CN116836775A - Microfluidic chip analyzer - Google Patents
Microfluidic chip analyzer Download PDFInfo
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- CN116836775A CN116836775A CN202310798165.7A CN202310798165A CN116836775A CN 116836775 A CN116836775 A CN 116836775A CN 202310798165 A CN202310798165 A CN 202310798165A CN 116836775 A CN116836775 A CN 116836775A
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- 238000000605 extraction Methods 0.000 claims abstract description 83
- 150000007523 nucleic acids Chemical class 0.000 claims description 34
- 102000039446 nucleic acids Human genes 0.000 claims description 34
- 108020004707 nucleic acids Proteins 0.000 claims description 34
- 239000007788 liquid Substances 0.000 claims description 25
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 14
- 230000003321 amplification Effects 0.000 claims description 13
- 230000005415 magnetization Effects 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 2
- 239000011324 bead Substances 0.000 abstract description 60
- 238000001514 detection method Methods 0.000 abstract description 16
- 238000009792 diffusion process Methods 0.000 abstract description 6
- 230000009471 action Effects 0.000 description 16
- 239000002699 waste material Substances 0.000 description 7
- 230000009089 cytolysis Effects 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 239000006166 lysate Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000004804 winding Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
The application discloses a microfluidic chip analyzer, which relates to the technical field of biomolecule detection and comprises a rack and a centrifugal rotating assembly arranged on the rack, wherein a rotating shaft of the centrifugal rotating assembly is connected with a tray to drive the tray to centrifugally rotate, a preset position on the tray is used for fixing a microfluidic chip, a magnetic piece positioned above a tray projection is also arranged on the rack, and the magnetic field range of the magnetic piece covers a preset area of the tray. The microfluidic chip analyzer provided by the application can realize the full diffusion of the magnetic beads in the information extraction chamber of the microfluidic chip, thereby improving the detection accuracy.
Description
Technical Field
The application relates to the technical field of biomolecule detection, in particular to a microfluidic chip analyzer.
Background
Microfluidic chips, also known as labs on chip, are a technique for manipulating fluids on a micrometer scale. This technology scales the basic functionality of chemical and biological laboratories onto a chip that is only a few square centimeters in size. Miniaturization, automation, integration and portability from sample processing to detection are achieved through the intersection of analytical chemistry, microelectromechanical processing, computers, electronics, material systems, and biology, medicine, etc. The microfluidic chip has the characteristics of flexible combination and large-scale integration of various unit technologies, so that a small amount of samples can obtain extremely large information quantity, and the microfluidic chip is more likely to surpass a single analysis function, thereby becoming an integral miniature multi-element operation platform. The microfluidic chip also has the advantages of small volume, less reagent consumption, high analysis speed, easy integration and the like, and provides a wide prospect for application in various fields such as biomedical research, drug synthesis screening, environmental monitoring, health quarantine and the like.
When the microfluidic chip is applied to nucleic acid detection, the microfluidic chip comprises a nucleic acid extraction chamber, an amplification chamber, a first temperature control air chamber and a second temperature control air chamber, wherein the amplification chamber and the first temperature control air chamber are respectively communicated with the nucleic acid extraction chamber, the second temperature control air chamber is communicated with the amplification chamber, the nucleic acid extraction chamber is used for accommodating magnetic beads, a sample and a lysate, nucleic acid in the sample is released under the action of the lysate, the released nucleic acid is captured by the magnetic beads, and then the nucleic acid is extracted under the action of centrifugal force and transferred to the amplification chamber for amplification. However, when the microfluidic chip centrifugally rotates under the action of centrifugal force, the magnetic beads are deposited at the bottom of the nucleic acid extraction chamber due to the fact that the magnetic beads have larger mass and cannot be diffused in the sample liquid, and because the magnetic beads are carriers for capturing the released nucleic acids, the released nucleic acids cannot be captured and detected in the sample liquid without the magnetic beads, so that the accuracy of the test is affected.
