CN116925879A

CN116925879A - Nucleic acid extraction equipment

Info

Publication number: CN116925879A
Application number: CN202210319371.0A
Authority: CN
Inventors: 解亚平; 符诚; 李江铧; 戴立忠
Original assignee: Sansure Biotech Inc
Current assignee: Sansure Biotech Inc
Priority date: 2022-03-29
Filing date: 2022-03-29
Publication date: 2023-10-24

Abstract

The present invention relates to a nucleic acid extraction apparatus. The nucleic acid extraction apparatus includes: a mounting frame having a sample zone, an orifice zone, and a reaction zone; the cover opening and closing device is arranged between the sample area and the pore plate area; first transfer means for transferring the sample tube to the switch cover means; the second transfer device is arranged in the pore plate area and is used for transferring the samples in the sample tube into the consumable of the pore plate; the extraction device is arranged between the orifice plate area and the reaction area, and the second transfer device is also used for transferring the orifice plate consumable to the extraction device; and the third transfer device is arranged in the reaction zone and is used for transferring the nucleic acid extracting solution into the reaction tube. In this way, the sample area and the pore plate area are physically isolated by using the switch cover device; the extraction device is used for physical isolation between the pore plate area and the reaction area, so that cross contamination caused by mutual influence of aerosol, liquid droplets and the like generated between the sample area and the pore plate area and between the pore plate area and the reaction area is avoided, and the accuracy of a nucleic acid detection result is improved.

Description

Nucleic acid extraction equipment

Technical Field

The invention relates to the technical field of biological detection, in particular to nucleic acid extraction equipment.

Background

The substance detected by the nucleic acid is typically a viral nucleic acid to determine whether the patient is infected with the virus by looking for the presence of foreign invading viral nucleic acid in the patient's respiratory tract specimen, blood or stool.

The nucleic acid extraction device is used for completing the steps of transferring a sample, extracting nucleic acid and the like. The nucleic acid extraction equipment is easy to generate aerosol, liquid spray and the like in the actual operation process, so that all areas are mutually influenced to cause cross contamination, and the accuracy of a nucleic acid detection result is further reduced.

Disclosure of Invention

In view of the above, it is necessary to provide a nucleic acid extraction apparatus that improves the above-described drawbacks, in order to solve the problems in the prior art that the nucleic acid extraction apparatus is prone to generate aerosol, liquid droplets, and the like during actual operation, and that the respective regions are affected with each other to cause cross contamination, and that the accuracy of the nucleic acid detection results is reduced.

A nucleic acid extraction apparatus comprising:

the mounting frame is provided with a sample area, an orifice plate area and a reaction area which are sequentially arranged at intervals along the first horizontal direction; the sample area is used for storing sample tubes, the pore plate area is used for storing pore plate consumables, and the reaction area is used for storing reaction tubes;

the switch cover device is arranged between the sample area and the pore plate area;

The first transfer device is arranged in the sample area and is used for transferring the sample tube of the sample area to the switch cover device so that the switch cover device can switch the sample tube;

the second transfer device is arranged in the pore plate area and is used for transferring the samples in the uncapped sample tube into the pore plate consumable of the pore plate area;

the second transfer device is also used for transferring the orifice plate consumable of the orifice plate region to the extraction device so that the extraction device can extract nucleic acid from a sample in the orifice plate consumable and obtain a nucleic acid extracting solution; a kind of electronic device with high-pressure air-conditioning system

And the third transfer device is arranged in the reaction zone and is used for transferring the nucleic acid extracting solution in the orifice plate consumable on the extraction device into the reaction tube of the reaction zone.

In one embodiment, the switch cover device comprises a clamping base and a rotary clamping jaw, wherein the clamping base and the rotary clamping jaw are arranged on the mounting frame, and the clamping base can move along the first horizontal direction relative to the mounting frame and sequentially pass through a first position, a second position and a third position; the first position is positioned on one side of the second position close to the sample area, the third position is positioned on one side of the second position close to the pore plate area, and the rotary clamping jaw is positioned above the second position;

The clamping base is used for receiving the sample tube transferred by the first transfer device when moving to the first position; when the clamping base moves to the second position, the rotary clamping jaw clamps the tube cover of the sample tube and performs screwing; when the clamping base moves to the third position, the sample in the sample tube on the clamping base is transferred by the second transfer device.

In one embodiment, the extracting device comprises a bearing base and an extracting mechanism, wherein the bearing base is arranged on the mounting frame, can move along the first horizontal direction relative to the mounting frame and sequentially passes through a fourth position, a fifth position and a sixth position; the fourth position is positioned on one side of the fifth position, which is close to the pore plate area, the sixth position is positioned on one side of the fifth position, which is close to the reaction area, and the extraction mechanism is positioned above the fifth position;

when the bearing base moves to the fourth position, the bearing base is used for bearing the orifice plate consumable transferred by the second transfer device; when the bearing base moves to the fifth position, the extracting mechanism extracts nucleic acid from the sample in the consumable of the pore plate on the bearing base; when the bearing base moves to the sixth position, the nucleic acid extracting solution in the orifice plate consumable on the bearing base is transferred by the third transfer device.

In one embodiment, the first transfer device comprises a first translation driving mechanism, a first translation seat, a second translation driving mechanism, a second translation seat and a first grabbing mechanism;

the first translation driving mechanism is arranged on the mounting frame, and the first translation seat is movably connected to the mounting frame along a second horizontal direction and is in driving connection with the first translation driving mechanism;

the second translation driving mechanism is arranged on the first translation seat, and the second translation seat is movably connected to the first translation seat along a third horizontal direction and is in driving connection with the second translation driving mechanism; the first grabbing mechanism is arranged on the second translation seat;

wherein the second horizontal direction intersects the third horizontal direction.

In one embodiment, the first transfer device further comprises a first lifting driving mechanism and a first lifting seat, wherein the first lifting seat is connected with the second translation seat in a lifting manner, and the first lifting driving mechanism is installed on the second translation seat and is in driving connection with the first lifting seat;

the first grabbing mechanism is arranged on the first lifting seat.

In one embodiment, the second transfer device comprises a third translation driving mechanism, a third translation seat, a fourth translation driving mechanism, a fourth translation seat and a first pipetting mechanism;

the third translation driving mechanism is arranged on the mounting frame, and the third translation seat is movably connected with the mounting frame along a third horizontal direction and is in driving connection with the third translation driving mechanism; the fourth translation driving mechanism is arranged on the third translation seat, and the fourth translation seat is movably connected with the third translation seat along the second horizontal direction and is in driving connection with the fourth translation driving mechanism; the first pipetting mechanism is arranged on the fourth translation seat;

In one embodiment, the second transferring device further comprises a second lifting driving mechanism and a second lifting seat, wherein the second lifting seat is connected to the fourth lifting seat in a lifting manner, and the second lifting driving mechanism is installed on the fourth lifting seat and is in driving connection with the second lifting seat;

the first pipetting mechanism is arranged on the second lifting seat.

In one embodiment, the second transferring device further comprises a fifth translation driving mechanism, a fifth translation seat and a second grabbing mechanism;

the fifth translation driving mechanism is arranged on the third translation seat, and the fifth translation seat is movably connected to the third translation seat along the second horizontal direction and is in driving connection with the fifth translation driving mechanism;

the second grabbing mechanism is arranged on the fifth translation seat.

In one embodiment, the second transferring device further includes a third lifting driving mechanism and a third lifting seat, wherein the third lifting seat is connected to the fifth translation seat in a lifting manner, and the third lifting driving mechanism is installed on the fifth translation seat and is in driving connection with the third lifting seat;

the second grabbing mechanism is arranged on the third lifting seat.

In one embodiment, the third transfer device further comprises a sixth translation driving mechanism, a sixth translation seat, a seventh translation driving mechanism, a seventh translation seat and a second pipetting mechanism;

the sixth translation driving mechanism is mounted on the mounting frame, and the sixth translation seat is movably connected with the mounting frame along a third horizontal direction and is in driving connection with the sixth translation driving mechanism; the seventh translation driving mechanism is arranged on the sixth translation seat, and the seventh translation seat is movably connected with the sixth translation seat along the second horizontal direction and is in driving connection with the seventh translation driving mechanism; the second pipetting mechanism is arranged on the seventh translation seat;

In one embodiment, the third transferring device further comprises an eighth translation seat, an eighth translation driving mechanism and a third pipetting mechanism;

the sixth translation seat is provided with a first sliding rail extending lengthwise along the second horizontal direction, the seventh translation seat is provided with a first sliding block, the eighth translation seat is provided with a second sliding block, and the first sliding block and the second sliding block are in sliding fit with the first sliding rail;

the eighth translation driving mechanism is arranged on the sixth translation seat and is in driving connection with the eighth translation seat; the third pipetting mechanism is arranged on the eighth translation seat.

In one embodiment, the third transfer device further comprises a ninth translation driving mechanism, a ninth translation seat and a fourth pipetting mechanism;

the ninth translation driving mechanism is arranged on the sixth translation seat, the ninth translation seat is movably connected with the sixth translation seat along the second horizontal direction and is in driving connection with the ninth translation driving mechanism, and the fourth pipetting mechanism is arranged on the ninth translation seat.

In one embodiment, the third transferring device further comprises a tenth translation seat, a tenth translation driving mechanism and a fifth pipetting mechanism;

the sixth translation seat is provided with a second sliding rail extending lengthwise along the second horizontal direction, the ninth translation seat is provided with a third sliding block, the tenth translation seat is provided with a fourth sliding block, and the third sliding block and the fourth sliding block are in sliding fit with the second sliding rail;

the tenth translation driving mechanism is installed on the sixth translation seat and is in driving connection with the tenth translation seat, and the fifth pipetting mechanism is arranged on the tenth translation seat.

In one embodiment, the third transferring device further comprises an eleventh translation driving mechanism, an eleventh translation seat and a third grabbing mechanism;

the eleventh translation driving mechanism is mounted on the sixth translation seat, the eleventh translation seat is movably connected to the sixth translation seat along the second horizontal direction and is in driving connection with the eleventh translation driving mechanism, and the third grabbing mechanism is arranged on the eleventh translation seat.

