CN116875438B - Microorganism sampling detection device in environment - Google Patents
Microorganism sampling detection device in environment Download PDFInfo
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- CN116875438B CN116875438B CN202311140289.2A CN202311140289A CN116875438B CN 116875438 B CN116875438 B CN 116875438B CN 202311140289 A CN202311140289 A CN 202311140289A CN 116875438 B CN116875438 B CN 116875438B
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- 238000005070 sampling Methods 0.000 title claims abstract description 112
- 244000005700 microbiome Species 0.000 title claims abstract description 40
- 238000001514 detection method Methods 0.000 title claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 238000003860 storage Methods 0.000 claims abstract description 28
- 238000001914 filtration Methods 0.000 claims description 21
- 239000000428 dust Substances 0.000 claims description 7
- 230000000813 microbial effect Effects 0.000 claims description 4
- 238000005192 partition Methods 0.000 claims description 4
- 230000002906 microbiologic effect Effects 0.000 claims 1
- 239000003570 air Substances 0.000 description 148
- 238000009434 installation Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012080 ambient air Substances 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
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- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application provides a microorganism sampling detection device in an environment, and belongs to the field of microorganism sampling equipment. The device for detecting the microorganism sampling in the environment comprises a buffer cylinder, a driving unit, at least two fan blades and a bracket. The driving shell of the driving unit drives the fan blades to rotate and simultaneously drives the intermediate gear to revolve around the main gear through the main gear, so that the buffer storage cylinder and the fan blades are driven to rotate in the same direction and form a rotating speed difference, the buffer storage cylinder rotates in a reciprocating mode, and simultaneously the two fan blades rotate in the two arc-shaped air chambers of the buffer storage cylinder in a reciprocating mode, and further the two arc-shaped air chambers suck external air through the unidirectional air inlets and blow the air into the liquid sampling assembly; the buffer cylinder rotates forwards and backwards along the circumferential direction, so that the unidirectional air inlet moves horizontally near the sampling point in a small amplitude, and air with the same height as the sampling point is collected as much as possible, the air in the up-down direction is reduced, and the sampling error is reduced; the forward space and the reverse space can relay air to the liquid sampling assembly, so that the liquid sampling assembly is always in a sampling state.
Description
Technical Field
The application belongs to the technical field of microorganism sampling equipment, and particularly relates to a microorganism sampling detection device in an environment.
Background
In production, many places have requirements on the concentration of microorganisms, such as sterile workshops, sterile laboratories, culture rooms, hatching rooms, etc., and these spaces need to be regularly tested for the concentration of microorganisms in the air to ensure that the cleanliness reaches the requirements. There are two general ways of detection that are now being performed: one is to collect the air at the appointed place and any height for detection, and the detection mode is suitable for the environment with no difference in the height of the microorganism concentration; the other is to collect air at a preset place and a designated height for detection, and the detection mode is suitable for environments with differences in microorganism concentration in height.
The sampling principle of the existing air microorganism sampler is as follows: air in the environment is sucked by the air pump and is led to pass through the sampling device (such as a sampling bottle, a sampling film and the like), so that microorganisms in the air are collected. Since the distribution of microorganisms in different environments has great difference, the concentration of microorganisms in a part of environments has difference in height direction, and at this time, sampling is required to be performed at the height where the concentration of microorganisms is maximum as much as possible. However, when the existing air microorganism sampler samples an environment with a difference in microorganism concentration in height, after the air microorganism sampler is placed at a specified height of a predetermined place, air around and in the up-down direction can be supplemented due to the fact that the sampler continuously sucks ambient air, wherein the microorganism concentration in the air in the up-down direction is different from the microorganism concentration at the specified height, sampling errors are increased, and accuracy of a detection result is reduced.
Disclosure of Invention
In view of the above, the embodiment of the application provides a device for sampling and detecting microorganisms in an environment, which solves the technical problem that in the prior art, air in the vertical direction is easy to collect, so that sampling errors are increased.
