CN116814736A - Quality control method and system for liquid taking of microbial sample inoculating loop - Google Patents
Quality control method and system for liquid taking of microbial sample inoculating loop Download PDFInfo
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- CN116814736A CN116814736A CN202310922845.5A CN202310922845A CN116814736A CN 116814736 A CN116814736 A CN 116814736A CN 202310922845 A CN202310922845 A CN 202310922845A CN 116814736 A CN116814736 A CN 116814736A
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- 239000007788 liquid Substances 0.000 title claims abstract description 44
- 238000003908 quality control method Methods 0.000 title claims abstract description 17
- 230000000813 microbial effect Effects 0.000 title claims description 19
- 230000007246 mechanism Effects 0.000 claims abstract description 96
- 238000011081 inoculation Methods 0.000 claims abstract description 59
- 238000001514 detection method Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 238000005259 measurement Methods 0.000 claims description 3
- 230000002906 microbiologic effect Effects 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims 4
- 244000005700 microbiome Species 0.000 abstract description 6
- 238000012545 processing Methods 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005452 bending Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
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Abstract
The invention belongs to the technical field of medical microorganism sample processing equipment, and particularly discloses a quality control method and a system for taking liquid from an inoculating loop of a microorganism sample. The inoculating needle is controlled by the inoculating mechanism to be placed in the sample cup for taking liquid, and picture information of an inoculating loop after taking liquid is collected; the inoculating needle is moved to an inoculating position by an inoculating mechanism, inoculating is completed on the surface of the culture dish, and picture information of an inoculating loop is obtained; and comparing each picture information of the inoculating loop with a preset inoculating loop state picture to obtain an inoculating loop liquid taking state, judging whether the inoculating loop is successful in liquid taking and whether a liquid film is successfully broken after inoculation, and outputting a judging result. By adopting the technical scheme, the liquid taking quality of the inoculating loop is ensured by detecting each operation state of the inoculating loop.
Description
Technical Field
The invention belongs to the technical field of medical microorganism sample processing equipment, and relates to a quality control method and a system for liquid taking of a microorganism sample inoculating loop.
Background
Currently, bacterial inoculation of solid media in the microbiological industry is also accomplished manually, mainly by hand. Most of microbial sample treatment is manually operated inoculation, the requirement on the personal operation level is high, aseptic operation is not only needed, but also ideal inoculation effect can be obtained through strict training and long-term operation practice. Secondly, the artificial inoculation method has low overall efficiency, is influenced by personal level and inoculation habit of operators, and partial operators may have situations of delay or slow action, so that bacteria dipped on an inoculation ring are exposed in the air for a long time to influence the bacterial culture effect.
In a microbial sample processing system, accurate sample removal from a sample cup by an automated means and effective inoculation onto the surface of a culture dish is the most important ring in the whole system, and the situation of failure or non-ideal inoculation is common and not easy to find, and is not monitored, controlled and complicated in structure in the prior art, and the inoculation ring is not subjected to position determination, so that sample processing is not accurate and effective. If the control is needed, a great amount of labor cost is increased, and the problems of environmental pollution and the like exist.
Disclosure of Invention
The invention aims to provide a method and a system for controlling the liquid taking quality of a microbial sample inoculating loop, which realize sampling monitoring of the inoculating loop and ensure the liquid taking quality of the inoculating loop.
In order to achieve the above purpose, the basic scheme of the invention is as follows: a quality control method for a microbial sample inoculating loop liquid taking method comprises the following steps:
the inoculating mechanism picks up the inoculating needle, judges whether the length and the shape of the inoculating loop meet the requirements, if the length and the shape of the inoculating loop meet the requirements, judges the liquid taking quality of the inoculating loop, and specifically judges the liquid taking quality of the inoculating loop as follows:
the inoculating needle is controlled by the inoculating mechanism to be placed in the sample cup for taking liquid, and picture information of an inoculating loop after taking liquid is collected;
the inoculating needle is moved to an inoculating position by an inoculating mechanism, inoculating is completed on the surface of the culture dish, and picture information of an inoculating loop is obtained;
and comparing each picture information of the inoculating loop with a preset inoculating loop state picture to obtain an inoculating loop liquid taking state, judging whether the inoculating loop is successful in liquid taking and whether a liquid film is successfully broken after inoculation, and outputting a judging result.