In practical use, the nucleic acid isolation apparatus described above first places a sample tube containing a sample in a sample area. The first transfer means transfers the sample tube of the sample zone to the switch cover means. After the sample tube is received by the cover opening and closing device, the cover is opened on the sample tube. After the cover is opened, the second transfer device transfers the sample in the sample tube after the cover is opened to the consumable orifice of the orifice plate area. Then, the second transfer device transfers the orifice plate consumable to the extraction device, and the extraction device performs nucleic acid extraction on the sample in the orifice plate consumable transferred onto the second transfer device, so that a nucleic acid extracting solution containing nucleic acid is obtained. After the nucleic acid extraction is completed, the third transfer device transfers the nucleic acid extracting solution in the consumable of the pore plate into the reaction tube of the reaction zone for subsequent nucleic acid amplification and detection.

Thus, the nucleic acid extraction equipment can automatically complete nucleic acid extraction, and the sample area and the pore plate area are physically isolated by using the switch cover device, so that cross contamination caused by the mutual influence of aerosol, liquid droplets and the like generated by the sample area and the pore plate area is avoided; the pore plate area and the reaction area are physically isolated by the extraction device, so that cross contamination caused by mutual influence of aerosol, liquid droplets and the like generated by the pore plate area and the reaction area is avoided, and the accuracy of a nucleic acid detection result is improved.

Drawings

FIG. 1 is a plan view of a nucleic acid isolation apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic diagram showing the structure of the nucleic acid isolation apparatus shown in FIG. 1;

FIG. 3 is a schematic view showing the structure of the nucleic acid isolation apparatus shown in FIG. 2 at another view angle;

FIG. 4 is a schematic diagram showing the structure of a second transfer means of the nucleic acid isolation apparatus shown in FIG. 2;

FIG. 5 is a schematic view of the second transfer device shown in FIG. 4 from another perspective;

FIG. 6 is a schematic structural view of a third transfer device of the nucleic acid isolation apparatus shown in FIG. 2;

fig. 7 is a schematic structural view of the third transfer device shown in fig. 6 at another view angle.

Detailed Description

In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the invention, whereby the invention is not limited to the specific embodiments disclosed below.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present invention, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.

In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present invention, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.

Referring to FIGS. 1 and 2, an embodiment of the present invention provides a nucleic acid isolation apparatus including a mounting frame 10, a cover opening and closing device 20, a first transfer device 30, a second transfer device 40, an extraction device 50, and a third transfer device 60.

The mounting frame 10 has a sample region 11, an orifice region 12, and a reaction region 13 sequentially arranged at intervals along a first horizontal direction X. The sample area 11 is used for storing a sample tube B1, the orifice area 12 is used for storing an orifice consumable B2, and the reaction area 13 is used for storing a reaction tube B3. The shutter device 20 is disposed between the sample region 11 and the well plate region 12, so that the sample region 11 and the well plate region 12 are physically isolated by the shutter device 20. The first transfer device 30 is disposed in the sample area 11, and is used for transferring the sample tube B1 of the sample area 11 to the cap opening and closing device 20, so that the cap opening and closing device 20 performs cap opening and closing on the sample tube B1 (i.e. unscrewing the tube cap on the sample tube B1 or screwing the tube cap on the sample tube B1). The second transfer device 40 is disposed in the orifice area 12, and is configured to transfer the sample in the uncapped sample tube B1 into the orifice consumable B2 of the orifice area 12. The extraction device 50 is disposed between the orifice plate region 12 and the reaction region 13 such that the orifice plate region 12 and the reaction region 13 are physically isolated by the extraction device 50. The second transferring device 40 is further configured to transfer the orifice plate consumable B2 of the orifice plate area 12 to the extracting device 50, so that the extracting device 50 performs nucleic acid extraction on the sample in the orifice plate consumable B2, and obtains a nucleic acid extracting solution containing nucleic acid. The third transfer device 60 is disposed in the reaction region 13, and is used for transferring the nucleic acid extracting solution in the orifice plate consumable B2 of the extraction device 50 into the reaction tube B3 of the reaction region 13 for subsequent nucleic acid amplification and detection (e.g. PCR fluorescent detection).

In actual use of the nucleic acid isolation apparatus, the sample tube B1 containing the sample is first placed in the sample region 11. The first transfer device 30 transfers the sample tube B1 of the sample area 11 to the opening and closing cover device 20. After receiving the sample tube B1, the opening and closing cap device 20 opens the cap of the sample tube B1. After the uncapping is completed, the second transfer device 40 transfers the sample in the uncapped sample tube B1 into the orifice plate consumable B2 of the orifice plate area 12. Then, the second transfer device 40 transfers the orifice plate consumable B2 to the extraction device 50 again, and the extraction device 50 performs nucleic acid extraction on the sample in the orifice plate consumable B2 transferred thereto, thereby obtaining a nucleic acid extraction solution containing nucleic acid. After the nucleic acid extraction is completed, the third transfer device 60 transfers the nucleic acid extracting solution in the orifice plate consumable B2 into the reaction tube B3 of the reaction zone 13 for subsequent nucleic acid amplification and detection.

Thus, the nucleic acid extraction equipment can automatically complete nucleic acid extraction, and the sample area 11 and the pore plate area 12 are physically isolated by the switch cover device 20, so that cross contamination caused by mutual influence of aerosol, liquid droplets and the like generated by the sample area 11 and the pore plate area 12 is avoided; the extraction device 50 is used for physical isolation between the orifice plate area 12 and the reaction area 13, so that cross contamination caused by mutual influence of aerosol, liquid droplets and the like generated by the orifice plate area 12 and the reaction area 13 is avoided, and the accuracy of a nucleic acid detection result is improved.

It should be noted that, in one embodiment, the well plate region 12 further has a location 122 where a reagent is placed, and the second transferring device 40 is further configured to transfer the reagent at the location 122 where the reagent is placed in the well plate region 12 into a corresponding reagent well of the well plate consumable B2, so as to perform nucleic acid extraction of the sample using the reagent.

It should be further noted that, in one embodiment, the reaction zone further has a location where the reaction solution is placed, and the third transfer device 60 is further configured to transfer the reaction solution at the location where the reaction solution is placed in the reaction zone into the reaction tube B3, so as to construct a reaction system.

In order to improve the working efficiency, in one embodiment, the number of the cover opening/closing devices 20 is two or more (two cover opening/closing devices 20 are shown in the embodiment shown in the drawings), and the two or more cover opening/closing devices 20 are sequentially arranged along the second horizontal direction Y perpendicular to the first horizontal direction X. The number of the extraction devices 50 is the same as the number of the opening and closing cover devices 20, and two or more extraction devices 50 are also sequentially arranged in the second horizontal direction Y. Thus, the operations of opening the cover, separating the cup, extracting the nucleic acid and constructing the reaction system can be simultaneously carried out, and the detection efficiency is improved.

In an embodiment of the present invention, the switch cover device 20 includes a clamping base 21 and a rotating jaw 22 provided on the mounting frame 10. The clamping base 21 is movable in a first horizontal direction X relative to the mounting frame 10 and is routed sequentially through a first position A1, a second position A2 and a third position A3. The first position A1 is located on the side of the second position A2 near the sample area 11, the third position A3 is located on the side of the second position A2 near the well plate area 12, and the rotating jaw 22 is located above the second position A2.

Thus, in actual use, the clamping base 21 is moved to the first position A1, and the sample tube B1 of the sample region 11 is transferred onto the clamping base 21 by the first transfer device 30. Then, the clamp base 21 is moved to the second position A2, and the sample tube B1 on the clamp base 21 is uncapped by the opening and closing cap device 20. Then, the holding base 21 is moved to the third position A3, and the sample in the sample tube B1 uncapped on the holding base 21 is transferred into the orifice consumable B2 of the orifice region 12 by the second transfer device 40, thereby completing uncapping and cup separating of the sample tube B1.

When the transfer of the sample in the sample tube B1 on the clamp base 21 is completed, the clamp base 21 is moved to the second position A2 again, and the empty sample tube B1 is capped by the cap opening and closing device 20. After closing the cap, the clamp base 21 is moved again to the first position A1, and the sample tube B1 above the clamp base 21 is transferred to the sample region 11 by the first transfer means 30. At this time, a new sample tube B1 may be transferred to the cap opening and closing device 20 by the first transfer device 30, and cap opening and cup separation may be performed again in the same manner as described above.

The clamp base 21 is capable of receiving the sample tube B1 and clamping or unclamping the sample tube B1. The rotating jaw 22 can be moved up and down to facilitate the opening and closing of the lid. Also, the rotating jaw 22 may be rotated so that the tube cap is unscrewed from the sample tube B1 (i.e., uncapped) or screwed onto the sample tube B1 after the rotating jaw 22 grips the tube cap.

In particular, in the embodiment, the nucleic acid extraction apparatus further includes a sample storage rack 111 provided in the sample region 11, and the sample tube B1 is stored by the sample storage rack 111. The specific structure of the sample storage rack 111 is not limited herein, as long as it is sufficient to store the sample tube B1.

In an embodiment of the present invention, the extraction device 50 includes a load-bearing base and an extraction mechanism disposed on the mounting frame 10. The bearing base is movable along the first horizontal direction X relative to the mounting frame 10, and sequentially passes through the fourth position A4, the fifth position A5 and the sixth position A6. The fourth position A4 is located on the side of the fifth position A5 near the orifice plate area 12, and the sixth position A6 is located on the side of the fifth position A5 near the reaction area 13. The extraction mechanism is located above the fifth position A5.

When the carrying base moves to the fourth position A4, the carrying base is used for carrying the orifice plate consumable B2 transferred by the second transfer device 40 (at this time, the orifice plate consumable B2 is filled with a sample). When the bearing base moves to a fifth position A5, the extraction mechanism extracts nucleic acid from the sample in the orifice consumable B2 on the bearing base. When the carrying base moves to the sixth position A6, the third transferring device 60 transfers the nucleic acid extracting solution in the orifice plate consumable B2 on the carrying base into the reaction tube B3 of the reaction zone 13.