Disclosure of Invention
The application aims to provide a microfluidic chip analyzer which can realize the full diffusion of magnetic beads in a nucleic acid extraction chamber of a microfluidic chip, thereby improving the detection accuracy.
An aspect of the embodiment of the application provides a microfluidic chip analyzer, which comprises a rack and a centrifugal rotating assembly arranged on the rack, wherein a rotating shaft of the centrifugal rotating assembly is connected with a tray to drive the tray to centrifugally rotate, a preset position on the tray is used for fixing a microfluidic chip, a magnetic part positioned above the projection of the tray is also arranged on the rack, and the magnetic field range of the magnetic part covers a preset area of the tray.
As an implementation manner, the magnetic member is connected with the frame through the moving assembly, and the moving assembly drives the magnetic member to move along the projection direction so as to adjust the coverage range of the magnetic field of the magnetic member on the tray.
As an embodiment, the moving assembly is further provided with an adjusting member for adjusting a horizontal distance of the moving assembly relative to the rotating shaft on a rotation plane of the tray.
As an embodiment, the magnetic element comprises an electromagnet or a permanent magnet.
As an implementation manner, a groove is formed in the tray, the groove is matched with the shape of the microfluidic chip to form a preset position, and the projection of the magnetic piece is in the range of the information extraction chamber of the microfluidic chip.
As an embodiment, the length D of the magnetic member in the circumferential direction of the centrifugal rotation and the length L of the information extraction chamber in the circumferential direction of the centrifugal rotation satisfy the relation: 2L > D > 1/4L.
As an embodiment, the length C of the magnetic member in the radial direction of the centrifugal rotation and the length of the information extraction chamber in the radial direction of the centrifugal rotation satisfy the relation: 1.2H > C > 0.8H.
As an implementation manner, a plurality of elastic clamping pieces are further arranged at intervals on the outer edge of the groove, and the elastic clamping pieces are used for limiting the position of the microfluidic chip.
As an implementation manner, the electromagnet is arranged on the rack through a control component, the control component comprises a controller and a switch connected with the controller, and the switch is connected with the electromagnet to control magnetization of the electromagnet according to signals of the controller.
As an implementation manner, the microfluidic chip comprises a chip body, the information extraction chamber is arranged in the chip body, a temperature control air cavity and an amplification chamber which are respectively connected with the information extraction chamber are also arranged in the chip body, the temperature control air cavity is used for sucking liquid in the information extraction chamber, and the amplification chamber is used for amplifying nucleic acid.
The beneficial effects of the embodiment of the application include:
the application provides a microfluidic chip analyzer, which comprises a rack and a centrifugal rotating assembly arranged on the rack, wherein a rotating shaft of the centrifugal rotating assembly is connected with a tray to drive the tray to centrifugally rotate, when the centrifugal rotating assembly drives the tray to centrifugally rotate, the microfluidic chip at the preset position of the tray rotates along with the tray, the microfluidic chip comprises a chip body, an information extraction chamber is arranged in the chip body, magnetic beads are preset in the information extraction chamber, after sample liquid and lysate are injected into the information extraction chamber, nucleic acid in the sample liquid is released under the action of the lysate, the magnetic beads capture the released nucleic acid to realize the extraction of the released nucleic acid, a magnetic piece positioned above the projection of the tray is further arranged on the rack, the magnetic field range of the magnetic piece covers the preset area of the tray, when the microfluidic chip rotates along with the tray, the magnetic field in the information extraction chamber rotates along with the centrifugal rotation of the tray, the magnetic beads in the information extraction chamber have attractive force on the magnetic beads in the information extraction chamber, the magnetic beads move under the action of the magnetic field, when the information extraction chamber rotates to the outside the magnetic field range, the magnetic beads are more accurately contacted with the magnetic beads in the extraction chamber, and the magnetic beads are more accurately and more quickly released under the action of the centrifugation chamber.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic structural diagram of a microfluidic chip analyzer according to an embodiment of the present application;
fig. 2 is a schematic structural diagram of a microfluidic chip according to an embodiment of the present application.
