CN116831178B - Application of feather degradation liquid in orange fresh-keeping - Google Patents
Application of feather degradation liquid in orange fresh-keeping Download PDFInfo
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- CN116831178B CN116831178B CN202310774131.4A CN202310774131A CN116831178B CN 116831178 B CN116831178 B CN 116831178B CN 202310774131 A CN202310774131 A CN 202310774131A CN 116831178 B CN116831178 B CN 116831178B
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- liquid
- feather
- bacillus subtilis
- citrus fruits
- degradation liquid
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- 210000003746 feather Anatomy 0.000 title claims abstract description 58
- 239000007788 liquid Substances 0.000 title claims abstract description 35
- 230000015556 catabolic process Effects 0.000 title claims abstract description 31
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 31
- 235000020971 citrus fruits Nutrition 0.000 claims abstract description 30
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 21
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 21
- 238000004321 preservation Methods 0.000 claims abstract description 18
- 241000287828 Gallus gallus Species 0.000 claims abstract description 17
- 241000207199 Citrus Species 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 230000000593 degrading effect Effects 0.000 claims description 11
- 239000001963 growth medium Substances 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 2
- 235000012054 meals Nutrition 0.000 claims 1
- 239000002609 medium Substances 0.000 claims 1
- 235000013399 edible fruits Nutrition 0.000 abstract description 18
- 239000006228 supernatant Substances 0.000 abstract description 8
- 239000003755 preservative agent Substances 0.000 abstract description 6
- 230000002335 preservative effect Effects 0.000 abstract description 6
- 102000015636 Oligopeptides Human genes 0.000 abstract description 5
- 108010038807 Oligopeptides Proteins 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 229930000044 secondary metabolite Natural products 0.000 abstract description 4
- 241000228143 Penicillium Species 0.000 abstract description 3
- 230000008859 change Effects 0.000 abstract description 3
- 230000000843 anti-fungal effect Effects 0.000 abstract description 2
- 230000000975 bioactive effect Effects 0.000 abstract description 2
- 230000005764 inhibitory process Effects 0.000 abstract description 2
- 230000028327 secretion Effects 0.000 abstract description 2
- 239000007921 spray Substances 0.000 abstract description 2
- 239000002932 luster Substances 0.000 abstract 1
- 239000002699 waste material Substances 0.000 description 10
- 244000005700 microbiome Species 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 230000002906 microbiologic effect Effects 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 102000011782 Keratins Human genes 0.000 description 2
- 108010076876 Keratins Proteins 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 206010019133 Hangover Diseases 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000005536 corrosion prevention Methods 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000007888 film coating Substances 0.000 description 1
- 238000009501 film coating Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000013332 literature search Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of feather degradation liquid in citrus preservation. The invention sprays feather degradation liquid on the outer surface of picked orange fruit uniformly, and observes the apparent change condition of the fruit in the natural state at 25 ℃. The orange fruit has bright appearance and luster within 25 days without obvious difference. At 45 days, the citrus fruits of the control group are obviously dehydrated, the surfaces of the citrus fruits are full of penicillium, and the citrus fruits are not edible; the orange fruit is bright in appearance, the peel is wrinkled, and only obvious physiological water loss occurs when the supernatant of the chicken feather degradation liquid is sprayed. The supernatant of the chicken feather degradation liquid has a certain inhibition effect on the mildew and the spoilage of citrus fruits. This may be due to bacillus subtilis forming bioactive oligopeptides during degradation of chicken feathers and/or secretion of secondary metabolites with antifungal activity. The feather degradation liquid preservative can obviously prolong the shelf life of the picked citrus fruits.
Description
Technical Field
The invention belongs to the technical field of fruit preservation, and particularly relates to application of feather degradation liquid in citrus preservation.
