CN115261207A

CN115261207A - Sampling and detection integrated device for air microorganisms

Info

Publication number: CN115261207A
Application number: CN202211061710.6A
Authority: CN
Inventors: 辛燕花; 罗伟强
Original assignee: Xinzhou Teachers University
Current assignee: Xinzhou Teachers University
Priority date: 2022-08-31
Filing date: 2022-08-31
Publication date: 2022-11-01

Abstract

The invention relates to the technical field of microorganism sampling detection, in particular to an air microorganism sampling and detecting integrated device. The sampling device comprises a sampling body, wherein the sampling body comprises an air suction component, an intermediate component and a sample preparation component, the air suction component is formed in an air flow channel, the air suction component is arranged at the top of the intermediate component, and the sample preparation component is arranged at the bottom of the intermediate component. According to the invention, air is sucked into the air flow channel through the air suction component to form air flow, active sampling is carried out through the air suction component, the round swab is used for intercepting microorganisms in the air flow, active sampling is not required through the round swab, and the air sucked by the air suction component is mainly matched to carry out sample preparation, so that a sample is provided for detection of a detection body, the integration of sampling and detection is realized, the problem of low detection efficiency after plate sampling is solved, and the problem that the swab cannot directly sample the microorganisms in the air is also solved.

Description

Sampling and detection integrated device for air microorganisms

Technical Field

The invention relates to the technical field of microorganism sampling detection, in particular to an air microorganism sampling and detecting integrated device.

Background

The microorganisms in the air mainly come from human life and production processes, the microorganisms are ubiquitous in the air, they are always around us, they are attached to dust or liquid drops, and they are suspended in the air along with carriers.

ATP is an energy unit contained in living cells of all animals and plants, bacteria, molds, yeasts, and the like, and all microorganisms contain ATP, and therefore, detection of ATP can be used as an important index for determining cleanliness, for example:

chinese patent publication No. CN111763614A discloses a biological aerosol on-line monitoring system and method based on ATP biochemical luminescence, active microbial aerosols in the ambient air are collected by a large-flow biological aerosol sampling technology, ATP is released by cracking microbial cells contained in the aerosols by combining an ATP biochemical luminescence reaction principle, the ATP and luciferin generate chemical reaction luminescence under catalysis of luciferase, luminescence signals are converted into electric signals by a photoelectric sensor to be recorded and output, and rapid enrichment and real-time detection of low-concentration active microorganisms in the air are realized.

Chinese patent publication No. CN214794405U discloses a device for detecting ATP fluorescence microorganism, which sucks a water sample into a tube body through a suction pipe and a water absorption part arranged in the tube body by atmospheric pressure intensity, the outer wall of the tube body is provided with scales, the water absorption part can discharge at any time and suck air to carry out precise quantitative suction on the water sample, the detection accuracy is ensured, in addition, through the plug-in fit of a reagent tube and a release tube, a lysate and a reaction liquid in the reagent tube can be released to enter the tube body without breaking a swab off the tube, the tube body can not be greatly swung, and the possibility of device damage caused by reduction is reduced.

It can be seen that the prior art has proposed the inconvenience of sampling swabs, but i have perceived that swabs not only have the above problems, but also: because of the particularity of the air, it is difficult to directly sample the air by a swab, and it is difficult to rapidly obtain the result if a plate is used for sample preparation.

Disclosure of Invention

The invention aims to provide an integrated device for sampling and detecting air microorganisms, which aims to solve the problems in the background technology.

In order to achieve the above object, there is provided an integrated sampling and detecting device for airborne microorganisms, comprising a sampling body, wherein the sampling body comprises an air intake component, an intermediate component and a sample preparation component, the air intake component is arranged at the top of the intermediate component, the sample preparation component is arranged at the bottom of the intermediate component, air inhaled by the air intake component enters the sample preparation component through an air flow passage for sample preparation, and the integrated sampling and detecting device further comprises a detecting body arranged at the bottom of the sampling body, the intermediate component is of a telescopic structure, and the flow radius of the air flow passage is changed by the expansion and contraction of the intermediate component, wherein:

during sampling, the intermediate member contracts, and the radius of the air flow channel is increased;

after sample preparation, the middle component extends outwards, the radius of the air flow channel is reduced, the pressure of air forming airflow is increased, and a sample obtained by pressing the sample preparation enters a detection body for detection.