Icon: 10-a microfluidic chip analyzer; 110-a frame; 120-centrifuging the rotating assembly; 130-a tray; 140-magnetic member; 150-a microfluidic chip; 151-an information extraction chamber; 152-a temperature-controlled air cavity; 153-amplification chamber.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. The components of the embodiments of the present application generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the application, as presented in the figures, is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present application, it should be noted that, directions or positional relationships indicated by terms such as "center", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or those that are conventionally put in place when the product of this application is used, are merely for convenience of describing the present application and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be construed as limiting the present application.
Furthermore, the terms "horizontal," "vertical," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present application, it should also be noted that, unless explicitly stated and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.
The microfluidic chip has the advantages of high analysis speed, less reagent consumption, easy integration and the like, and the nucleic acid information in the sample liquid can be detected by the microfluidic chip analyzer after the sample liquid is added into the information extraction chamber of the microfluidic chip.
The embodiment of the application provides a microfluidic chip analyzer 10, as shown in fig. 1, which comprises a rack 110 and a centrifugal rotating assembly 120 arranged on the rack 110, wherein a rotating shaft of the centrifugal rotating assembly 120 is connected with a tray 130 to drive the tray 130 to centrifugally rotate, a preset position on the tray 130 is used for fixing a microfluidic chip 150, a magnetic element 140 positioned above the projection of the tray 130 is also arranged on the rack 110, and the magnetic field range of the magnetic element 140 covers a preset area of the tray 130.
The microfluidic chip analyzer 10 provided by the embodiment of the application is used for detecting the nucleic acid information in the sample liquid in the microfluidic chip 150. When detecting nucleic acid information, the micro-fluidic chip 150 is fixedly arranged at a preset position on the tray 130, the micro-fluidic chip 150 comprises an information extraction chamber 151, micro-magnetic beads are preset in the information extraction chamber 151, when the detection is carried out, sample liquid and lysis liquid are injected into the information extraction chamber 151, nucleic acid in the sample liquid is released under the action of the lysis liquid, the magnetic beads adsorb the released nucleic acid, and waste liquid is discharged to realize the extraction of nucleic acid information, when the centrifugal rotating assembly 120 drives the micro-fluidic chip 150 to centrifugally rotate, the micro-fluidic chip 150 is positioned in a magnetic field range of the magnetic piece 140 when the information extraction chamber 151 rotates below the projection of the magnetic piece 140, the magnetic beads have certain magnetism, under the action of the magnetic piece 140, the magnetic beads move in a direction close to or far away from the magnetic piece 140, so that the movement of the magnetic beads in the information extraction chamber 151 is realized, when the information extraction chamber 151 rotates away from the position below the projection of the magnetic piece 140, the magnetic beads move to the bottom of the information extraction chamber 151 under the action of the centrifugal force, and when the centrifugal rotating assembly 120 drives the tray 130 to centrifugally rotate, the magnetic beads move in a direction close to the magnetic field of the magnetic piece 140, and the magnetic beads can move in a reciprocating direction of the magnetic piece 140, and the nucleic acid can be more accurately released, and the nucleic acid can be more easily and reciprocally moved in the direction of the magnetic bead can be more easily detected.
It should be noted that, since the preset position is used for fixing the microfluidic chip 150, and the preset area is used for when the microfluidic chip 150 rotates centrifugally, and the information extraction chamber 151 in the microfluidic chip 150 rotates directly under the projection of the magnetic element 140, the magnetic element 140 generates a magnetic field to attract the magnetic beads in the information extraction chamber 151, and the preset area corresponds to the area corresponding to the information extraction chamber 151 when the microfluidic chip 150 is disposed at the preset position, so the area of the preset area is smaller than the area of the preset position.
