CN116855557A - Preparation method of agricultural glucan and fertilizer thereof - Google Patents
Preparation method of agricultural glucan and fertilizer thereof Download PDFInfo
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- CN116855557A CN116855557A CN202310678178.0A CN202310678178A CN116855557A CN 116855557 A CN116855557 A CN 116855557A CN 202310678178 A CN202310678178 A CN 202310678178A CN 116855557 A CN116855557 A CN 116855557A
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- 229920001503 Glucan Polymers 0.000 title claims abstract description 110
- 239000003337 fertilizer Substances 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title abstract description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 59
- 108090000790 Enzymes Proteins 0.000 claims abstract description 59
- 239000007788 liquid Substances 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 210000005253 yeast cell Anatomy 0.000 claims abstract description 29
- 230000009849 deactivation Effects 0.000 claims abstract description 20
- 239000000843 powder Substances 0.000 claims description 19
- 238000000265 homogenisation Methods 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 108091005658 Basic proteases Proteins 0.000 claims description 10
- 101710130006 Beta-glucanase Proteins 0.000 claims description 9
- 108010012864 alpha-Mannosidase Proteins 0.000 claims description 8
- 102000019199 alpha-Mannosidase Human genes 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 6
- 230000000415 inactivating effect Effects 0.000 claims description 6
- 102100023164 Epididymis-specific alpha-mannosidase Human genes 0.000 claims description 4
- 101000979031 Homo sapiens Epididymis-specific alpha-mannosidase Proteins 0.000 claims description 4
- 102100021771 Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase Human genes 0.000 claims description 3
- 108010009689 mannosyl-oligosaccharide 1,2-alpha-mannosidase Proteins 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims 5
- 230000000694 effects Effects 0.000 abstract description 5
- 229940088598 enzyme Drugs 0.000 description 47
- 230000000052 comparative effect Effects 0.000 description 15
- 238000000605 extraction Methods 0.000 description 11
- 229920002307 Dextran Polymers 0.000 description 8
- 229920000057 Mannan Polymers 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- 244000061456 Solanum tuberosum Species 0.000 description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 description 5
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 4
- 229920002498 Beta-glucan Polymers 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000003895 organic fertilizer Substances 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000000413 hydrolysate Substances 0.000 description 3
- 235000012015 potatoes Nutrition 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 235000015872 dietary supplement Nutrition 0.000 description 2
- LUEWUZLMQUOBSB-GFVSVBBRSA-N mannan Chemical class O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@H]3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-GFVSVBBRSA-N 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102100032487 Beta-mannosidase Human genes 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 108010055059 beta-Mannosidase Proteins 0.000 description 1
- 238000010000 carbonizing Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Polymers 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P19/08—Dextran
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- Chemical & Material Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
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- Enzymes And Modification Thereof (AREA)
Abstract
The application relates to a preparation method of agricultural glucan and a fertilizer thereof, which comprises the steps of 1) carrying out enzymolysis on yeast cell walls in water, separating to obtain enzymolysis liquid and residues after enzyme deactivation, 2) adding the residues into the water for homogenizing, adding enzyme for secondary enzymolysis, and separating to obtain glucan liquid and residues 2 after enzyme deactivation. The secondary enzymolysis liquid obtained by the application has higher glucan content and good water solubility, and has better effect when being applied to agriculture.
Description
Technical Field
The application relates to a preparation method of agricultural glucan and a fertilizer thereof.
Background
The beta-glucan can effectively regulate the immune function of the organism and promote the generation of IgM antibodies in the body, so that the beta-glucan can be widely applied to foods and can improve the immunity of human bodies and animals.
At present, the technology for preparing beta-glucan by enzymatic enzymolysis of yeast cell walls is mature, such as the preparation of beta-glucan and mannan with the patent name of CN 101184780A, which adopts alkaline protease to carry out enzymolysis on the yeast cell walls to obtain a glucan-rich component and a mannan-rich component; or after enzymatic hydrolysis of the yeast cell wall with alkaline protease, further selecting at least one enzyme of amylase, lipase and combinations thereof to incubate the protease treated cell wall of step (b) for use as a food supplement, pharmaceutical, cosmetic or nutritional supplement; or, animal feeds, are not used in agriculture.