As a further improvement of the technical solution, the detecting body includes a detecting end and a display end, and the display end is arranged at one side of the detecting end and is used for displaying digital information detected by the detecting end;

the top of the detection end is provided with a detection head, the top of the detection head is provided with a sampling groove, and the detection head connected with the sample preparation component extends into an air flow channel in the sample preparation component.

As a further improvement of the technical scheme, the detection head is integrally connected with the sample preparation component.

As a further improvement of the technical scheme, the air suction component comprises a top cover and a base arranged at the bottom of the top cover, and the top cover and the base are connected to form an air inlet chamber;

the air inlet chamber is rotationally connected with fan blades;

a central seat fixed on the base is arranged in the air inlet cavity, and a motor for driving the fan blades to rotate is arranged on the central seat.

As a further improvement of the technical scheme, the intermediate member comprises an outer tube and an inner tube, an intermediate chamber is formed between the outer tube and the inner tube, a sliding opening in sliding connection with the inner tube is formed in the bottom of the outer tube, and a telescopic structure is formed by the sliding connection of the outer tube and the inner tube;

a flexible seat is arranged at the top of the inner pipe, and a blocking surface is arranged above the flexible seat in the middle cavity;

the flexible seat is an annular structure having a channel therein, wherein: in a normal state of the flexible seat, the radius of the channel is smaller than that of the air flow channel; when the inner pipe contracts towards the inner part of the outer pipe, the blocking surface extrudes the flexible seat to expand the internal channel of the flexible seat.

As a further improvement of the technical scheme, the outer tube and the base are integrally connected.

As a further improvement of the technical scheme, the sample preparation component comprises a sample preparation seat and a sample preparation carrier plate, wherein an air inlet channel penetrates through the sample preparation seat;

a sample preparation cavity for accommodating a sample preparation carrier plate is formed in the side surface of the sample preparation seat, and a loading groove for bearing a sample preparation surface is formed in the top of the sample preparation carrier plate;

the bottom of the carrying groove is provided with a notch.

As a further improvement of the technical scheme, the detection end and the sample preparation component are detachably connected.

As a further improvement of the technical scheme, the outer pipe and the base are of a split structure, an outer ring plate is arranged on the periphery of the joint of the base and the outer pipe, the base and the outer pipe are fixed through the outer ring plate, and the periphery of the outer ring plate protrudes outwards.

As a further improvement of the technical scheme, the flexible seat consists of a stress part and a supporting part, the stress part is arranged at the top of the supporting part, and a V-shaped structure is formed between the stress part and the supporting part.

Compared with the prior art, the invention has the following beneficial effects:

1. this air microorganism's sampling detects integrated device, inhale the air through the air suction component in to the air runner, form the air current, carry out initiative sampling through the air suction component like this, and circular swab is the microorganism that is used for intercepting in the air current, just so need not to carry out initiative sampling through circular swab, the inspiratory air of main cooperation air suction component carries out the preparation of sample can, thereby provide the sample for the detection of detecting the body, realize the integration of sampling and detection, solve the slow problem of detection efficiency after the flat dish sampling promptly, solve the problem that the swab can not directly carry out the sampling to the microorganism in the air again.

2. In the sampling and detecting integrated device for the air microorganisms, the state of air flow is changed through the expansion and contraction of the intermediate component, the sampling area needs to be enlarged when a sample is prepared, so that the radius of the air flow channel is enlarged through the extension of the intermediate component, the radius of the air flow channel is reduced through the contraction of the intermediate component during detection, the air flow pressure is increased, a round swab enters a detection body to be detected, the air flow is controlled and utilized in the whole process, and the condition that the swab is manually contacted is avoided.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is an exploded view of the overall structure of the present invention;

FIG. 3 is a schematic view of a detector structure according to the present invention;

FIG. 4 is a schematic view of a sample volume configuration of the present invention;

FIG. 5 is a schematic view of the air intake member of the present invention;

FIG. 6 is a side view of the air intake member of the present invention;

FIG. 7 is a schematic view of a plate construction of the present invention;

FIG. 8 is a schematic view of an intermediate member of the present invention;

FIG. 9 is a schematic view of the connection structure of the base and the outer tube of the present invention;

FIG. 10 is a schematic view of a sample preparation member according to the present invention;

FIG. 11 is a schematic side view of the flexible base of the present invention after being compressed;

fig. 12 is a schematic side view of the flexible seat of the present invention after repositioning.