In order to achieve the above purpose, the application adopts the following technical scheme:
in one aspect, there is provided an apparatus for sampling and detecting microorganisms in an environment, comprising:
the buffer storage cylinder is cylindrical, the inside of the buffer storage cylinder is divided into at least two arc-shaped air chambers distributed along the circumferential direction, and the circumferential two ends of each arc-shaped air chamber are respectively provided with a one-way air inlet and a one-way air outlet;
the driving unit is provided with a driving shell and a fixed inner shaft which rotates reciprocally relative to the shell, and is arranged in the buffer memory cylinder, and the fixed inner shaft is coaxially arranged at the center shaft position of the buffer memory cylinder;
the fan blades are respectively positioned in each arc-shaped air chamber, each fan blade partitions the arc-shaped air chamber into a forward space and a reverse space in the circumferential direction, and the forward space and the reverse space respectively correspond to a one-way air inlet and a one-way air outlet at two ends of the arc-shaped air chamber; the two fan blades are fixed with the driving shell, and the driving shell drives the fan blades to rotate in a reciprocating manner so as to compress the forward space and expand the reverse space or compress the reverse space and expand the forward space;
the bracket is provided with a liquid sampling assembly fixed position, and an air inlet of the liquid sampling assembly is communicated with the one-way air outlet of at least one forward space and at least one reverse space;
the top of the support is provided with an annular gear, the buffer cylinder is arranged above the support, the lower end of the fixed inner shaft is fixed with the support, the lower end of the driving shell is coaxially fixed with a main gear, the main gear is coaxially positioned in the annular gear, the bottom of the buffer cylinder is also provided with an intermediate gear positioned in a gap between the main gear and the annular gear, and the intermediate gear is respectively meshed with the main gear and the annular gear;
the driving shell drives the fan blades to rotate, and meanwhile, the main gear drives the intermediate gear to revolve around the main gear, so that the buffer storage cylinder and the fan blades are driven to rotate in the same direction.
In some embodiments, each one-way air inlet is provided with an air inlet pipe extending upwards, the inlet of each air inlet pipe is higher than the top of the buffer cylinder, and the inlet of each air inlet pipe faces horizontally.
In some embodiments, each air inlet pipe is provided with a rotary joint, a rotating shaft of the rotary joint is parallel to the fixed inner shaft, an inlet end of the air inlet pipe horizontally extends, and an upper end of the driving shell extends out of the buffer cylinder and drives the inlet end of the air inlet pipe to horizontally rotate.
In some embodiments, a first pulley is arranged at the upper end of the driving shell, a second pulley is arranged at the inlet end of the air inlet pipe, and the first pulley is connected with the second pulley through a tensioning belt.
In certain embodiments, the inlet of the air inlet tube is at least 10cm above the top of the cache cylinder.
In some embodiments, the liquid sampling assembly is fixedly positioned as a perforated tray on the bracket adapted to receive the liquid sampling assembly.
In some embodiments, the perforated tray is rotatably coupled to the bracket such that the perforated tray can follow rotation under the pull of an external force.
In some embodiments, the air inlet pipe is detachably connected with the unidirectional air inlet, and a mounting groove is formed in the connecting end of the air inlet pipe, and the mounting groove is suitable for mounting a filtering type sampling unit or a dust filtering unit.
The microbial sampling detection device in the environment provided by the embodiment of the application has the beneficial effects that: compared with the prior art, the device for detecting the microorganism sampling in the environment has the advantages that the driving unit reciprocally rotates, on one hand, the two fan blades are driven to reciprocally rotate in the two arc-shaped air chambers of the buffer cylinder, so that the two arc-shaped air chambers suck external air through the unidirectional air inlet, and the air is blown into the liquid sampling assembly; on the other hand, the gear set drives the buffer cylinder to rotate in a circumferential direction in a reciprocating manner, so that the unidirectional air inlet moves horizontally near the sampling point in a small extent, and therefore air with the same height as the sampling point is collected as much as possible, the air in the vertical direction is reduced, and the sampling error is reduced;
in addition, the buffer cylinder is provided with two arc-shaped air chambers, and the air inlet of the liquid sampling assembly is communicated with the unidirectional air outlets of the at least one forward space and the at least one reverse space, so that in the reciprocating rotation process of the fan blade in the two arc-shaped air chambers of the buffer cylinder, the at least one forward space and the at least one reverse space can blow air into the liquid sampling assembly in a relay manner, and the liquid sampling assembly is always in a sampling state;
in addition, the drive unit is arranged inside the buffer storage cylinder, and the buffer storage cylinder and the blades inside the buffer storage cylinder are driven to rotate in the same direction through the gear set at the top of the support to form a rotating speed difference, so that the buffer storage cylinder rotates and the blades inside the buffer storage cylinder rotate relative to the buffer storage cylinder to realize sampling, and the buffer storage cylinder and the blades rotate in the same direction, so that the maximum rotating angle of the blades in the arc-shaped air chamber cannot limit the maximum rotating angle of the buffer storage cylinder, the buffer storage cylinder can rotate at a larger angle, and air in a larger horizontal range is collected.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments or the description of the prior art will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a front view of a device for detecting microbial samples in an environment provided by an embodiment of the present application;
FIG. 2 is a top view of the internal structure of the cache cylinder of FIG. 1, wherein the fan blades are in an initial position;
FIG. 3 is a top view of the inner structure of the cache cylinder when the fan blade of FIG. 2 is rotated to the reverse position;
fig. 4 is a schematic view of the internal structure of the intake pipe.