The patent name of the publication No. CN 109207384A is modified yeast cell wall, and a preparation method and application thereof, which discloses that the yeast cell wall milk is subjected to enzymolysis by mannanase, alkaline protease, papain, cellulase and beta-glucanase, and dried to obtain the yeast cell wall. It is applied in the field of feed additives.
Dextran has been widely used in humans and animals, but has been reported for plants, however, dextran has more requirements in agriculture, such as stable quality and better promotion of crop growth.
Currently, there are few agricultural glucans, especially fertilizers, and there is a need in the market for agricultural glucan that can be adapted to agriculture.
Disclosure of Invention
The application provides a preparation method of agricultural glucan and a fertilizer thereof, which solves the technical problems that 1) the extraction rate of the agricultural glucan is improved; 2) Preparing glucan suitable for agriculture, wherein the glucan can promote crop growth; 3) The quality of the prepared agricultural glucan is kept stable.
In order to solve the technical problems, the application adopts the following technical scheme:
the preparation method of the agricultural glucan comprises the following steps of
1) Performing enzymolysis on yeast cell walls in water, and separating to obtain enzymolysis liquid and residues after enzyme deactivation;
2) Adding the residues obtained in the step 1) into water, homogenizing, adding enzyme for secondary enzymolysis, inactivating enzyme, and separating to obtain agricultural glucan liquid and residues 2;
the enzyme for enzymolysis in step 1) is alkaline protease;
the enzyme added in step 2) by enzymolysis is beta-glucanase.
The step 2) further comprises adding an alpha mannosidase.
The alpha mannosidase is one or two of alpha-1, 3-mannosidase, alpha-1, 2-mannosidase or alpha-1, 6-mannosidase.
The mass ratio of the yeast cell wall to the enzyme to the water in the step 1) is 7-20:0.001-0.006:80-92.999;
the enzymolysis condition is pH 8.5-11, temperature 40-55deg.C, time 8-24 h; the enzyme deactivation condition is that the temperature is 90-100 ℃ and the time is 1-3 min.
The homogenization condition in the step 2) is that the pressure is 4-10 Mpa and the temperature is 50-70 ℃.
The mass ratio of the residues, the enzyme and the water in the step 2) is 7-20:0.001-0.006:80-92.999;
the enzymolysis condition is pH 4.5-6, temperature 40-55 deg.C, time 8-24 h; the enzyme deactivation condition is that the temperature is 90-100 ℃ and the time is 1-3 min.
The method also comprises the step of drying the glucan liquid to obtain glucan powder for agriculture;
the drying temperature is not more than 85 ℃.
The agricultural glucan is directly used as fertilizer, or
Adding agricultural glucan into fertilizer, and using together with the fertilizer;
the application has the following beneficial technical effects:
1) The application further adds glucanase to hydrolyze the glucan, thereby preparing the glucan suitable for agriculture, and compared with the glucan only subjected to enzymolysis by the protease, the glucan for agriculture can promote the growth of crops more obviously.
2) The application adopts the conditions that the pressure is 4-10 Mpa and the temperature is 50-70 ℃ to homogenize the residue, and the effect is to change the spiral structure of the glucan, because the glucan has a plurality of hydroxyl groups in the molecule, three polysaccharide chains are arranged and wound in parallel due to the interaction of hydrogen bonds, a compact triple helix is formed, and the triple helix is rotated into a single chain shape through homogenization, so that the content of glucan in the glucan liquid for agriculture is improved.
3) The application needs to separate the enzymolysis liquid after the enzymolysis of the alkaline protease, and then carries out the enzymolysis of the glucanase, if the enzymolysis liquid is not separated, the prepared agricultural glucan liquid has low glucan content and has the problem of color change in the storage process, namely the quality of the product is unstable.