Thus, in actual use, the carrying base is moved to the fourth position A4, and the orifice plate consumable B2 filled with the sample is transferred onto the carrying base by the second transfer device 40. Then, the carrying base is moved to a fifth position A5, and nucleic acid extraction is performed on the sample in the orifice plate consumable B2 on the carrying base by using the extraction mechanism (for example, nucleic acid extraction may be performed by a magnetic bead method). After the nucleic acid extraction is completed, the carrying base is moved to a sixth position A6, and the nucleic acid extracting solution in the orifice plate consumable B2 on the carrying base is transferred into the reaction tube B3 of the reaction zone 13 by the third transfer device 60.

Further, the reaction zone 13 is provided with a buffer carrier for buffering the orifice plate consumable B2. The third transferring device 60 is further configured to transfer the orifice plate consumable B2 located on the carrying base at the sixth position A6 onto the buffer carrier of the reaction zone 13. Thus, in actual use, the carrying base is moved to the fourth position A4, and the orifice plate consumable B2 filled with the sample is transferred onto the carrying base by the second transfer device 40. Then, the carrying base is moved to a fifth position A5, and nucleic acid extraction is performed on the sample in the orifice plate consumable B2 on the carrying base by using the extraction mechanism (for example, nucleic acid extraction may be performed by a magnetic bead method). After the nucleic acid extraction is completed, the carrying base is moved to a sixth position A6, and the orifice plate consumable B2 on the carrying base is transferred to the buffer carrier of the reaction zone 13 by using the third transfer device 60 (at this time, the carrying base can be moved to a fourth position A4 again and carries the orifice plate consumable B2). Then, the nucleic acid extracting solution in the orifice plate consumable B2 on the buffer carrier is transferred into the reaction tube B3 of the reaction region 13 by the third transfer device 60.

Further, the reaction zone 13 includes a waste liquid suction position. The third transfer device 60 is also used for transferring the waste liquid in the orifice plate consumable B2 located on the buffer carrier to the waste liquid suction position. The waste liquid refers to the liquid remaining in the consumable B2 of the well plate after the transfer of the nucleic acid extracting solution.

Further, the reaction zone 13 also includes a waste aperture plate discard location. The third transferring device 60 is further configured to transfer the orifice plate consumable B2 located on the buffer carrier to a discarding position of the waste orifice plate in the reaction area 13, so that the buffer carrier can receive the orifice plate consumable B2 again.

In the embodiment of the present invention, the first transferring device 30 includes a first translation driving mechanism 31, a first translation seat 32, a second translation driving mechanism 33, a second translation seat 34, and a first grasping mechanism 35.

The first translational drive mechanism 31 is mounted on the mounting frame 10. The first translation seat 32 is movably connected to the mounting frame 10 along the second horizontal direction Y and is in driving connection with the first translation driving mechanism 31, so that the first translation driving mechanism 31 can drive the first translation seat 32 to move along the second horizontal direction Y relative to the mounting frame 10.

The second translation driving mechanism 33 is mounted on the first translation seat 32. The second translation seat 34 is movably connected to the first translation seat 32 along the third horizontal direction and is in driving connection with the second translation driving mechanism 33, so that the second translation driving mechanism 33 can drive the second translation seat 34 to move along the third horizontal direction. The second horizontal direction Y intersects the third horizontal direction. The first gripping means 35 is mounted on the second translation stage 34 such that the first gripping means 35 can follow the second translation stage 34 in the second and third horizontal directions Y, thereby effecting transfer of the sample tube B1 of the sample zone 11 to the flip-top device 20 or transfer of an empty sample tube B1 on the flip-top device 20 to the sample zone 11. Preferably, the second horizontal direction Y is perpendicular to the third horizontal direction. In particular, in the embodiment shown in the drawings, the third horizontal direction is parallel to the first horizontal direction X, and the second horizontal direction Y is perpendicular to the first horizontal direction X.

Optionally, a guiding structure, such as a sliding rail and a sliding block, is disposed between the first translation seat 32 and the mounting frame 10, so as to guide the movement of the first translation seat 32 along the second horizontal direction Y relative to the mounting frame 10, so that the movement of the first translation seat 32 is more stable and reliable.

Optionally, a guiding structure, such as a sliding rail and a sliding block, is disposed between the second translation seat 34 and the first translation seat 32, so as to guide the movement of the second translation seat 34 along the third horizontal direction relative to the first translation seat 32, so that the movement of the second translation seat 34 is more stable and reliable.

Referring to fig. 3, in the embodiment, the first translation driving mechanism 31 includes a first translation driving member 311, a first driving wheel 312, a first driven wheel 313, and a first transmission belt 314. The first translation driving piece 311 is mounted on the mounting frame 10, and the first driving wheel 312 is mounted on an output shaft of the first translation driving piece 311, so that the first translation driving piece 311 can drive the first driving wheel 312 to rotate. The first driven pulley 313 is mounted on the mounting frame 10 and is spaced apart from the first driving pulley 312 in the second horizontal direction Y. The first driving belt 314 is sleeved between the first driving wheel 312 and the first driven wheel 313, and the first driving belt 314 is fixedly connected with the first translation seat 32.

Thus, when the first translation driving member 311 drives the first driving wheel 312 to rotate, the first driving wheel 312 drives the first driving belt 314 to sequentially move forward between the first driving wheel 312 and the first driven wheel 313, so that the first driving belt 314 drives the first translation seat 32 to move along the second horizontal direction Y, and further drives the second translation seat 34 and the first grabbing mechanism 35 to move along the second horizontal direction Y through the first translation seat 32. Alternatively, the first translation driving piece 311 may be a motor. The first driving pulley 312 and the first driven pulley 313 may be synchronous pulleys, and the first transmission belt 314 may be a synchronous belt.

In particular embodiments, the second translational drive mechanism 33 includes a second translational drive, a second drive pulley, a second driven pulley, and a second belt. The second translation driving member is mounted on the first translation seat 32, and the second driving wheel is mounted on an output shaft of the second translation driving member, so that the second translation driving member can drive the second driving wheel to rotate. The second driven wheel is mounted on the first translation seat 32 and is spaced apart from the second driving wheel along a third horizontal direction. The second driving belt is sleeved between the second driving wheel and the second driven wheel, and the second driving belt is fixedly connected with the second translation seat 34.

Thus, when the second translational driving piece drives the second driving wheel to rotate, the second driving wheel drives the second driving belt to sequentially move forward between the second driving wheel and the second driven wheel, so that the second driving belt drives the second translational seat 34 to move along the third horizontal direction, and the second translational seat 34 drives the first grabbing mechanism 35 to move along the third horizontal direction. Alternatively, the second translational drive may be a motor. The second driving pulley and the second driven pulley may be synchronous pulleys, and the second transmission belt may be a synchronous belt.

In the embodiment, the first transferring device 30 further includes a first lifting driving mechanism 37 and a first lifting seat 36. The first lifting base 36 is connected to the second lifting base 34 in a lifting manner, and the first grabbing mechanism 35 is disposed on the first lifting base 36. The first lifting driving mechanism 37 is drivingly connected between the first lifting seat 36 and the second translation seat 34, so that the first lifting driving mechanism 37 can drive the first lifting seat 36 to lift or lower relative to the second translation seat 34. Specifically, the first lifting driving mechanism 37 is mounted on the second translation seat 34, and is in driving connection with the first lifting seat 36.

In this way, when it is necessary to transfer the sample tube B1 to the opening and closing cover device 20, first, the first grasping mechanism 35 is moved above the sample tube B1 to be transferred of the sample region 11 in the second horizontal direction Y and/or the third horizontal direction by the first translational drive member 311 and/or the second translational drive member. Then, the first elevation driving mechanism 37 drives the first elevation seat 36 to move downward so that the first gripping mechanism 35 approaches the sample tube B1 until the first gripping mechanism 35 grips the sample tube B1. Then, the first lifting driving mechanism 37 drives the first lifting seat 36 to move upwards, and at this time, the first grabbing mechanism 35 drives the sample tube B1 to move upwards. Then, the first gripping mechanism 35 is moved above the gripping base 21 in the second horizontal direction Y and/or the third horizontal direction (at this time, the gripping base 21 is located at the first position A1) by the first translational driving member 311 and/or the second translational driving member. Then, the first elevating drive mechanism 37 drives the first elevating base 36 to move downward, so that the sample tube B1 grasped by the first grasping mechanism 35 is inserted into the holding base 21, and the holding base 21 grips the sample tube B1. Finally, the first grasping mechanism 35 releases the sample tube B1, and the first elevating drive mechanism 37 drives the first elevating seat 36 to move upward, so that the first grasping mechanism 35 is separated from the sample tube B1. Alternatively, the first gripping mechanism 35 may be a robot.

Further, the first lift driving mechanism 37 includes a first screw, a first lift driving member, and a first screw nut. The first screw rod is rotatably connected to the second translation seat 34 around its own axis, and the axial direction of the first screw rod is vertical. The first lifting driving piece is installed on the second translation seat 34 and is in driving connection with the first screw rod so as to drive the first screw rod to rotate around the axis of the first screw rod. The first screw nut is screwed on the first screw and is fixedly connected with the first lifting seat 36. Thus, when the first grabbing mechanism 35 needs to perform the lifting motion, the first lifting driving member drives the first screw rod to rotate, so as to drive the first screw rod nut and the first screw rod to move relatively along the axial direction (i.e. the vertical direction) of the first screw rod, and drive the first lifting seat 36 and the first grabbing mechanism 35 on the first lifting seat 36 to move relatively along the vertical direction (i.e. to rise or fall) with respect to the second translation seat 34. Alternatively, the first elevation driving member may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the first lifting seat 36 and the second lifting seat 34, so that the lifting movement of the first lifting seat 36 relative to the second lifting seat 34 is more stable and reliable.