It should be noted that, when the information extraction chamber 151 rotates below the projection of the magnetic member 140, the magnetic beads are subjected to both centrifugal force and magnetic field magnetic force, so that the magnetic fields of the magnetic member 140 should be set larger in order to enable the magnetic beads to move in a direction approaching or separating from the magnetic member 140. The magnetic force of the magnetic field on the magnetic beads is related to the magnetic field strength, the distance between the magnetic element 140 and the microfluidic chip 150, the surface area of the magnetic element 140, the positional relationship between the magnetic element 140 and the information extraction chamber 151, and the rotation speed of the microfluidic chip 150, and the specific setting of the above influencing factors is not limited in the embodiment of the present application, as long as the magnetic field of the magnetic element 140 can attract the magnetic beads to move in the information extraction chamber 151, and a person skilled in the art can perform specific setting according to practical situations.
The microfluidic chip analyzer 10 provided by the application comprises a rack 110 and a centrifugal rotating assembly 120 arranged on the rack 110, wherein a rotating shaft of the centrifugal rotating assembly 120 is connected with a tray 130 to drive the tray 130 to centrifugally rotate, a preset position on the tray 130 is used for fixing a microfluidic chip 150, when the centrifugal rotating assembly 120 drives the tray 130 to rotate, the microfluidic chip 150 at the preset position of the tray 130 centrifugally rotates along with the tray 130, the microfluidic chip 150 comprises a chip body, an information extraction chamber 151 is arranged in the chip body, magnetic beads are preset in the information extraction chamber 151, after sample liquid and lysis liquid are injected into the information extraction chamber 151, nucleic acid in the sample liquid is released under the action of the lysis liquid, the nucleic acid released by the capture of the magnetic beads realizes the extraction of the nucleic acid, a magnetic piece 140 positioned above the projection of the tray 130 is also arranged on the rack 110, the magnetic field range of the magnetic piece 140 covers the preset area of the tray 130, when the microfluidic chip 150 centrifugally rotates along with the tray 130, the information extraction chamber 151 in the microfluidic chip 150 centrifugally rotates along with the magnetic field range of the magnetic piece 140, the magnetic beads are more accurately dispersed under the action of the magnetic beads, and the magnetic beads are more accurately dispersed under the action of the magnetic field of the magnetic beads in the information extraction chamber, and the magnetic beads are fully dispersed under the action of the magnetic field, and the magnetic beads are fully dispersed under the action of the magnetic beads in the magnetic field, and the magnetic field is fully extracted under the magnetic field under the action of the magnetic field.
Alternatively, the magnetic member 140 is connected to the frame 110 through a moving assembly, and the moving assembly drives the magnetic member 140 to move along the projection direction to adjust the coverage of the magnetic field of the magnetic member 140 on the tray 130.
The magnetic element 140 is arranged on the rack 110 through the moving assembly, the vertical distance between the magnetic element 140 and the tray 130 can be adjusted through adjusting the moving assembly, the coverage range of the magnetic field of the magnetic element 140 on the tray 130 is further adjusted, when the microfluidic chip 150 detects, the vertical distance between the magnetic element 140 and the tray 130 can be adjusted according to the occupied area of the information extraction chamber 151, when the distance between the magnetic element 140 and the tray 130 is closer, the larger the area of the magnetic field of the magnetic element 140 is, the larger the area of the information extraction chamber 151 can be covered, and when the distance between the magnetic element 140 and the tray 130 is farther, the smaller the area of the magnetic field of the magnetic element 140 is, the smaller the area of the information extraction chamber 151 can be covered.
