CN116908276A - Sampling device for three-dimensional biological mass spectrum imaging probe - Google Patents
Sampling device for three-dimensional biological mass spectrum imaging probe Download PDFInfo
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- CN116908276A CN116908276A CN202310912277.0A CN202310912277A CN116908276A CN 116908276 A CN116908276 A CN 116908276A CN 202310912277 A CN202310912277 A CN 202310912277A CN 116908276 A CN116908276 A CN 116908276A
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- 238000005070 sampling Methods 0.000 title claims abstract description 99
- 239000000523 sample Substances 0.000 title claims abstract description 84
- 238000003384 imaging method Methods 0.000 title claims abstract description 34
- 238000001819 mass spectrum Methods 0.000 title claims abstract description 19
- 230000007246 mechanism Effects 0.000 claims abstract description 107
- 239000011521 glass Substances 0.000 claims abstract description 19
- 239000003960 organic solvent Substances 0.000 claims abstract description 8
- 230000033001 locomotion Effects 0.000 claims description 245
- 239000010902 straw Substances 0.000 claims description 50
- 239000007921 spray Substances 0.000 claims description 22
- 238000004949 mass spectrometry Methods 0.000 claims description 12
- 238000003825 pressing Methods 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 238000009966 trimming Methods 0.000 claims 1
- 238000004458 analytical method Methods 0.000 abstract description 15
- 150000001875 compounds Chemical class 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 2
- 239000000090 biomarker Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000036267 drug metabolism Effects 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000007639 printing Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 238000001871 ion mobility spectroscopy Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
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- 239000002904 solvent Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention discloses a three-dimensional biological mass spectrum imaging probe sampling device, which comprises an object carrying platform for placing a glass slide for carrying a sample, wherein a needle seat is arranged above the object carrying platform, a sampling needle with the lower end extending out of the needle seat and a conductive touch ball connected with the sampling needle are connected to the needle seat, the bottom of the object carrying platform is connected with a platform moving mechanism for driving the object carrying platform to move, a vertically arranged mounting plate is arranged on the platform moving mechanism, a conductive sheet is arranged above the conductive touch ball on the mounting plate, a needle seat vertical moving mechanism for driving the needle seat to vertically move so that the sampling needle is inserted into the glass slide sample or the conductive touch ball is contacted with the conductive sheet, and a nozzle assembly with the output end close to the sampling needle and ejecting an organic solvent to dissolve the sample is arranged above the platform moving mechanism, and a suction pipe with the suction end close to the sampling needle for sucking the ionized dissolved sample is arranged above the object carrying platform, so that the sample analysis point of the sample is rapidly positioned and the accurate depth sampling function of the compound sample at the corresponding point is realized.
Description
[ technical field ]
The invention relates to a sampling device of a three-dimensional biological mass spectrum imaging probe.
[ background Art ]
Imaging analysis of biological tissue in biological and medical research is a very important task to obtain imaging information of a sample. However, the conventional imaging techniques such as CT can obtain the change position of the tissue structure, but the type and content of the chemical components such as the metabolic products, proteins, lipids and polypeptides at the change position cannot be known. The mass spectrum imaging technology Imaging Mass Spectrometry and the IMS not only have the advantages of rapidness, high precision, high sensitivity and the like of the common mass spectrum technology, but also can obtain the spatial distribution situation of various compounds in the original sample, and become an attractive technology in the field of biological tissue imaging.
Biological samples are a complex system, and obtaining the distribution of different types of compounds in biological samples is of great importance for studying the physiology of organisms, drug metabolism, the discovery of biomarkers and the monitoring of compounds. Therefore, mass spectrometry imaging technology is not only required to study biological macromolecules in tissue samples, but also required to analyze small molecular compounds in the tissue samples at the same time, and can rapidly and accurately provide accurate information of spatial distribution of the molecules in the tissue, so that analytes can be detected and relatively quantified in an initial form so as to meet analysis requirements of related fields such as biological physiology, drug metabolism, biomarker discovery and the like. These analysis requirements place higher demands on traditional mass spectrometry imaging techniques, particularly ionization techniques for mass spectrometry imaging and rapid positioning of analysis sites and sampling modes of corresponding point compounds, where ionization structures are complex, sample analysis point positioning is inaccurate, and it is difficult to accurately sample a set sampling depth.