Referring to fig. 3 to 5, in the embodiment of the invention, the second transferring device 40 includes a third translation driving mechanism 41, a third translation seat 42, a fourth translation driving mechanism 43, a fourth translation seat 44 and a first pipetting mechanism 46.

The third translational drive mechanism 41 is mounted on the mounting frame 10. The third translation seat 42 is movably connected to the mounting frame 10 along a third horizontal direction and is in driving connection with the third translation driving mechanism 41, so that the third translation driving mechanism 41 can drive the third translation seat 42 to move along the third horizontal direction. The fourth translation driving mechanism 43 is mounted on the third translation seat 42, and the fourth translation seat 44 is movably connected to the third translation seat 42 along the second horizontal direction Y and is in driving connection with the fourth translation driving mechanism 43, so that the fourth translation driving mechanism 43 can drive the fourth translation seat 44 to move along the second horizontal direction Y. The first pipetting mechanism 46 is disposed on the fourth translation stage 44 such that the first pipetting mechanism 46 can move along the third horizontal direction and the second horizontal direction Y along with the fourth translation stage 44, thereby sucking the sample in the sample tube B1 on the clamp base 21 at the third position A3, and transferring the sucked sample to the extraction device 50 so that the extraction device 50 extracts nucleic acid from the sample.

In the embodiment, the third translation driving mechanism 41 includes a third translation driving member 411, a third driving wheel 412, a third driven wheel 413, and a third transmission belt 414. The third translation driving member 411 is mounted on the mounting frame 10, and the third driving wheel 412 is mounted on an output shaft of the third translation driving member 411 such that the third translation driving member 411 can drive the third driving wheel 412 to rotate. The third driven wheel 413 is mounted on the mounting frame 10 and is spaced apart from the third driving wheel 412 in a third horizontal direction. The third driving belt 414 is sleeved between the third driving wheel 412 and the third driven wheel 413, so that the third driving belt 414 sequentially moves forward between the third driving wheel 412 and the third driven wheel 413 under the driving of the third driving wheel 412. The third driving belt 414 is fixedly connected with the third translation seat 42, so that the third driving belt 414 can drive the third translation seat 42 to move along the third horizontal direction.

In this way, when the third translation driving member 411 drives the third driving wheel 412 to rotate, the third driving belt 414 sequentially moves forward between the third driving wheel 412 and the third driven wheel 413, so as to drive the third translation seat 42 to move along the third horizontal direction, and further drive the first pipetting mechanism 46 to move along the third horizontal direction through the fourth translation seat 44. Alternatively, the third translation driving member 411 may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the third translation seat 42 and the mounting frame 10, so as to guide the movement of the third translation seat 42 along the third horizontal direction relative to the mounting frame 10, so that the movement of the third translation seat 42 is more stable and reliable.

In the embodiment, the fourth translational driving mechanism 43 includes a fourth translational driving member, a fourth driving wheel, a fourth driven wheel, and a fourth transmission belt. The fourth translation driving member is mounted on the third translation seat 42, and the fourth driving wheel is mounted on an output shaft of the fourth translation driving member such that the fourth translation driving member can drive the fourth driving wheel to rotate. The fourth driven wheel is mounted on the third translation seat 42 and is arranged at intervals along the second horizontal direction Y with the fourth driving wheel. The fourth driving belt is sleeved between the fourth driving wheel and the fourth driven wheel, so that the fourth driving belt sequentially moves forward between the fourth driving wheel and the fourth driven wheel under the drive of the fourth driving wheel. The fourth belt is fixedly connected to the fourth translation seat 44, such that the fourth belt is capable of driving the fourth translation seat 44 to move along the second horizontal direction Y.

Thus, when the fourth translational driving member drives the fourth driving wheel to rotate, the fourth driving belt sequentially moves forward between the fourth driving wheel and the fourth driven wheel, so as to drive the fourth translational seat 44 to move along the second horizontal direction Y, and further drive the first pipetting mechanism 46 to move along the second horizontal direction Y. Alternatively, the fourth translational drive may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the fourth translation seat 44 and the third translation seat 42 to guide the movement of the fourth translation seat 44 along the second horizontal direction Y relative to the third translation seat 42, so that the movement of the fourth translation seat 44 is more stable and reliable.

In the embodiment, the second transferring device 40 further includes a second lifting driving mechanism 45 and a second lifting seat 48. The second lifting seat 48 is connected to the fourth translation seat 44 in a lifting manner, and the second lifting driving mechanism 45 is connected between the second lifting seat 48 and the fourth translation seat 44 in a driving manner, so that the second lifting driving mechanism 45 can drive the second lifting seat 48 to lift or descend relative to the fourth translation seat 44. The first pipetting mechanism 46 is disposed on the second elevating seat 48 so as to ascend or descend along with the second elevating seat 48. Specifically, the second lifting driving mechanism 45 is mounted on the fourth translation seat 44, and is in driving connection with the second lifting seat 48.

Further, the well plate section 12 includes a position 121 where the gun head is placed and a position where the waste gun head is collected, so that the first pipetting mechanism 46 fetches the gun head at the position 121 where the gun head is placed each time before the sample is sucked; and, after each sample transfer is completed, the used gun heads are released to the position where the waste gun heads are collected, so that cross contamination of samples in different batches is avoided.

Thus, when the sample in the sample tube B1 needs to be transferred into the orifice consumable B2 of the orifice region 12, first, the first pipetting mechanism 46 is moved to the position 121 where the gun head is placed in the third horizontal direction and/or the second horizontal direction Y under the driving of the third translational driving member 411 and/or the fourth translational driving member, and then is lifted up after being lifted down by the driving of the second lifting driving mechanism 45, so as to pick up the gun head.

Then, the first pipetting mechanism 46 is moved above the gripping foot 21 in the third horizontal direction and/or the second horizontal direction Y (when the gripping foot 21 is in the third position A3) by the third and/or fourth translational drives 411 and/or 411. Then, the second elevation driving mechanism 45 drives the first pipetting mechanism 46 to descend so that the gun head on the first pipetting mechanism 46 is inserted into the sample tube B1 and suctions the sample. Then, the second elevation driving mechanism 45 drives the first pipetting mechanism 46 to ascend.

Then, the first pipetting mechanism 46 is moved above an orifice plate consumable B2 of the orifice plate area 12 in the third horizontal direction and/or the second horizontal direction Y under the drive of the third translation driving member 411 and/or the fourth translation driving member. Then, the second lifting driving mechanism 45 drives the first pipetting mechanism 46 to descend, so that the gun head on the first pipetting mechanism 46 is inserted into the orifice plate consumable B2, and the sample is injected into the orifice plate consumable B2. Then, the second elevation driving mechanism 45 drives the first pipetting mechanism 46 to ascend.

Finally, the first pipetting mechanism 46 is moved to a position for collecting the waste gun tips in the third horizontal direction and/or the second horizontal direction Y by the third translational driving member 411 and/or the fourth translational driving member, and is lowered and then raised by the second elevating driving member 45 to release the used gun tips to the position for collecting the waste gun tips. Alternatively, the first pipetting mechanism 46 may be a pipette.

Optionally, the second lifting driving mechanism 45 includes a second screw rod, a second lifting driving member, and a second screw nut. The second screw is rotatably connected to the fourth translation seat 44 around its own axis, and the axial direction of the second screw is the vertical direction. The second lifting driving member is mounted on the fourth translation seat 44 and is in driving connection with the second screw rod to drive the second screw rod to rotate around the axis thereof. The second screw nut is connected to the second screw by a screw thread and is fixedly connected with the second lifting seat 48. Thus, when the first pipetting mechanism 46 needs to perform the lifting movement, the second lifting driving member drives the second screw rod to rotate, so as to drive the second screw rod nut and the second screw rod to move relatively along the axial direction (i.e. the vertical direction) of the second screw rod, and drive the second lifting seat 48 and the first pipetting mechanism 46 on the second lifting seat 48 to move (i.e. lift or descend) relatively to the fourth translation seat 44 along the vertical direction. Alternatively, the second elevation driving member may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the second lifting seat 48 and the fourth translation seat 44, so that the lifting movement of the second lifting seat 48 relative to the fourth translation seat 44 is more stable and reliable.

In some embodiments, the second transfer device 40 further includes a fifth translation drive mechanism 472, a fifth translation mount 474, and a second grasping mechanism 47. The fifth translation driving mechanism 472 is mounted on the third translation seat 42, and the fifth translation seat 474 is movably connected to the third translation seat 42 along the second horizontal direction Y and is in driving connection with the fifth translation driving mechanism 472, so that the fifth translation driving mechanism 472 can drive the fifth translation seat 474 to move along the second horizontal direction Y relative to the third translation seat 42. The second grabbing mechanism 47 is disposed on the fifth translation seat 474, so that the second grabbing mechanism 47 can move along the third horizontal direction and the second horizontal direction Y along with the fifth translation seat 474, so that the second grabbing mechanism 47 can grab the orifice plate consumable B2 in the orifice plate area 12 and transfer the orifice plate consumable B2 onto the carrying base (at this time, the carrying base is located at the fourth position A4).

In particular embodiments, the fifth translation drive 472 includes a fifth translation drive, a fifth drive 4722, a fifth driven 4723, and a fifth drive belt 4724. The fifth translational drive is mounted on the third translational seat 42 and the fifth driver 4722 is mounted on the output shaft of the fifth translational drive such that the fifth translational drive is capable of driving the fifth driver 4722 to rotate. The fifth driven wheel 4723 is mounted on the third translation seat 42 and is spaced apart from the fifth driving wheel 4722 along the second horizontal direction Y. The fifth driving belt 4724 is sleeved between the fifth driving wheel 4722 and the fifth driven wheel 4723, so that the fifth driving belt 4724 sequentially moves forward between the fifth driving wheel 4722 and the fifth driven wheel 4723 under the driving of the fifth driving wheel 4722. The fifth driving belt 4724 is fixedly connected to the fifth translation seat 474, such that the fifth driving belt 4724 can drive the fifth translation seat 474 to move along the second horizontal direction Y.

