CN116926085B - Passiflora edulis PeERF-2 gene and application thereof - Google Patents
Passiflora edulis PeERF-2 gene and application thereof Download PDFInfo
- Publication number
- CN116926085B CN116926085B CN202311188585.XA CN202311188585A CN116926085B CN 116926085 B CN116926085 B CN 116926085B CN 202311188585 A CN202311188585 A CN 202311188585A CN 116926085 B CN116926085 B CN 116926085B
- Authority
- CN
- China
- Prior art keywords
- gene
- peerf
- plants
- passion flower
- vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000000370 Passiflora edulis Nutrition 0.000 title claims abstract description 62
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 59
- 244000288157 Passiflora edulis Species 0.000 title claims description 58
- 241000196324 Embryophyta Species 0.000 claims abstract description 66
- 235000011925 Passiflora alata Nutrition 0.000 claims abstract description 42
- 235000011922 Passiflora incarnata Nutrition 0.000 claims abstract description 42
- 235000013750 Passiflora mixta Nutrition 0.000 claims abstract description 42
- 235000013731 Passiflora van volxemii Nutrition 0.000 claims abstract description 42
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 15
- 210000004209 hair Anatomy 0.000 claims abstract description 6
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims abstract description 5
- 239000002773 nucleotide Substances 0.000 claims abstract description 3
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 3
- 239000013598 vector Substances 0.000 claims description 35
- 230000014509 gene expression Effects 0.000 claims description 14
- 239000013604 expression vector Substances 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 8
- 108091026890 Coding region Proteins 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 241000219195 Arabidopsis thaliana Species 0.000 claims description 5
- 108091008146 restriction endonucleases Proteins 0.000 claims description 4
- 230000001737 promoting effect Effects 0.000 claims description 3
- 238000011160 research Methods 0.000 abstract description 7
- 238000010367 cloning Methods 0.000 abstract description 5
- 230000001105 regulatory effect Effects 0.000 abstract description 4
- 230000001276 controlling effect Effects 0.000 abstract description 2
- 230000008635 plant growth Effects 0.000 abstract description 2
- 238000013518 transcription Methods 0.000 abstract description 2
- 230000035897 transcription Effects 0.000 abstract description 2
- 241000218996 Passiflora Species 0.000 abstract 4
- 230000009261 transgenic effect Effects 0.000 description 33
- 230000035882 stress Effects 0.000 description 28
- 241000219194 Arabidopsis Species 0.000 description 13
- 238000011282 treatment Methods 0.000 description 13
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 12
- 230000012010 growth Effects 0.000 description 10
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Chemical compound OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 8
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 230000010220 ion permeability Effects 0.000 description 7
- 229940118019 malondialdehyde Drugs 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 6
- 238000011529 RT qPCR Methods 0.000 description 6
- 230000008645 cold stress Effects 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 5
- 230000036579 abiotic stress Effects 0.000 description 4
- 229930002875 chlorophyll Natural products 0.000 description 4
- 235000019804 chlorophyll Nutrition 0.000 description 4
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 4
- 238000003776 cleavage reaction Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 238000001976 enzyme digestion Methods 0.000 description 4
- 230000004345 fruit ripening Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000007017 scission Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 230000003859 lipid peroxidation Effects 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 241000589158 Agrobacterium Species 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000010195 expression analysis Methods 0.000 description 2
- 230000009746 freeze damage Effects 0.000 description 2
- 235000015203 fruit juice Nutrition 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- ARQXEQLMMNGFDU-JHZZJYKESA-N 4-methylumbelliferone beta-D-glucuronide Chemical compound C1=CC=2C(C)=CC(=O)OC=2C=C1O[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O ARQXEQLMMNGFDU-JHZZJYKESA-N 0.000 description 1
- JXCKZXHCJOVIAV-UHFFFAOYSA-N 6-[(5-bromo-4-chloro-1h-indol-3-yl)oxy]-3,4,5-trihydroxyoxane-2-carboxylic acid;cyclohexanamine Chemical compound [NH3+]C1CCCCC1.O1C(C([O-])=O)C(O)C(O)C(O)C1OC1=CNC2=CC=C(Br)C(Cl)=C12 JXCKZXHCJOVIAV-UHFFFAOYSA-N 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 102100020720 Calcium channel flower homolog Human genes 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 101000932468 Homo sapiens Calcium channel flower homolog Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 108020005089 Plant RNA Proteins 0.000 description 1
- 238000010802 RNA extraction kit Methods 0.000 description 1