Wherein, each reference sign in the figure:
1-a cache cylinder; 11-arc-shaped air chambers; 12-a one-way air inlet; 13-a one-way air outlet; 14-an intermediate gear; 2-fixing the inner shaft; 21-a drive housing; 22-a main gear; 23-a first pulley; 3-fan blades; 31-forward space; 32-a reverse space; 4-a bracket; 41-a perforated pallet; 42-a liquid sampling assembly; 43-ring gear; 5-an air inlet pipe; 51-revolute joint; 52-a second pulley; 53-mounting groove; 54-filtering sampling unit.
Detailed Description
In order to make the technical problems, technical schemes and beneficial effects to be solved more clear, the application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the application.
It will be understood that when an element is referred to as being "mounted" or "disposed" on another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
It is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are merely for convenience in describing and simplifying the description based on the orientation or positional relationship shown in the drawings, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus are not to be construed as limiting the application.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" or "a number" means two or more, unless specifically defined otherwise.
Referring to fig. 1 to 4, a device for detecting microorganism sampling in an environment according to an embodiment of the application will be described. A device for sampling and detecting microorganisms in an environment comprising: the device comprises a cache cylinder 1, a driving unit, at least two fan blades 3 and a bracket 4.
The buffer cylinder 1 is cylindrical, the inner part is at least two arc-shaped air chambers 11 distributed along the circumferential direction, and the circumferential two ends of each arc-shaped air chamber 11 are respectively provided with a one-way air inlet 12 and a one-way air outlet 13. Specifically, the number of the arc-shaped air cells 11 may be two or three.
The driving unit is provided with a driving shell 21 and a fixed inner shaft 2 which rotates reciprocally relative to the shell, and is arranged in the buffer memory cylinder 1, and the fixed inner shaft 2 is coaxially arranged at the center axis position of the buffer memory cylinder 1.
At least two fan blades 3 are respectively positioned in each arc-shaped air chamber 11, each fan blade 3 partitions the arc-shaped air chamber 11 into a forward space 31 and a reverse space 32 in the circumferential direction, and the forward space 31 and the reverse space 32 respectively correspond to the unidirectional air inlets 12 and the unidirectional air outlets 13 at two ends of the arc-shaped air chamber 11; the two blades 3 are fixed to the driving housing 21, and the driving housing 21 drives the blades 3 to reciprocate to compress the forward space 31 and expand the reverse space 32, or compress the reverse space 32 and expand the forward space 31. Specifically, the number of the fan blades 3 may be two or three.
The holder 4 has a liquid sampling assembly 42 fixed in position, the inlet of the liquid sampling assembly 42 being in communication with the unidirectional outlet 13 of the at least one forward space 31 and the at least one reverse space 32.
The top of the bracket 4 is provided with an annular gear 43, the buffer cylinder 1 is arranged above the bracket 4, the lower end of the front fixed inner shaft 2 is fixed with the bracket 4, the lower end of the driving shell 21 is coaxially fixed with a main gear 22, the main gear 22 is coaxially arranged in the annular gear 43, the bottom of the buffer cylinder 1 is also provided with an intermediate gear 14 arranged in a gap between the main gear 22 and the annular gear 43, and the intermediate gear 14 is respectively meshed with the main gear 22 and the annular gear 43.