In this way, when the fifth translational driving member drives the fifth driving wheel 4722 to rotate, the fifth driving belt 4724 sequentially moves forward between the fifth driving wheel 4722 and the fifth driven wheel 4723, so as to drive the fifth translational seat 474 to move along the second horizontal direction Y, and further drive the second grabbing mechanism 47 to move along the second horizontal direction Y. Alternatively, the fifth translational drive element may employ a motor.

In the embodiment, the second transferring device 40 further includes a third lifting driving mechanism 473 and a third lifting seat 471. The third elevating seat 471 is liftably connected to the fifth translation seat 474. The third lifting driving mechanism 473 is drivingly connected between the third lifting seat 471 and the fifth translation seat 474, so that the third lifting driving mechanism 473 can drive the third lifting seat 471 to ascend or descend relative to the fifth translation seat 474. The second grasping mechanism 47 is provided on the third lift seat 471 so that the second grasping mechanism 47 can move together with the third lift seat 471. Specifically, the third lifting driving mechanism 473 is mounted on the fifth translation seat 474 and is in driving connection with the third lifting seat 471.

Thus, when the orifice plate consumable B2 needs to be transferred, first, the second gripping mechanism 47 is moved above the orifice plate consumable B2 to be transferred in the orifice plate area 12 under the driving of the third and fifth translation driving members 411 and 411. The third lifting driving mechanism 473 drives the second grasping mechanism 47 to move downward until the second grasping mechanism 47 grasps the orifice plate consumable B2. Then, the third elevation driving mechanism 473 drives the second grasping mechanism 47 to move upward.

Then, the second gripping mechanism 47 is moved to above the carrying base (at this time, the carrying base is located at the fourth position A4) by the third and fifth translational driving members 411 and 411. Then, the third elevation driving mechanism 473 drives the second grasping mechanism 47 to move downward, and the second grasping mechanism 47 releases the orifice plate consumable B2 onto the bearing base. Then, the third elevation driving mechanism 473 drives the second grasping mechanism 47 to move upward. Alternatively, the second gripping mechanism 47 may be a robot.

Further, the third lifting driving mechanism 473 includes a third screw rod, a third lifting driving member, and a third screw rod nut. The third screw is rotatably connected to the fifth translation seat 474 about its own axis, and the axial direction of the third screw is the vertical direction. The third lifting driving member is mounted on the fifth translation seat 474 and is in driving connection with the third screw rod to drive the third screw rod to rotate around the axis thereof. The third screw nut is connected to the third screw rod through a thread and is fixedly connected with the third lifting seat 471. Thus, when the second grabbing mechanism 47 needs to perform the lifting movement, the third lifting driving member drives the third screw rod to rotate, so as to drive the third screw rod nut and the third screw rod to move relatively along the axial direction (i.e. the vertical direction) of the third screw rod, and drive the third lifting seat 471 and the second grabbing mechanism 47 on the third lifting seat 471 to move (i.e. rise or fall) relatively to the fifth translation seat 474 along the vertical direction. Alternatively, the third elevating driving member may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the third lifting seat 471 and the fifth translation seat 474, so that the lifting movement of the third lifting seat 471 relative to the fifth translation seat 474 is more stable and reliable.

Referring to fig. 3, 6 and 7, in the embodiment of the invention, the third transferring device 60 further includes a sixth translation driving mechanism 66, a sixth translation seat 67, a seventh translation driving mechanism 681, a seventh translation seat 611 and a second pipetting mechanism 61.

The sixth translation driving mechanism 66 is mounted on the mounting frame 10, and the sixth translation seat 67 is movably connected to the mounting frame 10 along the third horizontal direction and is in driving connection with the sixth translation driving mechanism 66, so that the sixth translation driving mechanism 66 can drive the sixth translation seat 67 to move along the third horizontal direction. The seventh translation driving mechanism 681 is mounted on the sixth translation seat 67, and the seventh translation seat 611 is movably connected to the sixth translation seat 67 along the second horizontal direction Y and is in driving connection with the seventh translation driving mechanism 681, so that the seventh translation driving mechanism 681 can drive the seventh translation seat 611 to move along the second horizontal direction Y. The second pipetting mechanism 61 is disposed on the seventh translation seat 611, so that the second pipetting mechanism 61 can move along the third horizontal direction and the second horizontal direction Y along with the seventh translation seat 611, and the second pipetting mechanism 61 can transfer the nucleic acid extracting solution in the orifice plate consumable B2 on the carrier base into the reaction tube B3 of the reaction zone 13.

In particular embodiments, the sixth translational drive mechanism 66 includes a sixth translational drive member 661, a sixth drive wheel 662, a sixth driven wheel 663, and a sixth belt 664. The sixth translational driving member 661 is mounted on the mounting frame 10, and the sixth driving wheel 662 is mounted on an output shaft of the sixth translational driving member 661 such that the sixth translational driving member 661 can drive the sixth driving wheel 662 to rotate. The sixth driven wheel 663 is mounted on the mounting frame 10 and is disposed at a distance from the sixth driving wheel 662 in the third horizontal direction. The sixth driving belt 664 is sleeved between the sixth driving wheel 662 and the sixth driven wheel 663, so that the sixth driving belt 664 sequentially moves forward between the sixth driving wheel 662 and the sixth driven wheel 663 under the driving of the sixth driving wheel 662. The sixth driving belt 664 is fixedly connected with the sixth translation seat 67, so that the sixth driving belt 664 can drive the sixth translation seat 67 to move along the third horizontal direction. Alternatively, the sixth translational driving member 661 may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, is disposed between the sixth translation seat 67 and the mounting frame 10, so as to guide the movement of the sixth translation seat 67 along the third horizontal direction relative to the mounting frame 10, so that the movement of the sixth translation seat 67 is more stable and reliable.

In the embodiment, the seventh translational driving mechanism 681 includes a seventh translational driving member, a seventh driving wheel, a seventh driven wheel 6811, and a seventh transmission belt 6812. The seventh translational driving member is mounted on the sixth translational seat 67, and the seventh driving wheel is mounted on the output shaft of the seventh translational driving member, so that the seventh translational driving member can drive the seventh driving wheel to rotate. The seventh driven wheel 6811 is mounted on the sixth translation seat 67 and is spaced apart from the seventh driving wheel along the second horizontal direction Y. The seventh driving belt 6812 is sleeved between the seventh driving wheel and the seventh driven wheel 6811, so that the seventh driving belt 6812 sequentially moves forward between the seventh driving wheel and the seventh driven wheel 6811 under the driving of the seventh driving wheel. The seventh driving belt 6812 is fixedly connected to the seventh translation seat 611, so that the seventh driving belt 6812 can drive the seventh translation seat 611 to move along the second horizontal direction Y. Alternatively, the seventh translational drive may employ a motor.

In particular, in the embodiment, the third transferring device 60 further includes a fourth lifting driving mechanism 612 and a fourth lifting seat. The fourth lifting base is connected to the seventh translation base 611 in a lifting manner. The fourth lifting driving mechanism 612 is drivingly connected between the fourth lifting seat and the seventh translation seat 611, so that the fourth lifting driving mechanism 612 can drive the fourth lifting seat to ascend or descend relative to the seventh translation seat 611. The second pipetting mechanism 61 is arranged on the fourth lifting seat so that the second pipetting mechanism 61 can move together with the fourth lifting seat. Specifically, the fourth elevating driving mechanism 612 is mounted on the seventh translation seat 611 and is in driving connection with the fourth elevating seat.

Further, the reaction zone 13 includes a position 131 where the tip is placed and a position where the waste tip is collected, so that the second pipetting mechanism 61 fetches the tip at the position 131 where the tip is placed each time before the nucleic acid extracting solution is sucked; and, after completing the transfer of nucleic acid extract each time, the used tips are released to the position where the waste tips are collected, thereby avoiding cross contamination of nucleic acid extracts of different batches.

Thus, when the nucleic acid extracting solution in the orifice plate consumable B2 needs to be transferred, first, the second pipetting mechanism 61 is moved to the position 131 where the gun head is placed in the reaction area 13 in the third horizontal direction and/or the second horizontal direction Y under the driving of the sixth translational driving mechanism 66 and the seventh translational driving mechanism 681, and the second pipetting mechanism 61 is lowered to pick up the gun head and is raised to reset under the driving of the fourth lifting driving mechanism 612.

Then, the second pipetting mechanism 61 is moved in the third horizontal direction and/or the second horizontal direction Y to above the orifice plate consumable B2 on the carrier base (at this time, the carrier base is located at the sixth position A6) under the drive of the sixth translational driving mechanism 66 and/or the seventh translational driving mechanism 681. The fourth lifting driving mechanism 612 drives the second pipetting mechanism 61 to move downwards until the gun head on the second pipetting mechanism 61 is inserted into a reagent hole of the orifice plate consumable B2 and suctions the nucleic acid extracting solution. After that, the fourth elevation drive mechanism 612 drives the second pipetting mechanism 61 to move upward.

Then, the second pipetting mechanism 61 is moved in the third horizontal direction and/or the second horizontal direction Y to above a reaction tube B3 of the reaction zone 13 by the sixth and/or seventh translational driving mechanisms 66 and 681. The fourth elevating driving mechanism 612 drives the second pipetting mechanism 61 to move downward until the gun head on the second pipetting mechanism 61 is inserted into the reaction tube B3 and the nucleic acid extracting solution is injected into the reaction tube B3. After that, the fourth elevation drive mechanism 612 drives the second pipetting mechanism 61 to move upward.

Finally, the second pipetting mechanism 61 is moved in the third horizontal direction and/or the second horizontal direction Y to above the position of the reaction zone 13 where the waste gun heads are collected, driven by the sixth translational driving mechanism 66 and/or the seventh translational driving mechanism 681. The fourth elevation drive mechanism 612 drives the second pipetting mechanism 61 downward until the second pipetting mechanism 61 releases the used gun heads to a position where the used gun heads are collected. After that, the fourth elevation drive mechanism 612 drives the second pipetting mechanism 61 to move upward.

Further, the fourth lift drive mechanism 612 includes a fourth lead screw, a fourth lift drive, and a fourth lead screw nut. The fourth screw rod is rotatably connected to the seventh translation seat 611 around its own axis, and the axial direction of the fourth screw rod is in the vertical direction. The fourth lifting driving piece is mounted on the seventh translation seat 611 and is in driving connection with the fourth screw rod to drive the fourth screw rod to rotate around the axis of the fourth screw rod. The fourth screw nut is connected to the fourth screw rod in a threaded manner and is fixedly connected with the fourth lifting seat. Thus, when the second pipetting mechanism 61 needs to perform the lifting movement, the fourth lifting driving member drives the fourth screw rod to rotate, so as to drive the fourth screw rod nut and the fourth screw rod to move relatively in the axial direction (i.e. vertical direction) of the fourth screw rod, and drive the fourth lifting seat and the second pipetting mechanism 61 on the fourth lifting seat to move (i.e. lift or descend) in the vertical direction relative to the seventh translation seat 611. Alternatively, the fourth elevating drive may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the fourth lifting seat and the seventh translation seat 611, so that the lifting movement of the fourth lifting seat relative to the seventh translation seat 611 is more stable and reliable.

In some embodiments, the third transfer device 60 further includes an eighth translation stage 621, an eighth translation drive mechanism 682, and a third pipetting mechanism 62 (see fig. 2). The sixth translation seat 67 is provided with a first sliding rail a1 extending lengthwise along the second horizontal direction Y. The seventh translation seat 611 is provided with a first sliding block a2, the eighth translation seat 621 is provided with a second sliding block, and the first sliding block a2 and the second sliding block are both in sliding fit with the first sliding rail a1. The eighth translation driving mechanism 682 is mounted on the sixth translation seat 67 and is in driving connection with the eighth translation seat 621, such that the eighth translation driving mechanism 682 can drive the eighth translation seat 621 to move along the second horizontal direction Y relative to the sixth translation seat 67. The third pipetting mechanism 62 is disposed on the eighth translation seat 621, so that the third pipetting mechanism 62 moves along the third horizontal direction and the second horizontal direction Y along with the eighth translation seat 621, and the third pipetting mechanism 62 can transfer the nucleic acid extracting solution in the orifice plate consumable B2 on the carrier base into the reaction tube B3 of the reaction zone 13. Thus, the second pipetting mechanism 61 and the third pipetting mechanism 62 can be utilized to simultaneously aspirate nucleic acid extracts in different reagent wells of the well plate consumable B2 and transfer the nucleic acid extracts into the corresponding reaction tubes B3 at the same time, so that single-channel or double-channel pipetting can be realized. Moreover, the second pipetting mechanism 61 and the third pipetting mechanism 62 are jointly arranged on the same first slide rail a1, so that the second pipetting mechanism 61 and the third pipetting mechanism 62 can be close to each other as much as possible, and compatibility of the orifice plate consumable B2 with different orifice spacing is improved. Alternatively, the second pipetting mechanism 61 and the third pipetting mechanism 62 may be pipettes.

Specifically, in one embodiment, the eighth translational drive mechanism 682 includes an eighth translational drive, an eighth drive pulley, an eighth driven pulley 6821, and an eighth belt 6822. The eighth translation driving member is mounted on the sixth translation seat 67, and the eighth driving wheel is mounted on an output shaft of the eighth translation driving member, so that the eighth translation driving member can drive the eighth driving wheel to rotate. The eighth driven wheel 6821 is mounted on the sixth translation seat 67 and is spaced apart from the eighth driving wheel along the second horizontal direction Y. The eighth transmission belt 6822 is sleeved between the eighth driving wheel and the eighth driven wheel 6821, so that the eighth transmission belt 6822 sequentially moves forward between the eighth driving wheel and the eighth driven wheel 6821 under the drive of the eighth driving wheel. The eighth belt 6822 is fixedly connected to the eighth translation seat 621, such that the eighth belt 6822 can drive the eighth translation seat 621 and the third pipetting mechanism 62 on the eighth translation seat 621 to move along the second horizontal direction Y. Alternatively, the eighth translational drive element may employ a motor.

Further, the third transferring device 60 further includes a fifth lifting driving mechanism 622 and a fifth lifting seat. The fifth lifting seat is connected to the eighth translation seat 621 in a lifting manner. The fifth lifting driving mechanism 622 is drivingly connected between the fifth lifting seat and the eighth translation seat 621, so that the fifth lifting driving mechanism 622 can drive the fifth lifting seat to ascend or descend relative to the eighth translation seat 621. The third pipetting mechanism 62 is disposed on the fifth lifting seat such that the third pipetting mechanism 62 can move along with the fifth lifting seat. In this way, the fifth lifting driving mechanism 622 can drive the fifth lifting seat to move up and down, so as to drive the third pipetting mechanism 62 to move up and down, so that the third pipetting mechanism 62 can aspirate the nucleic acid extracting solution in the orifice plate consumable B2 or inject the aspirated nucleic acid extracting solution into the reaction tube B3. Specifically, the fifth lifting driving mechanism 622 is mounted on the eighth translation seat 621 and is in driving connection with the fifth lifting seat.

Further, the fifth lifting driving mechanism 622 includes a fifth screw rod, a fifth lifting driving member, and a fifth screw nut. The fifth screw rod is rotatably connected to the eighth translation seat 621 around its own axis, and the axial direction of the fifth screw rod is in the vertical direction. The fifth lifting driving piece is installed on the eighth translation seat 621 and is in driving connection with the fifth screw rod so as to drive the fifth screw rod to rotate around the axis of the fifth screw rod. The fifth screw nut is in threaded connection with the fifth screw rod and is fixedly connected with the fifth lifting seat. In this way, when the third pipetting mechanism 62 needs to perform the lifting movement, the fifth lifting driving member drives the fifth screw rod to rotate, so as to drive the fifth screw rod nut and the fifth screw rod to move relatively in the axial direction (i.e. the vertical direction) of the fifth screw rod, and drive the fifth lifting seat and the third pipetting mechanism 62 on the fifth lifting seat to move (i.e. lift or descend) relatively to the eighth translation seat 621 in the vertical direction. Alternatively, the fifth elevating driving member may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the fifth lifting seat and the eighth translation seat 621, so that the lifting movement of the fifth lifting seat relative to the eighth translation seat 621 is more stable and reliable.

In particular, in the embodiment, the third transfer device 60 further includes a ninth translation driving mechanism 683, a ninth translation seat 631, and a fourth pipetting mechanism 63. The ninth translation driving mechanism 683 is disposed on the sixth translation seat 67, and the ninth translation seat 631 is movably connected to the sixth translation seat 67 along the second horizontal direction Y and is in driving connection with the ninth translation driving mechanism 683, so that the ninth translation driving mechanism 683 can drive the ninth translation seat 631 to move along the second horizontal direction Y relative to the sixth translation seat 67. The fourth pipetting mechanism 63 is disposed on the ninth translation seat 631, so that the fourth pipetting mechanism 63 can move along the third horizontal direction and the second horizontal direction Y along with the ninth translation seat 631, and the fourth pipetting mechanism 63 can transfer the nucleic acid extracting solution in the orifice plate consumable B2 on the carrier to the reaction tube B3 of the reaction zone 13. Thus, the second pipetting mechanism 61, the third pipetting mechanism 62 and the fourth pipetting mechanism 63 can be used for pipetting simultaneously, and three-channel pipetting can be achieved.

Further, the ninth translational drive mechanism 683 includes a ninth translational drive, a ninth drive pulley, a ninth driven pulley 6832, and a ninth belt 6831. The ninth translation driving member is mounted on the sixth translation seat 67 and the ninth capstan is mounted on the output shaft of the sixth translation driving member 661 such that the ninth translation driving member can drive the ninth capstan to rotate. The ninth driven wheel 6832 is mounted on the sixth translation seat 67 and is spaced apart from the ninth driving wheel along the second horizontal direction Y. The ninth transmission belt 6831 is sleeved between the ninth driving wheel and the ninth driven wheel 6832, so that the ninth transmission belt 6831 sequentially moves forward between the ninth driving wheel and the ninth driven wheel 6832 under the drive of the ninth driving wheel. The ninth belt 6831 is fixedly connected to the ninth translation seat 631, such that the ninth belt 6831 is capable of driving the ninth translation seat 631 to move along the second horizontal direction Y. Alternatively, the ninth translational drive element may employ a motor.

Further, the third transferring device 60 further includes a sixth lifting driving mechanism 632 and a sixth lifting seat. The sixth lifting seat is connected to the ninth translation seat 631 in a lifting manner. The sixth lifting driving mechanism 632 is drivingly connected between the sixth lifting seat and the ninth translation seat 631, so that the sixth lifting driving mechanism 632 can drive the sixth lifting seat to ascend or descend relative to the ninth translation seat 631. The fourth pipetting mechanism 63 is arranged on the sixth lifting seat so that the fourth pipetting mechanism 63 can move together with the sixth lifting seat. Specifically, the sixth elevating driving mechanism 632 is mounted on the ninth translation seat 631 and is drivingly connected to the sixth elevating seat. Thus, the sixth lifting driving mechanism 632 can drive the sixth lifting seat to move up and down, so as to drive the fourth pipetting mechanism 63 to move up and down, thereby facilitating the fourth pipetting mechanism 63 to aspirate the nucleic acid extracting solution in the orifice plate consumable B2 or inject the aspirated nucleic acid extracting solution into the reaction tube B3.