The working principle and the beneficial effects of the basic scheme are as follows: according to the technical scheme, after each time of grabbing the inoculating needle, the inoculating mechanism detects whether the inoculating loop deforms or displaces, and judges the actual position and the shape of the inoculating loop, so that manual adjustment is performed when the state of the inoculating loop is abnormal, and subsequent inoculation is not influenced. And then accurately placing the positioned inoculating loop in the sample cup, monitoring the state of the inoculating loop for extracting the sample and the subsequent inoculating state, ensuring that the sample is effectively taken out, improving the accuracy of subsequent detection and ensuring the quality of liquid taken by the inoculating loop.
Further, the specific method for judging whether the length and the shape of the inoculating loop meet the standards is as follows:
the inoculating mechanism picks up a preset standard inoculating needle and drives the inoculating needle to move above the positioning mechanism, the inoculating mechanism controls the preset standard inoculating needle to move vertically and/or transversely for a certain distance, the length and curvature information of an inoculating loop on the preset standard inoculating needle are collected, and the mechanism controls the distance information of the preset standard inoculating needle to move and is used as an initial calibration value;
the inoculation mechanism picks up the actual inoculation needle and drives the actual inoculation needle to move above the positioning mechanism, the inoculation mechanism controls the actual inoculation needle to move vertically and/or transversely for a certain distance, the length and curvature information of an inoculation ring on the actual inoculation needle are collected, and the mechanism controls the distance information of the actual inoculation needle to move as an actual measurement value;
and comparing the measured value with the initial calibration value, and judging that the form of the inoculating loop of the implementing inoculating needle does not accord with the standard if the difference value between the measured value and the initial calibration value exceeds the preset range.
Judging the form of the inoculating loop, and has simple operation and convenient use.
Further, the distance of the lateral movement is 1mm-20mm.
And a certain distance value is set to meet the detection requirement of the inoculating loop.
The invention also provides a microbial sample inoculating loop liquid taking quality control system based on the method, which comprises a rack, an inoculating mechanism and a culture dish which are arranged on the rack, and a positioning mechanism, an inoculating needle carrying the inoculating loop and a sample cup for storing samples which are arranged on the side edge of the rack;
the inoculation mechanism can move in the horizontal direction and the vertical direction, the detection port of the positioning mechanism faces the sample cup and is located a certain distance above the sample cup, and the inoculation mechanism can grasp an inoculation needle and can carry an inoculation ring to sequentially move to the sample cup and the culture dish.
According to the technical scheme, after each time the inoculating needle is grabbed by the inoculating mechanism, the inoculating loop on the inoculating needle is placed at the position of the detecting port of the positioning mechanism, whether the inoculating loop is deformed or displaced is detected, the actual position of the inoculating loop is judged, then the positioned inoculating loop is accurately placed in the sample position of the sample in the sample cup, the sample is effectively taken out, and the accuracy of subsequent detection is improved.
Further, the positioning mechanism comprises a photoelectric sensor, and the central sensing position of the photoelectric sensor is positioned on the side edge of the sample cup and is positioned above the sample cup by a certain distance.
The photoelectric sensor has simple structure and good performance, and is beneficial to use.
Further, the inoculation mechanism comprises a clamping piece, an x-axis moving mechanism, a y-axis moving mechanism and a z-axis moving mechanism;
the clamping piece is arranged on a moving piece of the z-axis moving mechanism, the z-axis moving mechanism is arranged on a moving piece of the x-axis moving mechanism, and the x-axis moving mechanism is arranged on a moving piece of the y-axis moving mechanism;
the culture dish, the positioning mechanism, the inoculating needle, the inoculating loop and the sample cup are all located in the moving range of the clamping piece.
The plurality of moving mechanisms are arranged to meet the moving requirement of the clamping piece, so that inoculation detection operation is smoothly carried out.
Further, the camera also comprises at least one camera, and the camera is installed on the rack.
The inoculation result is obtained by photographing through the camera, the situation that the culture dish needs to be opened to observe the inoculation result is avoided, and the risk of polluting the environment by bacteria is avoided. Meanwhile, the condition of failure or non-ideal inoculation is monitored by the camera, so that timely discovery and management are facilitated, and the success rate of inoculation of the microorganism sample is accurately judged.