4) The application dries the glucan, limits the drying temperature not to exceed 85 ℃, and prevents the glucan from carbonizing in the drying process, thereby affecting the application effect of the glucan in agriculture.
Detailed Description
In order to better understand the above technical solution, the following describes the technical solution of the present application in detail with reference to a preferred example.
Example 1
The preparation method of the agricultural glucan comprises the following steps of
1) Performing enzymolysis on yeast cell walls in water, and separating to obtain enzymolysis liquid and residues after enzyme deactivation;
2) Adding all residues obtained in the step 1) into water, homogenizing, adding enzyme for secondary enzymolysis, inactivating enzyme, and separating to obtain agricultural glucan liquid and residues 2;
the enzyme for enzymolysis in step 1) is alkaline protease;
the enzyme added in the step 2) is beta-glucanase, and is a combination of beta-1, 6-glycosidase and beta-1, 3-glycosidase according to the mass ratio of 1:3.
The mass ratio of yeast cell wall, enzyme and water in step 1) was 8 (g): 0.001 (g): 91.999 (g);
the enzymolysis condition is pH10, temperature 50 ℃ and time 12h; the enzyme deactivation condition is that the temperature is 95 ℃ and the time is 2min.
The homogenization conditions in step 2) are a pressure of 6Mpa and a temperature of 60 ℃.
The mass ratio of residues, enzyme and water in the step 2) is 8:0.001:91.999;
the enzymolysis condition is pH5, temperature 45 ℃ and time 12h; the enzyme deactivation condition is that the temperature is 95 ℃ and the time is 2min.
The dextran solution is dried in a blast oven at 60 ℃ to obtain agricultural dextran powder, and the mass of the agricultural dextran powder is 2.47g;
the total glucan content of the agricultural glucan powder is detected by adopting a GOPOD method, the total glucan content is 2.27g, the total glucan accounts for 28.4% of the mass of the yeast cell wall, and the extraction rate is 96.0%.
Example 2
The preparation method of the agricultural glucan comprises the following steps of
1) Performing enzymolysis on yeast cell walls in water, and separating to obtain enzymolysis liquid and residues after enzyme deactivation;
2) Adding all residues obtained in the step 1) into water, homogenizing, adding enzyme for secondary enzymolysis, inactivating enzyme, and separating to obtain agricultural glucan liquid and residues 2;
the enzyme for enzymolysis in step 1) is alkaline protease;
the enzyme added in the step 2) is a combination of beta-glucanase and alpha mannosidase according to a mass ratio of 1:1.
The beta-glucanase is a composition of beta-1, 6-glycosidase and beta-1, 3-glycosidase according to a mass ratio of 1:3;
the alpha-mannosidase is a composition of alpha-1, 6-mannosidase and alpha-1, 3-mannosidase according to a mass ratio of 1:1;
the mass ratio of yeast cell wall, enzyme and water in step 1) was 8 (g): 0.001 (g): 91.999 (g);
the enzymolysis condition is pH10, temperature 50 ℃ and time 12h; the enzyme deactivation condition is that the temperature is 95 ℃ and the time is 2min.
The homogenization conditions in step 2) are a pressure of 6Mpa and a temperature of 60 ℃.
The mass ratio of residues, enzyme and water in the step 2) is 8:0.002:91.998;
the enzymolysis condition is pH5, temperature 45 ℃ and time 12h; the enzyme deactivation condition is that the temperature is 95 ℃ and the time is 2min.
The glucan liquid is dried in a blast oven at 60 ℃ to obtain agricultural glucan powder, and the mass of the agricultural glucan powder is 4.65g;
detecting the total glucan content and the total mannan content of the agricultural glucan powder by adopting a GOPOD method, wherein the total glucan content is 2.28g, the total mannan content is 1.76g, the total glucan mass accounts for 28.5% of the yeast cell wall mass, and the extraction rate is 96.3%; the total mass of mannans accounts for 22.0% of the mass of the yeast cell wall, and the extraction rate is 71.4%.
