUS stained, shows that the GAT element and the GATi element in the hybridized material are separated, and only the GATi element is contained; the last column in c-2 shows neither red fluorescence nor GUS staining, and shows neither GAT element nor GATi element in the hybridization material, which is a nuclear male sterile line material obtained by new transformation. a-3, b-3 and c-3 are the superposition of pictures in the bright field and the fluorescent field, and show that the same seed is used.

FIG. 8 shows the results of molecular detection of sterile line x (GATxGATi) hybrid seed. Wherein a is a PCR diagram of 1-20 sample detection GAT elements, M is marker, -H ₂ O, ck-is wild type control, ck+ is genome DNA containing GAT element, 1-20 is seed DNA sample initiated by hybrid seed; b is PCR diagram of 1-20 samples detection GATi element, -H ₂ O, ck 1-is wild type control, ck1+ is genomic DNA containing GATi element, 1-20 is seed DNA sample initiated by hybrid seed; c is a PCR map of 21-44 samples of the detection GAT element; d is a PCR map of 21-44 samples for detecting GATi elements.

FIG. 9 is a diagram showing phenotype observation of sterile line x (GATxGATi-GFP) hybrid seeds. Wherein a-1, a-2, a-3, a-4 represent field, RFP fluorescence, GFP fluorescence, and trichromatic superposition results, respectively.

Detailed Description

The following examples are illustrative of the invention and are not intended to limit the scope of the invention. Modifications and substitutions to methods, procedures, or conditions of the present invention without departing from the spirit and nature of the invention are intended to be within the scope of the present invention.

The technical means used in the examples are conventional means well known to those skilled in the art unless otherwise indicated.

Example 1 Strain, plasmid acquisition and PCR primer Synthesis

(1) Strains and plasmids

The plant binary transformation vector pTCK303 contains hygromycin resistance gene, maize Ubi promoter and NOS terminator as skeleton vector. Coli (Escherichia coli) strain is DH5 a; the agrobacterium (Agrobacterim tumefacieus) strain is EHA105.

(2) PCR primer sequences

3 pairs of primers are designed, and the 5' ends of two adjacent upstream and downstream primers of the DNA fragments shown in SEQ ID No.1-3 are respectively amplified, wherein about 15 nucleotide sequences are repeated at the corresponding connection positions of the fragments or the vectors, so that the fragments or the vectors can be recombined and connected by using Gibson Assembly.

Osa1-F ATCGGGGAAATTCGAGCTTCTACAATAGAAGTTCTGCAAGTACCAGCG(SEQ ID No.8)；

Osa1-Rv1 ATTTTCAATCGATCTCACCCAGGCACTCCTGCAAT(SEQ ID No.9)；

Osa1-F2 GCCTGGGTGAGATCGATTGAAAATCTCGAAACAGCCGTGTC(SEQ ID No.10)；

Osa1-Rv2 TGGGTGAGGTCGACAGATCTGCTAGCGGTAAGTT(SEQ ID No.11)；

Osa1-F3 CTGTCGACCTCACCCAGGCACTCCTGCAAT(SEQ ID No.12)；

Osa1-Rv CTGCAGGTCGACTCTAGAGTCTACAATAGAAGTTCTGCAAGTACCAGCG(SEQ ID No.13)。

EXAMPLE 2 construction and transformation of plant expression vectors

The rice cDNA is used as a template, and the primers are used for PCR amplification of Osaa1 gene fragments 1 and 3. The amplification system and procedure were as follows:

the procedure is as follows: pre-denaturation at 94 ℃ for 5-10min, denaturation at 94 ℃ for 30s, annealing at 65 ℃ for 30s, extension at 72 ℃ for 30s,30-35 cycles, and extension at 72 ℃ for 5min; ending at 16 ℃.

The procedure is as follows: pre-denaturation at 94 ℃ for 5-10min, denaturation at 94 ℃ for 30s, annealing at 65 ℃ for 30s, extension at 72 ℃ for 30s,30-35 cycles, and extension at 72 ℃ for 5min; ending at 16 ℃.

The primer is used for PCR amplification of the Osaa1 gene fragment 2 by taking rice DNA as a template. The amplification system and procedure were as follows:

The procedure is as follows: pre-denaturation at 94 ℃ for 5-10min, denaturation at 94 ℃ for 30s, annealing at 62 ℃ for 30s, extension at 72 ℃ for 30s,30-35 cycles, and extension at 72 ℃ for 5min; ending at 16 ℃.

After agarose gel electrophoresis, E.Z.N.A was used.The PCR product fragments were recovered from Extraction kit (Omega, the same applies below) and were respectively 310bp, 502bp and 310bp in size, and were designated as Osaai-1-1 (SEQ ID No. 1), osaai-1-2 (SEQ ID No. 2) and Osaai-1-3 (SEQ ID No. 3), respectively.

Vector plasmid PTCK303 was digested with SacI+BamHI and subjected to agarose gel electrophoresis, followed by E.Z.N.A.The Extraction kit (Omega, supra) was recovered and a band of about 15kb in size, giving a linear fragment of PTCK303 (FIG. 1).

The SacI+BamHI double cleavage reaction system is as follows:

2) 2x Lightening Cloning Kit connection kitThree fragments of Osaai-1-1, osaai-1-2 and Osaai-1-3 were ligated to PTCK303 vector as follows:

and (3) connection procedure: 30min at 50 ℃.

The ligation product is transformed into E.coli DH5 alpha competent cells, the recombinant plasmid is subjected to BstXI and HindIII double digestion, the size of a target fragment accords with the expectations (figure 2), and the recombinant is subjected to sequencing verification to be correct, namely the Osaai-1 GATi vector constructed by taking Rice Intron as a loop, and the vector is named GATi-Osaai-1 (SEQ ID No. 4).

In order to cover the OsAA region, three other GATi vectors, named GATi-Osaai-2 (SEQ ID No. 5) and GATi-Osaai-3 (SEQ ID No. 6) and GATi-Osaai-4 (SEQ ID No. 21), were constructed by a similar vector construction method.

Wherein, GATi-Osaai-1 (SEQ ID No. 4), GATi-Osaai-2 (SEQ ID No. 5) and GATi-Osaai-3 (SEQ ID No. 6) are the "stem" structures of the GTai vector aiming at the gene sequences of different regions on the Osaa gene coding frame.

Meanwhile, the GUS expression cassette in pTCK303 vector can be replaced by a fluorescent protein GFP expression cassette, and the obtained GATi vector is named GATi-Osaai-1GFP (SEQ ID No. 18), GATi-Osaai-2GFP (SEQ ID No. 19) or GATi-Osaai-3GFP (SEQ ID No. 20).

Example 3 identification of Agrobacterium-transformed Rice and transgenic plants

The GATi-Osaai-1 recombinant plasmid prepared in the example 2 is introduced into an agrobacterium EHA105 strain, and transformed into flower 11 callus of japonica rice, and subjected to hygromycin resistance screening, differentiation and rooting to obtain 22 regenerated transgenic lines, and the hygromycin resistance genes transferred into the transgenic lines are identified by PCR, so that the result shows that 21 PCR positive plants (figure 3) are transplanted into soil, and 20 positive plants survive.

The GATi-Osaai-2 recombinant plasmid prepared in the example 2 is introduced into an agrobacterium EHA105 strain, and transformed into flower 11 callus of japonica rice, and 25 transgenic positive plants are obtained through tissue culture screening and transplanted to a greenhouse, and 18 plants survive. The GATi-Osaai-3 is used for transforming the flowers 11 in japonica rice to obtain 30 transgenic positive plants, and the transgenic positive plants are transplanted to a greenhouse to survive 27 plants. The GATi-Osaai-4 is used for transforming the flowers 11 in japonica rice to obtain 15 positive transgenic plants, and the 15 positive transgenic plants are transplanted to a greenhouse to survive 12 plants.

Example 4 detection of the expression level of plant alpha-amylase Gene

To further determine whether the interfering vector fragment resulted in a decrease in the expression level of the plant alpha-amylase gene, the present example examined the expression level of the alpha-amylase gene.

The T1 generation 3 strains of 5 transgenic lines 3-2, 4-2, 8-2, 13-1 and 13-2 of GATi-Osaai-1 of example 3 were selected; and T1 generation 3 strains of 4 transgenic lines 3-1, 3-2, 5-1 and 5-2 of GATi-Osaai-2; t1 generation 3 strains of 5 transgenic lines 2-1, 3-2, 10-1 and 15-2 of GATi-Osaai-3; the T1 generation 3 strains of the 4 transgenic strains 1-1, 1-2, 6-1 and 6-2 of GATi-Osaai-4 are detected, the medium flower 11 is used as a negative control, two transgenic strains of the Osaa over-expression vector are used as a positive control, total RNA is extracted, and reverse transcription is carried out to obtain cDNA.

In a Thermo Picoreal 96 real-time fluorescence quantitative PCR system, the expression of an alpha-amylase gene is detected by taking an action gene as an internal reference. The reaction system and the procedure were as follows:

RT-PCR primer sequences:

OsaaqRT-F6 TCGCCTGTTGATGTATGCATGTTC(SEQ ID No.14)；

OsaaqRT-R6 GTGTATCACATAGTATTACATAGCTCCAACATG(SEQ ID No.15)；

Actin-F AGCATGAAGATCAAGGTGGTC(SEQ ID No.16)；

Actin-R GCCTTGGCAATCCACATC(SEQ ID No.17)。

the fluorescent quantitative PCR reaction system and the procedure are as follows:

the procedure is as follows: pre-denaturation at 94℃for 7min, denaturation at 94℃for 15s, annealing at 62℃for 15s; extension at 72℃for 15s (fluorescence detection); 40 cycles; preserving heat at 60 ℃ for 30s. Dissolution profile procedure: 60-95 ℃ (0.2 ℃ per rise, hold for 1 s); preserving heat at 20 ℃ for 10s; and (5) ending.

The procedure is as follows: pre-denaturation at 94℃for 7min, denaturation at 94℃for 15s, annealing at 55℃for 15s; extension at 72℃for 15s (fluorescence detection); 40 cycles. Dissolution profile procedure: 60-95 ℃ (0.2 ℃ per rise, hold for 1 s); preserving heat at 20 ℃ for 10s; and (5) ending.

The fluorescent quantitative PCR amplification result shows that the melting curve peaks of the target gene and the reference gene are single peak shape and sharp shape. The melting temperature of each sample is indicated to be uniform, the specificity of the amplified product is good, and a non-specific double-stranded DNA product or primer dimer is not found. The amplification curve is smooth and stable, the S-shaped curve of the fluorescence absorption spectrum is perfect in shape, and the quantitative detection requirement is met. The results of relative quantification (Liva K J, schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and 2 (-Delta Delta Delta C (T)) Methods,2001,25 (4): 402-408.) using the comparative threshold Method showed that the expression of the alpha-amylase gene was strongly down-regulated in the transgenic lines of GATi-Osaai-1-3, with the majority of the lines being 3-20% of the wild-type control and the minority of the lines being 30-65% of the wild-type control, but the expression of the alpha-amylase gene was not significantly changed in the transgenic lines of GATi-Osaai-4, with the expression level shown in FIG. 4. It is demonstrated that the interfering fragment of the GATi-Osaai-1-3 vector has a strong inhibitory effect on the expression of the alpha-amylase gene in addition to GATi-Osaai-4. The results show that the interference fragments of 3 GATi vectors and the plant binary transformation vector containing the interference fragments can be successfully used for inhibiting the expression of plant alpha-amylase genes.

EXAMPLE 5 GUS staining identification of GATi-Osaa-1, GATi-Osaa-2 and GATi-Osaa-3 transgenic seeds

The seeds of the transgenic materials GATi-Osaai-1, GATi-Osaa-2 and GATi-Osaa-3 are transversely cut into two halves, and the embryo-free half is taken for GUS staining, and the staining working solution is prepared according to the recommended concentration ratio of X-gluc solution/staining buffer=1/50 of GUS staining kit (Zhongkeruitai, cat#: RTU 4032). After immersing the seeds in GUS staining broth and incubating in a 37℃incubator for 6 hours, 3 vector seeds were either transgene positive if stained blue-black, transgene negative or transgene silenced if staining was unchanged (see FIG. 5).

Example 6 GFP fluorescence identification of GATi-Osaa-1GFP, GATi-Osaa-2GFP and GATi-Osaa-3GFP transgenic seed

The GATi-Osaai-1gfp, GATi-Osaa-2gfp and GATi-Osaa-3gfp transgenic material seeds are irradiated under blue fluorescence excitation light, and the seeds with green fluorescence are GATi-Osaai-1gfp, GATi-Osaa-2gfp or GATi-Osaa-3gfp transgenic positive seeds, and the non-fluorescence is transgenic negative or transgenic silent seeds.

EXAMPLE 7 hybridization of GATi with Nuclear Male sterile maintainer line as male parent transformation identification 1

The F1 seed is obtained by hybridization with a nuclear male sterile maintainer line (hereinafter referred to as GAT) containing pollen abortive gene Osaa as female parent and GATi-Osaa-1, GATi-Osaa-2 and GATi-Osaa-3 transgenic positive lines as male parent. Through PCR molecular detection, the F1 seed contains two genotypes, one of which is GATi and GAT vector-containing, and can be used for GUS staining and displaying red fluorescent seeds; the other was a GATi-only vector, GUS-only stained red-free fluorescent seed, as shown in FIG. 6.

Sterile material 1907 is used as a female parent (for convenient hybridization, sterile line is used as a female parent), F1 strain containing two vectors is used as a male parent for hybridization, and whether the male parent can transmit pollen containing GAT vectors or not is verified. GUS staining was performed on the obtained 44-grain hybrid, and fluorescence observation was performed, which revealed that there were 27 grains containing GAT (red fluorescent seeds) and GATi (GUS staining); only 0 grains containing GAT (red fluorescent seeds without GUS staining); 16 grains containing GATi (GUS stained red-less fluorescent seeds); none contained 1 grain (no red fluorescent seeds nor GUS staining). As shown in fig. 7.

PCR detection is carried out after the hybrid seedlings develop, and the result shows that 27 strains containing GAT and GATi; only GAT-containing strain 0; 16 strains containing only GATi; 1 strain which is not contained in the whole cell line, as shown in FIG. 8. The above results indicate that the strain containing both vectors, in which the GATi is active, pollinates other materials, can be obtained such that the transgenic pollen containing the recessive nuclear male sterile maintainer element is not completely degraded or killed. F2 is obtained by selfing the newly obtained hybrid seeds, and the expected plants are obtained by molecular detection and F2 separation. The GATi has good effect, and achieves the aim of pollinating the nuclear male sterile maintainer line as a male parent.