The various reference numbers in the figures mean:

100. sampling a sample;

110. an air intake member; 111. a top cover; 1111. a fan blade; 112. a center seat; 112a, adduction surfaces; 112b, an external expanding surface; 110A, draining a cavity; 113. a base; 1131. a drainage seat; 114. a support plate; 114A, a through groove;

120. an intermediate member; 121. an outer tube; 1211. an outer ring plate; 121A, a sliding port; 122. an inner tube; 123. a flexible seat; 123a, a force-receiving portion; 123b, a support portion;

130. preparing a sample component; 131. preparing a sample seat; 131A, an air inlet channel; 131B, a sample preparation chamber; 132. preparing a sample support plate; 1321. an outer frame; 1322. a handle; 132A, a carrying groove; 132B, notches;

200. detecting a body;

210. a detection end; 211. a detection head; 211A, a sampling groove; 212. a suction cup;

220. a handle; 230. and a display end.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.

Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

The microorganisms in the air mainly come from human life and production processes, the microorganisms are everywhere in the air, the microorganisms are around us all the time, and the microorganisms are attached to dust or liquid drops and suspended in the air along with carriers.

ATP is an energy unit contained in living cells of all animals, plants, bacteria, molds, yeasts and the like, and all microorganisms contain ATP, so that ATP detection can be used as an important index for judging the cleanliness, but because of the particularity of air, the air is difficult to directly sample by swabs, and the result is difficult to rapidly obtain if a plate is adopted for sample preparation.

Therefore, the invention provides an integrated sampling and detecting device for air microorganisms, which comprises a sampling body 100 and a detecting body 200 arranged at the bottom of the sampling body 100, as shown in fig. 1, the sampling body 100 comprises an air suction component 110, an intermediate component 120 and a sample preparation component 130, wherein the air suction component 110 is arranged at the top of the intermediate component 120, the sample preparation component 130 is arranged at the bottom of the intermediate component 120, an air flow channel is directly formed by the air suction component 110, the air sucked by the air suction component 110 enters the sample preparation component 130 for sample preparation, the intermediate component 120 is of a telescopic structure, the flow radius of the air flow channel is changed by the expansion and contraction of the intermediate component 120, the radius of the air flow channel is increased during sampling, so as to ensure that the air enters the range of the sample preparation component 130, after sample preparation, the intermediate component 120 extends outwards, the radius of the air flow channel is decreased, the air flow pressure formed by the air is increased, and the sample prepared by pressing the sample prepared sample into the detecting body 200 for detection.

On the basis of the above-described structure, fig. 2, 3, 4, 5, 8 and 10 show a first embodiment of the present invention,

in fig. 2, the detecting body 200 includes a detecting end 210 and a displaying end 230, the displaying end 230 is disposed at one side of the detecting end 210 and is used for displaying digital information detected by the detecting end 210, the detecting end 210 detects the content of the microorganism in the sample by using ATP, here, adenosine Triphosphate (ATP) is rapidly detected by using a luciferase-luciferin system based on the firefly luminescence principle, since the number of the microorganism in the sample is in direct proportion to the ATP content in the microorganism, the content of the microorganism in the sample can be detected by the detecting end 210, and the displaying end 230 is used for digitally displaying the content.

It should be noted here that the fixed side of the detection end 210 may be placed on a table top or other plane capable of supporting the detection end 210, or a handle 220 may be disposed on the fixed side of the detection end 210 to support the detection end 210 in a handheld manner, so that the detection end 210 can be sampled and detected at a specified position, but the fixing of the detection end 210 is not limited to the above two manners, as long as the detection end 210 can be fixed at a specified position for sampling and detection, and such structures are many, for example, the detection end 210 is fixed on a wall by bolts, and therefore, redundant description is not repeated here.

As shown in fig. 3, a detection head 211 is disposed on the top of the detection end 210, a sampling groove 211A is disposed on the top of the detection head 211, the detection head 211 is integrally connected to the sample preparation member 130, and the connected detection head 211 extends into an air flow channel of the sample preparation member 130 to receive an incoming sample by using the sampling groove 211A, and the received sample is matched with the detection end 210 to complete detection of the content of microorganisms in the sample.