- 241000915604 Scutellaria barbata Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000002247 constant time method Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000008641 drought stress Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 101150062587 flower gene Proteins 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 235000015094 jam Nutrition 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000008261 resistance mechanism Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Physiology (AREA)
- Immunology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a passion flower PeERF-2 gene, the nucleotide sequence of which is shown as SEQ ID NO. 1. The invention provides a passion flower PeERF-2 gene obtained by cloning passion flower for the first time, and the transcription level of the PeERF-2 gene in passion flower is up-regulated under drought, low temperature and other stresses, which shows that the gene can improve drought resistance, cold resistance and the like of plants, and researches show that the gene can also obviously improve the survivability of yeast under low temperature stress, promote early bolting and flowering of plants, reduce root hairs of plants, improve cold resistance and the like of plants. The invention provides a new candidate gene for the research on improving the stress resistance of yeast, regulating and controlling the plant growth, improving the stress resistance of plants and the like.
Description
Technical Field
The invention belongs to the technical field of genetic engineering, and particularly relates to a passion flower PeERF-2 gene and application thereof.
Background
Passion flower is a perennial evergreen climbing woody vine plant, is a fragrant and delicious fruit, and is known as the king of fruit juice. Also known as passion fruit, brazil fruit, bole, passion fruit, lotus seed, chrysanthemum, barbed skullcap, eggplant flower and hordei herba. The natural juice has bright color, unique and rich aromatic flavor and rich nutrition. The passion fruit has high nutritive value and the effects of preventing diseases and building body. The passion flower juice contains more than 60 volatile compounds, the content of soluble solid is up to 10-14%, the content of organic acid, amino acid vitamins and mineral elements is very rich, and the passion flower juice can be used as raw material to be processed into fruit juice, jam, jelly and other products, and has the effects of maintaining beauty and keeping young, clearing summer heat and promoting appetite, eliminating fatigue, refreshing, sobering up, diminishing inflammation and removing spots, reducing blood lipid and blood pressure, preventing arteriosclerosis and the like. As passion flower is increasingly used, research on molecular biology thereof is also being conducted and developed, and gene expression analysis is also being increasingly applied to reveal mechanisms of passion flower gene expression and regulation. Therefore, the method has important significance for positioning, cloning, functions and the like of important trait genes in the passion flower and for comprehensive development and utilization of the passion flower.
The passion flower is a warm-loving and light-loving plant which is generally cultivated in tropical and subtropical regions, the optimal growth environment temperature is about 20-30 ℃, the region with the average annual temperature above 18 ℃ is most suitable for planting, the average coldest month temperature is above 8 ℃, and the problem of freeze injury is frequently encountered in partial planting regions such as Guangxi, guizhou, fujian, yunnan and the like, and the influence area is about 30% of the domestic planting area. The growth and development of passion fruit are severely restricted by low temperature, so that water in tissue cells can be frozen and frosted, the tissue cells can be damaged or dead, leaves are injured when the leaves are light, leaves fall, the stems are frozen and cracked when the leaves are heavy, the roots are damaged, and the whole plant dies. The purple fruit variety can withstand a low temperature of-3 ℃, and only autumn-tip tender tissues show slight freeze injury. However, if the tree crown is left in a low-temperature environment below 0 ℃ for a long time, the tree crown is easily frozen and killed, and the tolerance to continuous low temperature is stronger than that of the passion fruit. The golden passion fruit grows slowly below 15 ℃, the growth is basically stopped below 10 ℃, the tender shoots below 6 ℃ are slightly cold, and the leaves and the tender shoots of the vines are dried up at 4 ℃, so that the cold resistance of the passion fruit is improved, the key factor for realizing the continuous healthy development of the passion fruit industry is also the key problem to be solved urgently in the development of the passion fruit industry. Therefore, the passion fruit gene with stress resistance is excavated, the regulation and control function of the passion fruit gene on improving the low temperature stress resistance of passion fruit is further verified, and a good foundation is provided for researching the resistance mechanism of passion fruit and utilizing the passion fruit gene for genetic improvement.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a passion flower PeERF-2 gene and application thereof.