The driving shell 21 drives the fan blades 3 to rotate, and simultaneously drives the intermediate gear 14 to revolve around the main gear 22 through the main gear 22, so that the buffer cylinder 1 and the fan blades 3 are driven to rotate in the same direction.
Compared with the prior art, the device for detecting the microorganism sampling in the environment has the advantages that the driving unit reciprocally rotates, on one hand, the two fan blades 3 are driven to reciprocally rotate in the two arc-shaped air chambers 11 of the buffer cylinder 1, so that the two arc-shaped air chambers 11 suck external air through the unidirectional air inlet 12 and blow the air into the liquid sampling assembly 42; on the other hand, the gear set drives the buffer cylinder 1 to rotate in a circumferential direction in a reciprocating manner, so that the unidirectional air inlet 12 moves horizontally in a small amplitude near the sampling point, air with the same height as the sampling point is collected as much as possible, air in the vertical direction is reduced, and sampling errors are reduced.
Moreover, the buffer cylinder 1 is provided with two arc-shaped air chambers 11, and the air inlet of the liquid sampling assembly 42 is communicated with the unidirectional air outlets 13 of the at least one forward space 31 and the at least one reverse space 32, so that in the reciprocating rotation process of the fan blade 3 in the two arc-shaped air chambers 11 of the buffer cylinder 1, the at least one forward space 31 and the at least one reverse space 32 can blow air into the liquid sampling assembly 42 in a relay manner, and the liquid sampling assembly 42 is always in a sampling state.
In addition, the drive unit is arranged inside the buffer storage cylinder 1, and the buffer storage cylinder 1 and the internal fan blades 3 are driven to rotate in the same direction through the gear set at the top of the support 4 to form a rotating speed difference, so that the buffer storage cylinder 1 rotates and the internal fan blades 3 rotate relative to the buffer storage cylinder 1 to realize sampling, and the buffer storage cylinder 1 and the fan blades 3 rotate in the same direction, so that the maximum rotation angle of the fan blades 3 in the arc-shaped air chamber 11 can not limit the maximum rotation angle of the buffer storage cylinder 1, the buffer storage cylinder 1 can rotate at a larger angle, and air in a larger horizontal range is collected.
In this embodiment, the buffer cartridge 1 may have a cylindrical housing, and the interior of the housing is uniformly divided into a plurality of arc-shaped air cells 11 by a partition plate along a radial direction, and the number of the arc-shaped air cells 11 is preferably two or three. Both ends of each arc-shaped air chamber 11 are provided with an inward check valve and an outward check valve, thereby forming a one-way air inlet 12 and a one-way air outlet 13. Preferably, the unidirectional air intake 12 is oriented horizontally to facilitate absorption of air in the horizontal direction; the unidirectional air outlet 13 is mainly used for conveniently connecting a liquid sampling assembly 42 (sampling bottle, sampling box and the like), and can be positioned at the bottom of the buffer bucket. The volume of the arc-shaped air chamber 11 inside the buffer cartridge 1 can be increased by increasing the height or diameter of the buffer cartridge 1, and is selected according to the specific situation. For example, when there is a strict limitation on the sampling range of the sampling point, a buffer bucket with a smaller diameter and a larger height may be selected; and when the requirement on the sampling range of the sampling point is loose, a caching barrel with a larger diameter can be selected.
The outside of the drive unit may be a cylindrical drive housing 21, the central axis of the drive housing 21 being the stationary inner shaft 2. The driving unit is arranged at the middle shaft position of the buffer storage cylinder 1, and the upper end and the lower end are respectively sealed with the buffer storage cylinder 1. The drive unit may in particular consist of a stepper motor and a reducer, the output shaft of which forms the fixed inner shaft 2.
Each fan blade 3 is arranged in the corresponding arc-shaped air chamber 11, the shape of each fan blade 3 is the same as the cross-section shape of the arc-shaped air chamber 11, and rubber sealing strips are arranged at the edges of the fan blades 3, so that the tightness with the inner wall of the arc-shaped air chamber 11 is improved. Each fan blade 3 is directly fixed to the outside of the drive housing 21. When the driving unit is started, the fixed inner shaft 2 is fixed with the bracket 4, and the driving shell 21 directly drives the fan blades 3 to rotate.
The bracket 4 is mainly used for fixing the liquid sampling assembly 42 and supporting the buffer cartridge 1 above. The support 4 can also adopt a lifting and folding structure in the prior art so as to facilitate height adjustment and storage. The top of the bracket 4 is a horizontal flat plate, and the annular gear 43 is horizontally fixed on the flat plate. The lower end of the driving unit passes through the buffer tube 1, and the main gear 22 is coaxially fixed at the lower end of the driving housing 21, the main gear 22 is coaxially positioned in the annular gear 43, and a circle of gap is formed between the annular gear 43 and the main gear 22. The bottom of the buffer container 1 is rotatably provided with a plurality of intermediate gears 14, and the intermediate gears 14 are positioned in the gaps between the main gears 22 and the annular gears 43 and are respectively meshed with the main gears 22 and the annular gears 43, so that when the main gears 22 rotate, the intermediate gears 14 can be driven to rotate and revolve around the main gears 22 at the same time, thereby driving the buffer container 1 to rotate. The fixed inner shaft 2 in the center of the buffer memory cylinder 1 passes through the main gear 22 and is directly fixed with the bracket 4 at the lower end, so that the stability of the whole structure is ensured.
In particular, with reference to fig. 2 and 3, the blades 3 are rotated from the initial position to the inverted position, the blades 3 are rotated by an angle of about 270 °, and the cache cylinder 1 is rotated by an angle of about 120 °. The reciprocating rotation speed of the buffer cylinder 1 is determined according to the natural circulation speed of air, the diffusion speed of microorganisms and other factors in the sampling environment, so that the microorganism concentration in the original position can be naturally recovered to a certain degree before the buffer cylinder 1 is reset as much as possible. For example, in some cases, the microorganism concentration at the sampling point is expected to recover to a level of about 80% in 1 minute to 10 minutes, and the period of reciprocation is only required to be 2 minutes to 15 minutes. Of course, the use of the present embodiment is not limited to this, and the reciprocating rotation speed of the buffer cartridge 1 may be increased or decreased according to the actual situation.
Referring to fig. 1, as a specific embodiment of the device for detecting microorganism sampling in an environment provided by the present application, each unidirectional air inlet 12 is provided with an air inlet pipe 5 extending upwards, the inlet of each air inlet pipe 5 is higher than the top of the cache cylinder 1, and the inlet of the air inlet pipe 5 faces horizontally.
In this embodiment, the inlet of the air inlet pipe 5 is higher than the top of the cache cylinder 1, so that the cache cylinder 1 can be prevented from blocking the air in the horizontal direction from moving to the inlet of the air inlet pipe 5, and the air inlet pipe 5 can collect the air in the horizontal direction near the sampling point more smoothly. In concrete implementation, each bending part of the air inlet pipe 5 adopts a large-radian round corner, so that air inlet resistance is reduced.
Referring to fig. 1, as a specific embodiment of the device for detecting microorganism sampling in the environment provided by the present application, each air inlet pipe 5 is provided with a rotary joint 51, the rotation axis of the rotary joint 51 is parallel to the fixed inner shaft 2, the inlet end of the air inlet pipe 5 extends horizontally, and the upper end of the driving housing 21 extends out of the buffer tube 1 and drives the inlet end of the air inlet pipe 5 to rotate horizontally.
In this embodiment, by setting the rotation joint 51, the air inlet pipe 5 can rotate horizontally along with the rotation of the buffer drum 1, collect the air in the horizontal direction around, and further increase the collected horizontal range.
In a specific implementation, the middle portion of the air intake pipe 5 may employ a rotary joint as the rotary joint 51, the direction of which is vertically parallel to the upper end of the driving housing 21. The length of the inlet end of the air inlet pipe 5 extending horizontally may be determined as appropriate, for example, when the diameter of the cache cylinder 1 is large, the inlet end horizontal length of the air inlet pipe 5 may be increased.
The upper end of the driving housing 21 may be a gear, a chain, a tension belt, etc. to drive the inlet end of the air inlet pipe 5 to horizontally rotate. The rotation speed of the inlet end of the air inlet pipe 5 is regulated by the gear, the chain wheel, the gear ratio of the belt wheel or the diameter ratio.
Referring to fig. 1, as a specific embodiment of the device for detecting microorganism sampling in an environment provided by the present application, a first belt pulley 23 is disposed at an upper end of a driving housing 21, a second belt pulley 52 is disposed at an inlet end of an air inlet pipe 5, and the first belt pulley 23 is connected to the second belt pulley 52 through a tensioning belt.
In a specific implementation, the first pulley 23 may be a second pulley 52 having a plurality of grooves, and a plurality of tension bands may be installed to connect the inlet ends of different air inlet pipes 5.
Referring to fig. 1, as an embodiment of the device for detecting microorganism sampling in the environment provided by the present application, the inlet of the air inlet pipe 5 is at least 10cm higher than the top of the buffer tank 1.
According to the embodiment, the blocking of the buffer cylinder 1 to the air in the horizontal direction can be reduced to the greatest extent, and the air in the horizontal direction can be absorbed by the inlet of the air inlet pipe 5.
Referring to fig. 4, as a specific embodiment of the device for detecting microorganism sampling in the environment provided by the present application, an air inlet pipe 5 is detachably connected with a unidirectional air inlet 12, a mounting groove 53 is provided in a connection end of the air inlet pipe, and a filtering type sampling unit 54 or a dust filtering unit is adapted to be installed in the mounting groove 53.
In actual sampling, it is often the case that different sampling points need to be sampled by using different sampling media, and even the same sampling point often needs to collect different samples by using different sampling media.
In this embodiment, during normal sampling, the unidirectional air outlet 13 is connected with the liquid sampling assembly 42, and samples are taken through the liquid sampling assembly 42, and at this time, a dust filtering unit is installed in the installation groove 53 of the air inlet pipe 5, so as to filter out large particles in the air. For the place requiring the filtering type sampling by using the diaphragm, the filtering type sampling unit 54 can be directly installed in the installation groove 53 of the air inlet pipe 5 (refer to the prior art, and substances in the air are filtered by the diaphragm), so that not only can the effect of accurately collecting the air at the same height be realized, but also the air can directly enter the filtering type sampling unit 54 through the air inlet pipe 5, compared with the place connected with the unidirectional air outlet 13, the flow path of the air can be greatly reduced, and the sampling precision is improved.
In addition, since the buffer cartridge has two arc-shaped air cells 11, and each arc-shaped air cell 11 breathes air independently at the time of sampling. Therefore, the liquid sampling component 42 can be connected with the unidirectional air outlet 13 of one arc-shaped air chamber 11, and the filtering type sampling unit 54 is arranged in the air inlet pipe 5 of the unidirectional air inlet 13 of the other arc-shaped air chamber 11, so that two samples can be collected at one time at the same place.
In particular, the air inlet pipe 5 is installed on the unidirectional air inlet 12 of the buffer cylinder 1 through threads. A cylindrical cavity is provided inside one end of the air inlet pipe 5 for connection as a mounting groove 53 (the shape of the cavity is determined by the shape of the filtering type sampling unit, and the shape of the collecting unit used by the inventor is cylindrical, so that the cylindrical shape is adopted), when the air inlet pipe 5 is mounted on the unidirectional air inlet 12, the filtering type sampling unit 54 is clamped and fixed at the mounting groove 53, and after sampling is finished, the air inlet pipe 5 can be detached, and the filtering type sampling unit 54 is taken out. The shape of the dust filtering unit is determined according to the shape of the installation groove 53, and the mesh density thereof is selected according to the actual filtering requirements. Specifically, the pipe can be cylindrical as a whole, the outside is a circular pipe, and the inside of the circular pipe is plugged by adopting a plurality of layers of stainless steel nets.
In practical implementation, the depth of the installation groove 53 may be set so that the filtering type sampling unit 54 and the dust filtering unit may be installed at the same time, and air may be filtered by the dust filtering unit and then enter the filtering type sampling unit 54.
Referring to fig. 1, as an embodiment of the device for detecting the sampling of microorganisms in the environment provided by the present application, the liquid sampling assembly 42 is fixedly positioned on the support 4 and is provided with a perforated tray 41 adapted to be inserted with the liquid sampling assembly 42.
Referring to fig. 1, as an embodiment of the device for detecting microorganism sampling in the environment provided by the present application, a perforated tray 41 is rotatably connected to a bracket 4, so that the perforated tray 41 can rotate under the traction of an external force.