As to the structure of the air inhaling component 110 inhaling the air in the environment, as shown in fig. 5, the air inhaling component 110 includes a top cover 111 and a base 113 disposed at the bottom of the top cover 111, the top cover 111 and the base 113 are connected to form an air intake chamber, the air intake chamber also forms a part of the air flow channel, first, the top cover 111 and the base 113 are a hollow structure with a top and a bottom penetrating through, which also forms the basis of the air intake chamber, then the fan 1111 is rotatably connected in the air intake chamber, a center seat 112 is disposed in the air intake chamber, a motor is disposed on the center seat 112, an output shaft of the motor is fixedly connected to a rotating shaft of the fan 1111, the motor can drive the fan 1111 to rotate after being powered on, the rotating fan 1111 sucks the air in the environment into the air intake chamber, which is the first step of the air entering the air flow channel, and forms an air flow in the air flow channel, and the direction of the air flow is towards the detecting body 200.

The center base 112 is fixed on the base 113, in this embodiment, a plurality of fixing plates are disposed on the periphery of the center base 112, and the center base 112 is fixed by connecting one end of each fixing plate to the inner wall of the base 113 and connecting the other end of each fixing plate to the center base 112, and the fan blades 1111 are rotatably connected to the top cover 111.

Next, fig. 8 shows a specific structure of the intermediate member 120, the intermediate member 120 includes an outer tube 121 and an inner tube 122, the outer tube 121 and the inner tube 122 are both hollow structures with top portions and top portions penetrating through, the hollow portions form a telescopic intermediate chamber, the intermediate chamber also forms a part of the air flow channel, and after the outer tube 121 is hollow, a sliding opening 121A slidably connected with the inner tube 122 is formed at the bottom of the outer tube 121, that is, the outer tube 121 and the inner tube 122 are slidably connected to form a telescopic structure, and the telescopic function of the intermediate chamber is also achieved by the inner tube 122 sliding in the sliding opening 121A;

furthermore, the top of the inner tube 122 is provided with a flexible seat 123, and at this time, a blocking surface is required to be arranged above the flexible seat 123 in the middle chamber, and the blocking surface may be an inner edge (not shown) arranged inside the outer tube 121, or an inner edge (not shown) arranged inside the base 113 to form a blocking surface, where the flexible seat 123 is an annular structure, and a channel through which the air flow can pass is arranged inside the flexible seat 123, and in a normal state, the radius of the channel is smaller than the radius of the air flow channel, so that the pressure can be increased by the air flow in this state, but the passing area can be reduced, but if the inner tube 122 is contracted inwards of the outer tube 121, the blocking surface can press the flexible seat 123, so that the inner channel is expanded, the radius is increased, the area is increased, and the effect of increasing the pressure of the air flow is eliminated.

In this embodiment, the tube 121 and the base 113 are integrally connected.

The following principles are derived for the cooperation of the above structures:

firstly, the device is taken to a designated position, preferably, the device is held by the holding handle 220, then the motor is powered on, at the moment, the output shaft of the motor drives the fan blades 1111 to rotate, the rotating fan blades 1111 enable air in the environment to enter the chamber, airflow is formed in the air entering chamber and flows into the middle chamber, meanwhile, one hand which does not hold the holding handle 220 holds the outer tube 121 and the outer wall of the base 113, the two hands exert force in opposite directions, the inner tube 122 is contracted into the outer tube 121 along the sliding opening 121A, thus, the inner channel of the flexible seat 123 is expanded under the action of the blocking surface, the radius of the expanded channel is matched with the radius of the corresponding air flow channel (mainly the part which is communicated with the air in the inner tube 122), and therefore, the large-area entry of the air is facilitated, and the sample preparation of the sample preparation member 130 is facilitated;

the sample preparation structure of the sample preparation member 130 is as shown in fig. 10, the sample preparation member 130 includes a sample preparation seat 131 and a sample preparation carrier plate 132, an air inlet channel 131A is penetratingly disposed on the sample preparation seat 131, the air inlet channel 131A forms the last part of the air flow channel, and a sample preparation chamber 131B accommodating the sample preparation carrier plate 132 is disposed on a side surface of the sample preparation seat 131, preferably, the sample preparation chamber 131B is disposed on the same side as the display end 230, so that the sample preparation seat 131 does not need to be rotated back and forth when the sample preparation carrier plate 132 is replaced or digital information on the display end 230 is observed, convenience of operation is improved, unnecessary steps are saved, as shown in fig. 10 continuously, a carrier groove 132A for carrying a sample preparation surface is disposed on the top of the sample preparation carrier plate 132, and a notch 132B is disposed on the bottom of the carrier groove 132A;