In addition, the vertical distance between the magnetic member 140 and the tray 130 can be adjusted according to the time that the magnetic beads need to move, specifically, when the size of the information extraction chamber 151 is determined, the magnetic field of the magnetic member 140 covers a larger area when the magnetic member 140 is closer to the tray 130, and when the information extraction chamber 151 rotates along with the tray 130, the longer the information extraction chamber 151 is located in the magnetic field, the longer the acting time of the magnetic field on the magnetic beads is, the longer the distance that the magnetic beads move is, so that the diffusion degree of the magnetic beads in the information extraction chamber 151 can be further increased, more released nucleic acids can be captured, and the detection accuracy is improved. Because the distance between the information extraction chamber 151 in the microfluidic chip 150 along the vertical direction is limited, the distance between the magnetic beads moving is limited, the magnetic beads are attached to the side wall of the information extraction chamber 151 after moving to the side wall, and when the magnetic member 140 is closer to the tray 130, there is a risk of collision with the microfluidic chip 150. The position of the magnetic member 140 in the vertical direction can be adjusted by a moving assembly according to the actual circumstances by those skilled in the art.
In order to avoid the influence of the magnetic element 140 when the microfluidic chip 150 is removed from the tray 130 or placed on the tray 130 after the detection, the magnetic element 140 may be moved to a distance far from the tray 130 by using a moving assembly, and the magnetic element 140 may be moved to a distance near the tray 130 during the detection.
In one implementation manner of the embodiment of the present application, the moving component is further provided with an adjusting member, where the adjusting member is used to adjust the horizontal distance between the moving component and the rotating shaft on the rotating plane of the tray 130.
In order to enable the magnetic field of the magnetic element 140 to correspond to the position of the information extraction chamber 151 of each microfluidic chip 150, in the embodiment of the application, an adjusting element is further arranged on the moving component, and the adjusting element adjusts the horizontal distance between the moving component and the rotating shaft on the rotating plane of the tray 130 relative to the rotating shaft, namely, the adjusting element adjusts the distance between the moving component and the rotating shaft, so that the distance between the magnetic element 140 and the rotating shaft is adjustable, and when the microfluidic chip 150 is detected, the distance between the magnetic element 140 and the rotating shaft can be adjusted according to the position of the information extraction chamber 151 in the microfluidic chip 150, so that the microfluidic chip analyzer 10 can adapt to the detection of multiple microfluidic chips 150, and the applicability of the microfluidic chip analyzer 10 is improved.
Alternatively, the magnetic member 140 includes an electromagnet or a permanent magnet.
In one implementation manner of the embodiment of the present application, as shown in fig. 2, a groove is disposed on the tray 130, and the groove matches with the shape of the microfluidic chip 150 to form a preset position, and the projection of the magnetic element 140 is within the range of the information extraction chamber 151 of the microfluidic chip 150.
The microfluidic chip 150 is arranged in the groove, so that the microfluidic chip 150 can be prevented from moving relative to the tray 130 in the rotation process, and the stability of the position of the microfluidic chip 150 is improved.
Alternatively, the length D of the magnetic member 140 in the circumferential direction of the centrifugal rotation and the length L of the information extraction chamber 151 in the circumferential direction of the centrifugal rotation satisfy the relationship: 2L > D > 1/4L.
When the micro-fluidic chip 150 rotates along with the tray 130, the information extraction chamber 151 on the micro-fluidic chip 150 passes through the lower part of the magnetic piece 140 and is positioned in the magnetic field of the magnetic piece 140, wherein the longer the magnetic piece 140 is along the circumferential direction of centrifugal rotation, the longer the information extraction chamber 151 is positioned in the magnetic field, the longer the acting time of the magnetic field on the magnetic beads is, the longer the distance of the magnetic beads moves, the diffusion degree of the magnetic beads in the information extraction chamber 151 can be further increased, more released nucleic acids are captured, and the detection accuracy is improved. The length of the magnetic element 140 along the circumferential direction of the centrifugal rotation is too long, and because the distance of the information extraction chamber 151 in the microfluidic chip 150 along the vertical direction is limited, the distance of the movement of the magnetic beads is limited, the magnetic beads are attached to the side wall after moving to the side wall of the information extraction chamber 151, the effect of increasing the diffusion degree of the magnetic beads cannot be achieved, the volume of the magnetic element 140 is increased, and based on the consideration of the two aspects, the length D of the magnetic element 140 along the circumferential direction of the centrifugal rotation and the length L of the information extraction chamber 151 along the circumferential direction of the centrifugal rotation satisfy the following relation: 2L > D > 1/4L.