In a first aspect, the invention provides a passion flower PeERF-2 gene, the nucleotide sequence of which is shown as SEQ ID NO. 1.
In a second aspect, the present invention provides a protein encoded by the PeERF-2 gene of passion flower according to the first aspect of the present invention.
In a third aspect, the present invention provides a recombinant vector comprising the coding region of the PeERF-2 gene of passion flower according to the first aspect of the present invention.
Wherein, the recombinant vector original vector can adopt vectors commonly used in the field of gene recombination, such as viruses, plasmids and the like. The invention is not limited in this regard. In one embodiment of the present invention, the primary vector is a pMD19-T vector, a pCAMBIA1304 expression vector, and a pYES2 expression vector, but it is understood that other plasmids, viruses, etc. may be used in the present invention.
Preferably, the original vector of the recombinant vector is a pCAMBIA1304 expression vector, and the passion flower PeERF-2 gene coding region is positioned between NcoI and SpeI restriction enzyme sites of the pCAMBIA1304 expression vector.
Preferably, the original vector of the recombinant vector is a pYES2 expression vector, and the passion flower PeERF-2 gene coding region is positioned between HinIII and BamHI restriction enzyme sites of the pYES2 expression vector.
In a fourth aspect of the present invention, there is provided a host bacterium comprising the coding region of the PeERF-2 gene of passion flower according to the first aspect.
In a fifth aspect, the present invention provides an expression cassette comprising the coding region of the PeERF-2 gene of passion flower according to the first aspect of the present invention.
In a sixth aspect, the present invention provides the use of the passion flower PeERF-2 gene according to the first aspect of the present invention, or the protein according to the second aspect of the present invention, or the recombinant vector according to the third aspect of the present invention, or the host bacterium according to the fourth aspect of the present invention, or the expression cassette according to the fifth aspect of the present invention, for improving cold resistance of yeast.
Preferably, the yeast is Saccharomyces cerevisiae, such as INVSc1 strain, and the like.
In a seventh aspect, the present invention provides the use of the passion flower PeERF-2 gene according to the first aspect of the present invention, or the protein according to the second aspect of the present invention, or the recombinant vector according to the third aspect of the present invention, or the host bacterium according to the fourth aspect of the present invention, or the expression cassette according to the fifth aspect of the present invention, for promoting early bolting of plants, and/or early flowering of plants, and/or reducing root hairs of plants.
In a specific embodiment of the invention, the plant is arabidopsis thaliana.
An eighth aspect of the present invention provides the use of the passion flower PeERF-2 gene according to the first aspect of the present invention, or the protein according to the second aspect of the present invention, or the recombinant vector according to the third aspect of the present invention, or the host bacterium according to the fourth aspect of the present invention, or the expression cassette according to the fifth aspect of the present invention, for improving drought resistance and/or cold resistance of plants.
A ninth aspect of the present invention provides a primer pair of ATGGATGATGTTTTCTCGAAC and CTATATGGAAAAGCTCCATAA; or the primer pair is CAGTATGGACTTTTGGTCGCTTG and CGCTTCTTGGGATAACTGGAA; or the primer pair is TGCCATGGATGGATGATGTTTTCTCGAA and CGGACTAGTTATGGAAAAGCTCCATAA; or the primer pair is CAAGCTTATGGATGATGTTTTCTCGAAC and CGGGATCCTATGGAAAAGCTCCATAA。
The invention provides a passion flower PeERF-2 gene obtained by cloning passion flower for the first time, and the transcription level of the PeERF-2 gene in passion flower is up-regulated under drought, low temperature and other stresses, which shows that the gene can improve drought resistance, cold resistance and the like of plants, and researches show that the gene can also obviously improve the survivability of yeast under low temperature stress, promote early bolting and flowering of plants, reduce root hairs of plants, improve cold resistance and the like of plants. The invention provides a new candidate gene for the research on improving the stress resistance of yeast, regulating and controlling the plant growth, improving the stress resistance of plants and the like.