[ summary of the invention ]
The invention overcomes the defects of the prior art, provides the three-dimensional biological mass spectrum imaging probe sampling device, simplifies the ionization structure and realizes the functions of rapid positioning and accurate depth sampling of a sample analysis point.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a three-dimensional biological mass spectrum imaging probe sampling device which is characterized in that: the device comprises a carrying platform for placing a glass slide for loading samples, wherein a needle seat is arranged above the carrying platform, a sampling needle with the lower end extending out of the needle seat and a conductive contact ball connected with the sampling needle are connected to the needle seat, a platform moving mechanism for driving the carrying platform to move transversely and longitudinally is connected to the bottom of the carrying platform, a vertically arranged mounting plate is arranged on the platform moving mechanism, a conductive sheet is arranged above the conductive contact ball on the mounting plate, a needle seat vertical moving mechanism for driving the needle seat to move vertically to enable the sampling needle to be inserted into the glass slide sample or enable the conductive contact ball to contact with the conductive sheet is arranged on the mounting plate, and a nozzle assembly with an output end close to the sampling needle and spraying organic solvent to dissolve the sample is arranged above the platform moving mechanism, a suction pipe with a suction end close to the sampling needle for sucking ionized dissolved samples is arranged above the carrying platform and is fed into a mass spectrometer, and a contact ball stop for limiting the maximum upward movement position of the conductive contact ball is arranged above the conductive sheet on the mounting plate.
The three-dimensional biological mass spectrum imaging probe sampling device is characterized in that: the needle seat vertical movement mechanism comprises a needle seat movement mechanism base arranged on the mounting plate, a vertically arranged needle seat vertical movement sliding rail is arranged in the needle seat movement mechanism base, a needle seat vertical movement sliding block is arranged on the needle seat vertical movement sliding rail, a needle seat vertical movement panel is connected on the needle seat vertical movement sliding block, a needle seat vertical movement motor is arranged on the needle seat movement mechanism base, a needle seat vertical movement ball screw is connected between a needle seat vertical movement motor rotating shaft and the needle seat vertical movement panel, and the needle seat is arranged on the needle seat vertical movement panel.
The three-dimensional biological mass spectrum imaging probe sampling device is characterized in that: the inner side surface of the needle seat vertical movement panel is provided with a needle seat vertical movement panel lug, the base of the needle seat movement mechanism is provided with needle seat vertical movement sensors which are positioned on two sides of the movement direction of the needle seat vertical movement panel lug and are respectively provided with a needle seat vertical movement sensor for detecting the needle seat vertical movement panel lug so as to limit the movement position of the needle seat vertical movement panel lug.
The three-dimensional biological mass spectrum imaging probe sampling device is characterized in that: the platform motion is last to be equipped with the mounting panel triaxial moving mechanism of regulation mounting panel position, and mounting panel triaxial moving mechanism is including setting up the mounting panel moving assembly base on platform motion, is equipped with on the mounting panel moving assembly base by supreme mounting panel longitudinal movement subassembly, the vertical moving assembly of mounting panel and the mounting panel transverse movement subassembly of being equipped with in proper order down, and the mounting panel is connected on the mounting panel transverse movement subassembly mobile terminal.
The three-dimensional biological mass spectrum imaging probe sampling device is characterized in that: the mounting plate is provided with a spray head moving mechanism for adjusting the position of the spray head assembly, the spray head moving mechanism comprises a spray head longitudinal moving assembly and a spray head transverse moving assembly which are sequentially arranged on the mounting plate from bottom to top, and the spray head assembly is connected to the moving end of the spray head transverse moving assembly.
The three-dimensional biological mass spectrum imaging probe sampling device is characterized in that: be equipped with the vertical motion subassembly of straw of adjusting the vertical position of straw on the platform motion, be equipped with the straw lateral adjustment mechanism of adjusting the straw and absorb end lateral position on the vertical motion subassembly mobile end of straw, straw lateral adjustment mechanism is including connecting the straw locating plate on the vertical motion subassembly mobile end of straw, the straw transversely passes the straw locating plate, lie in the straw locating plate rear side on the straw and be equipped with the constant pressure spring seat, the cover is equipped with the spring with constant pressure spring seat, straw locating plate contact respectively on the straw, lie in the straw locating plate front side on the straw and be equipped with the dynamic pressure spring seat that supports the pressure straw locating plate.