In one implementation manner of the embodiment of the present application, the length C of the magnetic element 140 along the radial direction of centrifugal rotation and the length of the information extraction chamber 151 along the radial direction of centrifugal rotation satisfy the following relation: 1.2H > C > 0.8H.
The length of the magnetic element 140 along the radial direction of centrifugal rotation is set to be equal to the length of the information extraction chamber 151 along the radial direction of centrifugal rotation, when the information extraction chamber 151 rotates to the minimum distance from the magnetic element 140, the magnetic field generated by the magnetic element 140 can cover the information extraction chamber 151, and the magnetic field attracts the magnetic beads in the information extraction chamber 151 to enable the magnetic beads to move.
The distance between the magnetic element 140 and the rotating shaft can be slightly smaller than the distance between the information extraction chamber 151 and the rotating shaft, so that the magnetic field is located at one side close to the rotating shaft, and then the magnetic beads can move towards the direction close to the rotating shaft under the action of the magnetic field, the diffusion of the magnetic beads in the information extraction chamber 151 is further improved, more released nucleic acids are obtained, and the detection accuracy is improved.
Optionally, a plurality of elastic clamping members are further disposed at intervals on the outer edges of the grooves, and the elastic clamping members are used for limiting the positions of the microfluidic chip 150.
The elastic clamping piece is used for limiting the position of the micro-fluidic chip 150, so that the micro-fluidic chip 150 is prevented from moving due to inertia in the rotation process or when the rotation speed changes, and the stable fixation of the micro-fluidic chip 150 is improved.
In one implementation of the embodiment of the present application, the electromagnet is disposed on the frame 110 through a control assembly, where the control assembly includes a controller and a switch connected to the controller, and the switch is connected to the electromagnet to control magnetization of the electromagnet according to a signal of the controller.
The electromagnet comprises an iron core and a conductive winding wound outside the iron core, the conductive winding is connected with a power supply through a switch, and the current passing through the conductive winding is controlled through the on state of the control switch, so that the magnetization degree of the electromagnet is controlled, namely the magnetic field intensity of a generated magnetic field is adjusted corresponding to different microfluidic chips 150 and different detection states, the microfluidic chip analyzer 10 can adapt to more conditions, and the applicability of the microfluidic chip analyzer 10 is improved.
Alternatively, as shown in fig. 2, the microfluidic chip 150 includes a chip body, the information extraction chamber 151 is disposed in the chip body, and a temperature control air chamber 152 and an amplification chamber 153 connected to the information extraction chamber 151 are further disposed in the chip body, respectively, the temperature control air chamber 152 is used for sucking the liquid in the information extraction chamber 151, and the amplification chamber 153 is used for amplifying the released nucleic acid.