Drawings
FIG. 1 shows qRT-PCR of PeERF-2 gene under different abiotic stress.
FIG. 2 shows qRT-PCR results of PeERF-2 gene at three fruit ripening stages.
FIG. 3 shows growth in yeast transformed with the PeERF-2 gene under low temperature stress.
FIG. 4 shows the phenotype of wild type and transgenic plants grown for 45 days.
FIG. 5 is root hair of wild type and transgenic plants.
FIG. 6 shows response of Arabidopsis thaliana transformed with PeERF-2 gene to low temperature stress.
FIG. 7 shows the phenotype of Arabidopsis thaliana transformed with PeERF-2 gene under low temperature stress.
FIG. 8 shows chlorophyll fluorescence detection results and physiological index measurement results of the PeERF-2 transgenic Arabidopsis under low temperature stress.
Detailed Description
The invention will be further described with reference to specific embodiments in order to provide a better understanding of the invention. The specific techniques or conditions are not identified in the examples and are performed according to techniques or conditions described in the literature in this field or according to the product specifications. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Example 1: cloning of passion flower PeERF-2 Gene
Extracting total RNA in passion fruit seedlings, and carrying out reverse transcription to obtain a cDNA first strand. PCR amplification was performed using the first strand of cDNA as template and ATGGATGATGTTTTCTCGAAC and CTATATGGAAAAGCTCCATAA primers.
The reaction system was a 25ul system of 1ul template, 1ul 5 'primer, 1ul,PCR mix 12.5ul 3' primer, and 9.5ul water.
The PCR amplification procedure was 94℃for 4 min,94℃for 30 seconds, 56℃for 1 min,72℃for 1 min, and 72℃for 10 min for 35 cycles.
Amplified products were recovered and cloned into pMD19-T vector (Promega, madison, wis., USA) (pMD 19-T-PeERF-2 vector) and sequenced on an ABI PRISM 310 genetic analyzer (PerkinElmer Applied Biosystems, foster City, calif., USA). Sequencing results show that the passion flower PeERF-2 gene is obtained through cloning, and the ORF sequence of the passion flower PeERF-2 gene is shown as SEQ ID NO. 1.
Example 2: expression analysis of passion flower PeERF-2 Gene
The passion fruit with purple fruit is named as TainongPassiflora edulis) The healthy passion flower seedlings of (C) were cultured in an incubator (30 ℃ C., 200. Mu. Mol. M) -2 ·s -1 Light intensity, 12 hours light/12 hours dark cycle, 70% relative humidity) to about 1 mHeight, and 8-10 functional blades. Plants were used for various abiotic stress treatments:
(1) drought stress: drought treatment is carried out on the plants, and samples are respectively taken when the soil moisture is divided into 50% and 10%;
(2) salt treatment: samples were taken 3 days and 10 days of treatment with 300mM saline solution, respectively;
(3) and (3) low-temperature treatment: plants were sampled except for 20 and 48 hours at 0deg.C;
(4) high temperature treatment: plants were placed at 45℃for 2, 4 and 24 hours, respectively, for sampling.
And detecting the gene expression condition of the PeERF-2 in the leaves under four abiotic stresses by using a real-time fluorescent quantitative PCR (qRT-PCR) technology. As a result, as shown in FIG. 1, peERF-2 was responsive to various abiotic stresses, peERF-2 was highly induced to be expressed at a soil moisture content of 10% at the time of drought treatment, peERF-2 was inhibited from being expressed under high temperature stress and salt stress, and PeERF2 was highly induced to be expressed under cold stress. The gene is related to the drought resistance and cold resistance of the passion flower, and can be used for improving the drought resistance and cold resistance of the passion flower and the like.