Further, the camera also comprises a display, wherein the display is arranged on the frame, and the input end of the display is connected with the output end of the camera.
The display displays the shot culture dish image, so that the display is convenient to view.
Drawings
FIG. 1 is a schematic diagram of the structure of the liquid-taking quality control system of the microbial sample inoculating loop of the present invention.
Reference numerals in the drawings of the specification include: a photoelectric sensor 1, an inoculating needle 2, an inoculating loop 3, a culture dish 4, a camera 5 and a sample cup 6.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the invention.
In the description of the present invention, it should be understood that the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present invention and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.
In the description of the present invention, unless otherwise specified and defined, it should be noted that the terms "mounted," "connected," and "coupled" are to be construed broadly, and may be, for example, mechanical or electrical, or may be in communication with each other between two elements, directly or indirectly through intermediaries, as would be understood by those skilled in the art, in view of the specific meaning of the terms described above.
The invention discloses a quality control method for liquid taking of a microbial sample inoculating loop, which comprises the following steps:
the inoculating mechanism picks up the inoculating needle, judges whether the length and the shape of the inoculating loop meet the requirements, if the length and the shape of the inoculating loop meet the requirements, judges the liquid taking quality of the inoculating loop, and specifically judges the liquid taking quality of the inoculating loop as follows:
the inoculating needle is controlled by the inoculating mechanism to be placed in the sample cup for taking liquid, and picture information of an inoculating loop after taking liquid is collected;
the inoculating needle is moved to an inoculating position at a constant speed by an inoculating mechanism, inoculating is completed on the surface of the culture dish, and picture information of an inoculating loop is obtained;
and comparing each picture information of the inoculating loop with a preset inoculating loop state picture to obtain an inoculating loop liquid taking state, judging whether the inoculating loop is successful in liquid taking and whether a liquid film is successfully broken after inoculation, and outputting a judging result.
After each time of grabbing the inoculating needle, the inoculating mechanism detects whether the inoculating loop deforms or displaces, and judges the actual position and shape of the inoculating loop, so that manual adjustment is performed when the state of the inoculating loop is abnormal, and subsequent inoculation is not influenced. And then accurately placing the positioned inoculating loop in the sample cup, monitoring the state of the inoculating loop for extracting the sample and the subsequent inoculating state, ensuring that the sample is effectively taken out, improving the accuracy of subsequent detection, ensuring the liquid taking quality of the inoculating loop, and reducing the labor consumption.
In a preferred scheme of the invention, the specific method for judging whether the length and the shape of the inoculating loop meet the standards is as follows:
the inoculating mechanism picks up a preset standard inoculating needle and drives the inoculating needle to move above the positioning mechanism, the inoculating mechanism controls the preset standard inoculating needle to move vertically and/or transversely for a certain distance (preferably, the distance length of the transverse movement is 1mm-20mm, a certain distance value is set to meet the detecting requirement of an inoculating loop), the length and the curvature information of the inoculating loop on the preset standard inoculating needle are collected, and the mechanism controls the distance information of the movement of the preset standard inoculating needle to serve as an initial calibration value;
the inoculation mechanism picks up the inoculation needle for implementation and drives the inoculation needle to move above the positioning mechanism (namely the central sensing position of the photoelectric sensor) at a constant speed, the inoculation mechanism controls the inoculation needle for implementation to move vertically and/or transversely for a certain distance, the length and curvature information of an inoculation ring on the inoculation needle for implementation are collected, and the mechanism controls the distance information of the movement of the inoculation needle for implementation to serve as actual measurement values;
and comparing the measured value with the initial calibration value, if the difference value between the measured value and the initial calibration value exceeds the preset range, indicating that the loop has bending or the length cannot meet the requirement, judging that the form of the loop for implementing the inoculating needle does not meet the standard, and manually treating or replacing the loop.
The invention also provides a microbial sample inoculating loop liquid taking quality control system based on the method, which is shown in figure 1 and comprises a rack, an inoculating mechanism and a culture dish 4 which are arranged on the rack, and a positioning mechanism, an inoculating needle 2 carrying the inoculating loop 3 and a sample cup 6 for storing samples which are arranged on the side edge of the rack, wherein the positioning mechanism and the sample cup 6 are not mutually interfered.