EXAMPLE 8 hybridization of GATi with Nuclear Male sterile maintainer line as male parent transformation identification 2

The GUS gene expression cassette in the original GATi-Osaai-1 (SEQ ID No. 4) vector is replaced by a GFP fluorescent gene expression cassette, and the construction is completed and named as GATi-Osaa-1-GFP (SEQ ID No. 7). The GATi-Osaa-1-GFP transgenic seed was clearly seen to contain green fluorescence upon blue light excitation. The GATi-GFP transgene was crossed with a nuclear male sterile maintainer line to obtain F1 hybrids. The F1 seeds are detected to have two genotypes by PCR, one of which is seeds containing GATi-GFP and GAT vector and containing green fluorescence and red fluorescence; the other was only GATi-GFP vector, showing green fluorescent seeds. Sterile material 1907 is used as a female parent (for convenient hybridization, sterile line is used as a female parent), a strain containing two vectors is used as a male parent for hybridization, and whether the male parent can transmit pollen containing GAT vectors or not is verified. Fluorescence detection was performed on the hybrid seeds, and the results showed that the hybrid seeds contained 3 types of seeds, including GAT (red fluorescent seeds) and GATi-GFP (green fluorescent seeds); GATi-GFP-containing (green fluorescent non-red fluorescent seeds); none (no red nor green fluorescent seeds) as shown in fig. 9.

The phenotype is consistent with the genotype result through PCR detection. The above results indicate that a strain comprising both vectors can be active in that transgenic pollen containing elements of the nuclear male sterile maintainer line is not entirely degraded or killed and can pollinate other materials. F2 is obtained by selfing the F1 seeds, and the F2 is separated to obtain the expected plants through molecular detection. Showing that GATi has good effects.

Example 9 application of GATi in corn

Using a similar procedure in rice, F1 seeds were obtained in maize where GAT hybridized with GATi. Similarly, by PCR molecular detection, F1 seeds contain two genotypes, one is seeds containing GATi and GAT vectors; the other is seeds containing only the GATi vector. And (3) taking B104 as a female parent and taking a strain containing two vectors as a male parent for hybridization, and verifying whether the male parent can transmit pollen containing the GAT vector. Through the primary analysis of red fluorescent color development and GUS dyeing or green fluorescent color development and the subsequent PCR further detection, the strain containing the silencing element mark and the recessive nuclear male sterile maintainer line mark can lead the transgenic pollen containing the recessive nuclear male sterile maintainer line element not to be completely degraded or killed, thereby having activity and being capable of pollinating other materials. F2 is obtained by selfing the F1 seeds, and the F2 is separated to obtain the expected plants through molecular detection. Showing that GATi has good effects.

Example 10 application of GATi in wheat

Using a similar procedure in rice, F1 seeds were obtained in wheat where GAT hybridized with GATi. Similarly, by PCR molecular detection, F1 seeds contain two genotypes, one is seeds containing GATi and GAT vectors; the other is seeds containing only the GATi vector. Hybridization is carried out by taking elytrigia pumila 22 as a female parent and a strain containing two vectors as a male parent, and whether the male parent can transmit pollen containing GAT vectors or not is verified. Through the primary analysis of red fluorescent color development and GUS dyeing or green fluorescent color development and the subsequent PCR further detection, the strain containing the silencing element mark and the recessive nuclear male sterile maintainer line mark can lead the transgenic pollen containing the recessive nuclear male sterile maintainer line element not to be completely degraded or killed, thereby having activity and being capable of pollinating other materials. F2 is obtained by selfing the F1 seeds, and the F2 is separated to obtain the expected plants through molecular detection. Showing that GATi has good effects.

EXAMPLE 11 application of GATi in rape

By using a similar method in rice, F1 seeds were obtained in which GAT in rape was hybridized with GATi. Similarly, by PCR molecular detection, F1 seeds contain two genotypes, one is seeds containing GATi and GAT vectors; the other is seeds containing only the GATi vector. The sterile line is used as a female parent, the strain containing the two vectors is used as a male parent for hybridization, and whether the male parent can transmit pollen containing the GAT vector or not is verified. Through the primary analysis of red fluorescent color development and GUS dyeing or green fluorescent color development and the subsequent PCR further detection, the strain containing the silencing element mark and the recessive nuclear male sterile maintainer line mark can lead the transgenic pollen containing the recessive nuclear male sterile maintainer line element not to be completely degraded or killed, thereby having activity and being capable of pollinating other materials. F2 is obtained by selfing the F1 seeds, and the F2 is separated to obtain the expected plants through molecular detection. Showing that GATi has good effects.

EXAMPLE 12 application of GATi in cotton

Using a similar method in rice, F1 seeds were obtained in cotton where GAT hybridized with GATi. Similarly, by PCR molecular detection, F1 seeds contain two genotypes, one is seeds containing GATi and GAT vectors; the other is seeds containing only the GATi vector. The sterile line is used as a female parent, the strain containing the two vectors is used as a male parent for hybridization, and whether the male parent can transmit pollen containing the GAT vector or not is verified. Through the primary analysis of red fluorescent color development and GUS dyeing or green fluorescent color development and the subsequent PCR further detection, the strain containing the silencing element mark and the recessive nuclear male sterile maintainer line mark can lead the transgenic pollen containing the recessive nuclear male sterile maintainer line element not to be completely degraded or killed, thereby having activity and being capable of pollinating other materials. F2 is obtained by selfing the F1 seeds, and the F2 is separated to obtain the expected plants through molecular detection. Showing that GATi has good effects.

While the invention has been described in detail in the foregoing general description and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that modifications and improvements can be made thereto. Accordingly, such modifications or improvements may be made without departing from the spirit of the invention and are intended to be within the scope of the invention as claimed.

Sequence listing

<110> Hainan Beunder Rice Gene technology Co., ltd

<120> a method for transferring a nuclear male sterile maintainer line

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cctgcgccaa tcttcattgt taccctctca tcaatctctg ccgcatataa atttccctct 180

gctttcagta tcttgatctt gctacggcaa tggatctttt ggcgatttct aatagaaatt 240

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actgccgtgg aactgtcata tttgattatg aaattttagc tctagaaaat atttgatcat 180

caatgtcaag actttatgac cttgcaaaat acatttccta attgagaaca gggtaaaatt 240

atgaactatg cctctgaacc ttcatacaca ggcagcacat tttttgttgt aaaattcatc 300

ttaatatcag cggaaagact ggaccagaga aagaaaaagt taagacaggc atatactctt 360

gatcctctaa aagagatgag gcggtacaat gatcaaccat gaacattaaa gtgatacgtg 420

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tactgaaagc agagggaaat ttatatgcgg cagagattga tgagagggta acaatgaaga 180

ttggcgcagg acattttgag ccaagcggcc ccacaaactg ggtagttgct gccgagggac 240

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catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct 60

tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga 120

ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt 180

gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaacgtgaaa 240

tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc 300

agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta 360

acgatcagtt cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg 420

aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca 480

ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata 540

cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg 600

tttgtgtgaa caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg 660

aaaacggcaa gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc 720

gcagcgtaat gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc 780

atgtcgcgca agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg 840

tcagcgttga actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg 900

ggactttgca agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac 960

tgtgcgtcac agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc 1020

ggtcagtggc agtgaagggc gaacagttcc tgattaacca caaaccgttc tactttactg 1080

gctttggtcg tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg 1140

tgcacgacca cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc 1200

cttacgctga agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa 1260

ctgctgctgt cggctttaac ctctctttag gcattggttt cgaagcgggc aacaagccga 1320

aagaactgta cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga 1380

ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca 1440

acgaaccgga tacccgtccg caagtgcacg ggaatatttc gccactggcg gaagcaacgc 1500

gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca 1560

ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg 1620

tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc 1680

aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc 1740

tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt 1800

atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg 1860

attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc 1920

gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact 1980

tcggtgaaaa accgcagcag ggaggcaaac aagctagcca ccaccaccac caccacgtgt 2040

gaattacagg tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc 2100

ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac 2160

gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg 2220

attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac 2280

taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag 2340

tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg 2400

gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc 2460

acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt 2520

cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt 2580

tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg 2640

cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc 2700

cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg 2760

ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag 2820

gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac 2880

agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg 2940

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aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg 3120

cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga 3180

aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg 3240

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acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc 3420

gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg 3480

cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc 3540

ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac 3600

agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag 3660

gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca 3720

tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt 3780

ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg 3840

ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact 3900

tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag 3960

ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc 4020

tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg 4080

tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg 4140

ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg 4200

ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc 4260

aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat 4320

gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc 4380

aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc 4440

ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct 4500

gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat 4560

gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg 4620

tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct 4680

gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa 4740

ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag 4800

gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg 4860

caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg 4920

tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat 4980

gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag 5040

cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt 5100

tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt 5160

tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc 5220

gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag 5280

cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag 5340

cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt 5400

agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta 5460

gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac 5520

cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc 5580

gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc 5640

gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg 5700

ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac 5760

cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg 5820

caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac 5880

attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg 5940

tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt 6000

tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa 6060

ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc 6120

tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct 6180

ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc 6240

cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg 6300

acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 6360

agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc 6420

acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg 6480

agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 6540

aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 6600

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 6660

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 6720

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 6780

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 6840

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 6900

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 6960

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 7020

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 7080

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 7140

tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 7200

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 7260

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 7320

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 7380

attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt 7440

ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc 7500

ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg 7560

ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg 7620

tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa 7680

tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca 7740

tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc 7800

gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc 7860

tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg 7920

gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga 7980

acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg 8040

gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc 8100

ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc 8160

agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc 8220

tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt 8280

tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt 8340

tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa 8400

cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc 8460

cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct 8520

tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga 8580

ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg 8640

tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg 8700

aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa 8760

ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat 8820

atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc 8880

acacattatt atggagaaac tcgagcttgt cgatcgacag atccggtcgg catctactct 8940

atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta 9000

cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc 9060

cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat 9120

tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga 9180

gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca 9240

tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga 9300

acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt 9360

tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca 9420

tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc 9480

agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac 9540

cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga 9600

tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt 9660

cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctaaact 9720

ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat 9780

aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc 9840

ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga 9900

cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt 9960

tcatatctca ttgccccccc ggatctgcga aagctcgaga gagatagatt tgtagagaga 10020

gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggt 10080

cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat 10140

ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg 10200

ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat 10260

cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac 10320

agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct 10380

caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt 10440

cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 10500

ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg 10560

catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct 10620

tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc 10680

ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt 10740

tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc 10800

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 10860

gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 10920

ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 10980

cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcc ccgatctagt 11040

aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct 11100

atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac 11160

gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata 11220

atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa 11280

cgatcgggga aattcgagct tctacaatag aagttctgca agtaccagcg cggcaagtct 11340

acgatgacac ttcccagacc ttgtaatcct gtccctcggc agcaactacc cagtttgtgg 11400

ggccgcttgg ctcaaaatgt cctgcgccaa tcttcattgt taccctctca tcaatctctg 11460

ccgcatataa atttccctct gctttcagta tcttgatctt gctacggcaa tggatctttt 11520

ggcgatttct aatagaaatt aatttagcaa tctctggctg taaatgcgaa aatatatgat 11580

catagaagat tgcaggagtg cctgggtgag atcgattgaa aatctcgaaa cagccgtgtc 11640

atagtcaatc attaggtgtt ataggaacaa tcaaaggttt tttcaagtgt taatcttcat 11700

actaatatat acagtgggta ctctttatct actgccgtgg aactgtcata tttgattatg 11760

aaattttagc tctagaaaat atttgatcat caatgtcaag actttatgac cttgcaaaat 11820

acatttccta attgagaaca gggtaaaatt atgaactatg cctctgaacc ttcatacaca 11880

ggcagcacat tttttgttgt aaaattcatc ttaatatcag cggaaagact ggaccagaga 11940

aagaaaaagt taagacaggc atatactctt gatcctctaa aagagatgag gcggtacaat 12000

gatcaaccat gaacattaaa gtgatacgtg gaacatgaga acacaaataa ttgtcactgg 12060

aacataaata caaaaaggtt tgtagtaact taccgctagc agatctgtcg acctcaccca 12120

ggcactcctg caatcttcta tgatcatata ttttcgcatt tacagccaga gattgctaaa 12180

ttaatttcta ttagaaatcg ccaaaagatc cattgccgta gcaagatcaa gatactgaaa 12240

gcagagggaa atttatatgc ggcagagatt gatgagaggg taacaatgaa gattggcgca 12300

ggacattttg agccaagcgg ccccacaaac tgggtagttg ctgccgaggg acaggattac 12360

aaggtctggg aagtgtcatc gtagacttgc cgcgctggta cttgcagaac ttctattgta 12420

gactctagag tcgacctgca gaagtaacac caaacaacag ggtgagcatc gacaaaagaa 12480

acagtaccaa gcaaataaat agcgtatgaa ggcagggcta aaaaaatcca catatagctg 12540

ctgcatatgc catcatccaa gtatatcaag atcaaaataa ttataaaaca tacttgttta 12600

ttataataga taggtactca aggttagagc atatgaatag atgctgcata tgccatcatg 12660

tatatgcatc agtaaaaccc acatcaacat gtatacctat cctagatcga tatttccatc 12720

catcttaaac tcgtaactat gaagatgtat gacacacaca tacagttcca aaattaataa 12780

atacaccagg tagtttgaaa cagtattcta ctccgatcta gaacgaatga acgaccgccc 12840

aaccacacca catcatcaca accaagcgaa caaaaagcat ctctgtatat gcatcagtaa 12900

aacccgcatc aacatgtata cctatcctag atcgatattt ccatccatca tcttcaattc 12960

gtaactatga atatgtatgg cacacacata cagatccaaa attaataaat ccaccaggta 13020

gtttgaaaca gaattctact ccgatctaga acgaccgccc aaccagacca catcatcaca 13080

accaagacaa aaaaaagcat gaaaagatga cccgacaaac aagtgcacgg catatattga 13140

aataaaggaa aagggcaaac caaaccctat gcaacgaaac aaaaaaaatc atgaaatcga 13200

tcccgtctgc ggaacggcta gagccatccc aggattcccc aaagagaaac actggcaagt 13260

tagcaatcag aacgtgtctg acgtacaggt cgcatccgtg tacgaacgct agcagcacgg 13320

atctaacaca aacacggatc taacacaaac atgaacagaa gtagaactac cgggccctaa 13380

ccatggaccg gaacgccgat ctagagaagg tagagagggg ggggggggga ggacgagcgg 13440

cgtaccttga agcggaggtg ccgacgggtg gatttggggg agatctggtt gtgtgtgtgt 13500

gcgctccgaa caacacgagg ttggggaaag agggtgtgga gggggtgtct atttattacg 13560

gcgggcgagg aagggaaagc gaaggagcgg tgggaaagga atcccccgta gctgccggtg 13620

ccgtgagagg aggaggaggc cgcctgccgt gccggctcac gtctgccgct ccgccacgca 13680

atttctggat gccgacagcg gagcaagtcc aacggtggag cggaactctc gagaggggtc 13740

cagaggcagc gacagagatg ccgtgccgtc tgcttcgctt ggcccgacgc gacgctgctg 13800

gttcgctggt tggtgtccgt tagactcgtc gacggcgttt aacaggctgg cattatctac 13860

tcgaaacaag aaaaatgttt ccttagtttt tttaatttct taaagggtat ttgtttaatt 13920

tttagtcact ttattttatt ctattttata tctaaattat taaataaaaa aactaaaata 13980

gagttttagt tttcttaatt tagaggctaa aatagaataa aatagatgta ctaaaaaaat 14040

tagtctataa aaaccattaa ccctaaaccc taaatggatg tactaataaa atggatgaag 14100

tattatatag gtgaagctat ttgcaaaaaa aaaggagaac acatgcacac taaaaagata 14160

aaactgtaga gtcctgttgt caaaatactc aattgtcctt tagaccatgt ctaactgttc 14220

atttatatga ttctctaaaa cactgatatt attgtagtac tatagattat attattcgta 14280

gagtaaagtt taaatatatg tataaagata gataaactgc acttcaaaca agtgtgacaa 14340

aaaaaatatg tggtaatttt ttataactta gacatgcaat gctcattatc tctagagagg 14400

ggcacgaccg ggtcacgctg cacaagcttg gcactggccg tcgttttaca acgtcgtgac 14460

tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 14520

tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 14580

ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttagc 14640

ttcatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa 14700

cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg 14760

gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg 14820

gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca 14880

gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat 14940

cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat 15000

ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag 15060

caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct 15120

tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact 15180

cttgac 15186

<210> 5

<211> 15066

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 5

catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct 60

tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga 120

ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt 180

gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaacgtgaaa 240

tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc 300

agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta 360

acgatcagtt cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg 420

aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca 480

ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata 540

cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg 600

tttgtgtgaa caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg 660

aaaacggcaa gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc 720

gcagcgtaat gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc 780

atgtcgcgca agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg 840

tcagcgttga actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg 900

ggactttgca agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac 960

tgtgcgtcac agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc 1020

ggtcagtggc agtgaagggc gaacagttcc tgattaacca caaaccgttc tactttactg 1080

gctttggtcg tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg 1140

tgcacgacca cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc 1200

cttacgctga agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa 1260

ctgctgctgt cggctttaac ctctctttag gcattggttt cgaagcgggc aacaagccga 1320

aagaactgta cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga 1380

ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca 1440

acgaaccgga tacccgtccg caagtgcacg ggaatatttc gccactggcg gaagcaacgc 1500

gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca 1560

ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg 1620

tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc 1680

aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc 1740

tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt 1800

atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg 1860

attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc 1920

gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact 1980

tcggtgaaaa accgcagcag ggaggcaaac aagctagcca ccaccaccac caccacgtgt 2040

gaattacagg tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc 2100

ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac 2160

gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg 2220

attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac 2280

taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag 2340

tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg 2400

gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc 2460

acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt 2520

cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt 2580

tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg 2640

cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc 2700

cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg 2760

ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag 2820

gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac 2880

agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg 2940

catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac 3000

gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct 3060

aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg 3120

cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga 3180

aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg 3240

cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg 3300

tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga 3360

acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc 3420

gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg 3480

cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc 3540

ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac 3600

agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag 3660

gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca 3720

tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt 3780

ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg 3840

ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact 3900

tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag 3960

ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc 4020

tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg 4080

tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg 4140

ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg 4200

ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc 4260

aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat 4320

gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc 4380

aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc 4440

ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct 4500

gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat 4560

gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg 4620

tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct 4680

gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa 4740

ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag 4800

gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg 4860

caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg 4920

tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat 4980

gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag 5040

cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt 5100

tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt 5160

tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc 5220

gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag 5280

cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag 5340

cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt 5400

agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta 5460

gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac 5520

cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc 5580

gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc 5640

gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg 5700

ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac 5760

cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg 5820

caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac 5880

attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg 5940

tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt 6000

tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa 6060

ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc 6120

tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct 6180

ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc 6240

cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg 6300

acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 6360

agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc 6420

acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg 6480

agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 6540

aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 6600

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 6660

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 6720

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 6780

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 6840

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 6900

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 6960

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 7020

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 7080

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 7140

tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 7200

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 7260

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 7320

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 7380

attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt 7440

ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc 7500

ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg 7560

ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg 7620

tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa 7680

tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca 7740

tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc 7800

gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc 7860

tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg 7920

gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga 7980

acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg 8040

gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc 8100

ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc 8160

agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc 8220

tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt 8280

tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt 8340

tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa 8400

cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc 8460

cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct 8520

tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga 8580

ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg 8640

tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg 8700

aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa 8760

ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat 8820

atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc 8880

acacattatt atggagaaac tcgagcttgt cgatcgacag atccggtcgg catctactct 8940

atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta 9000

cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc 9060

cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat 9120

tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga 9180

gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca 9240

tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga 9300

acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt 9360

tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca 9420

tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc 9480

agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac 9540

cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga 9600

tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt 9660

cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctaaact 9720

ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat 9780

aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc 9840

ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga 9900

cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt 9960

tcatatctca ttgccccccc ggatctgcga aagctcgaga gagatagatt tgtagagaga 10020

gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggt 10080

cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat 10140

ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg 10200

ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat 10260

cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac 10320

agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct 10380

caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt 10440

cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 10500

ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg 10560

catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct 10620

tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc 10680

ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt 10740

tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc 10800

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 10860

gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 10920

ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 10980

cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcc ccgatctagt 11040

aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct 11100

atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac 11160

gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata 11220

atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa 11280

cgatcgggga aattcgagct tctataaatg tgacagcacg cgaaggccac caacctaaca 11340

ctccaggggg ttttcctttt tcatcagaca gacgccagta ctcagatctt tccagtgcag 11400

agtgaagtat tcctttcgtg gtaacatcaa atgcaccagc agttccattt gtagcattta 11460

tccaatcaac tattctctgc ctgtgggcat cttgattata atccatttca ccataggtgt 11520

aactgagaga atcccagtac tctcctactg atcgattgaa aatctcgaaa cagccgtgtc 11580

atagtcaatc attaggtgtt ataggaacaa tcaaaggttt tttcaagtgt taatcttcat 11640

actaatatat acagtgggta ctctttatct actgccgtgg aactgtcata tttgattatg 11700

aaattttagc tctagaaaat atttgatcat caatgtcaag actttatgac cttgcaaaat 11760

acatttccta attgagaaca gggtaaaatt atgaactatg cctctgaacc ttcatacaca 11820

ggcagcacat tttttgttgt aaaattcatc ttaatatcag cggaaagact ggaccagaga 11880

aagaaaaagt taagacaggc atatactctt gatcctctaa aagagatgag gcggtacaat 11940

gatcaaccat gaacattaaa gtgatacgtg gaacatgaga acacaaataa ttgtcactgg 12000

aacataaata caaaaaggtt tgtagtaact taccgctagc agatctgtcg accagtagga 12060

gagtactggg attctctcag ttacacctat ggtgaaatgg attataatca agatgcccac 12120

aggcagagaa tagttgattg gataaatgct acaaatggaa ctgctggtgc atttgatgtt 12180

accacgaaag gaatacttca ctctgcactg gaaagatctg agtactggcg tctgtctgat 12240

gaaaaaggaa aaccccctgg agtgttaggt tggtggcctt cgcgtgctgt cacatttata 12300

gactctagag tcgacctgca gaagtaacac caaacaacag ggtgagcatc gacaaaagaa 12360

acagtaccaa gcaaataaat agcgtatgaa ggcagggcta aaaaaatcca catatagctg 12420

ctgcatatgc catcatccaa gtatatcaag atcaaaataa ttataaaaca tacttgttta 12480

ttataataga taggtactca aggttagagc atatgaatag atgctgcata tgccatcatg 12540

tatatgcatc agtaaaaccc acatcaacat gtatacctat cctagatcga tatttccatc 12600

catcttaaac tcgtaactat gaagatgtat gacacacaca tacagttcca aaattaataa 12660

atacaccagg tagtttgaaa cagtattcta ctccgatcta gaacgaatga acgaccgccc 12720

aaccacacca catcatcaca accaagcgaa caaaaagcat ctctgtatat gcatcagtaa 12780

aacccgcatc aacatgtata cctatcctag atcgatattt ccatccatca tcttcaattc 12840

gtaactatga atatgtatgg cacacacata cagatccaaa attaataaat ccaccaggta 12900

gtttgaaaca gaattctact ccgatctaga acgaccgccc aaccagacca catcatcaca 12960