The passion fruit during ripening under normal growth conditions is sampled and three fruits at the stage of fruit ripening are selected (T1, two weeks before harvest, the pericarp is green; T2, the pericarp is red purple with no shrinkage upon harvest; T3, one week after harvest at 30 ℃ C., the pericarp has shrunk). The expression condition of the passion flower PeERF-2 gene in 3 stages of fruit ripening is detected by using a real-time fluorescence quantitative PCR (qRT-PCR) technology, the result is shown in a figure 2, the gene is in negative correlation with the fruit ripening, and the highest expression quantity is reached in a T1 stage.
Real-time fluorescent quantitative PCR (qRT-PCR) was performed as follows: tissues or leaves were frozen and total RNA was extracted in three biological replicates using a plant RNA isolation kit. qRT-PCR analysis was performed on Light 96 (Roche) using SYBR Premix Ex Taq ™ (TaKaRa, japan, tokyo) chemistry. The relative expression levels were calculated using the 2-fatter CT method. The primer is F CAGTATGGACTTTTGGTCGCTTG, the R CGCTTCTTGGGATAACTGGAA, PCR reaction system is a system of 1ul template, 1ul 5 'end primer, 1ul,PCR mix 12.5ul 3' end primer and 9.5ul water which are 25ul in total. The PCR reaction procedure was 94℃for 4 min,94℃for 30 seconds, 58℃for 1 min,72℃for 1 min, and 72℃for 10 min for 35 cycles.
Example 3: construction of recombinant vectors
The pMD19-T-PeERF-2 plasmid is used as a template, and a primer TG is used as a primerCCATGGATGGATGATGTTTTCTCGAAC (underlined as NcoI cleavage site) and CGGACTAGTTATGGAAAAGCTCCATAA (SpeI cleavage site underlined) was subjected to PCR amplification. The reaction system was a 25ul system of 1ul template, 1ul 5 'primer, 1ul,PCR mix 12.5ul 3' primer, and 9.5ul water. The reaction procedure was 94℃for 4 min,94℃for 30 seconds, 56℃for 1 min,72℃for 3 min,72℃for 10 min for 35 cycles. The amplified product is connected to the pCAMBIA1304 expression vector after the same enzyme digestion after the NcoI and SpeI double enzyme digestion, thus obtaining the pCAMBIA1304-PeERF-2 vector.
The pMD19-T-PeERF-2 vector is used as a template, and a primer C is usedAAGCTTATGGATGATGTTTTCTCGAAC (HinIII cleavage site) and CGGGATCCTATGGAAAAGCTCCATAA (BamHI cleavage site underlined) was amplified by PCR. The reaction system was a 25ul system of 1ul template, 1ul 5 'primer, 1ul,PCR mix 12.5ul 3' primer, and 9.5ul water. The reaction procedure was 94℃for 4 min,94℃for 30 seconds, 58℃for 1 min,72℃for 10 min, and a total of 35 cycles. The amplified product is connected to pYES2 expression vector after the same enzyme digestion after HinIII and BamHI double enzyme digestion, and pYES2-PeERF-2 vector is obtained.
Example 4: functional complementation of PeERF-2 in Yeast
pYES2-PeERF-2 and pYES2 vectors (control) were transfected into INVSc1 strain (Saccharomyces cerevisiae), respectively. For yeast complementation assay, yeast solution was cultured in SD-Ura liquid medium at 30℃and then subjected to cold stress treatment: cold stress experiments (-20 ℃ C. For 0 hours, 12 hours, 24 hours, 36 hours and 48 hours) were performed on pYES2-PeERF-2 and pYES2 empty vector (control), and the treated bacterial solutions were cultured in SD-Ura solid medium at 30 ℃. The experiment was repeated three times.
The transformation of pYES2-PeERF-2 and pYES2 empty vector (control) into INVSCl was subjected to low temperature stress, and the results are shown in FIG. 3 (1×,10×,100×,1000× in FIG. 3 represents dilution of bacterial liquid 1-fold, 10-fold, 100-fold, 1000-fold, respectively). The results show that the 24h, 36h and 48h growth conditions of the yeast transfected with the PeERF-2 gene are all superior to those of the control group under the cold stress. This result shows that PeERF-2 can improve cold resistance of transgenic yeast.