Further, the sixth lift driving mechanism 632 includes a sixth screw rod, a sixth lift driving member, and a sixth screw nut. The sixth screw is rotatably connected to the ninth translation seat 631 about its own axis, and the axial direction of the sixth screw is a vertical direction. The sixth lifting driving piece is mounted on the ninth translation seat 631 and is in driving connection with the sixth screw rod to drive the sixth screw rod to rotate around the axis of the sixth screw rod. The sixth screw nut is connected to the sixth screw rod in a threaded manner and is fixedly connected with the sixth lifting seat. In this way, when the fourth pipetting mechanism 63 needs to perform the lifting movement, the sixth lifting driving member drives the sixth screw rod to rotate, so as to drive the sixth screw rod nut and the sixth screw rod to move relatively in the axial direction (i.e. the vertical direction) of the sixth screw rod, and drive the sixth lifting seat and the fourth pipetting mechanism 63 on the sixth lifting seat to move (i.e. rise or fall) relatively to the ninth translation seat 631 in the vertical direction. Alternatively, the sixth elevating driving member may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the sixth lifting seat and the ninth translation seat 631, so that the lifting movement of the sixth lifting seat relative to the ninth translation seat 631 is more stable and reliable.

In particular, in the embodiment, the third transferring device 60 further includes a tenth translation seat 641, a tenth translation driving mechanism 684, and a fifth pipetting mechanism 64. The sixth translation seat 67 is provided with a second sliding rail a3 extending lengthwise along the second horizontal direction Y, the ninth translation seat 631 is provided with a third sliding block a4, the tenth translation seat 641 is provided with a fourth sliding block, and the third sliding block a4 and the fourth sliding block are in sliding fit with the second sliding rail a 3. In this way, the movement of the ninth translation seat 631 relative to the sixth translation seat 67 along the second horizontal direction Y is guided by the movement of the third slider a4 along the second slide rail a 3; the movement of the tenth translation seat 641 with respect to the sixth translation seat 67 in the second horizontal direction Y is guided by the movement of the fourth slider along the second slide rail a 3.

The tenth translation driving mechanism 684 is mounted on the sixth translation seat 67 and is in driving connection with the tenth translation seat 641, such that the tenth translation driving mechanism 684 can drive the tenth translation seat 641 to move along the second horizontal direction Y relative to the sixth translation seat 67. The fifth pipetting mechanism 64 is disposed on the tenth translation seat 641, so that the fifth pipetting mechanism 64 can move along the third horizontal direction and the second horizontal direction Y along with the tenth translation seat 641, and further the fifth pipetting mechanism 64 transfers the nucleic acid extracting solution in the orifice plate consumable B2 on the carrier to the reaction tube B3 of the reaction zone 13. As such, one or more of the second pipetting mechanism 61, the third pipetting mechanism 62, the fourth pipetting mechanism 63 and the fifth pipetting mechanism 64 may be utilized to aspirate nucleic acid extracts within different reagent wells of the well plate consumable B2 and transfer into the corresponding reaction tube B3, enabling single-, dual-, three-or four-channel pipetting. And, second pipetting mechanism 61 and third pipetting mechanism 62 set up jointly on same first slide rail a1 to be favorable to making second pipetting mechanism 61 and third pipetting mechanism 62 be close to each other as far as possible, be favorable to promoting the compatibility to orifice plate consumptive material B2 that the hole interval size is different. Similarly, the fourth pipetting mechanism 63 and the fifth pipetting mechanism 64 are jointly disposed on the same second slide rail a3, so that the fourth pipetting mechanism 63 and the fifth pipetting mechanism 64 can be as close to each other as possible, and compatibility of the orifice plate consumable B2 with different orifice distances can be improved. Optionally, the fourth pipetting mechanism 63 and the fifth pipetting mechanism 64 are pipettes.

In particular embodiments, the tenth translational drive mechanism 684 includes a tenth translational drive, a tenth drive pulley, a tenth driven pulley 6842, and a tenth belt 6841. The tenth translational drive member is mounted on the sixth translational seat 67, and the tenth drive wheel is mounted on the output shaft of the tenth translational drive member such that the tenth translational drive member is capable of driving the tenth drive wheel to rotate. The tenth driven wheel 6842 is mounted on the sixth translation seat 67 and is spaced apart from the tenth driving wheel along the second horizontal direction Y. The tenth transmission belt 6841 is sleeved between the tenth driving wheel and the tenth driven wheel 6842, so that the tenth transmission belt 6841 sequentially moves forward between the tenth driving wheel and the tenth driven wheel 6842 under the drive of the tenth driving wheel. The tenth transmission belt 6841 is fixedly connected with the tenth translation seat 641 such that the tenth transmission belt 6841 can drive the tenth translation seat 641 to move along the second horizontal direction Y. Alternatively, the tenth translational drive may employ a motor.

Further, the third transfer device 60 further includes a seventh elevating driving mechanism 642 and a seventh elevating seat. The seventh elevating seat is liftably connected to the tenth translation seat 641. The seventh elevating driving mechanism 642 is drivingly connected between the seventh elevating seat and the tenth translation seat 641, such that the seventh elevating driving mechanism 642 can drive the seventh elevating seat to ascend or descend relative to the tenth translation seat 641. The fifth pipetting mechanism 64 is arranged on the seventh lifting seat so that the fifth pipetting mechanism 64 can move together with the seventh lifting seat. Specifically, the seventh elevating driving mechanism 642 is mounted on the tenth translation seat 641 and is drivingly connected to the seventh elevating seat. In this way, the seventh lifting seat can be driven to move up and down by the seventh lifting driving mechanism 642, so as to drive the fifth pipetting mechanism 64 to move up and down, so that the fifth pipetting mechanism 64 can aspirate the nucleic acid extracting solution in the orifice plate consumable B2 or inject the aspirated nucleic acid extracting solution into the reaction tube B3.

Further, the seventh elevation driving mechanism 642 includes a seventh screw, a seventh elevation driving member, and a seventh screw nut. The seventh screw rod is rotatably connected to the tenth translation seat 641 around its own axis, and the axial direction of the seventh screw rod is the vertical direction. The seventh lifting driving piece is mounted on the tenth translation seat 641 and is in driving connection with the seventh screw rod so as to drive the seventh screw rod to rotate around the axis of the seventh screw rod. The seventh screw nut is connected to the seventh screw rod in a threaded manner and is fixedly connected with the seventh lifting seat. In this way, when the fifth pipetting mechanism 64 needs to perform the lifting movement, the seventh lifting driving member drives the seventh screw rod to rotate, so as to drive the seventh screw rod nut and the seventh screw rod to move relatively in the axial direction (i.e. the vertical direction) of the seventh screw rod, and thus drive the seventh lifting seat and the fifth pipetting mechanism 64 on the seventh lifting seat to move (i.e. lift or descend) relatively to the tenth translation seat 641 in the vertical direction. Alternatively, the seventh lift drive may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the seventh lifting seat and the tenth translation seat 641, so that the lifting movement of the seventh lifting seat relative to the tenth translation seat 641 is more stable and reliable.

Referring to fig. 6, in an embodiment, the third transferring device 60 further includes an eleventh translation driving mechanism 654, an eleventh translation seat 651, and a third grabbing mechanism 65. The eleventh translation driving mechanism 654 is mounted on the sixth translation seat 67, and the eleventh translation seat 651 is movably connected to the sixth translation seat 67 along the second horizontal direction Y and is in driving connection with the eleventh translation driving mechanism 654, so that the eleventh translation driving mechanism 654 can drive the eleventh translation seat 651 to move along the second horizontal direction Y relative to the sixth translation seat 67. The third grabbing mechanism 65 is disposed on the eleventh translation seat 651, so that the third grabbing mechanism 65 can move along the third horizontal direction and the second horizontal direction Y along the eleventh translation seat 651, and further the third grabbing mechanism 65 can transfer the hole plate consumable B2 on the bearing base to the discarding position of the reaction area 13.

Further, the eleventh translational drive mechanism 654 includes an eleventh translational drive, an eleventh drive pulley 6541, an eleventh driven pulley 6542, and an eleventh belt 6543. The eleventh translation drive is mounted on the sixth translation seat 67 and the eleventh drive wheel 6541 is mounted on the output shaft of the eleventh translation drive such that the eleventh translation drive is capable of driving the eleventh drive wheel 6541 to rotate. The eleventh driven pulley 6542 is mounted on the sixth translation seat 67 and is spaced apart from the eleventh driving pulley 6541 along the second horizontal direction Y. The eleventh transmission belt 6543 is sleeved between the eleventh driving wheel 6541 and the eleventh driven wheel 6542, so that the eleventh transmission belt 6543 sequentially moves forward between the eleventh driving wheel 6541 and the eleventh driven wheel 6542 under the drive of the eleventh driving wheel 6541. The eleventh driving belt 6543 is fixedly connected to the eleventh translation holder 651 such that the eleventh driving belt 6543 can drive the eleventh translation holder 651 to move along the second horizontal direction Y. Alternatively, the eleventh translational drive may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the eleventh translation seat 651 and the sixth translation seat 67, so that the movement of the eleventh translation seat 651 along the second horizontal direction Y relative to the sixth translation seat 67 is more stable and reliable.

Further, the third transfer device 60 further includes an eighth elevating driving mechanism 652 and an eighth elevating base 653. The eighth elevating seat 653 is elevatably coupled to the eleventh translation seat 651. The eighth elevating driving mechanism 652 is drivingly connected between the eighth elevating seat 653 and the eleventh translation seat 651, such that the eighth elevating driving mechanism 652 can drive the eighth elevating seat 653 to ascend or descend with respect to the eleventh translation seat 651. The third grasping mechanism 65 is provided on the eighth elevating base 653 such that the third grasping mechanism 65 can move together with the eighth elevating base 653. Specifically, the eighth elevating drive mechanism 652 is mounted on the eleventh translation seat 651 and is drivingly connected to the eighth elevating seat 653. Thus, the eighth lifting seat 653 can be driven to move up and down by the eighth lifting driving mechanism 652, so as to drive the third grabbing mechanism 65 to move up and down, so that the third grabbing mechanism 65 grabs the orifice plate consumable B2, and transfers the orifice plate consumable B2 to the discarding position of the waste orifice plate in the reaction zone 13. Alternatively, the third gripping mechanism 65 may be a robot.