Example 3
The preparation method of the agricultural glucan comprises the following steps of
1) Performing enzymolysis on yeast cell walls in water, and separating to obtain enzymolysis liquid and residues after enzyme deactivation;
2) Adding all residues obtained in the step 1) into water, homogenizing, adding enzyme for secondary enzymolysis, inactivating enzyme, and separating to obtain agricultural glucan liquid and residues 2;
the enzyme for enzymolysis in step 1) is alkaline protease;
the enzyme added in the enzymolysis in the step 2) is beta-glucanase, and the enzyme consists of beta-1, 6-glycosidase and beta-1, 3-glycosidase according to the mass ratio of 1:2.
The mass ratio of yeast cell wall, enzyme and water in step 1) was 12 (g): 0.002 (g): 87.998 (g);
the enzymolysis condition is pH9, temperature 55 ℃ and time 14h; the enzyme deactivation condition is that the temperature is 98 ℃ and the time is 2min.
The homogenization conditions in step 2) are 5Mpa and 65 ℃.
The mass ratio of residues, enzyme and water in the step 2) is 12:0.002:87.998;
the enzymolysis condition is pH5.2, temperature is 47 ℃ and time is 13h; the enzyme deactivation condition is that the temperature is 95 ℃ and the time is 2min.
The glucan liquid is dried in a blast oven at 60 ℃ to obtain agricultural glucan powder, and the mass of the agricultural glucan powder is 3.51g;
the total glucan content of the agricultural glucan powder is detected by adopting a GOPOD method, the total glucan content is 3.40g, the total glucan accounts for 28.3 percent of the mass of the yeast cell wall, and the extraction rate is 95.6 percent.
Example 4
The preparation method of the agricultural glucan comprises the following steps of
1) Performing enzymolysis on yeast cell walls in water, and separating to obtain enzymolysis liquid and residues after enzyme deactivation;
2) Adding all residues obtained in the step 1) into water, homogenizing, adding enzyme for secondary enzymolysis, inactivating enzyme, and separating to obtain agricultural glucan solution and residues 2;
the enzyme for enzymolysis in step 1) is alkaline protease;
the enzyme added in the step 2) is a combination of beta-glucanase and alpha mannosidase according to a mass ratio of 1:1.
The beta-glucanase is a composition of beta-1, 6-glycosidase and beta-1, 3-glycosidase according to a mass ratio of 1:1;
the alpha-mannosidase is a composition of alpha-1, 6-mannosidase, alpha-1, 3-mannosidase and alpha-1, 2-mannosidase according to a mass ratio of 2:1:1;
the mass ratio of yeast cell wall, enzyme and water in step 1) was 10 (g) 0.002 (g): 89.998 (g);
the enzymolysis condition is pH10.5, temperature 50 ℃ and time 10h; the enzyme deactivation condition is that the temperature is 100 ℃ and the time is 1min.
The homogenization conditions in step 2) are a pressure of 8Mpa and a temperature of 55 ℃.
The mass ratio of residues, enzyme and water in the step 2) is 12:0.002:87.998;
the enzymolysis condition is pH5.2, the temperature is 51 ℃ and the time is 12 hours; the enzyme deactivation condition is that the temperature is 98 ℃ and the time is 2min.
The dextran solution is dried in a vacuum blast oven with the temperature of 50 ℃ and the pressure of 0.7MPa to obtain the agricultural dextran powder, and the mass of the agricultural dextran powder is 6.27g;
detecting the total glucan content of the agricultural glucan powder by adopting a GOPOD method, wherein the total glucan content is 2.88g, the total mannan content is 3.01g, the total glucan mass accounts for 28.8% of the yeast cell wall mass, and the extraction rate is 97.3%; the total mass of mannans accounts for 30.1% of the mass of the yeast cell wall, and the extraction rate is 97.7%.