Background
Citrus is an important economic fruit in tropical subtropical zone, the annual output of the world is more than 1.5 hundred million tons, and the citrus is also a large agricultural product resource in China. The fruit is rich in nutrients such as saccharides, organic acids, vitamins, minerals, and carotenoids, and has important nutritional value, and has health promotion effects such as preventing and treating human diseases, relieving hangover, and relieving fatigue, and skin caring value. However, citrus fruits are susceptible to attack by pathogenic bacteria such as penicillium and viridae during post-harvest storage, resulting in fruit rot due to high water content, rich nutrition and low acidity, and in our country citrus losses are therefore between 10 and 30%. Meanwhile, the fruits tend to lose moisture and consume nutrients during storage, thereby reducing the flavor of the fruits. At present, various measures are adopted to preserve citrus fruits after picking, such as physical preservation (low-temperature storage, heat treatment, short-wave ultraviolet irradiation, liquid medicine soaking, ozone treatment and the like), chemical preservation (preservative, ethylene remover, film coating preservative and the like) and biological preservation (natural extract, antagonistic microorganisms, secondary metabolites thereof and the like). At present, physical preservation and chemical preservation have the problems of high cost, complex process, or harm to food safety or environment, and biological preservation is widely paid attention to due to nature, green and safety. Therefore, a novel safe anti-corrosion fresh-keeping technology is needed after the citrus fruits are picked.
Feathers contain about 90% keratin, and because of the rich disulfide bonds and hydrogen bonds, the keratin structure folds compactly, making the feathers insoluble in water and difficult to degrade. This also results in only a few microorganisms being able to degrade feathers. Oligopeptides produced after degradation of waste feathers by microorganisms have been reported to have a certain oxidation resistance (kssetri et al, 2022). However, it is not known whether waste feather degradation solutions (including oligopeptides produced) have a preservative effect on harvested citrus fruits.
The research on both degradation and spraying preservation of waste feathers at home and abroad is reported by related literature search, but the research on applying degradation liquid of microbial degradation waste feathers to post-harvest preservation of citrus fruits is not reported yet.
Disclosure of Invention
In order to overcome the problems, the invention provides application of the feather degradation liquid in citrus preservation. The invention utilizes microorganisms to degrade waste feathers to form mixed liquor containing amino acid, oligopeptide and secondary metabolite, and the mixed liquor is centrifugally sterilized to obtain the citrus preservative; the preservative agent is sprayed on the surfaces of citrus fruits to achieve the effects of preservation and fresh-keeping.
The aim of the invention is achieved by the following technical scheme:
the invention provides application of bacillus subtilis feather degradation liquid in citrus preservation.
Preferably, the bacillus subtilis is bacillus subtilis HC2 deposited at the cantonese province microbiological bacterial deposit center (GDMCC) at 2022, 5/12, address: guangzhou city first middle road 100 # college 59 # building 5, post code: 510070 accession number GDMCC No:62466.
preferably, the feather degrading liquid is obtained by inoculating bacillus subtilis into a culture medium containing feathers for fermentation culture.
Preferably, the feathers are chicken feathers.
Preferably, the culture medium containing feathers comprises 0.5% of monopotassium phosphate, 0.3% of dipotassium phosphate, 0.005% of magnesium sulfate, 1.0% of chicken feathers, and the balance of water according to mass fraction, wherein the pH value is 9.0.
Preferably, the feather degrading liquid is prepared by taking bacillus subtilis HC2 into a liquid LB culture medium, culturing for 16 hours at 37 ℃ and 180rpm as seed liquid, transferring the seed liquid into a chicken feather degrading culture medium in a volume ratio of 5%, and culturing for 1 day at 37 ℃ and 180 rpm.
Preferably, the application is to spray the feather degrading liquid on the surface of citrus fruits.
Compared with the prior art, the invention has the following advantages:
1. compared with most of microbial degradation feathers (more than 2 days), the microbial degradation method provided by the invention can realize efficient degradation of waste feathers in about 24 hours.
2. The technology for degrading the waste feathers is simple, and the supernatant of the feather degradation liquid is sprayed to citrus fruits to effectively play a role in corrosion prevention and preservation, and the preservation technology is simple.