The nucleic acid information is extracted in the information extraction chamber 151, after the nucleic acid released by the magnetic beads is adsorbed, the waste liquid in the information extraction chamber 151 is discharged into the temperature control air chamber 152, and the magnetic beads adsorbed with the released nucleic acid are transferred to the amplification chamber 153 for amplification and detection, so that the detection of the sample in the microfluidic chip 150 is completed. Among them, the discharge of the waste liquid in the information extraction chamber 151 to the temperature control air chamber 152 is in the following ways: first, the temperature control air cavity 152 is cooled, the compressible medium in the temperature control air cavity 152 contracts, so that the pressure in the temperature control air cavity 152 increases, and then waste liquid in the information extraction chamber 151 is sucked into the temperature control air cavity 152, in the process, as the bottom wall of the information extraction chamber 151 is rough, the microscopic state has an uneven surface state, friction exists between the magnetic beads deposited on the bottom and the bottom wall, and therefore the magnetic beads can be reserved on the bottom wall, separation of the magnetic beads and the waste liquid is achieved, and the waste liquid is discharged. Second, the temperature-controlled air chamber 152 is disposed at a side of the information extraction chamber 151 away from the rotation shaft, and the waste liquid is pressed into the temperature-controlled air chamber 152 by adjusting the rotation speed of the tray 130 and by rotational acceleration.
The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (10)
1. The utility model provides a micro-fluidic chip analyzer, its characterized in that, including the frame and set up in centrifugal rotatory subassembly in the frame, centrifugal rotatory subassembly's pivot is connected with the tray in order to drive the tray centrifugation rotates, preset position on the tray is used for fixed micro-fluidic chip, still be provided with in the frame and be located the magnetic part of tray projection top, the magnetic field scope of magnetic part covers the preset region of tray.
2. The microfluidic chip analyzer of claim 1, wherein the magnetic member is connected to the frame by a moving assembly that moves the magnetic member in a projection direction to adjust a coverage of a magnetic field of the magnetic member on the tray.
3. The microfluidic chip analyzer according to claim 2, wherein the moving assembly is further provided with an adjusting member for adjusting a horizontal distance of the moving assembly with respect to the rotation shaft on a rotation plane of the tray.
4. A microfluidic chip analyser according to any one of claims 1 to 3, wherein the magnetic element comprises an electromagnet or a permanent magnet.
5. The microfluidic chip analyzer according to claim 1, wherein a groove is provided on the tray, the groove matches with an outline of the microfluidic chip to form the preset position, and a projection of the magnetic member is within an information extraction chamber of the microfluidic chip.
6. The microfluidic chip analyzer according to claim 5, wherein a length D of the magnetic member in a circumferential direction of the centrifugal rotation and a length L of the information extraction chamber in the circumferential direction of the centrifugal rotation satisfy a relationship: 2L > D > 1/4L.
7. The microfluidic chip analyzer according to claim 5, wherein a length C of the magnetic member in a radial direction of centrifugal rotation and a length H of the information extraction chamber in a radial direction of centrifugal rotation satisfy a relationship: 1.2H > C > 0.8H.
8. The microfluidic chip analyzer of claim 5, wherein the outer edges of the grooves are further provided with a plurality of elastic clamping members at intervals, the elastic clamping members being used for defining the positions of the microfluidic chips.
9. The microfluidic chip analyzer of claim 4, wherein the electromagnet is disposed on the frame by a control assembly, the control assembly comprising a controller and a switch connected to the controller, the switch being connected to the electromagnet to control magnetization of the electromagnet according to a signal from the controller.
10. The microfluidic chip analyzer according to claim 5, wherein the microfluidic chip comprises a chip body, the information extraction chamber is disposed in the chip body, a temperature-controlled air chamber and an amplification chamber are further disposed in the chip body, the temperature-controlled air chamber is used for sucking liquid in the information extraction chamber, and the amplification chamber is used for amplifying nucleic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310798165.7A CN116836775A (en) | 2023-06-30 | 2023-06-30 | Microfluidic chip analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310798165.7A CN116836775A (en) | 2023-06-30 | 2023-06-30 | Microfluidic chip analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116836775A true CN116836775A (en) | 2023-10-03 |
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ID=88162891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310798165.7A Withdrawn CN116836775A (en) | 2023-06-30 | 2023-06-30 | Microfluidic chip analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116836775A (en) |
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2023
- 2023-06-30 CN CN202310798165.7A patent/CN116836775A/en not_active Withdrawn
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Application publication date: 20231003 |