accaagacaa aaaaaagcat gaaaagatga cccgacaaac aagtgcacgg catatattga 13020

aataaaggaa aagggcaaac caaaccctat gcaacgaaac aaaaaaaatc atgaaatcga 13080

tcccgtctgc ggaacggcta gagccatccc aggattcccc aaagagaaac actggcaagt 13140

tagcaatcag aacgtgtctg acgtacaggt cgcatccgtg tacgaacgct agcagcacgg 13200

atctaacaca aacacggatc taacacaaac atgaacagaa gtagaactac cgggccctaa 13260

ccatggaccg gaacgccgat ctagagaagg tagagagggg ggggggggga ggacgagcgg 13320

cgtaccttga agcggaggtg ccgacgggtg gatttggggg agatctggtt gtgtgtgtgt 13380

gcgctccgaa caacacgagg ttggggaaag agggtgtgga gggggtgtct atttattacg 13440

gcgggcgagg aagggaaagc gaaggagcgg tgggaaagga atcccccgta gctgccggtg 13500

ccgtgagagg aggaggaggc cgcctgccgt gccggctcac gtctgccgct ccgccacgca 13560

atttctggat gccgacagcg gagcaagtcc aacggtggag cggaactctc gagaggggtc 13620

cagaggcagc gacagagatg ccgtgccgtc tgcttcgctt ggcccgacgc gacgctgctg 13680

gttcgctggt tggtgtccgt tagactcgtc gacggcgttt aacaggctgg cattatctac 13740

tcgaaacaag aaaaatgttt ccttagtttt tttaatttct taaagggtat ttgtttaatt 13800

tttagtcact ttattttatt ctattttata tctaaattat taaataaaaa aactaaaata 13860

gagttttagt tttcttaatt tagaggctaa aatagaataa aatagatgta ctaaaaaaat 13920

tagtctataa aaaccattaa ccctaaaccc taaatggatg tactaataaa atggatgaag 13980

tattatatag gtgaagctat ttgcaaaaaa aaaggagaac acatgcacac taaaaagata 14040

aaactgtaga gtcctgttgt caaaatactc aattgtcctt tagaccatgt ctaactgttc 14100

atttatatga ttctctaaaa cactgatatt attgtagtac tatagattat attattcgta 14160

gagtaaagtt taaatatatg tataaagata gataaactgc acttcaaaca agtgtgacaa 14220

aaaaaatatg tggtaatttt ttataactta gacatgcaat gctcattatc tctagagagg 14280

ggcacgaccg ggtcacgctg cacaagcttg gcactggccg tcgttttaca acgtcgtgac 14340

tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 14400

tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 14460

ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttagc 14520

ttcatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa 14580

cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg 14640

gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg 14700

gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca 14760

gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat 14820

cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat 14880

ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag 14940

caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct 15000

tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact 15060

cttgac 15066

<210> 6

<211> 15206

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 6

catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct 60

tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga 120

ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt 180

gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaacgtgaaa 240

tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc 300

agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta 360

acgatcagtt cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg 420

aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca 480

ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata 540

cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg 600

tttgtgtgaa caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg 660

aaaacggcaa gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc 720

gcagcgtaat gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc 780

atgtcgcgca agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg 840

tcagcgttga actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg 900

ggactttgca agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac 960

tgtgcgtcac agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc 1020

ggtcagtggc agtgaagggc gaacagttcc tgattaacca caaaccgttc tactttactg 1080

gctttggtcg tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg 1140

tgcacgacca cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc 1200

cttacgctga agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa 1260

ctgctgctgt cggctttaac ctctctttag gcattggttt cgaagcgggc aacaagccga 1320

aagaactgta cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga 1380

ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca 1440

acgaaccgga tacccgtccg caagtgcacg ggaatatttc gccactggcg gaagcaacgc 1500

gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca 1560

ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg 1620

tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc 1680

aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc 1740

tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt 1800

atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg 1860

attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc 1920

gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact 1980

tcggtgaaaa accgcagcag ggaggcaaac aagctagcca ccaccaccac caccacgtgt 2040

gaattacagg tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc 2100

ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac 2160

gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg 2220

attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac 2280

taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag 2340

tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg 2400

gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc 2460

acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt 2520

cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt 2580

tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg 2640

cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc 2700

cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg 2760

ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag 2820

gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac 2880

agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg 2940

catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac 3000

gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct 3060

aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg 3120

cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga 3180

aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg 3240

cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg 3300

tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga 3360

acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc 3420

gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg 3480

cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc 3540

ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac 3600

agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag 3660

gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca 3720

tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt 3780

ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg 3840

ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact 3900

tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag 3960

ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc 4020

tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg 4080

tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg 4140

ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg 4200

ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc 4260

aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat 4320

gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc 4380

aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc 4440

ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct 4500

gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat 4560

gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg 4620

tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct 4680

gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa 4740

ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag 4800

gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg 4860

caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg 4920

tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat 4980

gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag 5040

cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt 5100

tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt 5160

tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc 5220

gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag 5280

cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag 5340

cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt 5400

agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta 5460

gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac 5520

cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc 5580

gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc 5640

gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg 5700

ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac 5760

cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg 5820

caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac 5880

attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg 5940

tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt 6000

tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa 6060

ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc 6120

tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct 6180

ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc 6240

cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg 6300

acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 6360

agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc 6420

acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg 6480

agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 6540

aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 6600

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 6660

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 6720

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 6780

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 6840

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 6900

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 6960

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 7020

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 7080

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 7140

tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 7200

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 7260

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 7320

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 7380

attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt 7440

ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc 7500

ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg 7560

ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg 7620

tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa 7680

tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca 7740

tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc 7800

gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc 7860

tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg 7920

gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga 7980

acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg 8040

gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc 8100

ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc 8160

agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc 8220

tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt 8280

tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt 8340

tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa 8400

cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc 8460

cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct 8520

tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga 8580

ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg 8640

tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg 8700

aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa 8760

ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat 8820

atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc 8880

acacattatt atggagaaac tcgagcttgt cgatcgacag atccggtcgg catctactct 8940

atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta 9000

cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc 9060

cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat 9120

tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga 9180

gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca 9240

tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga 9300

acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt 9360

tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca 9420

tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc 9480

agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac 9540

cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga 9600

tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt 9660

cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctaaact 9720

ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat 9780

aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc 9840

ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga 9900

cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt 9960

tcatatctca ttgccccccc ggatctgcga aagctcgaga gagatagatt tgtagagaga 10020

gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggt 10080

cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat 10140

ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg 10200

ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat 10260

cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac 10320

agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct 10380

caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt 10440

cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 10500

ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg 10560

catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct 10620

tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc 10680

ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt 10740

tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc 10800

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 10860

gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 10920

ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 10980

cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcc ccgatctagt 11040

aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct 11100

atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac 11160

gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata 11220

atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa 11280

cgatcgggga aattcgagct ctccactgct cttgtttcct cttccctgga aatgtggatc 11340

atctgcaaca actgctcgat catcccagtt aaggcgtcca ccaaaaatat tccagacgcc 11400

attttggttc tgaaattgag cacacctgtg attcaggacg gcatcaccaa gaaccttcat 11460

accggcttca tgaaaacgtt tcacagcctc cttcaactct tccatggtcc catatctgga 11520

atttagatta tacaaatccc ttggcatgta tccttcaggc gacacagaat cagtaggtgg 11580

tggtgaccag acaatggtga aacccatcga tgacaactcc atcgattgaa aatctcgaaa 11640

cagccgtgtc atagtcaatc attaggtgtt ataggaacaa tcaaaggttt tttcaagtgt 11700

taatcttcat actaatatat acagtgggta ctctttatct actgccgtgg aactgtcata 11760

tttgattatg aaattttagc tctagaaaat atttgatcat caatgtcaag actttatgac 11820

cttgcaaaat acatttccta attgagaaca gggtaaaatt atgaactatg cctctgaacc 11880

ttcatacaca ggcagcacat tttttgttgt aaaattcatc ttaatatcag cggaaagact 11940

ggaccagaga aagaaaaagt taagacaggc atatactctt gatcctctaa aagagatgag 12000

gcggtacaat gatcaaccat gaacattaaa gtgatacgtg gaacatgaga acacaaataa 12060

ttgtcactgg aacataaata caaaaaggtt tgtagtaact taccgctagc agatctgtcg 12120

acggagttgt catcgatggg tttcaccatt gtctggtcac caccacctac tgattctgtg 12180

tcgcctgaag gatacatgcc aagggatttg tataatctaa attccagata tgggaccatg 12240

gaagagttga aggaggctgt gaaacgtttt catgaagccg gtatgaaggt tcttggtgat 12300

gccgtcctga atcacaggtg tgctcaattt cagaaccaaa atggcgtctg gaatattttt 12360

ggtggacgcc ttaactggga tgatcgagca gttgttgcag atgatccaca tttccaggga 12420

agaggaaaca agagcagtgg agctctagag tcgacctgca gaagtaacac caaacaacag 12480

ggtgagcatc gacaaaagaa acagtaccaa gcaaataaat agcgtatgaa ggcagggcta 12540

aaaaaatcca catatagctg ctgcatatgc catcatccaa gtatatcaag atcaaaataa 12600

ttataaaaca tacttgttta ttataataga taggtactca aggttagagc atatgaatag 12660

atgctgcata tgccatcatg tatatgcatc agtaaaaccc acatcaacat gtatacctat 12720

cctagatcga tatttccatc catcttaaac tcgtaactat gaagatgtat gacacacaca 12780

tacagttcca aaattaataa atacaccagg tagtttgaaa cagtattcta ctccgatcta 12840

gaacgaatga acgaccgccc aaccacacca catcatcaca accaagcgaa caaaaagcat 12900

ctctgtatat gcatcagtaa aacccgcatc aacatgtata cctatcctag atcgatattt 12960

ccatccatca tcttcaattc gtaactatga atatgtatgg cacacacata cagatccaaa 13020

attaataaat ccaccaggta gtttgaaaca gaattctact ccgatctaga acgaccgccc 13080

aaccagacca catcatcaca accaagacaa aaaaaagcat gaaaagatga cccgacaaac 13140

aagtgcacgg catatattga aataaaggaa aagggcaaac caaaccctat gcaacgaaac 13200

aaaaaaaatc atgaaatcga tcccgtctgc ggaacggcta gagccatccc aggattcccc 13260

aaagagaaac actggcaagt tagcaatcag aacgtgtctg acgtacaggt cgcatccgtg 13320

tacgaacgct agcagcacgg atctaacaca aacacggatc taacacaaac atgaacagaa 13380

gtagaactac cgggccctaa ccatggaccg gaacgccgat ctagagaagg tagagagggg 13440

ggggggggga ggacgagcgg cgtaccttga agcggaggtg ccgacgggtg gatttggggg 13500

agatctggtt gtgtgtgtgt gcgctccgaa caacacgagg ttggggaaag agggtgtgga 13560

gggggtgtct atttattacg gcgggcgagg aagggaaagc gaaggagcgg tgggaaagga 13620

atcccccgta gctgccggtg ccgtgagagg aggaggaggc cgcctgccgt gccggctcac 13680

gtctgccgct ccgccacgca atttctggat gccgacagcg gagcaagtcc aacggtggag 13740

cggaactctc gagaggggtc cagaggcagc gacagagatg ccgtgccgtc tgcttcgctt 13800

ggcccgacgc gacgctgctg gttcgctggt tggtgtccgt tagactcgtc gacggcgttt 13860

aacaggctgg cattatctac tcgaaacaag aaaaatgttt ccttagtttt tttaatttct 13920

taaagggtat ttgtttaatt tttagtcact ttattttatt ctattttata tctaaattat 13980

taaataaaaa aactaaaata gagttttagt tttcttaatt tagaggctaa aatagaataa 14040

aatagatgta ctaaaaaaat tagtctataa aaaccattaa ccctaaaccc taaatggatg 14100

tactaataaa atggatgaag tattatatag gtgaagctat ttgcaaaaaa aaaggagaac 14160

acatgcacac taaaaagata aaactgtaga gtcctgttgt caaaatactc aattgtcctt 14220

tagaccatgt ctaactgttc atttatatga ttctctaaaa cactgatatt attgtagtac 14280

tatagattat attattcgta gagtaaagtt taaatatatg tataaagata gataaactgc 14340

acttcaaaca agtgtgacaa aaaaaatatg tggtaatttt ttataactta gacatgcaat 14400

gctcattatc tctagagagg ggcacgaccg ggtcacgctg cacaagcttg gcactggccg 14460

tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 14520

cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 14580

aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg 14640

tttcccgcct tcagtttagc ttcatggagt caaagattca aatagaggac ctaacagaac 14700

tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga 14760

aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata 14820

cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc 14880

tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag 14940

gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg 15000

ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg 15060

ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg 15120

acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt 15180

tggagagaac acgggggact cttgac 15206

<210> 7

<211> 15647

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 7

aattaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt 60

ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta 120

tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc 180

tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg 240

cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc 300

gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg 360

aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac 420

ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag 480

atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct 540

ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg 600

gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg 660

gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag 720

ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga 780

ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag 840

ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc 900

tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg 960

cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat 1020

gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca 1080

ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc 1140

acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg 1200

cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg 1260

tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata 1320

aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc 1380

aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt 1440

tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga 1500

tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat 1560

cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc 1620

cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg 1680

ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct 1740

acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc 1800

cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag 1860

ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc 1920

tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga 1980

ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc 2040

agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac 2100

tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag 2160

accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga 2220

ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc 2280

aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag 2340

cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc 2400

gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg 2460

tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc 2520

ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg 2580

cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg 2640

ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt 2700

tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg 2760

ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg 2820

tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag 2880

acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag 2940

ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc 3000

acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg 3060

gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca 3120

tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg 3180

tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc 3240

gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg 3300

aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg 3360

ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc 3420

tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg 3480

agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg 3540

tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg 3600

ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga 3660

aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc 3720

tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc 3780

ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc 3840

gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt 3900

cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac 3960

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 4020

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 4080

gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag 4140

agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 4200

gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4260

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4320

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4380

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4440

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4500

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4560

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4620

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4680

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4740

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4800

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4860

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4920

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4980

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5040

actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa 5100

tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga 5160

catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc 5220

acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca 5280

caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt 5340

ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt 5400

tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc 5460

tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc 5520

actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc 5580

aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt 5640

gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt 5700

ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat 5760

tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc 5820

ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt 5880

tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa 5940

ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa 6000

gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg 6060

atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat 6120

ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc 6180

aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct 6240

gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc 6300

aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg 6360

tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat 6420

tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac 6480

taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta 6540

tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg acagatccgg 6600

tcggcatcta ctctatttct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc 6660

ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt 6720

acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc 6780

tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag 6840

catatacgcc cggagtcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg 6900

cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta 6960

ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc 7020

cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc 7080

ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc 7140

catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca 7200

tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag 7260

atcggccgca gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag cgggcagttc 7320

ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag 7380

gctctcgcta aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa 7440

gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac 7500

atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg 7560

catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt 7620

gagttcaggc tttttcatat ctcattgccc ccccggatct gcgaaagctc gagagagata 7680

gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt 7740

atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga 7800

gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat 7860

gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat 7920

agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct 7980

tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct 8040

ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga 8100

gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt 8160

ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc 8220

actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta 8280

ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa 8340

tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc 8400

tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc 8460

aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 8520

agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 8580

agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 8640

tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa 8700

ttccccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct 8760

atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc 8820

atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca 8880

gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt 8940

gccaaatgtt tgaacgatcg gggaaattcg agcttctaca atagaagttc tgcaagtacc 9000

agcgcggcaa gtctacgatg acacttccca gaccttgtaa tcctgtccct cggcagcaac 9060

tacccagttt gtggggccgc ttggctcaaa atgtcctgcg ccaatcttca ttgttaccct 9120

ctcatcaatc tctgccgcat ataaatttcc ctctgctttc agtatcttga tcttgctacg 9180

gcaatggatc ttttggcgat ttctaataga aattaattta gcaatctctg gctgtaaatg 9240

cgaaaatata tgatcataga agattgcagg agtgcctggg tgagatcgat tgaaaatctc 9300

gaaacagccg tgtcatagtc aatcattagg tgttatagga acaatcaaag gttttttcaa 9360

gtgttaatct tcatactaat atatacagtg ggtactcttt atctactgcc gtggaactgt 9420

catatttgat tatgaaattt tagctctaga aaatatttga tcatcaatgt caagacttta 9480

tgaccttgca aaatacattt cctaattgag aacagggtaa aattatgaac tatgcctctg 9540

aaccttcata cacaggcagc acattttttg ttgtaaaatt catcttaata tcagcggaaa 9600

gactggacca gagaaagaaa aagttaagac aggcatatac tcttgatcct ctaaaagaga 9660

tgaggcggta caatgatcaa ccatgaacat taaagtgata cgtggaacat gagaacacaa 9720

ataattgtca ctggaacata aatacaaaaa ggtttgtagt aacttaccgc tagcagatct 9780

gtcgacctca cccaggcact cctgcaatct tctatgatca tatattttcg catttacagc 9840

cagagattgc taaattaatt tctattagaa atcgccaaaa gatccattgc cgtagcaaga 9900

tcaagatact gaaagcagag ggaaatttat atgcggcaga gattgatgag agggtaacaa 9960

tgaagattgg cgcaggacat tttgagccaa gcggccccac aaactgggta gttgctgccg 10020

agggacagga ttacaaggtc tgggaagtgt catcgtagac ttgccgcgct ggtacttgca 10080

gaacttctat tgtagactct agagtcgacc tgcagaagta acaccaaaca acagggtgag 10140

catcgacaaa agaaacagta ccaagcaaat aaatagcgta tgaaggcagg gctaaaaaaa 10200

tccacatata gctgctgcat atgccatcat ccaagtatat caagatcaaa ataattataa 10260

aacatacttg tttattataa tagataggta ctcaaggtta gagcatatga atagatgctg 10320

catatgccat catgtatatg catcagtaaa acccacatca acatgtatac ctatcctaga 10380

tcgatatttc catccatctt aaactcgtaa ctatgaagat gtatgacaca cacatacagt 10440

tccaaaatta ataaatacac caggtagttt gaaacagtat tctactccga tctagaacga 10500

atgaacgacc gcccaaccac accacatcat cacaaccaag cgaacaaaaa gcatctctgt 10560

atatgcatca gtaaaacccg catcaacatg tatacctatc ctagatcgat atttccatcc 10620

atcatcttca attcgtaact atgaatatgt atggcacaca catacagatc caaaattaat 10680

aaatccacca ggtagtttga aacagaattc tactccgatc tagaacgacc gcccaaccag 10740

accacatcat cacaaccaag acaaaaaaaa gcatgaaaag atgacccgac aaacaagtgc 10800

acggcatata ttgaaataaa ggaaaagggc aaaccaaacc ctatgcaacg aaacaaaaaa 10860

aatcatgaaa tcgatcccgt ctgcggaacg gctagagcca tcccaggatt ccccaaagag 10920

aaacactggc aagttagcaa tcagaacgtg tctgacgtac aggtcgcatc cgtgtacgaa 10980

cgctagcagc acggatctaa cacaaacacg gatctaacac aaacatgaac agaagtagaa 11040

ctaccgggcc ctaaccatgg accggaacgc cgatctagag aaggtagaga gggggggggg 11100

gggaggacga gcggcgtacc ttgaagcgga ggtgccgacg ggtggatttg ggggagatct 11160

ggttgtgtgt gtgtgcgctc cgaacaacac gaggttgggg aaagagggtg tggagggggt 11220

gtctatttat tacggcgggc gaggaaggga aagcgaagga gcggtgggaa aggaatcccc 11280

cgtagctgcc ggtgccgtga gaggaggagg aggccgcctg ccgtgccggc tcacgtctgc 11340

cgctccgcca cgcaatttct ggatgccgac agcggagcaa gtccaacggt ggagcggaac 11400

tctcgagagg ggtccagagg cagcgacaga gatgccgtgc cgtctgcttc gcttggcccg 11460

acgcgacgct gctggttcgc tggttggtgt ccgttagact cgtcgacggc gtttaacagg 11520

ctggcattat ctactcgaaa caagaaaaat gtttccttag tttttttaat ttcttaaagg 11580

gtatttgttt aatttttagt cactttattt tattctattt tatatctaaa ttattaaata 11640

aaaaaactaa aatagagttt tagttttctt aatttagagg ctaaaataga ataaaataga 11700

tgtactaaaa aaattagtct ataaaaacca ttaaccctaa accctaaatg gatgtactaa 11760

taaaatggat gaagtattat ataggtgaag ctatttgcaa aaaaaaagga gaacacatgc 11820

acactaaaaa gataaaactg tagagtcctg ttgtcaaaat actcaattgt cctttagacc 11880

atgtctaact gttcatttat atgattctct aaaacactga tattattgta gtactataga 11940

ttatattatt cgtagagtaa agtttaaata tatgtataaa gatagataaa ctgcacttca 12000

aacaagtgtg acaaaaaaaa tatgtggtaa ttttttataa cttagacatg caatgctcat 12060

tatctctaga gaggggcacg accgggtcac gctgcacaag cttggcactg gccgtcgttt 12120

tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 12180

cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 12240

tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc 12300

gccttcagtt tagcttcttg accccgggcg tattggctag agcagcttgc caacatggtg 12360

gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg 12420

gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca 12480

gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat 12540

cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat 12600

ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag 12660

caagtggatt gatgtgaaca tggtggagca cgacactctc gtctactcca agaatatcaa 12720

agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg 12780

aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa 12840

ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc 12900

ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga 12960

agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag 13020

ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt 13080

tcatttggag aggacacgct gaaatcacca gtctctctct acaaatctat ctcttcgcga 13140

cccgggtaca gggttccttg cgtgaagaag ggtggcctgc ggttcaccat taacggtcac 13200

gactacttcc agctagtact ggtgaccaac gtcgcggcgg cagggtcaat caagtccatg 13260

gaggttatgg gttccaacac agcggattgg atgccgatgg cacgtaactg gggcgcccaa 13320

tggcactcac tggcctacct caccggtcaa ggtctatcct ttagggtcac caacacagat 13380

gaccaaacgc tcgtcttcac caacgtcgtg ccaccaggat ggaagtttgg ccagacattt 13440

gcaagcaagc tgcagttcaa gtgagaggag aagcctgaat tgataccgga gcgtttcttt 13500

tgggagtaac atctctggtt gcctagcaaa catatgattg tatataagtt tcgttgtgcg 13560

tttattcttt cggtgtgtaa aataacatac atgctttcct gatattttct tgtatatatg 13620

tacacacaca cgacaaatcc ttccatttct attattattg aacaatttaa ttgcgagggc 13680

gagtacttgt ctgtttacct tttttttttc agatggcatt ttatagttta acctttcatg 13740

gaccggcagt agttctaacc atgaatgaaa agaaatcata gtccacacca cgcagggaca 13800

ttgtggtcat tttagacaag acgatttgat taatgtcttg tatgatatgg tcgacagtga 13860

ggactaacaa acatatggca tattttatta ccggcgagtt aaataaattt atgtcacagt 13920

aataaactgc ctaataaatg cacgccagaa aatataatga taaaaaaaag aaaagataca 13980

taagtccatt gcttctactt ttttaaaaat taaatccaac attttctatt ttttggtata 14040

aacttggaag tactagttgg atatgcaaaa tcatctaacc tccatatatt tcatcaattt 14100

gtttacttta catatgggag aggatagtat gtcaaagaaa atgacaacaa tcttacaagt 14160

ttcttatttt aaaagttccg ctaacttatc aagcatagtg tgccacgcaa aactgacaac 14220

aaaccaacaa atttaaggag cgcctaactt atcatctatg acataccgca caaaatgata 14280

acatactaga gaaactttat tgcacaaaag gaaatttatc cataaggcaa aggaacatct 14340

taaggctttg gatatacatt taccaacaag cattgtttgt attaccccta aagcgcaaga 14400

catgtcatcc atgagtcata gtgtgtatat ctcaacattg caaagctacc ttttttctat 14460

tatacttttc gcattatagg ctagatatta tctatacatg tcaacaaact ctatccctac 14520

gtcatatctg aagattcttt tcttcactat ataagttggc ttccctgtca ttgaactcac 14580

atcaaccagc ccaagtttcc aataacatcc tcaaatagct atgaagatca ttttcgtatt 14640

tgctctcctt gctattgttg catgcaacgc tagcgctatg gttagcaaag gagaggagct 14700

tttcaccggc gtcgtcccga tactggtgga gttggatgga gatgttaatg gtcataaatt 14760

ttctgtatcg ggtgaaggcg agggcgacgc cacctatggc aaattaacac tgaagttcat 14820

ctgtactact gggaagctcc cggtgccgtg gccgacgctc gtcaccactc ttacctacgg 14880

cgtgcagtgc ttctcacgct acccggatca catgaagcaa catgattttt ttaaatccgc 14940

catgccggag ggatatgttc aagaaagaac catcttcttc aaggatgatg gcaattataa 15000

aacgcgggca gaggtaaagt ttgaaggaga cacattggtc aaccgcattg agctaaaggg 15060

gattgacttc aaagaagatg gtaacatcct cggccacaaa ctggagtaca actacaattc 15120

gcacaatgtg tacatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 15180

ccggcacaac attgaagacg gcagcgttca gttggctgat cactaccagc agaatactcc 15240

aattggtgat gggcctgtgc tcctccccga caaccattat ctatcaacac aaagtgctct 15300

ctccaaggac ccaaatgaaa agcgcgacca catggtgctg cttgagttcg tcacggcggc 15360

ggggataacg ctcggcatgg acgagctgta caagtgaata tgaagatgaa gatgaaatat 15420

ttggtgtgtc aaataaaaag catgtgtgct taagtttgtg tttttttctt ggcttgttgt 15480

gttatgaatt tgtggctttt tctaatatta aatgaatgta agaactcatt ataatgaata 15540

aacaaatgtt tctataatcc attgtgaatg ttttgttgga tctcttctgc agcatataac 15600

tactgtatgt gctatggtat ggactatgga atatgattaa agataag 15647

<210> 8

<211> 48

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 8

atcggggaaa ttcgagcttc tacaatagaa gttctgcaag taccagcg 48

<210> 9

<211> 35

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 9

attttcaatc gatctcaccc aggcactcct gcaat 35

<210> 10

<211> 41

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 10

gcctgggtga gatcgattga aaatctcgaa acagccgtgt c 41

<210> 11

<211> 34

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 11

tgggtgaggt cgacagatct gctagcggta agtt 34

<210> 12

<211> 30

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 12

ctgtcgacct cacccaggca ctcctgcaat 30

<210> 13

<211> 49

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 13

ctgcaggtcg actctagagt ctacaataga agttctgcaa gtaccagcg 49

<210> 14

<211> 24

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 14

tcgcctgttg atgtatgcat gttc 24

<210> 15

<211> 33

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 15

gtgtatcaca tagtattaca tagctccaac atg 33

<210> 16

<211> 21

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 16

agcatgaaga tcaaggtggt c 21

<210> 17

<211> 18

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 17

gccttggcaa tccacatc 18

<210> 18

<211> 15641

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 18

aattaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt 60

ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta 120

tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc 180

tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg 240

cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc 300

gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg 360

aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac 420

ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag 480

atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct 540

ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg 600

gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg 660

gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag 720

ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga 780

ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag 840

ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc 900

tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg 960

cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat 1020

gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca 1080

ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc 1140

acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg 1200

cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg 1260

tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata 1320

aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc 1380

aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt 1440

tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga 1500

tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat 1560

cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc 1620

cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg 1680

ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct 1740

acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc 1800

cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag 1860

ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc 1920

tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga 1980

ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc 2040

agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac 2100

tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag 2160

accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga 2220

ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc 2280

aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag 2340

cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc 2400

gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg 2460

tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc 2520

ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg 2580

cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg 2640

ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt 2700

tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg 2760

ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg 2820

tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag 2880

acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag 2940

ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc 3000

acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg 3060

gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca 3120

tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg 3180

tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc 3240

gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg 3300

aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg 3360

ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc 3420

tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg 3480

agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg 3540

tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg 3600

ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga 3660

aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc 3720

tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc 3780

ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc 3840

gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt 3900

cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac 3960

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 4020

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 4080

gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag 4140

agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 4200

gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4260

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4320

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4380

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4440

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4500

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4560

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4620

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4680

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4740

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4800

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4860

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4920

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4980

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5040

actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa 5100

tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga 5160

catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc 5220

acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca 5280

caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt 5340

ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt 5400

tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc 5460

tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc 5520

actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc 5580

aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt 5640

gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt 5700

ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat 5760

tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc 5820

ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt 5880

tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa 5940

ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa 6000

gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg 6060

atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat 6120

ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc 6180

aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct 6240

gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc 6300

aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg 6360

tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat 6420

tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac 6480

taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta 6540

tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg acagatccgg 6600

tcggcatcta ctctatttct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc 6660

ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt 6720

acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc 6780

tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag 6840

catatacgcc cggagtcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg 6900

cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta 6960

ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc 7020

cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc 7080

ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc 7140

catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca 7200

tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag 7260

atcggccgca gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag cgggcagttc 7320

ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag 7380

gctctcgcta aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa 7440

gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac 7500

atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg 7560

catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt 7620

gagttcaggc tttttcatat ctcattgccc ccccggatct gcgaaagctc gagagagata 7680

gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt 7740

atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga 7800

gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat 7860

gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat 7920

agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct 7980

tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct 8040

ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga 8100

gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt 8160

ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc 8220

actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta 8280

ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa 8340

tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc 8400

tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc 8460

aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 8520

agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 8580

agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 8640

tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa 8700

ttccccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct 8760

atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc 8820

atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca 8880

gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt 8940

gccaaatgtt tgaacgatcg gggaaattcg agcttctaca atagaagttc tgcaagtacc 9000

agcgcggcaa gtctacgatg acacttccca gaccttgtaa tcctgtccct cggcagcaac 9060

tacccagttt gtggggccgc ttggctcaaa atgtcctgcg ccaatcttca ttgttaccct 9120

ctcatcaatc tctgccgcat ataaatttcc ctctgctttc agtatcttga tcttgctacg 9180

gcaatggatc ttttggcgat ttctaataga aattaattta gcaatctctg gctgtaaatg 9240

cgaaaatata tgatcataga agattgcagg agtgcctggg tgagatcgat tgaaaatctc 9300

gaaacagccg tgtcatagtc aatcattagg tgttatagga acaatcaaag gttttttcaa 9360

gtgttaatct tcatactaat atatacagtg ggtactcttt atctactgcc gtggaactgt 9420

catatttgat tatgaaattt tagctctaga aaatatttga tcatcaatgt caagacttta 9480

tgaccttgca aaatacattt cctaattgag aacagggtaa aattatgaac tatgcctctg 9540

aaccttcata cacaggcagc acattttttg ttgtaaaatt catcttaata tcagcggaaa 9600

gactggacca gagaaagaaa aagttaagac aggcatatac tcttgatcct ctaaaagaga 9660

tgaggcggta caatgatcaa ccatgaacat taaagtgata cgtggaacat gagaacacaa 9720

ataattgtca ctggaacata aatacaaaaa ggtttgtagt aacttaccgc tagcagatct 9780

gtcgacctca cccaggcact cctgcaatct tctatgatca tatattttcg catttacagc 9840

cagagattgc taaattaatt tctattagaa atcgccaaaa gatccattgc cgtagcaaga 9900

tcaagatact gaaagcagag ggaaatttat atgcggcaga gattgatgag agggtaacaa 9960

tgaagattgg cgcaggacat tttgagccaa gcggccccac aaactgggta gttgctgccg 10020

agggacagga ttacaaggtc tgggaagtgt catcgtagac ttgccgcgct ggtacttgca 10080

gaacttctat tgtagactct agagtcgacc tgcagaagta acaccaaaca acagggtgag 10140

catcgacaaa agaaacagta ccaagcaaat aaatagcgta tgaaggcagg gctaaaaaaa 10200

tccacatata gctgctgcat atgccatcat ccaagtatat caagatcaaa ataattataa 10260

aacatacttg tttattataa tagataggta ctcaaggtta gagcatatga atagatgctg 10320

catatgccat catgtatatg catcagtaaa acccacatca acatgtatac ctatcctaga 10380

tcgatatttc catccatctt aaactcgtaa ctatgaagat gtatgacaca cacatacagt 10440

tccaaaatta ataaatacac caggtagttt gaaacagtat tctactccga tctagaacga 10500

atgaacgacc gcccaaccac accacatcat cacaaccaag cgaacaaaaa gcatctctgt 10560

atatgcatca gtaaaacccg catcaacatg tatacctatc ctagatcgat atttccatcc 10620

atcatcttca attcgtaact atgaatatgt atggcacaca catacagatc caaaattaat 10680

aaatccacca ggtagtttga aacagaattc tactccgatc tagaacgacc gcccaaccag 10740

accacatcat cacaaccaag acaaaaaaaa gcatgaaaag atgacccgac aaacaagtgc 10800

acggcatata ttgaaataaa ggaaaagggc aaaccaaacc ctatgcaacg aaacaaaaaa 10860

aatcatgaaa tcgatcccgt ctgcggaacg gctagagcca tcccaggatt ccccaaagag 10920

aaacactggc aagttagcaa tcagaacgtg tctgacgtac aggtcgcatc cgtgtacgaa 10980

cgctagcagc acggatctaa cacaaacacg gatctaacac aaacatgaac agaagtagaa 11040

ctaccgggcc ctaaccatgg accggaacgc cgatctagag aaggtagaga gggggggggg 11100

gggaggacga gcggcgtacc ttgaagcgga ggtgccgacg ggtggatttg ggggagatct 11160

ggttgtgtgt gtgtgcgctc cgaacaacac gaggttgggg aaagagggtg tggagggggt 11220

gtctatttat tacggcgggc gaggaaggga aagcgaagga gcggtgggaa aggaatcccc 11280

cgtagctgcc ggtgccgtga gaggaggagg aggccgcctg ccgtgccggc tcacgtctgc 11340

cgctccgcca cgcaatttct ggatgccgac agcggagcaa gtccaacggt ggagcggaac 11400

tctcgagagg ggtccagagg cagcgacaga gatgccgtgc cgtctgcttc gcttggcccg 11460

acgcgacgct gctggttcgc tggttggtgt ccgttagact cgtcgacggc gtttaacagg 11520

ctggcattat ctactcgaaa caagaaaaat gtttccttag tttttttaat ttcttaaagg 11580

gtatttgttt aatttttagt cactttattt tattctattt tatatctaaa ttattaaata 11640

aaaaaactaa aatagagttt tagttttctt aatttagagg ctaaaataga ataaaataga 11700

tgtactaaaa aaattagtct ataaaaacca ttaaccctaa accctaaatg gatgtactaa 11760

taaaatggat gaagtattat ataggtgaag ctatttgcaa aaaaaaagga gaacacatgc 11820

acactaaaaa gataaaactg tagagtcctg ttgtcaaaat actcaattgt cctttagacc 11880

atgtctaact gttcatttat atgattctct aaaacactga tattattgta gtactataga 11940

ttatattatt cgtagagtaa agtttaaata tatgtataaa gatagataaa ctgcacttca 12000

aacaagtgtg acaaaaaaaa tatgtggtaa ttttttataa cttagacatg caatgctcat 12060

tatctctaga gaggggcacg accgggtcac gctgcacaag cttggcactg gccgtcgttt 12120

tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 12180

cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 12240

tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc 12300

gccttcagtt tagcttcccg ggcgtattgg ctagagcagc ttgccaacat ggtggagcac 12360

gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt 12420

gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc 12480

tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc 12540

gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc 12600

ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg 12660

gattgatgtg aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac 12720

agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct 12780

cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg 12840

tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc 12900

cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt 12960

tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga 13020

cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt 13080

ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctcttc gcgacccggg 13140

tacagggttc cttgcgtgaa gaagggtggc ctgcggttca ccattaacgg tcacgactac 13200

ttccagctag tactggtgac caacgtcgcg gcggcagggt caatcaagtc catggaggtt 13260

atgggttcca acacagcgga ttggatgccg atggcacgta actggggcgc ccaatggcac 13320

tcactggcct acctcaccgg tcaaggtcta tcctttaggg tcaccaacac agatgaccaa 13380

acgctcgtct tcaccaacgt cgtgccacca ggatggaagt ttggccagac atttgcaagc 13440

aagctgcagt tcaagtgaga ggagaagcct gaattgatac cggagcgttt cttttgggag 13500

taacatctct ggttgcctag caaacatatg attgtatata agtttcgttg tgcgtttatt 13560

ctttcggtgt gtaaaataac atacatgctt tcctgatatt ttcttgtata tatgtacaca 13620

cacacgacaa atccttccat ttctattatt attgaacaat ttaattgcga gggcgagtac 13680

ttgtctgttt accttttttt tttcagatgg cattttatag tttaaccttt catggaccgg 13740

cagtagttct aaccatgaat gaaaagaaat catagtccac accacgcagg gacattgtgg 13800

tcattttaga caagacgatt tgattaatgt cttgtatgat atggtcgaca gtgaggacta 13860

acaaacatat ggcatatttt attaccggcg agttaaataa atttatgtca cagtaataaa 13920

ctgcctaata aatgcacgcc agaaaatata atgataaaaa aaagaaaaga tacataagtc 13980

cattgcttct acttttttaa aaattaaatc caacattttc tattttttgg tataaacttg 14040

gaagtactag ttggatatgc aaaatcatct aacctccata tatttcatca atttgtttac 14100

tttacatatg ggagaggata gtatgtcaaa gaaaatgaca acaatcttac aagtttctta 14160

ttttaaaagt tccgctaact tatcaagcat agtgtgccac gcaaaactga caacaaacca 14220

acaaatttaa ggagcgccta acttatcatc tatgacatac cgcacaaaat gataacatac 14280

tagagaaact ttattgcaca aaaggaaatt tatccataag gcaaaggaac atcttaaggc 14340

tttggatata catttaccaa caagcattgt ttgtattacc cctaaagcgc aagacatgtc 14400

atccatgagt catagtgtgt atatctcaac attgcaaagc tacctttttt ctattatact 14460

tttcgcatta taggctagat attatctata catgtcaaca aactctatcc ctacgtcata 14520

tctgaagatt cttttcttca ctatataagt tggcttccct gtcattgaac tcacatcaac 14580

cagcccaagt ttccaataac atcctcaaat agctatgaag atcattttcg tatttgctct 14640

ccttgctatt gttgcatgca acgctagcgc tatggttagc aaaggagagg agcttttcac 14700

cggcgtcgtc ccgatactgg tggagttgga tggagatgtt aatggtcata aattttctgt 14760

atcgggtgaa ggcgagggcg acgccaccta tggcaaatta acactgaagt tcatctgtac 14820

tactgggaag ctcccggtgc cgtggccgac gctcgtcacc actcttacct acggcgtgca 14880

gtgcttctca cgctacccgg atcacatgaa gcaacatgat ttttttaaat ccgccatgcc 14940

ggagggatat gttcaagaaa gaaccatctt cttcaaggat gatggcaatt ataaaacgcg 15000

ggcagaggta aagtttgaag gagacacatt ggtcaaccgc attgagctaa aggggattga 15060

cttcaaagaa gatggtaaca tcctcggcca caaactggag tacaactaca attcgcacaa 15120

tgtgtacatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccggca 15180

caacattgaa gacggcagcg ttcagttggc tgatcactac cagcagaata ctccaattgg 15240

tgatgggcct gtgctcctcc ccgacaacca ttatctatca acacaaagtg ctctctccaa 15300

ggacccaaat gaaaagcgcg accacatggt gctgcttgag ttcgtcacgg cggcggggat 15360

aacgctcggc atggacgagc tgtacaagtg aatatgaaga tgaagatgaa atatttggtg 15420

tgtcaaataa aaagcatgtg tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg 15480

aatttgtggc tttttctaat attaaatgaa tgtaagaact cattataatg aataaacaaa 15540

tgtttctata atccattgtg aatgttttgt tggatctctt ctgcagcata taactactgt 15600

atgtgctatg gtatggacta tggaatatga ttaaagataa g 15641

<210> 19

<211> 15521

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 19

aattaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt 60

ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta 120

tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc 180

tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg 240

cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc 300

gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg 360

aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac 420

ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag 480

atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct 540

ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg 600

gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg 660

gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag 720

ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga 780

ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag 840

ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc 900

tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg 960

cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat 1020

gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca 1080

ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc 1140

acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg 1200

cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg 1260

tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata 1320

aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc 1380

aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt 1440

tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga 1500

tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat 1560

cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc 1620

cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg 1680

ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct 1740

acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc 1800

cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag 1860

ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc 1920

tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga 1980

ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc 2040

agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac 2100

tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag 2160

accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga 2220

ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc 2280

aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag 2340

cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc 2400

gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg 2460

tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc 2520

ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg 2580

cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg 2640

ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt 2700

tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg 2760

ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg 2820

tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag 2880

acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag 2940

ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc 3000

acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg 3060

gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca 3120

tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg 3180

tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc 3240

gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg 3300

aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg 3360

ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc 3420

tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg 3480

agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg 3540

tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg 3600

ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga 3660

aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc 3720

tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc 3780

ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc 3840

gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt 3900

cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac 3960

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 4020

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 4080

gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag 4140

agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 4200

gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4260

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4320

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4380

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4440

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4500

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4560

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4620

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4680

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4740

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4800

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4860

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4920

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4980

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5040

actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa 5100

tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga 5160

catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc 5220

acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca 5280

caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt 5340

ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt 5400

tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc 5460

tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc 5520

actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc 5580

aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt 5640

gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt 5700

ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat 5760

tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc 5820

ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt 5880

tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa 5940

ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa 6000

gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg 6060

atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat 6120

ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc 6180

aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct 6240

gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc 6300

aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg 6360

tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat 6420

tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac 6480

taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta 6540

tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg acagatccgg 6600

tcggcatcta ctctatttct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc 6660

ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt 6720

acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc 6780

tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag 6840

catatacgcc cggagtcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg 6900

cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta 6960

ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc 7020

cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc 7080

ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc 7140

catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca 7200

tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag 7260

atcggccgca gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag cgggcagttc 7320

ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag 7380

gctctcgcta aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa 7440

gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac 7500

atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg 7560

catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt 7620

gagttcaggc tttttcatat ctcattgccc ccccggatct gcgaaagctc gagagagata 7680

gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt 7740

atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga 7800

gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat 7860

gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat 7920

agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct 7980

tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct 8040

ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga 8100

gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt 8160

ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc 8220

actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta 8280

ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa 8340

tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc 8400

tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc 8460

aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 8520

agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 8580

agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 8640

tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa 8700

ttccccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct 8760

atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc 8820

atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca 8880

gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt 8940

gccaaatgtt tgaacgatcg gggaaattcg agcttctata aatgtgacag cacgcgaagg 9000

ccaccaacct aacactccag ggggttttcc tttttcatca gacagacgcc agtactcaga 9060

tctttccagt gcagagtgaa gtattccttt cgtggtaaca tcaaatgcac cagcagttcc 9120

atttgtagca tttatccaat caactattct ctgcctgtgg gcatcttgat tataatccat 9180

ttcaccatag gtgtaactga gagaatccca gtactctcct actgatcgat tgaaaatctc 9240

gaaacagccg tgtcatagtc aatcattagg tgttatagga acaatcaaag gttttttcaa 9300

gtgttaatct tcatactaat atatacagtg ggtactcttt atctactgcc gtggaactgt 9360

catatttgat tatgaaattt tagctctaga aaatatttga tcatcaatgt caagacttta 9420

tgaccttgca aaatacattt cctaattgag aacagggtaa aattatgaac tatgcctctg 9480

aaccttcata cacaggcagc acattttttg ttgtaaaatt catcttaata tcagcggaaa 9540

gactggacca gagaaagaaa aagttaagac aggcatatac tcttgatcct ctaaaagaga 9600

tgaggcggta caatgatcaa ccatgaacat taaagtgata cgtggaacat gagaacacaa 9660

ataattgtca ctggaacata aatacaaaaa ggtttgtagt aacttaccgc tagcagatct 9720

gtcgaccagt aggagagtac tgggattctc tcagttacac ctatggtgaa atggattata 9780

atcaagatgc ccacaggcag agaatagttg attggataaa tgctacaaat ggaactgctg 9840

gtgcatttga tgttaccacg aaaggaatac ttcactctgc actggaaaga tctgagtact 9900

ggcgtctgtc tgatgaaaaa ggaaaacccc ctggagtgtt aggttggtgg ccttcgcgtg 9960

ctgtcacatt tatagactct agagtcgacc tgcagaagta acaccaaaca acagggtgag 10020

catcgacaaa agaaacagta ccaagcaaat aaatagcgta tgaaggcagg gctaaaaaaa 10080

tccacatata gctgctgcat atgccatcat ccaagtatat caagatcaaa ataattataa 10140

aacatacttg tttattataa tagataggta ctcaaggtta gagcatatga atagatgctg 10200

catatgccat catgtatatg catcagtaaa acccacatca acatgtatac ctatcctaga 10260

tcgatatttc catccatctt aaactcgtaa ctatgaagat gtatgacaca cacatacagt 10320

tccaaaatta ataaatacac caggtagttt gaaacagtat tctactccga tctagaacga 10380

atgaacgacc gcccaaccac accacatcat cacaaccaag cgaacaaaaa gcatctctgt 10440

atatgcatca gtaaaacccg catcaacatg tatacctatc ctagatcgat atttccatcc 10500

atcatcttca attcgtaact atgaatatgt atggcacaca catacagatc caaaattaat 10560

aaatccacca ggtagtttga aacagaattc tactccgatc tagaacgacc gcccaaccag 10620

accacatcat cacaaccaag acaaaaaaaa gcatgaaaag atgacccgac aaacaagtgc 10680

acggcatata ttgaaataaa ggaaaagggc aaaccaaacc ctatgcaacg aaacaaaaaa 10740

aatcatgaaa tcgatcccgt ctgcggaacg gctagagcca tcccaggatt ccccaaagag 10800

aaacactggc aagttagcaa tcagaacgtg tctgacgtac aggtcgcatc cgtgtacgaa 10860

cgctagcagc acggatctaa cacaaacacg gatctaacac aaacatgaac agaagtagaa 10920

ctaccgggcc ctaaccatgg accggaacgc cgatctagag aaggtagaga gggggggggg 10980

gggaggacga gcggcgtacc ttgaagcgga ggtgccgacg ggtggatttg ggggagatct 11040

ggttgtgtgt gtgtgcgctc cgaacaacac gaggttgggg aaagagggtg tggagggggt 11100

gtctatttat tacggcgggc gaggaaggga aagcgaagga gcggtgggaa aggaatcccc 11160

cgtagctgcc ggtgccgtga gaggaggagg aggccgcctg ccgtgccggc tcacgtctgc 11220

cgctccgcca cgcaatttct ggatgccgac agcggagcaa gtccaacggt ggagcggaac 11280

tctcgagagg ggtccagagg cagcgacaga gatgccgtgc cgtctgcttc gcttggcccg 11340

acgcgacgct gctggttcgc tggttggtgt ccgttagact cgtcgacggc gtttaacagg 11400

ctggcattat ctactcgaaa caagaaaaat gtttccttag tttttttaat ttcttaaagg 11460

gtatttgttt aatttttagt cactttattt tattctattt tatatctaaa ttattaaata 11520

aaaaaactaa aatagagttt tagttttctt aatttagagg ctaaaataga ataaaataga 11580

tgtactaaaa aaattagtct ataaaaacca ttaaccctaa accctaaatg gatgtactaa 11640

taaaatggat gaagtattat ataggtgaag ctatttgcaa aaaaaaagga gaacacatgc 11700

acactaaaaa gataaaactg tagagtcctg ttgtcaaaat actcaattgt cctttagacc 11760

atgtctaact gttcatttat atgattctct aaaacactga tattattgta gtactataga 11820

ttatattatt cgtagagtaa agtttaaata tatgtataaa gatagataaa ctgcacttca 11880

aacaagtgtg acaaaaaaaa tatgtggtaa ttttttataa cttagacatg caatgctcat 11940

tatctctaga gaggggcacg accgggtcac gctgcacaag cttggcactg gccgtcgttt 12000

tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 12060

cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 12120

tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc 12180

gccttcagtt tagcttcccg ggcgtattgg ctagagcagc ttgccaacat ggtggagcac 12240

gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt 12300

gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc 12360

tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc 12420

gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc 12480

ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg 12540

gattgatgtg aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac 12600

agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct 12660

cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg 12720

tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc 12780

cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt 12840

tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga 12900

cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt 12960

ggagaggaca cgctgaaatc accagtctct ctctacaaat ctatctcttc gcgacccggg 13020

tacagggttc cttgcgtgaa gaagggtggc ctgcggttca ccattaacgg tcacgactac 13080

ttccagctag tactggtgac caacgtcgcg gcggcagggt caatcaagtc catggaggtt 13140

atgggttcca acacagcgga ttggatgccg atggcacgta actggggcgc ccaatggcac 13200

tcactggcct acctcaccgg tcaaggtcta tcctttaggg tcaccaacac agatgaccaa 13260

acgctcgtct tcaccaacgt cgtgccacca ggatggaagt ttggccagac atttgcaagc 13320

aagctgcagt tcaagtgaga ggagaagcct gaattgatac cggagcgttt cttttgggag 13380

taacatctct ggttgcctag caaacatatg attgtatata agtttcgttg tgcgtttatt 13440

ctttcggtgt gtaaaataac atacatgctt tcctgatatt ttcttgtata tatgtacaca 13500

cacacgacaa atccttccat ttctattatt attgaacaat ttaattgcga gggcgagtac 13560

ttgtctgttt accttttttt tttcagatgg cattttatag tttaaccttt catggaccgg 13620

cagtagttct aaccatgaat gaaaagaaat catagtccac accacgcagg gacattgtgg 13680

tcattttaga caagacgatt tgattaatgt cttgtatgat atggtcgaca gtgaggacta 13740

acaaacatat ggcatatttt attaccggcg agttaaataa atttatgtca cagtaataaa 13800

ctgcctaata aatgcacgcc agaaaatata atgataaaaa aaagaaaaga tacataagtc 13860

cattgcttct acttttttaa aaattaaatc caacattttc tattttttgg tataaacttg 13920

gaagtactag ttggatatgc aaaatcatct aacctccata tatttcatca atttgtttac 13980

tttacatatg ggagaggata gtatgtcaaa gaaaatgaca acaatcttac aagtttctta 14040

ttttaaaagt tccgctaact tatcaagcat agtgtgccac gcaaaactga caacaaacca 14100

acaaatttaa ggagcgccta acttatcatc tatgacatac cgcacaaaat gataacatac 14160

tagagaaact ttattgcaca aaaggaaatt tatccataag gcaaaggaac atcttaaggc 14220

tttggatata catttaccaa caagcattgt ttgtattacc cctaaagcgc aagacatgtc 14280

atccatgagt catagtgtgt atatctcaac attgcaaagc tacctttttt ctattatact 14340

tttcgcatta taggctagat attatctata catgtcaaca aactctatcc ctacgtcata 14400

tctgaagatt cttttcttca ctatataagt tggcttccct gtcattgaac tcacatcaac 14460

cagcccaagt ttccaataac atcctcaaat agctatgaag atcattttcg tatttgctct 14520

ccttgctatt gttgcatgca acgctagcgc tatggttagc aaaggagagg agcttttcac 14580

cggcgtcgtc ccgatactgg tggagttgga tggagatgtt aatggtcata aattttctgt 14640

atcgggtgaa ggcgagggcg acgccaccta tggcaaatta acactgaagt tcatctgtac 14700

tactgggaag ctcccggtgc cgtggccgac gctcgtcacc actcttacct acggcgtgca 14760

gtgcttctca cgctacccgg atcacatgaa gcaacatgat ttttttaaat ccgccatgcc 14820

ggagggatat gttcaagaaa gaaccatctt cttcaaggat gatggcaatt ataaaacgcg 14880

ggcagaggta aagtttgaag gagacacatt ggtcaaccgc attgagctaa aggggattga 14940

cttcaaagaa gatggtaaca tcctcggcca caaactggag tacaactaca attcgcacaa 15000

tgtgtacatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccggca 15060

caacattgaa gacggcagcg ttcagttggc tgatcactac cagcagaata ctccaattgg 15120

tgatgggcct gtgctcctcc ccgacaacca ttatctatca acacaaagtg ctctctccaa 15180

ggacccaaat gaaaagcgcg accacatggt gctgcttgag ttcgtcacgg cggcggggat 15240

aacgctcggc atggacgagc tgtacaagtg aatatgaaga tgaagatgaa atatttggtg 15300

tgtcaaataa aaagcatgtg tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg 15360

aatttgtggc tttttctaat attaaatgaa tgtaagaact cattataatg aataaacaaa 15420

tgtttctata atccattgtg aatgttttgt tggatctctt ctgcagcata taactactgt 15480

atgtgctatg gtatggacta tggaatatga ttaaagataa g 15521

<210> 20

<211> 15661

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 20

aattaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt 60

ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta 120

tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc 180

tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg 240

cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc 300

gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg 360

aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac 420

ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag 480

atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct 540

ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg 600

gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg 660

gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag 720

ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga 780

ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag 840

ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc 900

tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg 960

cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat 1020

gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca 1080

ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc 1140

acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg 1200

cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg 1260

tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata 1320

aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc 1380

aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt 1440

tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga 1500

tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat 1560

cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc 1620

cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg 1680

ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct 1740

acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc 1800

cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag 1860

ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc 1920

tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga 1980

ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc 2040

agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac 2100

tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag 2160

accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga 2220

ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc 2280

aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag 2340

cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc 2400

gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg 2460

tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc 2520

ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg 2580

cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg 2640

ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt 2700

tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg 2760

ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg 2820

tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag 2880

acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag 2940

ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc 3000

acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg 3060

gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca 3120

tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg 3180

tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc 3240

gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg 3300

aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg 3360

ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc 3420

tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg 3480

agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg 3540

tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg 3600

ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga 3660

aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc 3720

tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc 3780

ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc 3840

gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt 3900

cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac 3960

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 4020

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 4080

gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag 4140

agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 4200

gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4260

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4320

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4380

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4440

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4500

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4560

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4620

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4680

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4740

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4800

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4860

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4920

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4980

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5040

actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa 5100

tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga 5160

catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc 5220

acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca 5280

caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt 5340

ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt 5400

tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc 5460

tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc 5520

actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc 5580

aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt 5640

gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt 5700

ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat 5760

tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc 5820

ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt 5880

tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa 5940

ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa 6000

gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg 6060

atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat 6120

ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc 6180

aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct 6240

gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc 6300

aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg 6360

tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat 6420

tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac 6480

taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta 6540

tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg acagatccgg 6600

tcggcatcta ctctatttct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc 6660

ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt 6720

acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc 6780

tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag 6840

catatacgcc cggagtcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg 6900

cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta 6960

ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc 7020

cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc 7080

ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc 7140

catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca 7200

tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag 7260

atcggccgca gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag cgggcagttc 7320

ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag 7380

gctctcgcta aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa 7440

gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac 7500

atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg 7560

catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt 7620

gagttcaggc tttttcatat ctcattgccc ccccggatct gcgaaagctc gagagagata 7680

gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt 7740

atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga 7800

gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat 7860

gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat 7920

agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct 7980

tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct 8040

ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga 8100

gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt 8160

ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc 8220

actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta 8280

ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa 8340

tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc 8400

tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc 8460

aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 8520

agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 8580

agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 8640

tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa 8700

ttccccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct 8760

atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc 8820

atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca 8880

gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt 8940

gccaaatgtt tgaacgatcg gggaaattcg agctctccac tgctcttgtt tcctcttccc 9000

tggaaatgtg gatcatctgc aacaactgct cgatcatccc agttaaggcg tccaccaaaa 9060

atattccaga cgccattttg gttctgaaat tgagcacacc tgtgattcag gacggcatca 9120

ccaagaacct tcataccggc ttcatgaaaa cgtttcacag cctccttcaa ctcttccatg 9180

gtcccatatc tggaatttag attatacaaa tcccttggca tgtatccttc aggcgacaca 9240

gaatcagtag gtggtggtga ccagacaatg gtgaaaccca tcgatgacaa ctccatcgat 9300

tgaaaatctc gaaacagccg tgtcatagtc aatcattagg tgttatagga acaatcaaag 9360

gttttttcaa gtgttaatct tcatactaat atatacagtg ggtactcttt atctactgcc 9420

gtggaactgt catatttgat tatgaaattt tagctctaga aaatatttga tcatcaatgt 9480

caagacttta tgaccttgca aaatacattt cctaattgag aacagggtaa aattatgaac 9540

tatgcctctg aaccttcata cacaggcagc acattttttg ttgtaaaatt catcttaata 9600

tcagcggaaa gactggacca gagaaagaaa aagttaagac aggcatatac tcttgatcct 9660

ctaaaagaga tgaggcggta caatgatcaa ccatgaacat taaagtgata cgtggaacat 9720

gagaacacaa ataattgtca ctggaacata aatacaaaaa ggtttgtagt aacttaccgc 9780

tagcagatct gtcgacggag ttgtcatcga tgggtttcac cattgtctgg tcaccaccac 9840

ctactgattc tgtgtcgcct gaaggataca tgccaaggga tttgtataat ctaaattcca 9900

gatatgggac catggaagag ttgaaggagg ctgtgaaacg ttttcatgaa gccggtatga 9960

aggttcttgg tgatgccgtc ctgaatcaca ggtgtgctca atttcagaac caaaatggcg 10020

tctggaatat ttttggtgga cgccttaact gggatgatcg agcagttgtt gcagatgatc 10080

cacatttcca gggaagagga aacaagagca gtggagctct agagtcgacc tgcagaagta 10140

acaccaaaca acagggtgag catcgacaaa agaaacagta ccaagcaaat aaatagcgta 10200

tgaaggcagg gctaaaaaaa tccacatata gctgctgcat atgccatcat ccaagtatat 10260

caagatcaaa ataattataa aacatacttg tttattataa tagataggta ctcaaggtta 10320

gagcatatga atagatgctg catatgccat catgtatatg catcagtaaa acccacatca 10380

acatgtatac ctatcctaga tcgatatttc catccatctt aaactcgtaa ctatgaagat 10440

gtatgacaca cacatacagt tccaaaatta ataaatacac caggtagttt gaaacagtat 10500

tctactccga tctagaacga atgaacgacc gcccaaccac accacatcat cacaaccaag 10560

cgaacaaaaa gcatctctgt atatgcatca gtaaaacccg catcaacatg tatacctatc 10620

ctagatcgat atttccatcc atcatcttca attcgtaact atgaatatgt atggcacaca 10680

catacagatc caaaattaat aaatccacca ggtagtttga aacagaattc tactccgatc 10740

tagaacgacc gcccaaccag accacatcat cacaaccaag acaaaaaaaa gcatgaaaag 10800

atgacccgac aaacaagtgc acggcatata ttgaaataaa ggaaaagggc aaaccaaacc 10860

ctatgcaacg aaacaaaaaa aatcatgaaa tcgatcccgt ctgcggaacg gctagagcca 10920

tcccaggatt ccccaaagag aaacactggc aagttagcaa tcagaacgtg tctgacgtac 10980

aggtcgcatc cgtgtacgaa cgctagcagc acggatctaa cacaaacacg gatctaacac 11040

aaacatgaac agaagtagaa ctaccgggcc ctaaccatgg accggaacgc cgatctagag 11100

aaggtagaga gggggggggg gggaggacga gcggcgtacc ttgaagcgga ggtgccgacg 11160

ggtggatttg ggggagatct ggttgtgtgt gtgtgcgctc cgaacaacac gaggttgggg 11220

aaagagggtg tggagggggt gtctatttat tacggcgggc gaggaaggga aagcgaagga 11280

gcggtgggaa aggaatcccc cgtagctgcc ggtgccgtga gaggaggagg aggccgcctg 11340

ccgtgccggc tcacgtctgc cgctccgcca cgcaatttct ggatgccgac agcggagcaa 11400

gtccaacggt ggagcggaac tctcgagagg ggtccagagg cagcgacaga gatgccgtgc 11460

cgtctgcttc gcttggcccg acgcgacgct gctggttcgc tggttggtgt ccgttagact 11520

cgtcgacggc gtttaacagg ctggcattat ctactcgaaa caagaaaaat gtttccttag 11580

tttttttaat ttcttaaagg gtatttgttt aatttttagt cactttattt tattctattt 11640

tatatctaaa ttattaaata aaaaaactaa aatagagttt tagttttctt aatttagagg 11700

ctaaaataga ataaaataga tgtactaaaa aaattagtct ataaaaacca ttaaccctaa 11760

accctaaatg gatgtactaa taaaatggat gaagtattat ataggtgaag ctatttgcaa 11820

aaaaaaagga gaacacatgc acactaaaaa gataaaactg tagagtcctg ttgtcaaaat 11880

actcaattgt cctttagacc atgtctaact gttcatttat atgattctct aaaacactga 11940

tattattgta gtactataga ttatattatt cgtagagtaa agtttaaata tatgtataaa 12000

gatagataaa ctgcacttca aacaagtgtg acaaaaaaaa tatgtggtaa ttttttataa 12060

cttagacatg caatgctcat tatctctaga gaggggcacg accgggtcac gctgcacaag 12120

cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 12180

taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 12240

cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt 12300

ggatcagatt gtcgtttccc gccttcagtt tagcttcccg ggcgtattgg ctagagcagc 12360

ttgccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct 12420

cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg 12480

gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca 12540

cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca 12600

gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa 12660

ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac 12720

tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 12780

agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 12840

aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 12900

atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 12960

atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc 13020

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata 13080

taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat 13140

ctatctcttc gcgacccggg tacagggttc cttgcgtgaa gaagggtggc ctgcggttca 13200

ccattaacgg tcacgactac ttccagctag tactggtgac caacgtcgcg gcggcagggt 13260

caatcaagtc catggaggtt atgggttcca acacagcgga ttggatgccg atggcacgta 13320

actggggcgc ccaatggcac tcactggcct acctcaccgg tcaaggtcta tcctttaggg 13380

tcaccaacac agatgaccaa acgctcgtct tcaccaacgt cgtgccacca ggatggaagt 13440

ttggccagac atttgcaagc aagctgcagt tcaagtgaga ggagaagcct gaattgatac 13500

cggagcgttt cttttgggag taacatctct ggttgcctag caaacatatg attgtatata 13560

agtttcgttg tgcgtttatt ctttcggtgt gtaaaataac atacatgctt tcctgatatt 13620

ttcttgtata tatgtacaca cacacgacaa atccttccat ttctattatt attgaacaat 13680

ttaattgcga gggcgagtac ttgtctgttt accttttttt tttcagatgg cattttatag 13740

tttaaccttt catggaccgg cagtagttct aaccatgaat gaaaagaaat catagtccac 13800

accacgcagg gacattgtgg tcattttaga caagacgatt tgattaatgt cttgtatgat 13860

atggtcgaca gtgaggacta acaaacatat ggcatatttt attaccggcg agttaaataa 13920

atttatgtca cagtaataaa ctgcctaata aatgcacgcc agaaaatata atgataaaaa 13980

aaagaaaaga tacataagtc cattgcttct acttttttaa aaattaaatc caacattttc 14040

tattttttgg tataaacttg gaagtactag ttggatatgc aaaatcatct aacctccata 14100

tatttcatca atttgtttac tttacatatg ggagaggata gtatgtcaaa gaaaatgaca 14160

acaatcttac aagtttctta ttttaaaagt tccgctaact tatcaagcat agtgtgccac 14220

gcaaaactga caacaaacca acaaatttaa ggagcgccta acttatcatc tatgacatac 14280

cgcacaaaat gataacatac tagagaaact ttattgcaca aaaggaaatt tatccataag 14340

gcaaaggaac atcttaaggc tttggatata catttaccaa caagcattgt ttgtattacc 14400

cctaaagcgc aagacatgtc atccatgagt catagtgtgt atatctcaac attgcaaagc 14460

tacctttttt ctattatact tttcgcatta taggctagat attatctata catgtcaaca 14520

aactctatcc ctacgtcata tctgaagatt cttttcttca ctatataagt tggcttccct 14580

gtcattgaac tcacatcaac cagcccaagt ttccaataac atcctcaaat agctatgaag 14640

atcattttcg tatttgctct ccttgctatt gttgcatgca acgctagcgc tatggttagc 14700

aaaggagagg agcttttcac cggcgtcgtc ccgatactgg tggagttgga tggagatgtt 14760

aatggtcata aattttctgt atcgggtgaa ggcgagggcg acgccaccta tggcaaatta 14820

acactgaagt tcatctgtac tactgggaag ctcccggtgc cgtggccgac gctcgtcacc 14880

actcttacct acggcgtgca gtgcttctca cgctacccgg atcacatgaa gcaacatgat 14940

ttttttaaat ccgccatgcc ggagggatat gttcaagaaa gaaccatctt cttcaaggat 15000

gatggcaatt ataaaacgcg ggcagaggta aagtttgaag gagacacatt ggtcaaccgc 15060

attgagctaa aggggattga cttcaaagaa gatggtaaca tcctcggcca caaactggag 15120

tacaactaca attcgcacaa tgtgtacatc atggccgaca agcagaagaa cggcatcaag 15180

gtgaacttca agatccggca caacattgaa gacggcagcg ttcagttggc tgatcactac 15240

cagcagaata ctccaattgg tgatgggcct gtgctcctcc ccgacaacca ttatctatca 15300

acacaaagtg ctctctccaa ggacccaaat gaaaagcgcg accacatggt gctgcttgag 15360

ttcgtcacgg cggcggggat aacgctcggc atggacgagc tgtacaagtg aatatgaaga 15420

tgaagatgaa atatttggtg tgtcaaataa aaagcatgtg tgcttaagtt tgtgtttttt 15480

tcttggcttg ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagaact 15540

cattataatg aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt 15600

ctgcagcata taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa 15660

g 15661

<210> 21

<211> 14566

<212> DNA

<213> Artificial sequence (Artificial Sequence)