Bacillus subtilis (Bacillus subtilis) HC2 deposited at the cantonese province microbiological bacterial deposit center (GDMCC) at 5/12 of 2022, address: guangzhou city first middle road 100 # college 59 # building 5, post code: 510070 accession number GDMCC No:62466.
drawings
FIG. 1 shows the screening of feather degrading bacteria to degrade feathers.
FIG. 2 shows the apparent change of the harvested fruit after spraying the supernatant of the feather degrading liquid.
Detailed Description
The invention is further explained below in connection with the drawings and the specific embodiments of the specification without any limitation thereof.
Bacillus subtilis (Bacillus subtilis) HC2 deposited at the cantonese province microbiological bacterial deposit center (GDMCC) at 5/12 of 2022, address: guangzhou city first middle road 100 # college 59 # building 5, post code: 510070 accession number GDMCC No:62466.
example 1 Bacillus subtilis degradation of waste feathers
Bacillus subtilis HC2 is picked into a liquid LB culture medium to be cultured for 16 hours at 37 ℃ and 180rpm to serve as seed liquid, and is transferred into a chicken feather degradation culture medium in an inoculum size of 5% by volume, and most of chicken feathers are degraded when being cultured for 1 day at 37 ℃ and 180rpm to obtain feather degradation liquid. As shown in FIG. 1, the degradation rate of the bacillus subtilis HC2 bacteria on the waste chicken feathers reaches 60% in 1 day; meanwhile, the bacillus subtilis HC2 bacteria grow well, and the biomass (OD 600 ) Up to 5.07, the soluble protein up to 1.96g/L, 3.45 times higher than the control group added with the same volume of sterile water. Therefore, the bacillus subtilis HC2 can efficiently degrade the waste chicken feathers.
The chicken feather degrading culture medium consists of potassium dihydrogen phosphate 0.5 wt%, dipotassium hydrogen phosphate 0.3 wt%, magnesium sulfate 0.005 wt%, chicken feather 1.0 wt% and water for the rest with pH of 9.0, and is prepared through mixing the components homogeneously, regulating pH value and sterilizing at 115 deg.c for 20 min.
Example 2 preservation of citrus fruit by feather degradation liquid
The feather degradation solution of example 1 was centrifuged at 8000rpm for 10 minutes to obtain a supernatant. Uniformly spraying the supernatant on the outer surface of the picked orange fruits, and uniformly spraying sterile water on the outer surface of the orange fruits of the control group until the outer surface of the orange fruits are subjected to fruit peel dripping. The orange fruits of the experimental group and the control group were placed in a natural state at 25 ℃ to observe the apparent change condition of the fruits. As shown in FIG. 2, the citrus fruit had a bright appearance and color over a period of 25 days without significant differences. At 45 days, the citrus fruits of the control group are obviously dehydrated, the surfaces of the citrus fruits are full of penicillium, and the citrus fruits are not edible; the orange fruit is bright in appearance, the peel is wrinkled, and only obvious physiological water loss occurs when the supernatant of the chicken feather degradation liquid is sprayed. The supernatant of the chicken feather degradation liquid has a certain inhibition effect on the mildew and the spoilage of citrus fruits. This may be due to bacillus subtilis forming bioactive oligopeptides during degradation of chicken feathers and/or secretion of secondary metabolites with antifungal activity. The feather degradation liquid preservative can obviously prolong the shelf life of the picked citrus fruits.
The above examples are merely specific embodiments of the present invention, but the inventive concept is not limited thereto. Insubstantial modifications from this concept should fall within the scope of the invention.
Claims (6)
1. Application of feather degradation liquid of bacillus subtilis in citrus preservation, wherein the bacillus subtilis is bacillus subtilis @Bacillus subtilis) HC2, accession number GDMCC No:62466.
2. the use according to claim 1, wherein the feather degrading liquid is obtained by fermentation culture of bacillus subtilis in a culture medium containing feathers.