The three-dimensional biological mass spectrum imaging probe sampling device is characterized in that: the platform motion mechanism comprises a platform motion mechanism base, a platform transverse sliding rail which is transversely arranged is arranged on the bottom surface of the inner side of the platform motion mechanism base, a platform transverse sliding block is arranged on the platform transverse sliding rail, a platform transverse motion panel is connected to the platform transverse sliding block, a platform transverse motion motor is arranged on the bottom surface of the inner side of the platform motion mechanism base, a platform transverse motion ball screw is connected between a platform transverse motion motor rotating shaft and the platform transverse motion panel, a platform longitudinal sliding rail which is longitudinally arranged is arranged on the top surface of the platform transverse motion panel, a platform longitudinal sliding block is arranged on the platform longitudinal sliding rail, a platform longitudinal motion panel connected with the bottom of the carrying platform is arranged on the platform longitudinal sliding block, a platform longitudinal motion motor is arranged on the top surface of the platform transverse motion panel, and a platform longitudinal motion ball screw is connected between the platform longitudinal motion motor rotating shaft and the platform longitudinal motion panel.
The three-dimensional biological mass spectrum imaging probe sampling device is characterized in that: the bottom of the platform transverse movement panel is provided with a platform transverse movement panel lug, and two sides of the bottom surface of the inner side of the platform movement mechanism base, which are positioned in the movement direction of the platform transverse movement panel lug, are respectively provided with a platform transverse movement sensor for detecting the limiting transverse movement position of the platform transverse movement panel lug; the bottom of the platform longitudinal movement panel is provided with a platform longitudinal movement panel lug, and two sides of the bottom surface of the inner side of the platform movement mechanism base, which are positioned in the movement direction of the platform longitudinal movement panel lug, are respectively provided with a platform longitudinal movement sensor for detecting the limiting longitudinal movement position of the platform longitudinal movement panel lug.
The three-dimensional biological mass spectrum imaging probe sampling device is characterized in that: the mass spectrometer sampling interface is arranged on the connecting plate, the platform vertical fine adjustment device is used for adjusting the vertical position of the platform moving mechanism and comprises a platform vertical moving sliding rail, a platform vertical moving sliding block and a fine adjustment assembly, the platform vertical moving sliding rail is arranged on the side surface of the platform moving mechanism and is vertically arranged, the platform vertical moving sliding block is arranged on the connecting plate and slides on the platform vertical moving sliding rail, and the fine adjustment assembly is arranged between the platform moving mechanism and the connecting plate.
The three-dimensional biological mass spectrum imaging probe sampling device is characterized in that: the bottom of the platform movement mechanism is provided with an elastic supporting rod for adjusting the height of the platform movement mechanism, and the elastic supporting rod comprises a lower support, an upper support connected with the bottom of the platform movement mechanism and a lifting screw rod arranged between the lower support and the upper support and used for adjusting the height of the upper support.
The beneficial effects of the invention are as follows:
according to the invention, the platform moving mechanism is arranged below the sampling needle, the object carrying platform and the glass slide are driven to do transverse and longitudinal two-dimensional movement to rapidly position the analysis point of the sample, the needle seat and the sampling needle are driven to move downwards by the needle seat vertical moving mechanism to sample the set depth of the analysis point of the sample, the conductive contact ball is enabled to be contacted with the conductive sheet to be electrified by upward movement after sampling, the nozzle component ejects an organic solvent to the sampling needle to sample the sample to dissolve the sample and sends the sample into the mass spectrometer through the suction pipe after high-voltage ionization, so that the ionization structure is simplified, and the rapid positioning of the analysis point of the sample and the accurate depth sampling function of the compound sample at the corresponding point are realized.
[ description of the drawings ]
FIG. 1 is a schematic diagram of one embodiment of the present invention;
FIG. 2 is a second schematic diagram of the structure of the present invention;
FIG. 3 is a third schematic diagram of the structure of the present invention;
FIG. 4 is an exploded view of the present invention;
fig. 5 is a schematic view of one of the vertical motion mechanism and the nozzle moving mechanism of the needle stand of the present invention;
FIG. 6 is a second schematic view of the vertical motion mechanism of the needle stand and the nozzle moving mechanism of the present invention;
fig. 7 is an exploded view of the vertical movement mechanism of the needle holder of the present invention;
FIG. 8 is an exploded view of the platform motion mechanism of the present invention;
FIG. 9 is a second exploded view of the platform motion mechanism of the present invention.
Detailed description of the preferred embodiments
The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.
It should be noted that all directional indicators (such as up, down, left, right, front, and rear …) in the embodiments of the present invention are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly. Furthermore, the description of "preferred," "less preferred," and the like, herein is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "preferred", "less preferred" may include at least one such feature, either explicitly or implicitly.