<400> 21

catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct 60

tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga 120

ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt 180

gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaacgtgaaa 240

tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc 300

agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta 360

acgatcagtt cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg 420

aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca 480

ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata 540

cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg 600

tttgtgtgaa caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg 660

aaaacggcaa gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc 720

gcagcgtaat gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc 780

atgtcgcgca agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg 840

tcagcgttga actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg 900

ggactttgca agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac 960

tgtgcgtcac agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc 1020

ggtcagtggc agtgaagggc gaacagttcc tgattaacca caaaccgttc tactttactg 1080

gctttggtcg tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg 1140

tgcacgacca cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc 1200

cttacgctga agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa 1260

ctgctgctgt cggctttaac ctctctttag gcattggttt cgaagcgggc aacaagccga 1320

aagaactgta cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga 1380

ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca 1440

acgaaccgga tacccgtccg caagtgcacg ggaatatttc gccactggcg gaagcaacgc 1500

gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca 1560

ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg 1620

tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc 1680

aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc 1740

tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt 1800

atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg 1860

attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc 1920

gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact 1980

tcggtgaaaa accgcagcag ggaggcaaac aagctagcca ccaccaccac caccacgtgt 2040

gaattacagg tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc 2100

ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac 2160

gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg 2220

attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac 2280

taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag 2340

tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg 2400

gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc 2460

acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt 2520

cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt 2580

tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg 2640

cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc 2700

cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg 2760

ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag 2820

gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac 2880

agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg 2940

catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac 3000

gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct 3060

aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg 3120

cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga 3180

aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg 3240

cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg 3300

tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga 3360

acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc 3420

gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg 3480

cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc 3540

ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac 3600

agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag 3660

gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca 3720

tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt 3780

ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg 3840

ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact 3900

tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag 3960

ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc 4020

tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg 4080

tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg 4140

ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg 4200

ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc 4260

aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat 4320

gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc 4380

aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc 4440

ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct 4500

gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat 4560

gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg 4620

tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct 4680

gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa 4740

ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag 4800

gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg 4860

caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg 4920

tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat 4980

gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag 5040

cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt 5100

tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt 5160

tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc 5220

gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag 5280

cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag 5340

cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt 5400

agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta 5460

gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac 5520

cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc 5580

gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc 5640

gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg 5700

ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac 5760

cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg 5820

caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac 5880

attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg 5940

tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt 6000

tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa 6060

ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc 6120

tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct 6180

ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc 6240

cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg 6300

acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 6360

agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc 6420

acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg 6480

agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 6540

aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 6600

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 6660

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 6720

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 6780

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 6840

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 6900

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 6960

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 7020

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 7080

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 7140

tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 7200

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 7260

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 7320

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 7380

attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt 7440

ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc 7500

ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg 7560

ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg 7620

tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa 7680

tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca 7740

tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc 7800

gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc 7860

tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg 7920

gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga 7980

acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg 8040

gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc 8100

ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc 8160

agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc 8220

tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt 8280

tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt 8340

tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa 8400

cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc 8460

cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct 8520

tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga 8580

ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg 8640

tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg 8700

aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa 8760

ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat 8820

atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc 8880

acacattatt atggagaaac tcgagcttgt cgatcgacag atccggtcgg catctactct 8940

atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta 9000

cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc 9060

cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat 9120

tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga 9180

gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca 9240

tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga 9300

acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt 9360

tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca 9420

tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc 9480

agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac 9540

cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga 9600

tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt 9660

cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctaaact 9720

ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat 9780

aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc 9840

ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga 9900

cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt 9960

tcatatctca ttgccccccc ggatctgcga aagctcgaga gagatagatt tgtagagaga 10020

gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggt 10080

cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat 10140

ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg 10200

ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat 10260

cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac 10320

agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct 10380

caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt 10440

cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 10500

ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg 10560

catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct 10620

tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc 10680

ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt 10740

tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc 10800

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 10860

gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 10920

ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 10980

cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcc ccgatctagt 11040

aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct 11100

atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac 11160

gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata 11220

atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa 11280

cgatcgggga aattcgagct tctacaatag aagttctgca agtaccagcg cggcaagtct 11340

acgatgacac ttcccagacc ttgtaatcct gtccctcggc agcaactacc cagtttgtgg 11400

ggccgcttgg ctcaaaatgt cctgcgccaa tcttcattgt taccctctca tcaatctctg 11460

ccgcatataa atttccctct gctttcagta gtgagttcat cttctgctgt tttacctttc 11520

tgatatctga attctctcat tggtgcgtaa tttttttttt ttggctgtca tcggtatagc 11580

tttcttaagc actcagtagc cttgcaatta tatactgaaa gcagagggaa atttatatgc 11640

ggcagagatt gatgagaggg taacaatgaa gattggcgca ggacattttg agccaagcgg 11700

ccccacaaac tgggtagttg ctgccgaggg acaggattac aaggtctggg aagtgtcatc 11760

gtagacttgc cgcgctggta cttgcagaac ttctattgta gactctagag tcgacctgca 11820

gaagtaacac caaacaacag ggtgagcatc gacaaaagaa acagtaccaa gcaaataaat 11880

agcgtatgaa ggcagggcta aaaaaatcca catatagctg ctgcatatgc catcatccaa 11940

gtatatcaag atcaaaataa ttataaaaca tacttgttta ttataataga taggtactca 12000

aggttagagc atatgaatag atgctgcata tgccatcatg tatatgcatc agtaaaaccc 12060

acatcaacat gtatacctat cctagatcga tatttccatc catcttaaac tcgtaactat 12120

gaagatgtat gacacacaca tacagttcca aaattaataa atacaccagg tagtttgaaa 12180

cagtattcta ctccgatcta gaacgaatga acgaccgccc aaccacacca catcatcaca 12240

accaagcgaa caaaaagcat ctctgtatat gcatcagtaa aacccgcatc aacatgtata 12300

cctatcctag atcgatattt ccatccatca tcttcaattc gtaactatga atatgtatgg 12360

cacacacata cagatccaaa attaataaat ccaccaggta gtttgaaaca gaattctact 12420

ccgatctaga acgaccgccc aaccagacca catcatcaca accaagacaa aaaaaagcat 12480

gaaaagatga cccgacaaac aagtgcacgg catatattga aataaaggaa aagggcaaac 12540

caaaccctat gcaacgaaac aaaaaaaatc atgaaatcga tcccgtctgc ggaacggcta 12600

gagccatccc aggattcccc aaagagaaac actggcaagt tagcaatcag aacgtgtctg 12660

acgtacaggt cgcatccgtg tacgaacgct agcagcacgg atctaacaca aacacggatc 12720

taacacaaac atgaacagaa gtagaactac cgggccctaa ccatggaccg gaacgccgat 12780

ctagagaagg tagagagggg ggggggggga ggacgagcgg cgtaccttga agcggaggtg 12840

ccgacgggtg gatttggggg agatctggtt gtgtgtgtgt gcgctccgaa caacacgagg 12900

ttggggaaag agggtgtgga gggggtgtct atttattacg gcgggcgagg aagggaaagc 12960

gaaggagcgg tgggaaagga atcccccgta gctgccggtg ccgtgagagg aggaggaggc 13020

cgcctgccgt gccggctcac gtctgccgct ccgccacgca atttctggat gccgacagcg 13080

gagcaagtcc aacggtggag cggaactctc gagaggggtc cagaggcagc gacagagatg 13140

ccgtgccgtc tgcttcgctt ggcccgacgc gacgctgctg gttcgctggt tggtgtccgt 13200

tagactcgtc gacggcgttt aacaggctgg cattatctac tcgaaacaag aaaaatgttt 13260

ccttagtttt tttaatttct taaagggtat ttgtttaatt tttagtcact ttattttatt 13320

ctattttata tctaaattat taaataaaaa aactaaaata gagttttagt tttcttaatt 13380

tagaggctaa aatagaataa aatagatgta ctaaaaaaat tagtctataa aaaccattaa 13440

ccctaaaccc taaatggatg tactaataaa atggatgaag tattatatag gtgaagctat 13500

ttgcaaaaaa aaaggagaac acatgcacac taaaaagata aaactgtaga gtcctgttgt 13560

caaaatactc aattgtcctt tagaccatgt ctaactgttc atttatatga ttctctaaaa 13620

cactgatatt attgtagtac tatagattat attattcgta gagtaaagtt taaatatatg 13680

tataaagata gataaactgc acttcaaaca agtgtgacaa aaaaaatatg tggtaatttt 13740

ttataactta gacatgcaat gctcattatc tctagagagg ggcacgaccg ggtcacgctg 13800

cacaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 13860

ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 13920

ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt 13980

gagcttggat cagattgtcg tttcccgcct tcagtttagc ttcatggagt caaagattca 14040

aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt 14100

acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta 14160

ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca 14220

aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt 14280

gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc 14340

catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag 14400

catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat 14460

ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat 14520

ataaggaagt tcatttcatt tggagagaac acgggggact cttgac 14566

Claims (10)

1. A transformation system for a nuclear male sterile maintainer line, the transformation system comprising: a gene silencing element of male gamete abortive element/lethal element, a silencing tag reporting element and a selectable tag element; the gene silencing element is used to inhibit or partially inhibit the function of the male gamete abortive element/lethal element.

2. The transfer system of claim 1, wherein the method of inhibiting or partially inhibiting male gamete abortion/lethal element function using a gene silencing element comprises:

1) Inhibiting or reducing transcription of male gamete abortive/lethal elements, including using RNAi, amiRNA, siRNA, asRNA or Crispri, promoter competitive binding;

2) Inhibiting or reducing protein translated from male gamete abortive/lethal elements;

3) The biochemical function of the product is inhibited by binding, chemical modification, or specific degradation to the product of the male-sterile element/lethal element expression cassette.

3. A method for transferring a nuclear male sterile maintainer line, comprising:

(1) Transferring the transfer system of claim 1 into material a to obtain a first plant;

(2) Hybridizing the first plant obtained in the step (1) serving as a male parent and the nuclear male sterile maintainer line material B serving as a female parent to obtain a second plant; the second plant comprises F1 generation and F1 generation selfing or filial generation materials still containing nuclear male sterile maintainer line sites and transfer system sites;

(3) And pollination the second plant as a male parent into other plant materials to obtain a nuclear male sterile maintainer line which is different from the nuclear male sterile maintainer line material B cytoplasm.

4. The method of claim 3, wherein the method comprises the steps of,

the first plant contains a gene silencing element for inhibiting or partially inhibiting the function of a male gamete abortive element/lethal element;

preferably, the gene silencing element comprises inhibiting the function of a male gamete abortive element/lethal element in a nuclear male sterile maintainer line using RNAi, amiRNA, siRNA, asRNA or Crispri;

more preferably, the gene silencing element consists of a promoter-stem loop structure-terminator, or of a promoter-antisense coding sequence-terminator, or of a target binding sequence of a promoter-dCas 9-terminator, male gamete abortive element/lethal element.

5. The method of claim 4, wherein the method comprises the steps of,

the first plant contains a silent marker reporting element; the silencing mark reporting element is different from a color selection mark gene in a nuclear male sterile maintainer line and is used for distinguishing seeds with different functions;

preferably, the silencing tag reporter element is GUS, GFP, YFP, BFP, CFP, RFP or a tag that distinguishes gene silencing materials by seed color or shape.

6. The method of claim 3 to 5, wherein,

When the male gamete abortive element/lethal element is rice alpha-amylase gene expression cassette, the gene silencing element is a hairpin structure expression cassette containing a hairpin structure formed by DNA fragments shown in SEQ ID No. 1-3.

7. A method for improving cytoplasmic genetic diversity of a nuclear male sterile maintainer line is characterized in that a first plant is used as a male parent to be hybridized with an initial nuclear male sterile maintainer line material to obtain a second plant, and seeds of the second plant are subjected to selfing, backcrossing or hybridization with other materials to obtain a target plant;

the first plant carries a transfer system site i;

the transformation system site i comprises: a gene silencing expression cassette for inhibiting male gamete abortive elements/lethal elements in the nuclear male sterility maintainer line site G, a marker reporting element expression cassette for the transformation system site i, a selectable marker element expression cassette for the transformation system site i;

the nuclear male sterile maintainer line locus G includes, but is not limited to, a male sterile restorer element expression cassette, a male gamete abortive element/lethal element expression cassette, and a maintainer selectable marker expression cassette.

8. The method of claim 7, wherein the step of determining the position of the probe is performed,

the genotype of the first plant is homozygosity ii or heterozygosity i-; preferably, the genotype of the first plant is ii;

The second plant genotype is homozygous GG/ii or heterozygous G-/i-or semi-heterozygous GG/i-, G-/ii, which may carry a nuclear male sterility gene ms, preferably the genotype of the second plant is msms/GG/ii or msms/G-/ii.

9. The method of claim 7, wherein the target plant is a new nuclear male sterile maintainer line obtained by breeding, and the cytoplasm of the target plant is different from that of the initial nuclear male sterile maintainer line; preferably, the second plant is taken as a male parent, the female parent selects a nuclear male sterile maintainer line with different cytoplasm from the initial nuclear male sterile maintainer line, the sterile line or other materials are hybridized, the intermediate materials are obtained through selfing back cross, and finally a new nuclear male sterile maintainer line is obtained, namely the target plant.

10. A plant expression vector GATi comprising a hairpin structure formed by the DNA fragments shown in SEQ ID nos. 1-3 or a nucleotide sequence thereof shown in SEQ ID nos. 4-6 for inhibiting the function of a rice alpha-amylase gene in the nuclear male sterility maintainer locus G according to any one of claims 7-9.