3. The use according to claim 2, wherein the feathers are chicken feathers.
4. The use according to claim 2, wherein the feathered culture medium comprises, by mass fraction, 0.5% potassium dihydrogen phosphate, 0.3% dipotassium hydrogen phosphate, 0.005% magnesium sulfate, 1.0% chicken feather meal, the balance being water, pH 9.0.
5. The method according to claim 4, wherein the feather degrading liquid is obtained by culturing bacillus subtilis HC2 in liquid LB medium at 37 ℃ and 180 r/min for 16h as seed liquid, transferring the seed liquid into a culture medium containing feathers in an inoculum size of 5% by volume, and culturing the seed liquid at 35 ℃ and 180rpm for 1 day.
6. The use according to claim 1, wherein the application is spraying of a feather degrading liquid onto the surface of citrus fruit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310774131.4A CN116831178B (en) | 2023-06-28 | 2023-06-28 | Application of feather degradation liquid in orange fresh-keeping |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310774131.4A CN116831178B (en) | 2023-06-28 | 2023-06-28 | Application of feather degradation liquid in orange fresh-keeping |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116831178A CN116831178A (en) | 2023-10-03 |
| CN116831178B true CN116831178B (en) | 2024-03-12 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310774131.4A Active CN116831178B (en) | 2023-06-28 | 2023-06-28 | Application of feather degradation liquid in orange fresh-keeping |
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| Country | Link |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114958690A (en) * | 2022-06-28 | 2022-08-30 | 广东海洋大学 | Bacillus, screening method thereof and application of bacillus in efficient feather degradation |
| CN115094002A (en) * | 2022-06-28 | 2022-09-23 | 广东海洋大学 | Marine microorganism antagonistic to pathogenic bacteria penicillium digitatum of citrus green mold as well as screening method and application of marine microorganism |
| CN115316496A (en) * | 2022-08-30 | 2022-11-11 | 广东省科学院生物与医学工程研究所 | Method for preparing high-protein probiotic animal feed by using waste feathers |
| CN115975857A (en) * | 2022-10-18 | 2023-04-18 | 广东省科学院生物与医学工程研究所 | Broad-spectrum culture medium suitable for degrading waste feathers and application thereof |
| CN115992065A (en) * | 2022-08-30 | 2023-04-21 | 广东省科学院生物与医学工程研究所 | Method for preparing animal feed by bioconversion of waste feathers |
-
2023
- 2023-06-28 CN CN202310774131.4A patent/CN116831178B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114958690A (en) * | 2022-06-28 | 2022-08-30 | 广东海洋大学 | Bacillus, screening method thereof and application of bacillus in efficient feather degradation |
| CN115094002A (en) * | 2022-06-28 | 2022-09-23 | 广东海洋大学 | Marine microorganism antagonistic to pathogenic bacteria penicillium digitatum of citrus green mold as well as screening method and application of marine microorganism |
| CN115316496A (en) * | 2022-08-30 | 2022-11-11 | 广东省科学院生物与医学工程研究所 | Method for preparing high-protein probiotic animal feed by using waste feathers |
| CN115992065A (en) * | 2022-08-30 | 2023-04-21 | 广东省科学院生物与医学工程研究所 | Method for preparing animal feed by bioconversion of waste feathers |
| CN115975857A (en) * | 2022-10-18 | 2023-04-18 | 广东省科学院生物与医学工程研究所 | Broad-spectrum culture medium suitable for degrading waste feathers and application thereof |
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| 两株芽孢杆菌降解羽毛比较及抗氧化肽分离;李雷，等;应用与环境生物学报;20180225;24(第01期);172-176 * |
| 几种重要水果采后病害拮抗细菌的筛选与初步鉴定;代鹏，等;中国南方果树;20111120;40(第06期);59-62 * |
| 枯草芽孢杆菌(Bacillus subtilis)B-912对采后柑桔果实青、绿霉病的抑制效果;范青，等;植物病理学报;20001230;30(第04期);343-348 * |
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| CN116831178A (en) | 2023-10-03 |
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