The total glucan content in the yeast cell wall used in the test was 29.6% and the total mannan content was 30.8%.
Example 5
The agricultural glucan liquid is used as fertilizer and applied to agriculture.
When the agricultural glucan solution is used as a foliar fertilizer, the agricultural glucan solution needs to be diluted by 8-15 times by adding water before use;
when the agricultural glucan solution is used as a water-soluble fertilizer to be applied to drip irrigation, the agricultural glucan solution needs to be diluted by 6-8 times by adding water before use.
Example 6
Adding agricultural glucan powder into a fertilizer to obtain a fertilizer containing agricultural glucan;
the fertilizer comprises one of compound fertilizer, water-soluble fertilizer, organic and inorganic fertilizer and organic fertilizer.
Example 7
Adding agricultural glucan liquid into a fertilizer to obtain a fertilizer containing agricultural glucan;
the fertilizer comprises one of compound fertilizer, water-soluble fertilizer, organic and inorganic fertilizer and organic fertilizer.
Comparative example 1 (the same procedure as in example 1 was followed except that homogenization was not carried out), which produced 2.32g of agricultural glucan powder, 2.11g of total glucan content, 26.4% of yeast cell wall mass, and 89.2% of extraction yield;
comparative example 2 (except that the enzymatic hydrolysate prepared in step 1 was not separated, the other preparation methods were the same as in example 1) except that the mass of the agricultural glucan powder prepared was 2.35g, the total glucan content was 2.16g, and the extraction yield was 92.6% based on 27.4% of the mass of the yeast cell wall.
Comparative example 3 (the enzymatic hydrolysate prepared in step 1) was not separated nor homogenized, and the other preparation methods were the same as in example 1) except that the mass of the agricultural glucan powder prepared was 2.32g, the total glucan content was 2.10g, 26.3% of the mass of the yeast cell wall, and the extraction rate was 89.0%.
The agricultural glucan solutions prepared in examples 1 to 4, comparative example 1 and comparative example 2 were poured into 1L polyethylene bottles, stored for one month and three months in a sealed state, and observed for color change, wherein the color was just produced, as shown in Table 1
Note that: the enzymatic hydrolysate separated in comparative example 1 and examples 1 to 4 was also stored in a sealed condition, and no significant color change was observed over a period of three months.
From the data of the extraction rates of glucan of example 1, comparative example 1 and comparative example 2 and the color change during storage of the agricultural glucan liquid, it was found that the homogenization can increase the content of water-soluble glucan in the agricultural glucan liquid, whereas from comparative example 2, it was found that the homogenization can increase the content of glucan in the agricultural glucan liquid, but it had a problem that the color change occurred during storage, which affected the quality of the product. In order to clarify the cause of the color change, the applicant conducted the test of comparative example 3, which had a color change as well, but the color change was significantly retarded, and thus, the applicant considered that the temperature and pressure during the homogenization process accelerated the color change.
The problem of color change in the storage process of the agricultural glucan liquid can be effectively solved by separating the enzymolysis liquid prepared in the step 1).
The beneficial effects of the application are further illustrated below in conjunction with experimental data:
test material
1.1 test sites: the city of Fangshi is Anchu city of Fangzhi Zhenzhi.
1.2 experimental detection: potato leaf SPAD (chlorophyll content), including pre-treatment and post-treatment, averaged;
1.3 test method: selecting 4 cells, planting 30 potatoes in each cell, and respectively testing the glucan liquid for agriculture prepared in comparative example 1 (except that homogenization is not performed, the other preparation methods are the same as in example 1), the glucan liquid for agriculture prepared in example 1 and example 2 and the residues prepared in comparative example 5 (step 1 of example 1), wherein the glucan liquid for agriculture is prepared by diluting the glucan liquid for agriculture by 10 times with water, spraying the leaves of the potatoes with a spray can, and spraying 1L of diluted glucan liquid for agriculture in each cell; comparative example 5 (residue prepared in step 1) of example 1) was applied by hole application by applying 1kg of residue to the root of the potato under test at an average hole application depth of 8cm.