Example 5: phenotype of transgenic plants
The pCAMBIA1304-PeERF-2 was transformed into Agrobacterium, and the transformed Agrobacterium was then shaken at 28℃in YEB medium containing Kan and Rif antibiotics, respectively, and added to the Arabidopsis transgenic infiltration solution (1/2 MS,50 g/L) to 0 D600=0.8-1.0. Transformation of Arabidopsis using inflorescence impregnation we obtained the PeERF-2 transgenic Arabidopsis line.
The wild type and the obtained transgenic arabidopsis were respectively sown in vermiculite, and the phenotype of the transgenic arabidopsis was observed after 45 days, and the result shows that the transgenic arabidopsis grown normally for 45 days appears to be early bolting and early flowering compared with the wild type (figure 4). The transgenic and wild-type root lines were observed microscopically and found to have far fewer root hairs in the transgenic root system than the wild-type root system (fig. 5).
Example 6: low temperature stress treatment of transgenic plants
After the transgenic arabidopsis obtained in example 5 was grown for 14 days, a low temperature stress treatment was performed: the treatment was carried out in incubators at 4℃and 23℃for 36 hours, respectively. Fresh transgenic Arabidopsis samples under normal and low temperature conditions were placed in X-Gluc solution, in a water bath at 37℃in the dark for 12 hours, and chlorophyll from leaves was decolorized with absolute ethanol and photographed. Gus enzyme activity was determined by fluorescence of 4-methylumbelliferyl glucuronide. The results are shown in FIG. 6. Under normal growth conditions, transgenic arabidopsis GUS staining is mainly concentrated in the stems, whereas under low temperature stress conditions, GUS staining is enhanced and mainly concentrated in the leaves of seedlings. GUS enzyme activity was tested and it was found that the GUS activity of transgenic Arabidopsis after low temperature stress treatment was 3.4 times higher than that of the control. The result shows that the low-temperature stress has an induction effect on the PeERF-2, which indicates that the PeERF-2 can be used for improving cold resistance of plants and the like.
After the transgenic arabidopsis obtained in example 5 was grown for 25 days, after the treatment at low temperature of 0 ℃ for 10 hours, the effect of cold stress on the growth of transgenic plants and wild type plants was examined by using chlorophyll fluorescence imaging technique, and as shown in fig. 7, chlorophyll fluorescence images taken under low temperature conditions showed that all plants were more severely damaged than normal (the false color of the leaves of normal plants was blue, and the false color of the leaves was changed from green to yellow, even to black, indicating that the plants were more severely damaged), and the leaves of wild type and transgenic plants were blue under normal conditions, but when subjected to cold stress, most of the leaves of wild type were green or even black, and only some of the leaves of transgenic plants were green, and most of the leaves were blue, indicating that PeERF-2 could improve the cold tolerance of transgenic plants. Measurement of MDA (malondialdehyde), PRO (proline) and Ion leakage (Ion permeability) was performed on WT (wild type) and transgenic plants, and as a result, as shown in FIG. 8, membrane lipid peroxidation tends to occur when plants are subjected to stress in adversity, malondialdehyde is a final decomposition product of membrane lipid peroxidation, and the content thereof can reflect the degree to which plants suffer from adversity injury. In the research result, when the plants grow normally, the malondialdehyde content in the wild type and the transgenic plants is not greatly different, when the plants are subjected to low-temperature stress, the malondialdehyde content in the wild type and the transgenic plants is increased, compared with a control group, the malondialdehyde content in the wild type is increased more and higher than that of the transgenic plants, therefore, when the plants are subjected to low-temperature stress, the transgenic plants can reduce the membrane lipid peroxidation degree of the plants, and the cold resistance of the plants is improved. Under normal environmental conditions, the free proline content in the plant is low, while under stress, the free proline content in the plant is increased, and the accumulation amount of the free proline content is proportional to the stress resistance of the plant. In the research result, under the normal growth condition, the difference of the content of malondialdehyde in wild type and transgenic plants is smaller, and when the plants are subjected to low-temperature stress, the content of proline in the wild type and transgenic plants is increased, and the content of proline in the transgenic plants is obviously higher than that of the wild type, which indicates that PeERF-2 improves the cold resistance of transgenic Arabidopsis. In addition, when plants are stressed by adversity, the protoplasm structure can be influenced, the permeability of the protoplasm semipermeable membrane can be changed, and as adversity stress is increased, the ion permeability of the plants can be increased, so that the ion permeability of the plants can be measured to further indicate the stress resistance of the plants, and the plants with low ion permeability are generally stronger in adversity resistance. In this study, the ion permeability of wild type and transgenic plants was not much different during normal growth, and the ion permeability of each plant was increased when low temperature stress, whereas the wild type was much higher than that of transgenic plants, indicating that PeERF-2 was able to reduce ion permeability in plants and improve cold resistance of plants when low temperature stress.