As shown in fig. 1-9, a three-dimensional biological mass spectrum imaging probe sampling device comprises a carrying platform 2 for placing a glass slide 1 for loading a sample, a needle seat 3 is arranged above the carrying platform 2, a sampling needle 4 with the lower end extending out of the needle seat 3 and a conductive contact ball 5 connected with the sampling needle 4 are connected to the needle seat 3, a platform moving mechanism 6 for driving the carrying platform 2 to move transversely and longitudinally is connected to the bottom of the carrying platform 2, a vertically arranged mounting plate 7 is arranged on the platform moving mechanism 6, a conductive sheet 8 is arranged above the conductive contact ball 5 on the mounting plate 7, a needle seat vertical moving mechanism 9 for driving the needle seat 3 to move vertically to enable the sampling needle 4 to be inserted into the sample of the glass slide 1 or enable the conductive contact ball 5 to be in contact with the conductive sheet 8 is arranged on the mounting plate 7, a nozzle assembly 10 with the output end close to the sampling needle 4 and spraying an organic solvent to dissolve the sample is arranged above the carrying platform moving mechanism 6, and a suction pipe 11 with a suction end close to the sampling needle 4 to suck ionized dissolved sample and send the dissolved sample into a mass spectrometer is arranged above the carrying platform 2. Wherein, be equipped with the glass safety cover in fig. 1, but the sampling condition is observed to printing opacity glass safety cover, and the glass safety cover forms the test chamber, and the sampling process is gone on in the test chamber.
In actual use, a solid sample is placed on a glass slide 1, then the sample is communicated with the glass slide 1 and placed on a carrying platform 2, and a platform motion mechanism 6 drives the carrying platform 2 to move transversely and longitudinally in two dimensions according to the set sample analysis points, so that the sample analysis points of the glass slide 1 are moved to the position below a sampling needle 4, and the functions of rapidly positioning the sample analysis points and performing multi-point scanning sampling on the solid sample are realized; then according to the set sample analysis point sampling depth data, the needle seat vertical movement mechanism 9 drives the needle seat 3 to move downwards, so as to drive the sampling needle 4 to move downwards to the set sample sampling depth and sample, thereby realizing the accurate sample depth sampling function, after the sampling needle 4 samples, the needle seat vertical movement mechanism 9 drives the needle seat 3 and the sampling needle 4 to move upwards, so that the conductive contact ball 5 is in contact with the conductive sheet 8 for electrifying, the spray head assembly 10 sprays an organic solvent to the sample sampling position of the sampling needle 4 to dissolve the sample, and after ionization, the solvent sample substance is sent into the mass spectrometer through the suction tube 11 to be tested and recorded, and data statistics, 3D display and printing are performed.
As shown in fig. 4-7, the vertical motion mechanism 9 of the needle stand comprises a seat motion mechanism base 91 installed on the mounting plate 7, a vertically arranged seat vertical motion sliding rail 92 is arranged in the seat motion mechanism base 91, a seat vertical motion sliding block 93 is arranged on the seat vertical motion sliding rail 92, a seat vertical motion panel 94 is connected on the seat vertical motion sliding block 93, a seat vertical motion motor 95 is arranged on the seat motion mechanism base 91, a seat vertical motion ball screw 96 is connected between the rotating shaft of the seat vertical motion motor 95 and the seat vertical motion panel 94, and the needle stand 3 is installed on the seat vertical motion panel 94. When the needle seat 3 is moved up and down, the needle seat vertical movement motor 95 drives the needle seat vertical movement sliding block 93 to move up and down through the needle seat vertical movement ball screw 96, so as to drive the needle seat vertical movement panel 94 and the needle seat 3 to move up and down, realize accurate sample depth sampling and enable the conductive contact ball 5 to be in contact with the conductive sheet 8, and further enable the sampling needle 4 to be electrified.
As shown in fig. 5-6, the inner side of the needle seat vertical movement panel 94 is provided with a needle seat vertical movement panel projection 97, and the needle seat movement mechanism base 91 is provided with a needle seat vertical movement sensor 98 which is positioned on both sides of the movement direction of the needle seat vertical movement panel projection 97 and is used for detecting the needle seat vertical movement panel projection 97 so as to limit the movement position of the needle seat vertical movement panel projection 97. When the boss 97 of the vertical movement panel of the needle seat moves up and down to touch the vertical movement sensor 98 of the needle seat, the vertical movement motor 95 of the needle seat stops working, thereby limiting the highest and lowest movement positions of the needle seat 3; as shown in fig. 6, a contact ball stop 12 for limiting the maximum upward movement position of the conductive contact ball 5 is arranged above the conductive sheet 8 on the mounting plate 7, so that the conductive contact ball 5 is contacted with the conductive sheet 8 for power supply.
As shown in fig. 3 and 6, the platform motion mechanism 6 is provided with a mounting plate triaxial moving mechanism 13 for adjusting the position of the mounting plate 7, the mounting plate triaxial moving mechanism 13 comprises a mounting plate moving assembly base 131 arranged on the platform motion mechanism 6, the mounting plate moving assembly base 131 is provided with a mounting plate longitudinal moving assembly 132, a mounting plate vertical moving assembly 133 and a mounting plate transverse moving assembly 134 which are sequentially arranged from bottom to top, and the mounting plate 7 is connected to the moving end of the mounting plate transverse moving assembly 134. The three-dimensional position of the mounting plate 7 is adjusted through the mounting plate longitudinal moving assembly 132, the mounting plate vertical moving assembly 133 and the mounting plate transverse moving assembly 134, so that the relative initial position between the sampling needle 4 and the glass slide 1 is better set, and the sampling needle 4 can accurately position the sampling point and accurately sample the set depth under the movement of the platform moving mechanism 6 and the needle seat vertical moving mechanism 9.
As shown in fig. 5-6, the mounting plate 7 is provided with a nozzle moving mechanism 14 for adjusting the position of the nozzle assembly 10, the nozzle moving mechanism 14 comprises a nozzle longitudinal moving assembly 141 and a nozzle transverse moving assembly 142 which are sequentially arranged on the mounting plate 7 from bottom to top, and the nozzle assembly 10 is connected to the moving end of the nozzle transverse moving assembly 142. The relative positions of the spraying end of the spray head assembly 10 and the sampling needle 4 are adjusted through the spray head longitudinal moving assembly 141 and the spray head transverse moving assembly 142, so that the organic solvent sprayed by the spray head assembly 10 is accurately sprayed to the sampling position of the sampling needle 4 in the conductive state.
As shown in fig. 2 and 4, the platform moving mechanism 6 is provided with a straw vertical moving assembly 15 for adjusting the vertical position of the straw 11, and the height of the suction end of the straw 11 is similar to that of the sampling position of the sampling needle 4 in a conductive state by adjusting the vertical position of the straw 11; a suction pipe transverse adjusting mechanism 16 for adjusting the transverse position of the suction end of the suction pipe 11 is arranged at the moving end of the suction pipe vertical movement assembly 15 and is used for adjusting the distance between the suction end of the suction pipe 11 and the sampling position of the sampling needle 4 so that the suction pipe 11 sucks ionized sample dissolved components; as shown in fig. 4, the straw transverse adjusting mechanism 16 comprises a straw positioning plate 161 connected to the moving end of the straw vertical motion assembly 15, the straw 11 transversely passes through the straw positioning plate 161, a fixed pressure spring seat 162 is arranged on the straw 11 and located at the rear side of the straw positioning plate 161, springs 163 respectively contacting with the fixed pressure spring seat 162 and the straw positioning plate 161 are sleeved on the straw 11, and a pressure spring seat 164 for pressing the straw positioning plate 161 is arranged on the straw 11 and located at the front side of the straw positioning plate 161. During adjustment, the compression length of the spring 163 arranged between the fixed pressure spring seat 162 and the suction tube positioning plate 161 is adjusted by moving the fixed pressure spring seat 162, so that the distance between the suction end of the suction tube 11 and the sampling position of the sampling needle 4 is adjusted.
As shown in fig. 8-9, the platform motion mechanism 6 includes a platform motion mechanism base 61, a platform transverse slide rail 62 transversely disposed is disposed on an inner bottom surface of the platform motion mechanism base 61, a platform transverse slide block 63 is disposed on the platform transverse slide rail 62, a platform transverse motion panel 64 is connected to the platform transverse slide block 63, a platform transverse motion motor 65 is disposed on an inner bottom surface of the platform motion mechanism base 61, a platform transverse motion ball screw 66 is connected between a rotating shaft of the platform transverse motion motor 65 and the platform transverse motion panel 64, a platform longitudinal slide rail 67 longitudinally disposed is disposed on a top surface of the platform transverse motion panel 64, a platform longitudinal slide block 68 is disposed on the platform longitudinal slide rail 67, a platform longitudinal motion panel 69 connected to a bottom of the carrying platform 2 is disposed on the platform longitudinal slide block 68, a platform longitudinal motion motor 610 is disposed on a top surface of the platform transverse motion panel 64, and a platform longitudinal motion ball screw 611 is connected between the rotating shaft of the platform longitudinal motion motor 610 and the platform longitudinal motion panel 69. In the sampling process, the platform transverse movement motor 65 drives the platform transverse movement ball screw 66 to rotate, so that the platform transverse movement panel 64 is driven to transversely move, and the platform longitudinal movement motor 610 drives the longitudinal movement ball screw 311 to rotate, so that the platform longitudinal movement panel 69 is driven to longitudinally move, and the transverse movement and the longitudinal two-dimensional movement of the carrying platform 2 are driven, and the set sample sampling point on the glass slide 1 moves to the lower part of the sampling needle 4, so that the function of rapidly and accurately positioning the sampling point is realized.