1.4 detection method: and detecting by using a SPAD detector, and taking an average value.
The pretreatment refers to SPAD detection on the day of the test treatment with each treatment, and the post-treatment refers to SPAD detection on the 5 th day after each treatment.
The application is consistent with other implementations except for different treatments.
2 results and analysis
The average value of SPADs for each treatment is shown in table 2
As can be seen from comparison of the data of comparative example 5 (step 1 of example 1) in Table 2, which is carried out by hole application of 1kg of residue on average to the roots of the potatoes tested, with a hole application depth of 8cm, and example 1, the agricultural glucan prepared in example 1 of the present application has a good effect in agriculture.
As can be seen from comparison of comparative example 1 (except that homogenization was not performed, the other preparation methods were identical to those of example 1) and example 1, the agricultural glucan prepared by homogenized example 1 can promote crop growth better when applied to agriculture.
Claims (8)
1. A method for preparing agricultural glucan is characterized by comprising the following steps of
1) Performing enzymolysis on yeast cell walls in water, and separating to obtain enzymolysis liquid and residues after enzyme deactivation;
2) Adding the residues obtained in the step 1) into water, homogenizing, adding enzyme for secondary enzymolysis, inactivating enzyme, and separating to obtain agricultural glucan liquid and residues 2;
the enzyme for enzymolysis in step 1) is alkaline protease;
the enzyme added in step 2) by enzymolysis is beta-glucanase.
2. The method of preparing agricultural glucan according to claim 1 wherein step 2) further comprises adding an alpha mannosidase.
3. The method for producing agricultural glucan according to claim 2, wherein the α -mannosidase is one or two of α -1, 3-mannosidase, α -1, 2-mannosidase and α -1, 6-mannosidase.
4. The method for preparing agricultural glucan according to claim 1, wherein the mass ratio of yeast cell wall, enzyme and water in the step 1) is 7-20:0.001-0.006:80-92.999;
the enzymolysis condition is pH 8.5-11, temperature 40-55deg.C, time 8-24 h; the enzyme deactivation condition is that the temperature is 90-100 ℃ and the time is 1-3 min.
5. The method for producing agricultural glucan according to claim 1, wherein the homogenization conditions in step 2) are a pressure of 4 to 10Mpa and a temperature of 50 to 70 ℃.
6. The method for producing agricultural glucan according to claim 1, wherein the mass ratio of the residue, the enzyme and the water in the step 2) is 7 to 20:0.001 to 0.006:80 to 92.999;
the enzymolysis condition is pH 4.5-6, temperature 40-55 deg.C, time 8-24 h; the enzyme deactivation condition is that the temperature is 90-100 ℃ and the time is 1-3 min.
7. The method for producing an agricultural glucan according to any one of claims 1 to 6, further comprising drying the glucan liquid to obtain an agricultural glucan powder;
the drying temperature is not more than 85 ℃.
8. The method for producing a fertilizer containing agricultural glucan as claimed in any one of claims 1 to 7, wherein agricultural glucan is directly used as a fertilizer, or
Adding agricultural glucan into fertilizer, and using together with the fertilizer;
the agricultural glucan comprises one or two of agricultural glucan liquid and agricultural glucan powder.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310397536 | 2023-04-14 | ||
| CN2023103975360 | 2023-04-14 |
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| Publication Number | Publication Date |
|---|---|
| CN116855557A true CN116855557A (en) | 2023-10-10 |
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|---|---|---|---|
| CN202310678178.0A Pending CN116855557A (en) | 2023-04-14 | 2023-06-09 | Preparation method of agricultural glucan and fertilizer thereof |
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| Country | Link |
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| CN (1) | CN116855557A (en) |
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2023
- 2023-06-09 CN CN202310678178.0A patent/CN116855557A/en active Pending
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