The above description of the specific embodiments of the present invention has been given by way of example only, and the present invention is not limited to the above described specific embodiments. Any equivalent modifications and substitutions for this practical use will also occur to those skilled in the art, and are within the scope of the present invention. Accordingly, equivalent changes and modifications are intended to be included within the scope of the present invention without departing from the spirit and scope thereof.
Claims (8)
1. The passion flower PeERF-2 gene is characterized in that the nucleotide sequence is shown as SEQ ID NO. 1.
2. A protein encoded by the passion flower PeERF-2 gene of claim 1.
3. A recombinant vector or host bacterium or expression cassette comprising the coding region of the passion flower PeERF-2 gene of claim 1.
4. A recombinant vector according to claim 3, wherein the original vector of the recombinant vector is a pCAMBIA1304 expression vector and the coding region of the passion flower PeERF-2 gene is located between the NcoI and SpeI restriction enzyme sites of the pCAMBIA1304 expression vector.
5. A recombinant vector according to claim 3, wherein the original vector of the recombinant vector is a pYES2 expression vector and the coding region of the passion fruit PeERF-2 gene is located between the HinIII and BamHI restriction enzyme sites of the pYES2 expression vector.
6. Use of the passion flower PeERF-2 gene according to claim 1, or the protein according to claim 2, or the recombinant vector or host bacterium or expression cassette according to claim 3, or the recombinant vector according to claim 4 for improving cold resistance of yeast.
7. Use of the passion flower PeERF-2 gene according to claim 1, or the protein according to claim 2, or the recombinant vector or host bacterium or expression cassette according to claim 3, or the recombinant vector according to claim 4, for promoting early bolting, early flowering and/or reducing root hair in plants, which are arabidopsis thaliana.
8. Use of the passion flower PeERF-2 gene according to claim 1, or the protein according to claim 2, or the recombinant vector or host bacterium or expression cassette according to claim 3, or the recombinant vector according to claim 4 for improving drought and/or cold resistance of a plant, which is arabidopsis thaliana.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311188585.XA CN116926085B (en) | 2023-09-15 | 2023-09-15 | Passiflora edulis PeERF-2 gene and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311188585.XA CN116926085B (en) | 2023-09-15 | 2023-09-15 | Passiflora edulis PeERF-2 gene and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116926085A CN116926085A (en) | 2023-10-24 |
| CN116926085B true CN116926085B (en) | 2023-12-19 |
Family
ID=88377413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311188585.XA Active CN116926085B (en) | 2023-09-15 | 2023-09-15 | Passiflora edulis PeERF-2 gene and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116926085B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103387609A (en) * | 2012-05-11 | 2013-11-13 | 中国科学院上海生命科学研究院 | Gene capable of improving anti-stress capability of plants and application thereof |
| KR20220068335A (en) * | 2020-11-18 | 2022-05-26 | 대한민국(농촌진흥청장) | OsERF Gene enhancing heat or drought stress tolerance derived from Oryza sativa and uses thereof |
| CN116640780A (en) * | 2023-07-20 | 2023-08-25 | 中国热带农业科学院三亚研究院 | Passiflora edulis PeWOX-67 gene and application thereof |
-
2023
- 2023-09-15 CN CN202311188585.XA patent/CN116926085B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103387609A (en) * | 2012-05-11 | 2013-11-13 | 中国科学院上海生命科学研究院 | Gene capable of improving anti-stress capability of plants and application thereof |