As shown in fig. 8-9, the bottom of the platform transverse movement panel 64 is provided with a platform transverse movement panel bump 612, and two sides of the inner bottom surface of the platform movement mechanism base 61, which are positioned in the movement direction of the platform transverse movement panel bump 612, are respectively provided with a platform transverse movement sensor 613 for detecting that the platform transverse movement panel bump 612 defines the transverse movement position; the bottom of the platform longitudinal movement panel 69 is provided with a platform longitudinal movement panel bump 614, the two sides of the inner bottom surface of the platform movement mechanism base 61, which are positioned in the movement direction of the platform longitudinal movement panel bump 614, are respectively provided with a platform longitudinal movement sensor 615 for detecting the longitudinal movement position defined by the platform longitudinal movement panel bump 614, the platform transverse movement sensor 613 and the platform longitudinal movement sensor 615 are arranged to define the maximum transverse position and the maximum longitudinal position, and the sampling needle 4 is ensured to sample samples in the effective sampling area of the glass slide 1.
As shown in fig. 8-9, the platform lateral movement sensor 613 is a micro switch, and the contact surface of the platform lateral movement panel bump 612, which is contacted with the pressing end of the micro switch, is a guiding inclined surface; the platform longitudinal motion sensor 615 is a micro switch, and a contact surface of the platform longitudinal motion panel bump 614, which is in contact with a pressing end of the micro switch, is a guiding inclined plane, so that the platform transverse motion panel bump 612 is convenient to touch the platform transverse motion sensor 613, and the platform longitudinal motion panel bump 614 is convenient to touch the platform longitudinal motion sensor 615.
As shown in fig. 1-4, the bottom of the platform motion mechanism 6 is provided with an elastic supporting rod 19 for adjusting the height of the platform motion mechanism 6, and the elastic supporting rod 19 comprises a lower support 191, an upper support 192 connected with the bottom of the platform motion mechanism 6, and a lifting screw 193 arranged between the lower support 191 and the upper support 192 for adjusting the height of the upper support 192. The height of the upper support 192 is adjusted by rotating the elevating screw 193, and the height of the stage moving mechanism 6 is adjusted, thereby adjusting the distance between the sample of the slide 1 and the sampling needle 4.
As shown in fig. 3-4, a connecting plate 17 is arranged between the platform moving mechanism 6 and the mass spectrometer, a sampling interface of the mass spectrometer is arranged on the connecting plate 17, a platform vertical fine adjustment device 18 for adjusting the vertical position of the platform moving mechanism 6 is arranged between the platform moving mechanism 6 and the connecting plate 17, and the platform vertical fine adjustment device 18 comprises a platform vertical moving sliding rail 181 which is arranged on the side surface of the platform moving mechanism 6 and is vertically arranged, a platform vertical moving sliding block 182 which is arranged on the connecting plate 17 and slides on the platform vertical moving sliding rail 181, and a fine adjustment assembly 183 which is arranged between the platform moving mechanism 6 and the connecting plate 17. The platform vertical movement sliding block 182 slides on the platform vertical movement sliding rail 181 through the rotation fine adjustment assembly 183, and the relative height between the platform movement mechanism 6 and the connecting plate 11 is adjusted, so that the initial height of a sample on the glass slide 1 relative to the sampling needle 4 is fine adjusted, and the accuracy of the sampling depth is improved.
The sample taking device is also provided with a main control electronic circuit box, wherein an X-Y double stepping motor in a control platform moving mechanism drives a sampling point of a slide sample to move below a sampling needle, a stepping motor of a needle seat vertical moving mechanism is controlled to move downwards to be inserted into the slide sample, a set sample depth is sampled, a conductive contact ball is enabled to be in contact with a blade to be electrified after the sampling, then a spray head assembly is controlled to spray an organic solvent to a sampling position of the sampling needle and control the sampling needle to electrify and ionize dissolved sample substances, and ionized sample components are sent into a mass spectrometer through a suction pipe 11 to be subjected to data processing analysis.