Further, the eighth elevating driving mechanism 652 includes an eighth screw rod, an eighth elevating driving member, and an eighth screw nut. The eighth screw rod is rotatably connected to the eleventh translation seat 651 around its own axis, and the axial direction of the eighth screw rod is in the vertical direction. The eighth lifting driving piece is installed on the eleventh translation seat 651 and is in driving connection with the eighth screw rod so as to drive the eighth screw rod to rotate around the axis of the eighth screw rod. The eighth screw nut is connected to the eighth screw rod through a thread, and is fixedly connected with the eighth lifting seat 653. Thus, when the third grabbing mechanism 65 needs to perform the lifting motion, the eighth lifting driving member drives the eighth screw rod to rotate, so as to drive the eighth screw rod nut and the eighth screw rod to move relatively along the axial direction (i.e. the vertical direction) of the eighth screw rod, and drive the eighth lifting seat 653 and the third grabbing mechanism 65 on the eighth lifting seat 653 to move (i.e. rise or fall) relatively to the eleventh translation seat 651 along the vertical direction. Alternatively, the eighth elevating driving member may employ a motor.

Optionally, a guiding structure, such as a sliding rail and a sliding block, may be disposed between the eighth lifting seat 653 and the eleventh translation seat 651, so that the lifting movement of the eighth lifting seat 653 relative to the eleventh translation seat 651 is more stable and reliable.

The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.

Claims

1. A nucleic acid extraction apparatus, comprising:

a mounting frame (10) having a sample region (11), an orifice region (12) and a reaction region (13) sequentially arranged at intervals along a first horizontal direction (X); the sample area (11) is used for storing a sample tube (B1), the pore plate area (12) is used for storing a pore plate consumable (B2), and the reaction area (13) is used for storing a reaction tube (B3);

A cover opening/closing device (20) provided between the sample area (11) and the well plate area (12);

a first transfer device (30) disposed in the sample region (11) and configured to transfer the sample tube (B1) in the sample region (11) to the cap opening/closing device (20), so that the cap opening/closing device (20) opens/closes the sample tube (B1);

the second transfer device (40) is arranged in the orifice plate area (12) and is used for transferring the sample in the uncapped sample tube (B1) into an orifice plate consumable (B2) of the orifice plate area (12);

the extraction device (50) is arranged between the orifice plate area (12) and the reaction area (13), and the second transfer device (40) is also used for transferring the orifice plate consumable (B2) of the orifice plate area (12) to the extraction device (50) so that the extraction device (50) can extract nucleic acid from a sample in the orifice plate consumable (B2) and obtain a nucleic acid extract; a kind of electronic device with high-pressure air-conditioning system

And the third transfer device (60) is arranged in the reaction zone (13) and is used for transferring the nucleic acid extracting solution in the orifice plate consumable (B2) on the extraction device (50) into a reaction tube (B3) of the reaction zone (13).

2. The nucleic acid extraction apparatus according to claim 1, characterized in that the opening and closing cover device (20) comprises a clamping base (21) and a rotating jaw (22) provided on the mounting frame (10), the clamping base (21) being movable in the first horizontal direction (X) with respect to the mounting frame (10) and being routed in sequence through a first position (A1), a second position (A2) and a third position (A3); the first position (A1) is located on one side of the second position (A2) close to the sample area (11), the third position (A3) is located on one side of the second position (A2) close to the orifice area (12), and the rotary clamping jaw (22) is located above the second position (A2);

-said gripping base (21) being adapted to receive a sample tube (B1) transferred by said first transfer means (30) when moved to said first position (A1); when the clamping base (21) moves to the second position (A2), the rotary clamping jaw (22) clamps a tube cover of the sample tube (B1) and performs screwing; when the clamping base (21) moves to the third position (A3), the sample in the sample tube (B1) on the clamping base (21) is transferred by the second transfer device (40).

3. The nucleic acid extraction apparatus according to claim 1, characterized in that the extraction device (50) comprises a carrying base and an extraction mechanism provided on the mounting frame (10), the carrying base being movable relative to the mounting frame (10) along the first horizontal direction (X) and being routed in sequence through a fourth position (A4), a fifth position (A5) and a sixth position (A6); the fourth position (A4) is positioned on one side of the fifth position (A5) close to the pore plate region (12), the sixth position (A6) is positioned on one side of the fifth position (A5) close to the reaction region (13), and the extraction mechanism is positioned above the fifth position (A5);

when the bearing base moves to the fourth position (A4), the bearing base is used for bearing the orifice plate consumable (B2) transferred by the second transfer device (40); when the bearing base moves to the fifth position (A5), the extraction mechanism extracts nucleic acid from a sample in the orifice plate consumable (B2) on the bearing base; when the bearing base moves to the sixth position (A6), the nucleic acid extracting solution in the orifice plate consumable (B2) on the bearing base is transferred by the third transferring device (60).

4. The nucleic acid extraction apparatus according to claim 1, characterized in that the first translation device (30) comprises a first translation driving mechanism (31), a first translation seat (32), a second translation driving mechanism (33), a second translation seat (34) and a first grasping mechanism (35);

the first translation driving mechanism (31) is mounted on the mounting frame (10), and the first translation seat (32) is movably connected to the mounting frame (10) along a second horizontal direction (Y) and is in driving connection with the first translation driving mechanism (31);

the second translation driving mechanism (33) is mounted on the first translation seat (32), and the second translation seat (34) is movably connected to the first translation seat (32) along a third horizontal direction and is in driving connection with the second translation driving mechanism (33); the first grabbing mechanism (35) is mounted on the second translation seat (34);

wherein the second horizontal direction (Y) intersects the third horizontal direction.

5. The nucleic acid isolation apparatus according to claim 4, characterized in that the first transfer device (30) further comprises a first lifting drive mechanism (37) and a first lifting seat (36), the first lifting seat (36) being liftably connected to the second translation seat (34), the first lifting drive mechanism (37) being mounted on the second translation seat (34) and being in driving connection with the first lifting seat (36);

The first grabbing mechanism (35) is arranged on the first lifting seat (36).

6. The nucleic acid isolation apparatus according to claim 1, characterized in that the second transfer device (40) comprises a third translational drive mechanism (41), a third translational seat (42), a fourth translational drive mechanism (43), a fourth translational seat (44), and a first pipetting mechanism (46);

the third translation driving mechanism (41) is mounted on the mounting frame (10), and the third translation seat (42) is movably connected to the mounting frame (10) along a third horizontal direction and is in driving connection with the third translation driving mechanism (41); the fourth translation driving mechanism (43) is mounted on the third translation seat (42), and the fourth translation seat (44) is movably connected to the third translation seat (42) along the second horizontal direction (Y) and is in driving connection with the fourth translation driving mechanism (43); the first pipetting mechanism (46) is arranged on the fourth translation seat (44);

7. The nucleic acid isolation apparatus according to claim 6, characterized in that the second transfer device (40) further comprises a second elevating drive mechanism (45) and a second elevating seat (48), the second elevating seat (48) being liftably connected to the fourth elevating seat (44), the second elevating drive mechanism (45) being mounted on the fourth elevating seat (44) and being drivingly connected to the second elevating seat (48);

The first pipetting mechanism (46) is arranged on the second lifting seat (48).

8. The nucleic acid extraction apparatus of claim 6, characterized in that the second transfer device (40) further comprises a fifth translational drive mechanism (472), a fifth translational seat (474), and a second grasping mechanism (47);

the fifth translation driving mechanism (472) is mounted on the third translation seat (42), and the fifth translation seat (474) is movably connected to the third translation seat (42) along the second horizontal direction (Y) and is in driving connection with the fifth translation driving mechanism (472);

the second grabbing mechanism (47) is arranged on the fifth translation seat (474).

9. The nucleic acid isolation apparatus of claim 8, wherein the second transfer device (40) further comprises a third elevation drive mechanism (473) and a third elevation seat (471), the third elevation seat (471) being liftably connected to the fifth translation seat (474), the third elevation drive mechanism (473) being mounted to the fifth translation seat (474) and being drivingly connected to the third elevation seat (471);

the second grabbing mechanism (47) is arranged on the third lifting seat (471).

10. The nucleic acid isolation apparatus of claim 1, wherein the third transfer device (60) further comprises a sixth translational drive mechanism (66), a sixth translational seat (67), a seventh translational drive mechanism (681), a seventh translational seat (611), and a second pipetting mechanism (61);

The sixth translation driving mechanism (66) is mounted on the mounting frame (10), and the sixth translation seat (67) is movably connected to the mounting frame (10) along a third horizontal direction and is in driving connection with the sixth translation driving mechanism (66); the seventh translation driving mechanism (681) is mounted on the sixth translation seat (67), and the seventh translation seat (611) is movably connected to the sixth translation seat (67) along a second horizontal direction (Y) and is in driving connection with the seventh translation driving mechanism (681); the second pipetting mechanism (61) is arranged on the seventh translation seat (611);

11. The nucleic acid isolation apparatus of claim 10, wherein the third transfer device (60) further comprises an eighth translation seat (621), an eighth translation drive mechanism (682), and a third pipetting mechanism (62);

a first sliding rail (a 1) extending lengthwise along the second horizontal direction (Y) is arranged on the sixth translation seat (67), a first sliding block (a 2) is arranged on the seventh translation seat (611), a second sliding block is arranged on the eighth translation seat (621), and the first sliding block (a 2) and the second sliding block are in sliding fit with the first sliding rail (a 1);

The eighth translation driving mechanism (682) is mounted on the sixth translation seat (67) and is in driving connection with the eighth translation seat (621); the third pipetting mechanism (62) is disposed on the eighth translation seat (621).

12. The nucleic acid extraction apparatus of claim 10, characterized in that the third transfer device (60) further comprises an eleventh translational drive mechanism (654), an eleventh translational seat (651), and a third grasping mechanism (65);

the eleventh translation driving mechanism (654) is mounted on the sixth translation seat (67), the eleventh translation seat (651) is movably connected to the sixth translation seat (67) along the second horizontal direction (Y), and is in driving connection with the eleventh translation driving mechanism (654), and the third grabbing mechanism (65) is disposed on the eleventh translation seat (651).