| KR20220068335A (en) * | 2020-11-18 | 2022-05-26 | 대한민국(농촌진흥청장) | OsERF Gene enhancing heat or drought stress tolerance derived from Oryza sativa and uses thereof |
| CN116640780A (en) * | 2023-07-20 | 2023-08-25 | 中国热带农业科学院三亚研究院 | Passiflora edulis PeWOX-67 gene and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| Transcriptome-based identification and expression profiling of AP2/ERF members in Caragana intermedia and functional analysis of CiDREB3;Kun Liu 等;《Molecular Biology Reports》;第48卷;第7953-7965 * |
| Zhang,Q.等.GenBank: APQ47327.1.《GenBank》.2016,feature、origin部分. * |
| 西番莲不同种质资源抗寒性测定;郭玉琳 等;《热带作物学报》;第43卷(第5期);第955-963页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116926085A (en) | 2023-10-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110845590B (en) | Wild grape VyPPR gene and application of encoding protein thereof in drought stress | |
| CN116640780B (en) | Passiflora edulis PeWOX-67 gene and application thereof | |
| CN105255915B (en) | Application of the arabidopsis AtGDSL genes in the anti-sclerotiniose of rape and in promoting seed to sprout | |
| CN110950943B (en) | Alfalfa 'WL525' embryonic development late protein MsLEA-D34 and coding gene thereof | |
| CN109161550A (en) | A kind of the SlbHLH59 gene and application method of regulation tamato fruit ascorbic acid content | |
| CN106591322A (en) | Gingko MADS-box transcription factor gene GbMADS9 for controlling blossoming of plants and encoding protein and application of Gingko MADS-box transcription factor gene GbMADS9 | |
| CN107698673A (en) | Red palm AaMYB3 transcription factors and its encoding gene and application | |
| CN109468333A (en) | Citrus laccase family gene CsiLAC4 and its application | |
| CN111454972B (en) | Hovenia dulcis cold-resistant gene PtrBADH and application thereof in plant cold-resistant genetic improvement | |
| CN110452917B (en) | Wild grape VyGOLS gene and application of encoding protein thereof in drought stress | |
| CN109867716B (en) | Chimonanthus praecox CpVIN3 gene and application thereof | |
| CN116926085B (en) | Passiflora edulis PeERF-2 gene and application thereof | |
| CN114369616B (en) | Application of tomato SISPS gene in improving high temperature resistance of plants | |
| CN116926086B (en) | Passiflora edulis PeARF-1 gene and application thereof | |
| CN113564176B (en) | Wheat TaHAL3-7A gene and application thereof in regulating drought resistance of crops | |
| CN105585620B (en) | Soybean protein GmAIRP1 and its encoding gene are cultivating the application in resistance plant | |
| CN101144083A (en) | Descurainiasophia DsCBF gene, preparation method and application thereof | |
| CN112501142A (en) | Cryptomeria fortunei cold-resistant regulatory gene CfAPX and application thereof | |
| CN105695483B (en) | Larix olgensis LoERF017 gene and its amino acid sequence and application | |
| CN114875044B (en) | Wild grape VyVTE gene, protein coded by same and application thereof | |
| CN109906802A (en) | The screening technique of heat resistant type chrysanthemum | |
| CN114941003B (en) | Wild grape vyMPBQ gene, protein coded by same and application thereof | |
| CN113564182B (en) | Application of iris japonica SVP-like gene and method for obtaining iris japonica gene silencing or plant knockout | |
| NL2031934B1 (en) | Proanthocyanidin transport-related gst protein gene in brown cotton fiber and use thereof | |
| CN116286869B (en) | Application of feather needle grass sugar transport protein gene SpSWEET14 in improving cold resistance of plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |