CN116891890A - Artificial intelligent rapid pathogen detection method and application thereof in infectious disease diagnosis - Google Patents

Artificial intelligent rapid pathogen detection method and application thereof in infectious disease diagnosis Download PDF

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Publication number
CN116891890A
CN116891890A CN202310853492.8A CN202310853492A CN116891890A CN 116891890 A CN116891890 A CN 116891890A CN 202310853492 A CN202310853492 A CN 202310853492A CN 116891890 A CN116891890 A CN 116891890A
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detection method
sample
artificial intelligence
pathogen detection
rapid pathogen
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莫伟平
张泳仪
袁桂南
刘敏霞
梁柳如
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Dongguan Peoples Hospital
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Dongguan Peoples Hospital
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
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    • G16H50/00ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
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Abstract

The invention discloses an artificial intelligent rapid pathogen detection method, which comprises the following steps: step 1: collecting a sample; step 2: preparing a sample; step 3: detecting pathogens in the sample, and determining the types and the quantity of the pathogens; step 4: the detected data are analyzed and processed by using an artificial intelligence algorithm to generate a standardized diagnosis report, which includes information of pathogen types, bacterial/viral numbers, infection conditions and the like. Compared with the prior art, the invention has the advantages that: the artificial intelligent rapid pathogen detection method capable of rapidly judging pathogen information and accurately outputting diagnosis information based on artificial intelligence and application of the method in infectious disease diagnosis are provided.

Description

Artificial intelligent rapid pathogen detection method and application thereof in infectious disease diagnosis
Technical Field
The invention relates to the technical field of pathogen detection, in particular to an artificial intelligent rapid pathogen detection method and application thereof in infectious disease diagnosis.
Background
Pathogens refer to infectious microorganisms or biological agents, including bacteria, fungi, viruses, viroids, etc., that are capable of causing disease after being transmitted into a host. Food, water sources, soil, etc. can carry pathogens. If people are exposed to pathogen environment for a long time or eat food and water resources polluted by the pathogen, diseases such as gastroenteritis, hepatitis and the like can be caused in the human body.
At present, the detection means of pathogens are conventional detection methods, and after detecting pathogens, infection cannot be rapidly diagnosed, so that subsequent treatment is easily affected.
Disclosure of Invention
The invention aims to overcome the technical defects and provide an artificial intelligent rapid pathogen detection method capable of rapidly judging pathogen information and accurately outputting diagnosis information based on artificial intelligence and application of the method in infectious disease diagnosis.
In order to solve the technical problems, the technical scheme provided by the invention is as follows: an artificial intelligence rapid pathogen detection method comprising the steps of: step 1: collecting a sample; step 2: preparing a sample; step 3: detecting pathogens in the sample, and determining the types and the quantity of the pathogens; step 4: the detected data are analyzed and processed by using an artificial intelligence algorithm to generate a standardized diagnosis report, which includes information of pathogen types, bacterial/viral numbers, infection conditions and the like.
As a modification, in step 1, it is necessary to collect pathogens including blood, urine, respiratory secretions and the like from clinical samples.
In an improvement, in the step 2, the sample preparation includes pretreatment of the collected sample, including centrifugation, ultrasonic treatment, enzymatic digestion, deproteinization and the like of the sample, so as to improve the sensitivity and accuracy of the detection.
As an improvement, the step 3 comprises the steps of determining the pathogen content of the detection sample by means of high-throughput sequencing, mass spectrometry, PCR amplification, sequencing and the like.
In an improvement, in the step 4, result judgment is carried out according to an artificial intelligence algorithm and data information analysis.
Use of an artificial intelligence rapid pathogen detection method in the diagnosis of infectious diseases, comprising using the artificial intelligence rapid pathogen detection method of claims 1-5 for diagnosis of infectious diseases.
As an improvement, the etiology data is collected and processed by utilizing a database and cloud computing.
Compared with the prior art, the invention has the advantages that: the method comprises the steps of collecting etiology data information by using an artificial intelligence technology and adopting a database, comparing a pathogen detection result with the etiology database in real time, outputting accurate diagnosis information, greatly reducing the labor intensity of human beings and increasing the disease diagnosis efficiency.
Detailed Description
The present invention will be described in further detail below.
An artificial intelligence rapid pathogen detection method comprising the steps of: step 1: collecting a sample; step 2: preparing a sample; step 3: detecting pathogens in the sample, and determining the types and the quantity of the pathogens; step 4: the detected data are analyzed and processed by using an artificial intelligence algorithm to generate a standardized diagnosis report, which includes information of pathogen types, bacterial/viral numbers, infection conditions and the like.
In the step 1, pathogens including blood, urine, respiratory tract secretions and the like need to be collected from a clinical sample, in the step 2, sample preparation comprises pretreatment of the collected sample, including centrifugation, ultrasonic treatment, enzymatic digestion, deproteinization and the like of the sample, so as to improve the sensitivity and accuracy of detection, in the step 3, means such as high throughput sequencing, mass spectrometry, PCR amplification, sequencing and the like are included to determine whether the detection sample contains the pathogens, and in the step 4, the result is determined according to an artificial intelligence algorithm in combination with data information analysis.
By utilizing the technologies of a database, cloud computing and the like, a large amount of etiology data can be collected and processed, the speed and accuracy of pathogen detection are effectively improved, and reliable data support is provided for accurate medical treatment.
In the specific implementation of the invention, the information acquisition of the patient, including blood, urine, respiratory tract secretion and the like, the pretreatment of the acquired sample, including centrifugation, ultrasonic treatment, enzymatic digestion, deproteinization and the like of the sample are carried out, so that the sensitivity and accuracy of detection are improved, whether the detected sample contains pathogens or not is determined by means of high throughput sequencing, mass spectrometry, PCR amplification, sequencing and the like, and the output pathogen information is compared and analyzed by an artificial intelligence technology to determine the result.
What is not described in detail in this specification is prior art known to those skilled in the art.
The invention and its embodiments have been described above without limitation. In summary, if one of ordinary skill in the art is informed by this disclosure, a structural manner and an embodiment similar to the technical solution should not be creatively devised without departing from the gist of the present invention.