in this embodiment, the main purpose is to realize integration of sampling and detection, so a round flocked swab or a cotton swab is placed in the carrying groove 132A, which is different from the ordinary swab, because the ordinary swab is used for actively collecting a sample, an overlarge contact surface is not needed, but the invention needs the swab to passively collect a sample, namely, microorganisms in the air, so the swab needs to be round and flatly placed in the carrying groove 132A, and then the round swab is used for trapping the microorganisms in the air flow channel outflow air;

and in order to crack the microorganism, the round swab is immersed in the lysis solution (before the round swab is placed in the loading groove 132A, the swab is immersed in the lysis solution for 2-3 min), so that the microorganism trapped by the round swab is cracked by the lysis solution in the round swab to release ATP, after 1-2min, the time is firstly to ensure sufficient air inflow and secondly to provide time for cracking, after 1-2min, the hand holding the outer wall of the outer tube 121 and the outer wall of the base 113 is released, the inner tube 122 is reset under the self elastic force of the flexible seat 123, namely, extends out of the outer tube 121, the radius of the channel is reduced at this time, the pressure of the air flowing through the channel is increased, the air flow after the pressure is increased acts on the round swab, and the round swab is acted to slide in the notch 132B, the round swab sliding into the notch 132B enters the sampling groove 211A, the detection head 211 required to extend into the air flow channel is located at the bottom of the notch 132B, after the round swab enters the sampling groove 211A, released ATP is mixed with enzyme reaction liquid in the detection end 210 to generate reaction luminescence, the detection end 210 can read the reflection value of the reflection value at this time, the number of ATP is obtained through the luminescence value, then the content of microorganisms in the round swab can be obtained, digital signals are output through the display end 230, only 10-15s are needed in the whole process, so that the detection mode is very efficient, and the problem that the microorganisms in the air cannot be directly sampled by the swab is solved through the integrated sampling and detection.

In practical use, the above principle brings the following advantages:

inhale the air through air intake component 110 in to the air flow channel, form the air current, inhale the component 110 through the air and carry out initiative sampling like this, and the circular swab is used for intercepting the microorganism in the air current, just so need not to carry out initiative sampling through the circular swab, the air that mainly cooperates air intake component 110 to inhale carries out the preparation of sample can, thereby for detecting body 200's detection provides the sample, realize the integration of sampling and detection, the slow problem of detection efficiency after solving the flat dish sampling promptly, solve the problem that the swab can not directly sample to the microorganism in the air again.

Because of the formation of the air flow, the state of the air flow can be changed by the expansion and contraction of the intermediate member 120, and the sampling area needs to be enlarged when preparing a sample, so the radius of the air flow channel is enlarged by the extension of the intermediate member 120, and when detecting, the radius of the air flow channel is reduced by the contraction of the intermediate member 120, the air flow pressure is increased, the round swab is pressed into the detecting body 200 for detection, and the air flow is controlled and utilized in the whole process, so that the condition that the swab is manually contacted is avoided.

In the actual use process, the detection of the content of microorganisms in the air usually needs real-time detection, because the content of the microorganisms in the air is mostly detected after environmental disinfection, the result needs to be rapidly obtained, and the position change of the detection point is frequent, so that the sampling and detection integrated device meets the actual requirement.

In addition, in order to facilitate the extraction of the sample preparation carrier plate 132, a handle 1322 is disposed on the outer sidewall of the sample preparation carrier plate 132 to provide a point of application for applying force to the hand, and an outer frame 1321 is disposed on the outer periphery of the outer side of the sample preparation carrier plate 132, and the outer frame 1321 is a magnetic attraction structure, that is, an area attracted to the outer frame 1321 is disposed on the sample preparation seat 131 at the periphery of the sample preparation chamber 131B, so that after the sample preparation carrier plate 132 is inserted into the sample preparation chamber 131B, the outer frame 1321 is attached to the area, the sample preparation carrier plate 132 is positioned by using the magnetic attraction force to prevent the sample preparation carrier plate 132 from falling off in the using process, and the extraction is performed by applying an external force to overcome the magnetic attraction force.