The inoculating mechanism can move in the horizontal direction and the vertical direction, the inoculating mechanism can grasp the inoculating needle 2 and can carry the inoculating loop 3 to sequentially move to the sample cup 6 and the culture dish 4, and the inoculating mechanism comprises a clamping piece, an x-axis moving mechanism, a y-axis moving mechanism and a z-axis moving mechanism. The clamping member is arranged on a moving member of the z-axis moving mechanism, the z-axis moving mechanism is arranged on a moving member of the x-axis moving mechanism, and the x-axis moving mechanism is arranged on a moving member of the y-axis moving mechanism. The culture dish 4, the positioning mechanism, the inoculating needle 2, the inoculating loop 3 and the sample cup 6 are all located within the moving range of the gripping member.
The clamping piece can adopt an electric clamping device, and the x-axis moving mechanism, the y-axis moving mechanism and the z-axis moving mechanism can adopt the existing electric screw rod structure to carry out linear reciprocating movement. The moving mechanism is arranged to meet the moving requirement of the clamping piece, so that inoculation detection operation is smoothly carried out. The plurality of moving mechanisms are arranged to meet the moving requirement of the clamping piece, so that inoculation detection operation is smoothly carried out.
The detection port of the positioning mechanism faces the sample cup 6 and is located at a certain distance above the sample cup 6, the positioning mechanism comprises a photoelectric sensor 1 (such as FT series, BEN500-DDT and the like), and the central sensing position of the photoelectric sensor 1 is located at the side edge of the sample cup 6 and is located at a certain distance above the sample cup 6. The photoelectric sensor 1 has simple structure and good performance and is beneficial to use
After grabbing the inoculating needle 2 each time through the inoculating mechanism, placing the inoculating loop 3 on the inoculating needle 2 at the position of the detecting port of the positioning mechanism, detecting whether the inoculating loop 3 is deformed or displaced, judging the actual position of the inoculating loop 3, accurately placing the positioned inoculating loop 3 into the sample position of the sample cup 6, effectively taking out the sample, and improving the accuracy of subsequent detection.
In a preferred scheme of the invention, the liquid taking quality control system of the microbial sample inoculating loop 3 further comprises at least one camera 5, and the camera 5 is fixedly mounted (e.g. riveted, bonded and the like) on the frame. The inoculation result is obtained by photographing through the camera 5, the situation that the culture dish 4 needs to be opened to observe the inoculation result is avoided, and the risk of polluting the environment by bacteria is avoided. Meanwhile, the camera 5 is used for monitoring the condition of failure or non-ideal inoculation, so that timely discovery and management are facilitated, and the success rate of inoculation of the microorganism sample is accurately judged. Through the cooperation of photoelectric sensor 1 and camera 5, accurate judgement inoculating loop 3 and the effectual contact of culture dish 4 surface to effectively inoculate the culture dish 4 surface with the bacterium that inoculating loop 3 was taken.
Preferably, the liquid-taking quality control system of the microbial sample inoculating loop 3 further comprises a display, wherein the display is fixedly arranged (such as welding, bonding, riveting, screw connection and the like) on the frame, and the input end of the display is electrically connected with the output end of the camera 5. The display displays the shot image of the culture dish 4, so that the image is convenient to view.
The invention is safe and environment-friendly, eliminates unsafe factors in the prior art, and avoids the risk of bacteria polluting the environment by opening the culture dish 4 for observation. The operation is simple, the operation is automatically performed by the existing structure, and the operator only needs to check the final result.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that: many changes, modifications, substitutions and variations may be made to the embodiments without departing from the spirit and principles of the invention, the scope of which is defined by the claims and their equivalents.