In this embodiment, the perforated support plate 41 is configured to rotate, when the buffer tube 1 rotates at a larger angle, the liquid sampling assembly 42 on the perforated support plate 41 can rotate at a certain angle along with the buffer tube 1, so as to avoid that the buffer tube 1 will tear off the connecting tube with the liquid sampling assembly 42 or pull the liquid sampling assembly 42 off from the perforated support plate 41.
In a specific implementation, the perforated support plate 41 is a long strip plate, and the middle part of the long strip plate is rotatably connected with the bracket 4, so that the perforated support plate 41 can freely rotate horizontally. The two ends of the perforated supporting plate 41 are respectively provided with a placing hole, a liquid sampling assembly 42 can be placed, and the liquid sampling assembly 42 is connected with the unidirectional air outlet 13 through a hose.
The foregoing description of the preferred embodiments of the application is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the application.
Claims (8)
1. Microorganism sampling detection device in environment, its characterized in that includes:
the buffer storage cylinder is cylindrical, the inside of the buffer storage cylinder is divided into at least two arc-shaped air chambers distributed along the circumferential direction, and the circumferential two ends of each arc-shaped air chamber are respectively provided with a one-way air inlet and a one-way air outlet;
the driving unit is provided with a driving shell and a fixed inner shaft which rotates reciprocally relative to the shell, and is arranged in the buffer memory cylinder, and the fixed inner shaft is coaxially arranged at the center shaft position of the buffer memory cylinder;
the fan blades are respectively positioned in each arc-shaped air chamber, each fan blade partitions the arc-shaped air chamber into a forward space and a reverse space in the circumferential direction, and the forward space and the reverse space respectively correspond to a one-way air inlet and a one-way air outlet at two ends of the arc-shaped air chamber; the two fan blades are fixed with the driving shell, and the driving shell drives the fan blades to rotate in a reciprocating manner so as to compress the forward space and expand the reverse space or compress the reverse space and expand the forward space;
the bracket is provided with a liquid sampling assembly fixed position, and an air inlet of the liquid sampling assembly is communicated with the one-way air outlet of at least one forward space and at least one reverse space;
the top of the support is provided with an annular gear, the buffer cylinder is arranged above the support, the lower end of the fixed inner shaft is fixed with the support, the lower end of the driving shell is coaxially fixed with a main gear, the main gear is coaxially positioned in the annular gear, the bottom of the buffer cylinder is also provided with an intermediate gear positioned in a gap between the main gear and the annular gear, and the intermediate gear is respectively meshed with the main gear and the annular gear;
the driving shell drives the fan blades to rotate, and meanwhile, the main gear drives the intermediate gear to revolve around the main gear, so that the buffer storage cylinder and the fan blades are driven to rotate in the same direction.
2. The apparatus according to claim 1, wherein each of the unidirectional air inlets is provided with an air inlet pipe extending upward, an inlet of each of the air inlet pipes is higher than a top of the buffer cartridge, and an inlet of the air inlet pipe is oriented horizontally.
3. The device for detecting the microbial sampling in the environment according to claim 2, wherein each air inlet pipe is provided with a rotary joint, a rotating shaft of the rotary joint is parallel to the fixed inner shaft, an inlet end of the air inlet pipe horizontally extends, and an upper end of the driving shell extends out of the buffer cylinder and drives the inlet end of the air inlet pipe to horizontally rotate.
4. A device for detecting the sampling of microorganisms in the environment according to claim 3, wherein the upper end of the driving housing is provided with a first belt wheel, the inlet end of the air inlet pipe is provided with a second belt wheel, and the first belt wheel is connected with the second belt wheel through a tension belt.
5. The device for detecting the sampling of microorganisms in an environment according to claim 2, wherein the inlet of the air inlet pipe is at least 10cm higher than the top of the buffer cartridge.
6. The in-environment microbiological sampling test device of claim 1 wherein the liquid sampling assembly is fixedly positioned as a perforated tray on the support adapted to receive the liquid sampling assembly.
7. The device of claim 6, wherein the perforated tray is rotatably coupled to the frame such that the perforated tray can follow rotation under the pull of an external force.
8. The device for detecting the microbial samples in the environment according to any one of claims 2 to 5, wherein the air inlet pipe is detachably connected with the unidirectional air inlet, a mounting groove is formed in the connecting end of the air inlet pipe, and a filtering type sampling unit or a dust filtering unit is arranged in the mounting groove.
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