It should be noted that in the present embodiment, the connection manner of the sample preparation carrier plate 132 and the sample preparation chamber 131B is not limited to the magnetic attraction connection, and may be a bolt connection or a snap connection, which is not necessarily described herein.

Figures 2 and 3 show a second embodiment of the invention,

different from the first embodiment, the detecting end 210 and the sample preparation member 130 are detachably connected, specifically, as shown in fig. 3, a suction cup 212 is disposed outside the detecting head 211, the suction cup 212 is of a magnetic structure, and as the principle of the outer frame 1321, an area which is attracted by the suction cup 212 is disposed at a position corresponding to the attachment position of the suction cup 212 at the bottom of the sample preparation member 130 (i.e., at the bottom of the sample preparation seat 131), so as to realize the detachable connection therebetween, thereby facilitating the replacement of the sampling body 100 or the detecting body 200.

Furthermore, the detached sample body 100 and the specimen 200 can perform independent operations:

the detection body 200 is obtained by directly sampling a sample with a swab shaped like a stick and then inserting the swab into the sampling groove 211A for detection, and is commonly used for detection of a liquid sample;

the sampling body 100 realizes sampling and does not perform detection, at this time, a round swab is not placed in the loading groove 132A, but a plate, air is sucked by the fan blade 1111, and the air channel impacts the plate to realize sampling of microorganisms.

Figure 9 shows a third embodiment of the invention,

different from the first embodiment, the outer tube 121 and the base 113 are of a split structure, so that an air flow channel inside the outer tube 121 is convenient to clean, specifically, an outer ring plate 1211 is arranged on the periphery of the joint of the base 113 and the outer tube 121, the outer ring plate 1211 is used for fixing the base 113 and the outer tube 121, the base 113 and the outer tube 121 can be clasped by clamping and clamping, the periphery of the outer ring plate 1211 protrudes outwards, and a force bearing surface is formed for the hands to exert force, and the arrangement is more in accordance with the human body.

Figures 6, 7, 11 and 12 show a fourth embodiment of the invention,

as shown in fig. 6, in the embodiment, the base 113 is provided with the flow guide seat 1131, the bottom of the flow guide seat 1131 is provided with an opening, and the inner wall of the flow guide seat 1131 is contracted towards the opening, so that the radius of the air flow channel is reduced while large-area air absorption is realized, and the size of the subsequent sampling slot 211A and the size of the notch 132B can be adapted, and the air flow is also concentrated;

in addition, the central seat 112 is a columnar structure with a thick middle part and thin two ends, an inner receiving surface 112a and an outer expanding surface 112b are formed outside the central seat 112 through the structure, and a support plate 114 is arranged along the extending direction of the outer expanding surface 112b and fixedly connected with the drainage seat 1131, so that the central seat 112 is supported, as shown in fig. 7, a through groove 114A is arranged at the bottom of the support plate 114, the wind resistance is reduced as much as possible along the outer expanding surface 112b, the support plate 114 is also arranged into an arc-shaped structure on the basis, in addition, a drainage cavity channel 110A is formed between the inner receiving surface 112a and the drainage seat 1131, and the airflow flows into the air channel of the subsequent part through the drainage cavity channel 110A and the through groove 114A.

In this embodiment, the bottom surface of the drainage seat 1131 forms a blocking surface, as shown in fig. 11, the flexible seat 123 is composed of a force-receiving portion 123a and a supporting portion 123b, the force-receiving portion 123a is disposed on the top of the supporting portion 123b, as shown in fig. 12, and a "V" shaped structure is formed therebetween to reduce the flow radius of the channel through the protruding portion in the normal state.

In fig. 11, the inner tube 122 is retracted into the outer tube 121, and the force receiving portion 123a is compressed by the obstruction of the drainage seat 1131, and the protruding portion is expanded, so that the passage flow radius is increased.