The foregoing description of the preferred embodiments of the present invention should not be construed as limiting the scope of the invention, but rather should be understood to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the following description and drawings or any application directly or indirectly to other relevant art(s).
Claims (10)
1. A three-dimensional biological mass spectrum imaging probe sampling device which is characterized in that: comprises a carrying platform (2) for placing a glass slide (1) for loading samples, a needle seat (3) is arranged above the carrying platform (2), a sampling needle (4) with the lower end extending out of the needle seat (3) and a conductive contact ball (5) connected with the sampling needle (4) are connected on the needle seat (3), a platform moving mechanism (6) for driving the carrying platform (2) to move transversely and longitudinally is connected at the bottom of the carrying platform (2), a vertically arranged mounting plate (7) is arranged on the platform moving mechanism (6), a conductive sheet (8) is arranged above the conductive contact ball (5) on the mounting plate (7), a needle seat vertical moving mechanism (9) for driving the needle seat (3) to move vertically so as to enable the sampling needle (4) to be inserted into a sample of the glass slide (1) or enable the conductive contact ball (5) to be contacted with the conductive sheet (8) is arranged on the mounting plate (7), a nozzle component (10) with the output end close to the sampling needle (4) and spraying an organic solvent to dissolve the sample is arranged above the carrying platform moving mechanism (6), a sample sucking and sucking sample (11) with the ionization suction tube (4) close to the sampling needle (4) on the upper end, a contact ball stop block (12) which limits the maximum upward movement position of the conductive contact ball (5) is arranged above the conductive sheet (8) on the mounting plate (7).
2. The three-dimensional biological mass spectrometry imaging probe sampling device according to claim 1, wherein: the needle seat vertical movement mechanism (9) comprises a needle seat movement mechanism base (91) arranged on the mounting plate (7), a vertically arranged needle seat vertical movement sliding rail (92) is arranged in the needle seat movement mechanism base (91), a needle seat vertical movement sliding block (93) is arranged on the needle seat vertical movement sliding rail (92), a needle seat vertical movement panel (94) is connected on the needle seat vertical movement sliding block (93), a needle seat vertical movement motor (95) is arranged on the needle seat movement mechanism base (91), a needle seat vertical movement ball screw (96) is connected between a rotating shaft of the needle seat vertical movement motor (95) and the needle seat vertical movement panel (94), and the needle seat (3) is arranged on the needle seat vertical movement panel (94).
3. A three-dimensional biological mass spectrometry imaging probe sampling device according to claim 2, wherein: the inner side surface of the needle seat vertical movement panel (94) is provided with a needle seat vertical movement panel lug (97), the needle seat movement mechanism base (91) is provided with needle seat vertical movement sensors (98) which are positioned on two sides of the movement direction of the needle seat vertical movement panel lug (97) and used for detecting the needle seat vertical movement panel lug (97) so as to limit the movement position of the needle seat vertical movement panel lug (97).
4. The three-dimensional biological mass spectrometry imaging probe sampling device according to claim 1, wherein: the platform motion mechanism (6) is provided with a mounting plate triaxial moving mechanism (13) for adjusting the position of the mounting plate (7), the mounting plate triaxial moving mechanism (13) comprises a mounting plate moving assembly base (131) arranged on the platform motion mechanism (6), the mounting plate moving assembly base (131) is provided with a mounting plate longitudinal moving assembly (132), a mounting plate vertical moving assembly (133) and a mounting plate transverse moving assembly (134) which are sequentially arranged from bottom to top, and the mounting plate (7) is connected to the moving end of the mounting plate transverse moving assembly (134).
5. The three-dimensional biological mass spectrometry imaging probe sampling device according to claim 1, wherein: the mounting plate (7) is provided with a spray head moving mechanism (14) for adjusting the position of the spray head assembly (10), the spray head moving mechanism (14) comprises a spray head longitudinal moving assembly (141) and a spray head transverse moving assembly (142) which are sequentially arranged on the mounting plate (7) from bottom to top, and the spray head assembly (10) is connected to the moving end of the spray head transverse moving assembly (142).