Claims (8)
1. The quality control method for the liquid taking of the microbial sample inoculating loop is characterized by comprising the following steps of:
the inoculating mechanism picks up the inoculating needle, judges whether the length and the shape of the inoculating loop meet the requirements, if the length and the shape of the inoculating loop meet the requirements, judges the liquid taking quality of the inoculating loop, and specifically judges the liquid taking quality of the inoculating loop as follows:
the inoculating needle is controlled by the inoculating mechanism to be placed in the sample cup for taking liquid, and picture information of an inoculating loop after taking liquid is collected;
the inoculating needle is moved to an inoculating position by an inoculating mechanism, inoculating is completed on the surface of the culture dish, and picture information of an inoculating loop is obtained;
and comparing each picture information of the inoculating loop with a preset inoculating loop state picture to obtain an inoculating loop liquid taking state, judging whether the inoculating loop is successful in liquid taking and whether a liquid film is successfully broken after inoculation, and outputting a judging result.
2. The method for controlling the quality of a liquid taken from an inoculating loop for a microbial sample according to claim 1, wherein the specific method for judging whether the length and the shape of the inoculating loop meet the standards is as follows:
the inoculating mechanism picks up a preset standard inoculating needle and drives the inoculating needle to move above the positioning mechanism, the inoculating mechanism controls the preset standard inoculating needle to move vertically and/or transversely for a certain distance, the length and curvature information of an inoculating loop on the preset standard inoculating needle are collected, and the mechanism controls the distance information of the preset standard inoculating needle to move and is used as an initial calibration value;
the inoculation mechanism picks up the actual inoculation needle and drives the actual inoculation needle to move above the positioning mechanism, the inoculation mechanism controls the actual inoculation needle to move vertically and/or transversely for a certain distance, the length and curvature information of an inoculation ring on the actual inoculation needle are collected, and the mechanism controls the distance information of the actual inoculation needle to move as an actual measurement value;
and comparing the measured value with the initial calibration value, and judging that the form of the inoculating loop of the implementing inoculating needle does not accord with the standard if the difference value between the measured value and the initial calibration value exceeds the preset range.
3. The method for controlling the quality of a liquid taken from a microbial sample inoculating loop according to claim 2, wherein the length of the distance of the lateral movement is 1mm-20mm.
4. A microbiological sample inoculating loop liquid taking quality control system based on the method of one of claims 1-3, characterized by comprising a frame, an inoculating mechanism and a culture dish which are arranged on the frame, and a positioning mechanism, an inoculating needle carrying the inoculating loop and a sample cup for storing samples which are arranged on the side of the frame;
the inoculation mechanism can move in the horizontal direction and the vertical direction, the detection port of the positioning mechanism faces the sample cup and is located a certain distance above the sample cup, and the inoculation mechanism can grasp an inoculation needle and can carry an inoculation ring to sequentially move to the sample cup and the culture dish.
5. The microbial sample loop extraction quality control system of claim 4, wherein the positioning mechanism comprises a photoelectric sensor, and wherein a center sensing position of the photoelectric sensor is located at a distance above and beside the sample cup.
6. The microbial sample loop extraction quality control system of claim 4, wherein the inoculating mechanism comprises a gripper, an x-axis movement mechanism, a y-axis movement mechanism, and a z-axis movement mechanism;
the clamping piece is arranged on a moving piece of the z-axis moving mechanism, the z-axis moving mechanism is arranged on a moving piece of the x-axis moving mechanism, and the x-axis moving mechanism is arranged on a moving piece of the y-axis moving mechanism;
the culture dish, the positioning mechanism, the inoculating needle, the inoculating loop and the sample cup are all located in the moving range of the clamping piece.
7. The microbial sample loop extraction quality control system of claim 4, further comprising at least one camera mounted to the housing.
8. The microbial sample loop extraction quality control system of claim 7, further comprising a display mounted on the housing, an input of the display being connected to an output of the camera.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310922845.5A CN116814736A (en) | 2023-07-26 | 2023-07-26 | Quality control method and system for liquid taking of microbial sample inoculating loop |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310922845.5A CN116814736A (en) | 2023-07-26 | 2023-07-26 | Quality control method and system for liquid taking of microbial sample inoculating loop |
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| Publication Number | Publication Date |
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| CN116814736A true CN116814736A (en) | 2023-09-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202310922845.5A Pending CN116814736A (en) | 2023-07-26 | 2023-07-26 | Quality control method and system for liquid taking of microbial sample inoculating loop |
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| CN (1) | CN116814736A (en) |
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- 2023-07-26 CN CN202310922845.5A patent/CN116814736A/en active Pending
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