Claims (7)

1. The artificial intelligence rapid pathogen detection method is characterized in that: the method comprises the following steps:
step 1: collecting a sample;
step 2: preparing a sample;
step 3: detecting pathogens in the sample, and determining the types and the quantity of the pathogens;
step 4: the detected data are analyzed and processed by using an artificial intelligence algorithm to generate a standardized diagnosis report, which includes information of pathogen types, bacterial/viral numbers, infection conditions and the like.
2. The artificial intelligence rapid pathogen detection method of claim 1, wherein: in step 1, pathogens including blood, urine, respiratory secretions and the like need to be collected from clinical samples.
3. The artificial intelligence rapid pathogen detection method of claim 1, wherein: in the step 2, the sample preparation includes pretreatment of the collected sample, including centrifugation, ultrasonic treatment, enzymatic digestion, deproteinization, etc. of the sample, so as to improve the sensitivity and accuracy of the detection.
4. The artificial intelligence rapid pathogen detection method of claim 1, wherein: the step 3 comprises the steps of determining the pathogen content of the detection sample by means of high-throughput sequencing, mass spectrometry, PCR amplification, sequencing and the like.
5. The artificial intelligence rapid pathogen detection method of claim 1, wherein: and in the step 4, the result judgment is carried out according to the artificial intelligence algorithm and the data information analysis.
6. An application of an artificial intelligent rapid pathogen detection method in infectious disease diagnosis is characterized in that: comprising using the artificial intelligence rapid pathogen detection method of claims 1-5 for infectious disease diagnosis.
7. An application of an artificial intelligent rapid pathogen detection method in infectious disease diagnosis is characterized in that: the etiology data is collected and processed by utilizing a database and cloud computing.
CN202310853492.8A 2023-07-12 2023-07-12 Artificial intelligent rapid pathogen detection method and application thereof in infectious disease diagnosis Withdrawn CN116891890A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310853492.8A CN116891890A (en) 2023-07-12 2023-07-12 Artificial intelligent rapid pathogen detection method and application thereof in infectious disease diagnosis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310853492.8A CN116891890A (en) 2023-07-12 2023-07-12 Artificial intelligent rapid pathogen detection method and application thereof in infectious disease diagnosis

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CN116891890A true CN116891890A (en) 2023-10-17

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Application publication date: 20231017