6. The three-dimensional biological mass spectrometry imaging probe sampling device according to claim 1, wherein: be equipped with straw vertical motion subassembly (15) of adjusting straw (11) vertical position on platform motion (6), be equipped with straw lateral adjustment mechanism (16) of adjusting straw (11) suction end lateral position on straw vertical motion subassembly (15) mobile end, straw lateral adjustment mechanism (16) are including connecting straw locating plate (161) on straw vertical motion subassembly (15) mobile end, straw (11) lateral pass straw locating plate (161), be located straw locating plate (161) rear side on straw (11) and be equipped with fixed pressure spring seat (162), the cover is equipped with spring (163) with fixed pressure spring seat (162), straw locating plate (161) contact respectively on straw (11), be located straw locating plate (161) front side on straw (11) and be equipped with dynamic pressure spring seat (164) of pressing straw locating plate (161).
7. The three-dimensional biological mass spectrometry imaging probe sampling device according to claim 1, wherein: the platform motion mechanism (6) comprises a platform motion mechanism base (61), a platform transverse sliding rail (62) which is transversely arranged is arranged on the inner bottom surface of the platform motion mechanism base (61), a platform transverse sliding block (63) is arranged on the platform transverse sliding rail (62), a platform transverse motion panel (64) is connected to the platform transverse sliding block (63), a platform transverse motion motor (65) is arranged on the inner bottom surface of the platform motion mechanism base (61), a platform transverse motion ball screw (66) is connected between a rotating shaft of the platform transverse motion motor (65) and the platform transverse motion panel (64), a platform longitudinal sliding rail (67) which is longitudinally arranged is arranged on the top surface of the platform transverse motion panel (64), a platform longitudinal sliding block (68) which is longitudinally arranged is arranged on the platform longitudinal sliding rail (67), a platform longitudinal motion panel (69) which is connected with the bottom of the carrying platform (2) is arranged on the platform transverse motion panel (64), and a platform longitudinal motion ball screw (611) is connected between the rotating shaft of the platform longitudinal motion motor (610) and the platform longitudinal motion panel (69).
8. The three-dimensional biological mass spectrometry imaging probe sampling device of claim 7, wherein: a platform transverse movement panel lug (612) is arranged at the bottom of the platform transverse movement panel (64), and platform transverse movement sensors (613) for detecting the limiting transverse movement position of the platform transverse movement panel lug (612) are respectively arranged at two sides of the inner bottom surface of the platform movement mechanism base (61) in the movement direction of the platform transverse movement panel lug (612); the bottom of the platform longitudinal movement panel (69) is provided with a platform longitudinal movement panel lug (614), and the two sides of the inner bottom surface of the platform movement mechanism base (61) in the movement direction of the platform longitudinal movement panel lug (614) are respectively provided with a platform longitudinal movement sensor (615) for detecting the longitudinal movement position limited by the platform longitudinal movement panel lug (614).
9. The three-dimensional biological mass spectrometry imaging probe sampling device according to claim 1, wherein: be equipped with connecting plate (17) between platform motion (6) and the mass spectrometer, mass spectrometer sampling interface sets up on connecting plate (17), be used for adjusting platform vertical micromatic setting (18) of platform motion (6) vertical position between platform motion (6) and connecting plate (17), platform vertical micromatic setting (18) are including setting up platform vertical motion slide rail (181) in platform motion (6) side and vertical setting, set up at connecting plate (17) gliding platform vertical motion slider (182) on platform vertical motion slide rail (181), and set up trimming module (183) between platform motion (6) and connecting plate (17).
10. The three-dimensional biological mass spectrometry imaging probe sampling device according to claim 1, wherein: the bottom of the platform movement mechanism (6) is provided with an elastic supporting rod (19) for adjusting the height of the platform movement mechanism (6), and the elastic supporting rod (19) comprises a lower support (191), an upper support (192) connected with the bottom of the platform movement mechanism (6) and a lifting screw (193) arranged between the lower support (191) and the upper support (192) for adjusting the height of the upper support (192).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310912277.0A CN116908276A (en) | 2023-07-24 | 2023-07-24 | Sampling device for three-dimensional biological mass spectrum imaging probe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310912277.0A CN116908276A (en) | 2023-07-24 | 2023-07-24 | Sampling device for three-dimensional biological mass spectrum imaging probe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116908276A true CN116908276A (en) | 2023-10-20 |
Family
ID=88367777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310912277.0A Withdrawn CN116908276A (en) | 2023-07-24 | 2023-07-24 | Sampling device for three-dimensional biological mass spectrum imaging probe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116908276A (en) |
-
2023
- 2023-07-24 CN CN202310912277.0A patent/CN116908276A/en not_active Withdrawn
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Application publication date: 20231020 |