In fig. 12, the force receiving portion 123a is reset by its own elastic force, and then the inner tube 122 is ejected, and the outer peripheral radius of the supporting portion 123b is larger than the outer diameter of the inner tube 122, which can play a role of a restriction to prevent the inner tube 122 from coming off the slide port 121A.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. An air microorganism sampling and detecting integrated device, which comprises a sampling body (100), wherein the sampling body (100) comprises an air suction component (110) forming an air flow channel, an intermediate component (120) and a sample preparation component (130), wherein the air suction component (110) is arranged at the top of the intermediate component (120), the sample preparation component (130) is arranged at the bottom of the intermediate component (120), air sucked by the air suction component (110) enters the sample preparation component (130) through the air flow channel for sample preparation, and the air microorganism sampling and detecting integrated device is characterized in that: the sampling device further comprises a detection body (200) arranged at the bottom of the sampling body (100), the intermediate member (120) is of a telescopic structure, and the flow radius of an air flow channel is changed through the telescopic structure of the intermediate member (120), wherein:

during sampling, the intermediate component (120) contracts, and the radius of an air flow channel is increased;

after sample preparation, the intermediate member (120) extends outwards, the radius of an air flow channel is reduced, the pressure of air forming airflow is increased, and the sample obtained by sample preparation is pressed to enter the detection body (200) for detection.

2. The integrated device for sampling and detecting airborne microorganisms according to claim 1, wherein: the detection body (200) comprises a detection end (210) and a display end (230), wherein the display end (230) is arranged on one side of the detection end (210) and is used for displaying digital information detected by the detection end (210);

the top of the detection end (210) is provided with a detection head (211), the top of the detection head (211) is provided with a sampling groove (211A), and the detection head (211) connected with the sample preparation component (130) extends into an air flow channel in the sample preparation component (130).

3. The integrated sampling and detecting device for airborne microorganisms according to claim 2, wherein: the detection head (211) is integrally connected with the sample preparation member (130).

4. The integrated sampling and detecting device for airborne microorganisms according to claim 1, wherein: the air suction member (110) comprises a top cover (111) and a base (113) arranged at the bottom of the top cover (111), and the top cover (111) and the base (113) are connected to form an air inlet chamber;

a fan blade (1111) is rotationally connected in the air inlet chamber;

a central seat (112) fixed on a base (113) is arranged in the air inlet chamber, and a motor for driving fan blades (1111) to rotate is arranged on the central seat (112).

5. The integrated device for sampling and detecting airborne microorganisms according to claim 4, wherein: the middle member (120) comprises an outer pipe (121) and an inner pipe (122), a middle chamber is formed between the outer pipe (121) and the inner pipe (122), a sliding opening (121A) connected with the inner pipe (122) in a sliding mode is formed in the bottom of the outer pipe (121), and the outer pipe (121) and the inner pipe (122) are connected in a sliding mode to form a telescopic structure;

a flexible seat (123) is arranged at the top of the inner pipe (122), and a blocking surface is arranged above the flexible seat (123) in the middle cavity;

the flexible seat (123) is an annular structure having a channel therein, wherein: in the normal state of the flexible seat (123), the radius of the channel is smaller than that of the air flow channel; when the inner pipe (122) shrinks towards the inside of the outer pipe (121), the blocking surface presses the flexible seat (123) to expand the internal channel of the flexible seat (123).

6. The integrated sampling and detecting device for airborne microorganisms according to claim 5, wherein: the outer tube (121) and the base (113) are integrally connected.

7. The integrated device for sampling and detecting airborne microorganisms according to claim 1, wherein: the sample preparation component (130) comprises a sample preparation seat (131) and a sample preparation carrier plate (132), wherein an air inlet channel (131A) penetrates through the sample preparation seat (131);

a sample preparation cavity (131B) for accommodating a sample preparation carrier plate (132) is formed in the side surface of the sample preparation seat (131), and a sample preparation groove (132A) for bearing a sample preparation surface is formed in the top of the sample preparation carrier plate (132);

the bottom of the carrying groove (132A) is provided with a notch (132B).

8. The integrated device for sampling and detecting airborne microorganisms according to claim 2, wherein: the detection end (210) and the sample preparation member (130) are detachably connected.

9. The integrated device for sampling and detecting airborne microorganisms according to claim 5, wherein: the outer tube (121) and the base (113) are of a split structure, an outer ring plate (1211) is arranged on the periphery of the joint of the base (113) and the outer tube (121), the outer ring plate (1211) is used for fixing the outer ring plate and the outer tube, and the periphery of the outer ring plate (1211) protrudes outwards.

10. The integrated device for sampling and detecting airborne microorganisms according to claim 5, wherein: the flexible seat (123) is composed of a force bearing part (123 a) and a supporting part (123 b), the force bearing part (123 a) is arranged at the top of the supporting part (123 b), and a V-shaped structure is formed between the force bearing part (123 a) and the supporting part (123 b).